O objetivo deste trabalho foi avaliar o efeito de um preparado de anticorpos policlonais (PAP) contra bactérias ruminais específicas, Streptococcus bovis e Fusobacterium necrophorum, em parâmetros ruminais da fermentação, em vacas canuladas, adaptadas ou não a uma dieta de alta proporção de carboidratos prontamente fermentescíveis, após indução à acidose. O delineamento experimental utilizado foi o quadrado latino 3X3 replicado em arranjo fatorial de tratamentos 3X2, sendo 2 aditivos alimentares (PAP na apresentação em pó - PAPP e PAP na apresentação líquida - PAPL) mais um grupo controle (CON) e dois manejos de adaptação à dieta, resultando em seis tratamentos. O primeiro quadrado latino foi submetido a um protocolo de adaptação à dieta do tipo gradual ou step-up: dos dias D0 a D4 os animais receberam 100% de forragem; do D5 ao D9, 30% de concentrados e do D10 ao D14, 60% de concentrados. O segundo quadrado latino recebeu 100% de forragem do D0 ao D14 (sem adaptação). Nos D15 e D16, todos os animais receberam dieta com 80% de concentrados. Para as análises foram coletadas amostras de líquido ruminal a cada 3 horas a partir da 0h antes da alimentação até as 36h (D15 e D16) durante o desafio com uma dieta de 80% de concentrados. Os dados foram analisados pelo procedimento Mixed do SAS com nível de significância de 0,05. Foi observada interação entre tempo e adaptação (P<0,05) para pH ruminal com diferença entre método de adaptação nas 0, 3, 6, 9, 12 e 36 horas pós alimentação, quando o grupo não adaptado teve valores maiores que o grupo adaptado, sendo que na hora 24 ocorreu o contrário. Para a concentração de ácidos graxos de cadeia curta (AGCC), nas horas 0, 3, 6, 9 e 36 pós alimentação o grupo adaptado obteve maiores valores comparado ao grupo não adaptado. Para proporção molar de acetato, a 0 hora o grupo sem adaptação obteve valores maiores comparado ao grupo adaptado. Já nas horas 24, 27 e 30 o grupo com adaptação que obteve maiores valores. Para a proporção molar de propionato o grupo sem adaptação teve valores mais altos em comparação ao outro grupo das 3 às 36 horas pós alimentação. Quanto à proporção acetato:propionato (Ac:Pr) às 6, 12, 24, 27, 30 e 36 horas pós alimentação, o grupo de animais adaptados teve valores mais altos que o grupo não adaptado. Na proporção molar de butirato, o grupo de animais adaptados obteve maiores valores nas horas 0, 3, 6, 9, 12, 33 e 36. Para os valores de nitrogênio amoniacal (N-NH3), às 6 horas pós alimentação, o grupo não adaptado obteve maiores valores que o grupo adaptado (26,1 vs. 19,3, respectivamente). Nas horas 9, 30, 33 e 36 ocorreu o contrário. Observou-se também interação entre tempo e aditivo (P=0,0430) para a proporção molar de butirato. Porém, quando a análise foi realizada por tempo, nenhum efeito foi observado. Para os valores relativos de protozoários mensurados (Dasytricha, Isotricha, Epidinium, Diplodinium e Entodinium) apenas o Entodinium apresentou efeito de adaptação (P<0,0236) tendo sua proporção maior no grupo adaptado. Os valores de haptoglobina também não foram influenciados nem por aditivo nem por adaptação. O preparado de anticorpos policlonais não foi tão eficaz quanto a adaptação gradual à dieta de alto concentrado para controlar alterações dos parâmetros ruminais. / The objective of this trial was to evaluate the effects of polyclonal antibodies preparation (PAP) against specific rumen bacteria Streptococcus bovis and Fusobacterium necrophorum on rumen fermentation parameters in ruminally cannulated cows adapted or not to highly fermentable carbohydrates diets (HFC) after an acidosis challenge. The experimental design was two 3X3 Latin squares in a factorial arrangement of treatments 3X2 regarding two feed additives (PAP in powder presentation - PAPP and PAP in liquid presentation - PAPL) plus control group (CON) and two managements of diets adaptation, resulting in six treatments. The first Latin square had a step-up diet adaptation: from D0 to D4 100% forage; D5 to D9 30% of concentrates and D10 to D14 60% of concentrates. The second Latin square received 100% forage from D0 to D14. On D15 and D16, all animals received a diet with 80% of concentrates. For analysis, rumen fluid was sampled at 0 and every 3 h posfeeding totaling 36 h (D15 and D16) of challenge with a diet with 80% of concentrates. Data were analyzed by MIXED procedure with a significance level of 0.05. An interaction between time and adaptation (P<0,05) was observed for ruminal pH. At 0, 3, 6, 9, 12 and 36 h postfeeding, the non-adapted group had higher values compared to the adapted group and at 24 h postfeeding, the inverse was observed. For total short-chain fatty acids concentration, at 0, 3, 6, 9 and 36 h postfeeding, the adapted group had higher values compared to non-adapted group. For molar proportion of acetate at 0h postfeeding, the non-adapted group had higher values than the adapted group, and at 24, 27 and 30h, the adapted group had greater values than the non-adapted group. For molar proportion of propionate the non-adapted group had greater values compared to the adapted group from 3 to 36h postfeeding. For acetate:propionate (Ac:Pr) ratio at 6, 12, 24, 27, 30 and 36 h postfeeding, the adapted group had greater values compared to the nonadapted group. For butyrate molar proportion at 0, 3, 6, 9, 12, 33 and 36h postfeeding the adapted group had greater values than the non-adapted group. For ammonia nitrogen (NH3- N) concentration at 6h, the non-adapted group had greater values than the adapted group (26.1 vs. 19.3, respectively), however at 9, 30, 33 and 36h postfeeding, the adapted group had higher values compared to the non-adapted group. It was also observed an interaction between time and additive (P=0.0430) for butyrate molar proportion, but when the analysis was performed by time no effect was observed. For the relative values of protozoa measured (Dasytricha, Isotricha, Epidinium, Diplodinium and Entodinium) only Entodinium presented adaptation effect (P<0.0236) with a higher proportion in the adapted group. Haptoglobin values was also not influenced (P>0.05) by additive or adaptation effect. Polyclonal antibodies preparation was not as effective as the gradual adaptation to the diet high concentrate to control changes of ruminal parameters.

Orientador: Elizabeth Moreira dos Santos Schmidt / Resumo: Este trabalho encontra-se dividido em três capítulos. O capítulo 1 versa sobre uma revisão de literatura que teve como objetivo a realização de um levantamento retrospectivo sobre os endoparasitas em bovinos no Brasil, que também incluiu dados mundiais. No capítulo 2, objetivou-se investigar os nematódeos gastrintestinais (GI) e pulmonares que parasitam bovinos jovens. Para isso, foram utilizados 140 bezerros mestiços, de dois a 12 meses de idade, clinicamente saudáveis, de duas propriedades leiteiras nos municípios de Botucatu e Manduri, estado de São Paulo. Durante um ano, trimestralmente, amostras de fezes foram coletadas diretamente da ampola retal para a realização da contagem de OPG pela técnica de McMaster modificada e pesquisa de L1 de Dictyocaulus viviparus pela modificação da técnica de Baermann. Além disso, coproculturas foram realizadas em pool de amostras para a observação das larvas infectantes. Na propriedade de Manduri, houve um aumento significativo da contagem de OPG no inverno. Na propriedade de Botucatu, os animais com até três meses de idade apresentaram maiores contagens de OPG quando comparados aos animais mais velhos. Em geral, foram observados ovos de estrongilídeos, Strongyloides spp., Moniezia spp., Trichuris spp. e oocistos de Eimeria spp. Nas coproculturas, em ordem de prevalência, foram recuperadas larvas infectantes de Cooperia spp., Haemonchus spp., Oesophagostomum spp. e Trichostrongylus spp. As L1 de D. viviparus foram recuperadas somente na ... (Resumo completo, clicar acesso eletrônico abaixo) / Abstract: This work was divided into three chapters. The chapter 1 aimed to perform a review article based on a retrospective survey of epidemiological studies examining endoparasites that affect bovine in different regions of Brazil, which also included international data. The chapter 2 aimed to determine the gastrointestinal (GI) and pulmonary parasites that affect calves. For this, we used 140 clinically healthy crossbred calves, two to 12 months old, which belonged to two private farms in the municipalities of Botucatu and Manduri, São Paulo state. The animals were monitored for 12 months. Feces were collected directly from the rectum every three months. Fecal egg counts were determined using the modified McMaster technique with a sensitivity of 50 eggs per gram of feces (EPG). Coproculture was performed on pooled samples to identify Strongylida infective larvae. First-stage larvae of Dictyocaulus viviparus were identified by a modified Baermann method. The calves from Manduri farm showed a significant increase in the fecal egg counts in the winter when to compare to other seasons. The Botucatu farm calves differed significantly in the age groups in that younger calves (2 – 3 months old) showed a higher EPG than older calves (8 – 12 months old). Generally, we found Strongyle type-eggs, Strongyloides spp., Moniezia spp., and Trichuris spp. eggs and Eimeria spp. oocysts. The most prevalent genera in all coprocultures were: Cooperia spp., Haemonchus spp., Oesophagostomum spp., and Tri... (Complete abstract click electronic access below) / Mestre

Com este estudo objetivou-se avaliar os níveis de estresse e a qualidade de carne de cordeiros mestiços Santa Inês x Dorper, submetidos a transporte de percurso curto (duas horas) e longo (seis horas) e em dois períodos de espera pré-abate (12 ou 24 horas). Foram utilizados 32 cordeiros com 127 ± 7 dias de idade e 30,4 ± 2,1 kg de peso vivo. Antes de serem submetidos aos períodos de transporte, os animais estavam alocados em baias coletivas e receberam ração concentrada (farelo de soja e milho, calcário calcítico e núcleo com monensina), bagaço úmido de citros e capim Napier (Pennisetum purpureum) e água ad libitum. Foram realizadas avaliações de reatividade dos animais no momento do embarque, desembarque e durante a contenção dos animais para as colheitas de sangue, urina e temperatura ocular por termografia infravermelho. Durante o período de espera pré-abate, realizou-se a colheita de dados comportamentais dos animais. Foram avaliadas as concentrações de cortisol no soro, na urina e as concentrações de haptoglobina no soro nos períodos que antecederam a insensibilização, assim como no momento do abate. Os animais apresentaram baixa reatividade durante todos os manejos. Houve diferença significativa no comportamento dos cordeiros durante os períodos de espera (P < 0,05) que durante as 12 horas apresentaram frequência de comportamentos que indicaram bem-estar favorável, enquanto que o período de transporte não afetou (P > 0,05). Os níveis de cortisol no soro mantiveram-se semelhantes da saída dos animais para o transporte até o final do período de espera (P > 0,05), enquanto houve oscilação dessas concentrações no cortisol na urina (P < 0,05), com pico no desembarque dos animais de duas horas de transporte e diminuição ao final do período de descanso. Os níveis de haptoglobina mantiveram-se semelhantes da colheita realizada antes do transporte, no embarque e no desembarque (P > 0,05) e diminuíram no final do período de espera pré-abate (P < 0,05). A temperatura ocular elevou-se no embarque e no desembarque dos animais, com diminuição da temperatura ao final do período de espera (P < 0,05). No momento do abate, foi observado aumento das concentrações de haptoglobina (P < 0,05), enquanto não houve alteração nas concentrações de cortisol no soro (P > 0,05). Animais que permaneceram por 24 horas de espera pré-abate apresentaram maior força de cisalhamento e menor luminosidade (L*) e intensidade de amarelo (b*). As variáveis comportamentais foram pouco afetadas pelos períodos de transporte e de espera pré-abate, porém o período de 12 horas de espera favoreceu a qualidade da carne / This study aimed to evaluate the stress levels and the meat quality from crossbred Santa Inês x Dorper lambs transported in short route (two hours) and long route (six hours) and maintained in two lairage periods (12 or 24 hours). Thirty-two crossbred Dorper x Santa Inês lambs with 127 ± 7 days of age and 30.4 ± 2.1 kg of body weight were used. Before the transport, the animals were allocated in collective pens and received concentrated feed (soy and corn meal, limestone and nucleus with monensin), wet bagasse citrus, Napier grass (Pennisetum purpureum) and water ad libitum. Reactivity evaluations were performed at loading, unloading and restraint of animals during the blood and urine collection and eye temperature by infrared thermography. During the pre-slaughter period (lairage), behavioral data were collected. Cortisol and haptoglobin serum concentrations and urine cortisol concentrations were measured in periods prior to stunning and at bleeding. The animals had low reactivity for all managements. The lairage periods changed the behavior of lambs (P < 0.05) with animals which have remained for 12 hours showed frequency of behaviors that indicate favorable welfare. The transport period did not affect (P > 0.05) the behavior of animals. The serum cortisol levels remained similar of the output for transport to the end of the lairage period (P > 0.05), while there was oscillation these concentrations in urine (p < 0.05) with peak on unloading in animals transported for two hours and a decrease at the end of the lairage period. The haptoglobin levels were similar between the collection performed before transportation, loading and unloading (P > 0.05) and decreased at the end of pre slaughter lairage (P <0.05). Ocular temperature rose at loading and unloading, with decreasing at the end of lairage period (P <0.05). At slaughter was observed increase of haptoglobin concentrations (P < 0.05), while there was no change in cortisol concentrations in serum (P> 0.05). Animals that remained for 24 hours lairaged before slaughter had higher shear force and lower luminosity (L *) and yellow intensity (b *). Behavioral variables were little affected by transport and lairage periods, but 12 hours of lairage favored the quality of meat

Le vieillissement est associé un mécanisme d'arrêt du cycle cellulaire nommé senescence. Au niveau de l'adipocyte, cet état cellulaire semble contribuer à la survenue d'altérations métaboliques et à un état pro-inflammatoire. Dans ce contexte, le tissu adipeux blanc viscéral pourrait jouer un rôle déterminant sur la perte du contrôle métabolique et ainsi participer à l'installation de pathologies associées au vieillissement. Au cours du vieillissement, le tissu adipeux blanc subit des modifications morphologiques et physiologiques conduisant à une altération progressive des fonctions de stockage et endocrines de l'adipocyte. Ainsi, un nouveau profil sécrétoire pro-inflammatoire appelé SASP (Senescence Associated Secretory Phenotype) a pu être mis en évidence et pourrait être impliqué dans la survenue de différentes pathologies liées à l'âge (diabète, insuffisance cardiaque ou rénales, ...). Dans ce sens, l'analyse précise du SASP d'adipocytes issus de souris de différents âges nous a permis d'identifier une cytokine pro-inflammatoire, l'haptoglobine, comme un nouveau candidat potentiellement impliqué dans les désordres métaboliques et inflammatoires associés au vieillissement. Nos premiers résultats montrent que, via une boucle de régulation, la senescence augmente la production d'haptoglobine adipocytaire et que réciproquement., cette production entretien la senescence de l'adipocyte Au niveau fonctionnel, l'haptoglobine altère les principales fonctions adipocytaires métaboliques telles que la lipolyse et la sensibilité à l'insuline. Des expériences sont en cours afin de confirmer in vivo l'importance de cette adipocytokine sur les altérations métaboliques entrainant une accélération du vieillissement de l'organisme. Cette étude permettra de mieux comprendre la participation de l'haptoglobine dans la perte des fonctions du tissu adipeux afin de développer de nouvelles stratégies thérapeutiques pour ralentir les processus de vieillissement. / Aging is associated with a cell cycle arrest mechanism named senescence. In adipocyte, this cell state could contribute to metabolic alterations as well as a low-grade inflammatory state. In this context, visceral white adipose tissue could play a major role in age-associated setup pathologies through the loss of metabolic control. Indeed, during aging, white adipose tissue undergoes functional and morphological modifications progressively leading to altered storage and endocrine capacities. Consequently, it has been hypothesized that a new emerging adipocyte secretory profile associated with aging (SASP for senescence associated secretory phenotype) could actively participate to the progressive onset of metabolic diseases related to aging. By proteomic analysis, we identified haptoglobin as a new proinflammatory cytokine overproduced by murine adipose tissue during aging. Our results showed a regulatory feedback loop between adipocyte haptoglobin and senescence state arguing for a role of the cytokine in aging process. Moreover, haptoglobin induced adipocyte metabolic alterations in vitro targeting lipolysis and insulin sensitivity. In vivo validation of haptoglobin's role on metabolic-induced aging are currently ongoing. Our study will allow a better understanding of haptoglobin's role in age-related adipose tissue loss of function and will pave the road for a new therapeutic strategy in the field of metabolism and age-associated pathologies.

<p>Haptoglobin (Hp) is a serum protein known for its ability to form a tight complex with hemoglobin (Hb) and thereby inhibiting the oxidative activity of Hb. </p><p>Mammalian Hp is synthesized as a precursor (proHp) that undergoes proteolytic cleavage by a previously unidentified enzyme in the endoplasmic reticulum (ER). In this study, a proHp-cleaving enzyme was isolated from human serum and identified as complement C1r-like protein (C1rLP). Co-expression of C1rLP with proHp in mammalian cells resulted in cleavage of the latter protein in the ER. Mutation of either the active site serine residue in C1rLP or the arginine residue in the cleavage site of Hp abolished the cleavage of proHp by C1rLP. RNAi studies in mammalian cells identified the proHp-cleaving enzyme as C1rLP.</p><p>Hp has been found in all mammals studied to date but its presence in non-mammalian species has not been unambiguously shown. By searching currently available genomic DNA and cDNA sequence databases, a gene orthologous to mammalian <i>Hp</i> was found in bony fish. Hp-like protein expressed from this gene was demonstrated to be a major Hb protein in fish serum. Surprisingly, no Hp-like gene was found in the genomes of either frog or chicken. In chicken, a protein previously described as Hp was identified as PIT54, a member of a scavenger receptor cysteine-rich family of proteins. Interestingly, ostrich serum seemed to contain two Hb-binding proteins; one similar to PIT54 and one to mammalian Hp. We are not aware of any other case where the function of one gene has been taken over by another, completely unrelated gene</p><p>Fish Hp (fHp) is composed of a serine proteinase-related domain preceded by an extension consisting of several aminoa acids and a signal peptide. The extension contains a consensus motif for cleavage by subtilisin-like proprotein convertases (SPCs). fHp was found to be cleaved by SPCs in the Golgi complex.</p><p>Collectively, this thesis presents evidence that Hp has undergone significant changes during evolution with respect to its molecular organization and to the mechanism of its proteolytic cleavage.</p>

THE TRYPANOLYTIC FACTOR OF HUMAN SERUM: A TROJAN HORSE African trypanosomes, the prototype of which is Trypanosoma brucei, are protozoan parasites of huge clinical, veterinary and economical importance. They develop in the body fluids of various mammals (including humans) where they face and manipulate many different aspects of the immune system. The extent of this interplay is pivotal to both host and parasite survival, and depending on parasite virulence and host susceptibility, infection duration ranges from some months to several years. At the end, host survival is invariably compromised. Humans and few other primates provide however a striking exception to this fatal outcome. They are indeed fully protected against most trypanosome infections through the presence in their blood of a so-called trypanosome lytic factor (TLF). The TLF is known to circulate mainly in the form of a high density lipoprotein particle characterized by the simultaneous presence of two primate-specific proteins: haptoglobin-related protein (Hpr) and apolipoprotein L-I (apoL-I). We have contributed to delineate the respective roles played by Hpr and apoL-I in the lysis process. ApoL-I was shown to be the exclusive toxin of the TLF. In its absence humans get fully susceptible to any trypanosome infection. The toxin was shown to kill the parasite after endocytosis through the generation of ionic pores in the lysosomal membrane. Those pores dissipate membrane potential and trigger the influx of chloride ions from the cytoplasm into the lysosomal compartment, leading to an eventually fatal uncontrolled osmotic phenomenon. ApoL-I efficient delivery to the parasite relies on Hpr. African trypanosomes indeed fulfil their heme nutritional requirements by receptor-mediated internalization of the complex formed by haptoglobin, an evolutionary conserved acute-phase protein, and hemoglobin, resulting from physiological intravascular hemolysis. This heme uptake by the auxotrophic parasites contributes to both growth rate and resistance against host oxidative burst. In human serum, the trypanosome receptor is unable to discriminate between Hp and the closely related TLF-bound Hpr, explaining TLF efficient endocytosis. As such, the TLF acts as a Trojan horse, killing the parasite from inside the cell after having deceived its vigilance through the high similarity between heme-delivering haptoglobin and toxin-associated Hpr.

Haptoglobin (Hp) is a serum protein known for its ability to form a tight complex with hemoglobin (Hb) and thereby inhibiting the oxidative activity of Hb. Mammalian Hp is synthesized as a precursor (proHp) that undergoes proteolytic cleavage by a previously unidentified enzyme in the endoplasmic reticulum (ER). In this study, a proHp-cleaving enzyme was isolated from human serum and identified as complement C1r-like protein (C1rLP). Co-expression of C1rLP with proHp in mammalian cells resulted in cleavage of the latter protein in the ER. Mutation of either the active site serine residue in C1rLP or the arginine residue in the cleavage site of Hp abolished the cleavage of proHp by C1rLP. RNAi studies in mammalian cells identified the proHp-cleaving enzyme as C1rLP. Hp has been found in all mammals studied to date but its presence in non-mammalian species has not been unambiguously shown. By searching currently available genomic DNA and cDNA sequence databases, a gene orthologous to mammalian Hp was found in bony fish. Hp-like protein expressed from this gene was demonstrated to be a major Hb protein in fish serum. Surprisingly, no Hp-like gene was found in the genomes of either frog or chicken. In chicken, a protein previously described as Hp was identified as PIT54, a member of a scavenger receptor cysteine-rich family of proteins. Interestingly, ostrich serum seemed to contain two Hb-binding proteins; one similar to PIT54 and one to mammalian Hp. We are not aware of any other case where the function of one gene has been taken over by another, completely unrelated gene Fish Hp (fHp) is composed of a serine proteinase-related domain preceded by an extension consisting of several aminoa acids and a signal peptide. The extension contains a consensus motif for cleavage by subtilisin-like proprotein convertases (SPCs). fHp was found to be cleaved by SPCs in the Golgi complex. Collectively, this thesis presents evidence that Hp has undergone significant changes during evolution with respect to its molecular organization and to the mechanism of its proteolytic cleavage.

Die vorliegende Arbeit überprüft vergleichend die Wirksamkeit der NSAID Flunixin und Ketoprofen in der unterstützenden Therapie bei Rindern nach Schwergeburten auf hämatologische und klinisch-chemische Parameter. Sie fokussiert sich dabei auf Parameter, welche für die Einschätzung einer endotoxämischen Belastung von Relevanz sind. Besondere Bedeutung kommt hierbei dem Haptoglobin und dem CRP zu, da bislang keine Untersuchungen zum Einfluss von NSAID auf Akute-Phase-Proteine beim Rind vorliegen.

Ziel der Arbeit war es festzustellen, ob Haptoglobin, ein Akute-Phase-Protein der Hauswiederkäuer, auch bei verschiedenen Wildwiederkäuern und Elefanten in vergleichbarer Weise fungiert und somit als Entzündungsmarker genutzt werden kann. Zusammenfassend kann aus den Ergebnissen dieser Untersuchungen geschlossen werden, dass bei Wildwiederkäuern die Bestimmung des Hp zur Detektion und Beurteilung der Behandlung entzündlicher Krankheitsprozesse genutzt werden kann.

Made available in DSpace on 2014-06-11T19:26:19Z (GMT). No. of bitstreams: 0 Previous issue date: 2008-08-14Bitstream added on 2014-06-13T19:54:31Z : No. of bitstreams: 1 alegranci_p_me_arafcf.pdf: 321277 bytes, checksum: 8ff505ee7ecdc73407004d69bb62d3b8 (MD5) / Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) / Universidade Estadual Paulista (UNESP) / A haptoglobina é uma glicoproteína plasmática sintetizada principalmente pelo fígado com atividade antioxidante e imunomodulatória. Sua principal função é a formação de um complexo estável com a hemoglobina livre, prevenindo a excreção de ferro pelos rins e danos causados pelo efeito oxidativo do mesmo. O polimorfismo desta proteína é caracterizado por três genótipos principais: Hp1 / Hp1, Hp2 / Hp1 e Hp2 / Hp2, sendo que estes apresentam subtipos na dependência das recombinações entre os alelos Hp1F, Hp1S, Hp2FS, Hp2SF, Hp2FF e Hp2SS. Apesar de contraditório, vários autores tentam correlacionar esses tipos e subtipos com doenças, na tentativa de responsabilizar algum deles como facilitador ou conferir resistência quanto às mesmas. Os objetivos deste estudo foram verificar as freqüências desse polimorfismo, suas freqüências alélicas e possíveis correlações em portadores e não portadores de doença arterial coronariana, bem como em doadores de sangue, pertencentes a uma parcela da população paulista. Dessa forma foram analisados 125 pacientes com DAC, 69 com ausência de DAC e 124 doadores de sangue. O material genético foi extraído, amplificado e após restrição enzimática com Dra I permitiu identificar os tipos e subtipos da haptoglobina. Os resultados permitiram concluir que essa caracterização pela técnica aplicada mostrou ser rápida e de fácil realização. Os portadores de DAC e os doadores de sangue evidenciaram maior freqüência do Hp2 / Hp2 e aqueles com ausência de DAC do genótipo heterozigoto, não sendo observada diferenças estatísticas significativas entre eles. Essa mesma observação era verificada quando estratificávamos os grupos quanto aos sexos. A freqüência alélica do Hp2 se mostrou maior que o Hp1 para os três grupos analisados. A distribuição dos subtipos da haptoglobina revelou que o prevalente... / Haptoglobin is a glycoprotein synthesized by liver with antioxidant and immunomodulatory properties. Its main function is the formation of a stable complex with free hemoglobin, preventing iron loss and damage caused by oxidative effects. The polymorphism of this protein is characterized by three main genotypes: Hp1 / Hp1, Hp2 / Hp1 e Hp2 / Hp2, and these have subtypes in dependence of the recombination among the alleles Hp1F, Hp1S, Hp2FS, Hp2SF, Hp2FF e Hp2SS. Despite contradictory, the authors try to associated types and subtypes with diseases, attempt to hold responsible for someone or to permit resistance to them. The purposes of our study were to find the frequencies of this polymorphism, their allelic frequencies and possible associations in patients with coronary artery disease and patients without coronary artery disease, as well as in blood donors, from one fraction of Brazilian population. We have analyzed 125 patients with CAD, 69 patients without CAD and 124 blood donors. Genomic DNA was extracted, amplified and after enzyme restriction with Dra I we could identify types and subtypes of haptoglobin. With the results we conclude that this applied technique is fast and it’s easy to carry out. Patients with CAD and blood donors evidenced higher frequencies for Hp2 / Hp2 and patients without CAD from heterozygous genotypes, weren’t observed significant statistical differences among them. The same observation was verified when we stratified the groups regarding genders. The allelic frequency of Hp2 was shown higher than Hp1 for the three groups analyzed. The distribution of haptoglobin subtypes revealed that the prevalent for all groups were represented by Hp2FS / Hp2FS, followed by Hp2FS / Hp1F in patients with CAD and without CAD and Hp2FF / Hp2FF in blood donors. The third in expression was represented by Hp1S / Hp1S in patients with CAD, Hp2FS / Hp1S in patients without...(Complete abstract click electronic access below)