Determining the relative roles of top-down vs. bottom-up forces in controlling the structure of ecological communities is of primary importance because anthropogenic nutrient loading, overharvesting of consumers, and potential interactions of these forces are pervasively changing ecosystems throughout the world. Here I use both field experimentation and meta-analyses to investigate the role of predators in controlling community composition, the relative roles of herbivores vs. nutrient enrichment in controlling the abundance of benthic primary producers, and the influence of herbivore diversity on the community structure of coral reefs. On a coral reef in the Florida Keys, I showed that release from predation by large fishes and invertebrates via exclusion cages allowed population increases in the gorgonian-eating gastropod Cyphoma gibbosum which increased predation rates on gorgonian corals. To directly address the relative roles of top-down and bottom-up forces in controlling primary producers in benthic marine habitats, I used factorial meta-analysis of 54 field experiments that orthogonally manipulated herbivore pressure and nutrient loading to quantify the effects of consumers and nutrient enrichment on community structure. The relative effects of herbivores vs. nutrient enrichment were context dependant, varying with latitude, the type of primary producer, and the nutrient status of the system. To address the influence of herbivore diversity on the community structure of Caribbean coral reefs, I used manipulative field experiments over two years to show that a Caribbean reef changes dramatically as a function of herbivorous fish diversity. The effects of herbivore diversity on community structure were strong in both years of the experiment due to different diet preferences among herbivores. Higher herbivore diversity suppressed macroalgal abundance, increased abundance of crustose coralline algae, reduced coral mortality, and increased coral growth when compared to treatments with lower herbivore diversity. Complementary feeding by different fishes drove these patterns because macroalgae were unable to effectively deter feeding by fishes with different attack strategies. Thus, herbivore diversity appears to play an important role in the healthy function of coral reef ecosystems via complementary feeding of different herbivore species.

The biological treatment is one of the commonly methods of wastewater treatment plant in wastewater treatment processes. The biological treatment can meet water quality standards required by the plant in response to different sewage conditions and qualities. It can purify high pollution loading sewage through the use of microbial metabolic transformation. Through effectively protecting and using water resources, the ecological balance of ocean and river can be maintained and environmental quality can be improved in consequence. This study analyzes the operations of a wastewater treatment plant, which is part of an urban sewage system. The major sources of inflow to the plant are domestic sewage, followed by rainfall runoff and industrial wastewater. The biological treatment system adopted in the plant is "Biological Nutrient Removal (BNR)". The reason for using BNR is to prevent eutrophication of downstream water bodies due to untreated nitrogen, phosphorus and other nutrient substances in discharged sewages. The design of BNR, which is called "A2O activated sludge method", would increase the anaerobic-anaerobic mixing process for simultaneous removal of the sewage of organic carbon, nitrogen, phosphorus and BOD. The study collected the data to analyze the impacts of extreme weather event, i.e. Typhoon Morakot, and the effects of newly developed industrial, i.e. polysilicon industry. Water quality data of inflow and outflow sewages starting from January 2009 to December 2011 were compiled to perform statistical analyses. By plotting various time series figures, the study can effectively explore the variations of pollutant removal under the two designated situations in the biological treatment system. The results show the abnormal increase in conductivity of effluent which has decreased pollutant removal since August 2010. Besides, the confluence of rainwater and sewage has severely affected the efficiency and quality of the biological treatment process during a typhoon or heavy rain event. This study has identified the potential impacts on a BNR plant which can provide the administration to enhance the effectiveness of the biological treatment plant and the function of sewage purification stability control.

A total of 40 beef arm chucks were collected from three cities across the United States to study the proximate composition of their separable lean. Chucks were fabricated 5-7 d postmortem and later cooked and dissected, or dissected raw into four separable components, separable lean, external fat, separable seam (intermuscular) fat, and connective tissue (considered inedible). Proximate analysis was conducted on the separable lean component of each dissected retail cut. Dissection data showed that multiple muscled cuts had a numerically lower percent separable lean when compared to the retail cuts comprised of a single muscle. Proximate analysis showed that as the mean value for moisture decreased in the retail cut, the mean percentage of total fat increased. Least squares means of total fat percentage were reported on the retail cuts stratified by USDA quality grade (upper Choice, lower Choice, and Select). Some of the retail cuts had significantly different total fat percentage of the separable lean when considering the differences in USDA quality grade. Cooking yields for the three methods utilized were numerically different. The cuts that were roasted had the highest cooking yield (80.72 %), followed by cuts that were grilled (76.58%), and finally cuts that were braised (66.13%). Differences in final endpoint temperature for each cut may account for the differences between cooking methods. This study was designed to acquire data to update the National Nutrient Database for Standard Reference, as well as to provide nutritional information for cuts that are not presently in the database. This study evaluated thirteen cooked cuts and twelve raw cuts in an effort to increase the number of retail cuts available to search for nutrient information in the National Database.

Grass-dominated ecosystems in many regions around the world have experienced increased abundance of woody plants during the past 100 yrs. In the Rio Grande Plains of southern Texas, subtropical woodlands, dominated by C3 trees/shrubs capable of symbiotic N2-fixation, have become significant components of landscapes that were once dominated by C4 grasslands. Upland areas in this region now consist of small discrete clusters and large groves of woody vegetation embedded in a grassy matrix, while lower-lying portions of the landscape are dominated by closed-canopy woodlands. I used soil δ13C in conjunction with aerial photography and geostatistics to quantify landscape-scale vegetation dynamics in uplands of this savanna parkland. Spatial patterns of soil δ13C in grids and transects traversing woody patches indicated larger woody groves were formed from small discrete clusters of woody plants that spread laterally and eventually coalesced. Soil δ13C contour maps revealed some clusters are currently growing rapidly towards each other and might coalesce into groves in the near future, while some clusters remained relatively stable. Kriged maps of soil δ13C provided a strong spatial context for future studies aimed at understanding the functional consequences of this change in landscape structure. The dominant invading woody plant, honey mesquite (Prosopis glandulosa), was important in determining the spatial pattern of soil δ13C, supporting the hypothesis that they serve as recruitment foci and facilitate the establishment of subordinate woody species. Leaf δ15N values suggested that the N2-fixing mesquite influenced the N nutrition of nearby non-N2-fixing shrubs, thus, suggesting a mechanism by which mesquite could facilitate establishment of other woody species. In closed-canopy drainage woodlands, however, spatial patterns of soil δ13C were no longer controlled by the presence of mesquite, but by the amount of soil organic carbon and soil texture. The positive correlation between silt+clay and soil δ13C indicates that the formation of organomineral complexes and microaggregates may slow SOC turnover rates and favor the persistence of C4-derived SOC from the original grassland. This study enhances our understanding of potential patterns, causes and consequences of grassland to woodland conversions which are underway today in many grass-dominated ecosystems around the world.

The purpose of this study was to collect and analyze retail cuts from the beef rib and plate that had been identified as needing nutrient composition updates in the United States Department of Agriculture’s (USDA) National Nutrient Database for Standard Reference (SR). Twenty beef carcasses were selected from three different regions of the United States, and the rib and plate were collected for shipment via refrigerated truck to the Rosenthal Meat Science and Technology Center. Each rib and plate was fabricated 14 to 21 d postmortem into the appropriate retail cuts to be used for this study. The cuts were dissected, either raw or cooked (braised, grilled, roasted), into four separable components: separable lean, seam fat, external fat, and refuse. Bone and heavy connective tissue were considered refuse. Percent total chemical fat, moisture, protein, and ash analyses were conducted on the separable lean component obtained from dissection. Cooking yields were evaluated for each of the three cooking methods utilized in this study. Grilled cuts had the highest numerical yield followed by roasted and braised cuts. Dissection data showed single muscle cuts had a higher percentage of separable lean than retail cuts composed of multiple muscles. Boneless and lip-off retail cuts contained a higher percentage of separable lean when compared to their bone-in and lip-on counterparts. Finally, proximate analysis data showed that as retail cuts increased in the percentage of total chemical fat, the percentage of moisture decreased. When percentage of total chemical fat was stratified by USDA quality grade, most cuts showed differences between USDA Choice and Select quality grades. This study was a collaborative project; therefore, the results and discussion of this thesis are only based on findings from Texas A and M University's data. The final project results will be published in the USDA’s National Nutrient Database SR.

Upflow anaerobic sludge blanket (UASB) has been developed and commercially used for treating high-strength wastewaters since 1971, and this process has been extended its applications to domestic sewage treatment since 1980. Constructed wetlands (CWL) can be treated as a simulated natural treatment system, which use solar radiation as the energy source. By analogy with some removal mechanisms in natural wetlands, constructed wetlands are able to transform and remove pollutants from the wastewater. Other features provided by the constructed wetland include wildlife support, hydrologic modification, erosion protection, and open space and aesthetics. It has been applied for domestic wastewater purification for decades. In this study, a pilot UASB-CWL reactor was built to test its performance for the removal of organic compounds and nutrients from a sewage with COD (Chemical oxygen demand) in the range of 200-300 mg/L. The UASB reactor has an active liquid volume of 2.5 liters and the CWL includes a 56 L-tank (CWL-1) followed by a 80 L-tank (CWL-2) in which emergent macrophyte (reed, Phragmites australis L.) and floating macrophyte (Pistia stratiotes L.), respectively, were planted. Effects of hydraulic retention time (HRT) of the test water in the reactor on the pollutant removal were tested. Results indicate that with HRTs of 2, 45, and 64 hours, respectively, in the UASB, CWL-1, and CWL-2, the system exhibited good performances for pollutant removal. The HRTs are equivalent to CODt loadings of 3.06, 0.065, and 0.026 kg/m3.day to the UASB, CWL-1, and CWL-2, respectively. Average total COD (CODt), soluble COD (CODs), total biochemical oxygen demand (BODt), suspended solids (SS), and phosphate-P (P) were 121, 62, 33, 130, and 0.10 mg/L, respectively, and these are equivalent to the removal efficacies of 45-65, 60-80,65-75, 30-50, and 85-95%, respectively. With the operation conditions, average CODt = 28 , CODs = 18, BODt = 10, SS = 18, NH3-N = 18, NO2--N =12 , NO3--N = 1.0, and P = 0.0 mg/L were obtained, and the average removal total efficacies were 93, 93, 90, 75, 72, 95, and 100%, respectively. Effluent qualities are far superior to the national effluent standards for domestic sewage in Taiwan.

This research investigated the seasonal and spatial distributions of picophytoplankton, including Prochlorococcus, Synechococcus and picoeukaryotes, in the northern South China Sea. Monitoring experiments including light intensity control and nutrient enrichment were conducted concurrently with on board sampling to examine factors affecting their cell densities dynamics. Quantification of cell numbers was carried out by flow cytometry. Averaged Synechococcus abundance in the South China Sea was 1¡Ñ104 cells ml-1, high in winter (1.37¡Ó0.30¡Ñ104 cells ml-1) and low in summer or fall (0.51¡Ó0.13¡Ñ104 cells ml-1 and 0.53¡Ó0.22¡Ñ104 cells ml-1, respectively). During a same season of the year, there was more Synechococcus in the shelf-slope region than in the basin. The cell density in summer, but not in winter, was significantly positively related to surface water nutrient concentration. Nutrient enrichment experiment carried out in winter also indicated that the growth of Synechococcus did not respond to addition of nitrate. On the other hand, Synechococcus seemed to prefer high illumination. In the light intensity experiment, Synechococcus collected from surface water grew better at 100% surface illumination than <100% light intensities. Synechococcus collected from deep water grew best at 30% and 18% of surface illuminations. Vertically, Synechococcus concentrated mostly in surface water with maximum cell number occurring at the surface or a few meters deep. Nutrient enrichment experiment in winter also showed that Synechococcus responded significantly to iron addition. Average cell density of picoeukaryotes was always less than 0.5¡Ñ104 cells ml-1, being high in winter (0.46¡Ó0.10¡Ñ104 cells ml-1) and low in summer or fall (0.15¡Ó0.02¡Ñ104 cells ml-1 and 0.19¡Ó0.03¡Ñ104 cells ml-1, respectively). Picoeukaryotes was always more concentrated in the shelf-slope region than in the basin, especially in winter when cell density in the shelf-slope region was 0.70¡Ó0.11¡Ñ104 cells ml-1. Although in winter picoeukaryotes was significantly positively related to surface water nutrient concentration, enrichments of nitrate or iron did not enhance their growth. Prochlorococcus had a cell density > 5.5¡Ñ104 cells ml-1 in the euphotic zone, and distributed as deep as 200 m. Light intensity monitoring experiment showed that Prochlorococcus from surface water grew better under high illumination than those from deep water and vice versa. Under 9% of surface illumination, deepwater Prochlorococcus population showed a positive growth, corresponding well with its deep distribution. Nutrient enrichment experiment conducted in winter showed that Prochlorococcus did not respond to enrichment of nitrate or iron.

China¡¦s water pollution problem has been experienced remediation by Chinese government numerous times; however, not much improvement is seen so far. In addition, Chinese government admits that algae, nutrient and other water pollution problems will not be eliminated in short period of time. Therefore, this study examined the current situation of China¡¦s water bodies as well as the sources of the pollution, and then compared with the measures taken to the problems by the Chinese government. It is found that city and household sewage are the major factors; nevertheless, they haven been less regarded. Therefore, the government should not only maintain the past results of remediation, but also emphasize on strengthening on constructions of sewage discharge facilities and ensuring capital provided for sewage treatment plants in order to control the most water pollution sources into China¡¦s water bodies.

Nahua reservoir is an important water resource for supplying drinking water to the Tainan area and a part of Kaohsiung in Taiwan. In recent years, Nanhua reservoir suffers eutrophication problems as many other reservoirs in Taiwan. In order to study eutrophication problems in reservoirs, a three-dimensional hydrodynamic and water quality model has been constructed using the FVCOM (Finite Volume Coastal Ocean Model) model to simulation the hydrodynamics, the nutrient dynamics and the phytoplankton growth in the Nanhua reservoir. The modeling of 3D hydrodynamics is the basic module dominating the circulation in the reservoir. The 3D eutro-dynamics are also calculated by the water quality module, which includes the dynamic variations of chlorophyll-a (Chl-a), dissolve oxygen (DO), carbon biological oxygen demand (CBOD), ammonia nitrogen (NH3-N), nitrate nitrogen (NO3-N), organic nitrogen (ON), phosphate (PO4) and organic phosphorus (OP). The model was first calibrated with the data measured in 2007 and, then, verified with the 2008 data. The model results are in reasonable agreement with the field measurements, both in the calibration and the verification phases. The water level variations are influenced by daily supply for the drinking water treatment and the inflows from the catchment and from Chiashian aqueduct during the dry season in spring. Nutrients are mainly carried into the reservoir through these routes. The residence time in the reservoir and the phytoplankton response with the nutrient loads are calculated. The model results indicated that phytoplankton growth is limited by low temperature and long residence time during the winter. The chlorophyll levels are getting higher from spring through out summer, which is due to enough sun lights and high nutrient loads carried by the catchment runoff. Surface temperatures are higher then the bottom layers causing stratification that worsen the eutrophication problems. Besides the comparisons by hydrodynamic and water quality parameters, the Carlson Trophic State Index (CTSI) has been calculated to categorize the eutrophication levels in the reservoir, which have shown good agreement with the CTSI calculated from EPA sampling data. Therefore, the model can be used as a tool for water quality management in the Nanhua reservoir.

The crisis of the water resources become a serious problem in recent years. Besides the global warming the problem mostly comes from quick population growth, intense industrial developments and low efficiency agricultural implementations. Biofilters are widely been used to either reduce pollution loads or also as a water conservation tool. And the vertical-flow biofilters act as a kind of bio-filter has gain the advantages of low maintenance, small footprint, greater capacities on both the hydraulic and organic loadings. It often used in to treat aquaculture wastewater for recycling during the filter stage. This study is, therefore, focusing on the bio-treatment processes to recycle the wastewater discharged from Chlorella production. Preserving water resources is one big issue of this study, Reuse the nutrients is another tough objective. For reusing the water with as much nutrients as possible and get the organic content off the water is the major target of the study. This study has been separated in two stages. A preliminary study has first been carried out in order to understand the Chlorella behaviors in more detail. Second phase includes the treatment tests with conventional activated sludge (AS) method and the bio-filters. The results have shown that ammonia is preferred by Chlorella as the nitrogen source. Light plays an important role on the treatment for removing algae activities. Aerobic digestion has shown better efficiency. AS can accept as high as 20% of daily input to the system volume, the system is not capable to bear more. While the biofilters, using either zeolite and LECA as the media, have shown satisfied results. When the hydraulic loading stay between 0.30 ~ 2.09 m3 m-2 day-1 to the system, the SS, COD, Chl-a removal rates can reach 90%, and more than 96% of total inorganic nitrogen (TIN) and 76% phosphorus can be preserved in the recycled water, respectively.