Supplementary information 追加（2019-09-30) / 京都大学 / 0048 / 新制・課程博士 / 博士(医学) / 甲第21344号 / 医博第4402号 / 新制||医||1031(附属図書館) / 京都大学大学院医学研究科医学専攻 / (主査)教授 柳田 素子, 教授 山下 潤, 教授 江藤 浩之 / 学位規則第4条第1項該当 / Doctor of Medical Science / Kyoto University / DFAM

京都大学 / 0048 / 新制・課程博士 / 博士(医学) / 甲第21688号 / 医博第4494号 / 新制||医||1036(附属図書館) / 京都大学大学院医学研究科医学専攻 / (主査)教授 河本 宏, 教授 生田 宏一, 教授 江藤 浩之 / 学位規則第4条第1項該当 / Doctor of Medical Science / Kyoto University / DFAM

京都大学 / 新制・課程博士 / 博士(医学) / 甲第24129号 / 医博第4869号 / 新制||医||1059(附属図書館) / 京都大学大学院医学研究科医学専攻 / (主査)教授 平井 豊博, 教授 中島 貴子, 教授 森本 尚樹 / 学位規則第4条第1項該当 / Doctor of Medical Science / Kyoto University / DFAM

Granbarkborre är en betydande ekonomisk skadegörare i svensk skog men den är också en art som skapar död ved av försvagade granar. Sommaren 2019 var granbarkborrarna ovanligt många och orsakade stor skada för skogsbruket. I den här etnografiska studien, baserad i Stockholm och närområdet, undersöks hur granbarkborreangreppen sommaren 2019 dels kan förstås som en process av skapande av en sammanhängande värld, dels en möjlighet att i sprickorna hitta öppningar för att driva politik. Med utgångspunkt i att hur problemet med granbarkborre formuleras är viktigt för vilka åtgärder som föreslås undersöks vad vi kan vi lära oss om skogsindustri, natur och biodiversitet.  Att skyddade områden förstås som en orsak till granbarkborreangrepp och att klimatförändringar, älgar och andra varelser är en del av att göra det moderna skogsbruket oförutsägbart framkommer som viktiga resultat. Genom att visa hur den döda vedentillsammans med granbarkborren kontrolleras på vissa platser, visas hur olika kunskapsproduktioner träder fram, något som också underlättas av att mycket runt granbarkborrens livscykel är osäkert. Därför möjliggörs tolkning av naturen istället för konkreta orsakssamband. Uppsatsen är ett bidrag till antropologin om miljöförändringar.

京都大学 / 新制・課程博士 / 博士(医科学) / 甲第24843号 / 医科博第151号 / 新制||医科||10(附属図書館) / 京都大学大学院医学研究科医科学専攻 / (主査)教授 齋藤, 潤, 教授 遊佐, 宏介, 教授 松田, 秀一 / 学位規則第4条第1項該当 / Doctor of Medical Science / Kyoto University / DFAM

No description available.

No description available.

京都大学 / 新制・課程博士 / 博士(医学) / 甲第23752号 / 医博第4798号 / 新制||医||1055(附属図書館) / 京都大学大学院医学研究科医学専攻 / (主査)教授 山下 潤, 教授 木村 剛, 教授 井上 治久 / 学位規則第4条第1項該当 / Doctor of Medical Science / Kyoto University / DFAM

Streptococcus mutans is a facultative member of the oral plaque and is associated with dental caries. It is able to survive long periods of sugar starvation. The purpose of this project was to explore specific avenues that S. mutans may use in order to cope with carbohydrate deprivation. Intracellular polysaccharide (IPS) is accumulated by S. mutans when grown in excess sugar, and can contribute towards the cariogenicity of S. mutans. Inactivation of the glgA gene, encoding a putative glycogen synthase, prevented accumulation of IPS in batch cultures grown with excess glucose or sucrose. Inactivation of the pul gene, encoding a putative pullulanase which is thought to be involved in IPS catabolism, did not prevent IPS accumulation. IPS was found to be important for the persistence of S. mutans grown in batch culture with excess glucose, and then starved of glucose. In these conditions, the IPS was largely used up within one day of starvation, and yet survival of the parental strain was extended by at least 15 days beyond that of the glgA and pul mutants; potentially, some feature of IPS metabolism, distinct from providing nutrients, is important for persistence. IPS was not needed for persistence when sucrose was carbon source or when mucin was present in batch cultures. IPS accumulation was not clearly demonstrated in biofilm conditions. When grown in condition permissive for IPS accumulation, biofilms of the glgA and pul mutants did not show decreased survival, compared to the parental strain. It is plausible that, within a biofilm, S. mutans can use alternative sources of energy (like the extracellular matrix) to compensate for the lack of IPS. To look at specific genes upregulated by sugar starvation, microarrays analysis was performed on S. mutans batch cultures. Some of the genes upregulated by starved, stationary phase bacteria, appeared to be organized in an operon, thought to encode components of the pyruvate dehydrogenase (PDH) complex. Northern Blot analysis showed that pdhD and the downstream genes, pdhA, pdhB and pdhC, form an operon that is transcribed predominantly in stationary phase. Inactivation of pdhD impaired survival of both batch cultures and biofilms. Analysis with fluorescent reporters revealed a distinct expression pattern for the pdh promoter, with less than 1% of stationary phase bacteria displaying pdh expression. When first detected, after one day of sugar starvation, expression was in individual bacteria. At later times, expressing bacteria were often in chains. The lengths of chains increased with time suggesting growth and division. It is likely that the pdh-expressing sub-population is able to persist for extend times in stationary phase. / Microbiology and Immunology

In many natural forests, forest managers fell and debark spruces (Picea abies) colonised by theEuropean spruce bark beetle (Ips typographus) to prevent the beetle from spreading to other tree stands. The aim of this study was to examine how this method affects the biodiversity of other saproxylic beetle species. Eclector traps were installed on debarked and non-debarked dead spruces of four different ages in four nature reserves to compare the richness and composition of saproxylic beetles. The results indicated that a significantly higher number of species and individuals emerged from standing dead trees with bark compared to debarked logs. The highest emergence of species and individuals occurred in one-year-old standing trees with bark. There was a significant interaction between the type and the age of wood, suggesting thatthe richness declined with the aging of wood with bark, while it remained constantly low in debarked logs. The species composition varied greatly between standing trees with bark and debarked logs, as well as between standing trees with bark of different ages. This study demonstrated that debarking spruces as a pest control method reduces the diversity of nontarget saproxylic beetle species. Potential reasons behind that could be the hardening and drying of consumable parts of the wood, rendering it inhabitable for many saproxylic organisms, as well as the presence of the European spruce bark beetle itself, which is associated with many other species.