Submitted by Automa??o e Estat?stica (sst@bczm.ufrn.br) on 2017-07-03T12:09:01Z No. of bitstreams: 1 LaraMendesBragaRigoti_DISSERT.pdf: 4962466 bytes, checksum: d2c92cb0817a9cca89c864c0d34e8303 (MD5) / Approved for entry into archive by Arlan Eloi Leite Silva (eloihistoriador@yahoo.com.br) on 2017-07-05T12:32:30Z (GMT) No. of bitstreams: 1 LaraMendesBragaRigoti_DISSERT.pdf: 4962466 bytes, checksum: d2c92cb0817a9cca89c864c0d34e8303 (MD5) / Made available in DSpace on 2017-07-05T12:32:30Z (GMT). No. of bitstreams: 1 LaraMendesBragaRigoti_DISSERT.pdf: 4962466 bytes, checksum: d2c92cb0817a9cca89c864c0d34e8303 (MD5) Previous issue date: 2017-03-14 / O acolhimento institucional de crian?as e adolescentes ? um tema complexo para discuss?o e pesquisa dentro e fora do campo psicol?gico. A presente pesquisa buscou compreender como dois adolescentes em situa??o de acolhimento institucional, d?o sentido ?s suas hist?rias de vida antes do acolhimento, durante a institucionaliza??o, e como pensam que ser? depois que sa?rem do abrigo. Foi utilizado o scrapbook como instrumento l?dico, de acesso ?s narrativas, tendo como suporte te?rico a Sociologia cl?nica, e como perspectiva metodol?gica a Pesquisa (Auto) biogr?fica. Esse estudo mostrou que o acolhimento institucional se d? por diferentes viola??es de direitos as crian?as e adolescentes, reflexo de um quadro de vulnerabilidade social. A medida protetiva de abrigo, que visa garantir o direito ? conviv?ncia familiar e comunit?ria, n?o funciona bem assim na pr?tica. O abrigo ? um espa?o complexo e controverso, que muitas vezes n?o cumpre o seu papel. Ele pode ser um lugar de reprodu??es de vulnerabilidades e desprote??o. E pode ser tamb?m um lugar de prote??o, de rela??es afetivas, refer?ncias e sa?das existenciais. Ao dar voz a esses sujeitos, o trabalho oportunizou um espa?o de escuta e de reflex?o sobre suas pr?prias hist?rias de vida e perspectivas p?s-institucionaliza??o, possibilitando ressignifica??es. Talvez o acolhimento institucional seja um ?mal necess?rio? ou seja um ?bem indesejado?. O mais importante ? que, dentro do abrigo ou n?o, eles querem ser ouvidos, ser amados, ser acolhidos. / Childhood protection is a complex topic of discussion and researches inside and outside psychological Field. The present research sought to understand how two adolescents in a situation of foster institution, give meaning to their personal histories before the received and during the institutionalization, and how they think it will be after they leave the shelter. Scrapbook was used as a ludic instrument, access to the narratives, having as theoretical support Clinical Sociology, and as a methodological perspective the (Auto)biography research. This study showed that foster institution is due to different rights violations of children and adolescents, reflecting a framework of social vulnerability. The protective measure of shelter, aimed at guaranteeing the right to family and community coexistence, does not work well in practice. The shelter is a complex and controversial place, which often does not fulfill its role. It can be a place of reproductions of vulnerabilities and unprotection. And it can also be a place of protection, affective relations, references and existential exits. By giving voice to these subjects, the work provided a space for listening and reflection on their own life histories and post-institutionalization perspectives, allowing re-significances. Foster institution may be a "necessary evil" or an "unwanted good". Most important is, within the shelter or not, they want to be heard, to be loved, to be sheltered.

Submitted by Automa??o e Estat?stica (sst@bczm.ufrn.br) on 2017-08-01T13:15:55Z No. of bitstreams: 1 ThalisPaulinoGinani_DISSERT.pdf: 2052482 bytes, checksum: f2a1d3aca81cb2c9e57898488db570eb (MD5) / Approved for entry into archive by Arlan Eloi Leite Silva (eloihistoriador@yahoo.com.br) on 2017-08-03T23:31:00Z (GMT) No. of bitstreams: 1 ThalisPaulinoGinani_DISSERT.pdf: 2052482 bytes, checksum: f2a1d3aca81cb2c9e57898488db570eb (MD5) / Made available in DSpace on 2017-08-03T23:31:00Z (GMT). No. of bitstreams: 1 ThalisPaulinoGinani_DISSERT.pdf: 2052482 bytes, checksum: f2a1d3aca81cb2c9e57898488db570eb (MD5) Previous issue date: 2017-05-24 / Coordena??o de Aperfei?oamento de Pessoal de N?vel Superior (CAPES) / O Brasil det?m uma das maiores reserva de ?gua doce do mundo, todavia h? regi?es com escassez h?drica como ? o caso da regi?o semi?rida do Nordeste brasileiro. Nesse sentido, com o intuito de contribuir para uma gest?o adequada dos recursos h?dricos ? imprescind?vel ? realiza??o de um diagn?stico da regi?o para an?lise da adequa??o entre disponibilidade e demanda h?dricas. O objetivo deste trabalho ? desenvolver um modelo da confiabilidade dos sistemas de abastecimento de ?gua no atendimento das demandas humanas na Bacia do rio Serid? e classificar os munic?pios de acordo com o balan?o h?drico entre disponibilidade e demanda. Para o desenvolvimento do modelo utilizou-se duas metodologias, a regress?o linear m?ltipla via Stepwise e a regress?o por componentes principais para a escolha das vari?veis do segundo modelo. Conclui-se que o modelo desenvolvido pela primeira metodologia foi o mais representativo para a Bacia do Rio Serid?. Com o balan?o h?drico entre demanda e disponibilidade verificou-se que mais de 50% dos munic?pios apresentam esta vari?vel acima do valor unit?rio. Desta forma fica ent?o evidente a necessidade do levantamento de informa??es para o planejamento de a??es que mudem esta realidade, tendo como base o ranqueamento das cidades em fun??o de vari?vel (is) que as caracterize(m) os munic?pios em fun??o da qualidade dos sistemas de abastecimento de ?gua no atendimento das suas demandas urbanas / Brazil has one of the largest reserves of fresh water in the world, but there are regions with water scarcity, as is the case in the semi-arid region of the Brazilian Northeast. In this sense, in order to contribute to an adequate management of water resources, it is essential to carry out a diagnosis of the region to analyze the adequacy between water availability and demand. The objective of this work is to develop a model of the reliability of water supply systems to meet human demands in the Serid? River Basin and to classify municipalities according to the water balance between water availability and demand. For the development of the model two methodologies were used, the multiple linear regression via Stepwise and the regression by principal components for the choice of the variables of the second model. We conclude that the model developed by the first methodology was the most representative for the Serid? River Basin. With the water balance between demand and availability, it was verified that more than 50% of the municipalities present this variable above the unit value. In this way, it becomes evident the need to collect information to plan actions that change this reality, based on the ranking of cities as a function of variables (s) that characterize municipalities in function of the quality of the systems of Water supply to meet their urban demands

Submitted by Jorge Silva (jorgelmsilva@ufrrj.br) on 2017-06-20T20:50:53Z No. of bitstreams: 1 2015 - Wagner Scheidt.pdf: 2317119 bytes, checksum: 23f086e6fa2757e68ba3e14cc8297249 (MD5) / Made available in DSpace on 2017-06-20T20:50:53Z (GMT). No. of bitstreams: 1 2015 - Wagner Scheidt.pdf: 2317119 bytes, checksum: 23f086e6fa2757e68ba3e14cc8297249 (MD5) Previous issue date: 2015-10-27 / CAPES / The bacteria Herbaspirillum seropedicae is established as diazotrophic, nitrogen fixing, gram-negative, vibrioid, ?-proteobacteria, showing ability to synthesize phytohormones growth promoters in plants. The goal of this work was to improve the fermentation production of H. seropedicae, seeking the maximum production of cells and indole compounds with process cost reduction. The optimization of the culture medium Yeast Dextrose Glucose Sucrose - DYGS ? was conducted through fermentation tests on shaker. The original carbon sources were replaced, and the concentrations of nitrogen and nutrient sources were optimized through the Central Composite Rotational Design - CCRD (2?). The simplified culture medium obtained was composed of glycerol (5.5g L-1), yeast extract (2.8 g L-1), MgSO4?7H2O (0.5 g L-1) e K2HPO4 (0.5 g L-1). This medium provided an increase of 87.5 % in biomass production, and is 49.8 % cheaper than the traditional culture medium DYGS. An experimental design CCRD (2?) was carried with the purpose of improving the operational conditions of the bioreactor and the best growing condition was obtained at 35 ?C, 3 LPM of aeration rate, and 180 RPM of agitation speed. Under these conditions, and using the previously optimized culture medium, the maximum cell production (X= 3.23 g L-1) as well as the maximum metabolic product (P= 9.02mg L-1) were obtained. It was also tested a culture medium with glycerol and yeast extract concentrations increased to 8.0 g L-1 and 4.0 g L-1, respectively. Under these conditions, it was observed that the maximum cell production and metabolic product increased to X = 4.18 g L-1 and P = 11.74 mg L-1, respectively. An appropriate mathematical computer simulation model was developed and used to describe the dynamic behavior of the main variables of fermentation bioreactor. However, there was some difficulty to reproduce appropriately the profiles of all variables, probably due to the specific characteristics of this fermentation process, and the necessity of a mathematical model that takes into account parameters associated to limitations and inhibitions caused by substrate and product. / A bact?ria Herbaspirillum seropedicae ? estabelecida como sendo diazotr?fica fixadora de nitrog?nio, gram-negativa, vibri?ide, membro da classe ? das proteobact?rias, apresentando a capacidade de produ??o de fitohorm?nios promotores do crescimento em plantas. Este trabalho apresentou como objetivos aprimorar o processo fermentativo de produ??o de H. seropedicae, buscando a m?xima produ??o de c?lulas e compostos ind?licos, com redu??o de custos do processo. Realizou-se a otimiza??o do meio de cultura Dextrose Yeast Glucose Sucrose modificado ? DYGS ? atrav?s de ensaios fermentativos em shaker. Substitu?ram-se as fontes de carbono originais e aperfei?oaram-se as concentra??es dos demais componentes atrav?s da matriz experimental 2? obtida atrav?s do Delineamento Composto Central Rotacional ? DCCR. Obteve-se um meio de cultura simplificado, composto por glicerol (5,5 g L-1), extrato de levedura (2,8 g L-1), MgSO4?7H2O (0,5 g L-1) e K2HPO4 (0,5 g L-1) que se apresentou 49,8 % mais barato e com capacidade de produ??o de c?lulas 87,5 % maior quando comparado ao meio DYGS. Atrav?s da DCCR (2?) aprimorou-se tamb?m as condi??es operacionais de biorreator, obtendo-se a melhor condi??o de cultivo a temperatura de 35 ?C, aera??o de 3 LPM e agita??o de 180 RPM. Nestas condi??es, com o meio de cultura otimizado, obteve-se a produ??o celular m?xima de X = 3,23 g L-1 e de produto metab?lico m?ximo de P = 9,02 mg L-1. Testou-se um meio de cultura com concentra??es de glicerol e de extrato de levedura aumentadas para 8,0 g L-1 e 4,0 g L-1, respectivamente, sendo observada uma produ??o celular m?xima de X = 4,18 g L-1 e de produto metab?lico m?ximo de P = 11,74 mg L-1. Avaliou-se atrav?s de simula??o computacional, o modelo matem?tico mais adequado para descrever o comportamento din?mico das principais vari?veis das fermenta??es em biorreator. Contudo, observou-se uma alta dificuldade em descrever adequadamente os perfis de todas as vari?veis avaliadas, provavelmente devido ?s caracter?sticas espec?ficas deste processo fermentativo e ? necessidade de um modelo que leve em considera??o par?metros de limita??es e inibi??es causadas pelo substrato e produto.

Submitted by SBI Biblioteca Digital (sbi.bibliotecadigital@puc-campinas.edu.br) on 2018-02-26T12:24:42Z No. of bitstreams: 1 ANDRESSA GOTIERRA.pdf: 1447432 bytes, checksum: 56b6da2071e006773f88cf31adb65aa1 (MD5) / Made available in DSpace on 2018-02-26T12:24:42Z (GMT). No. of bitstreams: 1 ANDRESSA GOTIERRA.pdf: 1447432 bytes, checksum: 56b6da2071e006773f88cf31adb65aa1 (MD5) Previous issue date: 2017-02-17 / The research investigated conceptions of Philosophy in courses of Pedagogy in Brazil, from Political Pedagogical Projects of fifteen Brazilian universities, three from each region of the country, one private, one federal and one state. The selection of the courses considered those who obtained the highest mark in the National Student Performance Examination (Enade), during the years of 2005, 2008 and 2011. Tacitly, the evaluation of Enade was considered relevant and rigorous. The implicit quality of those courses. If the Philosophy is important and the educational legislation bases this assertion, from the Law of Directives and Bases n? 9394/96 or the Resolutions of the National Council of Education / CP, of number 1 and 2, of July of 2015, respectively, the problem Of the research consisted of the question: what conceptions of Philosophy of the courses of Pedagogy of the Brazilian universities attributes as the basis of their Pedagogical Projects of Course? The objective of the research consisted in examining the Projects of the fifteen selected institutions, in order to clarify the explicit or implicit philosophical conceptions present in them. For this, there was a documentary search in the portals of the universities and the pedagogical proposals were analyzed. The selected material was read and categorized in the following terms: (i) Philosophy and critical formation; (Ii) Teacher dialectic formation; (Iii) Philosophy and training for autonomy. The categories were constructed in the light of classics of pedagogical thought, Marx and Dewey, epistemological bases common to the courses studied. The results pointed out that the courses assume critical philosophical conceptions in their curricular matrices, in the sense of consolidating a formation for the social transformation. The presence of Philosophy in the courses is not limited to the legal dimension, but, it configures a conception of formation that points to the profile of the student who wishes to construct himself, that is, a rigorous teacher in his formation, who has the capacity to understand the forces Of teacher education and educational policies. From the point of view of the curricular structure of the courses, all contemplate Philosophy as a discipline, be it the general or the Philosophy of Education. The contents of curricula presented classic philosophical thought, but many commentators. Therefore, it can be deduced from the documentary analyzes that, above all, the disciplines related to philosophy, in the attempt to present different philosophical perspectives, used theoretical foundations through didactic manuals, making superficial the perspectives that they intended to present. In addition to these manuals, it was also noticed the presence of authors who defend the critical historical perspective as support of the courses, although the projects pointed to a plural perspective of formation. Therefore, it was evident that the philosophical conceptions originating from the critical historical perspective have a great influence on the pedagogical courses analyzed, however, the need to review the Pedagogical Projects is necessary, since to the extent that they aim at a critical and conscious formation it is necessary Before other theoretical knowledge is provided that allow the teacher autonomy in making decisions about his or her own practice. / A pesquisa investigou concep??es de Filosofia em cursos de Pedagogia no Brasil, a partir de Projetos Pol?ticos Pedag?gicos de quinze universidades brasileiras, tr?s de cada regi?o do pa?s, uma particular, uma federal e uma estadual. A sele??o dos cursos considerou aqueles que obtiveram maior nota no Exame Nacional de Desempenho de Estudantes (Enade), durante os anos de 2005, 2008 e 2011. De forma t?cita, considerou-se relevante e rigorosa a avalia??o do Enade, por isto, sup?s-se a qualidade impl?cita dos referidos cursos. Se a Filosofia ? importante e a legisla??o educacional fundamenta esta asser??o, desde a Lei de Diretrizes e Bases n? 9394/96 ou as Resolu??es do Conselho Nacional de Educa??o/CP, de n?mero 1 e 2, de julho de 2015, respectivamente, o problema da pesquisa consistiu na pergunta: que concep??es de Filosofia os cursos de Pedagogia das universidades brasileiras assumem como base de seus Projetos Pedag?gicos de Curso? O objetivo da pesquisa consistiu no exame dos Projetos das quinze institui??es selecionadas, no sentido de esclarecer as concep??es filos?ficas expl?citas ou t?citas presentes neles. Para isso, houve busca documental nos portais das universidades e procedeu-se ? an?lise das propostas pedag?gicas. O material selecionado foi lido e categorizado nos seguintes termos: (i) Filosofia e forma??o cr?tica; (ii) Forma??o dial?tica do professor; (iii) Filosofia e forma??o para a autonomia. As categorias foram constru?das ? luz de cl?ssicos do pensamento pedag?gico, Marx e Dewey, bases epistemol?gicas comuns aos cursos estudados. Os resultados apontaram que os cursos assumem concep??es cr?ticas filos?ficas em suas matrizes curriculares, no sentido de consolidar uma forma??o para a transforma??o social. A presen?a da Filosofia nos cursos n?o se reduz ? dimens?o legal, mas, configura uma concep??o de forma??o que aponta para o perfil do estudante que deseja construir-se, isto ?, um professor rigoroso em sua forma??o, que tenha capacidade de compreender as for?as dial?ticas da forma??o docente e das pol?ticas educacionais. Do ponto de vista da estrutura curricular dos cursos, todos contemplam a Filosofia como disciplina, seja a geral ou a Filosofia da Educa??o. Os conte?dos dos planos curriculares apresentaram cl?ssicos do pensamento filos?fico, mas, muitos comentadores. Portanto, pode-se depreender das an?lises documentais que, sobretudo, as disciplinas relacionadas ? filosofia, na tentativa de apresentar diferentes perspectivas filos?ficas usaram fundamenta??es te?ricas por meio de manuais did?ticos, tornando superficiais as perspectivas que objetivaram apresentar. Al?m desses manuais, foi percebida tamb?m a presen?a de autores que defendem a perspectiva hist?rico cr?tica como sustenta??o dos cursos, embora os projetos apontassem para perspectiva plural de forma??o. Evidenciou-se, portanto, que as concep??es filos?ficas oriundas da perspectiva hist?rico cr?tica t?m muita influ?ncia sobre os cursos de Pedagogia analisados, entretanto, a necessidade em rever os Projetos Pedag?gicos se faz necess?ria, pois na medida que objetivam uma forma??o cr?tica e consciente ? necess?rio antes que se proporcione conhecimentos te?ricos outros que possibilitem ao professor autonomia na tomada de decis?o sobre sua pr?pria pr?tica.

Submitted by Setor de Tratamento da Informa??o - BC/PUCRS (tede2@pucrs.br) on 2016-08-24T17:00:02Z No. of bitstreams: 1 DIS_KARINA_SCHUH_REIF_COMPLETO.pdf: 744660 bytes, checksum: f597efbef90b167f817a2bab5d6f8647 (MD5) / Made available in DSpace on 2016-08-24T17:00:02Z (GMT). No. of bitstreams: 1 DIS_KARINA_SCHUH_REIF_COMPLETO.pdf: 744660 bytes, checksum: f597efbef90b167f817a2bab5d6f8647 (MD5) Previous issue date: 2016-03-23 / Coordena??o de Aperfei?oamento de Pessoal de N?vel Superior - CAPES / This research analyzes the experiences of women freedom after arrested period. Through the methodological approach of biographical narrative was possible to identify the main topics presented on the speeches that permeate freedom life after detention period. Biographical interviews were realized with seven women who were kept in Porto Alegre, Rio Grande do Sul, jail, being the interview of Marilene analyzed using sequential manner and the others evaluated in a global manner, following the pattern of biographical narratives analysis developed by Gabriele Rosenthal. The biographical narratives pointed three aspects: ex prisoner stigma, expressed as a latent manner such as the ways it appear in the biographical reports of Marilene and others interviewed. The second point presented is the reinforcement of gender roles, used to mitigate the image of ex-prisoners. The third point emphasized on the interviews and analyzed on the present work is the correction character that ex-prisoners accredited for the prison system. This research intends to contribute for freedom experiences comprehension after in jail period. Biographical narratives demonstrate that analysis of prior biographical periods, such incarceration and the prior experiences, might contribute to comprehend the way freedom is experienced. Among the outcomes of this work is the finding which, at least, the sample researched do not confirms the logical worked by the writers that the jail would work as a ?crime school?. The considerations leads to understanding of prisoners interviewed want a new opportunity. The priority given to family is used as motivation for not relapse. The research suggests the necessity to amplify, in future works, the number of interviews to be sequentially analyzed, with the purpose to build defined biographical typology for the experience of freedom after in jail period. / A presente pesquisa analisa viv?ncias de liberdade de mulheres no per?odo posterior ? pris?o. Por meio da abordagem metodol?gica de narrativas biogr?ficas foi poss?vel identificar os principais temas presentes nos discursos que permeiam a vida em liberdade ap?s per?odo de encarceramento. Foram realizadas entrevistas biogr?ficas com sete mulheres com passagem por uma penitenci?ria em Porto Alegre, Rio Grande do Sul, sendo que a entrevista de Marilene ? analisada de maneira sequencial e as demais avaliadas de forma global, seguindo o modelo de an?lise de narrativas biogr?ficas desenvolvido por Gabriele Rosenthal. As narrativas biogr?ficas evidenciaram principalmente tr?s aspectos. O estigma de ex-presas, expresso tanto de maneira latente quanto de forma manifesta nos relatos biogr?ficos de Marilene e das demais entrevistadas, ? um deles. O segundo aspecto ? o refor?o de pap?is de g?nero, utilizado como uma das formas para atenuar a imagem de egressas do pres?dio. O terceiro aspecto enfatizado nas entrevistas e analisado neste trabalho ? o car?ter corretivo que as egressas atribuem ao sistema prisional. Com esta pesquisa, pretende-se contribuir para a compreens?o das experi?ncias de liberdade ap?s um per?odo de encarceramento. Narrativas biogr?ficas apontam que a an?lise de per?odos biogr?ficos anteriores, como o pr?prio encarceramento e as experi?ncias anteriores a ele, podem contribuir para a compreens?o da maneira como a liberdade ? vivenciada. Entre os resultados do trabalho est? a constata??o de que, pelo menos, a amostra pesquisada n?o confirma a l?gica trabalhada por parte dos autores de que a cadeia funcionaria como uma ?escola do crime?. As considera??es levam ao entendimento de que as presas entrevistadas querem uma nova oportunidade. A prioridade dada ? fam?lia ? utilizada como motiva??o para n?o reincid?ncia. A pesquisa sugere a necessidade de ampliar, em trabalhos futuros, o n?mero de entrevistas a serem analisadas sequencialmente, com o objetivo de se construir tipologias biograficamente definidas para a viv?ncia da liberdade ap?s o encarceramento.

Submitted by Jorge Silva (jorgelmsilva@ufrrj.br) on 2017-04-18T20:10:31Z No. of bitstreams: 1 2010 - Cec?lia de Souza Ant?nio.pdf: 1613209 bytes, checksum: e560fdd88ef8707b79c6d4b0120730ec (MD5) / Made available in DSpace on 2017-04-18T20:10:31Z (GMT). No. of bitstreams: 1 2010 - Cec?lia de Souza Ant?nio.pdf: 1613209 bytes, checksum: e560fdd88ef8707b79c6d4b0120730ec (MD5) Previous issue date: 2010-04-20 / CAPES / The sugar cane is one of the major agricultural products in Brazil. The crop is able to associate with diazotrophic bacteria (fix nitrogen from the air), that may be located inside the plant tissue (entophytic). The diazotrophic bacteria are capable of promoting growth of sugar cane by means of biological nitrogen fixation (BNF) or production of hormones. But little is known about populations of these bacteria present in sugar cane. This work aimed to study diversity of the population and identify the isolates by molecular and physiological methods, as well as to assess the effectiveness of some isolates to promote plant growth of sugar cane in the field. In solid potato media, there were observed the formation of seven groups, with 95% of similarity, showing the great colonies morphology variation. Many isolates showed similar characteristics to the genus Gluconacetobacter, when analyzed in semi-solid LGI-P media and solid Potato-P and LGI-P media. Two isolates were most efficient in the endolar synthesis with production over 49 ?g/mL. All isolates were classified as Gram negative. Of the 36 isolates, 27.5% were similar to the standard strain RB 11366 (Burkholderia tropica), 45% to BR 11281 strain (Gluconacetobacter diazotrophicus) and 5% to the other patterns BR 11335 (Herbaspirillum seropedicae), BR 11504 (Herbaspirillum rubrisubalbicans) and BR 11145 (Azospirillum amazonense). Through the comparison of the sequencing of 16S rDNA with the NCBI GenBank isolate 215 was identified as belonging to species Gluconacetobacter diazotrophicus, the 179-1a belonging to Burkholderia tropica and the isolated 151-B, 211-A, and 219 to the gender Burkholderia. The inoculated strains 160-1 and 215 promoted an increase in the straw dry biomass (up to 0.7 Mg ha-1) and total nitrogen of flag leaf (above 69,7 kg ha-1), respectively in the tested varieties RB 72454 and RB 918,639. Only the 160-1 isolate was able to promote increase in biomass in the RB 867515 variety. Stalk yield was higher for the variety RB 918639 with 191.96 Mg ha-1. / A cana-de-a??car ? um dos principais produtos agr?colas do Brasil. A cultura ? capaz de se associar as bact?rias diazotr?ficas (fixam nitrog?nio do ar), que podem estar no interior do tecido da planta (endof?ticas). As bact?rias diazotr?ficas endof?ticas s?o capazes de promover o crescimento da cana-de-a??car por meio da fixa??o biol?gica do nitrog?nio (FBN) ou pela produ??o de fitorm?nios. Mas pouco se conhece sobre as popula??es presentes destas bact?rias em cana-de-a??car. O presente trabalho visou estudar a diversidade da popula??o e identificar estes isolados, atrav?s de m?todos moleculares e fisiol?gicos, assim como avaliar a efici?ncia de alguns isolados na promo??o de crescimento vegetal de plantas de cana-de-a??car no campo. Foi observada em meio s?lido Batata, com 95% de similaridade, a forma??o de sete grupos mostrando a grande varia??o morfol?gica de col?nias neste meio testado. Muitos isolados apresentaram caracter?sticas similares ao g?nero Gluconacetobacter, quando analisados em meio semi-s?lido LGI-P e s?lido Batata-P e LGI-P. Dois isolados foram mais eficientes na s?ntese de ind?les com produ??es acima de 49 ?g/mL. Todos os isolados foram classificados como Gram negativos. Dos 36 isolados avaliados, 27,5% foram semelhantes ? estirpe padr?o RB 11366 (Burkholderia tropica); 45% a BR 11281 (Gluconacetobacter diazotrophicus) e 5% aos demais padr?es BR 11335 (Herbaspirillum seropedicae), BR 11504 (Herbaspirillum rubrisubalbicans) e BR 11145 (Azospirillum amazonense). Atrav?s da compara??o do seq?enciamento do gene 16S rDNA com o NCBI GenBank o isolado 215 foi identificado com pertencente a esp?cie de Gluconacetobacter diazotrophicus , o 179-1A com pertencente a esp?cie Burkholderia tropica e os isolados 151-B, 211-A e 219 ao g?nero Burkholderia. As estirpes 160-1 e 215 inoculadas promoveram aumento na produ??o de biomassa seca da palha (acima de 0,7 Mg.ha-1) e nitrog?nio total da folha bandeira (acima de 69,7 kg.ha-1), respectivamente nas variedades RB 72454 e RB 918639 testadas. Apenas o isolado 160-1 foi capaz de promover um aumento de biomassa seca na variedade RB 867515. A produ??o de colmos foi maior para a variedade RB918639 com 191,96 Mg.ha-1.

Submitted by Sandra Pereira (srpereira@ufrrj.br) on 2018-11-23T11:46:06Z No. of bitstreams: 1 2010 - Juliano Br?s Zanetti.pdf: 621874 bytes, checksum: 7668a4e8b8cab8a537ab2b436d55b596 (MD5) / Made available in DSpace on 2018-11-23T11:46:07Z (GMT). No. of bitstreams: 1 2010 - Juliano Br?s Zanetti.pdf: 621874 bytes, checksum: 7668a4e8b8cab8a537ab2b436d55b596 (MD5) Previous issue date: 2010-07-27 / Coordena??o de Aperfei?oamento de Pessoal de N?vel Superior, CAPES, Brasil. / The production of plant biomass for alternative renewable energy currently represents a great challenge to research. The release of CO2 from its use constitutes only the CO2 recycling that was removed from the atmosphere by photosynthesis, which in the long term, represents a valuable alternative for bio-energy. Elephant grass (Pennisetum purpureum Schum.) is, among grasses, that with possibly the highest capacity for dry matter production, with other favorable characteristics for energy production. The aim of this study was to identify elephant grass genotypes with high biomass production and nitrogen accumulation under the inoculation with a mixture of N2-fixing bacteria. Two studies were conducted in Serop?dica, Rio de Janeiro State. The first experiment was conducted in a concrete tank filled with a Ndeficient Acrisol. The experimental design was randomized blocks in split plots with four replications. The plots consisted of the inoculation treatments (inoculated and uninoculated), and sub-plots for the five elephant grass genotypes: Cameroon, BAG 02, Roxo, Clone CNPGL 93 41 1 and CNPGL 91 F06-3. The second experiment was carried out on a Planosol and the two genotypes, BAG 02 and Cameroon, with inoculation treatments, inoculation + 100 kg of N ha-1, 100 kg of N ha-1 and a zero N control. The experimental design was randomized blocks in a factorial model with four replications. In these studies the following results were obtained: 1. Dry matter production (DM) ranged from 16 to 41 Mg of MS ha-1 year-1 when the varieties were grown in the Acrisol. In the Planosol the dry biomass varied between 48 and 56 Mg MS ha-1 in two crops over a 11-month period of cultivation. 2. In relation to the total N accumulated in shoots, the values ranged between 64 and 158 kg ha-1 when the genotypes were grown in Acrisol and between 297 and 441 kg ha-1 when the genotypes were cultivated in the Planosol. For these parameters, N accumulation and biomass yield, the best genotypes were: CNPGL F 06-3 and Cameroon followed by CNPGL 93 41 1 and BAG 02 genotype. 3. The C/N ratio was directly influenced by soil type, and the ratio observed in the Acrisol was approximately 50% higher when compared to the Planosol. 4. The calorific value and fiber content, cellulose, lignin and ash, which are related to the quality of the material, in general were not affected by genotype, or by treatment. The elephant grass biomass had positive qualitative and quantitative characteristics to be used for renewable energy production and the Cameron CNPGL F 06-3 genotype, followed by BAG CNPGL 93 41 02 genotype were the most promising. The genotypes studied no showed response of biomass production or N accumulation to inoculation with diazotrophic bacteria. / A produ??o de material energ?tico alternativo renov?vel, tal como a biomassa vegetal, representa hoje um dos grandes desafios para a pesquisa. No caso da biomassa, a libera??o de CO2 pelo seu uso representa apenas a reciclagem do CO2 que foi retirado da atmosfera pela fotoss?ntese, indicando que, em longo prazo, esta ser? uma das alternativas energ?ticas mais seguras. O capim-elefante (Pennisetum purpureum Schum.) est? entre as gram?neas de maior capacidade de produ??o de mat?ria seca, com caracter?sticas qualitativas favor?veis para a produ??o de energia. O objetivo deste trabalho foi identificar gen?tipos de capim-elefante para alto rendimento de biomassa, ac?mulo de N sob a inocula??o das plantas com uma mistura de bact?rias diazotr?ficas. Foram realizados dois estudos de campo localizados no munic?pio de Serop?dica, RJ. O primeiro experimento realizado em um tanque de concreto preenchido com Argissolo pobre em N dispon?vel. O delineamento experimental foi o de Blocos ao Acaso em parcelas divididas, com quatro repeti??es. As parcelas constitu?das pelos tratamentos de inocula??o (inoculado e n?o inoculado), e as sub-parcelas compostas pelos cinco gen?tipos de capim-elefante: Cameroon, BAG 02, Roxo, Clone CNPGL 93 41 1 e CNPGL 91 F06-3. No segundo experimento, realizado no campo em um Planossolo, os dois gen?tipos, BAG 02 e Cameroon foram avaliados sob os tratamentos de inocula??o, inocula??o + 100 kg de N.ha-1, 100 kg de N.ha-1, e o controle. O delineamento experimental utilizado foi o de Blocos ao Acaso em esquema fatorial com quatro repeti??es. Nestes estudos encontraram-se os seguintes resultados: 1. A produ??o de mat?ria seca (MS) que variou entre 16 e 41 Mg de MS.ha-1.ano-1, quando os gen?tipos foram cultivados no Argissolo. No Planossolo, o rendimento de biomassa seca variou entre 48 e 56 Mg.MS.ha-1 sob duas colheitas realizadas, em 11 meses de cultivo. 2. Em rela??o ao total de N acumulado na parte a?rea, os valores variaram entre 64 e 158 kg.ha-1 quando os gen?tipos foram cultivados no Argissolo, e entre 297 e 441 kg.ha-1 quando os gen?tipos foram cultivados no Planossolo. Para os par?metros, ac?mulo de N e rendimento de biomassa, os gen?tipos que mais se destacaram foram: CNPGL F 06-3 e Cameroon seguido dos gen?tipos BAG 02 e CNPGL 93 41 1. 3. A rela??o C/N foi influenciada diretamente pelo tipo de solo, sendo que no Argissolo as rela??es encontradas foram em torno de 50% maiores, quando comparadas com o Planossolo. 4. O poder calor?fico e os teores de fibra, celulose, lignina e cinzas, que est?o relacionados ? parte qualitativa do material, em geral, n?o foram influenciados pelos gen?tipos, nem pelos tratamentos avaliados. A biomassa do capim-elefante apresentou caracter?sticas qualitativas e quantitativas positivas para ser utilizado na produ??o de energia renov?vel, sendo os gen?tipos CNPGL F 06-3 e Cameron, seguido dos gen?tipos BAG 02 e CNPGL 93 41 1 os mais indicados. Os gen?tipos em estudo n?o apresentaram resposta para rendimento e acumula??o de N pela biomassa ? inocula??o com bact?rias diazotr?ficas.

Paraffin is used as a main household energy source for cooking, lighting and heating by low-income communities in South Africa. It is highly inflammable and spillages from paraffin can be considered as one of the major causes of fires that lead to the destruction of dwellings in the informal settlement. The situation is made worse due to the close proximity of the dwellings to each other which cause the fires to spread very quickly from one dwelling to the next leaving suffering and most often death in its wake (Schwebel et al., 2009:700). It has been shown by Muller et al. (2003:2018) that most of the informal rural communities use paraffin in non-ventilated and windowless environments and this causes major respiratory problems. The government has made a huge effort towards replacing paraffin as main cooking fuel in rural and informal settlements with ethanol gel. Ethanol gel is a healthier, safer alternative to paraffin because ethanol gel does not burn unless it is contained within a cooking device that concentrates the flame. It also fails to emit lung irritants or other dangerous chemical vapours when burned indoors (Bizzo et al., 2004:67). Commercial ethanol gels are manufactured with imported gelling agents that make their costs unaffordable to the rural poor communities. It is the objective of this study to determine whether gelling agents extracted from the local endemic species of cactacea viz. Opuntia fiscus-indica and Cereus Jamacaru can be used to synthesise ethanol gel comparable or better than the commercial gels. The two species chosen have been declared pests (Nel et al., 2004:61) and are continuously uprooted from arable land and burned by local farmers (Van Wilgen et al., 2001:162) This study showed that Opuntia ficas-indica stems gave a better cellulose yield (15.0 ± 6.7 wt. %) than Cereus Jamacaru (11.5 ± 7.8wt %). Chemical composition analyses and FT-IR analyses showed that the hemicelluloses and lignin were completely removed from the extracted cellulose and the extraction was more effective for Opuntia ficasindica than for Cereus Jamacaru. Ethanol gel produced by using the extracted cellulose, as was investigated during this study, was compared to commercial gels with respect to viscosity, burn time, calorific values and residue and a good comparison was obtained. / Thesis (M.Sc. Engineering Sciences (Chemical Engineering))--North-West University, Potchefstroom Campus, 2012.

Paraffin is used as a main household energy source for cooking, lighting and heating by low-income communities in South Africa. It is highly inflammable and spillages from paraffin can be considered as one of the major causes of fires that lead to the destruction of dwellings in the informal settlement. The situation is made worse due to the close proximity of the dwellings to each other which cause the fires to spread very quickly from one dwelling to the next leaving suffering and most often death in its wake (Schwebel et al., 2009:700). It has been shown by Muller et al. (2003:2018) that most of the informal rural communities use paraffin in non-ventilated and windowless environments and this causes major respiratory problems. The government has made a huge effort towards replacing paraffin as main cooking fuel in rural and informal settlements with ethanol gel. Ethanol gel is a healthier, safer alternative to paraffin because ethanol gel does not burn unless it is contained within a cooking device that concentrates the flame. It also fails to emit lung irritants or other dangerous chemical vapours when burned indoors (Bizzo et al., 2004:67). Commercial ethanol gels are manufactured with imported gelling agents that make their costs unaffordable to the rural poor communities. It is the objective of this study to determine whether gelling agents extracted from the local endemic species of cactacea viz. Opuntia fiscus-indica and Cereus Jamacaru can be used to synthesise ethanol gel comparable or better than the commercial gels. The two species chosen have been declared pests (Nel et al., 2004:61) and are continuously uprooted from arable land and burned by local farmers (Van Wilgen et al., 2001:162) This study showed that Opuntia ficas-indica stems gave a better cellulose yield (15.0 ± 6.7 wt. %) than Cereus Jamacaru (11.5 ± 7.8wt %). Chemical composition analyses and FT-IR analyses showed that the hemicelluloses and lignin were completely removed from the extracted cellulose and the extraction was more effective for Opuntia ficasindica than for Cereus Jamacaru. Ethanol gel produced by using the extracted cellulose, as was investigated during this study, was compared to commercial gels with respect to viscosity, burn time, calorific values and residue and a good comparison was obtained. / Thesis (M.Sc. Engineering Sciences (Chemical Engineering))--North-West University, Potchefstroom Campus, 2012.

Made available in DSpace on 2014-12-17T14:10:27Z (GMT). No. of bitstreams: 1 DiegoBSL_DISSERT.pdf: 829804 bytes, checksum: e8513b1969770df1630ff406301f2cb9 (MD5) Previous issue date: 2012-04-09 / This study assessed the level of knowledge, attitude and practice of Pap smear and human papillomavirus (HPV), in addition to analyzing the prevalence of genital HPV infection, Herpes Simplex Type 2 (HSV-2) and Chlamydia trachomatis in teenagers. The study consisted of two approaches, one based only on interviews conducted with adolescents enrolled in public schools or in public health facilities in the city of Natal. The other approach involved only a group of 132 adolescents enrolled among those admitted to two health units in Natal-RN. This second group of participants two specimens were collected for laboratory analysis: one was directed to prepare the blade for the Pap test, and other processed for DNA extraction for molecular analysis, focusing on the detection of HPV, HSV-2 and C . trachomatis. The presence of DNA of the three pathogens was investigated by the technique of polymerase chain reaction (PCR). The presence of each of the three pathogens was analyzed in terms of socio-demographic characteristics, as well as sexual and reproductive activity to identify risk factors for infection and development of lesions of the uterine cervix. The results show that the adolescents in this study had levels of knowledge and attitude very low, both in relation to cytology to HPV as though they have made a reasonable percentage of adequate practice exam and prevention of HPV infection. The overall prevalence of HPV infection was 54.5% and 48.2% in adolescents with normal cytology and 86.4% in those with abnormal cytology. We observed a higher proportion of cases of infection in the age group of 18 to 21. The prevalence of HPV infection was slightly higher among pregnant teenagers. The overall prevalence of HSV-2 infection was 13.6% and 11.8% in women with normal cytology and 22.7% in those with abnormal cytology. A higher proportion of cases of infection was found in the age group from 14 to 17, with a slightly higher prevalence among pregnant women. The C. trachomatis was found with an overall prevalence of 19.7% and 21.8% in adolescents with normal cytology and 9.1% in those with abnormal cytology. The prevailing rate was highest in the age group 18 to 21 years and in nonpregnant / O presente estudo avaliou o grau de conhecimento, atitude e pr?tica do exame de Papanicolaou e do v?rus do papiloma humano (HPV), al?m de analisar a preval?ncia da infec??o genital por HPV, Herpes Simples tipo 2 (HSV-2) e Chlamydia trachomatis em adolescentes. O estudo constou de duas abordagens, uma baseada apenas em entrevistadas realizadas com adolescentes recrutadas em escolas p?blicas ou em unidades de sa?de p?blica da cidade do Natal. A outra abordagem envolveu apenas um grupo de 132 adolescentes arroladas entre aquelas atendidas em duas unidades de sa?de de Natal-RN. Das participantes deste segundo grupo foram coletados dois esp?cimes para an?lise laboratorial: um foi destinado ? prepara??o de l?mina para o exame citol?gico de Papanicolaou, e outro processado para extra??o de DNA para an?lise molecular, visando ? detec??o de HPV, HSV-2 e da C. trachomatis. A presen?a do DNA dos tr?s pat?genos foi pesquisada por meio da t?cnica da rea??o em cadeia da polimerase (PCR). A presen?a de cada um dos tr?s pat?genos foi analisada em fun??o das caracter?sticas s?cio-demogr?ficas, bem com da atividade sexual e reprodutiva visando identificar fatores de risco para a infec??o e o desenvolvimento de les?es da c?rvice uterina. Os resultados mostram que as adolescentes deste estudo apresentaram graus de conhecimento e de atitude muito baixos, tanto em rela??o ao exame citol?gico quanto ao HPV, embora tenham apresentado um percentual razo?vel de pr?tica adequada do exame e da preven??o da infec??o pelo HPV. A preval?ncia global da infec??o pelo HPV foi de 54,5%, sendo 48,2% nas adolescentes com citologia normal e 86,4% naquelas com citologia alterada. Foi observada uma maior propor??o de casos da infec??o na faixa et?ria dos 18 aos 21. A preval?ncia da infec??o pelo HPV foi ligeiramente maior entre as adolescentes gr?vidas. A preval?ncia global da infec??o pelo HSV-2 foi de 13,6%, sendo 11,8% nas mulheres com citologia normal e 22,7% naquelas com citologia alterada. Uma maior propor??o de casos da infec??o foi encontrada na faixa et?ria dos 14 aos 17, sendo a preval?ncia ligeiramente maior entre as gr?vidas. A C. trachomatis foi encontrada com uma preval?ncia global de 19,7%, sendo 21,8% nas adolescentes com citologia normal e 9,1% naquelas com citologia alterada. A taxa de prevalecia foi maior na faixa et?ria dos 18 aos 21 anos e nas n?o gr?vidas