Nitrogen-doped carbon materials such as carbon nanotubes and graphene have garnered much interest due to their abilities to behave as electrocatalysts for reactions important in energy production (e.g. oxygen reduction) and biosensing (e.g. hydrogen peroxide reduction). Electrocatalytic properties of these materials have been attributed to enhanced electron transfer ability exhibited by surface nitrogen atoms compared to typical carbon structures. Screen-printing has been widely employed in the production of low-cost carbon-based electrodes for sensors and biosensors. Here, we develop nitrogen-doped screen-printed carbon (N-SPCE) electrodes for detection of hydrogen peroxide - an important analyte in biosensing. Conductive ink was formulated in the lab from nitrogen-doped graphite that was produced using a simple urea-based soft nitriding technique. N-SPCEs exhibited electrocatalytic activity towards hydrogen peroxide reduction, while SPCEs prepared from unmodified carbon showed no ability to electrocatalytically reduce H2O2. Amperometric detection of H2O2 using N-SPCEs at an applied potential of -0.4 V (vs. Ag/AgCl) displayed a wide linear range of 20 µM to 5.3 mM, and a low limit of detection (2.4 µM). These performance characteristics compare favorably to other electrodes for H2O2 sensing and indicate that the low-cost, easy-to-prepare N-SPCEs described here are promising platforms for the development of biosensors.

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Film does not exist without architecture. In every movie that has ever been made throughout history, the cinematic image of architecture is embedded within the picture. Throughout my studies and research, I began to see that there is no director who can consciously or unconsciously deny the use of architectural elements in his or her movies. Architecture offers a strong profile to distinguish characters and story. In the early days, films were shot in streets surrounded by architecture, and then they were projected on the exterior walls of buildings where the audience could come and watch. Here, I have studied elements such as light, time, space and matter- storymaking tools common to both Architect and Director. Light, for example, in architecture, is used as a strong theme by Louis I. Kahn, 'the most poetic of architects' in his Kimbell Art Museum. Similarly, Alfred Hitchcock, renowned filmmaker, utilizes light to distinguish the characters of his movies to create remarkable and sometimes horrifying scenes. This thesis aims to capture the cinematic movement of these elements in the building design. The project's program is a film museum and school that is sited in Southwest Washington DC. / Master of Architecture

Approved for public release, distribution is unlimited / Display technologies in the current market range from the simple and cheap incandescent bulb behind a graphic overlay to the upwardly expensive flat panel high definition plasma display. To provide a foundation of understanding for Light Emitting Polymers (LEP), samples were imaged in a scanning electron microscope. This was preformed to identify a potential method for answering questions on polymer charge mobility and diffusion mechanisms, which are currently unknown. Light Emitting Polymer (LEP) displays offer a viable alternative to the active matrix style, when an application calls for information to be sent in a simple visible format. By using the flexibility of the fabrication process, LEP displays can be applied to offer a low cost, lightweight, and durable means of communicating information during shipboard damage control and firefighting. A unique screen printing method was used in collaboration with Add-Vision, to produce a prototype that was designed, fabricated and tested for use in Naval shipboard firefighting evolutions. The application of the LEP technology to shipboard damage control was motivated by the experience gained from being both the Officer in Charge of a Naval Firefighting School and from time in the Fleet as a Damage Control Officer. / Lieutenant, United States Naval Reserve

Defective DNA repair capacity has been shown to be a common feature of cancer, and loss of function mutations in 'stability' genes that normally maintain the integrity of the genome may prove a key rate-limiting step in carcinogenesis. Since even genetically unstable cells require some repair functionality to maintain viability, these cancers likely exhibit an over-reliance on other DNA repair pathways for survival. Therapeutically targeting backup repair processes in such tumours represents a novel means by which to achieve selective tumour toxicity. Full exploitation of these synthetic lethal interactions will require an in-depth knowledge of the genetic basis of DNA repair in combination with an armoury of small molecule inhibitors of cellular targets. To this end, we have designed, optimised and run two high-throughput cell-based screens to identify genes and small molecules that can modulate mismatch repair (MMR) activity. Key to these screening strategies are the resistance of cells with dysfunctional MMR to a range of cytotoxic drugs, including the alkylating agent N-methyl-N'-nitro-N-nitrosoguanidine (MNNG). By exploiting this MMR-dependent toxicity we have assayed for siRNA and small molecules that permit the survival of MNNG-treated MMR-proficient cells to levels comparable to MMR-deficient cells, and which therefore represent putative MMR modulating agents. A screen of 571 siRNA for gene depletions that reduce MNNG sensitivity by at least two population standard deviations identified 10 genes of potential interest, and included the four canonical MMR genes, MSH2 (2.87 ± 0.28 (Z ± SE)), MSH6 (4.87 ± 0.06), MLH1 (3.42 ± 0.43) and PMS2 (3.36 ± 0.44). TDG represented an unexpected hit that decreased MNNG sensitivity by 2.55 ± 0.04 population standard deviations. However, clonogenic survival experiments found TDG depletion to be contextual synthetic lethal within an MMR-null background when treated with MNNG, reducing HCT116 clonogenicity by 37% (p < 0.001). Moreover, TDG knockdown increased the number of 53 binding protein 1 (53bp1) foci in MMR-proficient cells by 40% and MMR–deficient cells by 27% following MNNG exposure (p < 0.001). Combined with a failure to replicate the primary screen result, the role for TDG in the response to MNNG could be explained solely through its established role as a member of the base excision repair pathway. A second screen of the NCI Diversity I and II small molecule libraries (n=1786) was conducted to identify putative MMR inhibitors. Subsequent analysis revealed NSC197049 to increase cellular viability of MNNG treated cells by 3.60±0.32 population standard deviations and was successfully validated as a hit. Co-treatment of NSC197049 with MNNG conferred dose-dependent chemoprotection independently of MMR status and cell line, an effect that was lost if NSC197049 was pre- or post-treated. The protection was associated with a reduction in MNNG-dependent 53bp1 foci of 60% in MMR proficient cells and 15% in MMR deficient cells (p < 0.001), together with a marked reduction of > 80% in subG1 content at 48 hours post-MNNG that was independent of MMR status. Interestingly, the characteristic G2/M arrest of MNNG-treated MMR-proficient cells remained intact (~40% arrested). Taken together, these observations are not consistent with NSC197049 acting as an inhibitor of MMR. Dithiolthiones have been described as chemoprotective agents that induce antioxidant defences, whilst we have found NSC197049 phenocopies known antioxidants ascorbic acid and glutathione in protecting against MNNG-induced toxicity. NSC197049 may therefore act by bolstering cellular antioxidant defences. The precise mechanism may be novel, since the proto-typical dithiolthione, Oltipraz, failed to be protective in this study. In summary, we have confirmed that MMR is the primary determinant of MNNG sensitivity, and found that TDG is unlikely to be involved in MMR. We have also identified a novel chemoprotective small molecule that is unlikely to represent an MMR inhibitor, but that might be useful in cancer chemoprevention.

While the MPAA’s Classification and Ratings Administration – or CARA – has generally expanded the freedoms of filmmakers since its 1968 inception, the economic failure of the NC-17 rating has led to substantial inconsistencies in the rating system. Because of the CARA model, filmmakers have been able to probe the extremes of violence under the R rating while they have been unable to do the same for screen sexuality. Through the NC-17 rating, CARA has been able to repress non-pornographic sexual portrayals by rating a given film NC-17, thus forcing contractually obligated directors to make edits that are sometimes inconsistent and arbitrary. Though cinema used to have significant thematic and visual freedoms over television, NC-17 level paid cable programming has surpassed what is allowed under CARA’s R-rating, allowing for more complex and mature viewpoints on sexuality than is currently allowed to regularly reach film audiences.

Photorealism is what visual effects are all about most of the time. This report entails digital compositing and studio lighting, in relation to Chroma key film material, aimed to give a photorealistic impression.    One of the identified problems in this report is that compositors may get Chroma key footage where the lighting is done poorly, which means a lot of extra work for the compositors and it might even make it impossible to create the desired end result.    Another problem recognized is that the knowledge that these professions possess is often tacit, not available in texts or even functionally defined.    Considering these problems, the purpose of this report is to articulate and try the tacit knowledge found in respect to these research questions: Which factors can alter the photorealistic impression of filmed Chroma key material? To what extent can different factors be altered in the compositing process, for a photorealistic result? How can a photorealistic result from composited Chroma key material be enabled and facilitated, with focus on studio lighting?       Methods used to answer these questions are interviews with compositors, a case study of a small video production, and the production of video clips, including studio lighting and compositing.    While professionals often write about the importance of consistency in image characteristics between different element that are composited together, this report defines which specific features that ought to be consistent, for a photorealistic result.    Further findings are focused on the limitations of the compositor; i.e. the features that are possible to manipulate and the features that have to be set correctly when filming in the studio, to enable a photorealistic outcome. Nonetheless, the main focus will be on the features of lighting set in the Chroma key film studio.    In fact, there are many features that are crucial for enabling and facilitating the compositing of a photorealistic end product. While some of the findings are new, others confirm what has already been presented.

Previous studies have shown that screens may be utilized efficiently for dissipating the energy of water. For the present study, a series of experimental works are executed to investigate the tailwater and multiple screen effects on the energy dissipation through screens. Water flowing beneath a sliding gate is used to simulate the flow downstream of a hydraulic structure. In the present study, one double screen and two double screen arrangements with porosity of 40% and inclination angle of 90 degree is used. A tailwater gate structure is used to adjust the tailwater depth. The major parameters for the present study are upstream flow depth, tailwater gate height, location of the screen together with the supercritical upstream flow Froude number for a range covering from 5.0 to 22.5. The gate opening simulating a hydraulic structure is adjusted at heights of 2 cm and 3 cm during the study. The results of the experiments show that the tailwater depth has no significant additional contribution on the energy dissipation, whereas multiple screen arrangement dissipates more energy as compared to one double screen arrangement.