Ph.D. THESIS TITLE Nutritional Biology of Synanthropic Mites (Acari: Acaridida) ABSTRACT Several attempts to describe the nutritional biology of acaridid mites were undertaken, however full understanding of these processes remains incomplete. The objective of this Ph.D. thesis was to expand our knowledge concerning digestive physiology of stored product and house dust mites and to apply this knowledge to their nutritional biology. The research approach adopted in this Ph.D. thesis includes in vitro characterization of enzymatic activity in whole mite extracts (WME) and spent growth medium extracts (SGME), evaluation of the enzyme activities with respect to the gut physiological pH, enzyme inhibition experiments, in vivo localization of enzyme activities in the mite gut, determination of effects of nutrient or antifeedant additives in experimental diets on mite population growth and determination of the feeding preferences of synanthropic mites as assessed by in vitro and in vivo analyses. The gut contents of twelve species of synanthropic acaridid mites were determined to be within a pH range of 4 to 7 and showed a pH gradient from the anterior to the posterior midgut. The pH in digestive tract of synanthropic acaridid mites corresponds to the activity of proteases, α-glucosidases, α-amylases and...

Biogas is an environmentally friendly energy source with great importance for sustainable development. The purpose of this study is to determine the feasibility of setting up a biogas plant at the student housing area planned at Albano in Stockholm. The possibility of attaining self-sustainability in Albano is also investigated. After compiling the processes for converting household waste into biogas through a literature study, a quantitative feasibility study of setting up a biogas plant is carried out. The usable amount of household waste is determined through an empirical study. Investment costs of comparable biogas production facilities are obtained from companies working in the biogas field. The producible biogas amount and rates of conversion from biogas to heat/electricity are derived from existing data in similar studies. The energy demand is calculated based on existing data from housing companies and authorities. Four possible scenarios are created to study all the possible outcomes of establishing a biogas plant. The maximum producible biogas is determined to be 12.199 m3/year. The total energy demand in Albano is determined to be 2.931MWh/year, of which 2,4 TWh/year is heat and 531 MWh/year is electricity. This amount is not sufficient to meet the specific requirements. Recommendations for Albano were made accordingly. / Biogas betraktas som ett en miljövänlig energikälla med stor betydelse för det kommande arbetet mot en mer hållbar utveckling. Denna rapport avser behandla möjligheten till ett upprättande av en biogasanläggning vid ett planerat studentboende vid Albano, Stockholm. Vidare kommer även möjligheterna till ett mer självständigt hållbart arbete i området behandlas. Som en del i rapporten kommer en analys av omvandlingen från hushållsavfall till biogas utföras i form av en kvantitativ studie med fokus på en förstudie för upprättande av biogasanläggning. Mängden hushållsavfall fås genom en empirisk studie av författaren. Den andel energi som beräknas kunna produceras fås genom befintlig data från utförda studier på Albano. Utifrån detta fastställs energibehovet utifrån givna rekommendationer och uppskattningar utförda av företag och myndigheter. Fyra möjliga utfall är definierade i syfte att kartlägga de möjliga utfallen för biogasanläggningen. Den maximala andel producerad biogas uppgår till 12.199 m3/år. Den totala energibehov i Albano bestämdes till 2.931 MWh/år, varav värmebehovet är 2,4 TWh/år och elbehovet är 531 MWh/år. Den maximala andel producerad biogas kan användas för att tillverka 48,8 MWh/år värme och 24,4 MWh/år elektricitet. Denna mängd är inte tillräcklig för att tillgodose de bestämda kraven. Utifrån detta har olika förslag utformats.

The cerebellum is well-known for its ability to integrate diverse internal and external stimuli, forming associations which serve as a basis for the construction of predictive models. Though traditionally studied in the context of motor learning, mounting evidence indicates that cerebellar learning may play an analogous role in many non-motor functions, including autonomic regulation and reward processing. Using combinatorial approaches of neuroanatomical tracing, electrophysiology, and behavioral studies, we investigated whether cerebellar learning extends to the domains of feeding behavior and gastrointestinal regulation, wherein prediction is critical for optimally timing food seeking and digestion in order to maximize nutrient acquisition while minimizing risk and energetic cost. Our results show direct anatomical connections between the cerebellum and known feeding centers, including the lateral hypothalamus, nucleus of the solitary tract, and parvocellular reticular nucleus. In addition, a localized area of the rostral cerebellum was found to respond to gastric distension and drive gastric emptying. Finally, we show that cerebellar activation drives robust, short-latency ingestive responses in awake, sated mice. Collectively, our results implicate the specific, interconnected cerebellar regions of Lobules II & III and the rostral fastigial nucleus, which we believe may represent one or more microzones devoted to integrating pre-and post-ingestive signals for control of feeding and digestion.

An investigation was carried out to study whether long-chain fatty acids (LCFAs) have an effect on digestion of waste sludge under anaerobic conditions. Four different kinds of LCFAs were used in this study. The 18 carbon series with 0, 1, 2 and 3 double bonds were studied to evaluate the degree of saturation on fatty acid degradation. Due to their molecular structure, unsaturated LCFAs are more soluble than saturated LCFAs. Oleic, linoleic, linolenic acid with an ascending number of double bonds were tested as representatives for three different degrees of saturation. In addition, stearic acid, a saturated fatty acid was also tested. LCFAs were added to sewage sludge at concentrations ranging from 5% to 20% on a weight basis and the pH, solids reduction and COD reduction were determined. The results suggested that in addition to degrading in the digesters, all unsaturated acids contributed additional solids removal, compared to the control group. In contrast, stearic acid did not affect the solids removal. The COD reduction was similar to solids reduction in that additional COD was destroyed when unsaturated LCFAs were added to the sludge. The mechanism for additional solids reduction is not known. / Master of Science

The Induced Bed Reactor (IBR) was developed at Utah State University to apply high-rate anaerobic digestion techniques to high solids content substrates. This technology has been successfully implemented at full-scale multiple installations in the United States and Canada as a waste treatment and energy production technology, but the physical processes necessary to further optimize the system were not well understood. Bench scale IBRs were operated as anaerobic digesters at 35°, 45°, and 55° C under three organic loading rates and three corresponding hydraulic retention times. Reactor performance was monitored at steady state for residence time distribution and substrate reduction. The results show that the IBR behaves as a retained biomass reactor with fluid mixing that most closely approximates Completely Stirred Tank Reactor (CSTR) behavior when operated under the study conditions. A compartment real CSTR model, incorporating elements of dead zone and bypass flow, appears to be the most appropriate representation of the data. Mixing is likely due to a combination of energy inputs from thermal gradients induced by heat flux through the reactors and reactor and shear rates induced by gas evolution in the sludge bed.

No description available.

The overall aim of this study was to provide new and updated information about subterranean termite morphology and digestive physiology. Scanning electron microscopy was used to obtain high resolution images of morphological features of the termite cuticle not discernable by light microscopy. In addition, digital scanning electron micrographs clearly show the appearance of wood particles recovered from the termite digestive tract at different stages of digestion. The ability of termites to obtain and conserve nitrogen in their diet was demonstrated by establishing whether the insect can digest chitin, as well as from determination of levels of soluble proteins and uric acid in the feces. Finally, a technique for determining the efficiency of cellulosic food digestion was tested. Scanning electron microscope images of alate abdomens showed similar appearance of cuticular structures between two subterranean termite species. In addition, the high magnification of electron microscopy allowed for identification of a pair of cuticular structures in the vicinity of the female genitalia that had not been reported from previous light microscope studies. Scanning electron microscopy also revealed the appearance of wood undergoing degradation as it traverses worker termite digestive tract, showing detail not possible from light microscope images. Adult workers of a native termite species were found to produce the enzymes needed to digest chitin, a nitrogen containing polysaccharide ingested by termites. Specific activity of chitinase was subsequently determined to establish the efficiency of chitin digestion for the termite species tested. As termites are coprophagous, significant levels of proteins measured from the feces demonstrated an additional potential dietary nitrogen source. Uric acid was generally found to be absent from termite feces, however. Digestive efficiency of wood cellulose could not be determined for Formosan termites due to the inability to obtain sufficient feces for quantification of undigested food contained in it. Previous studies of Formosan termite digestive efficiency did not address difficulty in obtaining feces for assay. Therefore, interpretation of data from earlier studies of Formosan termite digestive efficiency should be viewed with caution.

Nitrogen pollution in the environment creates challenging problems globally and locally and can be effectively controlled by a significant reduction in nitrogen release into the natural water system. In addition, nutrients in high-strength wastewater can be recovered as valuable resources such as different types of ammonium solutions for industrial and agricultural utilizations. Selective ammonia separation from high-strength wastewater can be achieved by bipolar membrane electrodialysis (BMED), a relatively new ion exchange technology. A series of 8 bench-scale BMED experiments with bipolar membranes and cation exchange membranes were performed under various voltage applications. Ammonia in the wastewater was rapidly separated and recovered as a high purity ammonium hydroxide solution. BMED operation for 30 minutes at 5.0 V per cell pair was found to be ideal for high purity ammonium hydroxide production and low electrical energy consumption. Additionally, effective organic fouling control and low energy consumption were achieved. The experiments showed a decrease in the feed pH making it ideal for applications in solid-state anaerobic digestion with leachate recirculation. The application of leachate recirculation in solid-state anaerobic digestion (SSAD) has proven effective for mobilizing nutrients and diluting toxic byproducts to enhance biogas production. The leachate after recirculation contains accumulated ammonia and an increased pH and requires water and chemicals for dilution and pH adjustment prior to recirculation. The data from the experiments were used to construct a numerical model for a hypothetical lab-scale and pilot-scale bipolar membrane electrodialysis and solid-state anaerobic digestion with leachate recirculation (BMED-SSAD) system. A final ammonia concentration of less than 2000 mg-N/L in the reactor was found to be achievable by lab-scale (6 mA/cm2) and pilot-scale (12 mA/cm2) BMED-SSAD and low electric energy consumption. The results suggest that BMED is an attractive solution for ammonia separation from high-strength wastewater. / Thesis / Master of Applied Science (MASc)

Ionic and osmotic homeostasis, and the intricately linked mechanisms of acid-base balance are critical for the survival of fish. To date, the role of the gastrointestinal (GI) tract in these processes has received only limited study, and our knowledge has been gained almost exclusively through studies conducted in fasting animals. The impacts of feeding and digestion, ubiquitous processes in the natural environment, are likely to be significant but have been overlooked. The current thesis addressed these shortfalls in our current understanding. Research focused on the rainbow trout (Oncorhynchus mykiss), a euryhaline species capable of withstanding the opposing challenges of life in seawater (diffusive influx of ions and loss of water) and freshwater (diffusive loss of ions and gain of water), and concentrated on its physiological response to ingestion of a meal of commercial, dry trout food, containing concentrated salts and little water. The net absorption and secretion of ions and water was tracked in each section of the GI tract of the rainbow trout over a detailed time course using an experimental diet that contained a simple inert marker, in the presence of external freshwater or seawater. Additionally, changes in overall blood chemistry were investigated to examine changes in osmotic, ionic and acid-base regulation during digestion. Feeding in freshwater resulted in the loss of endogenous water to the GI tract during digestion. Additionally, the meal provided much needed ions to balance those lost by diffusion; indeed all of the ingested ions were assimilated along the GI tract except for sodium which was absorbed in the stomach, but secreted in the intestine such that overall sodium balance was close to zero. Feeding also created a metabolic base load (an increase in the concentration of base, or HC03- due HCl secretion into the stomach lumen) that alkalinized the blood (i.e. caused a rise in pH), a phenomenon known as an alkaline tide. The base load was subsequently removed from the blood through increasedexcretion of base to the water via the gills. In seawater, the commercial diet again provided an avenue for water loss. This was potentially deleterious to an organism already suffering from diffusive water loss to the environment. Ion absorption from the diet was negligible, except for potassium and calcium, which were readily assimilated. As in freshwater, digestion resulted in an alkaline tide, however the mechanism of acid-base homeostasis differed with the excess base likely being excreted into the intestine. In contrast to freshwater fish, the gills took up additional base from the external environment, prolonging the acid-base disturbance in seawater fish. Overall, feeding was a dynamic process with far reaching systemic physiological effects. The research described highlighted intimate interactions between the processes of feeding and digestion and ion, water and acid-base homeostasis, and elucidated mechanisms that enable fish to inhabit a wide range of environments. / Thesis / Doctor of Philosophy (PhD)

No description available.