This thesis is an investigation of photoperiodic injury (PI) in tomato plants and practices to alleviate the problem. PI is a physiological disorder characterized by chlorosis and necrosis of leaves when plants are grown under either long photoperiods or non-24 h light/ dark cycles. Solanum lycopersicum L. (tomato) is particularly susceptible to PI. Our group has shown a correlation between PI and altered circadian expression patterns for the nitrate assimilatory enzymes nitrate reductase (NR) and nitrite reductase (NiR) in tomato, resulting in accumulation of the toxic metabolite nitrite, particularly at specific times of day (TOD) when the NiR/NR activity ratio is low. We hypothesized that accumulation of nitrite and PI can be alleviated by altering nitrate nutrition at specific times of day and the use of an air temperature differential. The tomato cultivars ‘Micro Tom’ (PI-tolerant) and ‘Basket Vee’ (PI-susceptible) were grown under various photoperiod regimes to determine: (1) if a positive correlation exists between PI and nitrite accumulation as determined by visual assessment, and chlorophyll and nitrite quantification; (2) if 24 h light affects the diel pattern of nitrate uptake in a way that favours PI through measurements of nitrate depletion; and (3) if PI can be alleviated by altering nitrate nutrition at two specific TOD when tomato is susceptible to nitrite accumulation. A positive correlation was found between nitrite accumulation and PI. Nitrate uptake experiments showed that the nitrate uptake rate per se is not responsible for PI in tomato, but maintenance of circadian nitrate uptake patterns even in 24 h light in combination with a loss of the circadian patterns for NR and NiR activities could contribute to PI. Nitrite accumulation and PI was decreased by utilizing a nutrient solution containing 25% total nitrogen at two specific 4 h periods in the day when tomato is susceptible to nitrite accumulation. We call this new technique TOD fertigation. Time-of-day fertigation in combination with a 6 oC temperature differential further reduced nitrite accumulation and PI. These findings showed the response of greenhouse tomatoes to supplemental lighting and the potential for increasing the photoperiod threshold for PI. / The Mexican Council for Science and Technology (CONACYT); The Ontario Ministry of Agriculture, Food and Rural Affairs (OMAFRA)

Nitrogen is required by plants in large quantities and its deficiency is mostly related to reduction in crop production. A study was conducted to assess the importance of nitrogen in tomato (Lycopersicon esculentum Mill.) transplant production. Transplants were propagated at 0, 30, 60, 90, and 120 mg∙L-1 N applied as NH4NO3 while 30 mg∙L-1 P applied as NaH2PO4 and 30 mg∙L-1 K as KCl were used. Fergitation was done by floating cavity trays in nutrient solution until the medium reached field capacity. The experiment was arranged in a randomized complete block design (RCBD) with four replications. Sampling was initiated at 21 days after sowing and was done weekly until the transplants were ready for transplanting (when transplants could be pulled out of the cavity easily without breaking) at 42 days after sowing. Nitrogen supply had a pronounce influence on the transplant root and shoot growth. Observations throughout the experiment indicated that increased nitrogen application favoured shoot growth which is an indication that most of the assimilates were partitioned to shoots rather than to roots. Nitrogen application of 120 mg∙L-1increased fresh shoot mass and subsequently enhanced dry shoot mass. As nitrogen was increased from 0 to 120 mg∙L-1, it further promoted relative growth rate, specific leaf area, leaf mass ratio, leaf area ratio, plant chlorophyll content, leaf tissue nitrogen and improved the pulling success. At 42 days after sowing, a quality transplant that was produced with 90 mg∙L-1 N, had a root to shoot ratio of 0.16, leaf mass ratio of 0.86, root mass ratio of 0.13, leaf area of 594 cm2, plant chlorophyll content of 33, leaf tissue nitrogen of 32 g∙kg-1, specific leaf area of 194 cm2∙mg-1, leaf area ratio of 167.7 cm2∙mg-1, relative growth ratio of 0.31 cm∙mg-1∙wk-1 and 100% pulling success. This transplant proved to be ideal for the production of tomato as compared to other treatment combinations that were employed. Another glasshouse experiment was conducted to determine the influence of electrical conductivity (EC) and or nutrient solution composition on growth, yield and quality parameters in tomato. The pots were arranged in a randomized complete block design (RCBD). One plant per pot represented an experimental unit. Four EC treatments were used that consisted of 1.12, 2.24, 4.48 and 6.72 mS∙cm-1. Each treatment was replicated six times. Distilled water was used for irrigation water to maintain the required pH, which was 5.5 to 6.2 throughout the duration of the study, and cocopeat was used as substrate. Salinity inhibited growth (shoot length) and yield (average fruit mass, fruit diameter and fruit circumference) at the highest concentration of 6.72 mS∙cm-1. However, it did not significantly affect number of trusses, number of fruits and stem diameter, rather tomato quality was improved in terms of total soluble solids. Although tomato fruits grown at 6.72 mS∙cm-1 were relatively smaller than fruits grown at 1.12, 2.24 and 4.48 mS∙cm-1 treatments respectively, they had higher acidity, increased soluble solids and higher sugar content which are all qualities required by the tomato processing industry. Increasing the concentration of the solution from 1.12 to 6.72 mS∙cm-1 increased the %Brix from 3.9 to 6.1% while titratable acidity was also increased from 3.3 to 5.7%, respectively. The incidents of blossom end rot were higher (6.3%) at concentration of 6.72 mS∙cm-1 as compared to 1.12 mS∙cm-1 concentration, which was 0.5%. / Dissertation (MScAgric)--University of Pretoria, 2014. / tm2015 / Plant Production and Soil Science / MScAgric / Unrestricted

A utilização do capim-Tanzânia tem sido crescente nas pastagens brasileiras e há nítida demanda de maior conhecimento sobre as necessidades nutricionais dessa forrageira, com o intuito de se obter mais altas produtividades. O objetivo desta pesquisa foi de obter informações desse capim no que diz respeito à relação entre os nutrientes potássio e magnésio, através da avaliação do número de perfilhos e de folhas, área foliar, produção de massa da parte aérea e das raízes, superfície e comprimento radicular, leitura SPAD, teor de clorofila nas folhas e concentrações dos macronutrientes catiônicos nos tecidos das plantas e caracterização de sintomas visuais. Foi conduzido um experimento em casa-de-vegetação, com solução nutritiva, em vasos de 3,6 L tendo sílica moída como substrato, no período de setembro a novembro de 2004, em Piracicaba-SP. Utilizou-se o esquema fatorial 52 incompleto, perfazendo um total de 13 combinações de doses de potássio e de magnésio, em mmol L-1: 0,4K/0,05Mg; 0,4K/1,35Mg; 0,4K/2,65Mg; 3,2K/0,7Mg; 3,2K/2Mg; 6K/0,05Mg; 6K/1,35Mg; 6K/2,65Mg; 8,8K/0,7 Mg; 8,8K/2Mg; 11,6K/0,05Mg; 11,6K/1,35Mg; 11,6K/2,65Mg, em delineamento experimental de blocos ao acaso, com quatro repetições. O primeiro corte das plantas foi realizado 46 dias após o transplante das mudas e o segundo 28 dias após o primeiro. Em ambos os cortes a parte aérea das plantas foi separada em folhas emergentes, lâminas de folhas recém-expandidas, lâminas de folhas maduras e colmos mais bainhas. Após o segundo corte realizou-se também a separação das raízes, utilizando-se água corrente e peneiras de 0,25 e 1,00 mm. As combinações de doses de potássio e de magnésio foram determinantes para a proporção de potássio, magnésio e cálcio na parte aérea nos dois períodos de crescimento, para comprimento e massa radicular e, somente no segundo crescimento, para produção da parte aérea e para as concentrações de cálcio e de manganês nas lâminas de folhas recém-expandidas do capim. De forma isolada as doses de potássio alteraram o número de perfilhos e de folhas, a área foliar e a concentração de zinco nas lâminas de folhas recém-expandidas nos dois crescimentos, enquanto influenciaram, no primeiro crescimento, a produção da parte aérea e as concentrações de cálcio, cobre, ferro e manganês nas folhas diagnósticas, além de promover alteração na superfície radicular. As doses de magnésio, isoladamente, fizeram variar o valor SPAD e a concentração de clorofila nos dois crescimentos, e no segundo crescimento, têm influência na área foliar, na superfície radicular e nas concentrações de cobre, ferro e zinco nas lâminas de folhas recém-expandidas da gramínea. As máximas respostas do capim-Tanzânia ocorreram com fornecimento de potássio na solução nutritiva entre 8,4 e 10,9 mmol L-1 e o de magnésio entre 1,7 e 2,3 mmol L-1, e com participação do potássio entre 53 e 64 % e do magnésio ao redor de 20 % na concentração total de potássio, magnésio e cálcio na parte aérea das plantas. Sintomas visuais das deficiências de potássio e de magnésio nas plantas somente foram observados com baixos suprimentos desses nutrientes. / Tanzânia grass use in Brazilian pastures has been improved, and there is a need for more knowledge on its mineral nutrition in order to increase forage productivity. The objective of this research was to obtain information for this grass related to the combined supply of potassium and magnesium, through the evaluation of number of tillers and leaves, leaf area, plant tops and roots dry matter production, roots lenght and surface, SPAD value, leaf chlorophyll concentration, cationic macronutrients concentrations in plant tissues and visual symptoms characterization. An experiment was carried out in a greenhouse by using nutrient solution in 3.6 L pots containing ground quartz, from September to November 2004, at Piracicaba, São Paulo State, Brasil. An incomplete 52 factorial was used, which resulted in 13 combinations among potassium and magnesium rates, in mmol L-1: 0,4K/0,05Mg; 0,4K/1,35Mg; 0,4K/2,65Mg; 3,2K/0,7Mg; 3,2K/2Mg; 6K/0,05Mg; 6K/1,35Mg; 6K/2,65Mg; 8,8K/0,7 Mg; 8,8K/2Mg; 11,6K/0,05Mg; 11,6K/1,35Mg; 11,6K/2,65Mg, in a randomized block design, with four replications. Plants were first harvested 46 days after seedlings transplanting to the pots, and harvested again 28 days after the first harvest. At each harvest plants tops were separated into emergent leaves, newly expanded leaf laminae, mature leaf laminae and culms plus sheaths. After the second harvest the roots were collected by passing tap water through 0.25 and 1.00 mm screens. Potassium and magnesium rates combinations highly influenced the proportion of potassium, magnesium and calcium in plant tops in both growth periods, and the roots dry weight and length. These nutrients rates combinations also determined forage yield and concentrations of calcium and manganese in the newly expanded leaf laminae at the second harvest. Potassium rates significantly changed the number of tillers and leaves, leaf area, and zinc concentration in the newly expanded leaves at both growth periods, whereas in the first growth influenced forage yield, concentrations of calcium, copper, iron and manganese in the newly expanded leaf laminae, and in the root surface. Magnesium rates resulted in changes in SPAD values and chlorophyll concentrations in the two plant growths but, at second growth, promoted changes in leaf area, in root surface, and in concentrations of copper, iron and zinc in the newly expanded leaf laminae. Maximum responses of Tanzania grass were found when potassium was supplied between 8.4 and 10.9 mmol L-1 and magnesium between 1.7 and 2.3 mmol L-1 and when potassium contributed between 53 and 64 % and magnesium about 20 % to the total concentration of potassium, magnesium and calcium in plants tops. Visual symptoms of potassium and magnesium deficiencies in the plants were only observed with low rates supply of these nutrients.

Foram conduzidos dois experimentos em casa-de-vegetação com o Panicum maximum cv. Mombaça cultivado em solução nutritiva, tendo sílica como substrato. Um experimento envolveu oito doses de potássio (0; 9,75; 39; 78; 156; 234; 312 e 468 mg L-1) e o outro oito doses de magnésio ( 0; 1,2; 3,6; 12; 24; 36; 48 e 60 mg L-1), sendo ambos conduzidos no período de janeiro a março de cada ano. O objetivo foi o de estudar o efeito das doses de potássio e magnésio na produção de massa seca, no número de perfilhos, na área foliar, no teor de clorofila estimado através da leitura SPAD, na concentração e acúmulo destes nutrientes nos componentes da planta e na determinação do nível crítico. O delineamento estatístico foi de blocos completos ao acaso, com quatro repetições. Observou-se efeito significativo e positivo das doses de potássio na produção de massa seca da parte aérea e das raízes, expressado por regressão quadrática e também na área foliar, representada por efeito linear. As doses de potássio afetaram significativamente o número de perfilhos, porém não influenciaram no teor de clorofila da lâmina foliar. A concentração de potássio nas folhas emergentes, nas lâminas de folhas recém-expandidas, nas lâminas de folhas maduras, nos colmos mais bainhas e nas raízes aumentou significativamente com o incremento de potássio na solução nutritiva. O maior e menor acúmulo de potássio na massa seca da parte aérea foram observados nos colmos mais bainhas e nas lâminas de folhas maduras, respectivamente. Observou-se efeito significativo positivo das doses de magnésio na produção de massa seca da parte aérea e das raízes, na área foliar e na estimativa do teor de clorofila, sendo representados por regressões quadráticas e lineares em função da variável estudada. As doses de magnésio aumentaram o número de perfilhos até a dose de 48 mg L-1. A concentração de magnésio nas folhas emergentes, nas lâminas de folhas recém-expandidas, lâminas de folhas maduras, nos colmos mais bainhas e nas raízes aumentou significativamente com o incremento de magnésio na solução nutritiva até a máxima dose estudada. O maior e menor acúmulo de magnésio na massa seca da parte aérea foram constatados nos colmos mais bainhas e nas lâminas de folhas emergentes, respectivamente. As lâminas de folhas recém-expandidas foram consideradas indicadoras para a avaliação do estado nutricional do capim-Mombaça associadas ao nível crítico de potássio entre 15,4 e 17,3 g kg-1 de massa seca e de magnésio de 4,2 g kg-1. / Two experiments were carried out in greenhouse conditions with Panicum maximum cv. Mombaça grown in nutrient solution, using ground quartz as substrate. One experiment had eight potassium rates (0; 9.75; 39; 78; 156; 234; 312 and 468 mg L-1) and another had eight magnesium rates (0; 1.2; 3.6; 12; 24; 36; 48 and 60 mg L-1) and were performed from January to March in each year. The objective was to evaluate the effects of potassium and magnesium rates on dry matter yield, tiller number, leaf area, chlorophyll concentration in the leaves throught SPAD readings, accumulation and concentration of each nutrient in plant components, and to estabilish parameters to determine the critical level of these two nutrients. In each experiment a complete randomized block design was used, with four replications. There was a positive effect of potassium rates in the dry matter yield of plant tops and roots, that fitted on quadratic models and in the leaf area expressed by linear models. Potassium rates affected the tiller number, but did not influence the estimated chlorophyll concentration in leaves. Potassium concentration in the emerging leaves, lamina of recently expanded leaves, lamina of mature leaves, stems plus sheaths and roots increased with potassium increasing in the nutrient solution. The highest and lowest potassium accumulation in the dry matter of plant tops were observed in stems plus sheaths and lamina of mature leaves, respectively. A positive effect of magnesium rates was observed in the dry matter of plant tops and root, in leaf area, in chlorophyll concentration, and they fitted in quadratic and linear models according to the studied variable. Magnesium rates in solution increased the tiller number up to 48 mg L-1. The concentration of magnesium in the emerging leaves, lamina of recently expanded leaves, lamina of mature leaves, stems plus sheaths and roots increased with magnesium solution from 0 to 60 mg L-1. The highest and lowest accumulation of magnesium in the dry matter of plant tops were observed in the stems plus sheaths and emerging leaves, respectively. The lamina of recently expanded leaves showed to be suitable for the evaluation of the nutricional status in potassium and magnesium of the Mombaça grass and the critical level were 15.4 and 17.3 g kg-1 for potassium and 4.2 g kg-1 for magnesium.

As pastagens são a principal fonte de alimento para o rebanho bovino no Brasil e elas são constituídas principalmente por gramíneas. Entre as espécies dominantes a Panicum maximum destaca-se pela sua boa produtividade e alto valor nutritivo. Para a formação e a manutenção dos pastos, o fornecimento e o balanço entre nutrientes na planta assumem importância fundamental. Neste estudo objetivou-se avaliar as respostas do Panicum maximum cv. Tanzânia, a combinações de doses de fósforo e de zinco na solução nutritiva, quanto aos parâmetros morfofisiológicos e produtivos e às concentrações de fósforo e zinco nos tecidos. Realizou-se um experimento na casa-de-vegetação localizada em Piracicaba, Estado de São Paulo, com o capim-Tanzânia no período de verão. Foram testadas cinco doses de fósforo (0,2, 0,6, 1,0, 1,4 e 1,8 mmol L-1) e cinco de zinco (0,00, 0,25, 0,50, 0,75, 1,00 µmol L-1) num estudo em superfície de resposta, baseado no desenho experimental composto central modificado de um esquema fatorial 52 incompleto resultando em 13 combinações de doses. Para os demais nutrientes as soluções foram baseadas na completa de Sarruge (1975) modificada apenas para nitrogênio fornecido na dose de 30 mmol L-1. As trocas de soluções foram efetuadas a cada 14 dias. Colocaram-se cinco plantas em vasos de 3,6 L, utilizando-se sílica como substrato. Aos 32 dias após o transplante procedeu-se o primeiro corte das plantas e aos 28 dias após o primeiro realizou-se o segundo corte. Imediatamente após cada corte o material vegetal foi separado em: folhas emergentes (FE), lâminas de folhas recém-expandidas (LR), lâminas de folhas maduras (LM) e colmos + bainhas (CB). Após o segundo corte, realizou-se a coleta das raízes. As partes das plantas coletadas nos dois cortes foram colocadas para secar, determinou-se a massa seca das plantas e a moagem para posterior análise no laboratório. Os resultados mostraram significâncias para a interação entre as doses de fósforo e as de zinco na massa seca da parte aérea do primeiro corte, na área foliar do segundo corte, na massa seca de raízes, nas concentrações de fósforo nas LM coletadas no primeiro e segundo cortes, na concentração média de fósforo na parte aérea das plantas e na concentração de zinco nas FE no primeiro crescimento. A massa seca da parte aérea no segundo crescimento, a área foliar no primeiro crescimento, os números de folhas e perfilhos no primeiro e segundo crescimentos, valor SPAD no primeiro crescimento, comprimento, comprimento específico e superfície de raízes, concentração de fósforo nas FE, LR e CB no primeiro e segundo crescimentos, concentração média de fósforo na parte aérea no segundo crescimento, concentração de fósforo nas raízes, concentração de zinco nas LR, LM e CB coletada tanto no primeiro como no segundo cortes e concentração de zinco nas FE amostradas no segundo corte variou significativamente com as doses de fósforo na solução. As doses de zinco influenciaram significativamente a área foliar e a concentração de zinco nas LM do primeiro corte. / Pastures are the main food source for de cattle in Brazil, and the grasses occupy most of them. Among the species in those pastures, Panicum maximum is preferred for its productivity and quality. In order to establish and maintain the pastures, it is necessary to supply nutrients in balanced amounts. The objective of this study was to determine the Panicum maximum cv. Tanzânia responses to combinations of phosphorus and zinc rates in nutrient solution related to the morphophysiology, yield and the concentrations of phosphorus and zinc in plant tissues. An experiment was carried out in a greenhouse located at Piracicaba, State of Sao Paulo, Brazil, with Tanzânia grass, in Summer season. Five rates of phosphorus (0.2, 0.6, 1.0, 1.4 e 1.8 mmol L-1) and five rates of zinc (0.00, 0.25, 0.50, 0.75, 1.00 µmol L-1) were studied in a response surface methodology, based on a modified central composite design of a fractional 52 factorial. Other nutrients had concentrations in solution as recommended by Sarruge (1975), except for nitrogen that was supplied at 30 mmol L-1. Five plants were grown in 3.6 L plastic pots, containing ground quartz. The first harvest was done at 32 days after seedlings transplanting to the pots, and the second harvest was accomplished at 28 days after the first one. At the time of each harvest plant tops were separated into: emergents leaves (EL), lamina of recently expanded leaves (RL), lamina of mature leaves (ML) and colms + sheaths (CS). After the second harvest the roots werw collected. All plant material was dried, weighed and ground for laboratory analysis. The results showed that the interaction between phosphorus and zinc rates was significant for the dry matter yield at the first harvest, leaf area at the second harvest, roots dry weight, phosphorus concentration in the ML at the first and second harvests, in both the average phosphorus concentration in plant tops and zinc concentration in EL at the first harvest. Dry matter yield at the second harvest, leaf area at the first harvest, numbers of tillers and leaves at the first and second harvest , SPAD value in the first harvest, roots length, specific length and surface, phosphorus concentrations in the EL, RL and CS at the first and second harvests, the average phosphorus concentration in plant tops at the second harvest, phosphorus concentration in the roots, zinc concentrations in the RL, ML, and CS at the first and second harvests, and zinc concentration in the EL at the second harvest significantly changed with phosphorus rates. Significant responses to zinc rates were obtained for leaf area and zinc concentration in ML in first growth.

Orientador: Iraê Amaral Guerrini / Coorientador: Agnaldo Scarassati / Banca: Clarice Backes / Banca: Edivaldo Casarini / Resumo: O presente estudo teve por objetivo comparar os sistema hidropônico e o de fertirrigação no desenvolvimento de mudas da espécie florestal nativa jequitibábranco (Cariniana estrellensis). O experimento foi conduzido no Viveiro da Fazenda Experimental Cantareira, pertencente à Faculdade Integral Cantareira (FIC), no município de Mairiporã - SP - setor de Pesquisa Florestal. Foi estudado o desenvolvimento da Cariniana estrellensis sob dois sistemas de produção: fertirrigação e hidropônico. Os sistemas tiveram quatro parcelas com 25 plantas cada, totalizando 100 plantas por sistema. Para avaliação morfológica não destrutiva realizada a cada 14 dias, considerou-se a altura da planta e o diâmetro do colo das 100 plantas de cada sistema, totalizando 200 plantas e, ao término do experimento, para avaliação morfológica destrutiva, foram consideradas como plantas úteis as 9 centrais, totalizando 36 plantas úteis de cada sistema. A qualidade das mudas produzidas no sistema hidropônico foi superior àquelas do sistema de fertirrigação para os seguintes parâmetros e relações: diâmetro do coleto (D), altura da parte aérea (H), massa seca da parte aérea (MSPA), massa seca total (MST), área foliar (AF), relação altura e diâmetro (H/D), altura e massa seca da parte aérea (H/MSPA), massa seca da parte aérea e do sistema radicular (MSPA/MSR), e o Índice de Qualidade Dickman (IQD). Em função dos resultados obtidos, pode-se inferir que para Cariniana estrellensis o sistema hidropônico proporcionou melhor desenvolvimento, reduzindo o período de produção das mudas / Abstract: This study aimed to compare hydroponics and fertigation systems on seedlings production of native species (jequitibá-branco, Cariniana estrellensis). The experiment was conducted at Cantareira Experimental Farm Nursery, owned by Integral Cantareira College (FIC) at the Forestry Research Sector of Mairiporã, SP. We studied the development of jequitibá branco under two production systems: drip irrigation and hydroponics. The systems presented four plots with 25 plants each, totaling 100 plants per system. It was considered for the non-destructive morphological evaluation the plant height and stem diameter of 100 plants per system, totaling 200 plants. This procedure was conducted every 14 days. At the end of the experiment, the morphological destructive assessment was performed on the central 9 plants, with a total of 36 useful plants per system. The quality of seedlings produced in the hydroponic system was superior to the fertigation system for the following parameters and relations: the collar diameter (D), shoot height (H), shoot dry mass (MSPA), total dry mass (MST), leaf area (LA), height and diameter ratio (H/D), dry mass of shoot and root system (MSPA/MSR), and Dickman Quality Index (IQD). Based on the results, we can infer that the hydroponic system enhanced Cariniana estrellensis, growth reducing the period of seedlings production / Mestre

Submitted by Erika Demachki (erikademachki@gmail.com) on 2014-09-30T20:15:38Z No. of bitstreams: 2 Tese - Graciella Corcioli - 2013.pdf: 3776229 bytes, checksum: 98c9f8e9f50170e8fc0e65039fb84432 (MD5) license_rdf: 23148 bytes, checksum: 9da0b6dfac957114c6a7714714b86306 (MD5) / Approved for entry into archive by Jaqueline Silva (jtas29@gmail.com) on 2014-09-30T21:43:30Z (GMT) No. of bitstreams: 2 Tese - Graciella Corcioli - 2013.pdf: 3776229 bytes, checksum: 98c9f8e9f50170e8fc0e65039fb84432 (MD5) license_rdf: 23148 bytes, checksum: 9da0b6dfac957114c6a7714714b86306 (MD5) / Made available in DSpace on 2014-09-30T21:43:30Z (GMT). No. of bitstreams: 2 Tese - Graciella Corcioli - 2013.pdf: 3776229 bytes, checksum: 98c9f8e9f50170e8fc0e65039fb84432 (MD5) license_rdf: 23148 bytes, checksum: 9da0b6dfac957114c6a7714714b86306 (MD5) Previous issue date: 2013-03-08 / The african mahogany (Khaya ivorensis) is a high value forest essence in the international trade and of great potential to replace the Brazilian mahogany (Swietenia macrophylla), which is one of the most valuable commercial species in the world, but presents serious difficulties in its cultivation. Currently, little is known about the nutritional requirements of the african mahogany. In this sense, this study aimed to characterize and induce the symptoms of the deficiencies of macro nutriens (nitrogen, phosphorus, potassium, calcium, magnesium, sulfur) and micro nutrients (iron, boron, copper, manganese, molybdenum and zinc) based on the diagnosis by the visual method and by means of chemical analysis on seedlings of african mahogany. In order to do that, two experiments were conducted with a nutritive solution with the following treatments: T1-Complete Solution; T2-Complete solution, with the omission of nitrogen; T3-complete solution, with the omission of phosphorus; T4-complete solution, with the omission of potassium, T5-complete solution, with the omission of calcium; T6-complete solution, with the omission of magnesium-T7 complete solution, with the omission of sulfur; T8-complete solution, with the omission of iron; T9-complete solution, with the omission of boron, T10-complete solution, with the omission of copper; T11-complete solution, with the omission of manganese; T12-complete solution, with the omission of zinc; T13-complete solution, with the omission of molybdenum and T14-Witness (deionized water). In Experiment 1 the evaluations were carried from the emergence of seedlings obtained by seed that were put to germinate in coarse washed sand and irrigated with deionized water until sixty days after sowing, starting then to receive daily 200 ml of nutrient solution. The experimental design used was completely randomized with fourteen treatments and ten replications. The dendrological data and the visual symptoms were described and photographed every fifteen days from the initial stage until they became well defined at 210 days. In Experiment 2 the evaluations were carried from commercial seedlings ready for the field planting, which were removed from the 180 cm3 tubes and transferred to the plastic tubes with capacity for 4.8 L, containing coarse washed sand as substrate. These seedlings remained in the greenhouse for fifteen days, just being irrigated with deionized water. After this period the process of rustification of the seedlings was begun to subsequently start up the daily irrigation with 200 ml of nutrient solution, described in the Experiment 1. The experimental design used was completely randomized with fourteen treatments and ten replications. The dendrological data and the visual symptoms were described and photographed every fifteen days from the initial stage until they became well defined, at 220 days. At forty days after the start of the irrigation with nutrient solutions,the first visual symptoms begin to revel, with easy visualization and characterization. In Experiment 1 isolated omissions of macronutrients and micronutrients respective induces deficiency symptoms associated with smaller contents (except Cu and Mg), but only the absence of N, P, Fe, Cu and B significantly affect plant development. In Experiment 2 the plants from seedlings ready for planting the development is affected only by the omission of N in the nutrient solution, showing deficiency symptoms and smaller contents of the element. The omissions of the other nutrients reaches their induce mild symptoms of nutritional deficiency typical and lower foliar Ca and Fe only. / O mogno africano (Khaya ivorensis) é uma essência florestal de alto valor no comércio internacional e de grande potencial para substituir o mogno brasileiro (Swietenia macrophylla), que é uma das espécies de maior valor comercial do mundo, mas apresenta sérias dificuldades em seu cultivo. Atualmente, pouco se conhece a respeito das exigências nutricionais do mogno africano. Nesse sentido, o presente trabalho teve o objetivo de induzir e caracterizar os sintomas de deficiências de macronutrientes (nitrogênio, fósforo, potássio, cálcio, magnésio e enxofre) e micronutrientes (ferro, boro, cobre manganês, zinco e molibdênio), através do diagnóstico pelo método visual e por meio de análise química em mudas de mogno africano. Para isso, realizaram-se dois experimentos com solução nutritiva, com os seguintes tratamentos: T1-Solução completa; T2-Solução completa, com omissão de nitrogênio; T3-Solução completa, com omissão de fósforo; T4-Solução completa, com omissão de potássio; T5-Solução completa, com omissão de cálcio; T6-Solução completa, com omissão de magnésio; T7-Solução completa, com omissão de enxofre; T8-Solução completa, com omissão de ferro; T9-Solução completa, com omissão de boro; T10-Solução completa, com omissão de cobre; T11-Solução completa, com omissão de manganês; T12-Solução completa, com omissão de zinco; T13-Solução completa, com omissão de molibdênio e T14-testemunha (água deionizada). No Experimento 1 as avaliações foram realizadas a partir da emergência das plântulas obtidas de sementes, que foram colocadas para germinar em areia grossa lavada e irrigadas com água deionizada até os sessenta dias após a semeadura, quando passaram a receber 200 ml de solução nutritiva diariamente. O delineamento experimental utilizado foi inteiramente casualizado com quatorze tratamentos e dez repetições. Os dados dendrológicos e os sintomas visuais foram descritos e fotografados a cada quinze dias desde o estágio inicial até tornarem-se bem definidos, aos 210 dias. No Experimento 2 as avaliações foram realizadas a partir de mudas comerciais prontas para o plantio no campo, retiradas dos tubetes de 180 cm3 e transferidas para tubetes com capacidade para 4,8 L, contendo como substrato areia grossa lavada. Essas mudas permaneceram em casa de vegetação por quinze dias, sendo irrigadas apenas com água deionizada. Após esse período iniciou-se o processo de rustificação das mudas para, posteriormente, iniciar-se a irrigação diária com 200 ml de solução nutritiva, descritas para o Experimento 1. O delineamento experimental utilizado foi inteiramente casualizado com quatorze tratamentos e dez repetições. Os dados dendrológicos e os sintomas visuais foram descritos e fotografados a cada quinze dias desde o estágio inicial até tornarem-se bem definidos, aos 220 dias. Aos quarenta dias após o início da irrigação com as soluções nutritivas, os primeiros sintomas visuais começam a se evidenciar, sendo de fácil visualização e caracterização. No Experimento 1 as omissões isoladas dos macronutrientes e micronutrientes induzem respectivos sintomas visuais de deficiência associados a menores teores foliares (exceto de Mg e Cu), mas somente as ausências de N, P, Fe, B e Cu afetam significativamente o desenvolvimento das plantas. No Experimento 2 as plantas oriundas de mudas prontas para o plantio tem o desenvolvimento afetado apenas pela omissão de N na solução nutritiva, exibindo sintomas visuais de deficiência e menores teores foliares do elemento. As omissões dos demais nutrientes chegam a induzir sintomas leves de deficiência nutricional característicos e menores teores foliares apenas de Ca e Fe.

As pastagens são a principal fonte de alimento para o rebanho bovino no Brasil e elas são constituídas principalmente por gramíneas. Entre as espécies dominantes a Panicum maximum destaca-se pela sua boa produtividade e alto valor nutritivo. Para a formação e a manutenção dos pastos, o fornecimento e o balanço entre nutrientes na planta assumem importância fundamental. Neste estudo objetivou-se avaliar as respostas do Panicum maximum cv. Tanzânia, a combinações de doses de fósforo e de zinco na solução nutritiva, quanto aos parâmetros morfofisiológicos e produtivos e às concentrações de fósforo e zinco nos tecidos. Realizou-se um experimento na casa-de-vegetação localizada em Piracicaba, Estado de São Paulo, com o capim-Tanzânia no período de verão. Foram testadas cinco doses de fósforo (0,2, 0,6, 1,0, 1,4 e 1,8 mmol L-1) e cinco de zinco (0,00, 0,25, 0,50, 0,75, 1,00 µmol L-1) num estudo em superfície de resposta, baseado no desenho experimental composto central modificado de um esquema fatorial 52 incompleto resultando em 13 combinações de doses. Para os demais nutrientes as soluções foram baseadas na completa de Sarruge (1975) modificada apenas para nitrogênio fornecido na dose de 30 mmol L-1. As trocas de soluções foram efetuadas a cada 14 dias. Colocaram-se cinco plantas em vasos de 3,6 L, utilizando-se sílica como substrato. Aos 32 dias após o transplante procedeu-se o primeiro corte das plantas e aos 28 dias após o primeiro realizou-se o segundo corte. Imediatamente após cada corte o material vegetal foi separado em: folhas emergentes (FE), lâminas de folhas recém-expandidas (LR), lâminas de folhas maduras (LM) e colmos + bainhas (CB). Após o segundo corte, realizou-se a coleta das raízes. As partes das plantas coletadas nos dois cortes foram colocadas para secar, determinou-se a massa seca das plantas e a moagem para posterior análise no laboratório. Os resultados mostraram significâncias para a interação entre as doses de fósforo e as de zinco na massa seca da parte aérea do primeiro corte, na área foliar do segundo corte, na massa seca de raízes, nas concentrações de fósforo nas LM coletadas no primeiro e segundo cortes, na concentração média de fósforo na parte aérea das plantas e na concentração de zinco nas FE no primeiro crescimento. A massa seca da parte aérea no segundo crescimento, a área foliar no primeiro crescimento, os números de folhas e perfilhos no primeiro e segundo crescimentos, valor SPAD no primeiro crescimento, comprimento, comprimento específico e superfície de raízes, concentração de fósforo nas FE, LR e CB no primeiro e segundo crescimentos, concentração média de fósforo na parte aérea no segundo crescimento, concentração de fósforo nas raízes, concentração de zinco nas LR, LM e CB coletada tanto no primeiro como no segundo cortes e concentração de zinco nas FE amostradas no segundo corte variou significativamente com as doses de fósforo na solução. As doses de zinco influenciaram significativamente a área foliar e a concentração de zinco nas LM do primeiro corte. / Pastures are the main food source for de cattle in Brazil, and the grasses occupy most of them. Among the species in those pastures, Panicum maximum is preferred for its productivity and quality. In order to establish and maintain the pastures, it is necessary to supply nutrients in balanced amounts. The objective of this study was to determine the Panicum maximum cv. Tanzânia responses to combinations of phosphorus and zinc rates in nutrient solution related to the morphophysiology, yield and the concentrations of phosphorus and zinc in plant tissues. An experiment was carried out in a greenhouse located at Piracicaba, State of Sao Paulo, Brazil, with Tanzânia grass, in Summer season. Five rates of phosphorus (0.2, 0.6, 1.0, 1.4 e 1.8 mmol L-1) and five rates of zinc (0.00, 0.25, 0.50, 0.75, 1.00 µmol L-1) were studied in a response surface methodology, based on a modified central composite design of a fractional 52 factorial. Other nutrients had concentrations in solution as recommended by Sarruge (1975), except for nitrogen that was supplied at 30 mmol L-1. Five plants were grown in 3.6 L plastic pots, containing ground quartz. The first harvest was done at 32 days after seedlings transplanting to the pots, and the second harvest was accomplished at 28 days after the first one. At the time of each harvest plant tops were separated into: emergents leaves (EL), lamina of recently expanded leaves (RL), lamina of mature leaves (ML) and colms + sheaths (CS). After the second harvest the roots werw collected. All plant material was dried, weighed and ground for laboratory analysis. The results showed that the interaction between phosphorus and zinc rates was significant for the dry matter yield at the first harvest, leaf area at the second harvest, roots dry weight, phosphorus concentration in the ML at the first and second harvests, in both the average phosphorus concentration in plant tops and zinc concentration in EL at the first harvest. Dry matter yield at the second harvest, leaf area at the first harvest, numbers of tillers and leaves at the first and second harvest , SPAD value in the first harvest, roots length, specific length and surface, phosphorus concentrations in the EL, RL and CS at the first and second harvests, the average phosphorus concentration in plant tops at the second harvest, phosphorus concentration in the roots, zinc concentrations in the RL, ML, and CS at the first and second harvests, and zinc concentration in the EL at the second harvest significantly changed with phosphorus rates. Significant responses to zinc rates were obtained for leaf area and zinc concentration in ML in first growth.

Submitted by Celso Magalhaes (celsomagalhaes@ufrrj.br) on 2018-09-20T17:04:32Z No. of bitstreams: 1 2016 - Nubia Valle Mezzavilla.pdf: 2915573 bytes, checksum: f7b585622179c2b55f0b4f83a2d41bda (MD5) / Made available in DSpace on 2018-09-20T17:04:32Z (GMT). No. of bitstreams: 1 2016 - Nubia Valle Mezzavilla.pdf: 2915573 bytes, checksum: f7b585622179c2b55f0b4f83a2d41bda (MD5) Previous issue date: 2016-06-29 / Conselho Nacional de Desenvolvimento Cient?fico e Tecnol?gico - CNPQ / Studies of aluminum tolerance in seedlings aroeira-vermelha (Schinus terebinthifolius R.) were held in a growth chamber in the Department of Plant Science ? Laboratory of Chemistry of Rhizosphere in the Agronomy Institute of University Federal Rural of Rio de Janeiro. Experiments were carried out with different concentrations of aluminum in simple nutrient solution (with calcium) and a complete nutrient solution with concentrations range from 0, 25, 50, 100, 200, 400 and 800 ?M in the solution and in soil Oxisol which was added liming, P, K, and micronutrients. The liming varied from (zero, 500, 1000.2000 and 4000 kg ha-1 and with100, 60, 30 kg ha-1 of phosphorus, potassium and micronutrient Br-12, respectively), and a control treatment without liming, P, K and micronutrients. The evaluations were based on analysis of root growth using the root length parameters, root growth rate, relative and evaluated root elongation also shoot length, dry weight of shoot and root, stem diameter, pH of the rhizosphere and soil . It was found that it is important to time days after sowing, before the transfer of seedlings to the nutrient solution; it is indicated 18 days after sowing for simple nutrient solution and 15 days after sowing for complete solution. Root growth rate and relative root elongation are methods that should be recommended in studies with plants aroeira-vermelha. Complete diluted and balanced nutrient solutions, should be recommended in aluminum toxicity studies in this species rather than simple solutions only with calcium due to low potential for seed storage. Low concentration of Al simple solution led to increase root growth. The root length of Schinus terebinthifolius R. seedlings grown nutrient solutions paralyzed in concentration 131.87 87 ?M of Al. In the simple nutrient solution was only possible to establish the critical level of toxicity using the root growth rate obtaining the value of 88, 42 87 ?M aluminum. Schinus terebinthifolius R. response the application of liming and P, K and micronutrients when grown in a soil Oxisol substrate, and the dosage of 2164.64 kg ha-1 of lime, dosing which was obtained the highest root length. For dry weight of shoot of seedlings, only 1831.50 Kg ha-1 of lime was enough to hit the highest value / Os estudos da toler?ncia ao alum?nio em pl?ntulas de aroeira-vermelha (Schinus Terebinthifolius R.) foram realizados, em c?mara de crescimento no Laborat?rio de Qu?mica da Rizosfera no Departamento de Fitotecnia, Instituto de Agronomia da Universidade Federal Rural Rio de Janeiro. Foram realizados experimentos com diferentes concentra??es de Al em solu??o nutritiva simples (com c?lcio) e completa com concentra??es que variaram de 0, 25, 50, 100, 200, 400 e 800 ?M na solu??o e em solo Latossolo vermelho amarelo onde foi adicionado calagem, P, K, e micronutrientes. As dosagens de calagem variaram de tratamentos com calagem (0, 500, 1000, 2000 e 4000 Kg ha-1, sendo adicionados 100, 60 e 30 Kg ha-1de fosforo, pot?ssio e de micronutriente BR-12 respetivamente), al?m de um tratamento controle, sem calagem, P, K e micronutrientes. As avalia??es foram baseadas na an?lise do crescimento radicular utilizando os par?metros comprimento radicular, taxa de crescimento radicular, elonga??o radicular relativa e avaliados tamb?m o comprimento da parte a?rea, massa seca da parte a?rea e radicular, di?metro do colo, pH da rizosfera e do solo. Foi verificado que ? importante o tempo de dias ap?s a semeadura, antes da transfer?ncia das pl?ntulas para a solu??o nutritiva, sendo indicados 18 dias ap?s a semeadura para solu??o nutritiva simples e 15 dias ap?s a semeadura para solu??o completa. A taxa de crescimento radicular e a elonga??o radicular relativa s?o m?todos que devem ser recomendados em estudos com plantas de aroeira-vermelha. Solu??es nutritivas completas, dilu?das e balanceadas, devem ser recomendadas em estudos de toxidez de alum?nio nesta esp?cie ao inv?s de solu??es simples apenas com c?lcio, devido ao baixo potencial de reserva da semente. Baixas concentra??es de Al na solu??o simples, estimulou o crescimento de raiz. O comprimento de raiz de pl?ntulas de aroeira crescidas em solu??es nutritivas completas paralisou seu crescimento na concentra??o de 131,87 ?M de Al. Na solu??o simples s? foi poss?vel estabelecer o n?vel cr?tico de toxidez utilizando a taxa de crescimento radicular, obtendo o valor de 88,42 ?M de alum?nio. A aroeira respondeu a aplica??o de calagem, P, K e micronutrientes quando crescida em solo Latossolo Vermelho Amarelo, sendo a dosagem de 2164,64 Kg ha-1 de calc?rio a que obteve o maior comprimento radicular. Para a massa seca da parte a?rea somente 1831,50 Kg ha-1 de calc?rio foi suficiente para ser atingido o maior valor.

Traditionally, the condition of hydroponic nutrient solutions used in controlled environment or greenhouse plant production is obtained by monitoring solution pH and electrical conductivity. These parameters provide greenhouse operators with an overall sense of the solution quality, but do not provide information on individual components of the fertilizer solution. Considering that plant nutrient uptake requirements vary with crop, growth phase and environment, real-time ion-selective measurements represent a significant technological advance in the management of recirculating nutrient solutions. The capacity for the monitoring and control of individual ions in the nutrient solution is the last remaining environment variable for which reliable feedback control does not exist in controlled environment plant production. This technology development study focussed on optical ion-selective sensors, termed ‘optrodes’, for potassium and calcium sensing within nutrient solution. Bulk optrode absorption-based sensors, tailored explicitly for nutrient ion activities within typical hydroponic solutions were theoretically modelled, fabricated and experimentally tested. Experimental data on sensor response time, measuring range, selectivity, repeatability, lifetime and influence of temperature were compiled. These ion-selective bulk optrode membranes were integrated into a custom instrument combining a light source, mini-spectrometer, optical fibres and other associated components. Monitoring and control software was written that collected and converted optical measurements into estimated nutrient ion activities for use in managing nutrient solution quality. Implemented spectral averaging, normalization and ratiometric computations and techniques resulted in sensors that offered the advantage of auto-calibration. The sensors met selectivity requirements in half strength Hoagland nutrient solution and provided repeatable measurements in solutions of different ion activity levels. Potassium and calcium selective membranes demonstrated lifetimes of 30 to 50 hours when constantly immersed in nutrient solution. Strategies for their operational implementation in plant growth systems based upon experimental testing are proposed as are suggestions for future study. The low mass, low volume, low cost and robust nature of these optrodes offer a promising technology for future space-based plant production systems and give them excellent terrestrial technology transfer potential.