The glucose transport protein, GLUT1, is highly expressed in rapidly proliferating cells, including cancer cells, while decreased GLUT1 levels are found in diseases such as GLUT1 deficiency syndrome and Alzheimer’s. There is increased interest in developing GLUT1 inhibitors as novel anticancer therapeutics, and the discovery of compounds that directly stimulate GLUT1 function. This work investigates how small molecules stimulate and/or inhibit GLUT1-mediated glucose transport, either directly or through the AMPK pathway. Using sugar transport assays and docking analyses to explore Ligand–GLUT1 interactions and specificity of binding, we show that: 1) Ligands inhibit GLUT1 by competing with glucose for binding to the exofacial or endofacial sugar binding sites; 2) Subsaturating inhibitor concentrations stimulate sugar uptake; 3) Ligands inhibit GLUT1–, GLUT3– and GLUT4–mediated sugar uptake in HEK293 cells; and 4) Inclusion of a benzonitrile head group on endofacial GLUT1 inhibitors confers greater inhibitory potency. Furthermore, we investigated AMPK-regulated GLUT1 trafficking in cultured blood-brain barrier endothelial cells, and show that inhibition of GLUT1 internalization is not responsible for increased cell surface levels of GLUT1 observed with AMPK activation in these cells. This study provides a framework for screening candidate GLUT1 inhibitors for specificity, and for optimizing drug design and delivery. Our data on transport stimulation at low inhibitor concentrations support the idea that GLUT1 functions as a cooperative oligomer of allosteric alternating access subunits.

Symptom severity among veteran survivors with partial or full posttraumatic stress disorder (PTSD) continues to increase, with approximately 40% of U.S. veterans reporting significant symptomology 10 years after initial onset of the condition. Veteran survivors often struggle to find therapeutic interventions that meet their specific needs and have a difficult time maintaining a therapy that is both equitable and evidenced based. Grounded in the Rogerian, client-centered theory, the purpose of this qualitative collective exploratory case study was to explore the effects of equine-facilitated psychotherapy with 3 veteran survivors with partial or full PTSD. A 4-stage process was used to collect data, including initial semistructured interviews, observations, photo-interviews, and researcher interpretations of photo-interviews. Data were transcribed, analyzed, and coded into within-case themes and cross case-themes. The principle findings revealed that veteran survivors with partial or full PTSD engaged in an equine-facilitated psychotherapy program had both here-and-now experiences and relational connection experiences with the horse. Many other significant details provided insight into the veteran participants' experiences with equine-facilitated psychotherapy, such as trust, connection, nonverbal communication, awareness, peace, decompression, communication, empathic reflection, congruence, reciprocity, concern, respect, and selflessness. These findings provide social change implications that may inform mental health professionals and counselor educators about the benefits of equine-facilitated psychotherapy with veteran survivors with partial or full posttraumatic stress disorder; the findings also provide structure to the use of equine-facilitated psychotherapy as an adjunct and/or alternative to traditional posttraumatic stress disorder treatments.

The aim of this phenomenological qualitative study was to capture and understand the essence of the lived experiences of individuals after participating in equine facilitated psychotherapy (EFP). In that the experiences of participants after exposure to EFP have not previously been examined, this study adds to the literature on this innovative therapy. Theoretical viewpoints on animal assisted therapy and solution-focused brief therapy (SFBT) were explored, as well as how the SFBT methodology compares to techniques used in EFP. Using interviews, the study involved capturing participants' experiences by collecting their descriptions of their involvement with EFP, identifying the specific experiences they noted, ascertaining what the participants did with these experiences, and discerning themes or patterns in the interview data. A purposive sample of 10 adults who had participated in EFP participated in interviews, the data from which were analyzed by hand coding. Analysis showed improved quality of life with improvements in overall well-being and in participants' relationships. The findings of this research study may lead to additional research in this area and may promote the establishment of consistent techniques in EFP, proper credentialing of those who use EFP, and applicable regulatory standards. By exploring the lived experiences of individuals who have participated in EFP, providers may be able to delve more deeply into the curative factors that may be at work with this type of therapy.

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This dissertation was completed in partial fulfillment of the Doctor of Public Health program at East Tennessee State University. The dissertation follows the Manuscript Format which includes three manuscripts, these manuscripts regard familial sex trafficking and are as follows, a scoping review, an empirical study on regional familial sex trafficking, and a policy implications paper on criminal record relief for survivors of familial sex trafficking. The scoping review includes eight peer reviewed studies on familial sex trafficking and a summary of grey literature. Thematic analysis was used to map the existing literature onto the Three Ps of human trafficking: Prevention, Protection, Prosecution, and a 4th crosscutting element Partnership. The scoping review summarized these characteristics and found that familial sex trafficking remains widely understudied and needs better data, awareness, training, and collaborations. The empirical study interviewed 10 individuals who had expertise or lived experience working with perpetrators or victims/survivors of familial sex trafficking in East Tennessee. This study found that like previous literature, the social determinants of health influence this form of abuse. Further, it contextualized the experience of traffickers, their vulnerabilities, and how these vulnerabilities tempted them into familial sex trafficking to meet their basic needs. This study highlights gaps in awareness, training, and programming for prevention and survivor support. The final paper, the policy implication paper, leverages data collected from the empirical study to demonstrate the reality of forced criminality during familial sex trafficking victimization and how criminal records impact recovery. This paper further explores the implications for developing policy and promising practices to further inform policy development. In summary, these manuscripts were carried out in partial fulfillment of the Doctor of Public Health program at East Tennessee State University.

This dissertation employs a case study research design and reviews literature from the field of animal assisted therapy (AAT) with an emphasis on equine facilitated psychotherapy (EFP), as a possible therapeutic alternative for treating inappropriate aggressive behaviour in a young boy. The research suggests that Horse Whispering can indeed be regarded as a viable therapeutic technique when intent on breaking the aggressive behaviour cycle. / Educational Studies / M.Ed.

Human Trafficking is the modern-day equivalent of slavery. Those who perpetuate it do so because it is lucrative to invest in a product that can be resold multiple times (Ellis, 2017). The recent advancement of technology has tremendously helped traffickers thrive in the illicit business of Human Trafficking. Social media websites, online classifieds, and mobile applications are quickly becoming some of the major mediums perpetrators utilize to traffick their victims (Latonero, 2012). The purpose of this study was to examine social service workers’ knowledge on the use of technology for Human Trafficking. Face-to-face interviews were conducted and the participants were asked a series of open-ended questions. Participants in this study were recruited from a social service agency in San Bernardino County that frequently serves survivors of Human Trafficking. The study found that all of the participants felt confident in their ability to serve survivors of Human Trafficking. The study also found that most participants had little to no training in regards to the topic, and did not have any knowledge of the kind of language used by perpetrators for Human Trafficking. In order to address the different components of the issue, social service workers should advocate for trainings in order to stay competent in regards to the issue. Social service agencies who serve the community must offer appropriate interventions and services for Human Trafficking survivors. Finally, social service workers should continue to advocate for legislature and policy that will help survivors recover versus legislation and policy that is punitive.

This dissertation employs a case study research design and reviews literature from the field of animal assisted therapy (AAT) with an emphasis on equine facilitated psychotherapy (EFP), as a possible therapeutic alternative for treating inappropriate aggressive behaviour in a young boy. The research suggests that Horse Whispering can indeed be regarded as a viable therapeutic technique when intent on breaking the aggressive behaviour cycle. / Educational Studies / M.Ed.