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Schifffahrt stellt weltweit eine der wichtigsten Nutzungen der Flüsse und Seen dar, die zukünftig weiter zu nimmt. Sie schädigt Ufer durch Wellenschlag erheblich. Die Effekte von Schiffswellen auf benthische Wirbellose sind aber bisher kaum bekannt, obwohl diese eine zentrale Rolle im litoralen Nahrungsnetz spielen. Daher untersuchte ich 1) die direkten Effekte von Schiffswellen auf benthische Wirbellose, 2) die resultierenden Auswirkungen auf trophische Interaktionen und 3) das Wachstum und die Fitness von Wirbellosen, sowie 4) die langfristigen Änderungen der litoralen Wirbellosenzönosen. Labor- und Feldversuche zeigten, dass mit zunehmender wellengenerierter Sohlschubspannung mehr Individuen verdriftet wurden. Die Verdriftung wurde jedoch durch eine hohe strukturelle Habitatkomplexität gemindert, da diese die Wellenenergie stärker dissipierte und den Wirbellosen bessere Festhaltemöglichkeiten bot. Die Verdriftung der Wirbellosen bewirkte ein höheres Prädationsrisiko durch spindelförmige Fische, während hochrückige Fische die Ingestion bei Wellenexposition reduzierten. Wellenschlag verringerte auch das Wachstum und die Fitness nativer Wirbellosenarten, indem die Ingestion verringert oder der Energieverbrauch erhöht wurde, wohingegen Neozoen nicht beeinträchtigt wurden. Der kumulative Effekt von Schiffswellen veränderte die Artenzusammensetzung benthischer Zönosen sehr. Die Abundanz nativer Wirbelloser und die Artenzahl waren an exponierten Ufern geringer, während die Abundanz invasiver Arten zunahm. Folglich beeinträchtigen Schiffswellen benthische Wirbellose auf der Ebene der Individuen, Arten, Zönosen, sowie tropischer Interaktionen, und können so die ökologische Struktur und Funktion des gesamten Litorals beeinflussen. Durch Schutz komplexer Habitate wie Wurzeln und dichte Schilfbestände, sowie durch Wellenschlagsreduzierung durch größere Mindestabstände zum Ufer und angepasste Fahrtgeschwindigkeit, können die Auswirkungen von Schiffswellen gemindert werden. / Inland navigation is a major human use of rivers and lakes worldwide which is expected to increase in the future. It significantly affects shore habitats by ship-induced waves. In contrast to the importance of such pressures, the effects of these hydrodynamic disturbances on benthic invertebrates in the littoral zones are poorly understood, even that invertebrates are a central element of littoral food webs. Hence, I investigated 1) the direct and immediate effects of ship-waves on benthic invertebrates, 2) their subsequent effects on trophic interactions and 3) on the growth and fitness of invertebrates, and finally 4) the long-term effects on the community composition. Laboratory and field experiments showed increasing detachment of invertebrates with higher wave-induced shear stress. Detachment was significantly mitigated by higher structural complexity of the habitats, as complex habitats dissipate wave energy and provide better fixing possibilities. Moreover dislodgement of invertebrates led to a higher risk of being preyed upon by fusiform fish, while deep bodied fish reduced feeding under waves. Waves also reduced growth and fitness of native invertebrates via reduced feeding or increased energy costs, while non-native invertebrates were not affected. The cumulative impact of ship-waves alters the community composition of benthic invertebrates. The abundance of native invertebrates and total species richness was lower at exposed sites, while non-native invertebrates increased in abundance. Thus, ship-waves affect benthic invertebrates on the individual, species, and community levels, as well as the interaction of trophic levels, and hence will alter the ecological structure and function of whole littoral zones. Adverse effects of ship-waves may be mitigated by protecting structural complex habitats such as tree roots and dense reed belts, and by minimizing wave generation by increasing minimum sailing distance to shore or by adjusting vessel speed.

The KwaZulu-Natal Drakensberg, South Africa, is predominantly a grassland ecosystem maintained by fire. The effect of the current burning regime on invertebrate and flowering forb diversity in this ecosystem is poorly understood. The overall aim ofthis study was to contribute towards the development of an effective burning regime for the KwaZulu-Natal Drakensberg that will conserve invertebrates and indigenous forbs, two major components of biodiversity. The objectives were to examine the effect of fire and fire frequency on flowering forb and invertebrate species diversity, to determine whether fire frequency, time since last burn or locality were influencing species composition, and to identify potential biodiversity indicators that reflect overall species richness for use in monitoring of invertebrates and forbs. Sampling took place in March, September and November of 2002 at Giants Castle Game Reserve. Invertebrates were sampled using sweep netting and targeted netting along transects, yellow pan traps and soil quadrats. Invertebrate taxa sampled were ants (Formicidae), butterflies (Lepidoptera), grasshoppers (Orthoptera), leafboppers (Cicadellidae), bees (Apoidea), bee flies (Bombyliidae), hover flies (Syrphidae), robber flies (Asilidae), spiders (Araneae), earthworms (Oligochaeta) and millipedes (Diploda). These were identified to species level with the assistance of taxon experts. Flowering forbs were sampled using five replicates of five by five metre quadrats randomly placed in each site. Overall flowering forb and invertebrate species diversity was higher in grasslands that were burnt for two consecutive years in 2001 and 2002 than in grasslands that were not burnt during those two years. Frequently (annual) and intermediately (biennial) burnt grasslands had significantly higher invertebrate and flowering forb diversity than infrequently (five years without burning) burnt grasslands. This, together with the fact that grasslands burnt during the year of sampling had higher species richness than grasslands burnt two and five years previously suggests that invertebrates and forbs are generally resilient to fire and many forb species appear to be stimulated by fire. However, each burn frequency had its own suite of unique flowering forb and invertebrate species. Invertebrate communities were influenced mostly by locality and the length of time past since the last fire and flowering forb communities were influenced mostly by the length oftime past since the last fire. Fire frequency had the least influence on both invertebrate and forb communities. Ecological succession occurred after each fire in the invertebrate communities but forb communities appear to need more than five years without fire for ecological succession to occur. The findings of this study therefore suggest that using a combination of three fire frequencies would result in patches of grassland in various stages of ecological succession, and would conserve species unique to each burning frequency, and would therefore conserve maximum diversity. Flowering forb species richness and certain invertebrate taxa (ants, leafboppers, spiders and bees) have the potential to act as indicators of overall invertebrate species richness for use in monitoring programmes. / Thesis (M.Sc.)-University of KwaZulu-Natal, Pietermaritzburg, 2006.

Physical and chemical influences downstream of surface coal mines, including selenium (Se) release, water quality shifts, and habitat alterations can affect aquatic organisms. To evaluate these influences at the community level of organization, fish and macroinvertebrates were studied in mine-affected and reference streams. Se can be toxic to aquatic organisms and was measured in lotic food chains (water, biofilm, macroinvertebrates and juvenile salmonids). Invertebrate Se was significantly related to Se in juvenile fish muscle (westslope cutthroat, bull, rainbow and brook trout) and Se concentrations exceeded proposed individual-level reproductive effects thresholds in some rainbow and cutthroat trout. Community-level effects were only detected in rainbow trout where species specific biomass was negatively related to muscle Se concentration in stream reaches. Macroinvertebrate assemblages varied along a mineinfluence gradient defined by Se, alkalinity, substrate embeddedness and interstitial material size. Ephemeroptera were the most sensitive to mining effects and potential mechanisms influencing community composition included Se and ion toxicity and habitat degradation. This project highlights the need to study multiple organisms at different levels of ecological organization in order to understand and manage diverse mining impacts. / xi, 108 leaves : col. maps ; 29 cm

MSc (Zoology) / Department of Zoology / See the attached abstract below

The European Water Framework Directive 2000 established a new emphasis for the management of freshwaters by setting ecologically-based water quality targets that are to be achieved through holistic, catchment-scale, ecosystem management. However, significant knowledge gaps exist in the understanding of the cumulative effectiveness of multiple mitigation measures on a number of pollutants at a catchment scale. This research contributes to improved understanding of the effectiveness of an ecosystem management approach to deliver catchment-scale water quality improvements on the National Trust Holnicote Estate on Exmoor, UK. This research is part of a larger multi-objective project funded by the UK Department for Environment, Food and Rural Affairs (Defra), to demonstrate the benefits of land use interventions for the management of flood risk. This thesis evaluates the effects of upland ditch blocking on physico-chemical and biological parameters of water quality in an upland Horner Water catchment one year after habitat restoration, and establishes a solid baseline for the monitoring of the effects of current and future land management changes in a lowland, intensively managed, agricultural Aller catchment. The spatial variability of soil physical and chemical properties (bulk density, total carbon (TN), nitrogen (TN), C:N ratio, δ15N, total phosphorus (TP), inorganic phosphorus (IP), organic phosphorus (OP)) and water quality determinands (suspended sediment (SS), dissolved organic carbon (DOC), total particulate carbon (TPC), total oxidised nitrogen (TON) and dissolved reactive phosphorus (DRP)) in the two study catchments with contrasting land use has been characterised and linked to the prevailing land use. Agricultural land use resulted in extensive homogenisation of soil properties. The spatial dependence of all soil properties, except for bulk density and δ15N, was stronger in the agricultural than the semi-natural catchment (nugget:sill ratio 0.10-0.42 in the Aller and 0.15-0.94 in Horner Water), while bulk density, TP, inorganic phosphorus (IP), organic phosphorus (OP), C:N ratio, δ15N and carbon storage showed a longer range of spatial auto-correlation in the agricultural catchment (2,807-3,191 m in the Aller and 545-2,599 m Horner Water). The central tendency (mean, median) of all soil properties, except for IP and δ15N, also differed significantly between the two catchments (P < 0.01). The observed extensive alteration of soil physical and chemical properties in the agricultural catchment is likely to have long-term implications for the restoration of ecosystem functioning and water quality management. The intensive land use seems to have resulted in an altered ‘catchment metabolism’, manifested in a proportionally greater total fluvial carbon (dissolved and particulate) export from the agricultural than the semi-natural catchment. The agricultural catchment supported significantly higher DOC concentrations (P < 0.05) and the quality of DOC differed markedly between the two study catchments. The prevalence of more humic, higher molecular weight compounds in the agricultural catchment and simpler, lower molecular weight compounds in the semi-natural catchment, indicated enhanced microbial turnover of fluvial DOC in the agricultural catchment as well as additional allochtonous terrestrial sources. During an eight month period for which a comparable continuous turbidity record was available, the estimated SS yields from the agricultural catchment (25.5-116.2 t km2) were higher than from the semi-natural catchment (21.7-57.8 t km2). Further, the agricultural catchment exported proportionally more TPC (0.51-2.59 kg mm-1) than the semi-natural catchment (0.36-0.97 kg mm-1) and a similar amount of DOC (0.26-0.52 kg mm-1 in the Aller and 0.24-0.32 kg mm-1 in Horner Water), when normalised by catchment area and total discharge, despite the lower total soil carbon pool, thus indicating an enhanced fluvial loss of sediment and carbon from the intensively managed catchment. Whilst detection of catchment-scale effects of mitigation measures typically requires high resolution, resource-intensive, long term data sets, this research has found that simple approaches can be effective in bridging the gap between fine scale ecosystem functioning and catchment-scale processes. Here, the new macro-invertebrate index PSI (Proportion of Sediment-sensitive Invertebrates) has been shown to be more closely related to a physical measure of sedimentation (% fine bed sediment cover) (P = 0.002) than existing non-pressure specific macro-invertebrate metrics such as the Lotic Index for Flow Evaluation (LIFE) and % Ephemeroptera, Plecoptera & Trichoptera abundance (% EPT) (P = 0.014). Further testing of PSI along a pronounced environmental gradient is recommended as PSI and % fine bed sediment cover have the potential to become a sensitive tool for the setting and monitoring of twin sedimentation targets. Upland ditch management has not had any discernible effect on water quality in the semi-natural upland catchment one year after restoration, which may be due to the short-term post-restoration monitoring period but may also reflect benign effects of large-scale earth moving works on this high quality environment. The conceptual understanding of catchment processes developed in this thesis suggests that cumulatively, the recently completed mitigation works in the lowland agricultural catchment will likely result in reduced sediment and nutrient input into the aquatic environment. However, further research is needed to build on this detailed baseline characterisation and inform the understanding of the effectiveness of combined mitigation measures to reduce the flux of multiple contaminants at the catchment scale.

The cardiovascular system of bilaterians developed from a common ancestor. However, no endothelial cells exist in invertebrates demonstrating that primitive cardiovascular tubes do not require this vertebrate-specific cell type in order to form. This raises the question of how cardiovascular tubes form in invertebrates? Here we discovered that in the invertebrate cephalochordate amphioxus, the basement membranes of endoderm and mesoderm line the lumen of the major vessels, namely aorta and heart. During amphioxus development a laminin-containing extracellular matrix (ECM) was found to fill the space between the basal cell surfaces of endoderm and mesoderm along their anterior-posterior (A-P) axes. Blood cells appear in this ECM-filled tubular space, coincident with the development of a vascular lumen. To get insight into the underlying cellular mechanism, we induced vessels in vitro with a cell polarity similar to the vessels of amphioxus. We show that basal cell surfaces can form a vascular lumen filled with ECM, and that phagocytotic blood cells can clear this luminal ECM to generate a patent vascular lumen. Therefore, our experiments suggest a mechanism of blood vessel formation via basal cell surfaces in amphioxus and possibly in other invertebrates that do not have any endothelial cells. In addition, a comparison between amphioxus and mouse shows that endothelial cells physically separate the basement membranes from the vascular lumen, suggesting that endothelial cells create cardiovascular tubes with a cell polarity of epithelial tubes in vertebrates and mammals.

Thesis (MSc)--University of Stellenbosch, 2005. / ENGLISH ABSTRACT: The primary aim of this study was to assess and compare the ecological integrity of the Lourens, Palmiet and Hout Bay Rivers (South Western Cape, South Africa) by examining the macroinvertebrate community structure at a series of representative sampling sites along the course of each river, using the South African Scoring System - Version 5 (SASS-5) rapid bioassessment method. Secondary aims included an examination of the effects of seasonal variability, biotope availability and site-specific environmental variables on the macroinvertebrate community structure at sampling sites, as well as the preliminary testing of the Integrated Habitat Assessment System (IHAS) for aquatic macroinvertebrates. According to results obtained, the ecological integrity of sampling sites in the Mountain Stream Zone of the three rivers was consistently good. The Hout Bay River in the upper portions of the Orange Kloof Reserve was particularly near-pristine, with this area having been identified in this study as a potential biodiversity ‘hot-spot’ for aquatic macroinvertebrates. Downstream of the Mountain Stream Zone, there was a significant deterioration in the ecological integrity of all three rivers due to a number of probable causes. Results based on recorded SASS Scores and Average Score per Taxon (ASPT) values, using ‘biological bands’ generated from reference sites in the South Western Cape, were generally similar to and supported by the corresponding multivariate analyses undertaken. From the results of the various analyses undertaken in this investigation and some of the problems encountered in interpreting the data, a number of recommendations are made regarding future bioassessment studies based on the SASS within the national River Health Programme (RHP). To test the IHAS, secondary data were obtained from reference sites in the Mpumalanga and Western Cape Provinces of South Africa. Assuming that SASS Scores at reference sites are the highest scores attainable, one would expect to find a positive relationship between SASS Scores and IHAS scores at reference sites. The assumption in this investigation was that this relationship should be linear. Non-parametric correlation analyses were undertaken between SASS-4/5 Scores and IHAS scores, using Kendall’s Rank-correlation Coefficient (τ), with separate analyses undertaken for different geomorphological zones and biotope groups. Correlations between SASS Scores and IHAS scores were generally weak (τ-values mostly < 0.3) and unsatisfactory, with no significant correlations (p < 0.05) for two-thirds of the data sets analysed and a wide degree of scatter generally observed amongst data points in respective scatter plots. The performance of the IHAS varied between geomorphological zones and biotope groups, with the Foothill: Gravel-bed Zone in Mpumalanga showing the best results, particularly when the stones-in-current biotope group was analysed separately. Further testing of the IHAS is required to confirm its relative performance in different bioregions/ecoregions, geomorphological zones and biotope groups, which should be undertaken as a priority research area within the RHP. Unsuccessful attempts to test the IHAS by means of multiple regression analyses were undertaken, suggesting that such techniques should be avoided in further testing of the IHAS. / AFRIKAANSE OPSOMMING: Die hoofdoel van hierdie studie was om die ekologiese toestand van die Lourens-, Palmiet- en Houtbaairiviere (Suidwes Kaap, Suid Afrika) te bepaal en te vergelyk deur die bestudering van die makroinvertebraatgemeenskapstruktuur by verteenwoordigende monsterpunte langs die riviere, met gebruik van die “South African Scoring System” – Weergawe 5 (SASS-5) snelle biologiese bepalingsmetode. Sekondêre doelwitte het die bepaling van die gevolge van seisoenele veranderlikheid, biotoop beskikbaarheid en ligging-bepaalde omgewingsveranderlikes op die makroinvertebraatgemeenskapstruktuur by monsterpunte ingesluit, asook die inleidende toetsing van die “Integrated Habitat Assessment System” (IHAS) vir watermakroinvertebrate. Volgens die resultate verkry, was die ekologiese toestand van monsterpunte in die Bergstroomsone van die drie riviere konsekwent goed. Die Houtbaairivier in die boonste gedeelte van die Oranjekloofreservaat was veral feitlik onversteurd en hierdie streek is in die studie as ‘n potensiaal biodiversiteit “hot-spot” vir watermakroinvertebrate geidentifiseer. Stroomafwaarts van die Bergstroomsone was daar ‘n beduidende verswakking in die ekologiese toestand van al drie riviere, as gevolg van ‘n aantal moontlike oorsake. Resultate gebaseer op bepaalde “SASS Scores” en ‘Gemiddelde Waarde per Takson’ (“Average Score per Taxon” - ASPT) waardes, met gebruik van ‘biologiese bande’ wat van verwysingsmonsterpunte in die Suidwes Kaap afgelei is, was oor die algemeen soortgelyk aan en gestaaf deur die ooreenstemmende multiveranderlikke (“multivariate”) statistiese analises wat gedoen is. Uit die resultate van die verskeie analises wat in hierdie ondersoek gedoen is en sommige van die probleme wat in die dataverklaring gevind is, is ‘n aantal aanbevelings gemaak met betrekking tot toekomende biologiese bepalingstudies vir die nasionale Riviergesondheidsprogram (“River Health Programme” - RHP) wat op die SASS gebaseer is. Om die IHAS te toets is sekondêre data van verwysingsmonsterpunte in die Mpumalanga en Wes Kaap Provinsies van Suid Afrika verkry. As aangeneem word dat die “SASS Scores” by verwysingsmonsterpunte die hoogste moontlike tellings is wat bereik kan word, sou ‘n positiewe verwantskap tussen “SASS Scores” en IHAS tellings by verwysingsmonsterpunte verwag word. Die veronderstelling in hierdie studie was dat dié verwantskap lineêr moet wees. Nie-parametriese korrelasieanalise tussen “SASS-4/5 Scores” en IHAS tellings is gemaak, deur gebruik van Kendall se Rangkorrelasiekoëffisiënt (τ), met afsonderlike analises vir verskillende geomorfologiese sones en biotoopgroepe verrig. Korrelasies tussen “SASS Scores” en IHAS tellings was algemeen swak (τ-waardes < 0.3) en onbevredigend, met geen beduidende korrelasies (“p” < 0.05) vir twee-derdes van die datastelle wat geanaliseer is nie en ‘n wye verspreiding tussen datapunte in die onderskeie “scatter plots” wat waargeneem is. Die funksionering van die IHAS was verskillend tussen geomorfologiese sones en biotoopgroepe. Die beste resultate is vir die Voorheuwel: Gruisbeddingsone in Mpumalanga verkry, veral indien die klippe-in-stroom biotoopgroep afsonderlik geanaliseer is. Verdere toetsing van die IHAS is nodig om die relatiewe funksionering in verskillende biostreke/”ecoregions”, geomorfologiese sones en biotoopgroepe te bevestig en dit behoort voorangs te geniet binne die RHP. Pogings om die IHAS deur middel van veelvoudige regressie analise te toets het misluk, wat aandui dat sulke tegnieke vermy moet word in verder toetsing van die IHAS.

The high productivity of Eastern Boundary Upwelling Ecosystems (EBUE), some of the most productive ecosystems in the globe, is attributed to the nutrient rich waters brought up through upwelling. Climate change scenarios for coastal upwelling systems, predict an intensification of coastal upwelling winds. Associated with intensification in upwelling are biogeochemical changes such as ocean hypoxia and ocean acidification. In recent years, the California Current System (CCS) has experienced the occurrence of nearshore hypoxia and the novel rise of anoxia. This has been attributed to changes in the intensity of upwelling wind stress. The effects of some of the more severe hypoxia and anoxia events in the CCS have been mass mortality of fish and benthic invertebrates. However, the impacts on zooplankton in this system are not known. Meroplankton, those organisms which have a planktonic stage for only part of their life cycle, are an important component of zooplankton communities. The larval stage of benthic invertebrates forms an important link between benthic adult communities and planktonic communities. Larvae serve to disperse individuals to new locations and to link populations. They are also food for fish and planktonic invertebrates. This important life stage can spend long periods in the plankton (from days to months) where environmental conditions can affect larval health, subsequent settlement and recruitment success, and juvenile health. This research assesses the role of hypoxia and larval survivorship, and the relationship between individual abundance and community structure of larvae to environmental factors in the field. In laboratory experiments (Chapter 2), a suite of 10 rocky intertidal invertebrate species from four phyla were exposed to low oxygen conditions representative of the nearshore environment of the Oregon coast. Results revealed a wide range in tolerances from species with little tolerance (e.g. the shore crab Hemigrapsus oregonensis) to species with high tolerance (e.g. the California mussel Mytilus californianus). The differential responses across larvae to chronic hypoxia and anoxia potentially could affect their recruitment success and consequently, the structure and species composition of intertidal communities. Field studies (Chapter 3 & 4) explore the relationship between environmental variables and larval abundance and community structure. Chapter 3 focuses on broad taxonomic groups, while Chapter 4 focuses on larval decapods in particular. Fine focus was devoted to decapod larvae, due to laboratory findings of heightened sensitivity to hypoxia of decapod crabs. A finding that is also supported in the literature. The goal of field studies was to identify the environmental parameters that structure meroplankton and larval decapod communities and identify which of these parameters play a significant role in influencing larval abundance. A number of environmental variables contributed to meroplankton assemblage structure and larval decapod assemblage structure. These included distance from shore, depth, date, upwelling intensity, dissolved oxygen, and cumulative wind stress. Some of these factors occurred frequently in larval abundance models. In Chapter 3, individual abundance across broad taxonomic groups was most commonly explained by upwelling intensity while in Chapter 4, individual abundance of different decapod species was explained by cumulative wind stress, which is a proxy for upwelling intensity. The prominent role of upwelling related factors in explaining individual abundance is important considering climate change projections of an increased intensification of upwelling winds in EBUE. / Graduation date: 2012 / Access restricted to the OSU Community at author's request from Jan. 6, 2012 - Jan. 6, 2013

Balancing increasing elephant numbers with biodiversity conservation in small reserves has become a concern for many protected area managers. Elephants are considered important agents of disturbance creating heterogeneity and thus contributing to the maintenance of biodiversity. However elephants also damage vegetation through their destructive feeding habits, and this has led to pressure to reduce elephant populations in many reserves. Quantitative data on the impact of elephants on invertebrates, the main component of biodiversity at the species level, are lacking. The aim of this project was to assess the effect that habitat alteration by elephants has on the diversity of selected ground-dwelling invertebrates (ants, centipedes, millipedes, spiders, scorpions and termites) through the provision of logs and dung as a potential refuge niche for these invertebrate communities, and to determine the effect of spatial (vegetation types) and temporal (season and age of dung) variation on the invertebrates using these refugia. Variation in impacts was considered important because savanna is not homogenous and the impact of the refugia is likely to be dynamic in terms of seasonal trends in invertebrate populations, and in terms of changes in the environmental conditions offered by the refugia. Elephant impact on vegetation, quantity of refugia (logs and dung) produced and invertebrate diversity associated with refugia were determined for 115 transects within Madikwe Game Reserve in the North Western Province, South Africa. Invertebrate abundance, species richness and diversity were always higher under refugia than in areas without refugia. Vegetation utilisation, frequency of refugia production and invertebrate diversity showed strong temporal variation (seasonal); elephant impact and production of logs were higher in winter than in summer because elephants are more likely to feed on woody vegetation in winter when grass nutrient levels are low. Invertebrate diversity under the logs was higher in summer than in winter, and this probably reflected the higher abundance and diversity of invertebrates that are usually associated with the warmer, wetter summer months. The effect of adding refugia to three vegetation types on invertebrate diversity was tested experimentally at Makalali Private Game Reserve in the Limpopo Province, South Africa. Logs and elephant dung were set out in five plots each measuring 20m x 20m within Govender - iii mixed bushveld, riverine and mopane woodland. Significant differences were observed in invertebrate abundance, species richness and diversity between the refugia and control plots that lacked refugia and between the three vegetation types sampled. Similarity between invertebrate communities utilising the different refugia types and between the three different vegetation types were tested using the Jaccard similarity coefficient. The three vegetation types shared fewer than 50% of their species, as did the logs, dung and control sites. However the results obtained do illustrate a higher degree of similarity between the refugia substrates (logs and dung) than the control sites and between the more heterogeneous vegetation types (mixed bushveld and riverine) than the mopane veld. This indicated that invertebrate communities associated with refugia were not uniform, but were influenced by vegetation type. An experimental test of temporal changes in invertebrate community composition illustrated the importance of elephant dung as a microhabitat for different invertebrate groups over different ages of dung (three days, two, four, 12 and 32 weeks old). Colonisation of the dung, by dung beetles was immediate but as the microclimate of the dung changed with time, the new conditions were ideal for other invertebrate taxa. Over a period of eight months, the change of invertebrate communities utilising the dung included dung beetles, followed by millipedes and [mally ant and termite communities. The results of this study illustrated the importance of refugia (logs and dung) produced by elephants for ground-dwelling invertebrate species in the savanna environment. The extent of the influence of the refugia varied both spatially and temporally and this should be considered in future monitoring or in measuring impacts. While further research on a broader range of organisms and at larger scales is necessary, elephants do have a positive impact on at least some components of biodiversity, through the process of facilitation of refugia. / Thesis (M.Sc.)-University of KwaZulu-Natal, Pietermaritzburg, 2005.