Thesis (Ph. D.)--University of Missouri-Columbia, 1998. / Typescript. Vita. Includes bibliographical references (leaves 110-136). Also available on the Internet.

Source: Masters Abstracts International, Volume: 46-01, page: 0335. Adviser: Alanna Morrison. Includes bibliographical references.

Source: Masters Abstracts International, Volume: 46-04, page: 2093. Advisers: Xianglin L. Du; Karen H. Lu. Includes bibliographical references.

Thesis (M.S.)--University of Missouri-Columbia, 2004. / Typescript. Vita. Includes bibliographical references (leaves 101-110). Also available on the Internet.

Thesis (Ph. D.)--West Virginia University, 2006. / Title from document title page. Document formatted into pages; contains ix, 224 p. : ill. (some col.). Vita. Includes abstract. Includes bibliographical references.

Thesis (PhD)--University of Mastericht, the Netherlands, 2007. / Includes bibliographical references.

Les tumeurs malignes rares de l'ovaire représentent moins de 8% des tumeurs ovariennes de l'adulte. Il s'agit essentiellement des tumeurs germinales de l'ovaire et des tumeurs du stroma et des cordons sexuels de l'ovaire (tumeurs de la granulosa et tumeurs de Sertoli-Leydig). En 2002, du fait de l'extrême rareté de ces tumeurs, un site internet français (www.ovaire-rare.org) a été créé dans le but d'informer les patientes, de créer un forum de discussion, d'actualiser les informations scientifiques, d'inclure les données cliniques et biologiques des patientes et de collecter les blocs de tumeurs. Ce travail coopératif a pour but de standardiser la gestion des tumeurs ovariennes non épithéliales, et d'accumuler les données cliniques et biologiques. Cet observatoire nous a permis de regrouper plus de 200 tumeurs sur lequel repose notre travail. Des mutations des gènes FOXL2 et DICER1 ont été récemment décrites respectivement dans les tumeurs de la granulosa et les tumeurs de Sertoli-Leydig. Nous avons montré l'importance de la recherche de ces mutations pour le diagnostic et le pronostic de ces tumeurs. Nous avons également élaboré un nouvel arbre décisionnel, incluant ces nouveaux outils, et qui a permis de corriger plus de 20% des diagnostics initiaux. Au niveau moléculaire, nous avons analysé l'impact de la mutation C134W de FOXL2 sur le développement des tumeurs de la granulosa. De façon très intéressante, nous montrons une expression préférentielle de la protéine anti-apoptotique Bfl-1 (famille Bcl-2) dans les cellules exprimant FOXL2 sous sa forme mutée. L'expression de Bfl-1 est induite dans les lignées surexprimant la forme mutée de FOXL2 et confère aux cellules une résistance au stress apoptotique. De même, l'inhibition de l'expression de Bfl-1 restaure la sensibilité à l'apoptose induite par des stress apoptotiques. L'ensemble de nos résultats montre que Bfl-1 est une cible transcriptionnelle de FOXL2 et qu'il constitue une cible thérapeutique très intéressante pour ces tumeurs. En conclusion, nos travaux ont permis d'améliorer nos connaissances sur les tumeurs rares de l'ovaire, en terme de pronostic et de diagnostic, mais aussi d'entrevoir de nouvelles stratégies thérapeutiques / Rare malignant ovarian tumors represent less than 8% of ovarian tumors in adults. It is essentially germ cell tumors of the ovary and stromal tumors and sex cord ovarian (granulosa cell tumors and Sertoli-Leydig cell tumors). In 2002, due to the extreme rarity of these tumors, a French website (www.ovaire-rare.org) was created to inform patients, create a discussion forum, update scientific information, to include clinical and laboratory data of patients and collect blocks of tumors. This collaborative work has the purpose to standardize the management of nonepithelial ovarian tumors, and accumulating clinical and laboratory data. The observatory has allowed us to consolidate more than 200 tumors on which our work is based. Mutations of FOXL2 and DICER1genes have recently been described respectively in the granulosa cell tumors and Sertoli-Leydig tumor. We have shown the importance of research of these mutations for the diagnosis and prognosis of these tumors. We also developed a new decision tree, including these new tools, and has corrected more than 20% of initial diagnoses. At the molecular level, we analyzed the impact of the FOXL2 C134W mutation on the development of granulosa cell tumors. Very interestingly, we show preferential expression of anti-apoptotic protein Bfl-1 (Bcl-2A1) in cells expressing FOXL2 mutated form. Bfl-1 expression is induced in the lines overexpressing the mutated FOXL2 and confers resistance to apoptotic cells form stress. Similarly, inhibition of expression Bfl-1 restores sensitivity to apoptosis induced by the apoptotic stress. All of our results show that Bfl-1 is a transcriptional target of FOXL2 and it is a very interesting therapeutic target for these tumors. In conclusion, our work has improved our knowledge about rare ovarian tumors in terms of prognosis and diagnosis, but also opens up new therapeutic strategies

Transgenesis of silkworms has great potential for the development of silk with new properties as well as for the preparation of recombinant proteins for the use in biomedicine. Zabelina et al. (2015a) showed that transgenesis of parthenogenetic silkworms facilitates the selection and maintenance of transgenic homozygotes with stabile transgene insertions. However, the efficiency of transgenesis was less than 2 % compared to 60 % in the standard, non-parthenogenetic silkworms. The purpose of the present research was to explore the cause of this difference. Since transgenesis is normally performed at 25 °C but in the parthenogenetic silkworms at 15 °C (3 days incubation at this temperature is part of the protocol for the induction of parthenogenetic development), we assumed that the eggs incubated at 15 °C might have been injected with the DNA construct at unsuitable time. The work was therefore focused on the rate of embryogenesis at 15 °C in the eggs treated in different ways. Intensive cleavage of the control eggs (strain K23) was observed at 12 h after oviposition at 25 °C and between 24 and 36 h at 15 °C. The transgenesis of parthenogenetic silkworms is also complicated by the embryonic diapause. In the current work, diapause was suppressed by implanting PK1 ovaries into the non-parthenogenetic male hosts K23. Parthenogenetic development was activated by the heat shock in the chorionated eggs dissected from the implants. No cleavage was detected at 12 h after the acti-vation and nearly complete blastoderm was found at 48 h. In respect to the course of cleavage at 15 °C, transgene injection 24 h after the activating heat shock can be recommended. The eggs from endogenous ovaries of the K23 females, which also contained the implants of the PK1 ovaries, acquired partial capacity of parthenogenesis. Low rate of embryogenesis was also found in the transgenic clone VTG1. Current results suggest that more research is needed to understand and possibly explore differences in the rate of silkworms exposed to different treatments.

Submitted by Diogo Misoguti (diogo.misoguti@gmail.com) on 2016-07-07T18:49:46Z No. of bitstreams: 1 cp078451.pdf: 1963747 bytes, checksum: 8df763273b9205acc5055fd0cbc3d714 (MD5) / Approved for entry into archive by Diogo Misoguti (diogo.misoguti@gmail.com) on 2016-07-07T18:50:39Z (GMT) No. of bitstreams: 1 cp078451.pdf: 1963747 bytes, checksum: 8df763273b9205acc5055fd0cbc3d714 (MD5) / Made available in DSpace on 2016-07-07T18:50:39Z (GMT). No. of bitstreams: 1 cp078451.pdf: 1963747 bytes, checksum: 8df763273b9205acc5055fd0cbc3d714 (MD5) Previous issue date: 2008 / Objetivo: Avaliar, do ponto de vista histológico, o efeito do transplante de tecido adiposo sobre o útero e os ovários de camundongas obesas e anovuladoras. Métodos: Foram utilizadas camundongas B6.V-Lepob/J com 2 e 3 meses de idade, subdivididas em grupos experimentais: 1.Controle (B6.V-Lepob/J fêmeas, com peso normal); 2.Obesidade (B6.V-Lepob/J fêmeas, obesas); 3.Obesidade + Transplante de tecido adiposo de camundongas doadoras com peso normal após 7 dias (B6.V-Lepob/J fêmeas e obesas, que receberam aproximadamente 1g de tecido adiposo de camundongas com peso normal isogênicas); 4.Obesidade + Transplante de tecido adiposo de camundongas doadoras com peso normal após 15 dias (B6.V-Lepob/J fêmeas e obesas, que receberam aproximadamente 1g de tecido adiposo de camundongas com peso normal isogênicas); 5.Obesidade + Transplante de tecido adiposo de camundongas doadoras com peso normal após 45 dias (B6.V-Lepob/J fêmeas e obesas, que receberam aproximadamente 1g de tecido adiposo de camundongas com peso normal isogênicas). Após 7, 15 e 45 dias do transplante, os animais foram sacrificados. Foram verificados o peso corporal e a glicemia, além da análise histológica dos úteros e dos ovários (H/E). Resultados e Conclusões: O grupo das obesas (n=6) apresentou elevação no peso corporal (52,0± 1,3*) e na glicemia (418,4±33,0*) em relação ao controle (24,0±0,7 e 100,2±2,2, respectivamente, n=5). Os transplantes após 7 dias (n=5) e 15 dias (n=9) foram eficazes na redução da glicemia (390,2±34,0 e 270,0±38,0*, pré e pós-Tx 7 dias; e 460,0±31,4 e 125,0±8,4*, pré e pós-Tx 15 dias, respectivamente), mas não modificaram o peso corporal. Porém, após 45 dias de transplante (n=7) houve redução da glicemia (467,0±28,0 e 209,0±18,0*, pré e pós-Tx, respectivamente), assim como tendência à diminuição no peso corpóreo observado pré e pós-Tx (50,4±2,4 e 44,0±3,3). Valores adotados para *p< 0,0001. A histologia revelou que, após 7 e 15 dias de Tx, os animais apresentavam ovários semelhantes aos das obesas, incluindo presença de inúmeros folículos, interstício abundante e ausência de corpos lúteos. Nos úteros notou-se intensa estimulação hormonal e glandular, mitoses, raros eosinófilos e epitélio superficial íntegro e sem renovação, sugerindo que tempos precoces de Tx são incapazes de reverter tais alterações. Entretanto, no grupo Tx 45 dias foram visualizados úteros praticamente iguais aos do controle, com grande número de leucócitos e remodelação do epitélio superficial, além de ovários com reduzida quantidade de folículos e presença de corpos lúteos, indicando ovulação. As camundongas B6.V-Lepob/J obesas apresentam formações polimicrocísticas e estroma abundante, mimetizando os achados ovarianos das pacientes anovuladoras. O Tx de tecido adiposo, a longo prazo, parece ter papel na diminuição da obesidade, mostrando-se eficiente na reversão da hiperglicemia e das alterações ovarianas e uterinas, presentes nas camundongas obesas, restabelecendo a ovulação. / Objective: To assess from the histological point of view the effect of adipose tissue transplantation onto the uterus and ovaries of obese, anovulating mice.en Method: B6.V-Lepob/J mice were used and the following experimental groups evaluated: 1.Control group (B6.V-Lepob/J female, average weight); 2.Obesity (B6.V-Lepob/J female, obese); 3.Obesity plus adipose tissue transplant from mice donors which were average weight after 7 days (B6.V-Lepob/J obese females which received adipose tissue from thin, isogenic mice); 4.Obesity plus adipose tissue transplant from mice donors which were average weight after 15 (B6.V-Lepob/J obese females which received adipose tissue from thin, isogenic mice); 5.Obesity plus adipose tissue transplant from mice donors which were average weight after 45 days (B6.V-Lepob/J obese females which received adipose tissue from thin, isogenic mice);. Within 7, 15 and 45 days of the transplant the animals were sacrificed. The age range was between 2 and 3 months old. In addition to the histological analyses of uteri and ovaries (H/E), these animals’ corporal weight and glycemia were verified. Results and Conclusion: The obese group (n=6) presented with elevation in corporal weight (52,0 ± 1,3*)and glycemia (418,4± 33,0*) in relation to the control group (24,0 ± 0,7 and 100,2± 2,2 respectively, n=5). The transplants after 7 days (n=5) and 15 days (n=9) were effective in reducing glycemia (390,2 ± 34,0 and 270,0 ± 38,0*, 7 days pre and post transplant; and 460,0 ± 31,4 and 125,0 ± 8,4*, 15 days pre and post transplant respectively), but not in changing corporal weight. However, within 45 days of the transplant (n=7) there was a reduction in glycemia (467,0 ± 28,0 and 209,0 ± 18,0*, pre and post- transplant respectively), as well as a tendency to reduce corporal weight observed pre and post transplant (50,4 ±2,4 and 44,0 ± 3,3). Values used for *p< 0,0001. Histology revealed that within 7 and 15 days of the transplant, the animals presented with ovaries similar to those in the obese group, containing countless follicles, abundant interstice and absence of corpora lutea. The uteri revealed intense hormonal and glandular stimulation, mitosis, scarse eosinophils and the superficial epithelium whole, without renovation, indicative of the inability of short term transplantations to reverse such alterations. In the 45-day-group, however, the uteri looked very much the same as those of the control group, with a great number of leucocytes and remodellation of the superficial epithelium, as well as ovaries with a reduced amount of follicles and presence of corpora lutea, suggesting ovulation. The obese B6.V-Lepob/J mice present with polymicrocystic formation and abundant oestrone, mimicking the ovarian findings of the anovulating patients. The long term adipose tissue transplantation, on the other hand, seems to play a part in reducing obesity, proving to be efficient in reversing hypoglycemia and the ovarian and uterine alterations present in obese mice, re-establishing ovulation

Made available in DSpace on 2014-06-11T19:31:32Z (GMT). No. of bitstreams: 0 Previous issue date: 2011-11-03Bitstream added on 2014-06-13T21:02:42Z : No. of bitstreams: 1 palacio_ep_dr_botfm.pdf: 758081 bytes, checksum: 31ee7906cb02d270857301e0c39e8dc2 (MD5) / Universidade Estadual Paulista (UNESP) / Com o objetivo de se investigar as repercussões clínicas, densitométricas, biomecânicas e microtomográficas do ácido zoledrônico sobre o tecido ósseo de ratas osteoporóticas, após menopausa induzida por ooforectomia bilateral, foram estudadas, prospectivamente, 80 ratas da linhagem Wistar (Rattus novergicus albinus), adultas e virgens. Com trinta dias de vida, os animais foram aleatorizados, pelo método de sorteio de envelopes opacos e lacrados, em dois grupos, segundo o tipo de procedimento cirúrgico a que seriam, imediatamente, submetidos: grupo O (ooforectomia bilateral) (n=40) e grupo S (cirurgia sham) (n=40). No 30º dia de pós-operatório, os animais foram novamente sorteados e divididos em quatro subgrupos, de acordo com a administração, intraperitoneal, de 0,1mg/kg de ácido zoledrônico ou água destilada, totalizando os quatro grupos de estudo: grupo OAZ (ooforectomizado com ácido zoledrônico) (n=20), grupo OAD (ooforectomizado com água destilada) (n=20), grupo SAZ (cirurgia sham com ácido zoledrônico) (n=20) e grupo SAD (cirurgia sham com água destilada) (n=20). Todos os animais foram mensalmente pesados ao longo do experimento. A eutanásia ocorreu com dose letal de pentobarbital sódico 3% (80 mg/kg), intraperitonealmente, em dois momentos, após a administração das substâncias: nove meses (Momento 1) e 12 meses (Momento 2). Para a análise do peso, foi considerada a sua curva evolutiva ao longo de todo o experimento. Os fêmures direitos foram utilizados para o estudo biomecânico, analisando-se as variáveis: carga máxima (N), limite de elasticidade (N) e coeficiente de rigidez (N/mm). Os fêmures esquerdos foram utilizados para o estudo densitométrico (mmAl) e para o estudo microtomográfico... / The objective of this study was to prospectively investigate the clinical, densitometric, biomechanical and microtomographyc effects of zoledronic acid in the femurs of 80 female Wistar rats (Rattus norvegicus albinus), with osteoporosis due to bilateral oophorectomy. With the age of thirty days, the animals were randomized into two groups, according to the surgical procedures: bilateral oophorectomy (group O) (n=40) and sham surgery (group S) (n=40). On the 30th postoperative day, the subjects were randomly divided into four groups, according to the intraperitoneal administration of 0.1 mg/kg of zoledronic acid or distilled water: group OAZ (ovariectomized group with zoledronic acid) (n=20), group OAD (ovariectomized group with distilled water) (n=20), group SAZ (sham group with zoledronic acid) (n=20) and group SAD (sham group with distilled water) (n=20). All animals were monthly weighted. The animals were euthanized with a lethal intraperitoneal dose of sodium pentobarbital 3% (80 mg/kg) after a follow up period of nine months (n=40) and 12 months (n=40). For the analysis of weight, it was considered the weight curve throughout the experiment. The right femurs were used for biomechanical tests, analyzing the maximum load (N), the yield point (N) and stiffness coefficient (N/mm). The left femurs were used for both, densitometric (mmAl) and microtomographyc tests, getting the bone volume (mm3), the trabecular number (mm-3), the trabecular diameter (mm) and intertrabecular separation (mm). The statistical analysis was performed through the analysis of variances and complemented with the multiple averages comparison tests, for a 2 x 2 x 2 factorial project (two substances, two types of surgical procedures and two periods of euthanasia). It was used an entirely casual model... (Complete abstract click electronic access below)