Longline fishing is the most common elasmobranch capture method in the world, both for commercial fishing, and to a lesser extent for scientific surveys. The capture of an animal on a longline initiates a series of physiological responses designed to promote survivorship in the short term, but if unchecked, can cause reduced individual fitness and/or mortality in the long term. Given widespread declines in shark populations, an improved understanding of the physiological costs of longline capture is needed. The aim of this thesis was to investigate the physiological response of sharks to capture and restraint, to assess novel, non-invasive alternatives to scientific longline surveys, and to generate scientific insight into poorly understood elasmobranch populations in The Bahamas. The results presented herein suggest that some species of shark are able to recover from the physiological stress of capture despite the presence of persistent negative stimuli. Tonic immobility was assessed as a means of generating baseline blood chemistry data, but was found to be inappropriate given that it increases the magnitude of physiological perturbation in the short term. To avoid the stress of capture altogether, Baited Remote Underwater Video Surveys (BRUVS) were considered as a non-invasive alternative to capture based surveys, however, it was concluded that they lack the resolution necessary to answer fine scale demographic questions. For the Caribbean reef shark, longline surveys yielded high resolution data allowing the identification of fine scale spatiotemporal shifts in demographic population structure with minimal cost (mortality). Nevertheless, the ethics of using capture based surveys on sensitive species are questionable when alternative techniques are available. Deep water sharks caught on longline surveys exhibited high mortality rates, however, for these very poorly understood species moribund specimens have great scientific value which in some cases can offset the high ecological costs of the surveys. The results presented in this thesis highlight the on-going need for improved biological and ecological research into the majority of elasmobranch populations, particularly with regards to anthropogenic interactions such as capture. Given the tenuous conservation status of many species, the acquisition of applied, management focused data should remain the priority of elasmobranch scientists.

Lake trout (Salvelinus namaycush) were extirpated from Lake Champlain around 1900 and from the lower four Great Lakes by 1960. Their ecological, commercial and recreational importance has prompted extensive restoration efforts. Despite widespread evidence of natural reproduction by stocked lake trout, there is minimal evidence of survival of wild progeny beyond age-0. Various abiotic and biotic impediments may be preventing self-sustaining lake trout populations from becoming established. Unsuccessful restoration in shallow areas has recently prompted a shift to restoration efforts to offshore, deep reefs in the Great Lakes. The first objective of this study was to develop, test, and implement methods for evaluating lake trout reproduction in deep water, where previously established techniques were ineffective. The second objective addressed the recruitment bottleneck between the emergent fry and juvenile life stages in Lake Champlain, by assessing the severity of predation on lake trout fry by epi-benthic fish. In order to quantify egg density on deep-water habitats (>18 m), we paired a deep-water egg trap with egg bags to establish a relationship between the two types of gear in Lake Champlain. There was no significant difference between densities in the egg bags and deep-water traps, but there was a positive correlation of their ranks (correlation coefficient = 0.514, p<0.0001). The deep-water traps were then used in Lake Michigan to successfully acquire the first egg density data from two sites on the deep Mid Lake Reef Complex. A drop electroshocker was developed to detect fry presence and tested in Lake Champlain in conjunction with emergent fry traps. Both types of gear exhibited similar patterns of fry relative abundance. To assess fry predation in Lake Champlain, two-hour gillnet sets during the period of fry emergence to identify fry predators and to describe how predation patterns changed diurnally and temporally. Seven species of epi-benthic fry predators were identified, including five species that had not been previously identified as fry predators. Yellow perch and rock bass dominated the predator community at two study sites (83% of total catch, N=1179, 77% of all fry predators, N=57). Predator presence and fry consumption was almost entirely nocturnal. There was a linear aggregational response in predator CPUE (fish/hr) to increasing fry relative abundance (p<0.033) but confirmed predators did not exhibit a functional response. There was evidence of a threshold of fry relative abundance at 1 fry/trap/day for the onset and conclusion of fry predation. Temperature was a driving factor in the timing of fry emergence and predator abundance, allowing us to predict the relative impact of predators based on temperature scenarios. Only 5% of the potential predators consumed fry. We used empirical probabilities of consumption to model loss of fry due to predation. This consumption model revealed that predator abundances would have to be extremely high for predation to significantly reduce the population of fry. However, given the relatively high species richness of predators observed at the shallow water study sites, lake trout fry survival is likely to be higher at deep, offshore reefs. These results support the recent shift in restoration efforts to focus on deep reefs.

O revestimento de níquel químico é largamente utilizado na proteção contra a corrosão de equipamentos e estruturas utilizados na exploração de petróleo em águas profundas. Apesar de ser considerado um dos revestimentos mais adequados para esta finalidade, são muitos os casos em que se verificam falhas prematuras do revestimento em serviço. Isto tem sido atribuído às diferenças nas características do revestimento decorrentes das diferenças do processo de aplicação e da natureza e preparação da superfície dos substratos a serem protegidos. Estas diferenças decorrem do fato de não existirem especificações abrangentes que definam as características necessárias do revestimento para uma garantia de qualidade. Diante disto, o presente trabalho teve como objetivo estudar a influência dos principais fatores sobre o desempenho dos revestimentos de níquel químico utilizando ferramentas estatísticas como: planejamento fatorial, seleção dos melhores subconjuntos de regressão, regressão múltipla e curvas de nível. Os fatores considerados neste estudo foram: tipo e estado superficial do substrato, idade do banho, teor de fósforo no revestimento, natureza e teor de contaminantes no revestimento, espessura do revestimento e tipo de tratamento térmico. A caracterização dos revestimentos de níquel químico foi realizada conforme sugerida na norma ISO 4527 e o desempenho dos revestimentos foi verificado por meio dos ensaios de exposição à névoa salina (1 536 h), exposição ao dióxido de enxofre (10 ciclos) e imersão em água do mar sintética (120 dias). Ensaios eletroquímicos (polarização linear e espectroscopia de impedância eletroquímica) foram realizados somente com o intuito de compará-los ao ensaio de exposição à névoa salina e,assim, ter um ensaio capaz de verificar revestimentos de bom desempenho em um período de tempo menor. Dentro dos intervalos estudados, verificou-se que o desempenho do revestimento de níquel químico é influenciado pelo tipo de substrato, pela espessura da camada depositada, pelo teor de fósforo, pela idade do banho, pelo tratamento térmico e pelos teores dos seguintes contaminantes: enxofre, chumbo, magnésio, cobre, ferro e manganês. A metodologia estatística adotada permitiu verificar a interação entre os fatores influenciadores, fato não considerado na literatura consultada. Além disto, foi possível constatar que os ensaios adotados nas normas internacionais atuais são adequados para verificar a qualidade dos revestimentos. Verificou-se também que os ensaios eletroquímicos, apesar de adequados para o estudo da resistência intrínseca à corrosão do revestimento de níquel químico, não se mostraram adequados para detectar a presença de porosidade que é o principal indicativo da qualidade de proteção por barreira de revestimentos catódicos como o níquel químico. Com os resultados obtidos, será possível a elaboração de uma especificação para se obter revestimentos de níquel químico com bom desempenho. / The autocatalytic nickel-phosphorus (NiP) or electroless nickel (EN) coatings are widely used to protect equipment and structures against corrosion and erosion, used in the exploitation of petroleum in deep waters. Even though NiP is considered as the most appropriate coating for this purpose, many premature failures are verified in service. This has been attributed to the differences in the characteristics of the coating due to the differences in the application processes and in the nature and preparation of the substrate surfaces. The lack of broad specifications that define the required characteristics of the good quality coating is responsible for those differences. The present work had as its objective to identify the main factors related to the NiP performance using statistical tools as: factorial design, best subset regression, multiple regression and contour diagram. The considered factors were: substrate type, substrate surface finishing, bath age, phosphorus content, thickness, heat treatment and impurities. The NiP coatings were characterized in agreement with the ISO 4527 and their performance was verified through the salt spray test (1 536 h), the Kesternish test (10 cycles) and the synthetic sea water immersion test (120 days). Electrochemical tests (linear polarization and electrochemical impedance spectroscopy) were conducted in order to compare them to the salt spray test and thus reducing the testing time. In the studied range, it was verified that the NiP performance is influenced by the following factors: substrate type, thickness coating, phosphorus content, bath age (MTO), heat treatment and the impurities: sulfur, lead, magnesium, copper, iron and manganese. It was possible to verify that the types of accelerated corrosion tests adopted in the current international standards are appropriate to verify the quality of the coatings. It was also verified that the electrochemical tests, despite being suitable to indicate the NiP intrinsic corrosion resistance, are not appropriate to verify the NiP porosity (which is the main indication of a cathodic coating quality). With the obtained results, it will be possible to establish a better specification in order to obtain NiP with good quality.

Populations that utilize deep water running (DWR) are described in Chapter I. A review of the literature concerning studies comparing peak physiological variables of water exercises (swimming, DWR, & land based running) to land based exercises (cycle ergometer, walking, & running) are presented in Chapter II. The protocols utilized for obtaining peak values on land and in the water along with subject characteristics, statistical methods and description of the training regimen are discussed in Chapter HI. The results, presented in Chapter IV, indicate no interaction between any of the variables measured but a main effect for treadmill V02 peak for the pre- and post testing. Chapter V discusses factors which may limit physiological changes within each training group. Chapter VI contains suggestions for further research.

Hydraulic connectivity for the Tiber field and 17 other Wilcox penetrations in Keathley Canyon (KC) and 5 fields in Walker Ridge (WR) protraction areas was assessed. All five chronostratigraphic Wilcox units are not in vertical communication across both protraction areas. Four of these units are in lateral communication across Tiber field except where faults isolate portions of the structure. Five “areas of connectivity,” where two or more fields are in communication, were found in KC. The fields in WR show no evidence of connectivity despite a relatively simpler structural environment than KC. I propose that the wells in WR are isolated due to a combination of diagenetic cementation and increased vertical effective stress acting to decrease permeability between structures. I also attempted to assess the possibility of hydrodynamic flow in the primary basin encompassing Tiber by geophysically identifying the field’s oil water contact and determining its orientation. This was unsuccessful.

The use of deep-water immersion during labour and birth is commonplace in many countries including Australia, yet there has been little contemporary Australian data from which to form policies regarding its use during childbirth, or which have included women’s experiences using water immersion. The literature reviewed for this study was positive with regard to the effect of water immersion during childbirth and was associated with decreased rates of perineal trauma, low episiotomy rates, low rates of analgesic use, lower operative deliveries coupled with increased maternal satisfaction of the experience of childbirth when compared with births where water immersion was not involved. The purpose of this research was to investigate the influence of deep-water immersion upon maternal and neonatal outcomes and women's experiences of giving birth in Australia. This study used a mixed method in an attempt to fulfil this purpose: the first phase was a Quasi-experimental design and the second phase was based upon a Hermeneutic Phenomenological approach. Data were collected via a Random Chart Audit, from a random sample of fifty nulliparous women who used deepwater immersion during labour and childbirth and six women were selected to participate in a semi-structured interview. Data from each phase of this study revealed positive birth outcomes and these findings were supported by the literature. The women's stories were positive and comprised elements of four lifeworld themes. • Water’s Embrace • Warped Time • Naked but Clothed • The Shape of Water. Each of these themes encapsulated different aspects of the women's experiences, which when considered together, increased the understanding of the phenomenon of deep-water immersion upon the experience of giving birth.

Although levee deposits make up a significant part of modern and ancient deep-marine slope systems, details of their internal lithological composition and stratal architecture remain poorly documented. At the Castle Creek study area, strata of the Neoproterozoic Isaac Formation (Windermere Supergroup) crop out superbly in a kilometre-scale section through a sinuous deep-water channel-levee system (ICC3). Levee deposits near the outer bend of the channel consist of sandstone-rich (sandstone-to-mudstone ratio of 68:42), medium- to thick-bedded turbidites interstratified with thinly-bedded turbidites. Structureless sandstone (Ta), planar laminated sandstone (Tb), non-climbing ripple cross-stratified sandstone (Tc) and massive and laminated siltstone (Td) are common. Thick beds generally thicken and then thin and fine laterally over about 300 m. Thin-bedded strata, in contrast, thin and fine negligibly over similar distances. In the distal part of the outer-bend levee (up to 700 m laterally away from the channel) strata consist predominantly of thin-bedded Tcd turbidites with a much lower sandstone-to-mudstone ratio (35:65). On the opposite side of the channel, inner-bend levee deposits are mudstone-rich, locally as low as 15:85, and consist mostly of thin-bedded, Tcd turbidites, although thicker-bedded, Ta-d turbidites are more common in the lower part of the section. Lateral thinning and fining of beds is more rapid than their outer-bend counterpart. Levee deposits of ICC3 comprise three stacked decametre-scale upward-thinning and -fining successions. Each is interpreted to record a depositional history consisting of lateral channel migration, levee deposition, channel filling, and distal levee deposition. During the early stage of increasing levee relief it is proposed that the termini of individual beds progressively backstep towards the channel margin resulting in an overall lateral thinning of the stratal profile. This interpretation notably contrasts the common assumption that levee morphology is the result of the vertical stacking of beds that dip. In addition to field studies, laboratory experiments were conducted to determine the depositional threshold of non-climbing ripple cross-stratification, which is common in levee strata of ICC3. It was determined that non-climbing ripples form when bed aggradation rates are less than 0.015 cm/sec, and most probably in flows made up of poorly sorted sediment.

Although levee deposits make up a significant part of modern and ancient deep-marine slope systems, details of their internal lithological composition and stratal architecture remain poorly documented. At the Castle Creek study area, strata of the Neoproterozoic Isaac Formation (Windermere Supergroup) crop out superbly in a kilometre-scale section through a sinuous deep-water channel-levee system (ICC3). Levee deposits near the outer bend of the channel consist of sandstone-rich (sandstone-to-mudstone ratio of 68:42), medium- to thick-bedded turbidites interstratified with thinly-bedded turbidites. Structureless sandstone (Ta), planar laminated sandstone (Tb), non-climbing ripple cross-stratified sandstone (Tc) and massive and laminated siltstone (Td) are common. Thick beds generally thicken and then thin and fine laterally over about 300 m. Thin-bedded strata, in contrast, thin and fine negligibly over similar distances. In the distal part of the outer-bend levee (up to 700 m laterally away from the channel) strata consist predominantly of thin-bedded Tcd turbidites with a much lower sandstone-to-mudstone ratio (35:65). On the opposite side of the channel, inner-bend levee deposits are mudstone-rich, locally as low as 15:85, and consist mostly of thin-bedded, Tcd turbidites, although thicker-bedded, Ta-d turbidites are more common in the lower part of the section. Lateral thinning and fining of beds is more rapid than their outer-bend counterpart. Levee deposits of ICC3 comprise three stacked decametre-scale upward-thinning and -fining successions. Each is interpreted to record a depositional history consisting of lateral channel migration, levee deposition, channel filling, and distal levee deposition. During the early stage of increasing levee relief it is proposed that the termini of individual beds progressively backstep towards the channel margin resulting in an overall lateral thinning of the stratal profile. This interpretation notably contrasts the common assumption that levee morphology is the result of the vertical stacking of beds that dip. In addition to field studies, laboratory experiments were conducted to determine the depositional threshold of non-climbing ripple cross-stratification, which is common in levee strata of ICC3. It was determined that non-climbing ripples form when bed aggradation rates are less than 0.015 cm/sec, and most probably in flows made up of poorly sorted sediment.

The Antarctic Intermediate Water (AAIW) plays an important role on controlling globally climatic change. Numbers of researchers attempted to discuss whether AAIW across the equator or notduring glacial- interglacial transitions. In this study, we analyzed Mg/Ca and £UREE/Ca on benthic foraminiferal species, Cibicidoides wuellerstorfi, from core MD052922C, which retrieved from western equatorial Pacific region, for revealing the long-term variations of Paleohydrology in intermediate water depths of tropical Pacific. Our evaluated results reveal that the Mg/Ca ratios ranging from 1.27 to 2.04 mmol/mol, and ranging between 2.2 and 3.2¢J when calculating into paleotemoeratures.Highest Mg/Ca can be observed in MIS 6. This finding implies that the present AAIW mean temperature is colder than glacials, and the present AAIW is well-ventilated than that in glacials.Higher £UREEs/Ca values are usually well correlated with old and poor ventilated water masses. In our record, higher £UREEs/Ca values are observed at MIS 5 impling that the intermediate depth water mass of the western tropical Pacific is poor ventilated when comparing to glacials.

A transect of cores ranging from 798 m to 1585 m water depth in the South Atlantic Ocean document the relative intermediate water mass nutrient geometry and stable isotopic variability of AAIW during the Younger Dryas cooling event. The data reveal concurrent delta^13 C and delta^18 O excursions of 0.59 ppt and 0.37 ppt within the core of Antarctic Intermediate Water (AAIW) centered at 11,381 calendar years before present based on radiometric age control. A portion of the delta^1 3C variability (0.22 ppt) can be explained by a shift in thermodynamic equilibrium concurrent with a drop in temperature of 1.8°C at the locus of AAIW formation. The remaining 0.37 ppt increase in delta^13 C most likely resulted from increased wind velocities, and a greater coupling between the ocean and the atmosphere at the locus of AAIW formation (increased efficiency of the thermodynamic process). Deepwater coral mounds are aggregates of corals, other organisms, their skeletal remains, and sediments that occur on the seafloor of the world’s oceans. In the Straits of Florida, these features have been referred to as lithoherms. We use digital, side-scan sonar data collected from the submarine NR-1 from an 10.9 km^2 area at ~650 m water depth to characterize quantitatively aspects of the morphology of 216. Their lengths, widths, heights, areas, orientations and concentration on the seafloor have been determined. Analysis indicates that the outlines of relatively small to medium sized lithoherms can be effectively described with a piriform function. This shape is less applicable to the largest lithoherms because they are aggregates of smaller lithoherms. Nearly all of the lithoherms studied have axes parallel to the northward flowing Florida Current, and the heads of 80 percent of these features face into the current. The shape and orientation of the lithoherms, and evidence of megaripples and scouring in the sonar data suggest that these features are formed by a unidirectional current. Following an extensive investigation of over 200 lithoherms via side-scan sonar imagery and direct observation, we have developed a qualitative model for the formation of the lithoherm type of deep-water coral mounds in the Straits of Florida. Lithoherm formation can be characterized by four main stages of development: nucleating, juvenile, mature singular, and fused. Fused lithoherms can form via transverse and/or longitudinal accretion, however, transverse accretion at the head of the mound is likely the most efficient mechanism. A comparison of lithoherm spatial relationship to local bathymetry agrees with previous observations of deep-water coral mound formations along the levied margins of density flow scour channels.