Submitted in fulfilment for the requirements of the Degree in Doctor of Technology: Homoeopathy, Durban University of Technology, 2010. / Homoeopathic provings form the experimental base of clinical homoeopathy. Provings are conducted through the administration of homoeopathically prepared medicine to healthy volunteers in order to elicit disease symptoms. The symptoms are collated to formulate the materia medica of the substance. AIM The aim of this study was to compare the most commonly employed proving methodologies, the C4 trituration, the Sherr and the Dream proving methodology, by application in order to ascertain the validity of the claims made in terms of the efficiency of the method to elicit reproducible symptoms. This study sought to follow the existing methodologies exactly as set out by the original developers with the aim of developing an integrated methodology. The order in which the three groups were assigned followed a logical sequence that ensured that the maximum efficiency would be obtained, and that the blinding process would not be compromised. The claims were investigated based on the hypotheses proving symptoms are reproducible when applying identical proving methodologies in consecutive years, that different methodologies yield different numbers, types and quality symptoms, that differences exist between the symptoms yielded by the placebo and the verum groups within the same methodology, and that an integrated methodology could be developed based on the study of the relative efficiency of the respective methodologies. METHOD During the course of the research, 70 provers were recruited to test the unknown substance through application of the three methodologies Page iii mentioned above. Each of the three groups comprised of 10 verum provers per year, thus totalling 20 verum provers per group. The second group had an additional 10 provers, comprised of five placebo provers for 2008 and 2009 respectively. RESULTS The rubrics produced in each group were statistically analysed. The results reflected a reasonable level of reproducibility, proving the first hypothesis, but highlighted the fact that different provers would result in different symptoms due to their individual susceptibility and sensitivity to the proving substance. This effectively proved the hypothesis that the proving effect was reproducible in consecutive years through the application of the same methodology. The result of the data collection was the formulation of 1 373 rubrics utilised for analysis purposes, resulting in 881 verified rubrics, that comprise the repertory for Protea cynaroides. From the data, it was evident that the C4 trituration and the Sherr proving methodologies yield the most rubrics. Not only do they yield a large number of rubrics, but they also yield a much larger number of rubrics than produced by the placebo portion of the Sherr proving methodology. In the Dream proving methodology group there is much less rubrics present at each rubric level than yielded by the C4 trituration and the Sherr proving methodologies. Strong chapter affinities were observable when applying the C4 and Sherr proving methodologies. The C4 methodology seem to favour the chapters dealing with the senses, evident in the Ear, Eye, Hearing, Mouth, Nose, Skin and Vision chapters where the C4 rubrics were more prevalent than the Sherr rubrics. The Sherr methodology was evident in the remainder of the chapters, indicating the wide applicability of this methodology. This proved the hypothesis that some proving methodologies are more effective than others. Page | iv The hypothesis of difference between the placebo and verum groups within the Sherr proving methodology was proven as it was evident in the number of rubrics produced by each section. The verum portion elicited 63 percent of the total rubrics compared to the placebo portion which only elicited 28 percent. Placebo provers thus elicit fewer symptoms during the proving process than verum provers, demonstrating that homoeopathic drug provings are not a placebo response, but that the administration of the medicine results in the development of clearly observable symptoms in the participants. As originally assumed, the proving did produce clearly observable symptoms in healthy provers. The symptoms gathered through the application of the methodologies were also comprehensive enough to develop a complete materia medica and repertory for Protea cynaroides. CONCLUSION From the data presented in the study, one can thus conclude that in order to elicit symptoms representing all 38 chapters present in the Protea cynaroides proving, the C4 trituration proving and the Sherr proving methodologies would have to be combined. Although Group two is able to elicit the majority of symptoms, it would be even more effective when it is combined with the C4 proving methodology, hence leading to the development of an integrated methodology combining these methods, proving the final hypothesis. The suggested integrated methodology thus comprises of firstly conducting a C4 trituration proving using at least 10 predominantly experienced C4 provers. This proving would serve to highlight the major themes. These themes can then be confirmed through secondly conducting a proving according to the Sherr methodology, in a group comprising of at least 17 provers, including a 10 percent placebo in the group. Repeated oral doses would be administered to the participants in this. At the conclusion of the second Page | v proving stage, all the data would be collated and formatted into a materia medica and repertory. It would, however, be important to prove the integrated methodology‘s usefulness through practical application, leading to the recommendation that the methodology be tested.

Dissertation submitted in partial compliance with the requirements of the Master's Degree in Technology: Homoeopathy, Durban University of Technology, 2010. / The therapeutic effects of homoeopathic Arsenicum album potencies were investigated in-vitro, using human cell cultures which were previously antagonised by arsenic trioxide (As2O3). Primary cell culture (peripheral blood mononuclear cells) and a continuous cell line (MT4) were treated with succussed and unsuccussed homoeopathic potencies, 6CH, 30CH and 200CH. This study aimed to verify the homoeopathic law of similars and to determine whether potencies diluted beyond Avogadro’s constant had physiological effects on cells; whether various potencies would cause different effects as proposed by the Arndt-Schultz law; whether succussed and unsuccussed homoeopathic potencies had different effects on the cells; and to establish whether a biotechnological method could be used to evaluate the above. Initial experiments involved isolation and culturing of the peripheral blood mononuclear cells (PBMCs) and the MT4 cell line. Cell titres were determined using the trypan blue dye exclusion assay. The solubilization method of As2O3 was optimized through various dissolution experiments, so as to attain a homogenous arsenical solution. The MTT assay was used to measure the percentage cytotoxicity and the half maximal inhibitory concentration (IC50) caused by the antagonist As2O3 on the PBMCs and the MT4 cell line. The two cell cultures were compared with regard to their susceptibility to As2O3 and their reliability of response. The homoeopathic potencies of Arsenicum album (6CH, 30CH and 200CH) were prepared by initially triturating the As2O3, and then either hand succussing 10 times (succussed) or allowing to diffuse for 30 s (unsuccussed) in sterile distilled water, with the final potencies made up in cell culture media, RPMI. The MTT assay was used to determine the percentage cell viability when the As2O3-antagonised cells were treated with the Arsenicum album potencies. All assays were performed in triplicate. v The As2O3 was found to fully dissolve when 396 mg of dry As2O3 was added to 100 mL of sterile distilled Milli-Q water, which was left to stand for 10 days at 80°C. The cytotoxicity results showed that the PBMCs were not as reliable as the MT4 cells, which showed significant susceptibility to the As2O3. The IC50 of As2O3 on 1 mL of MT4 cells was found to be 5 μM As2O3 (133 μL) for 48 h. The trypan blue dye exclusion assay demonstrated that the viable MT4 cells decreased in number after exposure to the As2O3, with an increase in number of the non-viable cells. Microscopically, the cells were fewer in number and displayed signs of possible blebbing and cell shrinkage, showing potential cell death due to apoptosis. The cell viability results showed that the Arsenicum album 6CH resulted in the lowest absorbance readings and the Arsenicum album 200CH gave the highest readings; this verified the therapeutic effects of homoeopathic remedies when given according to the law of similars; that potencies diluted beyond Avogadro’s constant had stimulating effects; and that the more dilute potencies stimulated recovery in the cells more than the lower potencies, verifying the Arndt-Schultz law. The treatments and the times of exposure were found to be statistically significant determinants of cell viability, whereas succussion did not cause any significant variation in the results. The study thereby provided evidence that a biotechnological method could be used to scientifically evaluate the physiological effects of homoeopathic potencies on human cells; that the homoeopathic potencies did have therapeutic effects; and that succussion was not required in the potentization method in order to produce a curative remedy.

Mini-dissertation submitted in partial compliance with the requirements for the Master’s Degree in Technology: Homoeopathy, Durban University of Technology, 2010. / The purpose of this study was to investigate the homoeopathic indications of the bark of the Strychnos henningsii tree, through a homoeopathic proving, and to subsequently compare these results to the documented traditional use of the crude substance. Methodology The study design took the form of a triple-blind placebo-controlled trial consisting of 32 participants who met defined inclusion criterion. These participants were randomly divided into four groups, each consisting of eight provers and supervised by one of four M. Tech Homoeopathy students. Fifty percent received placebo and fifty percent received verum. Neither the researcher nor the provers were aware of the nature of the proving substance as well as who received the placebo and who received the verum until the study was completed. The proving substance was administered in the 30CH potency, according to an ECH (European Committee of Homoeopathy) methodology. All provers were subjected to pre – and post -proving consultations in which case histories and physical examinations were performed. A pre-proving workshop was held for all provers and supervisors. Symptom collection was done by using information obtained from prover journals which was then collated, edited and translated into repertory language. By identifying broad areas of traditional use of Strychnos henningsii and relating these to rubrics derived from the proving of Strychnos henningsii 30 CH, a comparison was performed in order to evaluate the overlap between the homoeopathic indications and traditional use of the proving substance. Results After critical evaluation, it was found that whilst overlaps exist in the treatment of liver disease, nausea, dysmenorrhea, rheumatism, chest pain, malaria and dizziness, no overlap was found in the use of the homoeopathic remedy in snakebites. In addition the proving revealed symptoms that were not indicated in traditional use such as headaches, asthma, influenza, sinusitis and hayfever, as well as infections of the eye, ear, nose and throat. Conclusion Thus, upon comparison of the traditional uses of Strychnos henningsii to the symptoms produced in the proving of Strychnos henningsii 30CH, revealed that similarities pertaining mainly to the gastro-intestinal system, female gynaecological tract and rheumatic complaints exist. However, the proving elicited many new symptoms that are purely homoeopathic, these included head, mind, sleep, dream, nose, ear, chest and respiratory symptoms. Furthermore, it is hoped that further provings of this substance at different potencies would contribute further to the materia medica of the substance.

Dissertation submitted in partial compliance with the requirements of the Master’s Degree in Technology: Homoeopathy, Durban University of Technology, 2010. / The homoeopathic drug proving of Strychnos henningsii 30CH took the form of a mixed-method triple-blind, placebo-controlled study. The purpose of the study was to compare the results of the triple-blind homoeopathic drug proving with the toxicology of the crude substance. Methodology Thirty-two provers were selected and randomly divided into four equal groups of 8 provers, with each group supervised by one of four M.Tech.Hom student researchers. Fifty percent of the subjects (16 or the 32) received placebo in a random manner. Provers recorded their state in journals for one week prior to commencing the verum/ placebo powders. Data was extracted from journals and combined with case histories, physical examinations to compile the proving profile. The data derived from journals and case histories were edited and collated and thereafter reformatted and classified according to standard materia medica and repertory conventions. This constituted the homoeopathic remedy picture which was then compared to the toxicity of the major chemical constituents of Strychnos henningsii by qualitative methods. Results Provers produced symptoms on the mental, physical and emotional levels. There was a distinct similarity between the toxicology of Strychnos henningsii in its iii crude form, and a majority of the symptomatology obtained during the proving. The comparison of Strychnos henningsii proving symptoms and that of the crude substance was demonstrated by the proving symptoms showing similarity with the chemical constituents of the bark. The bark contained alkaloids which demonstrated similar symptoms in poisonings as described by the provers. The alkaloids found in the Strychnos henningsii bark were strychnine, diaboline, holstiine, strychnochromine and guianensine. Conclusions This homeopathic proving of Strychnos henningsii allowed for successful comparison between the alkaloids contained in the bark and the proving symptomatology. Notwithstanding the successful comparison and noted levels of overlap, it was observed that prior knowledge of homeopathy was beneficial to the provers. Furthermore, the researcher argues that the triple blind method employed is effective, but may hinder the researcher during analysis and that it is important that toxicological information about any substance should be obtained before a proving commences.

Dissertation submitted in partial compliance with the requirements for the Master‟s Degree in Technology: Homoeopathy, Durban University of Technology, 2011. / Until recently, various attempts have been made to simplify the prescription process in homoeopathic prescribing. The doctrine of signatures, miasmatic theory, the homoeopathic repertory and more recently, kingdom analysis by authors such as Sankaran (1994) and Scholten (1993) are some of the attempts that have been made to understand the materia medica. With the materia medica constantly expanding and considering that plants make up a significant percentage of the materia medica (Kayne, 2006), it is evident that new systems of homoeopathic prescribing are continually needed to help practitioners both study the remedies and prescribe more accurately. Aim The Solanaceae plant family are an important and well utilised plant family in homoeopathy (Vermeulen, 2004). Considering this, it was felt that a study investigating the relationship of the natural history of the family to its general and mental symptoms be conducted in order to apply a previously unexplored research paradigm in order to create a greater understanding of Solanaceae remedies utilised in homoeopathy. The study conducted was a non empirical correlation study of the Solanaceae plant family‟s natural history and general and mental symptoms manifested in Solanaceae remedies utilised in homoeopathy. The aims of the study were to establish if commonalties existed between general and mental symptoms of individual remedies belonging the Solanaceae family and their natural histories, as well as to establish if collective commonalities and correlations existed between the general and mental symptoms and the natural history of the Solanaceae family as a whole. Methodology The homoeopathic remedies obtained from the Solanaceae family of plants for the study were analysed in terms of rubric representation (size) using homoeopathic software packages, Radar 10.4 (Archibel, 2009b) and v Encyclopedia Homoeopathica (Archibel, 2009a) a sample selection was chosen. This selection was analysed in terms of general and mental rubrics. Qualitative thematic analysis was used to establish commonalities in keyword concepts between the respective natural histories of the studied family and their respective general and mental symptoms. Keywords obtained from data tables which included criteria such as habitat and distribution, plant description, active principles (primary alkaloids), uses, physiological action if ingested, historical significance, mythology and toxicology were subjected to thesaurus consultation and tabulated in an attempt to identify synonyms relating to the general and mental symptoms of individual remedies of the sample group in the study. This facilitated in the grouping of similar themes. Once commonalities pertaining to each individual species and remedy was further tabulated and discussed in terms of keywords relating to their natural histories, a collective analysis of common correlations between the plant family as a whole was performed. Results Common themes related to general and mental symptoms and to the natural histories of species in the study included “aggression” found in Atropa belladonna, “depression” found in Solanum dulcamara, “anxiety” found in Datura stramonium, “confusion” found in Hyoscyamus niger, “burns” found in Capsicum annuum, “ convulsions” found in Nicotiana tabacum , “ hallucinations” found in Mandragora officinarum and “delirium” found in Solanum nigrum. Common themes relating to general and mental symptoms and the natural history of the Solanaceae plant family as a whole included convulsions, hallucinations, confusion and anxiety. These themes were further compared to themes exhibited in Solanaceae studies conducted by Mangialavori (2007) and Sankaran (2002).

Dissertation submitted in partial compliance with the requirements for the Master’s Degree in Technology: Homoeopathy , Durban University of Technology, 2011. / The treatment with homoeopathic remedies, namely Phosphoricum acidum 200CH has proved to be effective in many clinical situations; however confirmation of its use within the sporting industry is limited. This study aimed to test Phosphoricum acidum 200CH efficacy in treating highly trained cyclists. This study was a superiority trial, in which the homoeopathic intervention was hypothesized to be superior to the placebo control group, in a statistically significant way (p < 0.05). Aim This study aimed to test the use of Phosphoricum acidum 200CH in treating the adverse physiological and psychological symptoms induced by exercise on cyclists. The aim for treating these adverse symptoms, induced by exercise, is to enhance performance and recovery of cyclists. Performance and recovery were tracked by assessing blood lactate concentration, oxygen consumption rate, heart rate, peak power output and emotional status. Methodology The study was a randomized controlled clinical trial, parallel group design. Participants were selected using convenience sampling of male road and mountain bike cyclists in the Western Cape. This study followed an explanatory Randomized Controlled Trial test, where the efficacy of the homoeopathic remedy (Phosphoricum acidum 200CH) was under investigation. The participants were selected with great care and testing was completed under highly controlled conditions. Thirty competitive male cyclists volunteered for this study. All participants were required to complete two cycling power to exhaustion interval tests, consisting of a ten minute warm-up at 100 Watts, followed by a five minute constant load at 150 Watts. The purpose of the constant load was to test cycling economy (CE). Thereafter the workload was increased to 200 W for 30 seconds and then the workload was increased by 20 Watts every 30 seconds. The test continued until the participant could no longer maintain the set repetitions per minute for that workload. The peak power output attained iv was recorded. The first test served as a baseline, after which a single dose of Phosphoricum acidum 200CH or identical placebo was administered, a 30 minute recovery period allowed for the remedy to take effect. The test interval was then repeated, the second test results were then compared to the first baseline test to determine the effect of treatment. The study took many different aspects of the remedies action on the cyclists into account, including mental and physiological effects. Breath-by-breath gases were continuously recorded. Expired gases, volumes and air flow were sampled through a flow meter and gas sampling line and heart rate was measured through telemetry (Polar®, Polar Electro, Oy, Finland) and analyzed by a cardio-pulmonary metabolic system (Quark CPET ® Cosmed, Rome, Italy, 2009). Data recorded was filtered for values outside the normal ranges and averaged for every five seconds. Oxygen consumption (vO2 mL.min-1) and heart rate (bpm) at different stages of each test interval were recorded. More specifically oxygen consumption and heart rates were averaged over the 5 minute section following the warm-up to determine cycling economy, directly after exhaustion for one minute and two minutes following exhaustion for another one minute period. Maximum attained heart rate was recorded for each interval test. Maximum oxygen consumption (vO2Max Absolute) was calculated as the mean of the highest three values attained, this mean was then divided by the participants body mass to determine maximum aerobic capacity (vO2Max Relative). Blood lactate levels were tested before, 15 minutes into and directly after each interval, to assess resting lactate status, cycling economy and to determine maximum lactate accumulation. Psychological testing included mood analysis, using a Stellenbosch mood scale (STEMS) questionnaire and further symptoms were analyzed using a Numerical rating scale (NRS) with symptomatic questions. Results From the results, it was clearly apparent that the control group proved to be of a higher calibre when comparing performance variables of the two groups namely:  Higher peak power output  Higher vO2Max  Lower economy lactate v  Lower resting lactate Despite the treatment group being the weaker of the two groups, they showed improvement in performance after administration of the remedy. This improvement was manifest through physiological alteration in the second test. More exclusively is the acceptance of the hypothesis concerning heart rate and oxygen consumption, whereby results showed that the administration of Phosphoricum acidum 200CH decreased heart rate and submaximal oxygen consumption rates during performance and recovery. There was no observable psychological effect during this study. The results suggest that Phosphoricum acidum 200CH primary demonstrated physiological effects on the cyclists. The researcher believes that this is due to insufficient time given for psychological alterations. Conclusion The Phosphoricum acidum 200CH has proven to be effective in enhancing cycling economy, reducing maximum heart rate and enhancing recovery to a large degree for the first minute following exhaustion. These positive effects are of great importance as the treatment group was the weaker of the two groups. Resulting in the possibility of even larger results being observable in repeated studies where both groups have similar performance abilities.

Mini-dissertation submitted in partial compliance with the requirements for the Master's Degree in Technology: Homoeopathy, Durban University of Technology, 2010. / Introduction The aim of the study was to determine the effect of Acridotheres tristis 30CH on healthy volunteers (provers) and to record the clearly observable signs and symptoms produced, so that Acridotheres tristis 30CH may be prescribed according to the Law of Similars, as required by homoeopathy. Methodology The proving of Acridotheres tristis took the form of a randomised, placebocontrolled trial on 30 healthy volunteers who met inclusion criteria. The 30th centesimal potency of the tail feather was administered as lactose powders and fifty percent of provers were randomly administered an identical placebo-control substance. The collection of the data from the provers took the form of a journal in which each prover‟s symptoms were recorded for six weeks, including a one-week observation period prior to taking the powders, and a proving period of five weeks after administration of powders. On completion of the proving, each journal was assessed by the researcher to determine the suitability of the recorded symptoms for inclusion in the materia medica of Acridotheres tristis. These symptoms were then translated into the language of the materia medica and repertory and the remedy picture then formulated. Data from case histories, physical examinations and group discussions were also taken into account during the analysis of the proving symptoms. Results The homoeopathic drug proving of Acridotheres tristis, conducted as a doubleiii blind, randomised and placebo-controlled study produced a wide range of symptoms. In the collated edited data arising from the proving 396 journal entries were extracted and 595 rubrics in total were formulated, of which 56 rubrics were new. The main symptoms belonging to the mental and emotional spheres of this remedy include depression, anxiety, memory weakness and isolation accompanied by a need for solitude. The characteristic physical symptoms include headaches, dizziness, extreme fatigue, skin eruptions especially pimples and rashes, haemorrhoids, numbness and severe dysmenorrhoea. Other symptoms indicate a possible use in the treatment of gastro-intestinal complaints which include nausea, abdominal cramps, abdominal distention and heartburn. Conclusion The investigation supported the hypothesis that Acridotheres tristis would produce clearly observable signs and symptoms in healthy volunteers. It is essential that the proving symptoms be verified and expanded through clinical trials and further provings of Acridotheres tristis in various potencies, so that it becomes well-utilized remedy in the future.

Submitted in partial compliance with the requirements of the Master’s Degree in Technology: Homoeopathy, Department of Homepathy, Durban University of Technology, Durban, South Africa, 2015. / Introduction This study was a double-blind, placebo controlled proving of Panthera leo 30CH (claw of a African lioness); the aim being to determine the effect of this substance induces on healthy persons and in doing do explore the homoeopathic therapeutic potential thereof, in addition a subsequent comparison with the provings of Lac leoninum (milk of a lioness) (Sankaran,1998) (Herrick,1998) was performed. Objectives The primary objective of this proving was to determine the effect that Panthera leo 30 CH on healthy provers so that the therapeutic potential of the substance could be determined. The secondary objective was to compare the Materia medica of Panthera leo with that of Lac leoninum (Herrick, 1998) and Sankaran (1998). Lastly, the third objective was to further develop the homoeopathic Materia medica by adding the remedy picture of Panthera leo. Methodology The drug proving of Panthera leo was conducted as a randomized, exploratory, double blind placebo controlled study. Thirty participants (healthy volunteers) who met the inclusion criteria (Appendix B) participated in the proving, twenty – four were given the verum and the remaining six placebo. Upon obtaining written informed consent the researcher performed a full case history and comprehensive physical examination on each prover before commencement of the proving. A subsequent follow up case history and physical examination at the end of the proving was also performed to establish whether provers had returned to their respective healthy states. The provers took their first dose of the proving remedy at a dosage of 1 dose three times daily for 2 days and recorded all symptoms they experienced in their respective journals according to the guidelines in Appendix C. On completion of the proving, all provers handed in their journals which were then transcribed, assessed and analyzed. An extensive comparison between the proving of Panthera leo and the two provings of Lac leoninum (Herrick, 1998) (Sankaran, 1998) followed. The respective remedies were compared and contrasted with respect to Materia medica and repertory and similarities and differences highlighted. Results A total of 1255 rubrics were obtained of which the mind section of the Materia medica weighed heavily comprising 35 % (435 rubrics) of the proving symptoms, the generals section (11% = 135 rubrics) and head section (9% = 109 rubrics) respectively. Therefore it can be suggested that this remedy’s sphere of action is limited largely to the mind (emotional state) however unique physical indications specifically regarding headaches are also within its sphere of action. Of the total, 1255 rubrics that were obtained only 11% (138 rubrics) where shared with the two other provings of the African lion (Herrick 1998 & Sankaran 1998); as suggested by Naude (2011) one can speculate that these shared symptoms may represent the unique mammalian component of the animal the differences however considerably outweigh the similarities between these provings and for this reason one can conclude that factors in addition to the species of animal from which the remedy is sourced further influence the resultant proving symptoms. Conclusion It can be concluded that Panthera leo 30CH produced clearly observable symptoms in healthy provers who participated in this proving, furthermore there was clear congruency between certain aspects of this proving and that of Herrick and Sankaran however such similarities were limited.

Submitted in partial compliance with the requirements of the Master’s Degree in Technology: Homoeopathy, Department of Homeopathy, Durban University of Technology, Durban, South Africa, 2015. / INTRODUCTION A homoeopathic drug proving was conducted by Master’s students at the Durban University of Technology (DUT). The proving substance was nail shavings obtained from an African lioness (Panthera leo). The primary objective of this study was to identify the symptoms produced by healthy individuals in response to Panthera leo 30CH; this was done by administering the proving remedy (Panthera leo 30CH) to healthy individuals, who recorded the symptoms they experienced after taking the remedy. The secondary objective was to compare the symptoms produced to the ethology of the African lion. The final objective was to further develop the materia medica by adding the remedy picture of Panthera leo. METHODOLOGY This study was qualitative and exploratory in nature. The study took the form of a randomised, double-blind and placebo controlled trial. A total of 30 provers, who met the inclusion criteria (Appendix C), participated in this study. The provers were randomly assigned to either a verum or placebo group, such that 24 received verum (Panthera leo) and 6 received placebo. Neither provers nor researchers had knowledge of which provers were in each group until the study was complete, and provers did not have any knowledge regarding the nature of the proving substance. Before commencing the study all provers signed relevant information and consent documents. The researcher then took a full case history and performed a physical examination. Provers recorded their baseline state for 7 days in a journal, prior to taking the verum or placebo, as administered to them. After recording their baseline state for 7 days, they took their remedies and continued to record their symptoms for a minimum of 2 weeks, or until the proving symptoms ceased. Once they had completed recording in their journals, a post-proving case history was taken and a physical examination was performed. All journals were collected and analysed by the researcher. The remedy was manufactured by triturating the crude substance up to the 3rd centesimal potency. This was then converted to a liquid potency and further diluted up to the 30th centesimal potency. Neutral granules were impregnated with Panthera leo 30CH, and dispensed as 10 granules in 500mg of lactose powder. The placebo was dispensed as 10 granules, which were impregnated with 96% ethanol, in 500mg lactose powder. Each prover received 6 lactose powders, containing either verum or placebo. The manufacture of Panthera leo was done according to the methods and guidelines stipulated in the German Homoeopathic Pharmacopoeia (Benyunes, 2005) RESULTS The proving of Panthera leo 30CH produced a total of 1234 rubrics, which represent a wide variety of mental, emotional and physical symptoms. The main themes that emerged from the mental and emotional symptoms included anger, aggression, irritability, poor concentration and fear. Physical symptoms included catarrh, constipation, diarrhoea, diminished appetite, fatigue, insomnia, thirstiness and headaches. A comparison was made between the symptomatology of Panthera leo and the ethology of the lion. Some of the themes of symptoms which corresponded with the ethology of the lion included ‘anger’, ‘audacity’, ‘ennui’, ‘increased appetite and thirst’, ‘responsibilities’ and ‘children’. CONCLUSION The objectives of this study were fulfilled, as the proving of Panthera leo produced a wide variety of clearly identifiable and observable symptoms amongst the healthy individuals recruited. There was a remarkable correlation of a portion of the materia medica with the ethological features of the African lion. As a result of this study, the materia medica of Panthera leo was well outlined and will be added to the existing body of homoeopathic materia medica for application and utilization in clinical practice

Submitted in partial compliance with the requirements for the Master’s Degree in Technology, Department of Homoeopathy, Durban University of Technology, Durban, South Africa, 2015. / INTRODUCTION Homoeopathy is based on the law of similars meaning the medicine that produces symptoms in a healthy individual will cure the same symptoms in a sick individual (Sankaran, 1991:5). AIM Conducting a proving on Ubiquinone 30CH will lead to an establishment of its therapeutic potential through the application of the law of similars thus adding to the Materia Medica and advancing Homoeopathy (Vithoulkas, 2002). It was hypothesised that the 30CH potency of Ubiquinone would clearly produce observable signs and symptoms in healthy prover’s. It was further hypothesised that a comparison of Ubiquinone to those remedies yielding the highest numerical value and total number of rubrics on repertorisation of the proving symptoms would elucidate differences and similarities between Ubiquinone and other Homoeopathic remedies to clarify its therapeutic indications. It was hypothesised that in this manner a better understanding of Ubiquinone and its relationship to other Homoeopathic remedies would be gained. Methodology The proving of Ubiquinone 30CH was a randomised, double blind placebo controlled study, using the 30th centesimal potency and a total of 26 participants who met the inclusion criteria. Each prover was provided with a journal to record their symptoms daily. The data extracted from the journals were added to the case histories and physical examinations to compile a proving profile. The identity of the substance was revealed and the information was correlated after completion of the proving. The symptoms found were translated into Materia Medica and repertory language. Once the proving was concluded, a comparison to the remedies yielding the highest numerical value and total number of rubrics on repertorisation - which is the technique of using a repertory to identify the Homoeopathic medicines whose Materia Medica corresponds most closely to the clinical picture of the patient and from amongst which a simillimum may be chosen (Swayne, 2000:183) - was compared to the proving symptoms. Results The remedy’s main influence was on the mental and physical state. The most prominent symptoms seen in the mental sphere were extreme irritability and exhaustion. There was a sense of emotional fragility with a desire to be alone. On the physical side, headaches were common and weakening pains of the extremities were experienced. It can be concluded that the 30CH potency of Ubiquinone, if used precisely according to Homoeopathic principles, can be applied to a clinical setting, as the extensive range of symptoms produced during the proving suggests an equally wide array of application of the remedy Ubiquinone. Conclusion One of the downfalls of Homoeopathy is the limited number of provings being done, (Vithoulkas, 2002). Vithoulkas (2002:143) maintains that in order for Homoeopathy to advance, it is necessary to perform provings on new substances to expand the Homoeopathic armamentarium. Increasing the number of remedies in the Materia Medica facilitates greater accuracy and individualisation when treating patients (Wright, 1999). According to Herrick (1998) numerous cases cannot be solved because many of the most important remedies have not yet been developed. The purpose of this study was to increase the knowledge of drug substances due to the limited amount of information in our current Materia Medicas, by investigating the therapeutic potential of Ubiquinone 30CH. The investigation supported the hypothesis that Ubiquinone would produce clearly observable signs and symptoms in healthy volunteers. It is essential that the proving symptoms be verified and expanded through clinical use and with further proving of Ubiquinone in various potencies so that it becomes a well utilised remedy in the future.