Orientador: Renato Mazzonetto / Dissertação (mestrado) - Universidade Estadual de Campinas, Faculdade de Odontologia de Piracicaba / Made available in DSpace on 2018-08-08T06:05:49Z (GMT). No. of bitstreams: 1 Kluppel_LeandroEduardo_M.pdf: 14531516 bytes, checksum: c01e828bcc790ef759e104126e1ff65a (MD5) Previous issue date: 2007 / Resumo: O objetivo deste estudo foi analisar histológica e radiograficamente a influência do tamanho das partículas da matriz óssea bovina anorgânica (MOBA) sobre o processo de reparação óssea. Na calvária de 18 coelhos adultos da raça Nova Zelândia foram preparadas quatro cavidades com diâmetro de 8 milímetros, sendo duas do lado direito da sutura sagital e duas do lado esquerdo. Os defeitos foram preenchidos com osso autógeno triturado (grupo controle); MOBA de granulação grossa; MOBA de granulação média ou MOBA de granulação fina. Os animais foram sacrificados nos períodos pós-operatórios de 15, 30 e 60 dias. Antes do início do processamento histológico, as peças foram radiografadas sequencialmente. Para análise destas imagens utilizou-se como padrão de comparação o osso do crânio que não estava envolvido nas áreas de ostectomia. Desta forma, observou-se que o osso autógeno apresentava-se discretamente radiopaco inicialmente, tendendo a apresentar uma radiopacidade bastante semelhante ao tecido adjacente no período final. A MOBA de granulação grossa e média mantiveram o mesmo padrão radiográfico, sendo que aos 60 dias, o aparecimento de uma porção radiolúcida em sua porção central pode ser observada. Já a MOBA de granulação fina apresentava discreta radiolucidez no período inicial, a qual tornou-se mais intensa nos períodos sucessivos. A análise histológica demonstrou a formação de maior quantidade de osso e menos reação inflamatória no grupo controle (osso autógeno). Para o biomaterial, em todas as granulações pôde-se observar a presença de infiltrado inflamatório considerável nos períodos de 15 e 30 dias. Nos defeitos preenchidos pela MOBA de granulações grossa e média o processo de reparação transcorreu de maneira semelhante, sendo que no período final uma grande quantidade de partículas e tecido conjuntivo fibroso ainda estavam presentes na cavidade. Contrariamente, a MOBA de granulação fina proporcionou a formação de maior quantidade de tecido osteóide e as partículas foram reabsorvidas quase que em sua totalidade transcorridos 60 dias de sua implantação. Com base nos resultados obtidos, conclui-se que: (1) o enxerto ósseo autógeno isoladamente proporcionou o melhor resultado em termos de reparação dos defeitos ósseos; (2) a MOBA é um material biocompatível; (3) a MOBA de granulação grossa e média não são reabsorvidas em sua totalidade no período observado; (4) a MOBA de granulação fina foi reabsorvida de forma mais intensa e proporcionou uma maior formação de tecido osteóide quando comparada às outras granulações / Abstract: The aim of this present study was to analyze, clinical and histologically, the influence of anorganic bovine bone matrix (ABBM) particle size on bone repair. Four calvarial defects of 8 millimeters each were prepared in 18 adult New Zealand rabbits, two in the right side, and two in the left side. The defects were filled with either particulate autogenous bone (control group); ABBM of large size granules; ABBM of medium size granules; or ABBM of small size granules. The animals were sacrificed at 15, 30, and 60 days after surgery. The samples were radiographic examined before being submitted to histological processing. The analysis of these radiographic images was performed by comparing them with images of the cranial bone not involved with the areas of osteotomy. Thus, it was observed that autogenous bone showed a slight radiopacity at the beginning which was increased at the final period, being very similar to the adjacent bone tissue in terms of radiopacity. The large and medium size ABBM particles maintained the same radiographic behavior, showing a radiolucid area in the central portion of the defect at 60 days. On the other hand, the ABBM of small size granules showed a slight radiolucity at the initial period, which was increased at the subsequent periods. The histological analysis showed a more intense bone formation within the control group (autogenous bone). With regards to the biomaterial, it was observed that all three particle sizes resulted in inflammatory infiltration at 15 and 30 days. The bone repair at the defects filled with ABBM of medium or large size granules was similar to each other, with the presence of a large amount of remaining particles and fibrous connective tissue in the defect at the final period. In contrast, ABBM of small size granules lead to a greater amount of osteoid tissue, and the particles were almost totally reabsorbed within 60 days of implantation. Based on these results, it was concluded that: (1) autogenous bone graft lead to the best result in terms of bone defect repair; (2) ABBM is a biocompatible material; (3) ABBM of large and medium size granules are not totally reabsorbed at the observed period; (4) ABBM of small size granules was more intensively reabsorbed, and lead to a greater osteoid tissue formation when compared to the medium and large ABBM granules / Mestrado / Cirurgia e Traumatologia Buco-Maxilo-Faciais / Mestre em Clínica Odontológica

Orientador: Roger William Fernandes Moreira / Dissertação (mestrado) - Universidade Estadual de Campinas, Faculdade de Odontologia de Piracicaba / Made available in DSpace on 2018-08-08T16:09:31Z (GMT). No. of bitstreams: 1 Sawazaki_Renato_M.pdf: 1407443 bytes, checksum: 22de8971b16885ec62214ad8fde67a9d (MD5) Previous issue date: 2007 / Resumo: O presente estudo tem por objetivo a avaliação dos tratamentos visando reconstrução dos maxilares com enxertos autógenos de crista ilíaca desenvolvidos na Faculdade de Odontologia de Piracicaba, Universidade de Campinas do período de primeiro de Janeiro de 2004 a trinta de junho de 2005. Foram avaliados 16 casos, nos quais se analisou dados extraídos da anamnese, exame físico e complementar, bem como indicação, planejamento, tratamento utilizado e proservação tanto da cirurgia para remoção de enxerto da crista ilíaca quanto da cirurgia para instalação de implantes. Um questionário foi usado para coletar dados relativos ao período pós-operatório da cirurgia da crista ilíaca, como dor, padrão de cicatrização, distúrbios sensoriais e motores, resolução do tratamento e seqüelas; e dados relativos à satisfação do paciente frente às dificuldades, sucessos e insucessos possíveis. A dor pós-operatória foi referida em 100% dos pacientes submetidos ao enxerto, 93,5% de distúrbio de marcha, 25% de queixa quanto à cicatriz e 12,5% de perda de enxerto. Os resultados sugerem, também, relação entre maior dor pós-operatória no sítio doador em pacientes com índices de massa corpórea acima do normal. Quanto à cirurgia de implantes foi constada falha precoce em 2,0% dos 98 implantes instalados. Assim, a reconstrução de maxilares é um procedimento seguro com altas taxas de sucesso devendo ser usada sempre que necessário / Abstract: The aim of the present study is to evaluate treatment outcomes for reconstructive surgery of the jaws with iliac crest as donnor site performed at Piracicaba Dental School, UNICAMP, between the begin of january 2004 and the end of june 2005. Sixteen cases were selected and data obtained from the clinical records were analysed. This data included medical history, physical examination, treatment indications and planning as well as follow up observations for the donnor site and dental implants installed in the grafted area. A questionnaire was applied to evaluate data relative to postoperative pain, healing pattern, motor and neurosensorial disturbances, treatment outcomes and overall satisfaction. Data analysis related to the graft procedure revealed postoperative pain in 100% of the patients, gait disturbances in 93.5%, visible scar in 25% and graft loss in 12.5%. Related to implant surgery, it was observed early loss in 2.0% of the 98 implants installed. The results also suggest a relationship between postoperative higher scores of pain in the donor site in elevated body mass indexes patients. Therefore, maxillary reconstruction is safe procedure with elevated success rates / Mestrado / Cirurgia e Traumatologia Buco-Maxilo-Faciais / Mestre em Clínica Odontológica

Conselho Nacional de Desenvolvimento CientÃfico e TecnolÃgico / Foi estudada a interaÃÃo enxerto/porta-enxerto nas caracterÃsticas morfolÃgicas e produtivas de plantas de cajueiro anÃo nas fases de produÃÃo das mudas e cultivo no campo sob regime de sequeiro na Fazenda Planalto (CIONE), localizada no municÃpio piauiense Pio IX, semi-Ãrido nordestino. Em novembro de 2004 foram semeados os porta-enxertos CP 76, CP 06, CP 09, BRS 226, CAC 38 e CAC 40 avaliando as variÃveis emergÃncia de plÃntulas e vigor do porta-enxerto antes da enxertia, que ocorreu em janeiro de 2005, utilizando os clones CCP 76 e BRS 226. Transcorridos 60 dias da enxertia, as variÃveis rendimento da enxertia e vigor das plantas foram analisadas e, em seguida, transplantadas sob DBC em esquema fatorial 6 x 2 com quatro repetiÃÃes e nove plantas por parcela, espaÃamento 8 m entre linhas e 6 m entre plantas. As variÃveis desenvolvimento morfolÃgico e produÃÃo total foram avaliadas anualmente entre 2005 e 2008 enquanto caracterizaÃÃo de castanha e pedicelo na Ãltima safra. Os dados foram submetidos à anÃlise de variÃncia e comparados pelo teste de mÃdias. O uso de diferentes porta-enxertos afetou o porte das plantas e a produtividade de castanhas e pseudofrutos de cajueiro anÃo precoce, sem, contudo, afetar o tamanho e a massa das castanhas e amÃndoas produzidas. As combinaÃÃes BRS 226/CAC 38 e BRS 226/CP 09 sÃo consideradas as mais promissoras / We studied the interaction between stock and rootstock in the morphological and productive characteristics in dwarf cashew trees, in the stages of runner plant production and cultivation on the field, under unirrigated conditions. The experiment took place in the Planalto Farm (CIONE), located in Pio IX-Piaui, in the northeast. In November of 2004 we planted the rootstocks CP 76, CP 06, CP 09, BRS 226, CAC 38 and CAC 40, when we evaluated the seedling emergence and rootstock vigor before grafting (in January of 2005) using the clones CCP 76 e BRS 226. Sixty days after grafting, we analyzed the seedling yield and plant vigor and afterwards we transplanted them under DBC in factorial scheme (6 x 2) with four repetitions and nine plants per plot, spacing 8 m between lines and 6 m between plants. The variables of morphological development and total production were evaluated annually from 2005 to 2008, observing the characteristics of cashew nuts and pedicels from the last crop. The data was used in the analysis of variance and then compared using the means test. The use of different rootstocks affected the plant sizes and nut production and pseudofruits in early dwarf cashew trees, however it did not affect the size and the mass of cashew nuts and kernels produced. The most promising combinations are BRS 226/CAC 38 e BRS 226/CP 09

Orientador: Maria Beatriz Puzzi, Maria Helena Stangler Kraemer / Dissertação (mestrado) - Universidade Estadual de Campinas, Faculdade de Ciencias Medicas / Made available in DSpace on 2018-08-04T03:54:34Z (GMT). No. of bitstreams: 1 Souto_LuisRicardoMartinhao_M.pdf: 2402921 bytes, checksum: a79b6ae181ce1b24d01ec608815d8bf7 (MD5) Previous issue date: 2005 / Resumo: A obtenção de uma pele humana que apresente derme e epiderme, reconstruída a partir de células isoladas de pacientes, possibilita a realização de enxertos autólogos de pele reconstruída em laboratório (in vitro) em pacientes com áreas doadoras escassas além de permitir ensaios com substâncias químicas e drogas in vitro e não mais in vivo. A partir da cultura de fibroblastos humanos, é possível obter um número suficiente de células que podem ser injetadas em uma matriz de colágeno bovino tipo I que, mantida imersa em meio de cultura, específico para fibroblastos, permite a formação de uma derme humana reconstruída in vitro. Sobre essa derme, através de cultura de queratinócitos e melanócitos humanos, forma-se uma epiderme diferenciada levando à formação de uma pele humana reconstruída in vitro, constituída de derme e epiderme associadas. Essa pele humana formada é, histologicamente, semelhante à pele humana in vivo. Na derme, identifica-se o tecido colágeno, com suas células, e a matriz extracelular organizados paralelamente à epiderme. Esta se desenvolve em várias camadas. Não há distinção entre derme e epiderme no experimento controle, onde não foi utilizado o colágeno bovino tipo I / Abstract: The technique to obtain human skin presenting dermis and epidermis reconstructed from cells isolated from patients allows the performance of autologous grafts of skin reconstructed in laboratory (in vitro) on patients with scarce donor sites, in addition to permitting trials with chemical substances and drugs no more in vivo, but in vitro. It is possible to obtain a sufficient number of cells from human fibroblast culture that can be injected in bovine collagen type I matrix and kept submerged in a specific culture medium for fibroblasts. This will permit the formation of human dermis reconstructed in vitro. On this dermis, through culture of human keratinocytes and melanocytes, a differentiated epidermis is formed, leading to the creation of human skin reconstructed in vitro, composed of associated dermis and epidermis. This human skin is histologically formed in the same way as human skin in vivo. Collagen tissue can be identified in the dermis, with its cells and extracellular matrix organized in parallel to the epidermis, which is developed in several layers / Mestrado / Patologia Clinica / Mestre em Ciências Médicas

Blends of poly(vinylidene fluoride) (PVDF) and polyamide 6 (N6) are interesting for both scientific studies and commercial exploitation. PVDF is known to be miscible with polymers produced from monomers containing carbonyl side groups, eg. polyethyl acrylate, polyacrylamide etc., but is not miscible with polymers containing carbonyl groups in the main chain, ego polyamides, polyester, etc. Although complete miscibility of the blend components is not always necessary, strong physical interactions between the two components are needed in order to obtain a compatible blend, i.e. one which exhibits good mechanical properties. An investigation was carried out to explore the possibility to compatibilise blends of PVDF and N6 using y-radiation to graft acid groups on either polymer and subsequently ionomerizing these with zinc cations. Graft copolymer type of compatibilizer was produced when the acid functionalized PVDF (grafted PVDF) was blended with N6 or acid functionalized N6 (grafted N6). Fourier transform infrared analysis has confirmed the occurance of reactions between acid groups in the grafted PVDFand amine groups in the N6. The compatibility of the PVDF/N6 blends was found to increase with increasing amount of carboxylic acid groups in the two polymers. This was accompanied by an increase in Tg of the N6 phase in blend, which became more pronounced when both components were grafted. Tensile test and solvent resistance experiments were carried out to relate the compatibization of the blend to the improvement in mechanical properties. Ionomerization of the functionalized polymers with zinc cations was performed in order to study the effect on compatibility of the blend. The neutralization of the acid groups in either polymer in the blend by addition of zinc acetyl acetonate was found to suppress the chemical reaction with the amine end groups in the N6 phase, and to cause a reduction in the T g and a reduction in crosslinking of the N 6 phase. However when both polymers were grafted, the crystallinity of the N6 phase was restored, which was accompanied by an increase in Tg.

Abstract A new animal model was developed to evaluate the effect of bovine native bone morphogenetic protein (BMP) on the healing of segmental, critical-sized bone defects. Laboratory-bred adult beagle dogs were used in the study. A 2 cm corticoperiosteal defect was created using an oscillating saw in mid-ulna, and the defect was treated with bone grafts and implants fixed by an intramedullary Kirschner wire through predrilled holes in the middle of the implant. Plate and screw fixation was also used in some groups. Coral, hydroxyapatite and demineralized xenograft bone were placed in the defects with or without BMP. Autografts and allografts were used as controls. The BMP was extracted from bovine diaphyseal bone. The follow-up period was 36 weeks. Radiographs were taken at regular intervals during the follow-up period, and bone formation and bone union were evaluated. The radiographs were digitized, and callus was measured and CT scans obtained to define bone density. At the end of the study, the bones were harvested and tested mechanically in a torsion machine until failure. After mechanical testing, the bones were reconstructed and histological sections were made. With autograft and allograft bone grafts, healing was nearly complete. Hydroxyapatite and demineralized xenograft bone did not result in healing of the bone defect, while coral enhanced bone formation, but the healing was not comparable to autografts or allografts. Hydroxyapatite implants did not resorb during the 36 weeks of follow-up to enhance bone healing, and there was a fibrous capsule around the hydroxyapatite implants in histology. Xenograft bone was resorbed, and very little bone formation and extensive fibrosis were seen at the implant site. Coral was resorbed and gradually replaced by new bone, but did not heal the defect completely. With every implant, added BMP had a positive effect on healing as evaluated either radiographically, mechanically or histologically. Coral was the most optimal carrier material for BMP among the materials tested in this study. The animal model seems to be suitable for studying the healing of bone defects, as all the animals were physically active from the first postoperative day and did not seem to have problems with motion during the follow-up period. Intramedullary fixation lacks rotational stability, which may have a negative effect on healing. The bones fixed with a plate and screws showed better scores in radiographs and were mechanically stronger, although the study groups were too small to allow definitive conclusions. As a conclusion, none of the transplants or implants were equally efficient as cortical autograft in healing segmental ulnar defects. BMP did not enhance the poor capacity of hydroxyapatite and xenograft bone to heal the bone defect. According to the present findings, the composite implant consisting of coral and BMP seemed to be the best of the composite implants tested.

京都大学 / 0048 / 新制・課程博士 / 博士(農学) / 甲第22497号 / 農博第2401号 / 新制||農||1077(附属図書館) / 学位論文||R2||N5277(農学部図書室) / 京都大学大学院農学研究科森林科学専攻 / (主査)教授 矢野 浩之, 教授 和田 昌久, 教授 辻井 敬亘 / 学位規則第4条第1項該当 / Doctor of Agricultural Science / Kyoto University / DFAM

The diploma thesis deals with post-polymerization hydroxylation of polypropylene in solid state. Regarding the literature review, polypropylene was hydroxylated by radical grafting in aqueous solution of potassium persulfate at 100 °C, under nitrogen atmosphere for 60 minutes. Hydroxylation of polypropylene was performed at different concentrations of potassium persulfate (1; 5; 10 mol. %) and two different water/potassium persulfate molar ratios. The effects of reaction system composition and reaction conditions on reaction efficiency, extent of side reactions, thermal and rheological properties of hydroxylated polypropylene were evaluated. The presence and concentration of hydroxyl groups on polypropylene surface was determined by structural analysis (FTIR, XPS), while the highest efficiency was achieved in the presence of nonionic wetting agents, using 10 mol. % potassium persulfate and at lower water/potassium persulfate molar ratio. Based on changes in polypropylene structure, the modification took place mainly in the amorphous phase of the polymer. In addition to hydroxylation, concurrent side reactions have been reported, in particular the oxidation of wetting agents and polypropylene, which has resulted in chain cleavage, reducing the average molecular weight of the polypropylene.

Nowadays, catalysis lies at the heart of economy growth mainly in the petroleum industry. Catalysis can offer real and potential solutions to the current challenges for a long-term sustainable energy, green chemistry, and environmental protection. In this context, one of the most important and future prosperity promising catalytic applications in the petrochemical field is hydrocarbons metathesis; it consists on the conversion of both renewable and non-petroleum fossil carbon sources to transportation fuels. Olefin metathesis has become one of the standard methodologies for constructing C-C bonds in many organic transformation reactions. This owed to the numerous types of metathesis reactions that have been developed, for example, enyne, ring-opening and closing, self and cross metathesis, etc. But the one step conversion of olefin to alkanes has not been studied much. Recently, only one such a work has been published for the hydro-metathesis of propylene by tantalum hydride supported on KCC-1 in dynamic reactor. With this knowledge, we thought to study the hydro-metathesis using liquid olefin (1-decene). Another aspect of using 1-decene comes from our previous experience on metathesis of n-decane where the first step is the conversion of decane to 1-decene and subsequently to different chain length alkanes with W-alkyl/alkylidene catalyst. In this way, it would be easy for us to use different catalysts and compare them with parent catalyst concerning TON. We found 100% conversion with TON of 1010 using supported WMe6 onto SiO2-700 [(≡Si-O-)WMe5] against the previous results for n-decane showing 20% conversion and TON of 153. In this work, we disclose the hydro-metathesis reaction of 1-decene using well-defined silica supported W(VI), Mo(VI) and Ta(V) alkyl catalysts in batch reactor condition. This work is divided into three major sections; first chapter contains an introduction to the field of catalysis and surface organometallic chemistry. In second chapter, we describe all the experimental procedures of the catalysts. The third chapter is devoted to the characterization and interpretation followed by catalytic reactions. Finally, a brief conclusion of the present study is given.

Design of a new well-defined surface organometallic species [O-(=Si–NH)2Zr(IV)Np2] has been obtained by reaction of tetraneopentyl zirconium (ZrNp4) on SBA-15 surface displaying mainly silylamine pairs [O-(=Si–NH2)2]. These surface species have been achieved by an ammonia treatment of a highly dehydroxylated SBA-15 at 1000°C (SBA-151000). This support is known to contain mainly strained reactive siloxane bridges (≡Si-O-Si≡)[1] along with a small amount of isolated plus germinal silanols =Si(OH)2. Chemisorption of ammonia occurs primarily by opening these siloxane bridges[2] to generate silanol/silylamine pairs [O-(=Si–NH2)(=SiOH)] followed by substitution of the remaining silanol. Further treatment using hexamethyldisilazane (HMDS) results in the protection of the isolated remaining silanol groups by formation of ≡Si-O-SiMe3 and =Si(OSiMe3)2 but leaves ≡SiNH2 untouched. After reaction of this functionalized surface with ZrNp4, this latter displays mainly a bi-podal zirconium neopentyl organometallic complex [O-(=Si–NH)2Zr(IV)Np2] which has been fully characterized by diverse methods such as infrared transmission spectroscopy, magic angle spinning solid state nuclear magnetic resonance, surface elemental analysis, small angle X-ray powder diffraction (XRD), nitrogen adsorption and energy filtered transmission electron microscopy (EFTEM). These different characterization tools unambiguously prove that the zirconium organometallic complex reacts mostly with silylamine pairs to give a bi-podal zirconium bis-neopentyl complex, uniformly distributed into the channels of SBA-151000. Therefore this new material opens a new promising research area in Surface Organometallic Chemistry which, so far, was dealing mainly with O containing surface. It is expected that vicinal amine functions may play a very different role as compared with classical inorganic supports. Given the importance in the last decades of N containing ligands in catalysis, one may expect important prospects…