Current therapy for chronic kidney disease (CKD) is limited; however, recent studies have shown that a subpopulation of cells derived from the bone marrow, known as early outgrowth cells (EOCs), are able to attenuate kidney injury. Here we examined the efficacy of a modular tissue engineering system whereby the EOCs might be easily removed in the event of malignant change. While modular therapy mimicked the effects seen with standard EOC therapy, the modules degraded allowing the encapsulated EOCs to enter systemic circulation. Given the presumed egress of EOCs, we explored an alternative strategy for kidney protection. Here we investigated the long-term effectiveness of administering the conditioned medium (EOC-CM) that contains the factors the EOCs secrete, rather than the cells themselves. In these studies, repeated administration of EOC-CM attenuated the structural and functional manifestations of kidney injury suggesting that this approach may provide an effective and feasible, cell-free approach for CKD.

Nuolat kintanti aplinka yra pagrindinis veiksnys, kontroliuojantis mikroorganizmų augimą ir vystymąsi. Evoliucijos eigoje organizmuose išsivystė signalinės sistemos, gebančios sujungti aplinkos signalus su ląstelės transkripcijos, transliacijos ir kt. procesais. Viena iš tokių universalių signalinių sistemų yra Ras/PKA signalinis kelias. Ši sistema leidžia mielių ląstelėms reaguoti į aplinkoje esančius maisto medžiagų šaltinius ir įvairius stresinius veiksnius. Vienas iš pagrindinių aplinkos signalų, įtakojančių ląstelių fiziologiją, yra aplinkos pH. Mielių ląstelėse į aplinkos pH reaguoja Rim101 signalinė sistema, kuri kartu su Ras/PKA reguliuoja tokius procesus kaip sporuliacija ir pseudohifinis augimas. Ekstraląstelinės terpės rūgštėjimas lemia viduląstelinį rūgštėjimą, o tai sukelia mielių ląstelėms stresą ir taip aktyvina Ras/PKA signalinį kelią. Streso poveikio pasekmė yra ląstelių augimo sulėtėjimas, gyvenimo trukmės trumpėjimas, augimo sustabdymas ar netgi žūtis. Pastaraisiais metais parodyta, kad būtent Ras/PKA signalinis kelias reguliuoja ląstelės senėjimo procesą kaip atsaką į aplinkos pH. Taigi mielių, kaip modelinio organizmo, panaudojimas žmogaus genų, reguliuojančių ląstelės senėjimą ir žūtį, tyrimuose vis plečiasi. Disertacinio darbo metu buvo įvertintas izogeninių mielių kamienų turinčių Ras/PKA signalinio kelio komponentų mutacijas augimas bei mitybinės terpės pH kitimas. Nustatyta, kad mutacijos Ras/PKA signalinio kelio komponentuose lemia skirtingą... [toliau žr. visą tekstą] / Constantly changing environment is the major factor controlling the growth and development of the microorganisms. For quick generation of the cell response, information about changes in the cell environment is rapidly transmitted to the inner molecules of the cell. During the course of evolution the cells have developed signaling systems that are able to combine extracellular signals with the inner processes, as transcription, translation etc. One of the universal signaling systems is Ras/PKA signal transduction pathway. This system helps cells recognize the nutrient sources present in the growth medium.Environmental pH is one of the main factors influencing the growth, physiology and differentiation of yeast. In Saccharomyces cerevisiae, response to pH is determined by the RIM101 pathway. Recently, it was shown, that Ras/PKA signal transduction pathway regulates cell aging as response to environmental pH. Many aging and apoptosis features are conserved between yeast and multicellular microorganisms, and this makes them perfect model organisms. Yeasts are also suitable also for acidosis related disease studies. Evaluation of the cell growth and medium acidification of isogenic strains containing mutation in the members of Ras/PKA signal transduction pathway was performed. Mutations in these genes cause changes in metabolic activity of the cell.Members of the Ras/PKA signal transduction pathway participate in regulation of cell viability and lifespan during the natural... [to full text]

Moving groups are associations of stars which originated from the same star forming region. These groups are typically young (< 200 Myr) since they have not dissipated into the galactic field population. Over the last 15 years, roughly 10 such moving groups have been found with distances < 150 pc (7 with distances < 100 pc), each with a unique velocity and position. This work first investigates the likelihood to resolve star from two moving groups (AB Doradus and Beta Pictoris) using high spacial resolution optical interferrometry and found 5 AB Doradus stars and 1 Beta Pictoris star with declinations > -30 could be spacially resolved. To more deeply characterize individual groups, we used the 2.7m telescope at the McDonald Observatory to observe 10 proposed AB Doradus stars and 5 proposed Octans-Near stars (3 probable members, 2 possible) with high resolution (R ~60,000) optical spectroscopy. Each group is characterized in three ways: (1) Chemical analysis to determine the homogeneity among members, (2) Kinematic traceback to determine the origin, and (3) Isochrone fitting to determine the age. We find the 8 stars in our AB Doradus sample are chemically homogeneous with [M/H] = -0.03 ± 0.06 dex, traceback to an age of 125 Myr, and the stars in this mass range are on the main sequence. The two deviants are a metal rich, potentially younger member and a metal poor, young star likely not associated with AB Doradus. In our Octans-Near sample, we find the 3 probable members have [M/H] = -0.06 ± 0.11, the stars do not trace back to a common origin, and the probable members are on the main sequence. In addition to these tests, we found that the probable members are slightly more lithium depleted than the Pleiades, implying an age between 125 and 200 Myr. Finally, we investigate systematic trends in fundamental stellar parameters from the use of different techniques. Preliminary results find differences in temperatures between interferrometric and spectroscopic techniques to be a function of temperature with a interferrometric temperatures being cooler by an average of 36 ± 115 K. We also calculated the chemical abundances as a function of condensation temperature for our moving group sample and predict 2 stars in AB Doradus could represent the initial star forming environment and discuss the implications for planet hosting stars in nearby moving groups. This updated characterization technique allows for a deeper understanding of the moving group environment. As future, high precision instruments emerge in astronomy (Jame Webb Space Telescope, GAIA, 30m class telescopes), moving groups are ideal targets since these associations will help us understand star forming regions, stellar evolution at young ages, constrain stellar evolutionary models, and identify planetary formation and evolution mechanisms.

The construction of the James Webb Space Telescope has brought attention to infrared astronomy and cosmology. The potential information about our universe to be gained by this mission and future infrared telescopes is staggering, but infrared observation faces many obstacles. These telescopes face large amounts of noise by many phenomena, from emission off of the mirrors to the cosmic infrared background. Infrared telescopes need to be designed in such a way that noise is minimized to achieve sufficient signal to noise ratio on high redshift objects. We will investigate current and planned space and ground based telescopes, model the noise they encounter, and discover their limitations. The ultimate goal of our investigation is to compare the sensitivity of these missions in the near and mid IR and to propose new missions. Our investigation is broken down into four major sections: current missions, noise, signal, and proposed missions. In the proposed missions section we investigate historical and current infrared telescopes with attention given to their location and properties. The noise section discusses the noise that an infrared telescope will encounter and set the background limit. The signal section will look at the spectral energy distributions (SED) of a few significant objects in our universe. We will calculate the intensity of the objects at various points on Earth and in orbit. In the final section we use our findings in the signal and noise sections to model integration times (observation time) for a variety of missions to achieve a given signal to noise ratio (SNR).

A two dimensional hydrodynamic model describing the solar wind interaction with the local interstellar medium, which surrounds the solar system, is used to study the heliosphere both as a steady-state- and dynamic structure. The finite volume method used to solve the associated system of hydrodynamic equations numerically is discussed in detail. Subsequently the steady state heliosphere is studied for both the case where the solar wind and the interstellar medium are assumed to consist of protons only, as well as the case where the neutral hydrogen population in the interstellar medium is taken into account. It is shown that the heliosphere forms as three waves, propagating away from the initial point of contact between the solar wind and interstellar matter, become stationary. Two of these waves become stationary at sonic points, forming the termination shock and bow shock respectively. The third wave becomes stationary as a contact discontinuity, called the heliopause. It is shown that the position and geometry of the termination shock, heliopause and bow shock as well as the plasma flow characteristics of the heliosphere largely depend on the dynamic pressure of either the solar wind or interstellar matter. The heliosphere is modelled as a dynamic structure, including both the effects of the solar cycle and short term variations in the solar wind observed by a range of spacecraft over the past ~ 30 years. The dynamic model allows the calculation of an accurate record of the heliosphere state over the past ~ 30 years. This record is used to predict the time at which the Voyager 2 spacecraft will cross the termination shock. Voyager 1 observations of 10 MeV cosmic ray electrons are then used in conjunction with a cosmic ray modulation model to constrain the record of the heliosphere further. It is shown that the dynamic hydrodynamic model describes the heliosphere accurately within a margin of error of ±0.7 years and ±3 AU. The model predicts that Voyager 2 crossed the termination shock in 2007, corresponding to preliminary results from observations indicating that the crossing occurred in August 2007. / Thesis (M.Sc. (Physics))--North-West University, Potchefstroom Campus, 2008.

This document describes studies of two independent regions of the interstellar medium (ISM). These studies have the common element that both pertain to regions in our Galaxy that are known to be associated with present-day star formation. These studies aim to help us understand the ISM, star formation, and ultimately where we came from, since, after all, our star, the Sun, is itself the product of star formation 4.5 billion years ago. The first project measured the Zeeman Effect on the 21 cm H I absorption line in order to create a map of the line of sight magnetic fields near a star forming region called W3. From the map of the field, it was possible to create a three dimensional model of the magnetic field morphology. Also, calculating the various energies associated with W3 revealed that it is most likely in virial equilibrium, not expanding or contracting. The second project used an instrument on the Hubble Space Telescope (HST) to measure the abundance of iron in a neutral region near the Orion Nebula called Orion’s Veil. One of the goals of this project is to investigate whether solid dust grains can be destroyed by ionizing radiation by comparing the amount of solid iron in Orion’s Veil to the amount in the nearby ionized regions. By measuring the depletion of iron in the neutral Veil and comparing it to the depletion of iron the H+ regions of the Orion Nebula, it was possible to conclude that iron was not being released into the gas phase by ultraviolet photons. In addition, oscillator strengths for two Fe II transitions were measured.

This thesis aimed to investigate the impact of firm- and industry-specific factors on the decision to adopt Internet technologies among SMEs in New Zealand's retail and tourism industries. A mail survey was sent to 500 retail SMEs and 1,000 tourism SMEs, with response rates of 26.4 percent and 33.4 percent respectively. The results indicated that, in a comparison of perceived benefits, organizational readiness and external pressure, the most important factor in determining the adoption decision was the perceived benefits. Overall, the level of Internet support, business size, international business, and years on the Internet were the most important structural factors in determining Internet technology adoption. When comparing retail and tourism SMEs, the level of Internet support within a firm, business size, and years on the Internet were more important factors for the tourism SMEs than for their retail counterparts. On the other hand, business type and international business were more important factors for retail SMEs than they were for tourism firms. The research also found that tourism SMEs experienced greater pressure from the external environment to adopt Internet technologies than did retail firms.

This study examines university English-medium Instruction (EMI) reform implementation approaches from a comparative organizational perspective. Over the last decade, the number of master’s degree programs instructed exclusively in English in non-Anglophone Europe increased dramatically. Europe is an interesting case as it actively promotes multilingual learning; however, many European policies over the last twenty years accelerated the rise of monolingual EMI reforms, especially at the graduate-level. The purpose of this exploratory study is to contribute to our understanding of how widespread EMI reforms impact structures and behaviors at the organizational level in European universities in ways that respond to the organization’s embedded policy contexts. This research aims to advance our understandings of comparative EMI reforms and also, drawing on the concepts of neoinstitutional theory, develop our knowledge of how these processes might be theorized and expanded. I combine the theoretical frames of translation and institutional logics to analyze empirical case studies of the implementation of the EMI reform concept in three Northern European universities in leading EMI provider countries: the University of Oslo in Norway, the University of Göttingen in Germany, and Maastricht University in the Netherlands. The theoretical concept of institutional complexity is used to analyze the contending tensions universities confront when deciding the best way to design and implement EMI reforms. The three-axis comparative framework developed in this study represents a novel approach to examining variations in EMI reform implementation. Variations in organizational EMI implementation approaches (collegial, targeted, and market) are understood by analyzing comparatively how the three universities interpreted axial tensions between institutional logics for the best way to organize their EMI reform approaches: for academic or economic purposes; cooperative or competitive purposes; and local or global purposes. This comparative case study underscores the importance of examining a university’s embedded environment (both European and local levels) to understand university response to widespread EMI reform trends and highlights the significance of contextual dynamics to European EMI program development policy. The study concludes with policy recommendations and future directions.

Existing research shows that plants produce less root when growing alone than when growing in competition with other plants. When plants under root competition over-allocate resources to roots at the cost of reproduction, it represents a Tragedy of the Commons. I constructed simulation models to determine the circumstances likely to give rise to a Tragedy of the Commons, and explore mechanisms by which plants may solve it. I grew plants in nutrient-rich transparent gel, allowing me to quantify root growth and development without destructive sampling. My plants responded positively to additional space and the presence of a competitor at full nutrient treatment levels, and negatively to those same conditions between low phosphorus treatment levels, demonstrating nutrient mediation of the direction of plant response to an added competitor with additional space. This effect may feature self / non-self recognition by roots. Since the hard barrier in these studies blocks nutrients, roots, and root signaling compounds from passing between the plants in the barrier treatment level, existing studies cannot tease apart the effects on plant development of these individual factors. I add a semi-permeable membrane treatment level, which allows nutrients and signaling compounds to pass while preventing root growth between sides.

RR Lyrae stars are old, iron-poor, Helium-burning variable stars. RR Lyraes are extremely useful for tracing phase-space structures and metallicities within the galaxy because they are easy to identify, have consistent luminosities, and are found in large numbers in the galactic disk, bulge, and halo. Here we present a new set of spectroscopic metallicity calibrations that use the equivalent widths of the Ca II K, Hγ, and Hδ lines to calculate metallicity values. Applied to spectroscopic survey data, these calibrations will help shed light on the evolution of the Milky Way and other galaxies.