Made available in DSpace on 2016-08-12T18:48:39Z (GMT). No. of bitstreams: 0 Previous issue date: 2015-02-13. Added 1 bitstream(s) on 2016-08-12T18:50:52Z : No. of bitstreams: 1 000865096.pdf: 2377110 bytes, checksum: 95208d256035a87a59c076a999bbf491 (MD5) / Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) / Introdução: Durante o envelhecimento, a perda de massa muscular (sarcopenia) é inevitável e contribui para a elevada morbidade e mortalidade; assim, evitar a sarcopenia é importante para manutenção da saúde e qualidade de vida a longo prazo. Além disso, o treinamento físico tem sido demonstrado ser importante para o controle de massa muscular durante o envelhecimento através da ativação de várias vias incluindo, IGF1-AKT e PGC-1α. Além disso, foi demonstrado que LRP130, um componente do complexo do PGC-1α é importante para a transcrição dependente de vários genes mitocondriais in vivo. Portanto, nós hipotetizamos que o treinamento físico pode melhorar a recuperação muscular, possivelmente através do complexo PGC-1α/LRP130 por inibição do sistema ubiquitina-proteassoma após atrofia induzida por desuso em ratos idosos. Portanto, foi investigado os efeitos do processo de regeneração muscular após curto período de imobilização seguido de 3 ou 7 dias de recuperação utilizando treinamento aeróbio (AT) ou treinamento resistido (TR). Métodos: Após 7 dias de imobilização, os animais foram submetidos ao processo de recuperação muscular com exercício (AT ou RT) ou sem exercício (R) durante 3 ou 7 dias. No final do experimento, os animais foram sacrificados, e o músculo plantar (PL) foi removido para análises morfológicas (avaliação da atividade mATPase, para avaliar a área de secção transversal das fibras musculares (CSA), bem como a frequência dos tipos de fibras específicas), análises bioquímicas (isoformas da cadeia pesada de miosina, MyHC), expressão gênica de 44 marcadores moleculares de massa muscular (RT-qPCR). Para entender melhor a relevância biológica das interações de mRNA, foi realizada uma análise de rede que estão relacionados com o nosso conjunto de genes diferencialmente expressos (plugin GeneMANIA Cytoscape). Os genes diferencialmente expressos foram submetidos à análise... / Background: During aging, loss of muscle mass (sarcopenia) is inevitable and contributes to increased morbidity and mortality; thus, the maintenance of skeletal muscle mass is critical for long-term health and quality of life. In addition, physical training has been shown to be important to the control of muscle mass during aging through the activation of several pathways including, IGF1-AKT and PGC-1α. Also, it was demonstrated that LRP130, a component of the PGC-1α complex is important for the PGC-1α- dependent transcription of several mitochondrial genes in vivo. Therefore, we hypothesized that the physical training could improve the muscle recovery possibly through of the LRP130/PGC-1α complex by inhibiting the ubiquitin-proteasome system after disuse-induced atrophy in aged rats. We therefore investigated the effects on muscle recovery after short-term immobilization followed by 3 or 7 days of retraining with aerobic training (AT) or resistance training (RT). Methods: After 7 days of immobilization, the animals underwent the re-growth process with exercise (AT or RT) or without exercise (R) for 3 or 7 days. At the end of the experiment, the animals were sacrificed, and the plantaris (PL) muscle was removed for morphological analyses (assessment of mATPase activity, to assess the total muscle fiber cross sectional area (CSA), and the frequency of specific fiber types), biochemical analyses (Myosin Heavy Chain, MyHC), gene expression of 44 molecular markers of muscle mass (RT-qPCR). To further understand the biological relevance of the mRNA interactions, we performed a search for additional genes that are related to our differentially expressed gene set (GeneMANIA Cytoscape plugin). Differentially expressed genes were submitted to Western blotting analysis. Results: At 7 days after an atrophic stimulus, only PL muscles from animals subjected to AT had recovered their normal CSA patterns. The frequency of fiber types and ... / FAPESP: 11/14484-4

Orientador: / Coorientador: / Banca: / Resumo: Introdução: Durante o envelhecimento, a perda de massa muscular (sarcopenia) é inevitável e contribui para a elevada morbidade e mortalidade; assim, evitar a sarcopenia é importante para manutenção da saúde e qualidade de vida a longo prazo. Além disso, o treinamento físico tem sido demonstrado ser importante para o controle de massa muscular durante o envelhecimento através da ativação de várias vias incluindo, IGF1-AKT e PGC-1α. Além disso, foi demonstrado que LRP130, um componente do complexo do PGC-1α é importante para a transcrição dependente de vários genes mitocondriais in vivo. Portanto, nós hipotetizamos que o treinamento físico pode melhorar a recuperação muscular, possivelmente através do complexo PGC-1α/LRP130 por inibição do sistema ubiquitina-proteassoma após atrofia induzida por desuso em ratos idosos. Portanto, foi investigado os efeitos do processo de regeneração muscular após curto período de imobilização seguido de 3 ou 7 dias de recuperação utilizando treinamento aeróbio (AT) ou treinamento resistido (TR). Métodos: Após 7 dias de imobilização, os animais foram submetidos ao processo de recuperação muscular com exercício (AT ou RT) ou sem exercício (R) durante 3 ou 7 dias. No final do experimento, os animais foram sacrificados, e o músculo plantar (PL) foi removido para análises morfológicas (avaliação da atividade mATPase, para avaliar a área de secção transversal das fibras musculares (CSA), bem como a frequência dos tipos de fibras específicas), análises bioquímicas (isoformas da cadeia pesada de miosina, MyHC), expressão gênica de 44 marcadores moleculares de massa muscular (RT-qPCR). Para entender melhor a relevância biológica das interações de mRNA, foi realizada uma análise de rede que estão relacionados com o nosso conjunto de genes diferencialmente expressos (plugin GeneMANIA Cytoscape). Os genes diferencialmente expressos foram submetidos à análise... / Abstract: Background: During aging, loss of muscle mass (sarcopenia) is inevitable and contributes to increased morbidity and mortality; thus, the maintenance of skeletal muscle mass is critical for long-term health and quality of life. In addition, physical training has been shown to be important to the control of muscle mass during aging through the activation of several pathways including, IGF1-AKT and PGC-1α. Also, it was demonstrated that LRP130, a component of the PGC-1α complex is important for the PGC-1α- dependent transcription of several mitochondrial genes in vivo. Therefore, we hypothesized that the physical training could improve the muscle recovery possibly through of the LRP130/PGC-1α complex by inhibiting the ubiquitin-proteasome system after disuse-induced atrophy in aged rats. We therefore investigated the effects on muscle recovery after short-term immobilization followed by 3 or 7 days of retraining with aerobic training (AT) or resistance training (RT). Methods: After 7 days of immobilization, the animals underwent the re-growth process with exercise (AT or RT) or without exercise (R) for 3 or 7 days. At the end of the experiment, the animals were sacrificed, and the plantaris (PL) muscle was removed for morphological analyses (assessment of mATPase activity, to assess the total muscle fiber cross sectional area (CSA), and the frequency of specific fiber types), biochemical analyses (Myosin Heavy Chain, MyHC), gene expression of 44 molecular markers of muscle mass (RT-qPCR). To further understand the biological relevance of the mRNA interactions, we performed a search for additional genes that are related to our differentially expressed gene set (GeneMANIA Cytoscape plugin). Differentially expressed genes were submitted to Western blotting analysis. Results: At 7 days after an atrophic stimulus, only PL muscles from animals subjected to AT had recovered their normal CSA patterns. The frequency of fiber types and ... / Doutor

O cirurgião dentista é constantemente exposto a um elevado número de doenças ocupacionais. Alterações no sistema musculoesquelético são freqüentes, principalmente nos membros superiores e coluna vertebral, que podem interferir negativamente, na capacidade funcional desses profissionais. O objetivo deste trabalho é contribuir para o entendimento das doenças ocupacionais associadas ao sistema musculoesquelético relacionado à coluna vertebral dos odontólogos, durante sua atividade laboral, bem como as questões psicossociais associadas. Este documento contempla uma sequência de dois artigos sobre as condições de trabalho e desordens musculoesqueléticas relacionadas à coluna vertebral em dentistas. O primeiro apresenta uma revisão teórica sobre a atividade laboral dos dentistas, evidenciando os problemas relacionados à coluna vertebral, à carga de trabalho, aos riscos biomecânicos, às principais patologias e fatores psicossociais associados. Desta maneira, se evidenciou que estes profissionais estão sujeitos a uma alta carga de trabalho e desenvolvendo posturas que comprometem a integridade da coluna vertebral gerando dor e incapacidade para o trabalho. O segundo apresenta um método de análise e um estudo aplicado para investigar as desordens musculoesqueléticas relacionadas à coluna vertebral e fatores associados às condições ocupacionais de dentistas. Este artigo explicita as regiões da coluna vertebral mais acometida por queixas musculoesqueléticas e fatores associados. O método investigativo proposto mostrou que os problemas concernentes ao trabalho dos dentistas estão provocando dores e alterações musculoesqueléticas na região da coluna vertebral e são devidos principalmente à postura de trabalho, o esforço físico, os movimentos repetitivos e carga horária de trabalho. / The dental surgeon is constantly exposed to a large number of occupational illnesses. Alterations in the musculoskeletal system are frequent, especially in the upper limbs and spine, which can interfere negatively in the functional capacity of these professionals. The aim of this study is to contribute to the understanding of occupational diseases associated to the musculoskeletal system. Mainly those related to the spine of dentists during their work activities as well as psychosocial issues. This document includes a sequence of two articles about the working conditions and musculoskeletal disorders related to the spine of dentists. The first one presents a theoretical review about labor activity of dentists, highlighting the problems related to the spine, the workload, the biomechanical risks, the major pathologies and psychosocial factors associated. In this way, it is evidenced that these professionals are subject to a high workload and developing postures that compromise the integrity of the spine causing pain and inability to work. The second presents a method of analysis and an applied study to investigate musculoskeletal disorders related to the spine and occupational factors associated with conditions of dentists. This article explains the spinal regions most affected by musculoskeletal complaints and associated factors. The investigative method proposed, showed that problems regarding to the work of dentists are causing pain and musculoskeletal alterations in the spinal region and are due mainly to the working posture, physical effort, repetitive movements and workload.

O cirurgião dentista é constantemente exposto a um elevado número de doenças ocupacionais. Alterações no sistema musculoesquelético são freqüentes, principalmente nos membros superiores e coluna vertebral, que podem interferir negativamente, na capacidade funcional desses profissionais. O objetivo deste trabalho é contribuir para o entendimento das doenças ocupacionais associadas ao sistema musculoesquelético relacionado à coluna vertebral dos odontólogos, durante sua atividade laboral, bem como as questões psicossociais associadas. Este documento contempla uma sequência de dois artigos sobre as condições de trabalho e desordens musculoesqueléticas relacionadas à coluna vertebral em dentistas. O primeiro apresenta uma revisão teórica sobre a atividade laboral dos dentistas, evidenciando os problemas relacionados à coluna vertebral, à carga de trabalho, aos riscos biomecânicos, às principais patologias e fatores psicossociais associados. Desta maneira, se evidenciou que estes profissionais estão sujeitos a uma alta carga de trabalho e desenvolvendo posturas que comprometem a integridade da coluna vertebral gerando dor e incapacidade para o trabalho. O segundo apresenta um método de análise e um estudo aplicado para investigar as desordens musculoesqueléticas relacionadas à coluna vertebral e fatores associados às condições ocupacionais de dentistas. Este artigo explicita as regiões da coluna vertebral mais acometida por queixas musculoesqueléticas e fatores associados. O método investigativo proposto mostrou que os problemas concernentes ao trabalho dos dentistas estão provocando dores e alterações musculoesqueléticas na região da coluna vertebral e são devidos principalmente à postura de trabalho, o esforço físico, os movimentos repetitivos e carga horária de trabalho. / The dental surgeon is constantly exposed to a large number of occupational illnesses. Alterations in the musculoskeletal system are frequent, especially in the upper limbs and spine, which can interfere negatively in the functional capacity of these professionals. The aim of this study is to contribute to the understanding of occupational diseases associated to the musculoskeletal system. Mainly those related to the spine of dentists during their work activities as well as psychosocial issues. This document includes a sequence of two articles about the working conditions and musculoskeletal disorders related to the spine of dentists. The first one presents a theoretical review about labor activity of dentists, highlighting the problems related to the spine, the workload, the biomechanical risks, the major pathologies and psychosocial factors associated. In this way, it is evidenced that these professionals are subject to a high workload and developing postures that compromise the integrity of the spine causing pain and inability to work. The second presents a method of analysis and an applied study to investigate musculoskeletal disorders related to the spine and occupational factors associated with conditions of dentists. This article explains the spinal regions most affected by musculoskeletal complaints and associated factors. The investigative method proposed, showed that problems regarding to the work of dentists are causing pain and musculoskeletal alterations in the spinal region and are due mainly to the working posture, physical effort, repetitive movements and workload.

Ce travail de thèse s’inscrit dans le cadre de la simulation par mannequins numériques de l’homme (en anglais Digital Human models ou DHM) en vue d’applications ergonomiques. Plus précisément, il s’intéresse à la modélisation du système musculosquelettique et des efforts musculaires développés au cours du mouvement.Dans un première partie est présenté le développement numérique de modèles musculosquelettiques du membre inférieur et supérieur sous l’environnement Matlab. Ces modèles ont été évalués par examen des bras de levier musculaires pour la géométrie, et par comparaison avec des mesures expérimentales de couples articulaires maximaux (FMV) pour les efforts prédits.Dans une seconde partie, le modèle musculosquelettique du membre inférieur a été utilisé pour évaluer le rôle des muscles bi-articulaires au cours de la marche. Pour cela deux méthodes de calcul des efforts musculaires ont été utilisées, permettant de mettre en évidence les particularités fonctionnelles de ces muscles. Les résultats indiquent notamment que la prise en compte des muscles bi-articulaires est nécessaire à une estimation correcte des efforts musculaires et des efforts de contact articulaires, potentielles sources d’inconfort.Puis ont été étudiés les capacités d’effort maximale en flexion-extension isométrique du coude. L’expérimentation a été effectuée au sein de l’INRETS de Bron et comprenait 9 sujets volontaires masculins. Cette étude a permis d’évaluer le modèle numérique développé en termes de prédiction de capacités d’effort, ainsi que d’ouvrir la voie à des méthodes de scaling permettant d’ajuster un modèle musculosquelettique au sujet étudié. / This work deals with the use of Digital Human models (DHM) for ergonomic applications. More precisely, it focuses on the modelling of the musculoskeletal system and muscular forces developed during movement.In the first part, the development of numerical musculoskeletal models of the lower and upper limbs under the Matlab environment is presented. These models were evaluated by examination of the muscles’ lever arms (for geometry) and by experimental measurements of maximum voluntary force (MVF) (for predicted muscle forces).In a second part, the lower limb muscular model was used to evaluate the role of biarticular muscles during gait. To achieve this, two methods were used, allowing to highlight the functional specificities of these muscles. The results indicate in particular that taking into account biarticular muscles is necessary to a correct estimation of muscle forces and joint contact forces, which are potential discomfort sources.Finally, the maximal force capacity of isometric elbow flexion-extension has been studied. The goal was to set up scaling methods allowing to fit the muscular model to the studied subject’s capacities. The experiment took place at INRETS, and implicated 9 male voluntary subjects. This study allowed to evaluate the model in terms of maximal force capacity prediction, and openend the path to new scaling methods for musculoskeletal models.

Osteoarthritis develops secondary to the action of hostile biomechanics upon susceptible cartilage. Cam morphology describes a loss of concavity at the femoral head-neck junction resulting in femoral impaction against the acetabular rim within a functional range of movement. This impaction is termed femoroacetabular impingement and can result in pain and cause damage to adjacent articular cartilage that progresses to osteoarthritis. Cam morphology is a target for joint preservation strategies. The first study in this thesis compares hip development in academy football players with controls from local schools. The results provide strong evidence that cam morphology can develop in response to intense sporting activity during adolescence. At present, it is not possible to recommend activity modification as the cardiovascular benefits of exercise are likely to outweigh potentially detrimental effects on hip morphology. Nevertheless, individuals participating in high-level sports during youth may represent a high risk cohort for osteoarthritis warranting surveillance. Diagnostic tools currently available only allow identification of late joint degeneration when disease is irreversible. Advances in the field of disease biomarkers may overcome this challenge. The second study of this thesis explored the prognostic value of compositional MRI. Baseline delayed gadolinium-enhanced MRI of cartilage was able to predict the development of radiographic hip osteoarthritis in asymptomatic individuals within five years. As well as identifying individuals who are likely to benefit from intervention, compositional MRI may allow the evaluation of treatment efficacy within short timeframes. A number of interventions aimed at joint preservation are under investigation for treating cam morphology femoroacetabular impingement. Arthroscopic surgery is increasingly performed to restore the concavity at the femoral head-neck junction and prevent impaction against the acetabular rim. A feasibility study was performed for a proposed randomised controlled study comparing operative and non-operative treatment. The study protocol was developed for the 'Femoroacetabular Impingement Trial' (FAIT).

Abdominal pain of non-visceral origin has been recognised as a clinical entity for many years. In many gastroenterology clinics up to 50 per cent of patients attending have no pathological cause to their symptoms and such patients often become chronic attenders and suffer repeated investigation without resolution of their problem. They are often left with a label' of Irritable Bowel Syndrome (lBS) without a precise diagnosis being made. This is both unsatisfactory for the patient and physician. This study set out to determine the incidence of musculoskeletal causes of abdominal pain and to determine what diagnostic tools will help identify this group of patients and thus allow the physicians to refer the appropriate patients at an early stage. The aims were to identify questions that act as predictors of the presence of abdominal pain of musculoskeletal origin, patterns of pain presented in this group of patients and the ability of physiotherapists to detect cases of abdominal pain of musculoskeletal origin. The incidence of abdominal pain of musculoskeletal origin in this study was 14 per cent. Questions that act as predictors include an affirmative response to pain being aggravated by movements such as bending, twisting and turning, and coughing and sneezing, and a negative response to change in bowel habit, symptoms being aggravated by food and no weight change. The ability of the physiotherapist to detect cases was 88.3 per cent. No particular pattern of pain areas emerged to differentiate patients with abdominal pain of visceral and musculoskeletal causes. Early assessment of the musculoskeletal system by a trained physiotherapist is recommended. An early referral will lead to prompt and appropriate treatment and, consequently, to a reduction in costs for the NHS. For patients where the cause of their abdominal pain is not obvious it is unacceptable that they are left with the diagnosis of IBS without the musculoskeletal system being assessed. This study shows that such an assessment is vital to detect cases where the pain has a musculoskeletal origin.

Magister Scientiae (Physiotherapy) - MSc(Physio) / Lack of adherence to the correct working conditions exposes workers to ergonomics-related hazards and eventually work-related musculoskeletal disorders (WRMDs) which are estimated at 160 million per year globally. Literature shows that with modernization by use of computers, administrators are exposed to prolonged sitting and long working hours which predisposes them to ergonomic hazards. Low back pain and neck pain are the leading work-related musculoskeletal disorders with a lifetime prevalence of 70 - 80% and 50 - 60% respectively.Both low back pain and neck pain have a multifactorial aetiology that includes work-related and individual related factors. Lack of reporting of work-related injuries has led to paucity of statistical literature with regards to work-related low back pain and neck pain, especially in the developing countries. The aim of this study was to determine the prevalence of ergonomics related low back pain and neck pain, and describe the effect of a knowledge-based ergonomic intervention among administrators in Aga Khan University Hospital, Nairobi (AKUH, N). A mixed method design was used in this study using a survey and two focus group discussions(FGD). A self-administered questionnaire that is in four sections was administered to 208 participants. The questionnaire sought the prevalence of musculoskeletal disorders, the knowledge of participants with regards to low back pain and neck pain as well as the work-related and individual risk factors related to the same. The dissemination of the study results involved a one hour knowledge-based ergonomic session given to all interested participants, based on the information from the survey. Two FGD with purposive selection of eight participants were held to explore their experience on the value of the information provided.The statistical package for social sciences (SPSS) version 20 was used to capture and analyze the quantitative data. Descriptive statistics was used to summarize the study findings in the form of means, frequencies, standard deviations and percentages. Inferential statistics (chi-square) was used to test the associations between different categorical variables (p<0.05). For the qualitative data, the tape recorded interviews were transcribed verbatim, field notes typed, sorting and arranging data was done and themes were generated. Thematic content analysis was used to generate the themes. The aim of the study, confidentiality and the participants‘ freedom to withdraw from the study were explained. Informed consent was also obtained before the survey and FGD and referral was made where necessary. The findings of the current study revealed that the study participants were knowledgeable about ergonomics-related low back pain and neck pain however this knowledge was not directly translated into behaviour. Low back pain (LBP) had the highest twelve month prevalence at 75.5% followed by neck pain at 67.8%, and LBP showed significant associations at p< 0.05 with some work-related and individual risk factors. The results of the FGDs showed that most participants had positive behavioural and attitudinal change post the knowledge-based ergonomic intervention despite the challenges they met in implementing the behavioural change. These results therefore show the need for continual education about ergonomics to create awareness on the predisposing factors to work-related LBP and neck pain, hence promoting a healthier quality of life amongst employees through adherence to healthy work behavioural practice.

Background: Literature has associated repeated eccentric muscle actions with increased muscle damage of the muscles involved. Eccentric actions are typical in sports which are ‘stop-start’ in nature requiring rapid acceleration and deceleration, typical of a batting activity in cricket. Ultra-structural damage of the skeletal muscle as a consequence of repeated decelerating activities is associated with performance decrements, particularly muscle strength and sprinting speeds. This suggests that eccentric strength decrements may provide an indication for the development of muscle strain injuries during these activities. Despite these findings, limited research has identified the specific musculoskeletal demands placed on cricket batsmen, particularly with reference to various match intensities. Objective: The present study, therefore, sought to determine the specific musculoskeletal, physiological and perceptual demands placed on specialised batsmen during two work bouts of different intensities; one representing a highintensity work bout and the other a moderate-intensity work bout. The dependent variables of interest were muscle activation, isokinetic strength changes, heart rate, ‘central’ and ‘local’ ratings of perceived exertion (RPE), body discomfort and performance. Methods: The two experimental conditions, representative of a high- (HVR) and moderate-volume running (MVR) batting protocol, required players to perform a simulated batting work bout of either twelve or six runs an over, within a laboratory setting. Selected physiological, perceptual and performance measures were collected at specific time intervals throughout the work bout while the biophysical measures were collected prior to, and following both protocols. Results: Of the variables measured, heart rate, ‘central’ and ‘local’ RPE values were observed to increase significantly (p<0.05) over time. This increase was greater as a consequence of the HVR in comparison to the MVR. No change in sprint times was documented during the MVR, in contrast, significant (p<0.05) increases over time were observed during the HVR, further highlighting the elevated demands associated with this condition. In addition, an ‘end spurt’ was observed particularly following the HVR condition, suggesting athletes were conserving themselves through the adoption of a pacing strategy. Reductions in biceps femoris and semitendinosus muscle activation levels were observed following the HVR. This was further supported by the significantly greater levels of semitendinosus activation following the MVR when compared to the HVR. Peak concentric and eccentric knee extensor (EXT) (-17.17% and -16.07% respectively) and eccentric flexor (FLEX) (- 17.49%) values decreased significantly (p<0.05) following the HVR at 60°.s-1. In addition, concentric and eccentric total work produced by the flexors and eccentric extensors resulted in significantly (p<0.05) lower values due to the HVR. Conclusion: The intermittent high-volume batting work bout elicited elevated mean heart rates, perceived ratings of cardiovascular and muscular effort and sprint times. Furthermore, hamstring activation levels and muscle strength, particularly concentric strength of the dominant lower limb were negatively affected by the HVR condition. These results suggest elevated demands were placed on the hamstring musculature as a consequence of the HVR condition, indicating a greater degree of musculoskeletal strain and increased injury risk associated with running between the wickets at this intensity, representative of an aggressive batting scenario.

We describe a semi-automatic technique for modeling and animating complex musculoskeletal systems using a strand based muscle model. Using our interactive tools, we are able to generate the motion of tendons and muscles under the skin of a traditionally animated character. This is achieved by integrating the traditional animation pipeline with a biomechanical simulator capable of dynamic simulation with complex routing constraints on muscles and tendons. We integrate our musculoskeletal modeling and animation toolkit into a professional 3D production environment, thereby enabling artists and scientists to create complex musculoskeletal systems that were previously inaccessible to them. We demonstrate the applications of our tools to the visual effects industry with several animations of the human hand and applications to the biomechanics community with a novel model of the human shoulder. / Science, Faculty of / Computer Science, Department of / Graduate