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31	Einsatz von Mediatoren bei der enzymatischen Aktivierung der fasereigenen Bindekräfte zur Herstellung von enzymgebundenen, bindemittelfreien Holzwerkstoffen / Application of mediators in enzymatical activation of fiberself cohesions for the production of enzyme-bonded, binder-free derived timber products Euring, Markus 20 June 2008 (has links) No description available. 500 Naturwissenschaften Forest Sciences and Forest Ecology Mitteldichte Faserplatten MDF Dämmstoffe Holzwerkstoffe Natürliche Bindemittel bindmittelfrei fasereigene Bindekräfte enzymatische Aktivierung enzymgebunden Enzyme Laccase Mediatoren Laccase-Mediator-System Polymerisation Holzfasern Lignin Weißfäulepilze Medium Density Fiberboards MDF isolating boards derived timber products natural binders binder-free fiberself cohesions enzyme-bonded enzymatical activation enzymes Laccase Mediators Laccase-Mediator-system polymerisation woodfibers lignin white-rot fungi 48 48.46 58 58.29 58.30 58.46 BBF 8 862 862.3
32	An exploration of biochemistry including biotechnology, structural characterization, drug design, and chromatographic analyses Burns, Kristi Lee 28 September 2006 (has links) We now report an in depth analysis of the successful in vitro enzymatic synthesis of PHB utilizing the three-enzyme system from the bacteria Cupriavidus necator. Using HPLC methodology developed in this laboratory, and by adding each enzyme in a step-wise manner, we follow each individual stage in the three-enzyme route for PHB synthesis and delineate all stoichiometric relationships. We report the construction of the first metabolic model developed specifically for analyzing in vitro enzymatic PHB synthesis. We developed a hands-on student laboratory for culturing, producing, isolating, and purifying the bacterial biopolyesters PHB. We now report the first structural characterizations of iso-CoA, acetyl-iso-CoA, acetoacetyl-iso-CoA, and beta-hydroxybutyryl-iso-CoA using MS, MS/MS, and homo- and hetero-nuclear NMR analyses.We describe HPLC methodology to separate the isomers of several iso-CoA-containing compounds and report the first examples of iso-CoA-containing compounds acting as substrates in enzymatic acyl-transfer reactions. We describe a simple regioselective synthesis of iso-CoA from CoA. We also demonstrate a plausible mechanism, which accounts for the existence of iso-CoA isomers in commercial preparations of CoA-containing compounds. Herein we report that phenylaminoethyl selenide compounds protect DNA from peroxynitrite-mediated single-strand breaks. The mechanism of protection against peroxynitrite mediated DNA damage was investigated by HPLC. The chemistry of the reaction between peroxynitrite and HOMePAES was investigated using HPLC and HPLC/MS. The unique chemistry of the reaction between peroxynitrite and HOMePAES was investigated using HPLC and HPLC/MS. We report the development of novel CDB derivatives, which are selective COX-II inhibitors. A series of compounds were assayed with an in vitro colorimetric inhibitor screening and with a whole blood ELISA screening and the results indicate that MST is a selective inhibitor of COX-II. Iso-coa Iso-coenzyme A Poly-(beta)-hydroxybutyric acid PHB Phenylaminoethyl selenide compounds Biopolyesters Acetyl-iso-coa Acetoacetyl-iso-coa Regioselective synthesis Homepaes DNA DNA damage Single-strand breaks Acyl-transfer reactions Culturing Homo-nuclear NMR Purifying Producing Isolating Isomers Selective COX-II inhibitors Whole blood Structural characterizations ELISA In vitro Enzymatic synthesis Beta-hydroxybutyryl-iso-coa MS/MS MS HPLC Metabolic model Hetero-nuclear NMR Peroxynitrite HPLC/MS CDB derivatives Poly-beta-hydroxybutyrate Biosynthesis
33	Planetárium s kavárnou / Planetarium with coffee Vlčková, Sabina January 2016 (has links) Thesis on "Planetarium with coffee" is processed in the form of project documentation for construction. The proposed facility is designed as a two-storeyed without basement with the main ground plan dimensions of 52,8 x 27,47 m and is located near the village Lošov close to Olomouc. The building is operationally divided into a part intended for public entrance and a part for operation and maintenance. Loadbearing structural system is reinforced concrete monolithic skeleton based on monolithic reinforced concrete foundation footings. Ceiling structure consists of reinforced concrete, monolithic, locally supported, cross reinforced floor slab. Vertical external walls are made of ceramic blocks with contact thermal insulation system. Part of the front rounded facade is designed as glass fronted. The building is covered by a warm flat roof. Roof cladding consists of vegetation layers, walkable terrace or non-walkable structure of roof cladding. The planetarium itself is covered by a reinforced concrete dome with a thermal insulation layer of plates of foamed glass. Fillings of doors and windows are made of aluminum profiles with isolating triple glazing. The building meets the requirements for barrier-free use of buildings.

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An exploration of biochemistry including biotechnology, structural characterization, drug design, and chromatographic analyses

Planetárium s kavárnou / Planetarium with coffee