Düsseldorf, Universiẗat, Diss., 2002.

Orientador : Ana Maria Lima de Azeredo-Espin / Dissertação (mestrado) - Universidade Estadual de Campinas, Instituto de Biologia / Made available in DSpace on 2018-08-02T14:54:27Z (GMT). No. of bitstreams: 1 Lemos_TailaAndrade_M.pdf: 3951909 bytes, checksum: 3f2ba88513abb52f3e6defc4445d311f (MD5) Previous issue date: 2000 / Resuimo: Dermatobia hominis (Linnaus Jr.) conhecida popularmente como mosca do berne. É uma das principais causadoras de miíase primária em vertebrados. sendo uma das principais pragas da pecuária nacional. causando grandes prejuízos à industria coureira. laticínios e frigoríficos. A mosca do berne apresenta uma biologia interessante e ciclo de vida bastante peculiar. o que dificuta a observação da espécie na natureza e a manutenção desta em laboratório. Por estes motivos. a caracterização da variabilidade genética e da estrutura populacional são importantes ferramentas na compreensão da evolução de D. hominis. Para este fim. foram utilizadas as técnicas de RAPD, com seis populações, e de RFLP do DNA mitocondrial, com doze populações. Os resultados foram comparados entre si e com estudos anteriores. Além disso, foram comparadas diferentes abordagens que podem ser aplicadas aos dados obtidos com RAPD. Os resultados de RAPD e RFLP do DNA mitocondrial sugerem que a espécie comporta-se como uma metapopulação. na qual as populações são altamente variáveis e apresentam fluxo gênico entre si. Quanto às diferentes abordagens aplicáveis aos dados de RAPD foi observado que as medidas de Dice e Jaccard, calculadas a partir da matriz completa. e a distância de Nei (1972) calculada com as modificações de Lynch & Milligan (1994), foram as mais adequadas ao estudo da variabilidade de D. hominis. Com o uso do RFLP do mtDNA foram detectados setenta e dois baplótipos, o que denota alta variabilidade para a espécie / Abstract: Dermatobia hominis (Linnaus Jr.), the human bot fly, is one of the most important agent of primary myiases in vertebrates and one of the most important pests of the national cattle breeding and causes large losses in the industry of leather, milk and meat. The bot fly has an interesting biology and a very peculiar life cycle. These facts make difficult to observe the specie in nature and to breed it in laboratory. Due to these reasons, the characterization of the genetic variability and structure are important tools in the understanding of the D. hominis evolution. Techniques based on RAPD, with six populations, and RFLP of mitochondrial DNA, with twelve populations were applied for this purpose. Estimates of several biometrical procedures applied to the data were compared. Comparisons were also made with results of previous studies. Different approaches for analyzing RAPD data were also compared. The results of RAPD and mtDNA suggest that the specie behave as a metapopulation whose populations are variable and present gene flow among themselves. Results of different methods based on RAPD indicated that the Dice and Jaccard measures, calculated :from the complete matrix of data, and of Nei distances (1972) with the modifications of Lynch & Milligan (1994) were more adequate for the study of the variability of D. hominis. With the use of rntDNA RFLP, seventy-two haplotypes were detected, indicating high variability for this specie / Mestrado / Genetica Animal e Evolução / Mestre em Genética e Biologia Molecular

Les mycoplasmes sont des bactéries à petit génome dérivées d’ancêtres à Gram positif par une succession de pertes de matériel génétique. Il a longtemps été considéré que la réduction génétique était la seule force régissant l’évolution de ces bactéries, cependant, des transferts horizontaux de grandes régions chromosomiques au sein et entre les espèces de mycoplasmes ont été récemment mis en évidence. Des éléments conjugatifs et intégratifs (ICE) découverts chez certaines espèces de mycoplasme pourraient être à l’origine de ces transferts. Ces ICEs codent les systèmes nécessaires pour leur excision, leur transfert conjugatif et leur intégration dans la cellule receveuse.Mycoplasma hominis est un mycoplasme commensal des voies génitales qui peut être responsable d’infections gynécologiques, d’infections néonatales et d’infections extragénitales. L’analyse préliminaire de génomes de M. hominis avait montré la présence de régions codantes caractéristiques des ICEs. Les objectifs de cette thèse étaient de rechercher et caractériser les ICEs chez 12 isolats cliniques de M. hominis entièrement séquencés et de déterminer la prévalence de ces ICEs au sein de l’espèce M. hominis. Pour cela, une étude rétrospective sur une période de 6 ans a été menée sur des isolats cliniques obtenus au CHU de Bordeaux. Les concentrations minimales inhibitrices des tétracyclines et des fluoroquinolones ainsi que les mécanismes de résistance ont été déterminés, permettant de disposer d’une collection d’isolats cliniques caractérisés pour l’étude des ICEs.Des ICEs de près de 30 kpb ont été trouvés en une ou plusieurs copies dans sept des 12 souches de M. hominis séquencées. Seulement cinq de ces ICEs semblaient fonctionnels puisqu’une forme circulaire a pu être détectée. Tous les ICEs de M. hominis présentaient une structure similaire avec un module spécifique de M. hominis d’environ 4-kpb, codant des protéines ayant des caractéristiques structurelles similaires à des effecteurs TAL (transcription activator-like), impliqués dans la reconnaissance de nucléotides et dans la transduction de signaux chez les bactéries symbiotiques. La caractérisation des mécanismes de résistance aux antibiotiques des isolats cliniques de M. hominis collectés au CHU de Bordeaux nous a permis de disposer d’une collection de 183 isolats isolés entre 2010 et 2015, parmi lesquels 14,8% étaient porteur du gène tet(M) responsable de la résistance aux tétracyclines, 2,7% étaient résistant à la lévofloxacine et 1,6% étaient résistants à la moxifloxacine par mutation des gènes de la topoisomérase IV et de l’ADN gyrase. Le screening de 120 de ces isolats cliniques a révélé une prévalence élevée des ICEs dans l’espèce M. hominis, mesurée à 45%. Il n’y avait pas de prédominance des ICEs dans les isolats portant le gène tet(M), suggérant que les ICEs n’étaient pas responsables de la dissémination de la résistance à la tétracycline.Des expériences complémentaires de conjugaison seront nécessaires pour confirmer la fonctionnalité des ICEs retrouvés dans l’espèce M. hominis. Cependant, la forte prévalence et le caractère très conservé des ICEs chez M. hominis suggèrent que ces ICEs pourraient conférer un avantage sélectif pour la physiologie ou la physiopathologie de la bactérie. Ce travail ouvre ainsi la voie à de futures études qui permettront une meilleure compréhension des transferts horizontaux de gènes et des facteurs de virulence chez M. hominis. / Mycoplasmas are small-genome bacteria derived from Gram-positive ancestors by a succession of genetic material losses. It has long been considered that genetic reduction was the only force governing the evolution of these bacteria, however, horizontal transfers of large chromosomal regions within and between mycoplasma species have recently been reported. Conjugative and integrative elements (ICE) found in some species of mycoplasma may be responsible for these transfers. These ICEs encode the systems necessary for excision, conjugative transfer and integration into a recipient cell.Mycoplasma hominis is a commensal genital mycoplasma that can be responsible for gynecological infections, neonatal infections and extragenital infections. Preliminary analysis of M. hominis genomes had showed the presence of coding regions characteristic of ICEs. The objectives of this thesis were to search for and characterize ICEs in one reference strain and 11 fully sequenced M. hominis clinical isolates and to determine the prevalence of these ICEs in the M. hominis species. To do so, a retrospective study over a period of 6 years was conducted on clinical isolates collected at the Bordeaux University Hospital. The minimum inhibitory concentrations of tetracyclines and fluoroquinolones as well as resistance mechanisms were determined, providing a collection of clinical isolates characterized for the study of ICEs.ICEs of 27-30 kpb were found in one or two copies in seven of the 12 M. hominis sequenced strains. Only five of these ICEs seemed functional since circular forms of extrachromosomal ICE were detected. All M. hominis ICEs exhibited a similar structure consisting of a 4.0-5.1 kb module composed of five to six juxtaposed CDSs, encoding proteins that share common structural features with transcription activator-like (TAL) effectors, involved in polynucleotide recognition and signal transduction in symbiotic bacteria. The characterization of antibiotic resistance mechanisms in M. hominis clinical isolates collected at Bordeaux University Hospital enabled us to obtain a collection of 183 isolates isolated between 2010 and 2015, of which 14.8% harbored the tet(M) gene responsible for tetracycline resistance, 2.7% were resistant to levofloxacin and 1.6% were resistant to moxifloxacin by mutation in topoisomerase IV and DNA gyrase genes. Screening of 120 of these clinical isolates revealed a high prevalence of ICEs in M. hominis, measured to be 45%. The proportion of ICEs was not higher in isolates carrying the tet (M) gene, suggesting that ICEs were not responsible for the spread of tetracycline resistance.Additional mating experiments will be necessary to confirm the functionality of the ICEs found in the M. hominis species. However, the conserved and specific structure of M. hominis ICEs and the high prevalence in clinical strains suggest that these ICEs may confer a selective advantage for the physiology or pathogenicity of the bacteria. This work opens the way for future studies that will provide a better understanding of horizontal gene transfers and virulence factors in M. hominis.

Objetivo: Determinar la prevalencia de infección por Strongyloides stercolaris y otras enteroparasitosis en el pueblo joven Santo Toribio de Mogrovejo de Chiclayo durante Junio-Octubre del 2011. Material y Métodos: Estudio descriptivo, trasversal; muestreo aleatorio, estratificado, polietápico, siendo el tamaño muestral de 106 pobladores. Se diseñó y validó una ficha de recolección epidemiológica. Un biólogo recibió entrenamiento en las técnicas de diagnóstico de Strongyloides stercolaris en un centro referencial de Lima. Se recolectaron 3 muestras por paciente, sometidas a 5 técnicas parasitológicas: Examen directo de heces, Baermann modificado en Copa por Lumbreras, Test de sedimentación espontánea, Cultivo en agar y Cultivo Dancescu. Resultados: Se visitaron 124 casas; el porcentaje de respuesta fue de 85,7%. Se logró entrevistar 106 personas. El promedio de edad fue de 27,8 +/- 16,9 años; hubieron 31 hombres (29,2%) y 75 mujeres (70,8%). El 26,4% de personas habían realizado un viaje a la Sierra y/o Selva en los últimos 5 años con una estancia mayor a un mes. El piso de tierra fue el más frecuente en el total de viviendas (55,6%); 102 personas (96,2 %) tenían desagüe; 23 pobladores (21,7 %) tuvieron al menos un parásito detectado. No se hallaron pobladores infectados con Strongyloides stercolaris. La enteroparasitosis más frecuente fue por protozoarios, con predominio de Blastocystis hominis en un 12,3%. Conclusiones: Se halló una baja frecuencia de enteroparasitosis y ausencia de pobladores infectados con Strongyloides stercolaris. El parásito más frecuente fue Blastocystis hominis. / Tesis

Människor har olika riskuppfattningar beroende på tidigare erfarenheter, kulturell bakgrund och kunskap. Riskuppfattningarna kan skilja sig så att vissa människor upplever en inträffad händelse som en kris, medan andra upplever samma sak som en allvarlig händelse. Vid en kris eller allvarlig händelse prioriteras inte allmänhetens uppfattningar eller åsikter när ansvariga aktörer och myndigheter arbetar för att hitta orsaken till händelsen. Eftersom allmänheten är en stor grupp i samhället och är de som drabbas vid en kris eller allvarlig händelse, vill vi med denna studie undersöka hur de uppfattade det vattenburna Cryptosporidiumutbrottet i Östersund som pågick från november 2010 och tre månader framåt. För att genomföra studien har vi analyserat kvantitativa data från vår egen surveyundersökning som har genomförts med hjälp av enkäter. Den teoretiska referensram som ligger till grund för uppsatsen behandlar riskuppfattning samt kopplingar till risksamhället. De resultat som vi fick fram av studien visar att det finns signifikanta skillnader mellan ålder och hur man påverkades av Cryptosporidiumutbrottet, då i synnerlighet yngre påverkades mer av parasiten. Vidare finns stöd för detta resultat i tidigare forskning, som understödjer resonemanget att framförallt yngre personer påverkas mer av Cryptosporidium än äldre. Nyckelord: Vattenburen smitta, Cryptosporidium hominis, parasitutbrott, riskuppfattning och risksamhälle.

Objetivo: Determinar la prevalencia de infección por Strongyloides stercolaris y otras enteroparasitosis en el pueblo joven Santo Toribio de Mogrovejo de Chiclayo durante Junio-Octubre del 2011. Material y Métodos: Estudio descriptivo, trasversal; muestreo aleatorio, estratificado, polietápico, siendo el tamaño muestral de 106 pobladores. Se diseñó y validó una ficha de recolección epidemiológica. Un biólogo recibió entrenamiento en las técnicas de diagnóstico de Strongyloides stercolaris en un centro referencial de Lima. Se recolectaron 3 muestras por paciente, sometidas a 5 técnicas parasitológicas: Examen directo de heces, Baermann modificado en Copa por Lumbreras, Test de sedimentación espontánea, Cultivo en agar y Cultivo Dancescu. Resultados: Se visitaron 124 casas; el porcentaje de respuesta fue de 85,7%. Se logró entrevistar 106 personas. El promedio de edad fue de 27,8 +/- 16,9 años; hubieron 31 hombres (29,2%) y 75 mujeres (70,8%). El 26,4% de personas habían realizado un viaje a la Sierra y/o Selva en los últimos 5 años con una estancia mayor a un mes. El piso de tierra fue el más frecuente en el total de viviendas (55,6%); 102 personas (96,2 %) tenían desagüe; 23 pobladores (21,7 %) tuvieron al menos un parásito detectado. No se hallaron pobladores infectados con Strongyloides stercolaris. La enteroparasitosis más frecuente fue por protozoarios, con predominio de Blastocystis hominis en un 12,3%. Conclusiones: Se halló una baja frecuencia de enteroparasitosis y ausencia de pobladores infectados con Strongyloides stercolaris. El parásito más frecuente fue Blastocystis hominis.

Orientador: Teresa Cristina Goulart de Oliveira Sequeira / Resumo: O presente trabalho foi desenvolvido com o objetivo de caracterizar os produtos de excreção/secreção (PE/S) de larvas de Dermatobia hominis, especialmente, no que se refere à atividade proteolítica desses produtos. Os PE/S foram obtidos de larvas de primeiro estágio (L1) cultivadas em laboratório e de larvas de segundo (L2) e terceiro (L3) estágios colhidas de bovinos parasitados. O perfil das proteínas foi obtido pela eletroforese dos PE/S em gel de poliacrilamida contendo sódio dodecil sulfato (SDS-PAGE) e a atividade proteolítica foi investigada utilizando gelatina, azocaseína e N-a-benzoil-arginina-nitroanilida (BAPNA) como substratos. Para caracterização das proteases foram realizados ensaios utilizando estes mesmos substratos, nos quais as amostras foram tratadas com os inibidores: PMSF, TPCK, TLCK, DCI, E-64, EDTA, Elastatinal, Leupeptina, Fenantrolina e Antipaína. Nos PE/S de L1 foram detectadas exclusivamente proteases com peso molecular aparente acima de 168 kDa, cuja inibição revelou pertencerem aos grupos das metalo-proteases e serina-proteases. Os zimogramas dos PE/S de L2 e L3 em géis copolimerizados com gelatina revelaram uma ampla faixa de atividade proteolítica, na qual estavam presentes proteases de alto, médio e baixo pesos moleculares aparentes. Nos ensaios realizados com inibidores de proteases, utilizando os três substratos, as proteases presentes em L2 e L3 foram identificadas como pertencentes ao grupo das serina-proteases, sendo em L3, predominantemente, do tipo tripsina. As alterações nos padrões de proteólise e nas características bioquímicas das proteases presentes nos três estágios larvais discuti- se à atividade das larvas em cada fase do seu desenvolvimento. / Abstract: In the present investigation the biochemical characteristics of the Dermatobia hominis larvae secretory/excretory products (PE/S) were analyzed mainly regard to their proteolytic activities. The PE/S of first-instar larvae were collected from larvae reared in the laboratory and of the second and third instars from larvae removed from infested cattle.The PE/S were tested in a sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) to identify their proteic profiles and the proteases activity were investigated using gelatine, azocasein, and N-a-benzoil-arginine-nitroanilide (BAPNA) as substrates. The proteases characterizations were performed by treating PE/S samples by the following proteases inhibitors: PMSF, TPCK, DCI, E-64, EDTA, Elastatinal, Leupeptine, Phenatroline, and Antipain. SDS-PAGE-Gelatin profiles of L1 PE/S revealed only proteases with molecular weight above 168 KDa whereas profiles from L2 and L3 PE/S revealed a high range of proteolytic activity, including high, medium and low molecular weight proteases. The assays performed with the protease inhibitors, and gelatin and azocasein as substrates, revealed that metalloprotease is the major class of proteases present in the PE/S of L1 besides the low content of serine-proteases. The major class of proteases present in the PE/S of L2 and L3 was characterized as serine-proteases, being in L3 predominantly of trypsin type. The changes in the proteolytic patterns and biochemical characteristics of the proteases found in all three instar larvae of D. hominis were with the larval activity in each phase of their development. / Mestre

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Mycoplasma hominis est un pathogène humain opportuniste responsable d’infections génitales et néo-natales. Modifier génétiquement cette bactérie est nécessaire afin de comprendre les mécanismes de virulence et d’infection de ce pathogène. Il n’existe à ce jour aucun outil moléculaire efficace permettant de manipuler le génome de M. hominis, limitant les recherches sur sa pathogénicité et son métabolisme particulier reposant sur l’arginine. De nouvelles technologies rassemblées sous le terme de Biologie de Synthèse (BS) ont récemment émergé, offrant des perspectives inédites pour l’étude des mycoplasmes en permettant de modifier leurs génomes à grande échelle et de produire des souches mutantes. Ces travaux menés au J. Craig Venter Institute (JCVI, USA) ont montré que le génome de mycoplasmes apparentés pouvait être cloné et manipulé dans la levure avant d’être transplanté dans une cellule receveuse. La levure sert d’hôte d’accueil temporaire pour modifier le génome de la bactérie. Cette approche novatrice ouvre de nombreuses perspectives dans le cadre du développement de la génomique fonctionnelle chez les mycoplasmes pour lesquels les outils génétiques efficaces sont peu nombreux. Le but de cette thèse a été d’adapter pour la première fois certains outils de BS à M. hominis dans le but de créer des mutants déficients pour une fonction donnée. Pour cela, le génome de la souche type de M. hominis PG21 (665 kb) a été cloné dans la levure Saccharomyces cerevisiae par « Transformation-Associated Recombination cloning » (TAR-cloning). Deux clones (B3-2 et B3-4) de levure possédant le génome complet de M. hominis ont été validés par analyse en PCR simplex, PCR multiplex et électrophorèse en champs pulsé (PFGE). Ces clones levures ont ensuite été propagés en milieu sélectif durant 180 générations (30 passages), afin d’évaluer la stabilité du génome bactérien dans son hôte. Cette expérience a montré que (i) si la taille du génome de M. hominis ne variait pas au cours des premiers passages, elle diminuait progressivement à partir du dixième passage (≈60 générations), et que (ii) les zones du génome enrichies en séquence répétées étaient préférentiellement perdues. En tenant compte de ces résultats, le génome de M. hominis a été modifié chez le clone B3-4 par la technique « Clustered Regularly Interspaced Short Palindromic Repeats/Cas9 » (CRISPR/Cas9) lors de passages précoces. Des clones de S. cerevisiae possédant un génome de M. hominis PG21 complet délété du gène vaa, codant une protéine d’adhésion majeure, ont été ainsi produits. La dernière étape de cette approche consistait à transplanter le génome modifié dans une cellule receveuse de M. hominis ou de Mycoplasma arthritidis, espèce phylogénétiquement la plus proche de M. hominis. Aucun protocole de transformation de M. hominis n’étant disponible au début de nos travaux, cette étape constituait un verrou majeur dans la mise en place des outils de BS chez cette espèce. Ce verrou a été en partie levé puisqu’une méthode de transformation de M. hominis basée sur du polyéthylène glycol (PEG) et mettant en jeu le plasposon pMT85 (plasmide contenant un transposon conférant la résistance à la tétracycline) a été mise au point au laboratoire. Cette technique de transformation, développée pour la souche de référence M. hominis M132 (745 kb) reste encore peu efficace ; elle est néanmoins reproductible et a permis d’obtenir des mutants d’intérêt de M. hominis. Le transformant n°28-2 a, ainsi, été muté dans le gène Mhom132_2390, codant le précurseur de la protéine P75, une adhésine putative de M. hominis. Le séquençage des génomes complets d’autres transformants a révélé l’insertion de multiples copies du transposon et la présence d’évènements de duplication et d’inversion de larges fragments d’ADN dans au moins deux génomes de M. hominis. / Mycoplasma hominis is an opportunistic human pathogen responsible for genital and neonatal infections. Genetically modifying this bacterium is necessary to understand the virulence and infection mechanisms of this pathogen. There is currently no effective molecular tool to engineer the genome of this bacterium, limiting research on its pathogenicity and its peculiar metabolism based on arginine.New technologies have recently emerged in the field of Synthetic Biology (BS), offering new perspectives for the study of mycoplasmas by allowing large scale genome modifications and the production of mutant strains. Work at the J. Craig Venter Institute (JCVI, USA) has shown that the genome of related mycoplasmas can be cloned and manipulated in yeast before being transplanted into a recipient cell. The yeast serves as a temporary host to modify the genome of the bacterium. This innovative approach opens many perspectives in the development of functional genomics in mycoplasmas for which there are few effective genetic tools. The goal of this thesis was to adapt a number of BS tools to M. hominis for the first time, in order to create mutants deficient for a given function. To achieve this goal, the genome of the M. hominis type strain PG21 (665 kb) was cloned into the yeast Saccharomyces cerevisiae by Transformation-Associated Recombination cloning (TAR-cloning). Two yeast clones (B3-2 and B3-4) possessing the complete genome of M. hominis were validated by simplex PCR, multiplex PCR and Pulsed Field Gel Electrophoresis (PFGE) analyses. These yeast clones were then propagated in a selective medium for 180 generations (30 passages) to evaluate the stability of the bacterial genome in its host. This experiment showed that (i) the size of the genome of M. hominis did not change during the first passages, it decreased progressively from the tenth passage (≈60 generations), and (ii) the enriched genome areas in repeated sequence were preferentially lost. Thus, the genome of M. hominis was modified in the B3-4 clone at early passages using the Clustered Regularly Interspaced Short Palindromic Repeats/Cas9 (CRISPR/Cas9) technology. Yeast clones with a complete M. hominis PG21 genome with a deleted vaa gene, encoding a major adhesion protein, were produced using this approach. The final step of this approach was to transplant the modified genome into a recipient cell of M. hominis or Mycoplasma arthritidis, the species phylogenetically closest to M. hominis. As no M. hominis transformation protocol was available at the beginning of our work, this step constituted a major obstacle in the implementation of BS tools in this species. This barrier has been partially lifted since a method of transformation of M. hominis based on polyethylene glycol (PEG) and involving the plasposon pMT85 (plasmid carrying a transposon conferring resistance to tetracycline) has been developed in the laboratory. This transformation technique, developed for the reference strain M. hominis M132 (745 kb) still remains not very efficient; it is nevertheless reproducible and allowed to obtain M. hominis mutants of interest. The Mhom132_2390 gene, encoding the precursor of the P75 protein, a putative adhesin of M. hominis, was effectively mutated in transformant No. 28-2. Complete genome sequencing of other transformants revealed the insertion of multiple copies of the transposon and the presence of duplication and inversion of large DNA fragments within at least two M. hominis genomes.In conclusion, this data has opened the way for the development and transposition of existing genetic modification approaches to M. hominis, previously considered as a genetically intractable bacterium.

Made available in DSpace on 2016-04-28T20:16:27Z (GMT). No. of bitstreams: 1 2008 - Fabio Silva de Souza.pdf: 3760278 bytes, checksum: d38f28c39076f3f01e4393fd0f31bc10 (MD5) Previous issue date: 2008-11-26 / The aim of this study was to characterize the association between the seasonality of dermatobiosis in bovines and its dipterans potential vectors as well as evaluate the association among the counts of Dermatobia hominis larvae and of potential vectors with meteorologicals data. It also had the objective of delimiting, quantifying and characterizing the geographical space of Serop?dica and Itagua? municipalities in relation to the favorable degrees for the occurrence of dermatobiosis in bovines in the rainy and dry periods using the Vista SAGA? 2007 information system. The seasonal fluctuation of D. hominis larvae was obtained by counts in ten bovines and of muscoids dipterans by capture using Adultrap? traps with sardine bait, once a month each, in three properties named A, B and C, localized in the Serop?dica, Paracambi and Itagua? municipalities, respectively, in the period of January 2006 to December 2007. The meteorological data of compensated mean temperature, pluvial precipitation and relative humidity were obtained in the Agricultural Ecology Station (22?48'S, 43?41'W and 33m of altitude). The associations between D. hominis larvae fluctuations, adult dipterans and climatological data were verified using the Spearman correlation test. Kruskal-Wallis and Mann-Whitney tests were used in the analysis of the significant differences between the rainy and dry periods in each property in the monthly average of D. hominis larvae, in the total monthly number and of dipterans family and in the analysis of other studies. In the elaboration of models by geoprocessing, some abiotic factors were used: occupation and covering of the land, altitude, declivity, soils and climatic factors, in which the evaluation, signature and monitorship functions of the Vista SAGA? 2007 program were applied. The infestation by D. hominis larvae in bovines was present during the 24 months of study in the three properties. A total of 18.966 dipterans potential vectors was captured, being 30,16% in the A property, 34,59% in the B property and 35,25% in the C property but there were no significant statistic difference between these. There was a positive and significant correlation (rs=0,63) between monthly averages of D. hominis larvae and relative humidity in 2006 at the A property; there was a positive and significant correlation between the total number of captured dipterans and pluvial precipitation (rs=0,80) and temperature (rs=0,60) at the B and C properties, respectively, in 2007. It was also observed a positive and significant correlation between the total number of captured dipterans of the Sarcophagidae family (rs=0,60) with the temperature in the C property in 2006, and between Muscidae (rs=0,67) and Calliphoridae (rs=0,76) with pluvial precipitation in the B property in 2007. There wasn t an association between monthly averages of D. hominis larvae and dipterans. It was verified a pattern lack in the association between monthly and periods fluctuations, rainy and dry of D. hominis larvae with the dipterans potential vectors fluctuations and of both with meteorologicals data. The geoprocessing allowed delimiting, quantifying and characterizing the potential of space temporal distribution of dermatobiosis in bovines. / Este estudo teve como objetivos caracterizar a associa??o entre a sazonalidade da dermatobiose em bovinos e seus d?pteros potenciais vetores, assim como avaliar a associa??o entre as contagens de larvas de Dermatobia hominis e de potenciais vetores com os dados meteorol?gicos. Teve tamb?m como objetivo, delimitar, quantificar e caracterizar o espa?o geogr?fico dos munic?pios de Serop?dica e Itagua? quanto aos gradientes de favorabilidade para a ocorr?ncia da dermatobiose em bovinos nos per?odos chuvoso e seco utilizando o sistema de informa??o Vista SAGA? 2007. A flutua??o sazonal de larvas de D. hominis foi obtida por contagens em dez bovinos e a de d?pteros musc?ides por captura, usando armadilhas Adultrap? com isca de sardinha, ambos uma vez ao m?s, nas tr?s propriedades denominadas A, B e C, localizadas nos munic?pios de Serop?dica, Paracambi e Itagua?, respectivamente, no per?odo de janeiro de 2006 a dezembro de 2007. Os dados meteorol?gicos de temperatura m?dia compensada, precipita??o pluvial e umidade relativa, foram obtidos na Esta??o Ecologia Agr?cola (22?48'S, 43?41'W e 33m de altitude). As associa??es entre as flutua??es de larvas de D. hominis, d?pteros adultos e dados climatol?gicos foram verificadas utilizando-se o teste de correla??o de Spearman. Os testes de Kruskal-Wallis e Mann-Whitney foram empregados na an?lise das diferen?as significativas entre os per?odos, chuvoso e seco, por propriedade, para a m?dia mensal de larvas de D. hominis, o n?mero mensal total e por fam?lia de d?pteros e na an?lise de dados publicados em outros estudos. Para a elabora??o dos modelos por geoprocessamento, utilizaram-se os fatores abi?ticos: uso e cobertura das terras, altitude, declividade, solos e fatores clim?ticos, aplicando-se as fun??es avalia??o, assinatura e monitoria do programa Vista SAGA? 2007. A infesta??o por larvas de D. hominis em bovinos esteve presente ao longo dos 24 meses de estudo nas tr?s propriedades. Um total de 18.966 d?pteros potenciais vetores foi capturado. Deste, 30,16% foram capturados na propriedade A, 34,59% na propriedade B e 35,25% na propriedade C, mas sem diferen?a estat?stica significativa entre estas. Houve correla??o positiva e significativa (rs=0,63) entre as m?dias mensais de larvas de D. hominis e umidade relativa, no ano de 2006, na propriedade A; houve correla??o positiva e significativa entre o n?mero total de d?pteros capturados e precipita??o pluvial (rs=0,80) e tamb?m com a temperatura (rs=0,60) nas propriedades B e C, respectivamente, no ano de 2007. Observou-se tamb?m correla??o positiva e significativa entre o n?mero total de d?pteros capturados da fam?lia Sarcophagidae (rs=0,60) com a temperatura na propriedade C no ano de 2006 e entre Muscidae (rs=0,67) e Calliphoridae (rs=0,76) com precipita??o pluvial na propriedade B em 2007. N?o ocorreu associa??o entre as m?dias mensais de larvas de D. hominis e de d?pteros. Constatou-se uma falta de padr?o na associa??o entre as flutua??es mensais e por per?odos, chuvoso e seco, das larvas de D. hominis com as flutua??es de d?pteros potenciais vetores e de ambas com os dados meteorol?gicos. O geoprocessamento permitiu delimitar, quantificar e caracterizar o potencial de distribui??o espa?o-temporal da dermatobiose nos per?odos chuvoso e seco.