No description available.

No description available.

Throughout the years lime-induced chlorosis has contributed to untold economic losses. Although this disease has challenged the technical ingenuity of outstanding plant and soil scientists, the exact cause has never been determined; consequently, no preventative measures or permanent cures can, as yet, be recommended. This physiological malady, unchecked, continues its rampage in regions where the disease is prevalent.

Rhodococcus has diverse metabolic capabilities, such as delaying ripening of certain climacteric fruit. Nitrile hydratase (NHase), amidase, 1-aminocyclopropane-1-carboxylate deaminase (ACC deaminase), cyanidase, and β-cyanoalanine synthase-like enzyme (βCAS-like) are possibly involved in fruit ripening. The activity of these enzymes in Rhodococcus rhodochrous DAP 96253 cells were induced with selected multiple inducers (i.e. cobalt and urea). This research showed that the supplementation of selected sugars, i.e. trehalose and maltodextrin in growth media and storage buffers of R. rhodochrous DAP 96253 affected activity and stability of the enzymes mentioned above. Thermostability and osmostability of the five enzymes in whole cells (plate grown and fermented) were evaluated in this study, i.e. βCAS-like was more stable than the other four enzymes in storage conditions. Immobilized biocatalysts have practical advantages over the use of “free” whole cells. Immobilization of whole rhodococcal cells (plate grown and fermented) was employed, using techniques such as glutaraldehyde-polyethylenimine (GA-PEI) cross-linking, waxing and calcium-alginate entrapment. The GA-PEI immobilized catalysts were non-replicating and more stable in storage conditions than the catalysts produced by the other two methods. Wax or calcium-alginate immobilized catalysts (live catalysts) showed higher enzyme activity than the GA-PEI catalyst. The effects of whole and immobilized catalysts were evaluated on delayed ripening of fruit. Both free whole cells and immobilized catalysts delayed the ripening of bananas and peaches. Delayed ripening experiments showed that the catalysts were effective in direct contact and not in contact with fruit. Moreover, both free whole cells and immobilized catalysts showed antifungal activity against Aspergillus niger and Penicillium spp. Gas chromatography was performed to analyze volatile interactions between the biocatalysts and fruit. This analysis revealed that cyanide in an atmosphere with ethylene was utilized by the biocatalysts. There was also less volatile production by exposed fruit (bananas) than fruit unexposed to biocatalysts, either rhodococcal immobilized catalysts or live whole cells (plate grown and fermented).

Rhodococcus has diverse metabolic capabilities, such as delaying ripening of certain climacteric fruit. Nitrile hydratase (NHase), amidase, 1-aminocyclopropane-1-carboxylate deaminase (ACC deaminase), cyanidase, and β-cyanoalanine synthase-like enzyme (βCAS-like) are possibly involved in fruit ripening. The activity of these enzymes in Rhodococcus rhodochrous DAP 96253 cells were induced with selected multiple inducers (i.e. cobalt and urea). This research showed that the supplementation of selected sugars, i.e. trehalose and maltodextrin in growth media and storage buffers of R. rhodochrous DAP 96253 affected activity and stability of the enzymes mentioned above. Thermostability and osmostability of the five enzymes in whole cells (plate grown and fermented) were evaluated in this study, i.e. βCAS-like was more stable than the other four enzymes in storage conditions. Immobilized biocatalysts have practical advantages over the use of “free” whole cells. Immobilization of whole rhodococcal cells (plate grown and fermented) was employed, using techniques such as glutaraldehyde-polyethylenimine (GA-PEI) cross-linking, waxing and calcium-alginate entrapment. The GA-PEI immobilized catalysts were non-replicating and more stable in storage conditions than the catalysts produced by the other two methods. Wax or calcium-alginate immobilized catalysts (live catalysts) showed higher enzyme activity than the GA-PEI catalyst. The effects of whole and immobilized catalysts were evaluated on delayed ripening of fruit. Both free whole cells and immobilized catalysts delayed the ripening of bananas and peaches. Delayed ripening experiments showed that the catalysts were effective in direct contact and not in contact with fruit. Moreover, both free whole cells and immobilized catalysts showed antifungal activity against Aspergillus niger and Penicillium spp. Gas chromatography was performed to analyze volatile interactions between the biocatalysts and fruit. This analysis revealed that cyanide in an atmosphere with ethylene was utilized by the biocatalysts. There was also less volatile production by exposed fruit (bananas) than fruit unexposed to biocatalysts, either rhodococcal immobilized catalysts or live whole cells (plate grown and fermented).

Waste Stabilisation Ponds (WSPs) are a relatively simplistic and non-intensive wastewater treatment technology; with various WSP configurations widely employed to treat a range of different wastewaters the world over. Whilst the advantages of WSP treatment are both numerous and well recognized, performance problems relating to the presence of occasionally large and unpredictable quantities of plankton (both algal and zooplankton) biomass in the final pond effluents have posed significant operational problems for WSP operators; with this suspended biomass representing the single biggest drawback associated with the technology. Research conducted during this project was concerned with assessing a selection of so-called ‘advanced’ in-pond treatment processes for the upgrading or polishing of a final WSP effluent. The particular research emphasis was on the removal of problematic algal and zooplankton biomass from WSP effluent prior to Dissolved Air Flotation/Filtration (DAF/F) treatment and wastewater reuse at the Bolivar Wastewater Treatment Plant (WWTP) north of Adelaide. The in situ WSP upgrade systems assessed in this thesis were: the native floating plant ‘Duckweed’ (DW); ‘Rock Filters’ (RFs); and an artificial ‘Attached-Growth Media’ (AGM); all of which were assessed for their relative treatment efficacies parallel to a non-interventional ‘Open Pond’ (OP) system which served as an effective control. These performance comparisons were assessed on a pilot-scale using a custom made pilot treatment plant which was located at the Bolivar WWTP. Performance monitoring was periodically carried out over a 12 month period from July 2005–August 2006, with algal and zooplankton populations monitored in addition to the more conventional wastewater quality parameters. Results from pilot plant investigations demonstrated that of the four pilot upgrade series, the RF and AGM systems displayed the greatest treatment potential in terms of both the magnitude and reliability of suspended solids, algal and zooplankton biomass removals. The DW system was also shown to be at least as effective and in some instances significantly more advanced than the uncovered OP system in terms of its ability to significantly improve the final effluent quality of the Bolivar WSPs. Both the RF and AGM upgrades (and to a lesser degree also the DW system) were found to offer considerable potential for producing a higher quality WSP effluent for more efficient processing by the Bolivar DAF/F plant; although there were various operational advantages and disadvantages as well as varying capital establishment costs associated with each of the candidate technologies. This part of the research represented the first direct performance comparison between two popular pond upgrade technologies (i.e. RFs and DW) and also constituted the first assessment of a novel AGM for the upgrading of tertiary-level WSP effluent. In addition to this, results from ecological performance monitoring also provided the first detailed insights into algal and zooplankton population dynamics within these WSP upgrade environments. In addition to these pilot-scale WSP upgrade performance investigations, another branch of the research project investigated additional research questions regarding the survival of algal cells within these pond upgrade environments. A series of laboratory experiments attempted to recreate the in situ conditions (in terms of light and oxygen availability) that might exist within the adopted upgrade environments. Using two common WSP algal species, long-term monitoring of the physiological status of phytoplankton cells during prolonged dark-exposure under conditions of reduced oxygen availability was performed in order to assess the likely effects of these particular environmental conditions on their survival potential in situ. Results from these laboratory-based experiments showed that both algal species were capable of quickly adjusting their cellular metabolism in response to dark incubation. Results also showed that a reduced environmental oxygen concentration (25% of saturation) had no bearing on the ability of either Chlorella or Chlamydomonas species to withstand long-term dark-exposure; with both species retaining what was essentially full biological viability following up to two months of continuous dark-exposure. In an applied context, these results suggested that subjecting algal cells to conditions of simultaneous darkness and reduced oxygen availability would be expected to have no significant adverse effects on algal survivorship within an advanced in-pond upgrade system such as a duckweed-covered WSP, a rock filter or an AGM system.

Rhodococcus is an important industrial organism that possesses diverse metabolic capabilities, it also has a unique cell envelope, composed of an outer layer of mycolic acids and glycolipids (free or bound lipids generally linked to the sugar trehalose). Rhodococcus is able to transform nitriles to the corresponding amide by the enzyme Nitrile Hydratase (NHase), therefore rhodococcal cells can be utilized as biocatalysts in the detoxification of nitrile waste water or in the production of industrially important amides such as acrylamide. However, the NHase within the native cells must be stable with high activity. This research examined how NHase activity and stability can be increased in native cells by changing growth media composition, the impact on the rhodococcal cell envelope was also studied. Growth media composition was altered by supplementing different sugars such as fructose, maltose or maltodextrin to replace glucose in rich solid media containing cobalt and urea for induction of NHase. The supplementation of maltose or maltodextrin resulted in significantly higher NHase activities and greater NHase stability at 55„aC. The supplementation of these different sugars was shown to alter cellular and lipid bound trehalose levels, a sugar known to stabilize proteins and a component of the rhodococcal cell envelope. Cells that had higher levels of cellular trehalose had significantly greater NHase stability at 55„aC. The effect of the different sugar supplements and inducers of NHase, such as cobalt, on cell envelope components such as mycolic acids and glycolipids were examined by High Performance Liquid Chromatography (HPLC) and Thin Layer Chromatography (TLC). The results showed that changes in mycolic acids and glycolipids occurred when the cells were grown in the presence of different sugar supplements and when the cells were induced for NHase. Susceptibility of Rhodococcus sp DAP 96253 to different antibiotics was examined to indicate if changes were occurring in the cell envelope. Differences in antibiotic susceptibility were observed when the cells were grown on media with different sugar supplements and when the cells were induced for NHase. In the presence of cobalt Rhodococcus sp DAP 96253 showed a significant increase in sensitivity to antibiotics. Changes in growth media composition influences the cell envelope of Rhodococcus sp DAP 96253 and also affects NHase activity and stability. Therefore, achieving increased enzyme activity and stability is not entirely dependent on the actual enzyme, but is related to other aspects of the cell, such as the cell envelope and metabolites of the cell.

MSCAGR ( Plant Production) / Many high valued tree species of medicinal significance in the Limpopo Province, South Africa exhibit seed dormancy, and also contain aromatic oils which inhibit rooting of their stem cuttings. These plant species are under pressure due to human over-exploitation. The main objective of this study was to investigate effects of rooting hormones on the rooting ability that will help in domesticating some of the selected high valued medicinal plants, Elaeodendron transvaalense (bushveld saffron), Brackenridgea zanguebarica (yellow peeling plane), and Warburgia salutaris (pepper-bark tree). Stem cuttings of these plant species were prepared and treated with various concentrations (500, 1000 and 2000 ppm) of IBA, IAA and NAA in different growth media (Natural soil, farm soil and hygromix) at a nursery house. 180 experimental units were sown and arranged in Randomized Complete Block Design (RCBD), each treatment replicated five times and then monitored for a period of three months. Data were only obtained from Brackenridgea zanguebarica as other species dried up two weeks after sprouting. The two variables measured from the experiments were sprouted stems and number of leaves. The data obtained were subjected to analysis of variance and least significant difference (LSD) at 5% probability level was used to compare treatment using STATISTICA software analysis package. The hormone, hormone concentration, growth media and their interactions had effect on sprouted stems and number of leaves produced on Brackenridgea zanguebarica cuttings, with no record of rooting ability. IBA (500 ppm and 1000 ppm) and control (without rooting hormone) showed high significant results with natural soil and farm soil in terms of leaf production compared to the hygromix, which is significantly lower from others. IBA at the various concentrations (500, 1000 and 2000 ppm) and the control gave the highest percentage sprouted stem on both natural soil and farm soil as compared with other hormones at the same / NRF

Peat extraction for horticultural production poses a threat to wetland ecosystems. The rapid growth rate of the horticulture industry has prompted an ongoing search for sustainable alternative growth media components to replace peat. The alternative components need to provide properties (physical and chemical) similar to or better than peat and provide conditions that will enhance ideal growth and yield of potted plants. Potted Dendranthema x grandiflorum is one of the most important pot plants cultivated worldwide in the floriculture industry. There is a global research effort to replace peat with a sustainable alternative growth media for potted plants; however, so far, no study has been conducted in South Africa that used similar treatments on potted Dendranthema x grandiflorum. The aim of this study was to determine a suitable alternative growth media to replace peat as a growth media for cultivation of potted D. x grandiflorum. A greenhouse experiment was conducted at the University of South Africa’s Horticulture centre in Florida, Johannesburg for 89 days. Eight growth media (100 % peat (T1) (control), 100 % bagasse (T2), 50:50 % v/v bagasse:peat (T3), 75:25 % v/v bagasse:peat (T4), 25:75 % v/v bagasse:peat (T5), composted bagasse (T6), Coir (T7), and pine bark (T8)) as treatments and one hybrid (Mount® Runca) of D. x grandiflorum were arranged in a randomized complete block design with four replicates. In this study, nutrient uptake, chlorophyll content, growth, and yield parameters were measured for potted D. x grandiflorum grown in all eight growth media. The results show that treatments had different chemical and physical properties compared to peat. The pH of 100 % bagasse and coir were within the ideal range recommended for growth media. The EC results indicated that after the experiment, other treatments were within the defined range except for composted bagasse due to high concentration of soluble salts. The BD of control and composted bagasse were similar and may have resulted in the low root response. The concentration of total N was high in the shoots of plants cultivated in 100 % peat with a subsequent increased fresh and dry shoots weight. The highest significant chlorophyll content was present in plants cultivated in composted bagasse, which contained high total N and, Fe and Zn concentrations in shoots. Taken together, the results showed that composted bagasse was the best alternative to replace peat for cultivation of potted D. x grandiflorum. / Turfonttrekking vir tuinboukundige produksie hou ʼn bedreiging vir moerasland-ekostelsels in. Die vinnige groeitempo van die tuinboubedryf het gelei tot ʼn voortdurende soektog na volhoubare groeimediakomponente om turf te vervang. Die alternatiewe komponente moet (fisiese en chemiese) eienskappe kan bied wat soortgelyk aan, of beter as dié van turf is, en moet toestande gee wat die ideale groei en opbrengs van potplante verbeter. Potplant- Dendranthema x grandiflorum is een van die belangrikste potplante wêreldwyd wat in die blomboerderybedryf aangeplant word. Daar word wêreldwyd navorsing gedoen om turf met ʼn volhoubare groeimedium vir potplante te vervang; sover is daar egter nog nie in Suid-Afrika ʼn studie gedoen wat soortgelyke behandelings vir potplante- Dendranthema x grandiflorum gebruik nie. Die doel van hierdie studie was om ʼn gepaste alternatiewe groeimedium te bepaal om turf as ʼn groeimedium te vervang vir die aanplanting van D. x grandiflorum-potplante. ʼn Kweekhuis-eksperiment is by die Universiteit van Suid-Afrika se Tuinbousentrum in Florida, Johannesburg uitgevoer vir 89 dae. Agt groeimedia (100% turf (T1) (beheer), 100% bagasse (T2), 50:50% v/v bagasse:turf (T3), 75:25% v/v bagasse:turf (T4), 25:75 % v/v bagasse:turf (T5), bagasse wat tot kompos verwerk is (T6), klapperhaar (T7), en dennebas (T8)) as behandelings en een hibried (Mount® Runca) van D. x grandiflorum is in ʼn verewekansigde, volledige blokontwerp met vier repliserings gerangskik. In hierdie studie is voedingstofopname-, chlorofilinhoud-, groei- en opbrengs-parameters gemeet vir potgroei van D. x grandiflorum in al agt groeimedia. Die resultate toon dat die behandelings verskillende chemiese en fisiese eienskappe in vergelyking met turf het. Die pH van 100% bagasse en klapperhaar val binne die ideale reikwydte wat vir groeimedia aanbeveel word. Volgens die EG (elektriese geleiding)-resultate was ander behandelings binne die gedefinieerde reikwydte – behalwe vir bagasse wat tot kompos verwerk is – vanweë die hoë konsentrasie oplosbare soute. Die BD van beheer en bagasse wat tot kompos verwerk is, was soortgelyk en kon die lae wortelrespons veroorsaak het. Die konsentrasie totale N was hoog in die lote van plante wat in 100% turf aangeplant is, met ʼn gevolglike verhoging in die gewig van vars en droë lote. Die hoogste beduidende chlorofilinhoud was teenwoordig in plante wat gekweek is in bagasse wat tot kompos verwerk is, en wat hoë totale konsentrasies van N, Fe en Zn in die lote bevat het. Alles in ag genome het die resultate getoon dat bagasse wat tot kompos verwerk is, die beste alternatief is om turf te vervang in die kweking van D. x grandiflorum in potte. / Go ntsha borubu mo kumong ya mokgwa wa temo go na le matshosetsi mo matshelong a diphologolo le ditlhare tsa lefatshe le le kolobileng. Kelo ya kgodiso e e bonako ya intaseteri ya matshelo a diphologolo le ditlhare e susumetsa patlo e e tswelelang ya dikarolo tsa mekgwa ya kgodiso ya thefosano e e tswelelang ya go emela go ntsha borubu. Dikarolo tse dingwe di tlhoka go neela dipharologantsho (sebopego le khemikale) tse di tshwanang le kgotsa botoka mo go ntsheng borubu le go neela mabaka a a ka tsholetsang kgodiso e e ikaeletsweng, mme ya ntsha dijalo tse di ka fa dipitseng. Dendranthema x grandiflorum e e ka fa dipitseng ke thefosano nngwe ya dijalo tsa ka fa dipitseng tse di botlhokwa thata tse di jadilweng mo lefatsheng ka bophara mo intasetering ya mokgwa wa temo ya dithunya. Go na le boiteko jwa patlisiso ya bogotlhe ya go emela go ntsha borubu ka mokgwa wa kgodiso wa thefosano o mongwe o o tswelelang wa dijalo tsa ka fa dipitseng; le gale, go le kalo, ga go na thuto e e setseng e dirilwe mo Aforikaborwa e e dirisang ditshwaro tse di tshwanang mo go Dendranthema x grandiflorum e e mo dipitseng. Maikaelelo a thuto eno e ne e le go tlhomamisa mekgwa ya grandiflorum e e mo dipitseng. Maikaelelo a thuto eno e ne e le go tlhomamisa mekgwa ya kgodiso ya thefosano e mengwe e e tshwanelang go emela go ntsha borubu jaaka mekgwa ya kgodiso ya go jala D. x grandiflorum ka mo dipitseng. Tekelelo ya ntlo e tala e ne ya dirwa kwa lefelong la Mokgwa wa matshelo a diphologolo le ditlhare ya Yunibesiti ya Aforikaborwa kwa Florida, Johannesburg mo matsatsing a le 89. Mekgwa ya kgodiso e merobedi (100 % ya go ntsha borubu (T1) (taolo), 100 % bagasse (T2), 50:50 % v/v bagasse: go ntsha borubu (T3), 75:25 % v/v bagasse: go ntsha borubu (T4), 25:75 % v/v bagasse: go ntsha borubu (T5), bagasse e e bodisitsweng (T6), Coir (T7), le kutu ya phaene (T8)) jaaka ditshwaro le lotswakwa lo lo longwe (Mount® Runca) ya D. x grandiflorum di ne di beilwe ka moakanyetso wa boloko e e feletseng ka kakaretso ka ditshwano di le nne. Mo thutong eno, go tsaya kotlo, diteng tsa setalafatsi, kgodiso le diparametara tse di ntshitsweng di ne tsa lekanyediwa mo go D. x grandiflorum e e mo dipitseng e e jadilweng mo mekgweng ya dikgodiso tse di robedi tse tsotlhe. Dipheto di bontsha gore ditshwaro di na le dikarolo tsa dikhemikale le dibopego tse di farologaneng fa di tshwantshanngwa le go ntsha borubu. Bagasse ya pH ya 100 % le coir di ne di le magareng ga paka ya botlhokwa ya kgodiso e e atlenegisitsweng mo mekgweng ya kgodiso. Dipheto tsa EC di bontsha gore morago ga tekelelo, ditshwaro tse dingwe di ne di le magareng ga paka e e tlhalositsweng kwa ntle ga bagasse e e bodisitsweng kwa kokoanong e e kwa godimo ya matswai a a tlhaolositsweng. BD ya taolo le bagasse e e bodisitsweng, mme go ka bo go dirile gore go nne le tsibogelo ya medi e e kwa tlase. Kokoano ya N yotlhe e ne e le kwa godimo mo matlhogeding a dijalo tse di jadilweng ka go ntsha borubu jwa 100 % ka koketso e e latelang ya bokete jwa matlhogedi a mantshwa le a a omileng. Diteng tsa setalafatsi se se botlhokwa se se kwa godimodimo di ne di le teng mo dijalong tse di jadilweng ka bagasse e e bodisitsweng, e e nang le kokoano ya bogotlhe jo bo kwa godimo jwa N, Fe le Zn mo matlhogeding. Di tserwe mmogo, dipheto di bontsha gore bagasse e e bodileng jaaka sengwe se se gaisang go emela go ntsha borubu mo jalong ya D. x grandiflorum ka mo dipitseng. / School of Environmental Sciences / M. Sc. (Ornamental Horticulture)

<div> <p>A residential botanical air filtration system (Biowall) to investigate the potential for using phytoremediation to remove contaminants from indoor air was developed. A full scale and functioning prototype was installed in a residence located in West Lafayette, Indiana. The prototype was integrated into the central Heating, Ventilating, and Air Conditioning (HVAC) system of the home. This research evaluated the Biowall operation to further its potential as an energy efficient and sustainable residential air filtration system.<br></p> <p> </p> <p>The main research effort began after the Biowall was installed in the residence. A field evaluation, which involved a series of measurements and data analysis, was conducted to identify treatments to improve Biowall performance. The study was conducted for approximately one year (Spring 2017-Spring 2018). Based on the initial data set, prioritization of systems in need of improvement was identified and changes were imposed. Following a post-treatment testing period, a comparison between the initial and final performances was completed with conclusions based on this comparison. </p> <p> </p> <p>The engineering and analysis reported in this document focus on the air flow path through the Biowall, plant growth, and the irrigation system. The conclusions provide an extensive evaluation of the design, operation, and function of the Biowall subsystems under review.</p> </div> <br>