The aim of this diploma thesis is to draw up project documentation for new multifunctional building in the city of Vyškov. The discussed building is designed on flat terrain and has partial basement with four floors above ground. The basement contains technical facilities and storage area for building residents. The first floor contains commercial premises made of three stores: drugstore, flower shop and wine cellar. The second and third floor each contains two residential units. On the fourth floor is one spacious studio apartment.

京都大学 / 0048 / 新制・論文博士 / 博士(農学) / 乙第12807号 / 論農博第2792号 / 新制||農||1020(附属図書館) / 学位論文||H26||N4810(農学部図書室) / 80851 / (主査)教授 河田 照雄, 教授 金本 龍平, 教授 谷 史人 / 学位規則第4条第2項該当 / Doctor of Agricultural Science / Kyoto University / DFAM

京都大学 / 0048 / 新制・課程博士 / 博士(農学) / 甲第19051号 / 農博第2129号 / 新制||農||1033(附属図書館) / 学位論文||H27||N4933(農学部図書室) / 32002 / 京都大学大学院農学研究科農学専攻 / (主査)教授 白岩 立彦, 教授 奥本 裕, 准教授 中﨑 鉄也 / 学位規則第4条第1項該当 / Doctor of Agricultural Science / Kyoto University / DFAM

京都大学 / 0048 / 新制・論文博士 / 博士(農学) / 乙第13017号 / 論農博第2827号 / 新制||農||1042(附属図書館) / 学位論文||H28||N4964(農学部図書室) / 32945 / 京都大学大学院農学研究科農学専攻 / (主査)教授 土井 元章, 教授 裏出 令子, 教授 奥本 裕 / 学位規則第4条第2項該当 / Doctor of Agricultural Science / Kyoto University / DFAM

Astragalus ampullarioides and A. holmgreniorum are two rare endemics of southwestern Utah. Over two consecutive field seasons (2009-2010) we examined pre-emergent reproductive success, based on F/F and S/O ratios, from populations of both Astragalus ampullarioides and A. holmgreniorum, estimated the density of the soil seed bank of A. holmgreniorum as a measure of potential post-emergent reproductive success, and estimated seed persistence within the soil seed bank. Fruit/flower (F/F) ratios and seed/ovule (S/O) ratios varied significantly between populations and among years in both species, and showed low reproductive output in both taxa. In Astragalus ampullarioides F/F and S/O were 0.06±0.01 and 0.16±0.02, respectively (2009), and 0.14±0.01 and 0.41±0.02, respectively (2010). For Astragalus holmgreniorum F/F and S/O ratios were 0.11±0.01 and 0.38±0.02, respectively (2009), and 0.23±0.01 and 0.66±0.02, respectively (2010). Although Astragalus holmgreniorum exhibited a low soil seed bank density (4.3 seeds m-2), seed persistence data showed low a low percentage of seeds germinated during the first year in the soil seed bank. Seeds remaining in the seed bank maintained high percent viability. Soil seed persistence of Astragalus ampullarioides differed from A. holmgreniorum in that a high percentage of seeds germinated during the first year in the soil seed bank. A high percentage of viability in ungerminated seeds was also maintained in A. ampullarioides. Although these species differ in life histories and dependence on soil seed banks, an understanding of the strategies unique to each species will prove useful in management plans.

Currently the size and frequency of wildfires are increasing at a global scale, including arid ecosystems that exhibit great sensitivity to disturbance. Fire effects on plant pollination and reproductive success in deserts are largely unknown. Plant dependence on animal pollinators for reproduction can increase the risk of reproductive failure if pollination services are hindered or lost. Species that depend on few taxonomically related pollinator species are expected to be most negatively affected by disturbances that disrupt pollination interactions. To assess fire and isolation effects on reproductive success in desert plant communities, and how wildfire influences the pollination success of generalist and specialist pollinated plants, the number of flowers, fruits, and viable seeds produced by plants surviving in burned and unburned desert landscapes were compared. Fire increased flower production for wind and generalist pollinated plants, and did not affect specialist plant flower production. Increases may be associated with positive physiological responses exhibited by plants surviving in burned areas. Fire did not affect pollination services. Wildfire effects on fruit production were neutral or positive, and overall seed:ovule ratios varied by 3% or less in burned and unburned areas for each pollination strategy. Increasing isolation within burned areas did not affect fruit production for generalist or specialist pollinated plants, suggesting that pollination services are functional across expansive burned desert landscapes. Annual reproductive output varied between years in burned and unburned areas, and shifts likely resulted from variation in annual precipitation patterns. Reductions in landscape reproductive output may be partially compensated by increased per plant fruit and seed production and maintaining pollinator services across burned landscapes, providing native shrub communities the possibility to naturally recover from fire disturbances. Habitat disturbances can influence plant interactions with herbivores, in addition to pollinators. To understand how fire and ungulate herbivory affect reproductive success of specialist pollinated desert plants, reproductive effort, floral herbivory, and pollinator visitation and success, were compared for Yucca baccata, and Yucca brevifolia in burned and unburned areas of the Mojave Desert. Fire increased Y. baccata flowering from 12% to 22% of plants in burned areas, but had no effect on the number of flowers or fruits produced per plant. Fruit set and pollinator collection failed at all sampled Y. baccata individuals, while fire and herbivory had no effect on Y. brevifolia flower, fruit, and pollinator collection. Herbivores consumed 50% and 67% of floral stalks produced by Y. baccata in unburned and burned areas. Herbivores pose a clear threat to successful sexual reproduction for Y. baccata. Removal of ungulate herbivores during important flowering periods may still result in failed fruit and seed production if local pollinator reserves have been drastically reduced or lost.

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While pollinators and leaf herbivores have been a focus of research for decades, floral antagonists have been studied significantly less. Since floral antagonists can be as common as leaf herbivores and have strong impacts on plant reproduction, it is important to understand the role of floral antagonists in the ecology and evolution of flowers. I conducted four experiments to better understand the relationship between plants, floral traits, floral antagonists, and other plant-insect interactions. First, I manipulated resources (light and soil nutrients) that are known to have impacts on plants and floral traits to test how they affect floral antagonists and other plant-insect interactions. Plentiful resources increased the proportion of floral antagonists to visit flowers, but also increase tolerance of floral antagonists. Second, I manipulated flower bud gallers, a species-specific floral herbivore that destroys flowers, to test how it affected other plant-insect interactions, floral traits, and plant reproduction. Plants with flower bud gallers tended to have more pollinator visits, but this effect is due to a shared preference by gallers and pollinators for similar plants. Third, I manipulated florivory to examine how it affects subsequent plant-arthropod interactions, floral traits, and plant reproduction. Florivory had systemic effects on other plant-insect interactions, including leaf herbivores, and shifted the plant mating system towards more selfing. Additionally, I tested how several floral antagonists respond to floral attractive and defense traits to understand which floral traits are important in mediating antagonisms. Finally, I manipulated florivory, pollination, and nectar robbing to test for effects of multiple floral interactions on subsequent plant-insect interactions, floral traits, and plant reproduction. There were significant many-way interactions between the three treatments on subsequent plant-insect interactions and reproduction, indicating that the effect of one interaction depends on what other interactions are present. Understanding the role that floral antagonists play in plant ecology can help scientists determine which interactions are most important, and may help determine why some floral traits exist in their current state. Together, this work represents some of the most comprehensive research on the community consequences of floral antagonists, as well as the interplay between floral traits and floral interactions.

Insect pollinators serve a critical role in maintaining plant biodiversity and are especially susceptible to changes within their environment. To study the possible effects of seasonal variation in temperature, as well as climatic temperature increase on the plant-pollinator community, the relationship between bumblebee and flowering plant traits along an elevational gradient, representing warming-induced changes in plant community, were examined. Two hypotheses were tested; 1) if plant traits can predict visiting bumblebee proboscis length, and 2) if the relationship between plant traits and proboscis length is influenced by elevation, and the progression of the growing season. The study took place along an elevational gradient on Mt. Nuolja in Abisko National Park, Sweden. During surveys bumblebees were caught and measured. Flowers visited by captured bumblebees were collected, categorized by restrictiveness (i.e., whether or not the flower require a certain proboscis length, in order to access the nectar and pollen rewards) and floral traits measured (e.g., petal length). The results revealed that petal length was a significant predictor of bumblebee proboscis length, when taking restrictiveness into account. Furthermore, the relationship became weaker with increasing elevation for restrictive flowers but stronger for unrestrictive flowers. These findings show that trait-matching between bumblebees and flowers is an influential factor for flower selection and is affected by climatic temperature. This highlights the importance of considering individual-level traits when studying plant preference and creates a framework for assessing plant-pollinator networks. Future studies should examine additional traits that could explain the apparent size matching between unrestrictive flowers and proboscis.

Ecological interactions within a community shape the structure of ecosystems and influence ecosystem function. Plant-pollinator interactions exist as mutualistic exchange networks that may collapse as habitat loss occurs, thereby threatening the overall health of an ecosystem. Understanding the impacts of human-mediated habitat disturbance on ecological interactions is therefore crucial for conservation efforts. Archbold Biological Station (ABS) in Venus, Florida contains over 2000 hectares of protected Florida scrub habitat nested within a human-dominated environment that is threatened by anthropogenic habitat disturbance. In past studies, over 113 bee species and 157 associated host plants, many endemic to the Lake Wales Ridge, have been found on ABS property, providing an understanding of this system's plant-bee network. Using those data as a baseline, this study investigated the effects of varying levels of mechanical habitat disturbance intensity on the diversity and network structure of plant-bee interaction networks. Flowering plant abundance, richness, diversity, and composition as well as bee abundance and composition were significantly different across mechanical habitat disturbance levels. Interactions between bees and flowering plants also differed with varying disturbance intensity. From these results, it is clear that plants, bees and interactions between them are impacted by mechanical habitat disturbance in this system. This project informs management efforts not only for natural systems with the threat of alteration, but also for agricultural systems, many of which heavily rely on flower visitation by bee pollinators. This research also contributes to the growing field of interaction ecology by increasing understanding of habitat alteration effects on a valuable ecological interaction and ultimately ecosystem function.