The Late Hemphillian (latest Miocene or earliest Pliocene, 7-4.5 Ma) Gray Fossil Site in northeastern Tennessee is interpreted to represent a lacustrine paleokarst fed by a river or stream. This research focuses on the morphological and systematic relations of Nyssa endocarps (fruit pits) from the fossil site to extinct and extant Nyssa species. A combination of metric and nonmetric traits allows recognition of a new species: Nyssa grayensis sp. nov. This fossil species shares the most similarities with the extant Nyssa ogeche Bartram ex Marshall from southeast North America and the Eocene fossil Nyssa eolignitica Berry from western Tennessee. Affinities with Nyssa ogeche Bartram ex Marshall suggest a warmer winter climate than the present and periodically fluctuating water levels. Fossil vertebrates (Alligator, Heloderma, Hesperotestudo) and plants (Fossil relatives of Ilex vomitoria, Quercus virginiana, Taxodium, Acer leucoderme) with modern counterparts distributed in areas with mild winters support this interpretation.

Discovered in 2000, the Gray Fossil Site provides a snapshot of the flora and fauna that lived during late Miocene to early Pliocene time in eastern Tennessee. These fossils occur in sediments consisting of fine-grained clays and sands of lacustrine origin, which were deposited after multiple sinkholes formed in the underlying Knox Group basement carbonates. Three-dimensional nearest neighbor analysis has been applied to fossils of Tapirus polkensis, characterizing the spatial patterns exhibited. These analyses determined the importance of taphonomic and depositional processes that occurred during the sites formation. Six characteristics were analyzed, four at the bone level including carnivore utilization, weathering, abrasion, and arthritis, and two at the specimen level, articulation and age class. Weathering, arthritis, and articulation, show clustered patterns indicating that the site had active predators, it consisted of many microenvironments, and deposition occurred in a passive setting. Although the current state of excavation makes any spatial analyses and taphonomic interpretations difficult, spatial analysis in both dimensions can be accomplished.

Trachemys (Testudines: Emydidae) represents one of the most well-known turtles today. The evolution of Trachemys, while being heavily documented with various fossil representatives, is not well understood. Numerous fossils from the Gray Fossil Site in northeastern Tennessee are helping to elucidate the taxon’s evolution. The fossil Trachemys at the Gray Fossil Site represent a new species. A review of previously named Trachemys taxa reveals that a huge majority of them are not valid or have been previously misinterpreted. A phylogenetic analysis preformed on modern emydids and supposed fossil representatives of those genera also shows the evolution of a portion of the modern turtle fauna. While some fossils are nested within modern clades, a large number of the fossil turtles studied fall within their own clade. This may mean that these fossils represent an extinct lineage and less fossil representatives of modern turtles are known than previously thought.

The Mio-Pliocene age Gray Fossil Site of northeastern Tennessee has the largest collection of tapir postcranial skeletons in the world. Though representing a single species, a few localized structures show high variability. This paper deals with variation of the first metatarsal, which in tapirs was reduced as an early adaptation for running and then retrofitted to serve as a special origin for flexors and adductors of the proximal phalanges. The first metatarsal connects the medial ankle with a posterior process of the third metatarsal in tapiroids. In Tapirus indicus, T. webbi, and 6 out of 31 T. polkensis feet at Gray, it extends more laterally to articulate with the fourth metatarsal. This condition is too variable for species distinction but is correlated with a decrease in the metatarsophalangeal joint facet, suggesting a mobility reduction likely related to the increased range and feeding strategy seen in extant T. indicus.

Individuals of Tapirus polkensis from the Gray Fossil Site exhibit an excellent level of preservation. Intact skulls collected from the site were arranged in a rough age class system separated into 7 categories based on the teeth present and amount of dental wear. Such an eruption series is useful for general comparisons amongst the individual tapirs; however, the classes do not represent an age in years due to a lack of data on living tapirs. Consequently, it is possible that some age classes may contain several years of a tapirs life, or comparatively only a few months. In this study I placed ages on individuals of T. polkensis from the Gray Fossil Site based on age data taken from The Baird's Tapir Project of Costa Rica (Baird's Tapirs, T. bairdii) ranging from several months to 7 years in age. As eruption data is only useful for aging tapirs up to 7 years, this study also took dental wear into account for adult tapirs. After aging all possible individuals in the sample, the sample was compared to other studies on perissodactyl population structure. Interestingly, the T. polkensis sample is remarkably similar to modern populations of Diceros bicornis.

The analysis of samples recovered from the abdominal area of an articulated tapir from the Late Miocene (4.5-7 million bp) Gray Fossil Site, revealed a rich palyno-fauna comprised of about 94% egg/oocyst-like structures and 6% pollen and other palynomorphs. In addition, a tight grouping of six hickory nuts (Carya) was recovered from the same area suggesting that the samples represent the abdominal contents. The analysis of a sample from immediately outside the tapir produced a sample with 98% pollen and less than 0.5% egg-like structures. The size, shape, and general morphology of egg-like structures were analyzed with light and scanning electron microscopy and were compared to a variety of intestinal eggs found in extant ungulates, and in particular the Perissodactyla. We also compared the fossil structures to the numbers and kind of intestinal parasites recovered from fecal samples from the Baird's tapir (Tapirus bairdii) in Costa Rica and from samples collected from the Lowland tapir (T. terrestris) from Ecuador to assess their similarity to our fossil sample. Based on these data we discuss what role parasites may have played in the biology of T. polkensis during the late Miocene-early Pliocene.

Since the early days of the discovery of the Gray Fossil Site (GFS), meticulous efforts to preserve the spatial data were a priority. Direct surveying of fossils prior to recovery, as well as grid mapping the site, provided relative spatial data within a square meter. Such efforts meant that even fragments and microfossils, recovered during the screening operations and eventual concentrate picking, maintained some spatial data. Available spatial data are used to identify smaller deposits within the greater system, as well as non-random distribution patterns among a number of GFS taxa. Patterns are particularly pronounced in the large-bodied taxa, Teleoceras aepysoma and the GFS mammutid, as well as microvertebrates. Results suggest that controlling factors include taphonomic constraints related to body size, proximity to near-shore or shallow areas, underlying geological features, and to a lesser extent “ecological role”. Results of this research may help guide future excavation and collection methods.

Two peccary species, Mylohyus elmorei and Prosthennops serus are described from the medium-bodied fauna of the Gray Fossil Site (GFS) of northeastern Tennessee. This site, recognized as an oak-hickory forest, is latest Hemphillian or earliest Blancan based on mammalian biochronology, with an estimated age of 4.9-4.5 Ma. The GFS represents the only site outside the Palmetto Fauna of Florida with M. elmorei, greatly expanding the species range north over 920 km, well into the Appalachian region. This is also the first Appalachian occurrence of the relatively widespread P. serus. Our understanding of intraspecific variation for both M. elmorei and P. serus is expanded due to morphological and proportional differences found in cranial and dental material from the GFS, Tyner Farm locality, Palmetto Fauna, and within the literature. The GFS M. elmorei material represents the most complete mandible and second cranium for the species, and preserve intraspecific variation in the length of the diastema, dental proportions, and the complexity of the cuspules of the hypoconulid complex. Similarly, mandibular material from the GFS for P. serus exhibited larger dentitions and a greater degree of robustness than currently recognized for the species.

The Gray Fossil Site, northeastern Tennessee, is formed by multiple karst sub-basins filled with lacustrine sediments. The oldest sediments found were recently dated as Paleo-Eocene by palynological means, whereas the youngest sediments are considered Mio-Pliocene based on their faunal assemblage. In this study, we examined the Mio-Pliocene lacustrine sediments from the Gray Fossil Site to determine the Late Neogene floral characteristics of a site within the southern Appalachian Mountains. The Mio-Pliocene lacustrine sinkhole fill preserves a unique fossil assemblage, which includes invertebrate, vertebrate, and floral remains. Floral remains are represented by wood, seeds, leaves, and pollen grains. Forty-seven palynological samples from six different test-pits were analyzed. All pits exhibit a low pollen yield, a result of basic pH levels, drought, and fire events that occurred during deposition. The palynofloral assemblage has a low to moderate diversity and is largely dominated by a Quercus-Carya-Pinus assemblage (~ 90% of the palynoflora). The presence of Pterocarya grains supports a Late Neogene age for these lacustrine sediments. Comparison with modern pollen-based floras from North America suggests that: (1) examined pits can be discriminated into two separate groups based on their palynofloral signatures, (2) the Mio-Pliocene vegetation at the site ranged between a closed to open woodland setting, depending on the intensity and frequency of drought and fire events, and (3) the fossil palynofloral assemblage is comparable to what would be expected in the modern North American Mesophytic Forest region.

In order to understand Mio-Pliocene floristic characteristics of the southern Appalachian Mountains, 47 palynological samples from six different testing-pits across the Gray Fossil Site (GFS) were analyzed. The site exhibits a low pollen yield resulting from basic pH levels, drought, and fire events occurring during deposition. The palynofloral assemblage has a low to moderate diversity, and it is largely dominated by Quercus-Carya-Pinus (~90% of the palynoflora). The reported presence of Pterocarya grains supports a Late Neogene age for the lacustrine sediments. Comparison with modern pollen-based floras suggests that: (1) the Mio-Pliocene Oak-Hickory-Pine association varied in structure between a woodland to woodland/savanna, depending on the intensity and frequency of drought, fire events and herbivory, (2) pits show different structure of the co-dominant genera, which indicate alternating composition of the vegetation, and (3) in term of modern vegetation, the GFS flora corresponds well with the Mesophytic Forest region.