Drug facilitated assault (DFA) is an increasing problem in the UK. The crime often occurs through the surreptitious administration of a drug into a victims drink, rendering the victim unable to resist the assault. The detection of these drugs in a biological specimen from the victim is one of the most challenging facets of forensic chemistry. Drug concentrations can be very low, as often only a single dose is administered, and the pharmacodynamics of commonly employed drugs further hinders the testing process. The research presented in this work shows the development of several new assays for the detection of flunitrazepam, gamma-hydroxybutyrate (GHB) and ethyl glucuronide (EtG) in a variety of biological matrices. New methods of drug testing in blood and urine are demonstrated, as well as interesting developments in the field of hair testing. Using hair to detect drug exposure allows a much wider window of detection than the more traditional matrices of blood and urine. New methods are presented in this work using gas chromatography-tandem mass spectrometry (GCMS/MS) to detect drugs in hair. Validation data is presented along with the results of authentic DFA testing. All aspects of the drug testing procedure have been evaluated, from new extraction techniques utilising water instead of solvents, to novel clean up stages involving the unique combination of SFE and SPME. Several confirmation techniques are explored including single quadrupole, triple quadrupole and ion trap mass spectrometry. In addition to developing assays for DFA cases, the versatility of this type of analytical chemistry is explored in two population studies. The first study evaluates alcohol consumption between two groups; drugs users and non drug users in medico-legal cases. There is an anecdotal belief amongst drug clinic staff that alcohol use is lower in drugs users than it is in non drug users. This study presents the first scientific confirmation of this belief through EtG (an alcohol metabolite) testing in hair of the two groups. The second study investigates whether there is a correlation between EtG and cocaethylene (a metabolite of cocaine only produced in the presence of alcohol) in cocaine users. Results f this study suggest that there is no positive correlation between the two compounds. The research presented in this thesis aims to further the analytical science surrounding FA investigation and provide accurate, sensitive and reliable methodology for drug esting in blood, urine and hair.

The field of equine therapy (ET) in Canada and the USA encompasses a range of distinct approaches, such as equine-facilitated therapy, equine-assisted therapy, therapeutic riding, and hippotherapy. Due to issues like inconsistent terminology and lack of standardized practice manuals, there are gaps in the ET knowledge about how these approaches relate or differ from each other. This research reviewed 47 scholarly, peer-reviewed articles about ET approaches and applied thematic analysis to determine key themes that clarify key traits of each approach. Recognizing that children and youth are common participants in equine therapy, this research was motivated by the questions: What type of relational dynamics are modelled for children and youth in ET? How are these horse-human relationships portrayed? Which participants possess their own agency? Findings of this research provide insights about each approach of ET and highlight the therapeutic affects of interspecies relationships between humans and horses. Contradictory viewpoints about mutual agency between species are identified in language that described horses are active, intelligent subjects and/or passive, inanimate objects. This research provides insights about the different forms of ET, highlights important benefits and gaps, and invites the fields of Child and Youth Care and Animal-Assisted Therapy to critically reflect on the relational tensions of employing non-human animals in human therapy. / Graduate

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Anxiety can be advantageous in terms of survival and well-being, yet atypically high levels may be maladaptive and result in the clinical diagnosis of an anxiety disorder. Several risk factors have been implicated in the manifestation of clinical anxiety, including cognitive biases. In recent years, a plethora of research has emerged demonstrating that anxious adults exhibit biases of attention for threatening stimuli, especially that which is biologically relevant (e.g., facial expressions). Specific components of attentional bias have also been identified, namely facilitated engagement, impaired disengagement, and avoidance. However, the majority of studies have focused on the spatial domain of attention. Furthermore, the area is under-researched in children, despite research demonstrating that symptoms relating to clinical and non-clinical anxiety follow a stable course from childhood through to adolescence and adulthood. Consequently, the aim of this thesis was to investigate how anxiety affects children’s visual attention for emotive, particularly angry, faces. In order to provide a more comprehensive understanding, the current research involved examining the role of temporal and spatial attention utilising rapid serial visual presentation with the attentional blink, and the visual probe paradigm, respectively. The main hypothesis was that high state and/or trait anxiety would be associated with an attentional bias for angry, relative to positive or neutral faces in both the temporal and spatial domains. In relation to the temporal domain, key findings demonstrated that high levels of trait anxiety were associated with facilitated engagement towards both angry and neutral faces. It was further found that all children rapidly disengaged attention away from angry faces. Findings related to the processing of angry faces accorded with the main hypothesis stated in this thesis, as well as research and theory in the area. The finding that anxious children preferentially processed neutral faces in an attentional blink investigation was unexpected. This was argued to potentially reflect this stimulus type being interpreted as threatening. Key findings regarding the spatial domain were that high trait anxious children displayed an early covert bias of attention away from happy faces and a later, overt bias of attention away from angry faces. The finding that high trait anxiety was linked to an attentional bias away from happy faces in a visual probe task was also unexpected. This was argued to potentially reflect smiling faces being interpreted as signifying social dominance, thus resulting in the viewer experiencing feelings of subordination and becoming avoidant and/or submissive. To conclude, this thesis has enhanced current knowledge of attentional bias in both the temporal and spatial domains for emotive stimuli in anxious children. It has demonstrated that higher levels of trait anxiety moderate children’s allocation of attentional resources to different stimulus types, whether these are threatening, positive, or neutral. This has important implications for evaluating past research in adults and children, and for further developing theoretical models of attentional bias and anxiety. It also offers important clinical implications, since attending towards or away from specific stimuli may affect the development and maintenance of anxiety disorders. Recently, a treatment that aims to modify attentional bias in anxious individuals has begun to be developed. In light of the present findings, it may be necessary to review this treatment so that anxious children are re-trained in the specific biases of attention demonstrated here.

Text in English / Equine-Assisted Psychotherapy (EAP) is a form of Animal Assisted Therapy (AAT) used to treat individuals’ psychological problems. EAP is an interactive process in which a licensed mental health practitioner, a credentialed equine professional and suitable equines work together to address psychotherapy goals. Since the 1990s, research on EAP has grown rapidly in the United States of America (USA) and Europe, however research in the South African context is lacking. This descriptive and exploratory research study explored and described the experiences of licensed mental health practitioners and/or credentialed equine specialists who have included EAP within their practise by focusing on the role that equines play within the psychotherapeutic process. The knowledge of this study was derived from a Constructivistic epistemology. Constructivism argues that humans attain and build knowledge and meaning from their experiences. Participants were selected based on purposeful criterion sampling. Only licensed mental health practitioners or credentialed equine specialists who included EAP within their practise, registered at the Equine-Assisted Psychotherapy Institute of South Africa (EAPISA) or the Equine-Assisted Growth and Learning Association (EAGALA) were considered for participation. Semi-structured, face-to-face interviews were conducted with six participants. A post-interview follow-up email was used to gain additional feedback from each participant. Finally, data was analysed using thematic analysis. As EAP is a fairly new modality of therapy within South Africa, the contribution of this study is to add to the growing body of professional EAP literature. Thematic analysis identified seven main themes: shifting dynamics in the therapeutic relationship, setting the scene for storytelling, the equine as an intermediary and therapeutic tool, what the equine sees is what you get, instantaneous but lasting results, variations and similarities in approach and activities, the emotive motives of EAP practitioners. These themes are discussed in relation to the research findings. Findings of this study showed substantial agreement in what the equine brings to the psychotherapeutic session: unique equine attributes, opportunities for metaphorical learning, and relational aspects. The participants demonstrated a consensus in the belief that EAP can be beneficial to a large spectrum of psychotherapeutic populations in South Africa. A recommendation for future research is for larger, international studies that explore the viewpoints of psychotherapists who practice equine therapies in other countries in order to expand the knowledge base and address the competency and standards discussion in the EAP field. / Psychology / M.A. (Psychology)

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