The Internet of Things (IoT) has the vision to interconnect everything of the physical world and the virtual world. Advanced automated and adaptive connectivity of objects, systems, and services is expected to be achieved under the IoT context, especially in the industrial environment. Industry 4.0 with the goal of intelligent and self-adaptable manufacturing is driven by the IoT. The Object Layer, where real-time and reliable information acquisition from the physical objects carried out, is the basic enabler in the 3-layer industrial IoT system. Such acquisition system features deterministic access, reliable communication with failure resistance mechanism, latency-aware real-time response, deployable structure/protocol, and adaptive performance on various QoS demands. This thesis proposes a reliable RFID communication system for acquisition in the industrial environment. A discrete gateway structure and a contention-free communication protocol are designed to fulfill the unique system requirements. Such gateway structure offers a flexible configuration of readers and RF technologies. It enables a full duplex communication between the objects and the gateway. The designed MF-TDMA protocol can enhance the failure resistance and emergency report mechanism thanks to the separation of control link and data link in the gateway. Specifically, an optional ARQ mechanism, an independent/uniform synchronization and control method, and a slot allocation optimization algorithm are designed besides time-division and frequency-division multiplexing. Protocol implementations for different industrial situations illustrate the system ability for supporting the demands of various QoS. Finally, a 2.4-GHz/UWB hybrid positioning platform is explored based on the introduced RFID system. Taking advantage of the UWB technology, the positioning platform can achieve positioning accuracy from meter level to centimeter level. Hybrid tag prototype and specific communication process based on the MF-TDMA protocol are designed. An SDR UWB reader network, capable of evaluating multiple algorithms, is built to realize accurate positioning with an improved algorithm proposed. / <p>QC 20161109</p>

This graduation thesis is aimed to identify the current situation of the newspapers Mladá fronta DNES and the internet portal iDNES.cz in the Czech media market. The print market, as well as the Internet market is undergoing some changes mainly caused by technological progress and society development. Both products are under control from the Mafra Media Group, which has to respond to the ongoing development of the Internet, as a dynamically growing media type at the expense of newspapers. The success is the close cooperation between both journal channels and frequent use of the possibilities of promotion in other media under the Mafra Media Group.

(no abstract available) / Series: Working Papers on Information Systems, Information Business and Operations

This thesis describes the development, modification and refinement of a high-powered hybrid Stratospheric Tropospheric (ST)/meteor radar at the University of Adelaide’s Buckland Park (BP) field station. This thesis also describes the process of statistically comparing results obtained from multiple co-located independent measurement sources. Also included are statistical comparisons made between meteor radars at BP,Darwin, Northern Territory, and Davis Station, Antarctica, with other independent sources of measurement. Previous meteor radar systems have generally been low powered (∼8 kW peak) and as such could only afford low count rates at frequencies of the order of 50 MHz. While it has been shown that the echo detection rate is inversely proportional to frequency to the power of 1.5, the use of lower VHF frequencies within Australia is restricted by government regulations. As such, this has lead to the development of a high powered meteor radar system at 55 MHz which has served to facilitate higher echo rates at this frequency. The aim of improving the echo rate is to improve the statistical accuracy of results generated by the meteor technique. Also presented are descriptions of the meteor radar systems used to provide the data for this study and the basic principles of the meteor technique. Basic descriptions of the other systems and the techniques used to provide data for comparison are also presented. Two key components in the development of the high-powered meteor system are the high-powered all-sky crossed-dipole transmit antenna and the high-powered 1:2 splitter-combiner required to drive the antenna. The antenna was designed using standard equations for Yagi-Uda antenna design found in literature and modeled using the EZNEC modeling programe. After successful modeling, the antenna was prototyped and refined into a low powered version to investigate the antenna’s performance characteristics. Once the performance of the antenna was verified, the process of upgrading the antenna to handle the full output power from a VTX transmitter was performed. This upgrade also spawned the design and development of the highpowered 1:2 splitter-combiner which would be used to feed the high-powered version of the antenna. The successful operation of the high-powered system over several periods of observation has allowed for a more in-depth investigation into the statistical reliability of the meteor technique. Along with the comparison of standard atmospheric parameters, i.e. temperatures and wind velocity, the high-powered system has allowed for the verification of the relationship between echo rate and radar parameters found by McKinley, which is frequently referred to in many papers dealing with meteor observations. Along with the comparisons made with the results from the high-powered meteor radar system at BP, comparisons of atmospheric parameters derived from meteor observations and other techniques were made at Davis Station and Darwin. Of particular interest is the unique comparison of atmospheric winds made at Davis between two independent meteor radar systems and a Medium Frequency (MF) radar. Previous comparison studies have only enjoyed the benefit of having two independent sources of measurement to compare and as such have not allowed for a unique solution to be obtained for the uncertainties of the techniques using the method of Hocking et al. [2001]. Davis Station is unique in that it has two independent meteor radars in addition to a MF radar. This has enabled for the reduction in the number of degrees of freedom in the statistical comparison process, and as such has allowed for unique solutions to be determined for the uncertainties when comparing two independent techniques; i.e. meteor and MF wind comparisons. Atmospheric temperatures in the Mesospheric and Lower Thermospheric (MLT) region were determined through the use of meteor diffusion coefficients and derived atmospheric pressure models at Davis Station, BP and Darwin. Comparisons are made between the meteor technique and other co-located independent measurements. These include; airglow, satellite and falling sphere measurements at Davis Station, airglow and two independent satellite measurements at BP and two independent satellite observations at Darwin. This thesis as a whole demonstrates the successful operation of the highpowered ST/meteor hybrid radar at BP. It also demonstrates the successful comparisons of MLT winds and temperatures made between meteor radar and other independent sources of MLT measurements. The validation of using the high-powered meteor radar at BP coupled with the successful comparison of atmospheric parameters derived using the meteor technique and other forms of MLT observations serves to re-affirm the statistical accuracy and benefit of the meteor technique in observations of the MLT region. / http://proxy.library.adelaide.edu.au/login?url= http://library.adelaide.edu.au/cgi-bin/Pwebrecon.cgi?BBID=1474902 / Thesis (Ph.D.) -- University of Adelaide, School of Chemistry and Physics, 2010

There seems to be growing amount of doubts about the independance of Czech media and its position in the public space since the mid 2013, when Andrej Babiš - the second wealthiest Czech entrepreneur and since 2014 also the minister of finance -- has bought MAFRA media house, which issues among others two Czech daily papers Mladá fronta DNES and Lidové noviny. Aim of this thesis is to find out, how the change in the ownership of the MAFRA media house affected the content of the two daily papers. Such effects are analysed in articles published in Mladá fronta DNES and Lidové noviny, firstly in general in the foreign policy topics, secondly in representation of the name of Andrej Babiš and thirdly in representation of the distinct subsidiaries of the Agrofert group. Data collection is divided into two separate periods -- before and after the acquisition of MAFRA media house by Andrej Babiš. Quantitative content analysis has been chosen as the method of research.

The aim of this master thesis is to suggest the marketing plan for paid digital version of daily newspaper MF DNES. The thesis is divided into two parts -- theoretical and practical. The first part focuses on marketing plan from the theoretical point of view, where the plan and all its parts are summarized and defined into details. In the practical part, the plan is presented on the example of paid digital version of MF DNES. On the basis of deep market analysis, consumer analysis and executed marketing research, the marketing goals of the company are set. Based on these goals, the marketing strategy is suggested. The strategy is further explained in the action plan. Finally, the budget corresponding to these actions is proposed.

Anthropogenically generated antibiotic resistance genes (ARGs) are considered emerging contaminants, as they are associated with a critical human health challenge, are persist independent of a bacterial host, are subject to transfer between bacteria, and are present at amplified levels in human-impacted environments. Given the gravity of the problem, there is growing interest in advancing water treatment processes capable of limiting ARG dissemination. This study examined the potential for membrane treatment of microconstituent ARGs, and the effect of wastewater colloids on their removal. Native and spiked extracellular vanA (vancomycin resistance) and blaTEM (β-lactam resistance) ARGs were tracked by quantitative polymerase chain reaction through a cascade of membrane filtration steps. To gain insight into potential associations occurring between ARGs and colloidal material, the wastewater colloids were characterized by scanning electron microscopy, as well as in their protein, polysaccharide, and total organic carbon content. The results suggest that extracellular DNA (eDNA) containing ARGs interacts with wastewater colloids, and can both be protected against degradation and be removed more efficiently in the presence of wastewater colloidal material. Thus, ARG removal may be achievable in sustainable water reuse scenarios using lower cost membranes than would have been selected based on molecular size alone. As membranes are likely to play a vital role in water sustainability, the results of this study enable consideration of ARG removal as part of a comprehensive strategy to manage emerging contaminants and to minimize overall public health risks. / Master of Science

In eukaryotischen Zellen sind die Lipidspezies häufig asymmetrisch zwischen den Hälften der Plasmamembran verteilt. Insbesondere Phosphatidylserin (PS) weist oft eine ausgeprägte transversale Asymmetrie auf, da es fast ausschliesslich auf die innere Hälfte der Plasmamembran beschränkt ist. In den letzten Jahren wurden mehrere Proteine diskutiert, die Lipide zwischen den Membranhälften transportieren und möglicherweise die transversale Lipidasymmetrie sowie damit verbundene Zelleigenschaften beeinflussen. Im Mittelpunkt der vorliegenden Promotion steht der Auswärtstransport fluoreszierender (C6-NBD-) Lipid-Analoga und endogener Lipide durch das Multidrug Resistance 1 P-Glycoprotein (MDR1 Pgp), das der ATP Binding Cassette (ABC) Transporter Superfamilie angehört. Interessanter Weise wird für MDR1 Pgp eine ungewöhnlich breite Substratspezifität angenommen. Das anionische Lipid PS war hier von besonderem Interesse, obgleich es in vorhergehenden Arbeiten nicht als MDR1 Pgp Substrat betrachtet wurde. Der Auswärtstransport von Phosphatidylcholin-, Phosphatidylethanolamin-, Glucosylceramid- und Sphingomyelin-Analoga durch MDR1 Pgp konnte in einer humanen Magenkarzinomlinie (EPG85-257), die MDR1 überexprimiert, mittels Fluoreszenzspektroskopie bestätigt werden. Zudem legt die verringerte Akkumulation von Diacylglycerol- und Ceramid-Analoga den Transport dieser Lipidspezies durch MDR1 Pgp nahe. Im Anschluß an die intrazelluläre Markierung mit C6-NBD-PS mittels eines neuen Verfahrens konnte der signifikant erhöhte Auswärtstransport dieses Analogons in MDR1 überexprimierenden Zellen durch Verwendung spezifischer Inhibitoren MDR1 Pgp zugeschrieben werden. In flusscytometrischen Versuchen war die Exponierung von endogenem PS auf der äusseren Membranhälfte von MDR1 überexprimierenden Zellen signifikant höher als in Kontrollzellen. Verringerung der PS-Exponierung durch einen Inhibitor von MDR1 Pgp deutet auf den Transport von endogenem PS durch MDR1 Pgp hin. Zusätzlich wurde hier der Transport von C6-NBD-PS in vier weiteren Zellinien mit verschiedener Spezies- und Gewebezugehörigkeit charakterisiert, die unterschiedliche Mengen an MDR1 Pgp synthetisieren. Wie Experimente in einer BCRP überexprimierenden EPG85-257-Sublinie nahelegen, ist ausser MDR1 Pgp möglicherweise ebenfalls der ABC Halb-Transporter Breast Cancer Resistance Protein (BCRP) am Transport von C6-NBD-PS und an der verstärkten Exponierung von endogenem PS beteiligt. / In eukaryotic cells, the lipid species are frequently distributed asymmetrically between the plasma membrane leaflets. Phosphatidylserine (PS), in particular, often exhibits a distinct transverse asymmetry, being restricted almost exclusively to the inner leaflet. In the past years, several proteins were suggested to transport lipids between the leaflets of a membrane, and to potentially influence transverse lipid asymmetry and related cell properties. This thesis focuses on outward transport of fluorescent (C6-NBD-) lipid analogs and endogenous lipids by the Multidrug Resistance 1 P-Glycoprotein (MDR1 Pgp), a member of the ATP binding cassette (ABC) transporter superfamily. Interestingly, MDR1 Pgp has been suggested to exhibit an unusually broad substrate specificity. Here, the anionic PS was of particular concern, although previously reported not to be an MDR1 Pgp substrate. In a human gastric carcinoma cell line (EPG85-257) overexpressing MDR1, outward transport of phosphatidylcholine, phosphatidylethanolamine, glucosylceramide and sphingomyelin analogs via MDR1 Pgp was confirmed using fluorescence spectroscopy. In addition, decreased accumulation of analogs of diacylglycerol and ceramide suggest MDR1 Pgp mediated transport of these lipid species. Upon intracellular labelling with C6-NBD-PS using a novel approach, significantly increased outward transport of this analog in MDR1 overexpressing cells could be attributed to MDR1 Pgp by employing specific inhibitors. In a flow cytometry setup, the exposure of endogenous PS on the outer plasma membrane leaflet was significantly elevated in MDR1 overexpressing cells compared to controls. Reduction of PS exposure by an MDR1 Pgp inhibitor suggests transport of endogenous PS by MDR1 Pgp. Transport of C6-NBD-PS was furthermore characterized here in four additional cell lines of different species and tissue origin with varying synthesis levels of MDR1 Pgp. Besides MDR1 Pgp, the ABC half-size transporter Breast Cancer Resistance Protein (BCRP) is possibly also involved in transport of C6-NBD-PS and in increased exposure of endogenous PS, as found in a BCRP overexpressing EPG85-257 subline.

This diploma thesis is a part of HUMMER project. The project deals with three student’s development of two-wheeled unstable vehicle Segway type and its diminished laboratory model. This thesis deals with reviviscence of laboratory model, design of its control and realization of its actuating (a more detailed breakdown of tasks in the project is shown below). At the beginning of the work is presented reviewed study. The first part of study deals with similar models in the world, their construction and way of control. In the next part of reviewed study follows description of a real model and derivation of model basic equations, in the last part of search is given principle of operation of some used sensors. The next step was the selection and design of required electronics. In this capture are described all designed electronic modules and used sensors. There are also given parameters of used batteries and motors. The next task was the estimation of system parameters. The estimation was made by sections, in the capture is in detail described way of measuring data and structuring of estimation model. The penultimate step was design of PID and LQR controller using I/O card MF 624 and their comparing. Following thing was choice of better regulator and its implementation to the microprocessor. The last step was the realization of actuating driving of model by joystick and supreme PC.

This diploma thesis focuses on conception of values in Czech dailies after 1989. Its main goal is to picture some trends and changes in the area of values in the last twenty years. Theoretical part of the paper describes different approaches to the research of values and related concepts. Due to the extent of topic, theories and reseaches of several scientific disciplines are used. Social constructivism represents the second pillar of theoretical background because of its exploration of the influence of media on society. The original research consists in the quantitative content analysis. Based on selected value theory, list of values is used as keywords for content analysis method. Results show some trends among observed periods in the last twenty years.