This thesis deals with the production of fruit wines from specified varieties of fruit and selecting complementary suitable grape wines. The thesis also deals with comparison of the wines in terms of their antioxidant content. The first part deals with the literature review, which focuses on the summary of literature sources dealing with fruit wines. It describes the fruits of which were made fruit wines. A further vine wines selected for comparison. Described are substances found in both the fruit and the grape. A separate chapter is devoted antioxidants. It is described technology of wine production. The experimental part is focused on comparing fruit and grape wines in terms of antioxidant content. For the experiment were used made fruit wines (apple, strawberry and elderberry) vine and wine (Müller -Thurgau, Saint Laurent rosé, Blue Portugal). Fundamental analyzes were made of fruit ciders and fruit and grape wines by ALPHA. Method GS / MC were determined by volatile compounds in wines. Spetrofotometricky content was determined the essential antioxidants. Spectrophotometry was determined using two methods Trolox DPPH and DPPH GA, antiradical activity. Part of the evaluation of fruit and grape wines were sensory analysis. Reached the highest levels of antioxidants wines Blue Portugal and elderberry wine. It can say that among the surveyed wines was the difference in the content of antioxidants, which was due to production technology.

Thesis on the topic Antioxidant in herbal teas is divided into two parts. The first part theortical is concerned with the description and division of antioxidants. There are mentioned some synthetic antioxidants, some major intracellular antioxidant and natural antioxidant, which are represented by polyphenolic compounds as the main components of herbal teas. Furthmore, there is described the determination of biologically active compounds by extraction techniques to chromatographic methods. In the theoretical part are also described individual samples of herbs used for analysis. The second part is concerned with the practical determination of polyphenolic acids in selected samples of herbal teas. Two extraction techniques were chosen for separation of polyphenolics acids. The first was Soxhlet extraction accelerated and the second was exctraction shaker. For the determination of polyphenolic acids was used high performance liquid chromatography (HPLC) coupled with a diode array detector (DAD) and with mass detector type triple quadrupole. The measured results show that in most samples of herbs were the most represented chlorogenic acid and caffeic acid.

The aim of this thesis was to find biologically active substances that would be suitable as natural conservatives for herbal sirups, in our case elderflower sirup. Because esential oils are among biologically active substances well known for their antimicrobial aktivity we chose thyme, lemongrass and clove bud essencial oils to be tested on the eldeerflower sirup. Potencial use in a private small scale production was one of the reasons for this particular topic and therefore its technological inovation. The emulgated essential oil was mixed with the sirup and observed for 14 days for the colonies forming units (CFU) which was determined by the drop plate technique. Cultivation was done at a room temperature 22° C as its expected by the consumer that sirups may be stored this way. After the cultivation the numbers of colony forming units were counted and turned into a logaritmic value. Clove essential oil proved a significant difference between control specimen and the essential oil preserved specimen. Statistically remarkable corelation was found also between different concentrations. As well as clove, lemongrass essencial oils proved certain antimicrobiologial activity also with statistical significance. Thyme essential oil proved antimicrobiological activity as well only with no significant difference between concentrations. Essential oils are suitable as natural conservatives for not only their proven antimicrobiological activity but also low price and easy application.

Traditional medicine of indigenous cultures is based on the medicinal effects of local plants. Those people have been taught to use these plants throughout history and they employ them as their primary health care. This is supported by the fact that the natives do not have the financial resources to pay for medical care and at the same time it is also accompanied by poor availability of hospitals in areas where these cultures are located. This thesis covers laboratory verification of antimicrobial and antioxidant activity of Amazonian plants from south west Bolivia. This paper also summarizes the economic and political situation of the country together with the development of traditional medicine in the world and with the potential use of this medicine in pharmacy. Part of the work is devoted to the issue of ever-increasing antimicrobial resistance and the possible effects of free radicals in the human body. The experiment was performed by testing nine samples of imported dried plants in laboratory. To determine the antimicrobial properties a microdilution method was used and the antioxidant activity was verified with the ORAC method which is based on the elimination of free radicals. The microdilution method confirmed 80% of the MIC in the initial concentrations tested (1,024 mg/ml) in Satureja ovata plants against Staphylococcus aureus ATCC 33591 bacteria and Streptococcus pyogenes bacteria and in the plant Baccharis genistelloides against S. pyrogenes bacteria. The other samples have failed to provide the value of the minimum inhibitory concentrations. This can be attributed to several factors which are described in this thesis. In contrast the positive effect free radicals was confirmed in all samples. The highest efficacy showed Satureja ovata (1373,2 ug TE/mg extract). Writing this thesis was accompanied by a lack of information about the tested plants, both in terms of their botanical description and in terms of comparing the laboratory results obtained with the results of specialized studies. Therefore the results of the experiment were studied in comparison to traditional use of the tested plants that have been provided by Bolivian natives or their effects have been analyzed from the literature.

Identification and characterization of plant-based products with antioxidant and anti-proliferative effects has received much interest over the past few years as possible therapeutic mean for treatment of diseases likely to be associated to oxidative stress (such as atherosclerosis, diabetes, and cancer). This study provides characterization of in vitro antioxidant and/or anti-proliferative potential and phytochemical profile of (i) 39 wine samples of underutilized Georgian grapevine cultivars, (ii) extracts of 22 samples of medicinal plants from Ethiopia and (iii) 23 samples of edible and medicinal plants from Peruvian Amazon. For this purpose, 2,2-diphenyl-1-picrylhydrazyl radical assay (DPPH), oxygen radical absorbance capacity (ORAC) assay, total phenolic content (TPC), and cell viability assay based on metabolization of tetrazolium bromide (MTT) to formazan, together with methods based on high performance liquid chromatography (HPLC-ultra violet/visible spectrometry and HPLC-tandem mass spectrometry) were used. Georgian red wines (particularly Saperavi cultivars) exhibited higher antioxidant capacity (average DPPH and ORAC values at 5.1 and = 10.6 g TE/L wine, respectively) in comparison to Central and West European cultivars [Pinot Noir (DPPH = 3.1 and ORAC = 9.4 g TE/L wine), Cabernet Sauvignon (DPPH = 3.0 and ORAC = 7.3 g TE/L wine) and Cabernet Moravia (DPPH = 2.0 and ORAC = 8.5 g TE/L wine)]. Georgian wines contained significantly greater concentrations of quercetin (between 14.44 and 1.07 ug/mL), kaempferol (between 1.68 and 0.03 ug/mL) and syringic acid (between 12.59 and 4.72 ug/mL), whereas possessed lower quantities of resveratrol (between 5.11 and 0.32 ug/mL) in comparison to Central and West European wines. Amongst edible and medicinal plants from Ethiopia and Peruvian Amazon, only Dodonaea angustifolia (IC50 for DPPH = 22.2 ug/mL, ORAC = 767.6 ug TE/mg extract; IC50 for Hep-G2 = 120.0 ug/mL), Rumex nepalensis (IC50 for DPPH = 5.7 ug/mL, ORAC = 1061.4 ug TE/mg extract; IC50 for Hep-G2 = 50.5 ug/mL), Inga edulis (DPPH and ORAC = 337.0 and 795.7 ug TE/mg extract; IC50 for Hep-G2 and HT-29 = 36.3 and 57.9 ug/mL) and Oenocarpus bataua (DPPH and ORAC = 903.8 and 1024.4 ug TE/mg extract; IC50 for Hep-G2 and HT-29 = 102.6 and 38.8 ug/mL) have demonstrated combinatory antioxidant/anti-proliferative efficacy. Selective anti-proliferative activity was observed for Verbascum sinaiticum (IC50 for Hep-G2 = 80.6 ug/mL) and Annona montana (IC50 for Hep-G2 and HT-29 = 2.7 and 9.0 ug/mL, respectively). Above-mentioned plant material showed only weak or non-toxic effects towards normal cell line. Despite the fact that extracts of Jasminum abyssinicum (IC50 for DPPH = 26.3 ug/mL, ORAC = 1023.7 ug TE/mg extract), Rumex nepalensis (IC50 for DPPH = 5.7 ug/mL, ORAC = 1061.4 ug TE/mg extract), Mauritia flexuosa (DPPH and ORAC = 1062.9 and 645.9 ug TE/mg extract), Myrciaria dubia (DPPH and ORAC = 641.9 and 642.6 ug TE/mg extract) and Theobroma grandiflorum (DPPH and ORAC = 714.8 and 821.9 ug TE/mg extract) have exhibited considerable antioxidant effect, these species were found to possess moderate to low anti-proliferative potential or have shown to be toxic to normal cells line. In all cases it was detected that phenolic compounds content correlated strongly with antioxidant activity, however weakly with anti-proliferative effect. Results suggest above-mentioned species as prospective materials for further development of novel plant-based agents effective against oxidative stress related diseases. However, it is necessary to perform further research which would be focused on detailed characterization of their chemical composition, pharmacological effects and toxicological safety, in order to verify their possible practical use.

Hemp is a complex system of chemicals which interacts with one another. The most important chemicals in cannabis are cannabinoids and terpenoids. These compounds evince synergism that makes a number of medicinal effects, e.g. antioxidant, anti-inflammatory, antifungal and analgesic. Volatiles cause aroma of cannabis and have therapeutic effects when it is inhaled. The main of this study was to identify sensory active compounds in the varieties of cannabis by GC-MS/FID and sensory analysis by GC-MS/O. There were 7 samples of varieties to analysis: Cannatonic (CT) Conspiracy Kush (CK) Jillybean (JB) Jacks Cleaner (JC), Jack Skellington (JS) Nordle (N) and Nurse Jackie (NJ). Selection of these varieties was based on its genetic origin - the ratio of morphological types C. sativa and C. indica. Aromatic compounds were extracted by using SPME fiber. Sensory analysis was performed by one trained sniffer. The results show that the most represented volatiles in cannabis varieties (both in C. sativa and C. indica) is myrcene. This terpenoid was in the greatest amount in variety of N (33.4%), NJ (29.8%), CT (29.7%) and JB (29.7%). Then beta-caryophyllene had a large number of presence with high ratio in CK variety (21.1%), JC (20%) and NJ (14%). In the varieties with higher level of morphotype C. indica (CK, N), there were limonene and alpha-humulene represented. Results based on the sensory analysis showed, that sensory activity of limonene, linalool and alpha-humulene is higher in varieties with greater ratio of C. indica. The varieties with a higher ratio of morphotype C. sativa were rich in myrcene and terpinolene.

This thesis deals with the effect of grapevine rootstock on the content of phenolic compounds in-knows them. Phenolic compounds are biologically active substances in the plant, which also, indicate Pohl-free radicals and have strong antioxidant effects. Phenolic compounds are mentioned in connection with beneficial effects on human health. The thesis describes the impact on our most used rootstock for the content of phenolic compounds in wine from white grapes and red varieties Hibernal, Marlen and Cerason. Part of the thesis describes the origin and evolution of phenolic compounds and their effects on plant and positive health effects. The work includes a practical assessment of the impact of various rootstocks content of phenolics, which is undoubtedly the influence proved.

The purpose of the thesis on topic Effect of date of defoliation on aromatic profile ´Sauvignon blanc´ wine was to find out the effect of removing leaves from grapes zone on qualitative and quantitative parameter of grapes of this species. The practical part of the experiment with defoliation in different terms was carried out during 2015 on a vineyard in Svatobořice-Mistřín. Part of the practical experiment was the production of wines from different variants and and evaluated the sensory properties of wine.

The diploma thesis dealt with using oenological tannins in winemaking with research focus being on the Pinot Noir variety. The theoretical part describes the composition of phenolic compounds, classification and origin of tannins which can be found in wine. The practical part researches the use of ten commercially available tannins. The individual samples were evaluated by means of sensory analysis after four months. The assessment included aroma and structure profiles of given wine samples. The next step was the spectrophotometric analysis, which contained and assessed these analyses: total content of phenolic compounds, antiradical activity, total reducing power, total catechin content and total anthocyanin content.

The dissertation concentrated on Chinese cabbage variety comparison - (Brassica pekinensis Ruprecht) and Chinese cabbage (Brassica chinensis L.), that come from Asia, with common varieties grown in the Czech Republic. The main point was to evaluate the nutritive ingredients and morphological and growth parameters. Five varieties were watched according to the individual criteria. These varieties were marked with numbers to be clearer. After successful filling and evaluation of the laboratory results we can say that in the certain chosen parameters the detected values were higher in the varieties that come from Asia. The results concerned C vitamin and other minerals content.