This study contains an investigation of the conditions of plausibility for the doctrine of God within the frame of reference of the modern era. This is done by means of a hermeneutic-dogmatic analysis and evaluation of the teology of Walter Kasper. The hypothesis is that Kasper does not succeed to accomodate legitimate material-dogmatic observations in his proposed structure for a doctrine on the trinity. The modern era is characterised by the demise of classical Western metaphysics and the emergence of an ontology of relation, accentuating the historicity of reality. This became clear in the turn towards subjectivity and radical autonomy, and resulted as ultimate consequence in an atheistic way of life and mode of thought. The static nature of the classical substance-orientated ontology, influenced the conceptual modelling of classical Western theology profoundly negative. With regard to the doctrine of God, thinking in terms of salvation history and within the context of ecclesiastic practice, liturgy and doxology, receded to the background, making room for highly abstract philosophical speculation. This resulted in the separation of the immanent and economic trinity and led to two separate tracts, de Deo uno and de Deo trino. This state of affairs has the implication that two modes of thought are operative, namely a more speculative, natural-theological and a salvation historical orientated mode of thought. Kasper maintains that this two modes of thought should be upheld on the condition that the analogy, as the method of natural theology, is broadened by the perspective of the historicity and event character of reality. Analogia entis (mode of thought) must be understood as analogia libertatis (mode of being). This “additional” perspective enables the formal integration of the mentioned tracts, de Deo uno and de Deo trino. In a material sense, this means turning the back on the traditional essentialistic concept of the trinity in favour of a personal, salvation-historical approach by developing de Deo uno as doctrine concerning God the Father. Taking the fundamental anthropological structure of unthematic directedness towards absolute freedom as piont of departure, Kasper arrives at a “description” of God’s essence as Absolute Person, as Absolute Freedom to love. In accordance with his emphasis on history, Kasper propagates the equal importance of assensus- and desensus-christology. He proposes a refounding of the New Testament Son of God-christology by means of an indirect christology, that implies a trinitarian context. The implicit relationality suggested in Kasper’s description of God’s essence - Absolute Freedom to love - is made explicit in the relation of unity between Father and Son, a relation that implies the unity of Father, Son and Holy Spirit in an indirect way. According to Kasper, God the Father must be understood as source and ground of this divine unity. Kasper hereby distances himself from the traditional essentialistic concept of the trinity in favour of the Orthodox point of view, although he upholds the filioque and understands the intertrinitarian processiones in terms of the Western “theologic”. Kasper maintains that these proposal is a concrete explication of Thomas by means of the insights of Richard of St Victor, encompassing the synthesis of the essentialistic and personal trinitarian traditions on a higher niveau. This claim of Kasper must be contradicted. In the first instance, due to the fact that the trinitarian relations are merely viewed in terms of origin, his concept of trinitarian unity is one-dimensional. It is a patrocentristic orientated unity of origin. An underdeveloped pneumatology is, secondly, the direct result of the above mentioned patrocentricity. Although Kasper highlights the communio nature of trinitarian unity and the personality of the Holy Spirit, these accents are not accounted for in the structure of his proposal. Thirdly, assensus- and desensus-christology does not receive equal attention. Assensus-christology is neglected. Our proposal in this regard is a trinitarian pneumatology, explicated from the departure point of the immanent trinitarian concretion of persons and the economic trinitarian creation of personhood. In this way, the intertrinitarian unity as well as the unity of immanent and economic trinity, becomes explicable as the same unity but in different configurations. At the same time, the personality of the Holy Spirit is not only mentioned, but accounted for. / Thesis (DD(Dogmatics and Christian Ethics))--University of Pretoria, 2005. / Dogmatics and Christian Ethics / unrestricted

Submitted by Automa??o e Estat?stica (sst@bczm.ufrn.br) on 2016-03-02T23:14:28Z No. of bitstreams: 1 EdinaraTarginoDeMelo_DISSERT.pdf: 2062041 bytes, checksum: b2de2a9d0f47d751e6c33c3d69b984a7 (MD5) / Approved for entry into archive by Arlan Eloi Leite Silva (eloihistoriador@yahoo.com.br) on 2016-03-04T23:26:34Z (GMT) No. of bitstreams: 1 EdinaraTarginoDeMelo_DISSERT.pdf: 2062041 bytes, checksum: b2de2a9d0f47d751e6c33c3d69b984a7 (MD5) / Made available in DSpace on 2016-03-04T23:26:34Z (GMT). No. of bitstreams: 1 EdinaraTarginoDeMelo_DISSERT.pdf: 2062041 bytes, checksum: b2de2a9d0f47d751e6c33c3d69b984a7 (MD5) Previous issue date: 2014-03-31 / Coordena??o de Aperfei?oamento de Pessoal de N?vel Superior - CAPES / No Brasil, ? grande a incid?ncia de animais pe?onhentos, dentre os quais se destacam os escorpi?es por sua import?ncia m?dica, sendo sua pe?onha fonte de diversas subst?ncias qu?micas com atividades biol?gicas e farmacol?gicas ainda n?o compreendidas, incluindo diversos pept?deos bioativos. Pept?deos antimicrobianos (PAMs) s?o componentes do sistema imune de procariotos e eucariotos utilizados como primeira linha de defesa contra micro-organismos. No presente trabalho, foi caracterizado o primeiro PAM do escorpi?o Tityus stigmurus, nomeado Stigmurina, previamente identificado por meio do transcriptoma da gl?ndula de veneno desta esp?cie. As caracter?sticas da Stigmurina foram investigadas por modelagem computacional e constru??o de dendrograma. Al?m disso, as caracter?sticas estruturais da Stigmurina foram investigadas por dicro?smo circular em ?gua, em 2, 2, 2- trifluoethanol (TFE) e em dodecil sulfato de s?dio (SDS) e os modelos refinados por simula??es de din?mica molecular. Testes in vitro foram empregados para investigar a atividade antibacteriana, antif?ngica, hemol?tica e citot?xica do veneno bruto e da Stigmurina. Os resultados mostraram que a sequ?ncia selecionada codifica uma prote?na madura com 17 res?duos de amino?cidos e o dendrograma revela um caso de converg?ncia evolutiva. Os espectros de dicro?smo demonstraram flexibilidade conformacional, predominando estrutura estendida e ??folha, al?m de not?vel capacidade de renatura??o. A pe?onha bruta n?o apresentou atividade relevante nos testes antimicrobianos para as concentra??es testadas. No entanto, a Stigmurina mostrou um amplo espectro de atividade antibacteriana, com concentra??es inibit?rias m?nimas (CIMs) entre 31,25?g/mL a 250 ?g/mL para diferentes micro-organismos, enquanto que os resultados para a avalia??o da atividade hemol?tica nestas concentra??es foram baixas. Nos estudos de citotoxicidade, a pe?onha bruta foi incapaz de reduzir a viabilidade celular em c?lulas VERO E6, por?m sua toxicidade em c?lulas SiHa foi significativamente superior, correspondendo a uma IC50 de 3,6 ?g/mL. J? nos testes com a Stigmurina, as concentra??es capazes de reduzir a viabilidade celular das c?lulas VERO E6 e SiHa em 50% foram de 275,67 ?g/mL e 212,54 ?g/mL, respectivamente.Os resultados sugerem que a Stigmurina poder ser considerada como um potencial medicamento anti-infeccioso. / In Brazil, there is a high incidence of venomous animals. Among them, scorpions are highlighted by their medical importance, and for being their venom a source of several molecules with biological and pharmacological activity not yet fully understood, including several bioactive peptides. Antimicrobial peptides (AMPs) are components of the immune system in prokaryotes and eukaryotes, used in the first line of defense against microorganisms. In the present study, we characterized the first PAM previously identified through transcriptome of the venom gland of the scorpion Tityus stigmurus, named Stigmurin. The characteristics of Stigmurin were investigated by computational modeling and construction of dendrogram. In vitro tests investigated the antibacterial, antifungal, haemolytic and cytotoxic effects of crude venom and Stigmurin. In addition, the structural characteristics of Stigmurin were investigated by circular dochroism in water, 2, 2 , 2- trifluoethanol (TFE) and sodium dodecyl sulfate (SDS) and the models were refined by molecular dynamics simulations. The results showed that the selected sequence encodes a mature protein of 17 amino acid residues and the dendrogram reveals a case of convergent evolution. The crude venom showed no antimicrobial activity, however, Stigmurin exhibited a broad spectrum of antibacterial activity, with minimal inhibitory concentrations (MIC) ranging from 31.25 and 250 ?g/mL for different strains, while the hemolytic activity at these concentrations was low. In cytotoxicity studies, the crude venom was unable to reduce cell viability in VERO E6 cells; in contrast, its activity in SiHa cells was significantly higher, corresponding to IC50 of 3.6 ?g/mL. For Stigmurin the concentration sable to decrease cell viability of Vero E6 and SiHa cells in 50% were 275.67 ?g/mL and 212.54 ?g/mL, respectively. The dichroism spectra revealed the conformational flexibility, with predominating extended and ??sheet structures, as well as a remark able renaturation ability. The results suggest that Stigmurin could be considered as a potential antiinfective drug

Submitted by Luiz Henrique (luheeslo@gmail.com) on 2015-11-27T13:27:57Z No. of bitstreams: 1 Danilo_Regis.pdf: 4810459 bytes, checksum: 9315b2ea1003e11ac10bc6c930953e9a (MD5) / Approved for entry into archive by Tha?s Teles (thaistfirmino@gmail.com) on 2015-11-27T13:34:32Z (GMT) No. of bitstreams: 1 Danilo_Regis.pdf: 4810459 bytes, checksum: 9315b2ea1003e11ac10bc6c930953e9a (MD5) / Made available in DSpace on 2015-11-27T13:34:32Z (GMT). No. of bitstreams: 1 Danilo_Regis.pdf: 4810459 bytes, checksum: 9315b2ea1003e11ac10bc6c930953e9a (MD5) Previous issue date: 2009-03 / Esta disserta??o aborda a avalia??o da qualidade de sinais de v?deo, baseada na avali- a??o objetiva e subjetiva, para v?deos utilizados em televis?o digital para dispositivos m?veis. Essa necessidade aparece com o surgimento da televis?o digital, que permite a manipula??o do v?deo para diferentes dispositivos, incluindo dispositivos m?veis. No Brasil o padr?o adotado define uma banda do espectro para a transmiss?o da televis?o para esses dispositivos, limitada em 433 kHz. Em fun??o desse limite faz-se necess?rio o ajuste de alguns par?metros, como taxa de bit e a resolu??o espacial e temporal. Para o ajuste desses par?metros usam-se transco- dificadores de v?deos, que s?o sistemas capazes de converter uma sequ?ncia de v?deo em uma outra com diferentes par?metros. Neste trabalho ? feita a redu??o espacial dos v?deos de CIF (352 ? 288) para QCIF (176 ? 144). Para a redu??o espacial de v?deo o transcodificador pode ser implementado usando vetores de movimentos, DCT ou interpolador fracional. O interpo- lador fracional foi escolhido por ter baixo custo computacional. Ele usa os filtros da m?dia, mediana, moda, sigma e m?dia ponderada, com janelas de 2 ? 2, 3 ? 3 e 4 ? 4. Para a avalia- ??o da qualidade dos v?deos transcodificados foram usados os m?todos objetivos PSNR e SSIM e o m?todo subjetivo PC. Para a avalia??o subjetiva foi utilizado o codificador H.264 para a redu??o da taxa de bit e da resolu??o temporal do v?deo, que foi apresentado em um aparelho celular.

Submitted by Jos? Henrique Henrique (jose.neves@ufvjm.edu.br) on 2017-09-20T20:31:49Z No. of bitstreams: 2 license_rdf: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5) gabriele_azevedo_cardoso.pdf: 4324631 bytes, checksum: 3efd70a64b9fe1de1234371d61299990 (MD5) / Approved for entry into archive by Rodrigo Martins Cruz (rodrigo.cruz@ufvjm.edu.br) on 2017-10-09T13:35:54Z (GMT) No. of bitstreams: 2 license_rdf: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5) gabriele_azevedo_cardoso.pdf: 4324631 bytes, checksum: 3efd70a64b9fe1de1234371d61299990 (MD5) / Made available in DSpace on 2017-10-09T13:35:54Z (GMT). No. of bitstreams: 2 license_rdf: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5) gabriele_azevedo_cardoso.pdf: 4324631 bytes, checksum: 3efd70a64b9fe1de1234371d61299990 (MD5) Previous issue date: 2017 / Coordena??o de Aperfei?oamento de Pessoal de N?vel Superior (CAPES) / Conselho Nacional de Desenvolvimento Cient?fico e Tecnol?gico (CNPq) / Funda??o de Amparo ? Pesquisa do Estado de Minas Gerais (FAPEMIG) / A necessidade de desenvolvimento de novos agentes antimicrobianos cresce ? medida que se torna maior a resist?ncia de microrganismos aos antibi?ticos usualmente empregados. Nesse sentido, os pept?deos antimicrobianos (PAMs) surgem como uma excelente alternativa para o desenvolvimento de novos antibi?ticos. O presente trabalho prop?s a s?ntese do pept?deo antimicrobiano LyeTx-I para estudos de mecanismo de a??o em membranas bacterianas, empregando diferentes t?cnicas biof?sicas e estruturais. O pept?deo LyeTx-I, composto por 24 res?duos de amino?cidos, foi isolado pela primeira vez do veneno de aracn?deos da esp?cie Lycosa erythrognata. Utilizando como t?cnicas principais a ITC e a RMN para obten??o de par?metros cin?ticos, termodin?micos e da intera??o pept?deo-membrana, foi poss?vel avaliar a rela??o estrutura e atividade do pept?deo LyeTx-I. Foram utilizadas ainda, t?cnicas complementares de CD, extravasamento de CF, fluoresc?ncia de Trp, DLS e, potencial zeta para obter informa??es adicionais acerca do modo de intera??o do pept?deo. Observou-se a predomin?ncia de conforma??o helicoidal do pept?deo LyeTx-I, tanto em meios biomim?ticos zwitteri?nicos, quanto em meios ani?nicos. Em meios ani?nicos, observou-se maior conte?do de ?-h?lice, bem como maior constante de intera??o, enquanto que em presen?a de ambientes zwitteri?nicas foram observadas menor helicidade e constante de intera??o. Os dados termodin?micos, obtidos para ambos os meios, mostraram que o processo de intera??o pept?deo-membrana ? dirigido principalmente pela componente entr?pica, uma vez que a componente ent?lpica ? menor. Os dados estruturais e termodin?micos foram coerentes com os demais estudos biof?sicos. Foi observada a partir da an?lise de extravasamento de CF maior capacidade de forma??o de poros no meio ani?nico. Os dados de fluoresc?ncia intr?nseca de Trp e de supress?o de fluoresc?ncia por acrilamida mostraram maior mudan?a de ambiente qu?mico para apolar, do res?duo de Trp-2, quando em presen?a de meio biomim?tico ani?nico. Dessa forma, o pept?deo apresenta maior capacidade de permeabilizar a membrana ani?nica. Al?m disso, o estudo comparativo entre os meios zwitteri?nicos e ani?nicos, permitiu verificar que, embora a intera??o eletrost?tica seja importante para a intera??o pept?deo-membrana, a permeabiliza??o do LyeTx-I na membrana fosfolip?dica ? fundamental para a lise celular. Dessa forma, este estudo mostra que o pept?deo LyeTx-I apresenta elevada prefer?ncia por intera??o com bicamadas fosfolip?dicas ani?nicas, o que faz dele um potencial agente bactericida. / Disserta??o (Mestrado) ? Programa de P?s-Gradua??o em Qu?mica, Universidade Federal dos Vales do Jequitinhonha e Mucuri, 2017. / The demand for the development of new antimicrobial agents increases in line with the resistance of microorganisms to the antibiotics usually employed. In this sense, antimicrobial peptides (AMPs) appear as an alternative to the classical antibiotics. The present work proposed the synthesis of the antimicrobial peptide LyeTx-I for studies of mechanism of action in bacterial membranes using a set of biophysical and structural techniques. LyeTx-I peptide is composed of 24 amino acid residues and was isolated for the first time from the venom of the Lycosa erythrognata arachnid species. In order to evaluate the structure-activity relationship of the LyeTx-I, we have employed ITC and NMR as main techniques to obtain the kinetic, thermodynamic and structural parameters of the peptide-membrane interaction. Complementary measurements of CD, CF extravasation, Trp fluorescence, DLS and zeta potential were also used as additional information about the mode of action of the peptide. The ?-helical conformation of the LyeTx-I peptide was observed either in presence of zwitterionic and anionic biomimetic media. Nevertheless, a higher ?-helix content and interaction constant was observed for LyeTx-I in all anionic media when compared to the zwitterionic environments. The thermodynamic data gathered in both media, showed that the peptide-membrane interaction is driven mainly by the entropic contributions, since the enthalpic component is smaller. The structural and thermodynamic data were consistent with the complementary biophysical experiments. It was observed from the CF extravasation a greater capacity of pore formation in the anionic medium. Intrinsic Trp fluorescence showed also a greater change of the residue of Trp-2 to the apolar chemical environment in the presence of anionic biomimetic medium. In this way, the peptide presents a higher capacity to permeabilize the anionic membrane. In addition, the comparative study between the zwitterionic and anionic media, reveals that, although the electrostatic interaction is important to the peptide-membrane interaction, the permeabilization of the LyeTx-I peptide in the phospholipid membrane is fundamental for the cellular lysis. Finally, the study clearly shows the high preference of LyeTx-I for interacting anionic phospholipid bilayers, which makes it a potential bactericidal agent.

Made available in DSpace on 2016-04-04T18:31:20Z (GMT). No. of bitstreams: 1 Edizon Eduardo Basseto Junior.pdf: 3122282 bytes, checksum: f4c584d20c71eb7d623b23f592a8a4c2 (MD5) Previous issue date: 2008-02-08 / The goal of this work is to propose a metric to analyze the quality of videos transmitted through wireless networks. A wireless network is a degenerative means to transmit data given its huge instability, which affects transmitted videos. The present proposal adds a technical network parameter, known as jitter, to clients opinion. For these studies a video was streamed through a RF emulation testbed, which can emulate the IEEE wireless networks (WLAN) 802.11b and 802.11g standards. The experiments were done by streaming a DVD, with known characteristics, from a server to a workstation, passing through the testbed. This is capable to concentrate, in a controlled way, the wireless signal into a coaxial cable. With the possibility to control the signal level of the carrier, twelve scenarios were developed and studied: video transmissions with maximum and minimum signal levels, and also with the signal level characterized as Rayleigh distribution, were done for each of the two IEEE standards measures. Under the same signal conditions, I analyzed the video streaming with UDP traffic sent together. The results confirm the action of jitter in the degeneration of the received video, which justifies the use of jitter in the proposed metric. The results also show that the 802.11g networks work a little better than 802.11b for video streaming, but none of them showed a good performance when the UDP traffic was sent together. A questionnaire has also been developed, with the purpose of analyzing the streaming subjectively. Those two factors (jitter and subjective analysis) result in an equation that can be applied to a MOS scale making it possible to numerically define the quality of the video transmission. / O objetivo deste trabalho ? propor uma m?trica de qualidade para v?deos transmitidos em redes sem fio. As redes sem fio s?o um meio de transmiss?o muito degenerativo devido ? sua instabilidade, o que afeta um v?deo transmitido. A presente proposta soma ? opini?o dos espectadores um par?metro t?cnico da rede, conhecido por jitter, e a taxa de dados do v?deo transmitido. Para os estudos realizados, um v?deo foi transmitido atrav?s de uma bancada de emula??o de r?dio freq??ncia (RF) que simula a transmiss?o das redes sem fios (WLAN) padr?es IEEE 802.11b e 802.11g. Os experimentos foram realizados a partir do streaming de um DVD, com caracter?sticas conhecidas, de um servidor para uma esta??o, atrav?s da bancada que confina o sinal transmitido de maneira controlada em um cabo coaxial. Tendo-se essa possibilidade de controlar o n?vel do sinal da portadora, foram estudadas doze situa??es. Para cada um dos padr?es IEEE foram realizadas medidas com a transmiss?o de v?deo e um n?vel m?ximo de sinal, outra com o n?vel m?nimo e uma situa??o que tem o n?vel de sinal caracterizado por uma distribui??o de Rayleigh. Para essas mesmas condi??es de sinal foi estudada a transmiss?o do v?deo mais um tr?fego UDP concorrente. Os resultados confirmam a atua??o do jitter na degenera??o do v?deo recebido, justificando sua utiliza??o na m?trica proposta. Os resultados demonstram tamb?m que existe uma pequena vantagem das redes 802.11g na transmiss?o do streaming, por?m nenhuma das redes demonstrou um desempenho satisfat?rio na presen?a de tr?fego UDP transmitido concorrentemente. Na proposta tamb?m ? desenvolvido um question?rio com a finalidade de analisar subjetivamente o streaming, gerando uma equa??o que pode ser aplicada ? escala MOS, podendo assim definir numericamente a qualidade de v?deo transmitido.

?rea de concentra??o: Qu?mica org?nica. / Submitted by Alexandre Soares (alexandredesoares@yahoo.com.br) on 2016-08-25T12:50:30Z No. of bitstreams: 1 eduardo_ferreira_coelho_junior.pdf: 3071757 bytes, checksum: e9deb6aa475b6a67aeab117cf2df4603 (MD5) / Approved for entry into archive by Rodrigo Martins Cruz (rodrigo.cruz@ufvjm.edu.br) on 2016-09-08T17:54:32Z (GMT) No. of bitstreams: 1 eduardo_ferreira_coelho_junior.pdf: 3071757 bytes, checksum: e9deb6aa475b6a67aeab117cf2df4603 (MD5) / Made available in DSpace on 2016-09-08T17:54:32Z (GMT). No. of bitstreams: 1 eduardo_ferreira_coelho_junior.pdf: 3071757 bytes, checksum: e9deb6aa475b6a67aeab117cf2df4603 (MD5) Previous issue date: 2015 / O presente trabalho prop?e a glicosila??o do pept?deo antimicrobiano HSP-1, composto por 14 res?duos de amino?cidos e isolado originalmente da esp?cie Hyla punctata (PRATES et al., 2004) empregando-se a s?ntese de pept?deos em fase s?lida associada a rea??o de cicloadi??o catalisada por cobre (SHARPLESS et al., 2002). Para isto, foi realizada a s?ntese do pept?deo HSP-1 propargilado ([PAG1]HSP-1) atrav?s da metodologia de s?ntese de pept?deo em fase s?lida (SPFS) via estrat?gia Fmoc. Ap?s a confirma??o da obten??o do [PAG1]HSP-1 por espectrometria de massa (MALDI-ToF), foi realizada a glicosila??o com as inser??es dos derivados azido acetilado de glicose e N-acetilglicosamina na presen?a de sulfato de cobre penta hidratado (CuSO4.5H2O) e de ascorbato de s?dio como agente redutor para obten??o dos glicopept?deo-triaz?is [Glc-trz-G14]HSP-1 e [GlcNAc-trz-G14]HSP-1. Os produtos das s?nteses foram purificados por cromatografia l?quida de alta efici?ncia de fase reversa (CLAE-FR) e tamb?m caracterizados por espectrometria de massa (MALDI-ToF), confirmando a forma??o regiosseletiva dos glicopept?deo-triaz?is sem produ??o de subprodutos da glicosila??o. Os estudos biol?gicos comparativos entre o pept?deo HSP-1 e de suas formas glicosiladas revelaram que as modifica??es qu?micas n?o alteraram significativamente a efic?cia do HSP-1 contra agentes bacterianos. Entretanto, os testes antif?ngicos demonstraram melhor atividade fungicida para os glicopept?deos quando comparado ao pept?deo HSP-1. Foram ainda realizados estudos conformacionais e de intera??o entre o pept?deo e os glicopept?deos com ves?culas fosfolip?dicas de car?ter zwitteri?nico (POPC) e ani?nico (POPC/POPG). Os estudos conformacionais empregando-se a t?cnica de Dicro?smo Circular (CD) revelaram menor teor de helicidade tanto em LUV?s de POPC quanto de POPC/POPG para os glicopept?deos em rela??o a HSP-1. Os estudos de intera??o foram realizados empregando-se as t?cnicas de espalhamento de luz din?mico (DLS), potencial zeta (?) e extravasamento de carboxifluoresce?na (CF). De uma maneira geral, verifica-se que a varia??o no di?metro hidrodin?mico (?Dh) para ves?culas ii zwitteri?nicas POPC e ani?nicas POPC/POPG ? maior para os glicopept?deos [Glc-trz-G14]HSP-1e [GlcNAc-trz-G14]HSP-1 em rela??o ao HSP-1. Por outro lado, a varia??o do potencial zeta tanto em ves?culas zwitteri?nicas quanto em ves?culas predominantemente negativas causada por HSP-1 foi maior em compara??o ao efeito causado pelas formas glicosiladas. E por fim, os resultados de extravasamento de carboxifluoresce?na induzida por cada esp?cie (HSP-1, [Glc-trz-G14]HSP-1 e [GlcNAc-trz-G14]HSP-1) mostrou que a capacidade l?tica dos glicopept?deos ? ligeiramente maior em ambos os meios biomim?ticos quando comparados com o pept?deo HSP-1. Assim sendo, este trabalho mostrou que a presen?a do anel triaz?lico pode ser respons?vel pela maior atividade antif?ngica dos glicopept?deos [Glc-trz-G14]HSP-1 e [GlcNAc-trz-G14]HSP-1 em rela??o ao pept?deo HSP-1. / Disserta??o (Mestrado) ? Programa de P?s-Gradua??o em Qu?mica, Universidade Federal dos Vales do Jequitinhonha e Mucuri, 2015. / ABSTRACT This work proposes the glycosylation of the antimicrobial peptide HSP-1, containing 14 amino acid residues and originally isolated from Hyla punctata species (PRATES et al., 2004) by solid phase peptides synthesis associated with cycloaddition reaction copper catalyzed (SHARPLESS et al., 2002). The synthesis of propargylated HSP-1 ([PAG1] HSP-1) was carried out by solid phase peptide synthesis using Fmoc strategy and characterized by mass spectrometry (MALDI-ToF). In order to obtain the glycopeptide triazoles [Glc-trz-G14]HSP-1 and [GlcNAc-trz-G14]HSP-1, azide derivatives acetylated glucose and N-acetylglucosamine were used in the presence of copper sulfate pentahydrate (CuSO4. 5H2O) and sodium ascorbate as a reducing agent. The products were purified by reverse phase high performance liquid chromatography (RP-HPLC) and characterized by mass spectrometry (MALDI-ToF), confirming the regioselective reaction without glycosylation secondary products. Comparative studies among HSP-1 peptide and their glycosylated forms don?t show significant changes in antibacterial assays. However, the antifungal tests have shown a significant increase in fungicidal activity for glycopeptides when compared to HSP-1 peptide. Furthermore, it were carried out conformational and interaction studies among the peptide and glycopeptides with zwitterionic (POPC) and anionic (POPC/POPG) phospholipid vesicles. The circular dichroism (CD) spectra have revealed lower helicity to glycopeptides relative HSP-1 in both zwitterionic and anionic LUV's. Interaction studies were performed employing the dynamic light scattering (DLS), zeta potential and leakage carboxyfluorescein (CF) techniques. Summing up, the hydrodynamic diameter variation (?Dh) for zwitterionic and anionic vesicles is greater for glycopeptides [Glc-trz-G14]HSP-1 and [GlcNAc-trz-G14]HSP-1 when compared with HSP-1. On the other hand, the zeta potential variation in zwitterionic or negative vesicles caused by HSP-1 was higher compared to the effect caused by glycosylated forms. Finally, the results of carboxyfluorescein leakage induced by each species (HSP- iv 1 [Glc-trz-G14] HSP-1 and [GlcNAc-trz-G14] HSP-1) showed a higher lytic capacity of glycopeptides in both media in relation to the HSP-1 peptide. Thus, it showed that the presence of triazole rings may be responsible for the higher antifungal activity of derivatives [Glc-trz-G14] HSP-1 and [GlcNAc trz-G14] HSP-1.

Submitted by Ricardo Cedraz Duque Moliterno (ricardo.moliterno@uefs.br) on 2017-03-30T21:29:02Z No. of bitstreams: 1 DISSERTACAO THAUANE-FINAL (3).pdf: 2270065 bytes, checksum: 57e7cc71a5309821de988ba9e5addcf8 (MD5) / Made available in DSpace on 2017-03-30T21:29:02Z (GMT). No. of bitstreams: 1 DISSERTACAO THAUANE-FINAL (3).pdf: 2270065 bytes, checksum: 57e7cc71a5309821de988ba9e5addcf8 (MD5) Previous issue date: 2016-12-21 / Currently, due to the various technological options, the vast access to information and the demotivation of some students, much is discussed in the literature about the way of teaching so that it becomes diversified and attractive. The teacher / classroom model with traditional teaching has leaved to be the main medium in the teaching-learning process. Faced with this new educational paradigm, the need arises to seek strategies that increase the interest, the motivation and consequently the level of learning of the students. In this sense, we have the ludic forms of teaching, such as the application of didactic games, which are increasingly present in the school environment. From this context, a didactic game, entitled "Space Track" and an educational video called "Unraveling the Universe", was created. The theme is about Planets and Dwarf Planets, Eclipse, Seasons of the Year, General Astronomy and Constellations. In particular, these tools can be used in both formal and non-formal learning environments. In this work the product was applied in five classes of Elementary School and four of the High School of the Col?gio Estadual Ferreira Pinto. The survey of prior knowledge and learning after the implementation of activities was carried out through pre- and post-tests on the above topics. / Atualmente, em virtude das v?rias op??es tecnol?gicas, do vasto acesso ? informa??o e da desmotiva??o de alguns estudantes, muito se discute na literatura sobre a forma de ensino de modo que ela se torne diversificada e atrativa. O modelo professor/sala de aula com o ensino tradicional tem deixado de ser o principal meio no processo de ensino-aprendizagem. Diante desse novo paradigma educacional, surge a necessidade de buscar estrat?gias que aumentem o interesse, a motiva??o e consequentemente o n?vel de aprendizagem dos alunos. Neste sentido, temos as formas l?dicas de ensino, como, por exemplo, a aplica??o de jogos did?ticos, que est?o cada vez mais presentes no ambiente escolar. A partir desse contexto, foi elaborado um jogo did?tico, intitulado ?Trilha Espacial? e um v?deo educativo chamado ?Desvendando o Universo?, cuja tem?tica trata sobre Planetas e Planetas An?es, Eclipse, Esta??es do Ano, Conhecimentos Gerais de Astronomia e Constela??es. Em particular, estas ferramentas podem ser utilizadas em ambientes de ensino formal e n?o formal. Neste trabalho o produto foi aplicado em cinco turmas do Ensino Fundamental e quatro do Ensino M?dio do Col?gio Estadual Ferreira Pinto. A sondagem do conhecimento pr?vio e do aprendizado ap?s a aplica??o das atividades foi realizada por meio de pr? e p?s-testes sobre os temas citados.

Title from accompanying document. / Digitized by Kansas Correctional Industries

Made available in DSpace on 2015-04-14T14:50:56Z (GMT). No. of bitstreams: 1 411926.pdf: 537628 bytes, checksum: ad84cc7d4a6b34220ad5f86256433b5f (MD5) Previous issue date: 2009-03-31 / As caderinas desempenham um papel-chave na manuten??o das ades?es c?lula-c?lula e altera??es em sua express?o tem implica??es significativas na homeostasia tecidual, estando envolvidas na progress?o do c?ncer. O RC-3095 e um antagonista sint?tico do receptor do pept?deo liberador de gastrina capaz de restringir o crescimento de tumores pela a??o inibit?ria sobre prolifera??o, aumento da motilidade e morfog?nese de c?lulas tumorais, representando uma nova e promissora estrat?gia farmacol?gica no tratamento anti-tumoral. O objetivo deste trabalho foi analisar o efeito do tratamento com RC-3095 durante a progress?o tumoral da linhagem de adenocarcinoma colorretal HT-29 sobre a express?o de caderinas dos tipos E e N. Nossos resultados corroboram achados pr?vios que demonstraram o efeito negativo do RC-3095 sobre a viabilidade celular, refor?ando seu potencial no tratamento do c?ncer colorretal. O tratamento com RC-3095, contudo, n?o alterou o perfil de express?o de caderinas durante o per?odo de 48h estudado. Neste per?odo n?o foi detectada express?o de caderina do tipo N, enquanto a express?o de caderina E diminui progressivamente independente do tratamento com RC-3095, sugerindo que a adesividade celular n?o e alvo do antagonista do receptor do pept?deo liberador de gastrina nas condi??es estudadas.

Made available in DSpace on 2016-04-28T14:57:35Z (GMT). No. of bitstreams: 1 2009 - Rodrigo da Silva Alonso.pdf: 1310457 bytes, checksum: b5de32ca4648741d0f85fb85b689440c (MD5) Previous issue date: 2009-07-24 / Brazil is the second world largest cassava producer and the largest South American producer. The aleyrodids, commonly known as whiteflies, are pests that affect cassava crops and might induce economic losses, due to high infestation levels. The objective of this work was to survey the whitefly species that occur feeding on cassava (Manihot esculenta Crantz) crops in Rio de Janeiro State, and to evaluate their population levels and the influence of the climate aspects on these levels as well. Sampling of leaves were realised fortnightly from April to December 2007 during visits to three commercial cassava farmlands located in the municipalities of Rio de Janeiro, Itagua? and Japeri. The samples were collected and analysed under optical microscopy, in laboratory, applying a presence-absence methodology. The species identification was realised according to morphological characters of the 4th instar nymphs. Among the 16 aleyrodid species described feeding on cassava in the Neotropical region, Aleurothrixus aepim (G?ldi, 1886) and Bemisia tuberculata Bondar, 1923 were identified occurring in the State. B. tuberculata is reported for the first time feeding on cassava crops in Rio de Janeiro State. Population peaks of A. aepim occurred from May to June and in December 2007 in all the farmlands analysed. B. tuberculata population levels got peaks in different periods, depending on the farmland analysed. Low temperatures registered in July 2007 suggest being the reason of the population density reduction of both species. / O Brasil ? o segundo maior produtor mundial de mandioca e o maior produtor do continente sul americano. Os aleirod?deos, insetos comumente conhecidos como moscas-brancas, s?o pragas que acometem esta cultura e podem causar preju?zos econ?micos, em decorr?ncia de altas infesta??es. O objetivo deste trabalho foi levantar as esp?cies de moscas-brancas que ocorrem na cultura da mandioca (Manihot esculenta Crantz) no Estado do Rio de Janeiro, bem como analisar sua flutua??o populacional e a influ?ncia de fatores clim?ticos sobre seus n?veis populacionais. Foram realizadas amostragens de folhas, quinzenalmente, no per?odo de abril a dezembro de 2007, em lavouras comerciais localizadas nos munic?pios do Rio de Janeiro, Itagua? e Japeri. As amostras foram coletadas e analisadas sob microscopia ?ptica em laborat?rio, aplicando-se uma metodologia baseada em presen?a/aus?ncia. A identifica??o das esp?cies foi feita por caracteres morfol?gicos das ninfas de 4.? instar. Das 16 esp?cies de aleirod?deos descritas na regi?o Neotropical em plantas de mandioca, foram identificadas ocorrendo no estado, Aleurothrixus aepim (G?ldi, 1886) e Bemisia tuberculata Bondar, 1923. ? registrada pela primeira vez a ocorr?ncia de B. tuberculata Bondar em mandiocais no estado do Rio de Janeiro. Picos populacionais de A. aepim ocorreram no per?odo de maio a junho e em dezembro de 2007 em todos os locais pesquisados. B. tuberculata teve picos em per?odos diferentes, conforme o local pesquisado. Temperaturas baixas ocorridas em julho de 2007 sugerem ser a causa da redu??o da densidade populacional de ambas as esp?cies.