Since bone tissues grow in intimate contact with implant surfaces in vivo, there is a need to investigate how bone cells respond to mechanical loads adjacent to implants under well characterized loading conditions that stimulate the bone-implant surface. Thus, the objective of this study was to demonstrate an effective means for applying known, uniform, cyclic strain to cells growing on implant materials in vitro. A cell culture strain plate device was developed based on the application of the four-point bending principle. The device uses a small electric motor to drive belts attached to shafts which turn a set of cams. The cams are attached to pins which connect to a titanium plate which rests over arched supports. When deflected and depending on which set of cams are used, strains generated range from around 200 to 1000 ìstrain. UMR-106 osteoblast-like cells were cultured on the titanium plate, and the plate was deflected at three strain magnitudes at 1.5 Hz for durations of 4 and 24 hours. Strain gages recorded average maximum strain levels of 182 ± 3, 366 ± 9, and 984 ± 7µstrain. The strain device, with attached cells, was tested in an amiable bioenvironment. Results from strain gages indicated a uniform strain field existed within the center region of the plate and culture area. Cells in the test plates stained viable, exhibited similar morphology to controls, and were assayed for alkaline phosphatase (ALP) activity, total protein production, and calcium deposition. Results also indicated that stretched cells exhibited increases in proliferation, as well as changes in ALP activity vs. unstrained controls. Thus, the device was successful in distinguishing differences in cell response to mechanical perturbations and may be used to investigate how cells respond to strains at implant-bone interfaces.

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<p> The thesis summarizes the experimental and analytical results of studies on the behavior of two FRP-encased steel-concrete composite columns under axial loading. Composite columns have been conventionally constructed using steel and concrete. This study utilizes FRP in combination with steel and concrete to manufacture composite columns with enhanced behavior. The first type of column is a concrete-encased steel column wrapped with epoxy-saturated glass and carbon fiber reinforced polymer (GFRP and CFRP) sheets in the transverse direction. The second type of composite column utilizes a GFRP tube that surrounds a steel I section column, which is subsequently filled with concrete. </p> <p> To the best of the author's knowledge, columns comprising FRP, steel and concrete in the shape of the proposed composite systems has not been reported on in the literature. This study includes two major phases. In the first phase, behavior of stub columns is investigated where stability effects are ignored and failure is governed by the loss of cross-sectional strength. In the second phase, influence of stability on the behavior of the proposed composite columns is studied by testing specimens with various slenderness ratios. </p> <p> To investigate the cross-sectional strength, a total of nine short (500 mm in height) composite column specimens were constructed and tested under axial compression. Five specimens were wrapped with FRP sheets and the remaining four were constructed using a GFRP tube. Experimental results showed significant enhancement in the behavior of the composite columns which was achieved due to confinement and composite action between the constituent materials. The compressive strength of the confined concrete core in the composite specimens constructed using FRP sheets and GFRP tube increased by a factor of 2.4 and 1.8, respectively. An analytical model was developed to predict crosssectional behavior of the proposed composited column. </p> <p> With the primary objective of investigating the influence of slenderness on the behavior of the composite columns, ten additional column specimens, ranging between 1,000 mm and 3,000 mm in height, were tested. Five specimens were constructed using FRP sheets and five constructed using the GFRP tube technique. It was found that the compressive strength of the confined concrete core in the longest tubular composite specimen was reduced to approximately 60% of that of the corresponding short specimen. No confinement was achieved in the longest FRP wrapped composite column specimen. </p> <p> Three bare steel columns, ranging between 500 mm and 3,000 mm in height, were also tested to facilitate comparison with the composite columns in terms of increased axial capacity, as well as stiffness and energy dissipation characteristics of the columns. The compressive strength, elastic axial stiffness and ultimate axial strain of the bare steel columns increased by a factor of up to 10, 6 and 3, respectively, in the composite columns constructed utilizing the concrete-filled GFRP tube. These factors were reduced to 5 .2, 2.5 and 2.6, respectively, in the concrete-encased steel columns wrapped with FRP jackets. </p> <p> Finally, an analytical model was developed to establish the capacity curves for the proposed composite columns accounting for slenderness effects. A simple design equation to predict the compressive strength of the tubular composite columns was proposed based on the capacity curve generated from the analytical model. Compressive capacity of the composite columns predicted using the proposed design equation showed favorable agreement with the experimental results. </p> / Thesis / Doctor of Philosophy (PhD)

Nineteen elite (E) gymnasts (>15hours/week gymnastic training), 14 high recreation (HR) gymnasts (8-15hours/week), and 15 low recreation (LR) gymnasts (1-7. 9 hours/week) were investigated to determine the effects of varying volumes of gymnastic training on bone mineral density (BMD) in prepubescent girls. Two normoactive control groups were additionally investigated to determine whether there was a magnitude effect of mechanical loading on BMD: 16 controls (C) and 15 height-and weight-matched controls (M). The mother of each daughter was measured in order to control and investigate the familial component of bone mass. Areal bone mineral density at the left proximal femur, lumbar spine (LS), and whole body (WB) and % body fat were measured by dual energy x-ray absorptiometry (DXA), and volumetric BMD was measured at the distal radius by peripheral QCT (pQCT). DXA BMD measures were corrected for bone size and expressed as bone mineral apparent density (BMAD). The HR group was significantly younger (8.68 ± 0.844 y, mean± SD) than both the E (10.02 ± 0.776 y) and C (9.96 ± 0.898 y) groups. The C group was significantly heavier (38.88 ± 4.868 kg) than the E (27.15 ± 2.819 kg), HR. (25.44 ± 3.564 kg), LR (32.98 ± 5.786 kg), and M (26.95 ± 3.301 kg) groups. Additionally, the LR group was significantly heavier than all other groups, with the exception of the C group. Femoral neck (FN) BMD was only significantly different between the E (0.706 ± 0.051 g•cm⁻²) and LR (0.649 ± 0.069 g•cm⁻²) groups. FNBMAD was only greater in the E (0.232 ± 0.048 g•cm⁻³) group compared to the C (0.191 ± 0.052 g•cm⁻³) group. LSBMAD and WBBMAD were significantly greater in both E (0.233 ± 0.019 and 0.100 ± 0.008 g•cm⁻³) and HR (0.239 ± 0.038 and 0.100 ± 0.006 g•cm⁻³) groups when compared to the LR (0.212 ± 0.022 and 0.090 ± 0.008 g•cm⁻³) and C (0.219 ± 0.020 and 0.085 ± 0.004 g•cm⁻³) groups, respectively. Total radial and cortical radial BMD was greater in both E (360.50 ± 51.569 and 484.28 ± 70.179 mg•cm⁻³) and HR (373.10 ± 45.318 and 480.66 ± 46.720 mg•cm⁻³) groups compared to the C (296.61 ± 29.677 and 426.144 ± 37.652 mg•cm⁻³) and M (306.42 ± 24.430 and 414.571 ± 25.194 mg•cm⁻³) groups, respectively. Radial trabecular BMD was greater in both E (211. 19 ± 38. 202 mg•cm⁻³) and HR (212. 61 ± 44.299 mg•cm⁻³) groups compared to the LR (175.89 ± 29.191 mg•cm⁻³), C (162.68 ± 27.304 mg•cm⁻³), and M (171.05 ± 30.639 mg•cm⁻³) groups. There were no significant differences for any bone measure among the groups of mothers. Mother-daughter correlations were relatively weak, and often insignificant, for BMD measures (r = 0.10-0.37), but strong for radial morphometric measures (r = 0.43-0.55). Radial trabecular BMD (r = 0.37; p<0.01) was more significantly correlated with gymnastic training volume (hours/week) than radial cortical BMD (0.30; p<0.05). These results suggest that there is a volume of training effect on BMD and a magnitude effect of mechanical loading on BMD. It appears that trabecular bone at the distal radius may adapt more rapidly or be more sensitive than cortical BMD to the strains imposed by impact loading. Additionally, it appears that, during prepubescence in females, bone morphometric properties may be more genetically regulated than bone mineralization. / Thesis / Master of Science (MSc)

No / Closed-form expressions for the small axial deflection and stress distribution of axially loaded rubber blocks of elliptical cross-section, whose ends are bonded to rigid plates, are derived using a superposition approach. The governing equations and conditions are satisfied exactly, based upon the classical theory of elasticity. Easily calculable expressions are derived for the corresponding apparent Young¿s modulus and the modified apparent Young¿s modulus in forms analogous to those previously given for blocks of circular cross-section.

The research presented in this dissertation furthers the state of the art for reliability-based design of composite structures subjected to high cycle variable amplitude (spectrum) fatigue loads. The focus is on fatigue analyses for axially loaded fiber reinforced polymer (FRP) composites that contain a significant proportion of fibers in the loading direction and thus have fiber-direction dominated failure. The four papers presented in this dissertation describe the logical progression used to develop an improved reliability-based methodology for fatigue-critical design. Throughout the analysis extensive experimental fatigue data on several material systems was used to verify the assumptions and suggest the path forward. A comparison of 12 fatigue model approaches from the literature showed that a simple linear residual strength approach (Broutman and Sahu) provides an improvement in fatigue life prediction compared to the Palmgren-Miner rule, while more complex residual strength models did not consistently improve on Broutman and Sahu. Evaluation of the effect of load history randomness on fatigue life was made using experimental results for spectra in terms of the first order autocorrelation of the stress events. For approximately reversed Rayleigh distributed fatigue loading, load sequence was not critical in the material behavior. Based on observations of empirical data and evaluation of the micro-mechanics deterioration and failure phenomena of FRP composites under fatigue loading, a new residual strength model for the tension and compression under any load history was proposed. Then this model was implemented in a stochastic framework and a method was proposed to enable calculation of the load and resistance factor design (LRFD) parameters for realistic reliabilities with relatively few computations. The proposed approach has significant advantages over traditional lifetime-damage-sum-based reliability analysis and provides a significant step toward enabling more accurate reliability-based design with composite materials. / Ph. D.

The response of light-frame timber shear walls to lateral forces is the focus of the dissertation. The objective of this study was to obtain performance characteristics of shear walls with various aspect ratios and overturning restraint via experimental testing and analytical modeling. Presented are the test data of monotonic and cyclic tests on fifty-six light-frame timber shear walls with aspect ratios of 4:1, 2:1, 1:1, and 2:3. Overturning restraint conditions represent engineered construction and conventional construction practices. The walls representative of the engineered construction were attached to the base by means of tie-down anchors and shear bolts. As opposed to engineered construction, conventionally built walls were secured to the base by nails or shear bolts only. The specimens were tested in a horizontal position with oriented strandboard (OSB) sheathing on one side. To obtain conservative estimates, no dead load was applied in the wall plane during the tests. The nail-edge distance across the top and bottom plates varied from 10 mm (3/8 in.) to 19 mm (3/4 in.). Twelve walls were repaired after the initial tests and re-tested. A mechanics-based model was advanced to predict the racking resistance of conventional multi-panel shear walls using simple formulae. The deflections of engineered and conventional shear walls were predicted using the energy method combined with empirical formulae to account for load-deformation characteristics of sheathing-to-framing connections and overturning restraint. The proposed formulae were validated through comparison with test results obtained during this study. The results of the study serve to further development of a mechanics-based methodology for design of shear walls accounting for various wall configurations and boundary conditions. / Ph. D.

California has experienced an unusually dry past two decades punctuated by three intense multi-year droughts from 2007-2010, 2012-2015, and 2020-2022. A portion of the water lost during these two decades is due to intense groundwater overdraft of the Central Valley Aquifer. This groundwater overdraft has led to poroelastic compaction of the aquifer system and subsidence of the land surface. Water mass loss also causes elastic deformation of the solid Earth, an opposite and smaller amplitude response than the poroelastic deformation of aquifer systems. These mass changes can disturb the regional stress field, which may influence earthquake activity. Both the elastic and poroelastic deformation responses can be observed using satellite-based geodetic tools including Global Navigation Satellite System (GNSS) station displacements and Interferometric Synthetic Aperture Radar (InSAR). In this dissertation, I model aquifer-system compaction at depth using InSAR-based vertical land motion during the 2007-2010 drought and evaluate hazards related to Earth fissures, tensional cracks that form at the edges of subsidence zones. Next, I forward-calculate the predicted elastic deformation response to groundwater mass loss over the same period and calculate crustal stress change to evaluate what, if any, impact this has on seismicity in California. In addition to modeling deformation caused by water storage change, I also introduce a new method to jointly invert elastic vertical displacements at GNSS stations with water storage anomalies from the Gravity Recovery and Climate Experiment (GRACE) to solve for water storage changes from 2003-2016 over California. Finally, I expand on this joint inversion framework to include poroelastic deformation measured using InSAR over the Central Valley aquifer-system to solve for a change in water storage and groundwater storage over water years 2020-2021, the most recent drought period in California. / Doctor of Philosophy / Changes in the hydrologic system can have wide-reaching societal, geopolitical, economic, ecological, and agricultural impacts. Proper water management, particularly in places that have water scarcity concerns due to overuse, water pollution, or recurrent drought conditions, is essential to ensure this resource is available to future generations. Current projections of climate change scenarios point to more intense and frequent extreme hydroclimate events. With accelerating population growth in many urban centers across the world, measuring water storage changes has never been more important to ensure resiliency of our cities, energy sector, and agricultural systems. Furthermore, water storage changes deform the Earth, which may create or alter geophysical hazards such as subsidence, the development of Earth fissures, and seismicity. Today, a multitude of space-based geodetic tools allow us to monitor changes in the Earth system, including changes in terrestrial water content and associated deformation, with higher spatial and temporal resolution than ever before. These datasets have provided an unprecedented understanding of hydroclimatic hazards and have resolved constraints arising from sparse and infrequent in-situ measurements. Here, I use space-based geodetic tools and geophysical models to measure water storage fluctuations, deformation, and evaluate associated hazards in California, a region that has experienced an unprecedented nearly continuous two-decades long drought. In general, I find that 21st century droughts have caused significant water storage loss, especially groundwater storage loss, in California, which has exacerbated some geophysical hazards including land subsidence and Earth fissure hazards.