Thesis (M.A.)--Texas State University-San Marcos, 2007. / Vita. Appendices: leaves 52-78. Includes bibliographical references (leaves 79-83).

Despite the variety of successful reports of the preservation, recovery, and identification of archaeological proteins in general, there are few positive reports regarding mass spectrometry-based identification of ceramic-bound proteins. In large part, this shortage is due to the lack of consideration for the unique taphonomic histories of such residues and, in general, methods development. Further, because negative results are rarely published, there is no baseline to which results can be compared. This paper attempts to address these challenges via a multi-pronged approach that uses mass spectrometry and complementary approaches to evaluate ceramic-bound protein preservation in both controlled, actualistic experiments, and in archaeological artifacts. By comparing the results obtained from protein-spiked, experimentally-aged ceramic to those obtained from both faunal and ceramic archaeological materials, an enhanced perspective on protein preservation and subsequent recovery and identification is revealed. This perspective, focusing on taphonomy, reveals why negative results may be the norm for ceramic artifacts when non-targeted methods are employed, and provides insight into how further method development may improve the likelihood of obtaining positive results.

No / The hair shaft has increasing importance in bioarchaeology, since it is now possible to retrieve detailed biomolecular information on recent life history using individual fibres (e.g., on diet, drug use and DNA). Data on hair condition is an important cornerstone to ensuring that reliable information is obtained. The following study defines morphological features of degradative change in human terminal scalp hair using different microscopy techniques. Evidence of degradative change is translated into a ranked histology for assessing hair sample condition. The approach is applied to samples of cut modern scalp hair subjected to degradation under soil burial/simulated grave conditions.

Post mortem interval (PMI – the time elapsed since death and discovery) is important to medicolegal investigations. It helps to construct crucial time lines and assists with the identification of unknown persons by inclusion or exclusion of a suspect’s known movements. Accurate methodologies for establishing PMI are limited to about 48-hours. Such methods involve use of increasing levels of potassium in vitreous humour, and algor mortis. This study is two-fold. Firstly, it explores the biomolecular changes in degrading porcine cartilage buried in soil environments and its potential to determine PMI in the crucial two days to two months period. Trotters were interred in a number of graves at two distinct locations exhibiting dissimilar soil environments. Weekly disinterments (for 6 weeks) resulted in dissection for cartilage samples which were processed for protein immunoblot analyses and cell vitality assays. Results demonstrate that aggrecan, a major structural proteoglycan, produces high (230kDa) and low (38kDa) molecular weight cross-reactive polypeptides (CRPs) within cartilage extracellular matrix. The 230kDa CRP degrades in a reproducible manner irrespective of the different soil environments utilised. As PMI increases, aggrecan diminishes and degrades forming heterogeneous subpopulations with time. Immunodetection of aggrecan ceases when joint exposure to the soil environment occurs. At this time, aggrecan is metabolised by soil microbes. The molecular breakdown of cartilage proteoglycans has potential for use as a reliable indicator of PMI, irrespective of differing soil environments, beyond the 48-hours period. Likewise, vitality assays also demonstrated viable chondrocytes for as long as 35 PM days. The second component of this study examined the fungal activity associated with trotters buried below ground. Results indicate that fungal growth was considerably influenced by soil chemistry and changes in the environment. Fungal colonisation did not demonstrate temporal patterns of succession. The results of this study indicate that cartilage has the potential to prolong PMI determination well beyond the current 48- and 100-hour limitations posed by various other soft tissue methods. Moreover, the long-term post mortem viability of chondrocytes presents an opportunity to explore DNA extraction from these cells for the purpose of establishing a positive identification for unidentified remains. On the contrary, the growth and colonisation patterns of post putrefactive fungi in relation to decomposing porcine trotters proved to be futile for estimating PMI. Therefore, fungi may not be a suitable candidate for evaluating PMI during the early phase fungal activity.

The hair shaft has increasing importance in bioarchaeology, since it is now possible to retrieve detailed biomolecular information on recent life history using individual fibres (e.g., on diet, drug use and DNA). Data on hair condition is an important cornerstone to ensuring that reliable information is obtained. The following study defines morphological features of degradative change in human terminal scalp hair using different microscopy techniques. Evidence of degradative change is translated into a ranked histology for assessing hair sample condition. The approach is applied to samples of cut modern scalp hair subjected to degradation under soil burial/simulated grave conditions.

A scarcity of experimental studies covering the decomposition of dismembered body parts has created a gap in knowledge of the effect of dismemberment on the estimation of post-mortem interval (PMI) and their post-mortem history in a forensic context. The aim of this study was to record the decay of detached body parts in some depositional settings where they are likely to be disposed of: burial, wrapping and freezing. A series of controlled laboratory experiments was carried out using Sus scrofa body parts and pork belly, to understand how ambient temperature, soil moisture, and wrapping and freezing of body parts affected their decomposition. Rates of decay were subject to a higher temperature and soil moisture level in a burial microenvironment, with metabolic microbial activity confirming the results. Temperature was a predominant factor in the decay rates of wrapped body parts, with a raised ambient temperature causing even higher temperature in the wrapped microenvironment, resulting in accelerated decay rates. Freezing decelerated the decomposition of body parts, retarding microbial growth and activity and causing differential decomposition between body parts. Freezing demonstrated morphological changes in body parts specific to this microenvironment. Predominantly Gram-negative bacteria that may be associated with body microflora were involved in decomposition in all three microenvironments. Taphonomic, chemical and microbiological analyses carried out in this study have a potential for forensic application in the examination of dismembered remains that have been deposited in freezing and indoor settings. Further experiments are necessary to understand buried decomposition patterns in field conditions.

The subject of corpses from mass violence is surprisingly unexplored, even though the materiality of the corpse carries strong symbolic capital in conflicts. The aim of my PhD research is to create new knowledge about the implications of unburied corpses that stem from intergroup conflicts, and subsequently to add knowledge concerning how intergroup violence is organised to achieve desired social agendas. In the licentiate thesis presented here, I research the conditions for postmortem agency and how treatment of corpses can be studied in prehistory, specifically through the material remains of unburied corpses from the Sandby borg massacre. The Sandby borg case study is explored through a bioarchaeological perspective. Inside the Iron Age ringfort, the remains of at least 26 individuals have been recovered hitherto. Several of the dead display traces of lethal intergroup violence. By integrating osteology, archaeology, taphonomy and social theories, I show how bioarchaeological research can contribute to the understanding of past postmortem agency in relation to intergroup violence as a social process. The thesis is comprised of four articles.