Würzburg, Univ., Diss., 2008.

Texte remanié de: Dissertation--Theologische Fakultät--Kiel--Christian-Albrechts-Universität, 1990. / Bibliogr. p. 596-614. Bibliogr. des oeuvres d'Oskar Pfister p. 575-595.

Oskar (Osk) is a posterior body-patterning determinant in Drosophila melanogaster and is highly concentrated at the posterior pole of the oocyte. osk mRNA is translationally repressed until it reaches the posterior of the oocyte where Osk protein is made. Bruno (Bru) represses translation during osk mRNA localization by direct binding, but how Bru-mediated repression is relieved at the posterior of the oocyte is unknown. Two types of Bru protein interactions are implicated in repression of osk: Bru-Cup interaction and Bru dimerization. By mapping the Bru domains that are important for these interactions, I found that the amino-terminal domain of Bru contributes to both interactions, and deletion of this domain caused a defect in translational repression. However point mutations, within the amino-terminal domain, that disrupt both types of interaction in vitro did not interfere with translational repression in vivo. The difference may be due to other factors stabilizing the Bru-Cup interaction in vivo, as the mutant Bru still associates with Cup in vivo. My work supports the model of repression that relies on Bru interaction with Cup. I also build a new model in which Bru dimerization promotes translational activation of osk, based on my unexpected results: dimerization-defective Bru only weakly accumulated Osk::GFP fusion protein encoded by an osk::GFP reporter RNA bearing a Bru-binding region, while dimerization-competent Bru showed the opposite effect. This suggests that dimerization may contribute to switching Bru from a repressor to an activator, with dimerization controlled via a post-translational modification. Consistent with this, I found that a small fraction of Bru in ovaries is phosphorylated. PKA is a positive regulator of osk expression and phosphorylates Bru in vitro. To test if PKA regulation of osk is mediated through Bru, I examined the effect of altering PKA activity on Bru phosphorylation and Bru-mediated repression. Modulating PKA activity caused small, yet detectable changes in Bru phosphorylation and Bru-dependent translational repression using an osk::GFP reporter. However, while the studies with Bru mutants suggest that phosphorylation promotes repression by Bru, these studies argue for a role in promoting activation. Further work will be required to explain these phenomena. / text

Inaug. _ Diss.: Philosophische Fakultät: Münster-Westfalen: 1973. _ Bibliogr. p. 311-330.

This thesys focuses on the communication of the Oskar company, Oskar-Vodafone and later Vodafone. In the first part is the description of the below the line part of commercial communications. In the second part the main focus is on the blow the line communication and its changes of the Oskar company to Vodafone. The focus is on the building up process of the brand and company identity. The last part is the summary of the customer experience with theese companies and reasons for the changes and behavior in that relationship.

This entry briefly examines the major contributions of Paul Oskar Kristeller to the intellectual history of the Italian Renaissance. It focuses on Kristeller’s two most significant cataloguing and editorial projects in addition to the continuing impact of Kristeller’s own scholarship on the history of Renaissance thought.

Oskar (Osk) is a posterior body patterning determinant in Drosophila melanogaster oocytes. oskar (osk) mRNA is translationally repressed until it reaches the posterior of the oocyte where Osk protein accumulates. Translational repression of osk prior to posterior localization is mediated by the RNA binding protein, Bruno (Bru). To better define Bru binding sites, I performed in vitro selections using full length Bru and the fragments containing either the first two RRMs (RRM1+2) or the third RRM (RRM3+). The aptamers from the final round from each of the selections produced a multitude of overrepresented primary sequence motifs. Examples of each of these motifs were found in the 3’UTRs of the mRNAs that Bru is known to regulate during oogenesis. GFP reporter transgenes under the control of the UAS-Gal4 expression system were constructed with each class of the binding sites within the reporter transgenes’ 3’UTRs to test the motifs’ ability to repress the reporters in vivo. In a wildtype background, the GFP reporters containing the binding sites were translationally repressed. In the aret mutant background, the GFP levels of the repressed GFP reporters increased with reduced Bru activity, suggesting the transgenes’ repression is mediated by Bru. Three of the motifs isolated in the in vitro selections reside in the AB and C regions of the osk 3’UTR, and the three classes of sites were mutated in the AB and C regions. The mutated AB and C regions were used to assay for a reduction of Bru binding affinity for the mutant RNAs. Additionally, the mutations were incorporated into an osk genomic transgene that was introduced into an osk RNA null as well as an Osk protein null background. The mutations reduced Bru binding to the AB and C regions. The transgenes containing the mutated Bru binding sites could not fully rescue the osk RNA null phenotype but can fully rescue the Osk protein null phenotype, suggesting an osk transcript can regulate other osk mRNAs in trans. / text

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Denna självständiga studie behandlar en musikalisk djupdykning i den första satsen av Oskar Böhmes trumpetkonsert op.18 i f-moll. Genom en musikteoretisk, grafisk och praktisk analys av satsen fördjupar jag mig i musiken på ett annat sätt än att som musiker bara spela den och kan därigenom förhoppningsvis ta till mig musiken på ett djupare plan. / <p>Bilaga: 1 CD</p><p>Oskar Böhmes trumpetkonsert op.18</p><p>Filip Svensson- trumpetsolist</p><p>Ejmunds Sinfonietta</p>