Thesis (MScAgric) -- University of Stellenbosch, 2000. / ENGLISH ABSTRACT: Postharvest berry split and abscission are prevailing physiological disorders that negatively impact on the quality of table grapes exported from South Africa. Inferior grape quality due to these disorders results in a considerable decline in consumer confidence in the branded product, which leads to a drop in demand, and consequently, lower prices. Since information concerning postharvest factors influencing postharvest berry split and abscission is limited, the search for reliable methods to adequately control these problems remains elusive. In an attempt to obtain the required information, the influence of harvest temperature, harvest maturity, perforated liners, field heat removal prior to packing, delay periods before and after packing, storage duration and the elevation of storage temperature on the development of berry split and abscission in 'Thompson Seedless' (Vitis vinifera Linnaeus) table grapes was investigated. Changes in abscission related factors during berry development, and the influence of pre-and postharvest ethylene inhibitors on the development of berry abscission in 'Waltham Cross' table grapes, was also studied. Berry split was aggravated by packing 'Thompson Seedless' grapes at high pulp temperatures of approximately 30°C, especially if the grapes were packed in non-perforated bags. The incidence of berry split could be reduced by between 80 and 90% by packing grapes in perforated instead of non-perforated liners. Perforated bags also reduced levels of S02 damage. However, due to significantly more moisture loss from grapes in perforated bags, compared to non-perforated bags, the risk of higher fruit and stem desiccation and softer berries existed. Optimum size and density of perforations needs to be determined to reduce berry split without excessive loss of moisture from the grapes, and S02gas from the air space surrounding the product. The influence of harvest temperature and liner type on berry abscission was not conclusive. Advanced maturity increased grape resistance to berry split. However, grapes harvested too mature were prone to stem desiccation and the development of Botrytis decay. The occurrence of berry abscission also appeared to increase with advanced harvest maturity. Consequently, to ensure optimal post-storage quality, 'Thompson Seedless' grapes should be harvested as soon as horticultural maturity has been reached, which appears to be at approximately 18°Brix. Field heat removal for 1.5 hours at 19°C prior to packing had no beneficial or adverse effect on berry split and abscission. Delay periods prior to packing aggravated berry abscission, but did not influence berry split significantly. Grapes delayed for 12 hours showed a significant increase in berry abscission and Botrytis decay, compared to grapes delayed for only 3 or 8 hours. Considering that the absence of fungal decay is the most important quality prerequisite in table grapes, it is of vital importance to pack grapes with as short a delay period as possible. Grapes packed in non-perforated liners and delayed for different durations after packing, before the onset of forced-air cooling (FAC), showed significant differences regarding the incidence of berry split. Grapes delayed for 18 hours had significantly higher levels of berry split directly after the delay period, compared to grapes delayed for 6 or 12 hours. No significant difference in berry abscission occurred between grapes delayed for different periods. To minimise the amount of berry split, FAC should be applied as rapidly as possible after the packing of grapes in non-perforated liners. Two storage related factors significantly influenced the incidence of berry split in 'Thompson Seedless' grapes during cold storage significantly, viz. the duration of storage at -O.soC,and the increase in temperature after low temperature storage. Berry split increased almost linearly with prolonged storage at -O.soC. An elevation of storage temperature from -O.soC to 10°C any time during the cold storage period, further aggravated the split problem. Consequently, the reduction of berry split in 'Thompson Seedless' table grapes during cold storage requires (a) the shortest possible cold storage period, and (b) good temperature management throughout distribution, from initiation of cooling until the final point of sale. The grape berry abscission potential, as quantitatively indexed by the measurement of the fruit removal force (FRF), showed significant changes during berry development of 'Waltham Cross' table grapes, from 27 to 111 days after full bloom (OAFB). This showed that at certain stages of fruit growth, 'Waltham Cross' grapes are more prone to berry abscission. At 27 OAFB, when the berries had an average diameter of 6.6mm, the grape bunches showed a significantly higher potential for berry abscission, compared to grapes sampled at a later stage. 'Waltham Cross' has inherently straggly bunches with bare shoulders. Therefore, any abscission during berry development will aggravate the problem. Consequently, it is of vital importance that any adverse factors such as moisture stress be avoided, especially during the period when 'Waltham Cross' grapes appear to be very susceptible to berry abscission. Of all parameters measured, moisture loss showed the best correlation with abscission. Grapes harvested with total soluble solids (TSS) of 12.3°Brix, 83 OAFB, had a significantly higher abscission potential than grapes harvested more mature. Therefore, by harvesting 'Waltham Cross' grapes at optimum maturity, at a TSS of approximately 16.4°Brix, berry abscission can be reduced to a great extent. It was evident that at veraison, the metabolism of grape berries changes drastically, and additional to the rapid increase in sugars and the rapid decrease in acidity, a decrease in FRF occurs. Preharvest sprays of ReTain™ (a derivative of aminoethoxyvinylglycine), which inhibits ethylene synthesis, showed no promise as a means to reduce postharvest berry abscission. A postharvest treatment with EthylBloc® (1-methylcyclopropene), which inhibits ethylene action, only reduced berry abscission during one season. / AFRIKAANSE OPSOMMING: Die fisiologiese defekte korrelbars en los korrels wat algemeen voorkom tydens opberging van sekere tafeldruif-kultivars, het 'n negatiewe invloed op tafeldruiwe wat uitgevoer word vanaf Suid-Afrika. Minderwaardige kwaliteit as gevolg van hierdie defekte het 'n aansienlike afname in verbruikers-vertroue tot gevolg wat aanleiding gee tot 'n ooreenkomstige afname in aanvraag en prys van die produk. Inligting rakende na-oes faktore wat die voorkoms van korrelbars en los korrels beïnvloed is beperk, en geen gewaarborgde metode bestaan om hierdie twee defekte volkome te beheer nie. In 'n poging om dié gewenste inligting te bekom, is ondersoek ingestel na die effek van oes-temperatuur, oes-rypheid, geperforeerde sakke, veldhitte verwydering voor verpakking, vertragingsperiodes voor en na verpakking, tydsduur van opberging, en die verhoging van die opbergingstemperatuur, op die voorkoms van korrelbars en los korrels by 'Thompson Seedless' (Vitis vinifera Linnaeus) druiwe. Daar is ook ondersoek ingestel na veranderings in afsnoering verwante faktore tydens korrel-ontwikkeling, en die invloed van vooren na-oes toedienings van etileen inhibeerders op die ontwikkeling van los korrels by 'Waltham Cross'tafeldruiwe. Korrelbars is vererger deur 'Thompson Seedless' met hoë pulptemperature van ongeveer 29.5°C te verpak, veral indien dit in 'n riie-geperforeerde sak verpak is. Die voorkoms van korrelbars kon tussen 80 en 90% verminder word deur 'Thompson Seedless' druiwe in geperforeerde sakke te verpak, in plaas van nie-geperforeerde sakke. Geperforeerde sakke het ook S02 skade op die druiwe verminder. Tog, as gevolg van betekenisvol meer vogverlies vanaf druiwe in geperforeerde sakke as vanaf druiwe in nie-geperforeerde sakke, bestaan die risiko van meer stingel-uitdroging en minder ferm korrels indien druiwe in geperforeerde sakke verpak word. Optimale grootte en digtheid van perforasies moet bepaal word om korrelbars te verminder, maar sonder oormatige vogverlies vanaf die druiwe en oormatige verlies aan S02. Die invloed van oes-temperatuur en sak-tipe op los korrels was nie oortuigend nie. Gevorderde oes-rypheid het die druif se weerstand teen korrelbars verhoog. Daarteenoor was druiwe wat té ryp geoes is, meer gevoelig vir stingel-uitdroging en Botrytis bederf. Dit wilook voorkom of die voorkoms van los korrels toeneem met gevorderde rypheid. Dus, om optimum kwaliteit na opberging te verseker, moet 'Thompson Seedless' geoes word sodra hortologiese rypheid bereik word, wat blyk om by 'n totale opgeloste vaste stof-inhoud (TOVS) van ongeveer 18°Brix te wees. Veldhitte verwydering voor verpakking, vir 1.5 uur by 19°C, het geen effek gehad op die voorkoms van korrelbars en los korrels nie. 'n Vertragingsperiode voor verpakking het die los korrel-probleem vererger, alhoewel dit geen betekenisvolle invloed op die voorkoms van korrelbars gehad het nie. Druiwe wat vir 12 uur voor verpakking vertraag is, het betekenisvol meer los korrels en Botrytis bederf getoon, in vergelyking met druiwe wat slegs 'n vertragingsperiode van 3 of 8 uur ondergaan het. Aangesien die afwesigheid van bederf die belangrikste kwaliteits-vereiste vir tafeldruiwe is, is dit van kardinale belang om druiwe so gou as moontlik na oes te verpak. Druiwe, verpak in nie-geperforeerde sakke, wat vir verskillende periodes vertraag is voor geforseerde-lug verkoeling, het betekenisvolle verskille getoon betreffende die voorkoms van korrelbars. Druiwe vertraag vir 18 ure voor verkoeling, het betekenisvol meer korrelbars getoon, soos gemeet onmiddellik na die vertragingsperiode, in vergelyking met druiwe wat slegs vir 6 of 12 ure vertraag was. Geen betekenisvolle verskille in los korrels het voorgekom tussen druiwe wat verskillende vertragingsperiodes ondergaan het nie. Om korrelbars te verminder, moet geforseerde-lug verkoeling so gou as moontlik na verpakking van druiwe in nie-geperforeerde sakke toegepas word. Twee opbergings-verwante faktore beïnvloed die voorkoms van korrelbars by 'Thompson Seedless' druiwe tydens koelopberging, naamlik die tydsduur van opberging by -O.soC,asook 'n styging in temperatuur vanaf -O.soC tot 1DoC. Korrelbars het feitlik liniêr toegeneem met verlengde opberging by -O.soC. 'n Styging in temperatuur vanaf -O.SoCtot 1DoCop enige tydstip gedurende die koelopbergingsperiode, het korrelbars verder vererger. Dus, om korrelbars by 'Thompson Seedless' tydens opberging tot die minimum te beperk, moet die tydsduur van opberging so kort as moontlik wees, en moet die koue ketting regdeur die distribusie-proses gehandhaaf word, vanaf inisiëring van verkoeling tot en met die uiteindelike verkoop van die produk. Die afsnoerings-potensiaal van druiwe, soos kwantitatief geïndekseer is deur meting van die vrug-verwyderings-vermoë (VVV), het betekenisvol verander gedurende korrel-ontwikkeling van 'Waltham Cross' tafeldruiwe, vanaf 27 tot 111 dae na volblom (DNVB). Dit het getoon dat 'Waltham Cross' druiwe by sekere stadiums van vrug-groei meer gevoelig is vir korrel afsnoering. By 27 DNVB, wanneer die korrels 'n gemiddelde deursnee van 6.6mm gehad het, het die druiwe 'n betekenisvolle hoër potensiaal vir afsnoering getoon, in vergelyking met druiwe wat op 'n latere stadium getoets is. 'Waltham Cross' is inherent geneig tot yl trosse met kaal skouers, gevolglik sal enige afsnoering tydens korrel-ontwikkeling die probleem vererger. Dus is dit van kardinale belang dat enige nadelige faktor, soos byvoorbeeld vogstres, vermy moet word, veral gedurende periodes wanneer dit wil voorkom of 'Waltham Cross' baie vatbaar is vir korrel afsnoering. Van al die parameters wat gemeet is, het vogverlies die beste korrelasie met korrel afsnoering getoon. Druiwe wat 83 DNVB, by 'n TOVS van 12.3°Brix geoes is, het 'n betekenisvol hoër potensiaal vir korrel afsnoering getoon, in vergelyking met druiwe wat ryper geoes is. Dus, deur 'Waltham Cross' druiwe by optimum rypheid te oes, by 'n TOVS van ongeveer 16.4°Brix, kan korrelbars in 'n groot mate verminder word. Tydens verelson, wanneer die metabolisme van die druiwe drasties verander, was daar gepaardgaande met die drastiese toename in TOVS en die drastiese afname in totale titreerbare sure (TSS), ook 'n afname in Voor-oes bespuitings met ReTain™, wat etileen sintese inhibeer, het geen potensiaal getoon om los korrels by 'Waltham Cross' te verminder nie. 'n Na-oes behandeling met EthyIBloc®, wat etileen werking inhibeer, het slegs korrel afsnoering in een van die seisoene effens verminder.

Thesis (MScAgric)--University of Stellenbosch, 2004. / ENGLISH ABSTRACT: Due to the extraordinary drought resistance of the grapevine, viticulture without irrigation in the winter rainfall coastal areas of South Africa is a feasible and commonly used practice. Wine quality is largely determined by the quality of the grapes from which it is made. Grapevine physiology is affected both directly and indirectly by water stress, which may vary according to soil type and prevailing atmospheric conditions. The water status of the grapevine can affect grape composition profoundly, either directly or indirectly, in either a positive or negative way, depending on the degree as well as the duration of water stress. There are three important factors involved in the development of water stress, namely the transpiration rate, the rate of water movement from the soil to the roots, and the relationship of soil water potential to leaf water potential. All three these factors are affected by atmospheric and/or soil conditions. In warm winelands such as South Africa (Western Cape), with a mediterranean climate which is characterised by a hot, dry summer period, the most important characteristic of soil is its ability to supply sufficient water to the grapevine during the entire growing season. Leaf water potential (Ψl) has gained wide acceptance as a fundamental measure of grapevine water status, and has been widely applied in viticultural research. Shortly before dawn, Ψl approaches equilibrium with soil water potential and reaches a maximum daily value. The study formed an integral part of a comprehensive, multi-disciplinary research project (ARC Infruitec-Nietvoorbij Project No. WW13/01) on the effect of soil and climate on wine quality, which commenced in 1993 and will be completed in 2004. This study was conducted during the 2002/03 growing season in two Sauvignon blanc vineyards situated at Helshoogte and Papegaaiberg, both in the Stellenbosch district, approximately nine kilometres apart. Two experiment plots, representing contrasting soil types in terms of soil water regime, were selected in each vineyard. At Helshoogte the two soils represented the Tukulu and Hutton forms, and the soils at Papegaaiberg were of the Avalon and Tukulu forms. The aim of this study was to determine the effect of atmospheric conditions and soil water status on the level of water stress in the grapevines for each soil at each locality, as well as the effect of grapevine water stress on yield and wine quality. This was done by determining and comparing the soil water status, soil water holding capacity of the soils and the evapotranspiration of the grapevines on the two different soils, at each of the two localities differing in mesoclimate and topography. The atmospheric conditions at the two localities during the 2002/03 season were also determined and compared to the long-term average atmospheric conditions, and the level of water stress of grapevines on each soil at each locality was measured. During the 2002/03 growing season, atmospheric conditions were relatively warm and dry in comparison to the long-term averages of previous seasons. These conditions accentuated the effects of certain soil properties that may not come forward during wetter, normal seasons. The usually wet Tukulu soil at Helshoogte was drier than expected during the 2002/03 season compared to the Hutton soil. Due to more vigorous growth on the Tukulu soil, grapevines extracted more soil water early in the season, leading to a low soil water matric potential and more water stress in the grapevines. Due to the higher vigour, resulting in more canopy shading, and more water stress, the dominant aroma in wines from the Tukulu soil was fresh vegetative. The Hutton soil maintained consistency with regards to both yield and wine quality compared to previous seasons. On the other hand the Tukulu soil supported a higher yield, but with lower than normal wine quality. The Avalon soil at Papegaaiberg maintained the highest soil water potential towards the end of the season, probably due to capillary supplementation from the sub-soil. Grapevines on the Tukulu soil at Papegaaiberg experienced much higher water stress than ones on the other three soils, especially during the later part of the season. This could be ascribed to a combination of factors, the most important being the severe soil compaction at a shallow depth, seriously limiting rooting depth and root distribution, which is detrimental to grapevine performance. Both the soil water status and atmospheric conditions played important roles in determining the amount of water stress that the grapevines experienced at different stages. The air temperature and vapour pressure deficit throughout the season were consistently lower at Helshoogte, the cooler terroir, compared to Papegaaiberg, the warmer terroir. At flowering, Ψl was lower for grapevines at Helshoogte than at Papegaaiberg, showing that diurnal grapevine water status was primarily controlled by soil water content. The difference in grapevine water status between the two terroirs gradually diminished until it was reversed during the post harvest period when Ψl in grapevines at Papegaaiberg tended to be lower compared to those at Helshoogte. The relatively low pre-dawn Ψl at Helshoogte indicated that the grapevines were subjected to excessive water stress resulting from the low soil water content. However, grapevines at Helshoogte did not suffer material water stress (i.e. Ψl < -1.20 MPa) during the warmest part of the day, suggesting that partial stomatal closure prevented the development of excessive water stress in the grapevines. This suggests that low pre-dawn Ψl values do not necessarily imply that grapevines will experience more water stress over the warmer part of the day, or visa versa. This does not rule out the possibility that side-effects of partial stomatal closure, such as reduced photosynthesis, can have negative effects on grapevine functioning in general. These results also suggest that measurement of diurnal Ψl cycles at various phenological stages is required to understand and quantify terroir effects on grapevine water status. / AFRIKAANSE OPSOMMING: Danksy die droogteweerstand van die wingerdstok is die verbouing van wingerde sonder besproeiing ‘n praktiese en algemene verskynsel in die winterreënval-areas van Suid-Afrika. Wynkwaliteit word grootliks bepaal deur die kwaliteit van die druiwe waarvan dit gemaak word. Wingerdfisiologie word direk en indirek beïnvloed deur waterstres, wat kan varieer volgens die grondtipe en die heersende atmosferiese toestande. Die waterstatus van die wingerdstok beïnvloed druifsamestelling, direk of indirek, en positief of negatief, afhangend van die graad en tydsduur van die waterstres. Daar is drie belangrike faktore betrokke by die ontwikkeling van waterstres, naamlik die transpirasietempo, die tempo van waterbeweging vanaf die grond na die wortels, en die verhouding tussen die grondwatermatrikspotensiaal tot blaarwaterpotensiaal. Al drie die faktore word beïnvloed deur die atmosferiese en/of grondtoestande. In warm wynboulande soos Suid-Afrika (Weskaap), met ‘n meditereense klimaat wat gekarakteriseer word deur ‘n warm, droë somerperiode, is die belangrikste eienskap van grond die vermoë om voldoende water aan die wingerdstok te verskaf gedurende die hele seisoen. Blaarwaterpotensiaal (Ψl) het wye aanvaarding bekom as die fundamentele meting van wingerstokwaterstatus, en word wyd toegepas in wingerdkundige navorsing. Kort voor sonsopkoms, nader Ψl ‘n ewewig met die grondwatermatrikspotensiaal en bereik ‘n maksimum daaglikse waarde. Die studie vorm ‘n integrale deel van ‘n omvattende, multi-dissiplinêre navorsingsprojek (ARC Infruitec-Nietvoorbij Projek No. WW13/01) op die effek van grond en klimaat op wynkwaliteit, wat in 1993 in aanvang geneem het en in 2004 afgehandel sal word. Hierdie studie is uitgevoer gedurende die 2002/03 seisoen in twee Sauvignon blanc wingerde geleë by Helshoogte en Papegaaiberg, beide in die Stellenbosch distrik, ongeveer nege kilometer van mekaar. Twee eksperimentele persele, elkeen verteenwoordigend van kontrasterende grondtipes in terme van grondwaterregime, is geselekteer in elke wingerd. By Helshoogte word die twee gronde verteenwoordig deur die Tukulu en Hutton grondvorms, en die gronde by Papegaaiberg is van die Avalon en Tukulu vorms. Die doel van die studie was om die effek van atmosferiese toestande en grondwaterstatus op die wingerdstok se waterstatus vir elke grond by die twee lokaliteite te bepaal, sowel as die effek van die wingerdstok se waterstatus op die opbrengs en wynkwaliteit. Dit is gedoen deur die grondwaterstatus, die grondwaterhouvermoë, sowel as die evapotranspirasie van die wingerdstokke op die twee verskillende gronde by elk van die twee lokaliteite, wat verskil in mesoklimaat en topografie, te bepaal en vergelyk. Die atmosferiese toestande by die twee lokaliteite gedurende die 2002/03 seisoen is ook bepaal en vergelyk met die langtermyn gemiddelde atmosferiese toestande. Die vlakke van waterstres in wingerdstokke op elke grond by elke lokaliteit is ook gemeet. Gedurende die 2002/03 groeiseisoen, was die atmosferiese toestande relatief warm en droog in vergelyking met die langtermyn gemiddeldes van vorige seisoene. Hierdie kondisies aksentueer die effek van sekere grondeienskappe wat nie noodwendig na vore kom gedurende normale, natter seisoene nie. Die gewoonlike nat Tukulu grond by Helshoogte was droër as verwag gedurende 2002/03 in vergelyking met die Hutton grond. As gevolg van sterker groekrag op die Tukulu grond, het wingerdstokke meer grondwater onttrek vroeg in die seisoen, wat gelei het tot ‘n lae grondwatermatrikspotensiaal en meer waterstres in die wingerdstokke. Die sterker groeikrag het meer beskaduwing van die lower asook meer waterstres veroorsaak, wat gelei het daartoe dat die dominante aroma in wyne vanaf druiwe op die Tukulu grond vars vegetatief was. Die Hutton grond het bestendig gebly in terme van opbrengs en wynkwaliteit in vergelyking met vorige seisoene. Daarteenoor het die Tukulu grond ‘n hoër opbrengs gelewer, maar met laer as gewoonlike wynkwaliteit. Die Avalon grond by Papegaaiberg het die hoogste grondwatermatrikspotensiaal behou tot die einde van die seisoen, heelwaarskynlik a.g.v. kapillêre aanvulling vanuit die ondergrond. Wingerdstokke op die Tukulu grond by Papegaaiberg het heelwat meer waterstres ondervind as op die ander drie gronde, veral later in die seisoen. Dit kan toegeskryf word aan ‘n kombinasie van faktore, die belangrikse daarvan die erge grondkompaksie vlak in die grond, wat worteldiepte en -verspreiding ernstig beperk het, wat op sy beurt nadelig is vir wingerdprestasie. Beide die grondwaterstatus en atmosferiese toestande het ‘n belangrike rol gespeel in die bepaling van die hoeveelheid waterstres wat die wingerdstok op verskillende stadiums ondervind het. Die lugtemperatuur en waterdampdruktekort was regdeur die seisoen laer by Helshoogte, die koeler terroir, as by Papegaaiberg, die warmer terroir. Gedurende blom was die Ψl laer vir wingerdstokke by Helshoogte as by Papegaaiberg, wat daarop wys dat daaglikse wingerdstok waterstatus hoofsaaklik deur die grondwaterinhoud bepaal was. Die verskil in wingerdstok waterstatus tussen die twee terroirs het geleidelik verminder totdat dit omgekeer was gedurende die na-oes periode toe Ψl in wingerdstokke by Papegaaiberg geneig het om laer te wees in vergelyking met die by Helshoogte. Die relatiewe lae voorsonop Ψl by Helshoogte het daarop gedui dat die wingerdstokke aan oormatige waterstres onderwerp was. Die wingerdstokke by Helshoogte het egter nie materiële waterstres (i.e. Ψl < -1.20 MPa) gedurende die warmste gedeelte van die dag ondervind nie, wat aandui dat gedeeltelike huidmondjiesluiting plaasgevind het om die ontwikkeling van oormatige waterstres te verhoed.Dit dui aan dat lae voorsonop Ψl waardes nie noodwendig impliseer dat wingerdstokke meer waterstres gedurende die warmste gedeelte van die dag sal ondervind nie, of visa versa. Dit sluit nie die moontlikheid uit dat negatiewe neweeffekte van gedeeltelike huidmondjiesluiting, soos ‘n vermindering in fotosintese, ‘n negatiewe effek kan hê op die wingerdstok se funksionering in die algemeen nie. Hierdie resultate stel voor dat die meting van daaglikse Ψl siklusse gedurende verskeie fenologiese stadia benodig word om die effek van terroir op die wingerdstok se waterstatus te verstaan en te kwantifiseer.

Thesis (MScAgric)--Stellenbosch University, 2012. / ENGLISH ABSTRACT: Phenolic compounds are important quality indicators of a red wine, as they can contribute to the colour of a young red wine, colour stability during ageing as well as astringency, bitterness, body and overall mouthfeel properties. Wine composition is commonly influenced by winemaking and viticultural practices. In South Africa it often happens that vines are excessively vigorous, resulting in canopies that are too dense, which in turn could have a negative effect on the quantity and quality of the grapes produced. Viticultural practices such as judicious canopy management and irrigation are designed to control vine vigour and yield, thus improving fruit ripening and colour development. Artificial shading and water deficit have been reported to have an influence on the sensory properties of red wine as well as on the flavonoid composition. These effects are dependent on a number of factors, however, including the season, cultivar, light intensity, and the extent and timing of water deficit. There is limited research on the possible interactive effects of grapevine water deficits and canopy manipulation on grape and wine flavonoid composition in Shiraz, as well as the relationships between berry and wine composition. We thus investigated the effect of canopy reduction in combination with water deficit on the phenolic and colour composition of Shiraz grapes at different levels of ripeness, and in their corresponding wines after alcoholic and malolactic fermentation as well as after six months’ ageing. This study found that it is possible to improve the phenolic composition of grapes and wine by shoot removal, and some of the tendencies in the wines were also observed after the ageing period. If the shoot removal is not performed at a very early stage, sunburn damage can occur and this will result in berries with a lower mass and volume at harvest due to excessive exposure without the berry having adapted to the imposed conditions. Harvesting at different ripeness levels also affected the chemical and phenolic composition of the grapes and resulting wines. The water deficit effect on most phenolic parameters measured in the grapes and wine was not as prominent as that of the canopy manipulation treatment. This study improved our understanding of how an improvement in the canopy microclimate of Shiraz could be reflected in the phenolic composition of wines, along with a potentially important effect of harvesting date. On this basis it may be possible to attain a specific wine style. Harvesting at a ripe stage, for example, could result in the production of wines with higher colour density and astringency, while unripe grapes could result in wines with higher levels of perceivable fresh berry attributes. In particular, canopy reduction could increase the astringency and body of wines made from grapes subjected to water deficit. / AFRIKAANSE OPSOMMING: Fenoliese verbindings is belangrike kwaliteitsparameters van rooiwyn, aangesien dit kan bydra tot die kleur van ‘n rooiwyn, kleurstabiliteit tydens veroudering sowel as frankheid, bitterigheid en mondgevoel. ‘n Wyn se fenoliese samestelling word algemeen bepaal deur wynmaak- en wingerdkundige praktyke. In Suid-Afrika gebeur dit gereeld dat wingerde uitermatig groeikragtig is, wat lei tot te digte lower wat sodoende ‘n negatiewe effek op kwantiteit en kwaliteit van die druiwe wat geproduseer word, het. Wingerdkundige praktyke soos oordeelkundige lowerbestuur en besproeiing is ontwerp om wingerdstokke se groeikrag en opbrengs te beheer, en sodoende vrugrypwording en kleurontwikkeling te verbeter. Kunsmatige beskaduwing en waterstres is gerapporteer om ‘n invloed te hê op die sensoriese eienskappe van rooiwyn sowel as op die flavonoïedsamestelling. Hierdie effekte is egter afhanklik van ‘n verskeidenheid faktore, insluitende die seisoen, kultivar, ligintensiteit en die mate en tyd van waterstres toegepas. Daar is beperkte navorsing op die moontlike interaktiewe effekte van waterstres en lowermanipulasie op die druif en wyn flavonoïedsamestelling in Shiraz, sowel as die verhoudings tussen druif en wyn samestelling. Ons het dus die effekte van lowerbestuur in kombinasie met waterstres op die fenoliese en kleursamestelling van Shiraz druiwe by verskillende rypheidsvlakke ondersoek, asook in hul ooreenstemmende wyne na alkoholieseen appelmelksuurfermentasie sowel as na ses maande veroudering. Hierdie studie het gevind dat dit moontlik is om die fenoliese samestelling van druiwe en wyn deur lootverwydering te verbeter, en sommige van die tendense is ook waargeneem in die wyn na die verouderingsperiode. Indien lootverwydering nie toegepas word by ‘n baie vroeë stadium nie, kan sonbrand voorkom en dit kan lei tot korrels met ‘n laer massa en volume by oes as gevolg van oormatige blootstelling sonder dat die korrel aangepas het by die spesifieke kondisies. Oes by verskillende rypheidsvlakke affekteer ook die chemiese en fenoliese samestelling van die druiwe en ooreenstemmende wyne. Die waterstreseffek op meeste van die fenoliese parameters gemeet in druiwe en wyn was nie so prominent soos dié van die lowermanipulasie behandeling nie. Hierdie studie het ons begrip verbeter van hoe ‘n verbetering van die lower mikroklimaat van Shiraz gereflekteer kan word op die fenoliese samestelling van die wyn, saam met ‘n potensiële belangrike effek van oesdatum. Op grond van hierdie basis is dit dus moontlik om ‘n spesifieke wynstyl te verkry. Oes by ‘n ryp stadium, byvoorbeeld, kan die produksie van wyn met ‘n hoër kleurdigtheid en frankheid tot gevolg hê, terwyl onryp druiwe wyne met hoër vlakke van waarneembare vars bessiekenmerke tot gevolg kan hê. Verlaging van lowerdigtheid kan veral die frankheid en mondgevoel van wyne gemaak van druiwe blootgestel aan waterstres, verbeter.

Dissertation (PhD)--University of Stellenbosch, 2004. / ENGLISH ABSTRACT: Understanding the complex nature of grapevine molecular biology is of great importance for viticulturists. Progress in the elucidation of key events on a genetic level could provide further insight into the underlying cues responsible for the precise control of physiological and metabolic changes during a specific condition such as fruit development. The use and analysis of molecular ‘tools’, such as promoters controlling the site and level of gene activity, could assist in the understanding of grapevine biology and serve as a platform for the future design and development of recombinant DNA protocols and strategies for Vitis vinifera L. A high-throughput gene expression system, cDNA-AFLPs, was successfully used to analyse large-scale transcriptional activity during berry ripening. Candidate cDNA fragments were selected on the basis of desired expression patterns and/or known gene function for subsequent promoter isolation. From three candidate cDNAs selected, the promoter of a gene encoding vacuolar pyrophosphatase (V-PPase) was isolated for computational and comparative analyses. Promoter activity was evaluated on a transient level using the green fluorescent protein (GFP) reporter gene. Comparative integration has allowed for putative correlation of cis-elements, acting as receptors within promoter regions, to regulate V-PPase gene expression in response to development, environmental stress and tissue-specificity. In this study, integration of genetic data have advanced the understanding and transcriptional role of a key enzyme (V-PPase) during grape ripening. Although never a replacement for experimental verification, this integrative strategy of combining gene expression profiles with bioinformatics and regulatory data will greatly assist in further elucidation of various other key components and regulatory cues associated with grapevine molecular biology. This study has allowed us to use molecular tools that could assist in gaining further insight into genetic complexities and could serve as a platform for a more refined genetic manipulation strategy in Vitis vinifera L. / AFRIKAANSE OPSOMMING: Begrip van die komplekse aard van wingerd molekulêre biologie is van groot belang vir wingerdkundiges. Vooruitgang in die begrip van belangrike gebeurtenisse op ń genetiese vlak behoort verdere insig in die onderliggende instruksies vir die noukeurige beheer van fisiologiese en metaboliese veranderinge tydens ń spesifieke kondisie soos vrug rypwording te bevorder. Die gebruik en analise van molekulêre ‘instrumente’ soos promoters, wat die posisie en vlak van geen aktiwiteit beheer, kan bydra tot n beter begrip van wingerd biologie en sodoende dien as ń platform vir die toekomstige ontwerp en ontwikkeling van rekombinante DNS (deoksiribonukleiensuur) protokolle en strategieë vir Vitis vinifera L. ń Hoë-kapasiteit geen uitdrukkings sisteem, nl. kDNS-AFLPs (komplementêre deoksiribonukleiensuur- geamplifiseerde fragment lengte polimorfisme), is suksesvol gebruik vir die analise van grootskaalse transkripsionele aktiwiteit tydens druif rypwording. Kandidaat kDNS fragmente is geselekteer, gebaseer op verlangde uitdrukkings-patrone en/of bekende geen funksie vir daaropvolgende promoter isolering. Van drie geselekteerde kandidaat kDNS fragmente, is die promoter van ń geen wat vakuolêre pirofosfatase (V-PPase) kodeer geïsoleer vir rekenaar- en vergelykende analise. Promoter aktiwiteit is op ń nie-stabiele vlak deur die gebruik van ń groen-fluoresserende proteien (GFP) verklikker geen geëvalueer. Vergelykende integrering het dit moontlik gemaak om veronderstelde korrelasies van cis-elemente, wat as reseptore binne ń promoter area dien, en die regulering van V-PPase geen uitdrukking, in reaksie tot ontwikkeling, omgewings stres en weefsel-spesifisiteit, te maak. Tydens hierdie studie, het die integrering van genetiese data gehelp om die transkripsionele rol van ń belangrike ensiem (V-PPase) tydens druif rypwording beter te verstaan. Alhoewel dit nooit ń plaasvervanger vir eksperimentele bewyse sal wees nie, kan hierdie gëintegreerde strategie, wat die kombinasie van geen-uitdrukkingsprofiele met bioinformatika en regulatoriese data behels, grootliks bydra om verskeie ander belangrike komponente en regulatorieseaanwysings geassosieërd met wingerd molekulêre biologie te ontrafel. Hierdie studie het verdere insig in genetiese kompleksiteite verleen, en kan nou dien as ń platform vir ń meer presiese genetiese manipulering strategie in Vitis vinifera L.

Call number: LD2668 .T4 1956 G46 / Master of Science

Thesis (MScAgric)--Stellenbosch University, 1954. / No Abstract Available

A two-year fertilizer study on a mature vineyard of Vita vinifera, C. V. Cabernet Sauvignon and Sauvignon blanc, has been conducted on White House sandy loam (fine, mixed, thermic Ustollic Haplargid), at Page Ranch, International Agricultural Center. Furthermore, eleven different varieties grown at Page Ranch and treated with different levels of nitrogen fertilizer were tested for total P content in plant tissue and NO₃-N. Moreover, in another site with similar soil, a Cabernet Sauvignon grown at the Vina Sonoita Vineyard was treated with both soil and foliar applications of P fertilizer, and was tested for total P content in plant tissue. Soil phosphorus (P) fractions of the White House soil were determined in order to evaluate the various forms of soil P and determine the most important form which contributes most to the availability index. The objectives of this study were to study the various inorganic P forms of White House soil, compare two methods of extracting available P (Olsen and Bray P.), and examine the relationships between soil pH, extractable Al, Fe, Mn, and Zn with the availability index of soil P. Furthermore, the effect of P rates and placement on the P status of plant tissue, grape yield, wine quality and petiole-P to blade-P ratio had been investigated. Three different placements of surface, 25 and 50 cm depth at rates of 88 g of P and 80 g of N per vine, were in RCB design in 1987. In 1988, three P rates of 0, 88, and 176 g of P and 207.5 g N per vine, and the two mentioned varieties CS and SB were arranged in RCB design with factorial type. The results showed that placements did not have a significant effect of P status in plant tissue and grape yield. Yet grapes showed a significant response to P fertilizer in terms of increasing P content in plant tissues and grape yield. Petiole P to Blade P ratios were calculated for the CS, and SB grown at Page Ranch, as well as for CS grown at Sonoita vineyard. This ratio is a good indicator for diagnosis of P status in a given vineyard. Nitrogen stress had its clear and profound effect on P content of grape petioles.

Effects of trellising on water use, absorption of photosynthetically active radiation (PAR, 400 to 700 nm wavelength) by foliage and potentially by fruit, fruit composition, and yield were studied in 1988 under semi-arid conditions on field-grown Vitis vinifera L. 'Petite Sirah' grapevines in a mature vineyard. The positioning of shoots on trellises resulted in canopies that were (1) positioned vertically (vertical canopy), (2) positioned in a "V" pattern with sides inclined 60° from horizontal (inclined canopy), and (3) inclined toward the vineyard floor (standard canopy). Seasonal water use values determined from neutron scattering data were 393 ± 61, 554 ± 73, and 455 ± 57 mm for the standard, vertical and inclined canopies, respectively. On average, about 50% of seasonal water consumption occurred between fruit set and filling stages for each type of canopy. Average crop factors (ratio of actual to reference crop evapotranspiration) of 0.383, 0.540 and 0.444 were for the period bud burst to harvest for standard, vertical and inclined canopy systems, respectively. The diurnal water use patterns of the three trellising canopies were very similar when measured by either the heat pulse technique or by porometer. The average daily heat pulse velocity (HPV) for selected vertical, inclined and standard canopies for 6 days were 8.77, 7.58 and 6.85 cm h⁻¹, respectively. The HPV technique indicated that the average daily water use of the whole plant was 0.227, 0.192 and 0.137 kg/m² leaf area/d for standard, vertical and inclined canopies, respectively. The daily average transpiration rates as measured by the HPV technique were 32, 31, and 25% higher than the average transpiration rates estimated from porometer data for standard, vertical and inclined canopies, respectively. Stomatal conductances of the vertical and inclined canopies were 20 and 40%, respectively, below that of standard canopy. PAR absorption by foliage during mid-day was highest in the standard trellis, and lowest in the inclined trellis. PAR potentially available for absorption by fruits was lowest in the standard trellis, and highest in the inclined trellis. In both inclined and vertical canopies, the average sunfleck values were 26%, but was only 2% in the standard canopy. Analysis of fruit composition at harvest revealed that total dissolved solids (°Brix) was significantly higher in the inclined trellis than for the vertical trellis or the standard trellis. The inclined trellis resulted in the highest alcohol content of wine. Per vine yields did not differ significantly among the three trellis systems. Overall, the standard trellis was optimum because grapevines consumed less water and produced a shading which protected the fruit from direct solar radiation.

Dinitro-ortho-cresol (500, 1000, and 2000 ppm) and dormant oil (2.5, 5.0, and 10%) were applied to Thompson Seedless and Perlette grapevines alone and in combination to enhance budbreak and fruitfulness. Applications were made immediately after pruning. Use of these materials during the winter, immediately after pruning, had no effect on either fruitfulness or budbreak in Perlette or Thompson Seedless. Thompson Seedless cuttings collected in August were exposed to six temperatures (7, 13, 18, 24, 29 and 35 C) for five time periods (2, 4, 6, 8 and 12 weeks). After temperature treatment the cuttings were planted in the greenhouse at 24 (+OR-) 1 C. Cuttings exposed to 24, 29 and 35 C broke sooner than those exposed to 7, 13, 18 C at all time periods. The percentage of the bud openings of cuttings at 29 C for a period of 2 and 4 weeks was 95% and 100% respectively. The optimum temperature for budburst was 29 C. Gibberellic acid, Thiourea, dinitro-ortho-sec-butyl-phenol (DINOSEB), potassium nitrate and Endothal were used at two concentrations alone and in combination, to break rest of Thompson Seedless buds. Thiourea at 2% and DINOSEB at 1000 ppm alone were the only treatments which gave a higher percentage of bud opening after 20 forcing days at 25 (+OR-) 1 C temperature. Similar results were obtained from cuttings taken in both winter and summer. Three times after harvest, Perlette and Thompson Seedless were defoliated using the senesce enhancer Endothal. Defoliation times (4, 8, and 12 weeks) after harvest were used. In half of the treatments, regrowth was controlled with Endothal. Gibberellic acid (1000 ppm) Thiourea (2%) and DINOSEB (2000 ppm) were applied at the time of defoliation. The four- and twelve-week defoliation periods with new vine growth controlled for improving budbreak and fruitfulness were best. Growth regulators did not improve the defoliation treatments. The best treatments hastened budbreak by 10 days, more than doubled vine fruitfulness, and increased sugar content in berries resulting in a 10-day earlier harvest than in the control. Results were similar in both Thompson Seedless and Perlette. Thompson Seedless and Cardinal vines grown under Arizona desert conditions were defoliated 4 weeks after harvest with 2000 ppm Endothal. Two weeks later they were pruned and treated with 1000 ppm gibberellic acid, 2% Thiourea, and 1000 ppm DINOSEB. Cardinal and Thompson Seedless vines produced a second commercial crop in December of the same year. Thiourea (2%) and 1000 ppm DINOSEB did not have a significant effect; however, 1000 ppm Gibberellic acid reduced the number of clusters per vine.

Ten winegrape vineyards consisting of different cultivars were leaf petiole sampled at bloomtime. Petioles were analyzed and results composited for the survey. There were indications that boron, iron, nitrogen and phosphorus were nutrients where potential problems (deficiencies) were likely to occur. This survey supplied information for the basis of developing a monitoring program on an annual basis.