O presente estudo objetiva avaliar os mecanismos de transferência de carbono entre os compartimentos atmosfera, vegetação, solo e igarapé em uma microbacia da Amazônia Ocidental. Dois igarapés drenandos, respectivamente, 2927 e 66,73 ha de floresta de terra firme, foram monitorados durante um ano. A área de estudo se encontra na zona de amortecimento de uma Unidade de Conservação de Uso Sustentável denominada Floresta Estadual do Amapá (FLOTA/AP), na região central do estado de mesmo nome. Foram coletadas as águas da chuva, da precipitação interna da floresta, do escoamento de água pelo tronco, do escoamento superficial pelo solo, da solução do solo, da água subterrânea e da água do igarapé. Os solos também foram investigados em relação a suas características físico-químicas. Para calcular a entrada e saída de C do sistema, foram determinadas as concentrações do carbono orgânico e inorgânico dissolvido (COD e CID, respectivamente) na água da chuva e do igarapé, em 16 eventos de chuva. As concentrações médias de COD na água da chuva foram de 1,6± 1,52 mg L-1, resultando em um aporte de 11,43 Kg C ha-1 ano-1. Na precipitação interna os valores médios observados foram de 9,1 ± 5,99 mg L-1, o que corresponde a um fluxo de 100,71 Kg C ha-1 ano-1. No escoamento do tronco, os valores médios observados foram de 17,4 ± 8,03 mg L-1 e no escoamento superficial do solo de 14,2 ± 6,4 mg L-1. Nos compartimentos amostrados abaixo do solo, solução do solo e água do lençol, as concentrações de COD foram relativamente mais baixas. A saída de COD pelo igarapé, os fluxos foram de 0,45 Kg C ha-1 ano-1. Em relação às concentrações de CID, o aporte pela água da chuva foi de 3,66 Kg C ha-1 ano-1, passando a 10,10 Kg C ha-1 ano-1 na precipitação interna e com uma saída pelo igarapé de 0,07 Kg C ha-1 ano-1. Os resultados mostram grande variabilidade espaço-temporal e retenção de C pelo sistema, seja na fase orgânica (COD) ou inorgânica (CID), demonstrando a importância destes processos para a compreensão do funcionamento destes ecossistemas. / This study aims to evaluate carbon transfer mechanisms between the atmosphere, vegetation, soil and stream in a microbasin of eastern Amazon. Two streams, draining respectively 2917 and 66.73 ha of \"terra firme\" forests were monitored during one year. The study area is located in a Conservation Unit named Amapá State Forest (FLOTA/AP), in the central region of the Amapá State. We sample rain water, throughfall, stemflow, soil surface flow, soil solution, groundwater and stream water. Physico-chemical characteristics of soils were also evaluated. To calculate inputs and outputs of C in this system, we determined the concentrations of dissolved organic and inorganic carbon (DOC and DIC, respectively) in rain and stream water during 16 rain events. Average concentrations of DOC in rain water were 1.6± 1.52 mg L-1, resulting in an input of 11.43 Kg C ha-1 year-1. Throughfall had average concentrations of 9.1 ± 5.99 mg L-1, which increased inputs to 100.71 Kg C ha-1 year-1. Stemflow had average concentrations of 17.4 ± 8.03 mg L-1 while those of soil surface flow were 14.2 ± 6.4 mg L-1. Bellow ground DOC concentrations were relatively lower. The export of DOC in stream water was 0.45 Kg C ha-1 year-1. In relation to DIC, the input from rain water was 3.66 Kg C ha-1 year-1, increasing to 10.10 Kg C ha-1 year-1 in throughfall and exiting the micro basin through the stream with a flux of 0.07 Kg C ha-1 year-1. The results show large spatiotemporal variations and C retention within the system, either in the organic (DOC) or inorganic (DIC) phases, showing the importance of these processes for the comprehension of the functioning of these ecosystems.

O aprimoramento das práticas agronômicas e a utilização de alta tecnologia nas lavouras contribuiu para os aumentos significativos na produtividade das culturas e, pode ter alterado a dinâmica de absorção e partição dos nutrientes pelas plantas. As recomendações oficiais de adubação para a cultura de milho no Brasil estão baseadas no teor crítico do nutriente no solo e são provenientes de literaturas mais antigas. Por serem dinâmicos, os fatores que interagem em um sistema de produção agrícola podem ser melhor estudados através de modelos matemáticos. A técnica de modelagem em agricultura pode expandir a compreensão das interações que ocorrem no sistema solo-planta-atmosfera. Sendo assim, o objetivo deste estudo é propor um modelo para o cálculo de recomendação de adubação da cultura de milho, a partir da modelagem da marcha de absorção de nutrientes, da evapotranspiração potencial da cultura e, da partição dos nutrientes nas plantas, levando em consideração a taxa de absorção de cada nutriente para cada genótipo em um determinado sistema de produção. O modelo proposto considera que a recomendação de adubação deve ser realizada com base na variação temporal da taxa de absorção de nutrientes, a qual define melhor a demanda da cultura, em comparação com o teor crítico dos nutrientes no solo. Para a validação do modelo, sugere-se um ensaio de campo comparando as metodologias. / The improvement of agronomic practices and the use of high technology in field crops contributed for their significant increases in productivity, and could also have altered the dynamics of nutrient uptake and partition by the plant. Official recommendations for fertilizer applications to the maize crop in Brazil are based on the critical soil nutrient content and are relatively outdated. Since the factors that interact in an agricultural production system are dynamic, mathematical modeling turns out to be an appropriate tool for these studies. Agricultural modeling can expand our knowledge about the interactions prevailing in the soilplant- atmosphere system. In this way, the objective of this study is to propose a model for fertilizer recommendation of the maize crop, based on modelling nutrient uptake, potential evapotranspiration of the crop, and the partition of nutrients in the plant, taking into account the nutrient uptake rate of each genotype in a given production system. The proposed model considers that the fertilizer recommendation should be made based on the temporal variation of the uptake rate, which should better define crop needs in relation to the classic recommendation based on the critical soil nutrient content. To validate the model an experiment is suggested to compare methodologies.

This study examined nonpoint source pollution via tributaries to Pineview Reservoir. Since few literature values of export coefficients are available for snowfall-dominated watersheds such as Pineview, locally scaled rates were quantified using an upstream-downstream bracketing technique. Nitrogen and phosphorus grab samples were manually collected and discharge measurements were conducted during the annual study period. Additionally, high-frequency monitoring sensors that measured EC, temperature, turbidity, and water level were deployed at the up- and downstream sites to represent short-duration transport events and to examine watershed processes on a more representative time scale. Daily nutrient loads were estimated from grab samples and flow rates using the Rank- Data (RD) distribution method and, using surrogate relationships for discharge and total phosphorus (TP) concentration from high-frequency sensors, half-hour TP loads were calculated. Short-duration snow melt events were identified by turbidity spikes and increased air temperatures as well as, in some cases, hydrograph peaks. During these events, export coefficients from 0.31 to 0.54 g TP/ha/hr were observed for low and high elevation snow melt events. While losses to ground water were observed for one study reach, the study reach with positive load gains had annual export coefficients of 0.018 g TP/ha/hr from high-frequency loads and 7.5e-5 g NOx-N/ha/hr, 3.5e-6 g SRP/ha/hr, and 9.1e-6 g TP/ha/hr from RD loads. These rates were 1,000 to 2,000 times greater than available literature values typical of rainfall-dominated watersheds. This study showed the importance of erosive, snow melt events on nutrient transport and the need for high-frequency monitoring representing short-duration events for accurate estimation of export coefficients. Management strategies to reduce nutrients from tributaries should focus on erosion control in the Pineview Reservoir watershed.

Data from 7285 individual participants in the USDA Spring Nationwide Food Consumption Survey were analyzed using the nutrient density concept and multiple regression procedures to evaluate the impact of socioeconomic status on the nutritional quality of foods consumed. For each socioeconomic group the average daily amount of nutrients consumed per 1000 kcal of food consumed were computed and compared with the Recommended Dietary Allowances which had been converted to single-value nutrient allowances per 1000 kcal. This nutrient density approach identified qualitative patterns of food consumption for selected income, region, urbanization, household size, race, employment and education groups as well as indicating the degree to which these groups met the RDA. Results demonstrated that socioeconomic status had relatively little impact upon the average nutrient density of diets consumed by the population. Income level had no statistically significant effect upon the nutritional quality of diets for any of the fourteen nutrients studied. Household size affected nutrient density consumption of fat, carbohydrate, vitamin s6 and vitamin C. Race affected calcium, magnesium, vitamin A and thiamin density of diets. Other socioeconomic factors were significantly related to only one or two nutrients. The differences in average nutrient density of diets resulted from differences in the quality of foods consumed from the Basic Four (nutrient-dense) Plus One (calorie-dense) food groups and not from differences in the percent contribution of these two food groups to nutrient intake per 1000 kcal. Average diets for all socioeconomic groups were below nutrient density standards for calcium, iron, magnesium, vitamin B6 and carbohydrate. There was also a high frequency of individuals with vitamins A and vitamin C intakes below nutrient density standards despite adequate group mean intakes per 1000 kcal for these nutrients. Results supported the hypothesis that, regardless of socioeconomic status, Americans consume diets that average very similar nutrient content per energy unit. This type of information contributes to a better understanding of dietary habits of Americans and provides a meaningful framework from which to establish guidelines for government agencies, nutrition educators and the food industry.

Ecosystems are highly variable in space and time. Understanding how spatial and temporal scales influence the patterns and processes occurring across watersheds presents a fundamental challenge to aquatic ecologists. The goal of this research was to elucidate the importance of spatial scale on stream structure and function within the Oksrukuyik Creek, an Arctic watershed located on the North Slope of Alaska (68°36’N, 149°12’W). The studies that comprise this dissertation address issues of scale that affect our ability to assess ecosystem function, such as: methodologies used to scale ecosystem measurements, multiple interacting scales, translation between scales, and scale-dependencies. The first methodological study examined approaches used to evaluate chlorophyll a in ethanol extracts of aquatic biofilms. Quantification of chlorophyll a is essential to the study of aquatic ecosystems, yet differences in methodology may introduce significant errors to its determination that can lead to issues of comparability between studies. A refined analytical procedure for the determination of chlorophyll a was developed under common acidification concentrations at multiple common reaction times. The refined procedure was used to develop a series of predictive equations that could be used to correct and normalize previously evaluated chlorophyll a data. The predictive equations were validated using benthic periphyton samples from northern Alaska and northwestern Vermont, U.S.A. The second study examined interaction and translation between scales by examining how normalization approaches affect measurements of metabolism and nutrient uptake in stream sediment biofilms. The effect of particle size and heterogeneity on rates of biofilm metabolism and nutrient uptake was evaluated in colonized and native sediments normalized using two different scaling approaches. Functional rates were normalized by projected surface area and sediment surface area scaling approaches, which account for the surface area in plan view (looking top-down) and the total surface area of all sediment particles, respectively. Findings from this study indicated that rates of biogeochemical function in heterogeneous habitats were directly related to the total sediment surface area available for biofilm colonization. The significant interactions between sediment surface area and rates of respiration and nutrient uptake suggest that information about the size and distribution of sediment particles could substantially improve our ability to predict and scale measurements of important biogeochemical functions in streams. The final study examined how stream nutrient dynamics are influenced by the presence or absence of lakes across a variety of discharge conditions and how catchment characteristics can be used to predict stream nutrients. Concentrations of dissolved organic carbon (DOC) and other inorganic nutrients were significantly greater in streams without lakes than in streams in with lakes and DOC, total dissolved nitrogen (TDN), and soluble reactive phosphorus concentrations increased as a function of discharge. Catchment characteristic models explained between 20% and 76% of the variance of the nutrients measured. Organic nutrient models were driven by antecedent precipitation and watershed vegetation cover type while inorganic nutrients were driven by antecedent precipitation, landscape characteristics and reach vegetation cover types. The developed models contribute to existing and future understanding of the changing Arctic and lend new confidence to the prediction of nutrient dynamics in streams where lakes are present.

To assess how urbanization impacts stream nutrient uptake, a series of instantaneous (i.e. slug) nutrient additions were conducted in 3 urban and 3 non-urban streams during open and closed canopy conditions. Single additions of N, P, and combined additions of N and P were performed at each site. These data were used to test the hypothesis that high N:P concentrations in urban streams would result in P-limited conditions, and to assess differences in nutrient uptake kinetics (i.e., the relationship between uptake and concentration) between urban and non-urban streams. The results show that there were no consistent differences in N vs. P limitation among urban and non-urban streams suggesting that ambient N:P ratios are not useful predictors of nutrient limitation at the ecosystem scale. Areal uptake rates of N in urban streams were greater than non-urban streams coinciding with elevated N concentrations. Conversely, areal uptake rates of P were similar between urban and non-urban streams because these systems have similar ambient concentrations of P. Urban and non-urban streams demonstrated similar uptake velocity and areal uptake rate responses to increasing nutrient concentrations. However, unique to this study, urban streams had greater uptake velocities at ambient nutrient concentrations. These findings suggest that urban streams could have a greater capacity for nutrient uptake over a broad range of nutrient concentrations, but prior work indicates that this capacity may be constrained by the duration of the nutrient addition.

Thesis (M.Sc. Agric. (Horticulture)) --University of Limpopo, 2008 / The mineral nutrition of indigenous crop species is not well documented like other known crop species, thus making it difficult for one to know how to plant and maintain the crops. Mmupudu (Mimusops zeyheri), which, happens to be a wild crop, is one of the indigenous trees of interest to the Discipline of Plant Production, University of Limpopo. The current study gives baseline information that will be important in various environmental physiology studies of this plant. Physiological studies will be necessary to assess the importance of “limiting” mineral nutrients in the accumulation of certain mineral nutrients in Mmupudu in relation to its productivity. The experiment was arranged as a 2 x 3 factorial in RCBD, with the first and second factors being time of sampling and location, respectively. The three locations where data were collected were Chuenespoort, Bochum and Sekgosese. In each location, the experiment was replicated 10 times. Data were analyzed using ANOVA and means were separated using the least significant difference test. The two-factor interaction was nonsignificant (P ≥ 0.10) for both pH and electrical conductivity. Soil pH was not affected by time in all three locations suggesting that abscised flowers and fruitlets have no effect on pH. Leaf K experienced an increase of 65% at Chuenespoort and a decrease of soil K after fruiting by 44%. Leaf and soil P decreased after fruiting in all locations as was the case with Cu. Chuenespoort and Sekgosese experienced a decrease in leaf Mn after fruiting while soil Mn decreased in all whereas leaf Mg decreased in all locations. / the National Research Foundation and the Department of Water Affairs and Forestry

Due to recent changes in agricultural practices the nutrient load in our waterways has increased causing eutrophication and hypoxia. Many legislative actions have taken place because of this problem, including the Clean Water Act of 1972 (CWA), and many different nutrient reduction plans. The CWA governs that impaired waterways must be monitored to meet total maximum daily loads (TMDL) for each watershed. TMDL's must be assessed using data collected over a period of time so that reduction techniques can be administered. TMDL assessments are usually conducted by the United States Geological Survey (USGS) through many different monitoring programs. The USGS programs include collecting streamflow and nutrient concentration data and using the information to estimate nutrient loads. Generally, grab sampling is the method of choice for concentrations. Grab samples do not accurately assess the total load as generally only 6-8 samples can be collected over a year due to financial and logistical constraints, while concentrations vary within a span of hours and days. Research applications involve the use of automated sensors (e.g., ISCO) that allow for more frequent sampling in order to overcome this issue but are expensive to purchase and maintain. Thus the development of an inexpensive, passive sampler would be of much interest in estimating load. The Passive Surface Flux Meter (PSFM), an integrative sampler that estimates the total solute load over a storm event, is such an alternative. The PSFM is composed of two sorbents one to collect the contaminant of choice and another to determine the flow through the device. Ion-exchange resin was used to collect nitrates, while Granular Activated Carbon dosed with a suite of alcohols were used to determine flow through the sampler. This thesis sets forth the fundamental theories behind the PSFM, and investigates its ability to measure nutrient fluxes in the field. In-situ deployments within Clear Creek watershed in Iowa were conducted using a modification of the PSFM design by Boland (2011). There was a strong linear relationship between the loads estimated by the PSFM, and "true" load based on USGS stream gage data, and Nitratax sensor data. This implies that the device could be calibrated to work in the field. However, it was determined that the design underestimated the true load in the stream by 29%. This was attributed to the nonlinear relationship between the external velocity and the flow through the sampler, which weighted the results towards the high flow events. To overcome this constraint, a new design is proposed in which flow through the sampler varies linearly with the transient head at the inlet. Flume experiments done under different flow depths proved that linearity conditions were satisfied. Using the results from the laboratory experiments recommendations were made for design of an in-situ deployment of the new design.

In order to meet increasing water demands and more stringent regulations drinking water treatment plant managers must continually look to new treatment strategies and optimization techniques. One such strategy is to eliminate chlorine residual before filtration, allowing indigenous bacteria already present in the source water to grow on the filter media. These microorganisms help improve effluent water quality by increasing organic and inorganic contaminant removal. The process is known as biological filtration, or biofiltration. The implications of converting a conventional filtration plant (not specifically designed for biofiltration) to a biofiltration plant are still not well understood. Therefore, the purpose of this study was to evaluate water quality and operational trends of a side-by-side full-scale biofiltration conversion at the Quail Creek Water Treatment Plant (QCWTP), located in Hurricane, Utah, and to determine the impact of pre-chlorination elimination on filter performance. Four of twelve filters at the QCWTP were used to test the plant’s ability to operate in biological mode. One acted as a control and ran similar to the other eight filters in the treatment plant. The other three were converted to biofilters by quenching the influent chlorine residual with thiosulfate. The experiment lasted one year, so filter performance could be evaluated in each season. The results from the study indicated that the influent water was low in organic carbon (i.e. food for microorganisms), which resulted in small differences in biological activity between filters. Disinfection by-products (DBPs) (i.e. cancer causing agents created from the combination of chlorine and organic matter) were lower in the biofilters relative to the control. Biological conversion resulted in slightly higher and more variable final effluent turbidity values (though still within EPA drinking water standards and operational goals) compared to the non-biological filters; however, filter run times were unaffected.

Factors which affect iron bioavailability have been repeatedly and extensively investigated. A model, derived from these studies, has been developed for estimating available iron from meal data. However, many dietary surveys report only average daily intakes of iron, and do not report the iron present in single meals. No model to estimate available iron from daily iron intake has been presented in the literature. Dietary questionnaires were kept for two nonconsecutive weekdays by 355 male and 382 female Utah school children, mean age 7.5 years, assisted by their parents, and recorded by household measure. Data, first recorded as meals eaten, were used to develop three models for the estimation of available iron from total daily iron intake. It was concluded that available iron can be estimated from total iron intake by two of these models, as compared with the currently used model, which estimates available iron from data recorded by meal. Additionally, meal patterns of those factors involved with the estimation of available iron were investigated. The intake of dietary ascorbic acid and total iron was found to be evenly distributed among meals; approximately 10% of these nutrients was consumed as snacks. Of the meat, fish, poultry and the iron in those products consumed; 36% was taken at lunch, and 54% at dinner. Only 5% of the meat, fish, and poultry iron was consumed as snacks. The available iron distribution for breakfast, lunch, dinner, and snacks was 21.0%, 30.8%, 42.5% and 5.7%, respectively. Previous studies have investigated the characteristics of diets which provide 9 mg of iron per 1000 kcal of energy consumed. These diets have been shown to include larger portions of vegetables, fruits, and cereal products. In this study, these high-iron dense characteristics were studied as they pertain to total available iron intake. It was concluded that the high-iron dense diet receives more total available iron from the nonheme iron than from the heme iron consumed. Thus, it is conceivable that those dietary characteristics shown to provide a high-iron dense diet may also provide a high available iron intake.