The described experimental model of clonal tumors induced through the insertional mutagenesis with MAV-2 proved to be a valid and rich source of information describing the process of transformation of normal into tumor cell. We have mapped more than 2000 individual clonal VISs from several hundreds of tumor tissue samples. We have analyzed five tumor types of different histology and tissue of origin along with their derivative tissue cultures. Furthermore, we have discovered the industasis phenomenon and described it during the course of the study. The goal of my study was to uncover common reasons for neoplastic transformation of the cell. The results of my study led me to the paradoxical conclusion that the significance of genetic changes as the primary cause of induction of neoplastic transformation is being overestimated. Although studying the functions of individual genes and search for new tumor markers and therapeutical targets are still beneficial, I believe that the traditional perception of tumor formation as a function/result of mutation accumulation and selection is becoming a serious drawback in further investigations. These conclusions are further discussed in the last section of the presented Ph.D. thesis.

Small Unmanned Aerial Vehicles (SUAVs) are an attractive choice for many surveillance tasks. However, video from an SUAV can be difficult to use in its raw form. In addition, the limitations inherent in the SUAV platform inhibit the distribution of video to remote users. To solve the problems with using SUAV video, we propose a system to automatically create geo-referenced mosiacs of video frames. We also present three novel techniques we have developed to improve ortho-rectification and geo-location accuracy of the mosaics. The most successful of these techniques is able to reduce geo-location error by a factor of 15 with minimal computational overhead. The proposed system overcomes communications limitations by transmitting the mosaics to a central server where there they can easily be accessed by remote users via the Internet. Using flight test results, we show that the proposed mosaicking system achieves real-time performance and produces high-quality and accurately geo-referenced imagery.

Miniature Aerial Vehicles (MAVs) are quickly gaining acceptance as a platform for performing remote sensing or surveillance of remote areas. However, because MAVs are typically flown close to the ground (1000 feet or less in altitude), their field of view for any one image is relatively small. In addition, the context of the video (where and at what orientation are the objects being observed, the relationship between images) is unclear from any one image. To overcome these problems, we propose a geo-referenced mosaicing method that creates a mosaic from the captured images and geo-references the mosaic using information from the MAV IMU/GPS unit. Our method utilizes bundle adjustment within a constrained optimization framework and topology refinement. Using real MAV video, we have demonstrated our mosaic creation process on over 900 frames. Our method has been shown to produce the high quality mosaics to within 7m using tightly synchronized MAV telemetry data and to within 30m using only GPS information (i.e. no roll and pitch information).

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This dissertation presents shape and structural optimization studies on flapping wings for micro air vehicles. The design space of the optimization includes the wing planform and the structural properties that are relevant to the wing model being analyzed. The planform design is parameterized using a novel technique called modified Zimmerman, which extends the concept of Zimmerman planforms to include four ellipses rather than two. Three wing types are considered: rigid, plate-like deformable, and membrane. The rigid wing requires no structural design variables. The structural design variables for the plate-like wing are the thickness distribution polynomial coefficients. The structural variables for the membrane wing control the in-plane distributed forces which modulate the structural deformation of the wing. The rigid wing optimization is performed using the modified Zimmerman method to describe the wing. A quasi-steady aerodynamics model is used to calculate the thrust and input power required during the flapping cycle. An assumed inflow model is derived based on lifting-line theory and is used to better approximate the effects of the induced drag on the wing. A multi-objective optimization approach is used since more than one aspect is considered in flapping wing design. The the epsilon-constraint approach is used to calculate the Pareto optimal solutions that maximize the cycle-average thrust while minimizing the peak input power and the wing mass. An aeroelastic model is derived to calculate the aerodynamic performance and the structural response of the deformable wings. A linearized unsteady vortex lattice method is tightly coupled to a linear finite element model. The model is cost effective and the steady-state solution is solved by inverting a matrix. The aeroelastic model is used to maximize the thrust produced over one flapping cycle while minimizing the input power. / Ph. D.

De nos jours, l'utilisation des drones miniatures à voilure tournante pour des missions d'observation dans des environnements hostiles est en pleine expansion. Ces appareils, grâce à leurs capacités à combiner le vol de translation avec le vol stationnaire, sont en effet bien adaptés aux besoins de ces missions. L'étude présentée dans cette thèse concerne un nouveau concept de drone appelé GLMAV (pour Gun Launched Micro Aerial Vehicle), qui consiste à rendre très rapidement opérationnel un véhicule hybride projectile - drone. La difficulté dans le pilotage de ce type de véhicules est d'assurer de bonnes performances de suivi de trajectoires tout en garantissant une résistance aux perturbations aérodynamiques. Après une étape de modélisation, le coeur de la thèse présente plusieurs stratégies de commande, aussi bien linéaires que non linéaires, permettant la navigation autonome du drone. Plusieurs approches permettant l'estimation et la prise en compte dans la commande des efforts parasites liés aux phénomènes aérodynamiques sont également détaillées. L'efficacité de tous les algorithmes de commande est ensuite illustrée par de nombreuses simulations numériques. Du point de vue pratique, une simple loi de commande ne suffit pas. En effet, des techniques de filtrage particulières ou des aménagements spécifiques doivent être utilisés pour reconstruire l'état du drone. Les performances de l'ensemble de la boucle de commande sont d'abord testées en simulation avant l'implantation sur le prototype du GLMAV développé par l'Institut franco-allemand de recherches de Saint-Louis / Nowadays, the use of rotary-wing MAV for observation missions in hostile environments is constantly growing. These aircrafts, through their ability to perform both translation flights and hover, are indeed well appropriate for these missions. The study presented in this thesis deals with a new MAV concept called GLMAV (for Gun Launched Micro Aerial Vehicle), which consists in getting very quickly up and running a projectile - MAV hybrid vehicle. The difficulty in controlling such vehicles is to ensure good trajectory tracking performances while guaranteeing robustness towards aerodynamic disturbances. After a modelling stage, the heart of the thesis introduces various control strategies, both linear and nonlinear, for the autonomous navigation of the MAV. Several approaches allowing the estimation and the consideration into the control of the parasitic efforts caused by aerodynamic phenomena are also detailed. The effectiveness of the control algorithms is then shown through many numerical simulations. From a practical point of view, having a control law is not enough. Indeed, special filtering techniques or specific equipments have to be used to reconstruct the system state. The performances of the overall control loop are firstly tested in simulation before its implementation on the GLMAV prototype developed by the French-German research Institute of Saint-Louis

Two micro air vehicles, namely Sapthami and Sapthami-flyer, are developed in this thesis. Their total weight is less than 200grams each. They fit inside a 30cm and 32cm sphere respectively and carry the commercially available Kestrel autopilot hardware. The vehicles have an endurance of around 20-30 minutes. The stall speed of Sapthami is around 7m/s and that of Sapthami-flyer is around 5m/s as found by nonlinear modeling. The low stall speed makes it possible for them to be launched by hand. This enhances their portability as they do not require any launching equipment. The vehicle installed with Kestrel autopilot system is capable of many modes of operations. It is capable of fully autonomous flight with the aid of a variety of sensors like the GPS unit, heading sensor, 2-axis magnetometer, 3-axis accelerometer and 3-axis gyros. The vehicle carrying the Kestrel autopilot hardware is capable of autonomous and semi-autonomous flights after installation and tuning of feedback loops. Sapthami, is a tailless flying wing with an inverse zimmermann profile. A flying wing is a preferred configuration for the MAV as it maximizes the lifting area for a given size constraint. For a maximum size constraint of 30cm and aspect ratio around 1, the vehicle operates at Reynolds number between 100,000 to 250,000, at flight velocity 7 m/s to 15 m/s. The Inverse Zimmerman profile has a higher lift coefficient, CL, in comparison to the other planforms such as rectangular, elliptical and Zimmermann, for aspect ratio 1 to 1.25 and tested at Reynolds number of 100,000. The configuration of Sapthami is clean as there is no fuselage and all the components like autopilot hardware and battery are housed inside the wing. A thick reflex Martin Hepperle (MH) airfoil MH18 is chosen which gives sufficient space to place the components. This airfoil is specially used for tailless configurations due to its negative camber at the trailing edge. This negative camber helps in reducing the negative pitching moment of the wing, since no separate horizontal tail is available on a tailless aircraft to compensate for it. The vehicle is fabricated using the blue foam, having a density of 30kg/m3 . The wing is fabricated by CNC machining after which slots are cut manually to embed the electronics. The vehicle is found to have stable flying characteristics. Limited flight trials are done for Sapthami. It takes large time to fabricate the vehicle due to limited availability of CNC machining facility. Therefore, a new tailless, wing-fuselage configuration, which can be fabricated with balsa wood, is designed. Sapthami-flyer is the second vehicle designed in this thesis. Its wing span is slightly more than Sapthami. Since it is a wing-fuselage configuration, therefore there is no need for a thick airfoil. Mark drela’s AG airfoils are found to have better lift than MH airfoils for the inverse Zimmerman profile. The thickness of the airfoils is reduced to 1% so that the wing can be made by a 1.5mm thick balsa sheet to reduce weight. The inverse Zimmermann profile wing with the AG09 airfoil is found to have best lift-to-drag ratio when compared to AG36, MH45 and MH18. The analysis is done using commercially available AVL software. AG09 with 1% thickness is used in the final configuration. This configuration has better short period damping than Sapthami. It also has slower modes of operation than Sapthami. The operating modes of most of the MAVs, including Sapthami and Sapthami-flyer, are lowly damped but fast. This makes it difficult for the pilot to fly the vehicle. To improve the flying qualities of the vehicle artificial stabilization is required. The feedback is implemented on the Kestrel autopilot hardware. It allows only PID based feedback structures to be implemented, hence gives no choice to the designer to implement higher order control. The digital integrator and differentiator implementation for feedback are non-ideal. This further reduces the effectiveness of control. The problem is dealt with by incorporating the additional dynamics introduced by these implementation while formulating the control problem. Further the modeling of the micro air vehicle is done by using vortex lattice simulation based softwares. The fidelity of the obtained dynamics is low. Therefore, there is high uncertainty in the plant model. The controller also needs to reject the wind gust disturbances which are of the order of the flight speed of the vehicle. All the above stated requirements from the control design can be best addressed by a robust control design. Sapthami-flyer uses aileron and elevator for control. There is no rudder in the configuration in order to reduce weight. In the longitudinal dynamics, pitch rate and pitch error feedback to elevator are used to increase the short period and phugoid damping respectively. In the lateral dynamics, a combination of roll rate, yaw rate and roll error feedback is given to aileron to improve the dutch roll damping and stabilize the spiral mode. The feedback loops for both longitudinal and lateral dynamics are multi-output single input design problems, therefore simultaneous tuning of loops is beneficial. The PID control is designed by first converting the actual plant to a static output feedback equivalent plant. The dynamics introduced by non-ideal differentiator and integrator implementation on the autopilot hardware are incorporated in the open loop static output feedback formulation. The robust pole placement for the SOF plant is done by using the modified iterative matrix inequality algorithm developed in this thesis. It is capable of multi-loop, multi-objective feedback design for SOF plants. The algorithm finds the optimal solution by simultaneously putting constraints on the H2 performance, pole placement, gain and phase margin of the closed loop system. The pole placement is done to minimize the real part of largest eigenvalue. A single controller is designed at a suit-able operating point and constraints are put on the gain and phase margin of the closed loop plant at other operating points. The designed controller is tested in flight on board Sapthami-flyer. The vehicle is also capable of tracking commanded pitch and roll attitude with the help of pitch error, roller or feedbacks. This is shown in the flight when the pilot leaves the RC stick and the vehicle tracks the desired attitude. The vehicle has shown improved flying characteristics in the closed loop mode.

Les essais en environnement libre et en soufflerie ont été effectués pour étudier la performance propulsive et la déformation de pales de référence et de pales souples. La poussée et le couple ont été évalués par deux méthodes: une mesure directe par balance et une estimation indirecte par bilan de quantité de mouvement, les deux méthodes ayant leurs avantages et limitations respectifs. La méthode indirecte s’est construite sur l’acquisition de champs de vitesse obtenus par PIV et s’appuie sur une estimation de la pression par mise en œuvre de l’équation de Poisson. En vol stationnaire, les pales flexibles ne peuvent pas aider à l’amélioration du rendement en mode rotor (FM), à chargement faible, puisque la distribution de vrillage est sans doute assez éloignée de l’optimal de vol stationnaire. En vol avancé, le rendement propulsif des pales flexibles est la plupart du temps plus élevé que l’hélice rigide de référence en raison de la torsion bénéfique généré en rotation. Dans le cas des pales flexibles, la vitesse axiale se trouve être inférieure au cas rigide, à même station aval; ceci correspondant à la la déformation de vrillage négatif. Pour les deux pales, la différence de poussée entre celle déduite du champ PIV test 2et celle obtenue avec la balance est plus grande que la différence entre les valeurs déduites du champ PIV test 1 et de la mesure directe. La technique de mesure laser pour les déplacements(LDS) a été utilisée pour mesurer la déformation stationnaire des pales lors de leur rotation. Par analyse du nuage de points mesurés par la LDS, la flexion et la torsion de la lame en rotation ont été identifiées à l’aide des régressions multiples. / The wind tunnel tests were conducted to explore the performance difference caused by the potential twist deformation between baseline blades and flexible blades. The balance was built in SaBre wind tunnel for measuring the thrust and torque of blades. The BEMT predictions of blades with varied twist were also performed in hover and forward flight, respectively. In hover,flexible blades cannot help in improving the FM at light disk loading since the twist generated on flexible blades is probably beyond the ideal hover twist. In forward flight, the propulsive efficiency η of flexible blades is mostly higher than baseline blades due to the beneficial twist generated in rotation. A Particle Image Velocimetry (PIV) approach of loads determination was developed based on control volume method to obtain thrust and torque of small-scale proprotor,especially for off-optimum conditions. The pressure Poisson equation was implemented for the pressure estimation based on the PIV velocity data. The axial velocity of flexible blades is found to be lower than baseline blades on the same station at downstream. This corresponds to the lower inflow ratio distribution along flexible blade, which results from the negative twist deformation. For both baseline blades and flexible blades, the thrust differences between PIV test 2 and balance are larger when compared to the differences between PIV test 1 based on nearfield and balance. The Laser Displacement Sensor (LDS) technique was employed for measuring the stationary deformation of rotating flexible blades. By obtaining the LDS point cloud, the bending and torsion of the rotating blade were identified using the multiple regressions.