Pentachlorophenol (PCP) is a priority environmental pollutant in North America that was widely used as a wide-spectrum biocide in the last century. Pentachlorophenol 4-monooxygenase (PcpB) is the first and rate-limiting enzyme in the PCP biodegradation pathway in <i>Sphingobium chlorophenolicum</i> ATCC 39723.<p> Pentachlorophenol 4-monooxygenase (PcpB) catalyzes the hydroxylation of pentachlorophenol in the pentachlorophenol biodegradation pathway in Sphingobium chlorophenolicum. Previous studies from two different research groups proposed oppositely that the catalytic product of PcpB was tetrachlorohydroquinone (TCHQ) and tetrachlorobenzoquinone (TCBQ). We re-examined the identity of the catalytic product of PcpB, because TCHQ and TCBQ are present in a redox-equilibrium in aqueous solutions and the chemical reagents NADPH, ethyl acetate and glutathione used for the product detection in the previous studies may shift the redox-equilibrium. In this study, we investigated the effects of NADPH, ethyl acetate and glutathione on the redox-equilibrium and product distribution. Under newly designed experimental conditions, we confirmed unambiguously that the catalytic product of PcpB is TCHQ instead of TCBQ. We also propose that TCBQ may be produced non-specifically by peroxidases within the bacterial cells and that TCBQ reductase (PcpD) might act as a self-protective rather than a PCP-degradation enzyme. <p> There is no glutathione S-transferase (GST) activity for wild type of PcpC, PcpC (C13S) mutant and a surface loop deletion mutant PcpC (LD). In the crystallization studies, PcpC (C13S), PcpB and PcpA were over-expressed and purified to >95% purity using Ni-NTA based affinity chromatography. Initial crystallization conditions have been obtained for all three enzymes from both in-house screening using the screening kits from Qiagen-Nextal and high throughput screening at the Hauptman-Woodward Institute, Buffalo, New York. Further structural determination studies will be undertaken upon obtaining large crystals suitable for X-ray diffraction data collection.

The aim of this master thesis was the finding out of potential of growing the soybeans in the area with not so suitable growing conditions. The next aim was to perform the experiment with application of leaf fertilizer EGT Fulhum which increases root system volume. In the years 2016 and 2017 was in the altitude 395 m established the field experiment with the variety Amandine and since the year 2017 was also joint the experiment with the very early variety Abeline. Within these cultivars of soybean were evaluated these parameters: Seed yield, the 1000 seed weight, seed oiliness, plant height, number of pods on one plant, number of the early branches and weight of roots. The seed yield was very variable from the point of view of the year. The yield in the year 2016 was above-average, the control variant reached the yield 2,93 tons per hectare and variant treated by Fulhum 2, 78 tons per hectare. One of the studied parameter was the nitrogen content and based on the results of this parameter was noticeable reduction of nitrogen content of the treated variant in the comparison with control variant. In the further year were two compared two varieties - Amandine and Abeline. Within the both varieties were involved to the experiment the control variant and the variant treated by plant auxiliary substance. In the year 2017 was confirmed the influence of the crop area establishment on the yield quality parameters. The progress of weather confirmed the high requirements of soybean on the good rainfall conditions. Seed yield of the variety Amandine was 1,07 tons per hectare in the case of the treated variant, by control variant it was 0,88 tons per hectare. The difference of the seed yield between both variants of the variety Abeline was very little, it was 0,90 tons per hectare by the treated variant and 0,93 tons per hectare by the control variant. The application of the plant auxiliary substance didn't cause, on average, the improvement of the studied parameters so the using of this substance, for the improvement of these parameters almost doesn't make any sense.

Syfte: Syftet med denna litteraturstudie var att undersöka hur forskning har beskrivit uppväxten för barn som lever med en mamma med intellektuell funktionsnedsättning och vad det finns för risk- och skyddsfaktorer kring barnen. Samt om eller på vilket sätt föräldraförmågan påverkar barnens uppväxtförhållanden. Metod: En kvalitativ litteraturstudie bestående av 14 artiklar och en bok. Databassökningarna utfördes via databaserna SOCIndex, SWEpub och Discovery. Dataanalysen utgjordes av en tematisk analys. Huvudresultat: Litteraturstudien har identifierat tuffa uppväxtförhållanden hos en betydande majoritet bland barn som lever med en mamma med intellektuell funktionsnedsättning. Resultatet tyder på att målgruppen bör betraktas som en riskgrupp. Denna målgrupp har visats ha sämre levnadsvillkor på grund av mammans intellektuella funktionsnedsättning. / Aim: The aim of this literature study was to investigate how research has described the childhood of children living with a single mother with intellectual disability and what the risk and protection factors are around the children. And whether or in what way parenting ability affects the children's childhood conditions. Method: A qualitative literature study consisting of 14 articles and a book. The database searches were performed through the databases SOCIndex, SWEpub and Discovery. The data analysis consisted of a thematic analysis. Main results: The literature study identified difficult upbringing conditions in a significant majority of children living with a mother with intellectual disability. The results indicate that the target group should be considered a risk group. This target group has been shown to have poorer living conditions due to their mother's intellectual disability.

Conselho Nacional de Desenvolvimento Científico e Tecnológico / The objective of this study was to evaluate the processing quality of potato tubers to identify superior clones under different growing conditions of Southern Brazil. Two trials were carried out in different conditions. The first trial was carried out during summer in São Joaquim, SC, and during spring and fall seasons in Júlio de Castilhos, RS to evaluate the genotype x environmental interaction for tuber quality and the gain from selection. A total of 18 potato genotypes were evaluated for chip color, dry mass, reducing sugar and amylose contents. Summer growing conditions increase the gain from selection and the selection is correlated with both spring and autumn seasons. The second trial was carried out to evaluate the yield and the tuber quality of potato clones in early harvest during autumn and spring seasons in Júlio de Castilhos, RS. A total of 12 potato genotypes were evaluated for yield, dry mass, sucrose and reducing sugar contents in tubers harvested at 70 and 90 days after planting (DAP), autumn season, and 70 DAP, spring season. Potato tubers harvested at 90 DAP in autumn and at 70 DAP in spring do not get the minimal processing quality necessary for the industry. / O objetivo desse trabalho foi avaliar a qualidade de processamento de tubérculos de batata para a seleção de clones em diferentes condições de cultivo no Sul do Brasil. Para isso foram considerados dois estudos. No primeiro estudo, foram conduzidos experimentos em condições de cultivo de verão em São Joaquim (SC), e de primavera e outono, em Júlio de Castilhos (RS), representativos das principais épocas de cultivo da batata na região Sul do Brasil, com o objetivo de avaliar a interação genótipo x ambiente em caracteres de qualidade de tubérculos de batata e seus reflexos no ganho de seleção. Em cada cultivo, foram avaliados 18 clones de batata para os caracteres coloração de chips e da composição dos tubérculos em teores de matéria seca, açúcares redutores, amido e amilose. O cultivo de verão maximiza os ganhos genéticos, sendo a seleção neste ambiente correlacionada aos ambientes de primavera e outono. No segundo estudo, realizado em Júlio de Castilhos (RS), foram conduzidos experimentos com o objetivo de avaliar a produtividade e a qualidade de tubérculos de clones de batata com maturidade precoce no outono e na primavera. Foram avaliados 12 clones de batata para produtividade, matéria seca, sacarose e açúcares redutores de tubérculos colhidos aos 70 e 90 dias após o plantio (DAP) no outono e aos 70 DAP na primavera. Os tubérculos colhidos aos 90 dias após o plantio de outono e aos 70 dias após o plantio de primavera ainda não atingiram a qualidade mínima para o processamento industrial.