Content of this diploma thesis is analysis of the operation of heat and cold sources and its evaluation based on data from measurement and control systém and design of a new solution for heating and cooling selected part of the object Otevřená zahrada. Building is located on the street Údolní in Brno. It is a three-storey building serving as offices of non-profit organizations and weekend activities of the organization. On the first floor there is a technical background of the building and half of it is used by four offices. On the second floor there is a seminar hall with a capacity of 50 persons. The rest of the floor is used by three meeting rooms and three offices. The third floor is used by four offices. For the heating and cooling of building, there are designed heat pumps ground-water. Air exchange in the building is provided by the air handling unit.

THIS DIPLOMA WORK IS PROJECTING A PROJECT DOCUMENTATION FOR THE CONSTRUCTION OF STUDENT DORMITORY IN BRNO. THE BUILDING IS SITUATED AT THE TECHNOLOGY PARK NEAR TO SEVERAL VUT FACULTY AND STUDENT CAMPUS. THE DESIGN CAME FROM THE LONG-TERM LACK OF ACCOMMODATION CAPACITY ON EXISTING DORMITORY IN BRNO, THE EMPHASIS WAS PUT MAINLY ON THE ACCOMMODATION CAPACITY OF THE BUILDING. THE BUILDING CONSISTS OF TWO FOUR-STOREY BLOCKS WITH A TOTAL CAPACITY OF 244 BEDS, WHICH ARE SITED ON THE 2ND TO 4TH FLOORS. A TYPICAL AND REVERSE ELEMENT IS A LIVING CELL IN WHICH CONTAINS TWO DOUBLE ROOMS - A COMMON SCHEDULE WITH DINING ROOM, A COMMON SOCIAL BACKGROUND WITH A HALL, AND TWO BALCONIES ACCESSIBLE FROM THE KITCHEN. 1. THE FLOOR FLOOR CONNECTS BOTH BLOCKS WITH A LARGE STUDY AND CORRIDOR. THE REMAINING ROOMS IN THE 1ST FLOOR ARE USED AS A TECHNICAL AND ADMINISTRATIVE FACILITY OF THE DUTY.

The subject of my diploma thesis is the elaboration of an architectural study and project documentation for the construction of a detached house building. The new building is located on the outskirts of slavkov, in the district of Opava. It is a three-storey non-cellar building with vertical structures in the Porotherm system, monolithic reinforced concrete ceilings and a single-skin flat roof, vegetation. Ten apartments and four separate rooms are designed in the building and wellness is designed in the first floor.

The dissertation thesis is focused on thermal insulating properties of reflective insulation under boundary conditions typical for low-energy buildings. In the introductory part of this thesis the attention is focused on the heat transfer process. There is described primarily heat transfer by radiation. There is also described the reflective insulation and the methods of determination their thermal performance. The remaining part of the thesis deals with the analysis of the results obtained in experimental measurements. Measurement results are compared with the calculation model. Conclusions for technical practice including possibilities of further research are summarized in the final section.

The diploma thesis examines the influence of insulation of terraced houses on the expenses associated with their operation. Its first part deals with legislation concerning the issue and evaluation of energy performance of buildings. Furthermore, the types and properties of the used thermal insulation, methods of thermal insulation, types of filling of openings and then the used methods of budgeting are described. The second part contains several variants of insulation of terraced houses, which are assessed in terms of energy intensity and finally in terms of economic return on investment in insulation.

In this work the functionalised magnetic particles were tested with streptavidin to selective DNA isolation. The method of selective DNA isolation was tested by using DNA probiotic strain Lactobacillus paracasei subsp. paracasei CCDM 211/06. A test was done on the biotinyl oligonucleotic particles, which was immobilised by containing streptavidin and it was used like a DNA probe for isolation complementary DNA chain by means of DNA/DNA hybridization. The primer R 5´ bio and the biotinyl denatured specific PCR product were tested for species Lb. paracasei as a DNA probe. These following experimental conditions were optimized for selective DNA isolation: temperature and time of hybridization, amount of DNA and the release of DNA from microspheres. Isolation of DNA was verified by PCR with specific generic primers. The specific generic PCR product was amplified in extent 250 bp, which was detected by using electrophoresis in agarose gel. This optimized method was successfully used in selective isolation of DNA Lactobacillus from a complementary sample of supplementary food (BIFI pangamin).

Microbial DNA was isolated from lysed cells of Lactobacillus genus in probiotic products. Reversible adsorption DNA on the surface of carboxyl coated nonporous poly(2-hydroxyethyl methacrylate-co-glycidyl methacrylate) (P(HEMA-co-GMA)) magnetic particles and silicagel coated manganase Perovskite nanoparticles. DNA was adsorbed on the surface of the particles in the presence of 16 % poly(ethylenglycol) (PEG 6000) and 2 M sodium chloride (NaCl) concentrations. The adsorbed DNA was released from particles by low ionic strength TE buffer (pH= 8.0). The quality of isolated DNA was checked by spectrofotometric measurement and PCR amplification. DNA samples isolated using magnetic particles and phenol extraction method (control method) were PCR-ready. The DNA isolated from lysed cells of probiotic products was quantificated in real-time qPCR.

Probiotic lactic acid bacteria of genus Lactobacillus play an important role in the digestive tract of human. They are used in food processing and they are the part of food supplements. Lactic acid bacteria of the genus Lactobacillus can be identificated by polymerase chain reaction (PCR). Bacterial DNA was isolated from cell lysates of 4 synbiotic food suplements by magnetic particles P(HEMA-co-GMA). Isolated DNA was amplified by genus-specific and species-specific primers. Magnetic particles with immobilized antibodies against Lactobacillus bacteria were used in the next part of thesis. These particles were used for isolation target cells from products with their identification by genus specific PCR.

Probiotic lactic acid bacteria (LAB) are very often used in food procesing industry, such as milk products, cheese and fermentsd salami production in nova days. In diploma thesis were tested symbiotic food supplements from different producers. Bacterial DNA was isolated from crude cell lysates of six food suplements by magnetic particles P(HEMA-co-GMA). PCR-ready DNAs were isolated. from all products The detection of Bifidobacterium bacteria identified by PCR was in agreement with those declared by the manufacturers. Magnetic particles with immobilized antibodies against Bifidobacterium were used in the next part of thesis. These particles were used for the isolation of target cells from two products with cell identification by genus specific PCR.

Extraction of nucleic acids is an important step for all molecular biological studies. The process of isolation of plant DNA is complicated due to the presence of polyphenols, polysaccharides and other metabolites. They can be co-isolated with DNA and act as PCR inhibitors. The aim of this study was to compare CTAB extraction procedure, Qiagen DNA easy kit, direct homogenization, carboxyl-functionalised magnetic non-porous HEMA based microspheres and combination of the above mentioned methods for DNA isolation from different plants. The DNA was evaluated regarding concentration, purity and amplification in PCR. All methods provided DNA that could be used in downstream PCR applications. However, there were differences regarding yield, purity, labour intensiveness and cost. Combination of direct homogenization and magnetic microspheres coated by carboxyl groups was isolated DNA from various plants and plant foods in a quality suitable for convectional PCR, real time PCR and restriction analysis. This method is fast, simple and does not require work with harmful substances.