Thesis (MScEng (Process Engineering))--University of Stellenbosch, 2011. / ENGLISH ABSTRACT: Hydrocyclones are separation devices that are widely used throughout the chemical engineering and mineral processing industries. Although simple in design, the intricate flow structure of the device complicates control. As an alternative to conventional empirical and theoretical modelling, process state monitoring methods have recently been employed as a means to control hydrocyclones. The purpose of process state monitoring methods is to distinguish between the desired operating state with favourable separation, the transition state, and the troublesome operating state of dense flow separation. In comparison to previously employed monitoring techniques, image analysis of the underflow is regarded as a promising approach. Preliminary studies have indicated that the technique complies with hydrocyclone monitoring requirements: sensitivity, non-invasiveness, sampling times less than one second, robustness and low cost. The primary objective of this study was therefore defined as investigating the feasibility of image analysis of hydrocyclone underflow as a monitoring technique. Data collection entailed the recording of hydrocyclone underflow for different operating states. Six case studies were performed in total: Gold, Ilmenite, Platreef, Merensky 1, Merensky 2 and Merensky 3 (with the case study names indicating the different ore types used). An image analysis technique, consisting of feature extraction through motion detection, as well as various noise reduction methods, was consequently developed and applied to the video data. Classification of the various operating states was attempted by performing modelling by one-class support vector machines (SVM). Results indicated that the developed image analysis technique effectively addresses background noise, random noise and system vibration through image enhancement and a motion threshold. Extremely low contrast differences and foreground noise did, however, prove problematic in Ilmenite and Merensky 1 case studies respectively. For the remaining case studies, it was found that the various operating states were identified with high accuracy through one-class SVM classification. This is particularly true for the identification of the troublesome dense flow separation for which extremely low missing alarm rates were obtained (0 % in most cases). In terms of practicality, the technique proved to be sensitive, non-intrusive and economical. The sampling time of 30 frames per second and estimated processing to video time ratio of 1:1, is furthermore satisfactory. Ultimately, the results indicate that the image analysis of hydrocyclone underflow is a viable monitoring technique. The robustness of the technique might further be improved by use of backlighting and an air-knife. It is also recommended that future work should focus on testing the monitoring technique on an industrial hydrocyclone setup. / AFRIKAANSE OPSOMMING: Hidrosiklone is skeidingsapparate wat algemeen gebruik word in chemiese ingenieurswese en mineraalprosesserings industrieë. Alhoewel die apparaat ‘n eenvoudige ontwerp het, bemoeilik die komplekse interne vloeistruktuur die beheer daarvan. Prosestoestandmoniteringsmetodes is vir hidrosikloonbeheer toegepas as alternatief vir konvensionele empiriese en teoretiese modellering. Die doel van prosestoestandmoniteringsmetodes is om te onderskei tussen die gewenste bedryfstoestand met gunstige skeiding, die oorgangstoestand, en die moeilike bedryfstoestand van digtevloeiskeiding. In vergelyking met vorige toegepaste moniteringstegnieke, word beeldverwerking van die ondervloei beskou as ‘n belowende tegniek. Voorlopige studies het aangedui dat die tegniek voldoen aan die hidrosikloonmoniteringvereistes: sensitiwiteit, nie-indringendheid, monsternemingstydperke laer as een sekonde, robuustheid en lae koste. Die primêre doelwit van hierdie studie is daarom gedefineer as die ondersoek van die doenlikheid van beeldverwerking van hidrosikloon ondervloei as ‘n moniteringstegniek. Die data versameling het die afneem van hidrosikloon ondervloei vir verskillende bedryfstoestande behels. Ses gevallestudies is in totaal uitgevoer: Goud, Ilmeniet, Platreef, Merensky 1, Merensky 2 en Merensky 3 (die gevallestudie name dui die verskillende erts tipes wat gebruik is aan). ‘n Beeldverwerkingstegniek, wat bestaan uit kenmerkekstraksie deur bewegingsopsporing, asook verskeie geruisverlagingsmetodes, is gevolglik ontwikkel en toegepas op die video data. Klassifikasie van die verskeie bedryfstoestande is beproef deur modellering met enkelklassteunvektormasjiene. Resultate het aangedui dat die ontwikkelde beeldverwerkingstegniek agtergrond geruis, onreëlmatige geruis en sisteem vibrasie suksesvol aanspreek deur beeldversterking en ‘n bewegingslimiet. Beduidende lae kontrasverskille en voorgrond geruis blyk wel problematies in die Ilmeniet en Merensky 1 gevallestudies onderskeidelik. Vir die orige gevallestudies is gevind dat die verskillinde bedryfstoestande met hoë akkuraatheid geïdentifiseer is deur enkelklassteunvektormasjiene klassifisering. Dit is veral waar vir die identifisering van die moeilike digtevloeiskeiding waarvoor beduidende lae vermiste-alarmmaatstawwe behaal is (0 % in die meeste gevalle). Aangaande die praktiese aspekte, blyk die tegniek sensitief, nie-indringend en ekonomies. Die monsternemingstydperk van 30 raampies per sekonde en die beraamde prosesserings- tot videotyd verhouding van 1:1, is ook voldoende. Ten slotte dui die resultate daarop dat die beeldverwerking van hidrosikloon ondervloei ‘n uitvoerbare moniteringstegniek is. Die robuustheid van die tegniek sou verder verbeter kon word deur gebruik te maak van agtergrondverligting en ‘n lugspuit. Dit word ook aanbeveel dat toekomstige werk op die toetsing van die moniteringstegniek op ‘n industriële hidrosikloon toestel moet fokus.

Medical imaging has seen a rapid development in its clinical use in assessment of treatment outcome, disease monitoring and diagnosis over the last few decades. Yet, the vast amount of available image data limits the practical use of this potentially very valuable source of information for radiologists and physicians. Therefore, the design of computer-aided medical image analysis is of great importance to imaging in clinical practice. This thesis deals with the problem of deformable image registration in the context of lung imaging, and addresses three of the major challenges involved in this challenging application, namely: designing an image similarity for multi-modal scans or scans of locally changing contrast, modelling of complex lung motion, which includes sliding motion, and approximately globally optimal mathematical optimisation to deal with large motion of small anatomical features. The two most important contributions made in this thesis are: the formulation of a multi-dimensional structural image representation, which is independent of modality, robust to intensity distortions and very discriminative for different image features, and a discrete optimisation framework, based on an image-adaptive graph structure, which enables a very efficient optimisation of large dense displacement spaces and deals well with sliding motion. The derived methods are applied to two different clinical applications in pulmonary image analysis: motion correction for breathing-cycle computed tomography (CT) volumes, and deformable multi-modal fusion of CT and magnetic resonance imaging chest scans. The experimental validation demonstrates improved registration accuracy, a high quality of the estimated deformations, and much lower computational complexity, all compared to several state-of-the-art deformable registration techniques.

Characterizing nanoparticles to be able to understand how they functions in the body is important for development of drugs. Furthermore with increasing number of nanoparticle product the nanotoxicity of nanoparticles is important to understand. This report is a part of the EU-project Nanoclassifier which purpose is to “develop a cost effective, high throughput screening platform for characterization of the bionanointerface and its cell-binding partners”. Single-cycle kinetic was used to determine the number of binding epitopes on polystyrene nanoparticle with transferrin corona. The number of available epitopes describes how active the Nanoparticle will be in the body. For this purpose Single-cycle kinetic methodology was successfully used on nanoparticles. Single-cycle kinetic methodology has great potential to become the standard method for high throughput nanoparticle epitope characterization.

No description available.

Segmentation of the brain into sub-volumes has many clinical applications. Manyneurological diseases are connected with brain atrophy (tissue loss). By dividingthe brain into smaller compartments, volume comparison between the compartmentscan be made, as well as monitoring local volume changes over time. Theformer is especially interesting for the left and right cerebral hemispheres, dueto their symmetric appearance. By using automatic segmentation, the time consumingstep of manually labelling the brain is removed, allowing for larger scaleresearch.In this thesis, three automatic methods for segmenting the brain from magneticresonance (MR) images are implemented and evaluated. Since neither ofthe evaluated methods resulted in sufficiently good segmentations to be clinicallyrelevant, a novel segmentation method, called SB-GC (shape bottleneck detectionincorporated in graph cuts), is also presented. SB-GC utilizes quantitative MRIdata as input data, together with shape bottleneck detection and graph cuts tosegment the brain into the left and right cerebral hemispheres, the cerebellumand the brain stem. SB-GC shows promises of highly accurate and repeatable resultsfor both healthy, adult brains and more challenging cases such as childrenand brains containing pathologies.

Purpose: To model the time evolution of active caspase-3 protein expression in a healthy lens, and in a lens exposed to UVR-300 nm (UVR-B). To develop an automated method to classify the fluorescent signal of biomarkers in the lens epithelial cells. Methods: Six-week old Sprague-Dawley rats were used. Firstly, expression of active caspase-3 was studied in the lens epithelium of healthy rats. Secondly, rats were unilaterally exposed in vivo to 1 kJ/m2 UVR-B for 15 minutes. At 0.5, 8, 16, and 24 hours after the UVR-B exposure, the exposed and the contralateral non-exposed lenses were removed. Immunohistochemistry was done on three mid-sagittal sections from each lens. The florescent labelling for active caspase-3 in each lens section was counted three times. The time evolution of active caspase-3 expression in response to UVR-B exposure was modelled as a function of cell position in the lens epithelium. An automated objective method was developed to quantify the lens epithelial cells and to classify the fluorescent signal of active caspase-3. Active caspase-3 was selected as a model signal. Results: Active caspase-3 was abundant in the anterior pole of the normal lenses. Spatial distribution of active caspase-3 labelling in the lens epithelium was fitted to a logistic model. The probability of active caspase-3 expression was higher in the UVR-B exposed lenses (95% CI = 0.12 ± 0.01). There was no difference in the expression of active caspase-3 between the 0.5 and the 24 hours groups or between the 8 and the 16 hours groups. A difference was noted, when comparing the 0.5 and 24 hours groups with the 8 and 16 hours groups (Test statistic 7.01, F1;36;0.95= 4.11). Exposure to UVR-B has an impact on the average probability of labelling for active caspase-3 as a function of cell position. The probability of labelling as a function of cell number also varied as a function of time after UVR-B exposure. The automated method counted the lens epithelial cells and estimated the proportion of active caspase-3 labelling in the lens epithelium. Conclusions: Active caspase-3 is present in the healthy lens epithelial cells. Active caspase-3 exhibits higher expression at the anterior pole of the lens and the expression decreases towards the periphery. After UVR-B exposure, the expression of active caspase-3 in the lens epithelium increases with a peak of expression occurring around 16 hours after exposure. The average probability of labelling in the lens epithelium is dependent on both the UVR-B exposure and the time period elapsed after the exposure. The automated method enables objective and fast quantification of lens epithelial cells and the expression of fluorescent signal in the lens cells.

The intestinal tract of salmonids provides a dynamic interface that not only mediates nutrient uptake but also functions as the first line of defence against ingested pathogens. Exposure of the immune system to beneficial microorganisms and different dietary immunostimulants via the intestine has been shown to prime the immune system and help in the development of immune competence. Furthermore, the morphology and function of teleostean intestines are known to respond to feed components and to ingested and resident bacterial communities. Histological appraisal is still generally considered to be the gold standard for sensitive assessment of the effects of such dietary modulation. The aim of the present study was to improve understanding of salmonid intestinal function, structure and dynamics and to use the knowledge gained to develop a model for analysis, which would allow intestinal health to be assessed with respect to different intestinal communities and feed components. Virtual histology, the process of assessing digital images of histological slides, is gaining momentum as an approach to supplement traditional histological evaluation methodologies and at the same time, image analysis of digitised histological sections provides a practical means for quantifiable assessment of structural and functional changes in tissues, being both objective and reproducible. This project focused on the development of a rapid, practical analytical methodology based on advanced image analysis, that was able to measure and characterise a range of features of the intestinal histology of Atlantic salmon in a quantitative manner. In the first research chapter, the development of a novel histological assessment system based upon advanced image analysis was described, this being developed with the help of a soybean feed model known to induce enteropathy in Atlantic salmon. This tool targeted the evaluation of the extent of morphological changes occurring in the distal intestine of Atlantic salmon following dietary modulation. The final analytical methodology arrived at, could be conducted with minimal user-interaction, allowing rapid and objective assessment of 12 continuous variables per histological frame analysed. The processing time required for each histological frame was roughly 20-25 min, which greatly improved the efficiency of conducting such a quantitative assessment with respect to the time taken for a subjective semi-quantitative alternative approach. Significant agreement between the fully automated and the manual morphometric image segmentation was achieved, however, the strength of this quantitative approach was enhanced by the employment of interactive procedures, which enabled the operator / observer to rectify preceding automated segmentation steps, and account for the specimen’s variations. Results indicated that image analysis provided a viable alternative to a pathologist’s manual scoring, being more practical and time-efficient. In the second research chapter, feeding Atlantic salmon a high inclusion level of unrefined SBM (25 %) produced an inflammatory response in the distal intestine as previously described by other authors. The model feed trial successfully generated differentiable states, although these were not, for the most part, systemically differentiable through the majority of standard immunological procedures used, being only detectable morphologically. Quantitation of morphometric parameters associated with histological sections using the newly developed image analysis tool successfully allowed identification of major morphological changes. Image analysis was thus shown to provide a powerful tool for describing the histomorphological structure of Atlantic salmon distal intestine. In turn, the semi-automated image analysis methods were able to distinguish normal intestinal mucosa from those affected by enteritis. While individual parameters were less discriminatory, use of multivariate techniques allowed better discrimination of states and is likely to prove the most productive approach in further studies. Work described in the third research chapter sought to validate the semi-automated image analysis system to establish that it was measuring the parameters it was purported to be measuring, and to provide reassurance that it could reliably measure pre-determined features. This study, using the same sections for semi-quantitative and quantitative analyses, demonstrated that the quantitative indices performed well when compared to analogous semi-quantitative descriptive parameters of assessment for enteritis prognosis. The excellent reproducibility and accuracy performance levels indicated that the image analysis system was a useful and reliable morphometric method for the quantification of SB-induced enteritis in salmon. Other characteristics such as rapidity, simplicity and adaptability favour this method for image analysis, and are particularly useful where less experienced interpreters are performing the analysis. The work described in the fourth research chapter characterised changes in the morphology of the intestinal epithelial cells occurring as a result of dietary modulation and aspects of inflammatory infiltration, using a selected panel of enzyme and IHC markers. To accomplish this, image analysis techniques were used to evaluate and systematically optimise a quantitative immunolabelling assessment protocol. Digital computer-assisted quantification of labelling for cell proliferation and regeneration; programmed cell death or apoptosis; EGCs and t-cell like infiltrates; mobilisation of stress-related protein regenerative processes and facilitation of nutrient uptake and ion transport provided encouraging results. Through the description of the intestinal cellular responses at a molecular level, such IHC expression profiling further characterised the inflammatory reaction generated by the enteropathic diet. In addition, a number of potential diagnostic parameters were described for fish intestinal health e.g. the relative levels of antigenicity and the spatial distribution of antigens in tissues. Work described in the final research chapter focused on detailed characterisation of intestinal MCs / EGCs in order to try to elucidate their functional role in the intestinal immune responses. Through an understanding of their distribution, composition and ultrastructure, the intention was to better characterise these cells and their functional properties. The general morphology, histochemical characteristics and tissue distribution of these cells were explored in detail using histochemical, IHC and immunogold staining / labelling, visualised using light, confocal and TEM microscopy. Despite these extensive investigations, their physiological function and the content of their granules still remain somewhat obscure, although a role as immunodulatory cells reacting to various exogeneous signals through a finely regulated process and comparable to that causing the degranulation of mammalian MCs is suggested. The histochemical staining properties demonstrated for salmonid MCs / EGCs seem to resemble those of mammalian mucosal mast cells, with both acidophilic and basophilic components in their granules, and a granule content containing neuromodulator / neurotransmitter-peptides such as serotonin, met-enkephalin and substance-p. Consequently, distinguishable bio-chromogenic markers have been identified that are of utility in generating a discriminatory profile for image analysis of such cells.

This master’s thesis has the intent of showing that the tattoo can be regarded as an individuals document according to prevailing notions in archival science. Suzanne Briets definition of the concept of document is used in this thesis as a widening definition, a document does not have to presented in a defined form, it is only required to represent an object or intellectual phenomenon and to be informative. Tattoos are presented in this thesis as informative with regard to the status and position of russian/soviet convicts and as such presenting an individuals identity within a specific context. As documents they also provide the individual with the essential evidence of his or her endeavours in a criminal environment. Also, they have the ability to function as an individuals memories of relationships, hardships and comradeships. Memory, evidence and identity are concepts upon which archival theorists are reworking the role of archives and documents in society and culture. The method presented in this thesis is image analysis applied to a selected number of photographs and drawings owned and published by a design and publishing company in London. The image analysis has as its focus the tattoos visible upon individuals bodies, these tattoos are interpreted and made relevant with regard to historical circumstances and the prison environment. The image analysis in this thesis is complemented by litterature studies. The thesis also provides a recapitulation regarding previous research concerning tattoos within the human and social studies. The conclusion this thesis presents is that it is possible to view the tattoo as a document, bound to an individual, which mirrors the society and culture in which the individual finds himself. It is also possible to view the tattoo as mirroring indivudals possibilities, wishes and memories. Finally, the tattoo presents itself, in this context, as a document that may represent a critique of a dominant society or simply the voice of the alienated.

Clinical immunohistochemistry (IHC) techniques are not yet fully standardized. In this project, a standardization method was developed and tested for proficiency testing (PT) in external quality assurance (EQA) and quality control (QC) in clinical IHC laboratories. The breast cancer markers estrogen receptor (ER), progesterone receptor (PR) and human epidermal growth factor 2 (HER2) were used as a model system. Digital image analysis (IA) was used in conjunction with new calibrated and standardized cell line microarrays (CLMA). CLMAs built from nine formalin-fixed paraffin-embedded (FFPE) breast cancer cell lines were used for both QC controls and PT samples, instead of traditionally used FFPE tissues, in the standardization of breast cancer IHC. IA was used for measurement of IHC results, and compared to evaluation by the traditional expert-assessment method. Laboratory Score: Reference Score Ratio (LSRSR) was derived from Histo-Scores (HScores) determined by IA. HScores and LSRSRs were examined statistically and evaluated as histograms and boxplots to summarize and rank participant laboratory EQA results, in comparison to a reference sample or reference laboratories in two consecutive Canada-wide EQA runs. LSRSR-derived reference ranges were highly sensitive in evaluating laboratory EQA performance in PT as well as for monitoring of controls for QC. Laboratory on-slide tissue and cell-line IHC QA controls were assessed using IA and Levey Jennings QC charts. These charts were determined to be an excellent way to observe trending in laboratory IHC staining over time, particularly when cell line controls were used. This approach also reduced the time and labor costs for PT evaluation. Overall, cell line calibration controls were functionally equivalent or better than tissue-based controls in QC and PT mainly because of cell line biological homogeneity and sample availability. This study identified an optimal design for preparation of IHC cell line controls and PT samples for breast cancer markers. Optimal, intermediate staining cell line IHC controls were identified for all three breast cancer markers. Using IA with LSRSR and cell line samples is recommended for standardization of IHC methodology. This approach advances QA for diagnostic IHC and when implemented will improve patient care

Composite materials are usually multi-phase materials, made up from two or more phases, which are combined to provide properties that the individual constituents cannot. This technology represents an economical way to improve product performances avoiding the use of expensive materials. Composite materials can be obtained as films by means of the electrolysis of electroplating solutions in which micrometre- or submicrometre-size particles are suspended: variable amounts of these particles become incorporated in the electrochemically produced solid phase, to which they impart enhanced properties. The main aims of the present work contributing to this thesis are the study of different parameters influencing the electroco-deposition process in order to promote and improve the applicability of such a technology in the high speed electroplating industry. Following a comprehensive review on the electroco-deposition of composite coatings, the phenomena have been analysed moving from a microscopic point of view i. e. the role of the metal ions present in the electrolyte and adsorption on the inert particles and their interactions with the growing metal layer, to a macroscopic point of view i. e. the electrolyte agitation, its influence on particle motion and all the issues related to the presence of particles in an electrolyte during electroplating. In particular the inert particle influence in terms of geometry, dimension and chemical nature (spherical polystyrene particles vs. irregular alumina particles with different dimensions), the metal matrix influence (nickel, copper and zinc), the influence of electrolyte agitation (using a Rotating Cylinder Electrode cell system) and the influence of the coating thickness on particle content in the final coating, using different deposition times, have been examined. The importance of the particle shape has been highlighted showing how incorporating irregular geometries gave higher particle incorporation densities than regular geometries. The influence of the substrate finishing in terms of imperfections has been related to the particle incorporation rate showing how small surface imperfections enhanced the incorporation of particles. Different hydrodynamic regimes have been analysed resulting three different regimes being discerned: laminar, transitional and turbulent. The consequence, in terms of particle incorporation levels, has been found showing how the amount of particles in the coating changed from one regime to another. Different rate-determining steps were related to the hydrodynamics: when the regime is laminar, particles were incorporated as agglomerates and the process was under particle transfer control, whilst in the turbulent zone, the rate determining step was the velocity of reduction of the ions adsorbed on the particle surface.