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  • About
  • The Global ETD Search service is a free service for researchers to find electronic theses and dissertations. This service is provided by the Networked Digital Library of Theses and Dissertations.
    Our metadata is collected from universities around the world. If you manage a university/consortium/country archive and want to be added, details can be found on the NDLTD website.
11

Nitretação por plasma contínuo e pulsado dos aços DIN X50 CrVMo 51 e DIN 34 CrAlNi7 / Continuous and pulsed plasma nitriding of the steels DIN X50 CrVMo 51 and DIN 34 CrAlNi 7

Nery Eugenio Chavez Bermudez 16 September 1999 (has links)
A nitretação por plasma, ao aumentar as durezas superficiais dos aços, amplia sua faixa de utilização, bem como a vida útil dos componentes produzidos com os mesmos. A disponibilidade de dados técnicos relativos ao tratamento de nitretação bem como ao comportamento das camadas obtidas, frente ao desgaste, são fundamentais à aplicação correta desse tratamento. No presente trabalho foram avaliadas as resistências ao desgaste abrasivo, por meio de ensaios do tipo pino-disco, dos aços DIN X50 CrVMo 51 (~AISI H 13) e DIN 34 CrAlNi 7 (nitralloy) nitretados por plasma em condições variáveis de tempo, temperatura, tipo de corrente e frequência de pulso. As camadas obtidas avaliadas por meio de ensaios de microdureza,metalográficos e as fases presentes foram determinadas por meio de difração de raios-X. Os comportamentos quanto ao desgaste foram relacionados com esses resultados obtidos. No caso do AISI H 13 foi também estudada a possibilidade da realização do segundo revenido simultaneamente com a nitretação. Esse aço apresentou características de resistência ao desgaste superiores às do aço Nitralloy. O tratamento em que se utilizou o duplo revenido anteriormente à nitretação foi menos efetivo do que o realizado simultaneamente com a mesma. A melhor condição de resistência ao desgaste ocorreu no caso do aço AISI H13 nitretado durante 3 h a 500ºC com corrente pulsada de 10 kHz. A resistência declinou com a diminuição da frequência do pulso. / The plasma nitriding, increasing the surface hardness of the steels, enlarges their use, as well as the useful life of the components produced with them. The readiness of relative technical data of the nitriding treatment as well as to the behavior of the obtained layers, with respect to the wear, are fundamental to the correct application of that treatment. In the present work the resistances of the abrasive wear they were evaluated, by means of the pin-on-disk test, of the steels DIN X50 CrVMo 51 (~AISI H13) and DIN 34 CrAINi 7 (Nitralloy) plasma nitrided in variable conditions of time, temperature, electrical current type and pulse frequency. The obtained layers were evaluated by means of microhardness and metalographic tests and the present phases were determined by means of R-X difraction. The behaviors with regards to the wear were related to those obtained results. In the case of AISI H13 steel was also studied the possibility of the simultaneously accomplishment of the second tempering with the nitriding. That steel presented resistance characteristics to the wear superiors to the on of the Nitralloy steel. The treatment in that the double tempering was used previously to the nitriding it was less effective than accomplished it simultaneously with the same. The best resistance condition to the wear happened in the case of the nitrided AISI H13 steel during 3:00 hours at 500°C with pulsed current of 10 kHz. The resistance decreased in a direct relation with the frequency of the pulse.
12

Hoje n??s dois, amanh?? n??s tr??s: din??mica conjugal durante a gravidez

Calazant, M??nica de Alcantara Sabino 07 December 2011 (has links)
Submitted by Sara Ribeiro (sara.ribeiro@ucb.br) on 2017-06-19T19:10:58Z No. of bitstreams: 1 MonicadeAlcantaraSabinoCalazantDissertacaoParcial2011.pdf: 207897 bytes, checksum: fd4d423a891a3cb4bdf1045d2685e20f (MD5) / Approved for entry into archive by Sara Ribeiro (sara.ribeiro@ucb.br) on 2017-06-19T19:11:06Z (GMT) No. of bitstreams: 1 MonicadeAlcantaraSabinoCalazantDissertacaoParcial2011.pdf: 207897 bytes, checksum: fd4d423a891a3cb4bdf1045d2685e20f (MD5) / Made available in DSpace on 2017-06-19T19:11:07Z (GMT). No. of bitstreams: 1 MonicadeAlcantaraSabinoCalazantDissertacaoParcial2011.pdf: 207897 bytes, checksum: fd4d423a891a3cb4bdf1045d2685e20f (MD5) Previous issue date: 2011-12-07 / According to the authors of the family life cycle, the transition to parenthood is one of the most intense phases of changes in family development, as it requires the couple's ability to restructure to include a new family member, with new responsibilities and roles that require negotiations and usages. This study aimed to investigate the marital relationship, especially during pregnancy, marital seeking to understand the dynamics during pregnancy, changes during this period and the strategies adopted to cope with these changes, the perceptions of spouses as to emotional and sexual relationship in each trimester of pregnancy and the couple's expectations regarding the child's birth. The study favored the reference of qualitative research, conducting a longitudinal study, characterized by regular observations of a given behavior over time and particularly that covered the three trimesters of pregnancy the couple participant through case study and survey and analysis of data were based on theoretical systemic approach to family. Participated in the study a couple formed by the husband, 24, computer technician and Anna, 23, promoter, living in a rented house in a neighborhood to the west of Belo Horizonte/MG, with a monthly income of five minimum wages. The couple was married for 11 months and expecting her first child at the time the research was done. There were three meetings with the couple in the same residence, the first early in the fourth month of pregnancy, the second at the end of the sixth month and the third meeting when his wife was in the ninth month of pregnancy. The meetings were recorded by voice recorder and later transcribed the data. The instruments used for data collection were three scripts for semi-structured, one for every moment of pregnancy, family genogram and road map for making glue. The data were submitted to content analysis showed that: the married dynamics during pregnancy undergoes the inevitable changes, sometimes uniting the couple around the pregnancy, sometimes away from him because of the disagreements that arise, there were complaints by the husband over the decrease in sex while his wife complained about his comments about her body, the couple studied showed a strong affective involvement, affective managed to organize and financially, and organize household routines to resolve conflicts, their expectations have been modified to every moment and in accordance with the development of pregnancy. The results also suggest that each member of the couple realizes the transition from an individual, from the moment the news of the pregnancy was not planned, we found individual differences in dealing with pregnancy, Anna was a wonderful surprise when Igor was resistant and had an acceptance gradually. During the pregnancy we also realize that women are more focused on the feelings, care, work, pain, etc.. While the man is concerned about the family's survival, protection, is a structure so that he can keep his family. / Segundo os autores do Ciclo de Vida Familiar, a transi????o para a parentalidade ?? uma das fases de mais intensas mudan??as no desenvolvimento da fam??lia, uma vez que requer do casal a capacidade de se reestruturar para incluir um novo membro na fam??lia, com novas responsabilidades e pap??is que demandam negocia????es e readapta????es. O presente estudo teve como objetivo investigar a rela????o conjugal, especificamente no per??odo gestacional, buscando compreender a din??mica conjugal durante a gravidez, as mudan??as ocorridas nesse per??odo e as estrat??gias adotadas para lidar com tais mudan??as, as percep????es dos c??njuges quanto ao relacionamento afetivo e sexual em cada trimestre de gravidez, bem como as expectativas do casal em rela????o ao nascimento da crian??a. O estudo privilegiou o referencial da pesquisa qualitativa, realizando um estudo longitudinal, caracterizado por observa????es regulares de um dado comportamento ao longo do tempo e que cobriu particularmente os tr??s trimestres de gesta????o do casal participante por meio de estudo de caso, e o levantamento e a an??lise dos dados tiveram como base te??rica a abordagem sist??mica da fam??lia. Participou do estudo um casal formado pelo esposo, 24 anos, t??cnico em inform??tica e Anna, 23, promotora de eventos, residentes em uma casa alugada em um bairro da zona oeste de Belo Horizonte/MG, com uma renda mensal de cinco sal??rios m??nimos. O casal estava casado h?? 11 meses e esperava seu primeiro filho no momento em que a pesquisa foi feita. Foram realizados tr??s encontros com o casal na resid??ncia do mesmo, sendo o primeiro no in??cio do quarto m??s de gesta????o, o segundo no final do sexto m??s e o terceiro encontro quando a esposa estava no nono m??s de gravidez. Os encontros foram gravados por gravador de voz e os dados posteriormente transcritos. Os instrumentos utilizados para levantamento dos dados foram: tr??s roteiros de entrevista semi-estruturados, um para cada momento da gesta????o, genograma familiar e roteiro para elabora????o da colagem. Os dados, submetidos ?? an??lise de conte??do, mostraram que: a din??mica conjugal durante a gravidez passa por mudan??as inevit??veis, ora unindo o casal em torno da gesta????o, ora distanciando-o em virtude das discord??ncias que surgem; houve queixas por parte do marido sobre a diminui????o das rela????es sexuais, enquanto a esposa reclamou dos coment??rios dele sobre seu corpo; o casal estudado apresentou um forte envolvimento afetivo, conseguiu se organizar afetiva e financeiramente, al??m de organizar as rotinas dom??sticas para resolver os conflitos; suas expectativas foram sendo modificadas a cada momento e de acordo com o desenvolvimento da gesta????o. Os resultados tamb??m sugerem que cada membro do casal percebe a transi????o de forma individual: desde o momento da not??cia da gravidez, que n??o foi planejada, encontramos diferen??as individuais na forma de lidar com a gesta????o: para Anna foi uma supresa maravilhosa enquanto Igor ficou resistente e teve uma aceita????o de forma gradual. No decorrer da gesta????o tamb??m percebemos que a mulher est?? mais voltada para os sentimentos, cuidados, tarefas, dores, etc., enquanto o homem preocupa-se com o sustento da fam??lia, a prote????o, ou seja uma estrutura para que ele possa manter a sua fam??lia.
13

Processo grupal na forma??o de coordenadores de grupo

Scola, Lourdes Sgarabotto 16 January 2013 (has links)
Made available in DSpace on 2015-04-14T13:22:10Z (GMT). No. of bitstreams: 1 447007.pdf: 235046 bytes, checksum: 368d92e627cb46a0cbe71bbb5d59d899 (MD5) Previous issue date: 2013-01-16 / This dissertation consists of two parts and aims to increase the knowledge of groups from the analysis of the group process. In the first part - Group Process in small groups - group concepts are presented, of the group process, theoretical operators, according to traditional and current authors for the theme have proposed and that influence the study and practice of group work currently. To discuss the group process aboard six aspects - openness, learning, resistance to change, interpersonal feedback, links reconfiguration, coordination - that are articulated with the principles of complex systemic thinking. In the second part - Group process in a training group of group coordinators - the results of the empirical research are presented, that generally aimed to understand how the group process occurred in a group of a course that trains students for careers coordinating groups. This is a qualitative study, transverse, composed by documentary research reports written by participants, as well as the field diary of one of the course coordinators. For data understanding, it was used Textual Analysis of Discourse strategy. Among the main results, stands out: (a) the opening as the first more expressive movement in the group process, followed by the resistance to change; (b) the interchangeable presence of these two categories on a frequent way, on a movement of surface/deepness; (c) the opening favored the appreciation of the individual learning and of the group process experienced: the group became a learning field. It s concluded that the process experienced by the group was of opening, resistance to change and learning, and the interpersonal feedback was the thread that promoted the movement, the overcoming of the challenges and favored the achievement of group goals / Esta disserta??o ? composta de duas partes e objetiva ampliar o conhecimento dos grupos a partir da an?lise do processo grupal. Na primeira parte - Processo grupal nos pequenos grupos - s?o apresentados conceitos de grupo, do processo grupal, operadores te?ricos, segundo autores tradicionais e atuais do tema propuseram e que influenciam o estudo e a pr?tica do trabalho com grupos atualmente. Para discutir o processo grupal s?o abordados seis aspectos abertura, aprendizado, resist?ncia ? mudan?a, feedback interpessoal, reconfigura??o de v?nculos, coordena??o articulados com os princ?pios do pensamento sist?mico complexo. Na segunda parte - Processo grupal em um grupo de forma??o de coordenadores - s?o apresentados os resultados da pesquisa emp?rica, cujo objetivo geral foi o de compreender como ocorreu o processo grupal num grupo do curso de capacita??o para coordenadores de grupos. Trata-se de um estudo qualitativo, transversal, composto por uma pesquisa documental dos relat?rios escritos pelos participantes, bem como do di?rio de campo de uma das coordenadoras. Para compreens?o dos dados foi utilizada a estrat?gia da An?lise Textual Discursiva. Entre os principais resultados destacam-se: (a) a abertura como primeiro movimento mais expressivo no processo grupal, seguido da resist?ncia ? mudan?a; (b) a presen?a intercalada dessas duas categorias de forma constante, num movimento de superf?cie/profundidade; (c) a abertura facilitou a valoriza??o do aprendizado individual e do processo grupal vivido: o grupo tornou-se campo de aprendizagem. Conclui-se que o processo vivenciado pelo grupo foi de abertura, resist?ncia ? mudan?a e aprendizado, sendo que o feedback interpessoal foi impulsionador do movimento, da supera??o das dificuldades e favoreceu o alcance dos objetivos grupais.
14

Beitrag zur Ermittlung der Kerbwirkung an Zahnwellen mit freiem und gebundenem Auslauf

Daryusi, Ali 28 April 2009 (has links) (PDF)
Durch die zunehmende technologische Entwicklung des Getriebe-, Gelenkwellen-, Werkzeugmaschinen-, Kraftfahrzeug-, sowie Landmaschinenbaus steigen die zu übertragenden Leistungen und Drehmomente enorm. Dies führt zu einem wachsenden Bedarf an formschlüssigen Profilwellenverbindungen und deren erhöhter Lebensdauer und Genauigkeit. Hierbei bilden die Zahnwellenverbindungen (ZWVen) mit Evolventenflanken nach DIN 5480 /N1/ den Regelfall für eine Vielzahl der Anwendung. Abhängig von Festigkeitsüberlegungen, Herstellungsverfahren und Platzbedarf treten in der Praxis nahezu ausschließlich die folgenden zwei Grundtypen auf. Es handelt sich dabei zum Ersten um die Zahnwelle (ZW) mit freiem Auslauf.Die zweite Geometrievariante ist die Zahnwelle mit gebundenem Auslauf, die eine nach DIN 471 /N2/ genormte Sicherungsringnut (SRN) enthalten kann. Zahnwellenverbindungen dienen zur Übertragung großer, wechselnder und stoßartiger Drehmomente ohne zusätzliches Verbindungselement durch die Profilierung der Welle und Nabe. Axiale Verschiebbarkeit unter Last, Profilverschiebungsmöglichkeit, einfache Montage und Demontage sowie die Herstellung mit hochleistungsfähigen umformenden und spanenden Massenfertigungsverfahren, die die Herstellungskosten verhältnismäßig niedrig halten, sind technisch bedeutsame Eigenschaften, die zum ansteigenden Einsatz von ZWVen führen (z.B. /N1/, /Vil84/, /Koh86/ und /Wes96/). Starke Kerbwirkung und erhebliche Überdimensionierung benachbarter Gestaltungszonen sind die wesentlichen Schwachpunkte der Profilverbindungen. Eine große Anzahl (ca. 80 %) von Ausfällen im Maschinenbau ist auf Schäden an Achsen und Wellen infolge konstruktiv bedingter Kerben zurückzuführen (z.B. /N3/ und /Hai89/). Speziell im Bereich der hochbeanspruchten Profilwellen-Verbindungen kommt es auf Grund der starken Querschnittsveränderungen und der häufig angewandten Ausläufe und Formelemente, z. B. Zahn- und Keilwellen zu Kerbwirkungen, die erhebliche örtliche Spannungskonzentrationen sowohl im Zahnfußbereich und Zahnlückenauslauf als auch im Bereich der Verbindung selbst verursachen. Diese Beanspruchungskonzentrationen sind fast in der Hälfte aller Zahnwellenbrüche die häufigste Ursache für Dauerbrüche (Ermüdungs- bzw. Schwingungsbrüche) und für Schäden (bleibende Verformung, Anriss, Gewaltbruch) infolge Maximalbelastung. Hier trifft die Lastüberhöhung am Welle-Nabe-Verbindungsrand mit dem Steifigkeitssprung des Verzahnungsendes auf der Welle zusammen /Die93/. Die erwähnten Schadensfälle belegen, dass der heutige Kenntnisstand über eine beanspruchungsgerechte Auslegung von Zahnwellen noch recht lückenhaft ist. Deshalb sind neue Erkenntnisse über Form- bzw. Kerbwirkungszahlen bei Einzel- und Mehrfachkerben von scharf und weniger scharf gekerbten Zahnwellen mit Auslauf für eine treffsichere Festigkeitsberechnung erforderlich und stellen damit die Hauptschwerpunkte dieser Arbeit dar. Das vorliegende Forschungsprojekt, welches sich erstmals mit der Ermittlung der Beanspruchungen in torsions-, und biegebelasteten Zahnwellen mit freiem und gebundenem Auslauf befasst, wurde im Rahmen der Forschungsvereinigung für Antriebstechnik e.V. (FVA) unter der Nummer T 467 und dem Forschungsthema „ Ermittlung der Kerbwirkung bei Profilwellen für die praktische Getriebeberechnung von Zahnwellen“ initiiert und untersucht.
15

Validierung der Solverimplementierung des hygrothermischen Simulationsprogramms Delphin

Sontag, Luisa, Nicolai, Andreas, Vogelsang, Stefan 26 November 2013 (has links) (PDF)
Das Simulationsprogramm Delphin ermöglicht die Berechnung des gekoppelten Wärme-, Feuchte-, Luft- und Stofftransports in kapillarporösen Materialien. Die Simulation verwendet ein numerisches Lösungsverfahren für die Differentialgleichungen welche die Transportprozesse beschreiben. Zur Kontrolle der numerischen Fehler sowie der korrekten Implementierung der physikalischen Gleichungen werden Validierungsrechnungen durchgeführt. Dafür werden vordefinierte Testfälle eingegeben, gerechnet und mit Referenzlösungen bzw. den Ergebnissen anderer Simulationsprogramme verglichen. In diesem Artikel werden die Ergebnisse der Validierung der Delphin Versionen 5.6, 5.8, 6.0 und 6.1 zusammengefasst. Es wurden folgende Testfälle gerechnet: HAMSTAD Benchmarks 1 bis 5, DIN EN ISO 10211 Fall 1 und 2, DIN EN 15026 und der Aufsaug-Trocknungs-Test. Die Validierung von Delphin erfolgte hinsichtlich des Wärme-, Feuchte- und Lufttransports bei ein- und zweidimensionalen Problemstellungen. Alle Programmversionen erfüllen die Anforderungen aller Testfälle.
16

Is Rational Mysticism Compatible with Feminism? A critical examination of Plotinus and Kashani

Cooper, Elisabeth Jane January 2006 (has links)
Plotinus (3rd century C.E.) and Afdal al-Din Kashani (12th century C.E.) each posit that the highest human goal is to become aware of the ultimate unity of reality. Both are rational mystics, and each describes a rigorous moral and intellectual training through which alone a human can achieve the goal. Seldom studied as a field in itself, rational mysticism offers a vision of philosophy that combines reason, intuition, virtuous practice, and mystical awareness. The relatively young discipline of feminist philosophy is both a response to what its practitioners see as male prejudice in past and present philosophical theories and an attempt to forge new, inclusive theories. Plato and Aristotle, among others, are called to account for their alleged contributions to the philosophically common representation of women as less rational than men, and to the development of philosophical and theological paradigms reflecting a male perspective. Since Plotinus and Kashani both owe much to Plato and Aristotle, including significant elements of how they conceptualise human nature and the nature of ultimate reality, it might be expected that they would incur the same criticisms. So far, however, little feminist attention as such has been paid to Plotinus and the rational mystics of the Islamic tradition, and almost none to Kashani. My examination of these two figures is an attempt to rectify this neglect. In addition, for the first time in a feminist historical critique of this kind, a diversity of feminist perspectives is taken into account. Thus, the question 'Is rational mysticism compatible with feminism?' will be seen to yield a somewhat different answer according to which group of feminists is in view. In offering a revisionist interpretation of Plotinus and Kashani, I aim first to establish which of their theses are consistent with feminist theses; second, to determine whether the consistency of theses is significantly affected, in Kashani's case, by the additional influence of Islamic religion; and third, to identify which group or sub-group of feminists could find in rational mysticism resources for reconstructive work in philosophy. I thereby aim to enrich the understanding of both rational mysticism and feminism.
17

Studies on the works of Abu Shama 599-665 A.H. (1203-1267)

Ahmad, M. H. M. January 1951 (has links)
No description available.
18

Major problems in social entrepreneurship: Development aid through the case study of Din-Daeng flat

Roca, Marta January 2019 (has links)
This thesis is about social entrepreneurship and its role in addressing social issues. Some of social entrepreneurship’s main features includes its focus on creating social value, achieving a sustainable solution to the problems it addresses, and empowering its recipients. These characteristics turn social entrepreneurship into a good driver of social change, a goal that could be comparable to development aid and NGOs objectives. However, the scientific research around social entrepreneurship is still in its early stages. This study intends to bridge a gap in the literature by comparing social entrepreneurship with development aid and NGOs using a case study of Din-Daeng flat in Bangkok. Participant-observation, in-depth interviews, and surveys are the major methods employed in this research. The results show that some social issues, like the one reflected in the Din-Daeng flats, would find a better solution through social entrepreneurship than through official development aid and NGOs. At the end, this study provides a social entrepreneurship plan that could be used to address Din-Daeng’s issue.
19

Validierung der Solverimplementierung des hygrothermischen Simulationsprogramms Delphin

Sontag, Luisa, Nicolai, Andreas, Vogelsang, Stefan 26 November 2013 (has links)
Das Simulationsprogramm Delphin ermöglicht die Berechnung des gekoppelten Wärme-, Feuchte-, Luft- und Stofftransports in kapillarporösen Materialien. Die Simulation verwendet ein numerisches Lösungsverfahren für die Differentialgleichungen welche die Transportprozesse beschreiben. Zur Kontrolle der numerischen Fehler sowie der korrekten Implementierung der physikalischen Gleichungen werden Validierungsrechnungen durchgeführt. Dafür werden vordefinierte Testfälle eingegeben, gerechnet und mit Referenzlösungen bzw. den Ergebnissen anderer Simulationsprogramme verglichen. In diesem Artikel werden die Ergebnisse der Validierung der Delphin Versionen 5.6, 5.8, 6.0 und 6.1 zusammengefasst. Es wurden folgende Testfälle gerechnet: HAMSTAD Benchmarks 1 bis 5, DIN EN ISO 10211 Fall 1 und 2, DIN EN 15026 und der Aufsaug-Trocknungs-Test. Die Validierung von Delphin erfolgte hinsichtlich des Wärme-, Feuchte- und Lufttransports bei ein- und zweidimensionalen Problemstellungen. Alle Programmversionen erfüllen die Anforderungen aller Testfälle.:1 Einleitung 1.1 Fehlerquellen bei numerischen Lösungsverfahren 1.2 Validierungssystematik 1.3 Beschreibung der Testfälle 2 Materialgenerierung 2.1 Konstante Speichereigenschaften 2.2 Konstante Transporteigenschaften 2.3 Feuchtespeicherung 2.4 Feuchtetransport 2.5 Wärmeleitung 2.6 Materialdatei 3 HAMSTAD Benchmark 1 3.1 Materialdaten 3.2 Klimadaten und Randbedingungen 3.3 Validierungsrechnung 4 HAMSTAD Benchmark 2 4.1 Materialdaten 4.2 Klimadaten und Randbedingungen 4.3 Validierungsrechnung 5 HAMSTAD Benchmark 3 5.1 Materialdaten 5.2 Klimadaten und Randbedingungen 5.3 Validierungsrechnung 6 HAMSTAD Benchmark 4 6.1 Materialdaten 6.2 Klimadaten und Randbedingungen 6.3 Validierungsrechnung 7 HAMSTAD Benchmark 5 7.1 Materialdaten 7.2 Klimadaten und Randbedingungen 7.3 Validierungsrechnung 8 DIN EN ISO 10211 Fall 1 8.1 Materialdaten 8.2 Klimadaten und Randbedingungen 8.3 Validierungsrechnung 9 DIN EN ISO 10211 Fall 2 9.1 Materialdaten 9.2 Klimadaten und Randbedingungen 9.3 Validierungsrechnung 10 DIN EN 15026 10.1 Materialdaten 10.2 Klimadaten und Randbedingungen 10.3 Validierungsrechnung 11 Aufsaug-Trocknungs-Testfall (Wetting&Drying) 11.1 Materialdaten 11.2 Klimadaten und Randbedingungen 11.3 Validierungsrechnung 12 Zusammenfassung
20

Predi??o da estrutura 3D de prote?nas mimetizando o ambiente riboss?mico

Borja, Carlos Eduardo Sequeiros 23 February 2017 (has links)
Submitted by Caroline Xavier (caroline.xavier@pucrs.br) on 2017-06-29T13:51:27Z No. of bitstreams: 1 DIS_CARLOS_EDUARDO_SEQUEIROS_BORJA_PARCIAL.pdf: 2369724 bytes, checksum: 0bcf4fe536f9f8fc084f08e3dc335db9 (MD5) / Approved for entry into archive by Caroline Xavier (caroline.xavier@pucrs.br) on 2017-06-29T13:51:38Z (GMT) No. of bitstreams: 1 DIS_CARLOS_EDUARDO_SEQUEIROS_BORJA_PARCIAL.pdf: 2369724 bytes, checksum: 0bcf4fe536f9f8fc084f08e3dc335db9 (MD5) / Made available in DSpace on 2017-06-29T13:51:46Z (GMT). No. of bitstreams: 1 DIS_CARLOS_EDUARDO_SEQUEIROS_BORJA_PARCIAL.pdf: 2369724 bytes, checksum: 0bcf4fe536f9f8fc084f08e3dc335db9 (MD5) Previous issue date: 2017-02-23 / Coordena??o de Aperfei?oamento de Pessoal de N?vel Superior - CAPES / Conselho Nacional de Pesquisa e Desenvolvimento Cient?fico e Tecnol?gico - CNPq / Protein structure prediction from just the amino acid sequence continues to be a major challenge in structural bioinformatics. If at all possible, prediction needs to be accurate and fast. In this project, it is proposed and tested the effects of cotranslation within an ideal ribosomal channel model in protein structure prediction using classical molecular dynamics and replica-exchange molecular dynamics simulations. An ideal ribosomal channel model was built, different translation speeds were used and compared the results to control simulations. Different translation speeds were tested to verify their influence on predictions, and the best results were observed at translation speeds between 80 and 200 ps. The quality of the predicted models were as low as 0.3 ? and 1.0 for the RMSDs and GDT-TS parameters, respectively, for simulations of just 50 ns. Overall, the use of this approach to protein structure prediction has successfully produced native and near-native structures in three of the four proteins investigated, thus reaching accuracy and speed as expected. As a conclusion, using cotranslation within an IRCM is a promising approach to predict native-like 3D structures of mini-proteins successfully. Improvements to the methodology should allow the prediction of 3D structures of larger proteins of biological and biomedical interest. / A predi??o de estrutura 3D de prote?nas partindo apenas da sequ?ncia de amino?cidos ainda ? um grande desafio em bioinform?tica estrutural. Apesar da dificuldade, a predi??o precisa de ser acurada e r?pida. Nesta disserta??o, prop?e-se e mesuram-se os efeitos da co-tradu??o e o uso de um modelo ideal de canal ribossomal na predi??o da estrutura 3D de prote?nas, fazendo uso de din?mica molecular cl?ssica e din?mica molecular com intercambio de r?plicas. O modelo do canal ribosomal constru?do foi testado com diferentes velocidades de tradu??o, e os resultados foram comparados com simula??es padr?o. Foram testadas diferentes velocidades de tradu??o para verificar sua influ?ncia nas predi??es, e as velocidades que apresentaram os melhores resultados ficaram na faixa de 80 at? 200 ps. A qualidade dos modelos preditos foram boas, apresentando valores de GDT-TS de 1,0, assim como 0,3 ? para RMSD para simula??es de apenas 50 ns. No geral, demostra-se que o uso desta abordagem na predi??o da estrutura de prote?nas, produz satisfatoriamente estruturas nativas ou perto da nativa em tr?s de quatro prote?nas testadas, atingindo assim a acur?cia e velocidade esperadas. Como conclus?o, o uso da co-tradu??o com um modelo do canal ribosomal ? uma abordagem promissora para a predi??o de estruturas de mini prote?nas perto da estrutura nativa. Melhoras na metodologia e no modelo permitir?o uma predi??o de estruturas 3D de prote?nas maiores de interesse biol?gico e biom?dico.

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