Submitted by Rodrigo Martins Cruz (rodrigo.cruz@ufvjm.edu.br) on 2016-01-05T17:37:15Z No. of bitstreams: 2 vitor_oliveira_rodrigues.pdf: 1888771 bytes, checksum: 2a13199fad989883502026feb4526f36 (MD5) license_rdf: 22974 bytes, checksum: 99c771d9f0b9c46790009b9874d49253 (MD5) / Approved for entry into archive by Rodrigo Martins Cruz (rodrigo.cruz@ufvjm.edu.br) on 2016-01-05T18:03:14Z (GMT) No. of bitstreams: 2 vitor_oliveira_rodrigues.pdf: 1888771 bytes, checksum: 2a13199fad989883502026feb4526f36 (MD5) license_rdf: 22974 bytes, checksum: 99c771d9f0b9c46790009b9874d49253 (MD5) / Made available in DSpace on 2016-01-05T18:03:14Z (GMT). No. of bitstreams: 2 vitor_oliveira_rodrigues.pdf: 1888771 bytes, checksum: 2a13199fad989883502026feb4526f36 (MD5) license_rdf: 22974 bytes, checksum: 99c771d9f0b9c46790009b9874d49253 (MD5) Previous issue date: 2014 / Funda??o de Amparo ? Pesquisa do estado de Minas Gerais (FAPEMIG) / Coordena??o de Aperfei?oamento de Pessoal de N?vel Superior (Capes) / A cultura do girassol (Helianthus annuus L.) ? de grande import?ncia econ?mica, devido seu uso na produ??o agr?cola para a extra??o do ?leo, considerado, dentre os ?leos vegetais, como um dos ?leos de melhor qualidade nutricional al?m de possuir alta qualidade para a produ??o do biodiesel. Esta cultura apresenta um alto grau de adaptabilidade ?s varia??es edafoclim?ticas, ? tolerante ao d?ficit h?drico al?m de apresentar menor custo de produ??o que as outras oleaginosas, antevendo-se uma boa e nova op??o de renda aos produtores brasileiros. A propaga??o do girassol ? feita exclusivamente via semente e por isso ? importante a obten??o de sementes de alta qualidade gen?tica, f?sica, fisiol?gica e sanit?ria. Dentre os fatores que afetam a difus?o da cultura de girassol destaca-se a presen?a de doen?as causadas por v?rus bact?rias e fungos, j? que o girassol ? hospedeiro de uma vasta gama de microorganismos e cuja maioria pode ser transmitida via semente. Como alternativa de substitui??o da aplica??o de produtos quimicos, o oz?nio tem sido usado no controle de insetos-praga e de fungos em gr?os armazenados, al?m de ser utilizados em diversos outros seguimentos na elimina??o de microorganismos. Diante do exposto, objetivou-se avaliar a efici?ncia do oz?nio no controle de pat?genos em sementes de girassol. Foram utilizados tr?s lotes de sementes de girassol da cultivar Embrapa 122 de tr?s peneiras (3,5 mm; 4,0 mm e 5,5 mm). Para caracteriza??o do perfil dos lotes realizou-se a determina??o do grau de umidade, primeira contagem de germina??o, germina??o, ?ndice de velocidade de germina??o, emerg?ncia, estande inicial, ?ndice de velocidade de emerg?ncia e sanidade (incid?ncia e severidade). Para avaliar a a??o do oz?nio na sanitiza??o do girassol as sementes foram mantidas em ambiente rico em oz?nio pelos per?odos de 20 minutos, 60 minutos, 120 minutos e sem ozoniza??o (controle). Ap?s o tratamento com oz?nio, al?m dos testes citados anteriormente foram tamb?m realizados os testes de envelhecimento, tetraz?lio e sanidade. Conclui-se que o tratamento de sementes de girassol, cultivar Embrapa 122 com oz?nio na concentra??o de 1741 ppmv (0,24 g/h), por 60 minutos, reduz a popula??o f?ngica de Alternaria sp., Fusarium sp., Aspergillus sp. e Penicillium sp sem afetar o seu potencial fisiol?gico. / Disserta??o (Mestrado) ? Programa de P?s-Gradua??o em Produ??o Vegetal, Universidade Federal dos Vales do Jequitinhonha e Mucuri, 2014. / ABSTRACT The culture of sunflower (Helianthus annuus L.) is of wide economic importance because of its use in agricultural production for the extraction of oil, considered among the vegetable oils, as the oils of better nutritional quality besides having high quality production biodiesel This culture has a high degree of adaptability to changes in climate and soil, is tolerant to drought and presents lower production cost than other oilseeds, foreseeing a good new income option for Brazilian producers. The spread of sunflower is made exclusively by seed and so it is important to obtain high quality seed genetics, physical, physiological and sanitary. Among the factors affecting the diffusion of the sunflower crop stands the presence of diseases caused by viruses fungi and bacteria as the host sunflower is a wide range of microorganisms and that the large majority may be transmitted via seed. Alternatively replacement of applying chemicals, ozone has been used for controlling insect pests and fungi in stored grains, and are used in many other segments in eliminating microorganisms. Given the above, the objective was to evaluate the efficiency of ozone to control pathogens on sunflower seeds. Three lots of sunflower cultivar Embrapa 122 three sieves (3.5 mm, 4.0 mm and 5.5 mm) were used. To characterize the profile of lots was held to determine the degree of moisture content, the first germination, germination index, germination rate, emergence, initial stand, speed index and emergency health (incidence and severity). To evaluate the effect of ozone sanitization in sunflower seeds were kept in ozone-rich environment for periods of 20 minutes, 60 minutes, 120 minutes and without ozonation (control). After the treatment with ozone, besides the above-mentioned tests were also conducted aging tests, tetrazolium health. We conclude that treatment of sunflower seeds, Embrapa 122 with ozonated water 1741 ppmv (0.24 g/h) for 60 minutes reduces the fungal population of Alternaria sp., Fusarium sp., Aspergillus sp. and Penicillium sp without affecting their physiological potential.

Submitted by PPG Odontologia (odontologia-pg@pucrs.br) on 2018-03-13T18:14:07Z No. of bitstreams: 1 GUILHERME_PIVATTO_LOUZADA_DIS.pdf: 4084164 bytes, checksum: 92327e9e0d6d6a7868235af68dc557f1 (MD5) / Approved for entry into archive by Tatiana Lopes (tatiana.lopes@pucrs.br) on 2018-03-21T11:56:19Z (GMT) No. of bitstreams: 1 GUILHERME_PIVATTO_LOUZADA_DIS.pdf: 4084164 bytes, checksum: 92327e9e0d6d6a7868235af68dc557f1 (MD5) / Made available in DSpace on 2018-03-21T12:03:36Z (GMT). No. of bitstreams: 1 GUILHERME_PIVATTO_LOUZADA_DIS.pdf: 4084164 bytes, checksum: 92327e9e0d6d6a7868235af68dc557f1 (MD5) Previous issue date: 2018-01-12 / Coordena??o de Aperfei?oamento de Pessoal de N?vel Superior - CAPES / Objective: The present study aimed to evaluate the effects of systemic ozonotherapy on bone remodeling of critical defects in the calotte of rats, with and without a xenograft presence, using an animal model of immunosuppression induced by corticosteroids. Methods: Sixty male Wistar rats (180-220 g), distributed in 8 experimental groups (N = 8 / group) were used. For corticosteroid therapy (groups II, IV, VI and VIII), the animals received dexamethasone (1 mg / kg; i.p.), one injection every 48 h, starting two weeks before the surgical procedures, extending to euthanasia. Control animals (groups I, III, V and VII) received saline solution (10 ml / kg, i.p.) at the same time intervals. After two weeks of corticosteroid therapy, the animals were anesthetized with ketamine and xylazine (100 and 10 mg / kg, i.p.) to produce two critical defects in the parietal bones of the skull cap (5 mm diameter). The defects were filled with clot (groups I, II, V and VI) or Bio-Oss x xenograft (Geistlish Biomaterials, Germany) (groups III, IV, VII and VIII). In all groups, the right defect was covered by a Bio-Gide? collagen membrane (Geistlish Biomaterials, Germany). For systemic ozono-therapy, animals of groups V, VI, VII and VIII received an application of ozone (0.7 mg / kg, i.p.) every day for 7 days, starting immediately after surgery. Four weeks after the defects were created, the animals were euthanized, and the skull caps were collected for histological evaluation of the bone neoformation with hematoxylin and eosin (HE) staining. The protocols were approved by the Ethics Committee on the Use of Animals (7691). Qualitative histological analyzes were performed based on the pattern of connective tissue formation around the defect, existence of inflammatory cells in the region, aspect of bone trabeculated, osteoblastic activity around the bone matrix and existence of graft particles in the region. Results: Groups V and VII presented greater bone areas along the surface of the defect compared to VI and VIII, because of the potential effects of ozone on bone remodeling, minimizing negative interference in the glucocorticoid bone microarray. The presence of the xenografts groups III, IV, VII, VIII, provided the covering of an extensive area of the defect, forming a more prominent immature bone matrix near the edges of the defects, with the presence of graft particles dispersed in the central area. The presence of the type I collagen membrane was shown to be an important tool in the acceleration of bone remodeling used in all experimental groups, in which, in comparison to contralateral defects in which the membrane was not used, a significant increase of bone just graduated. At the systemic level, the results of ozone treatment allowed the reduction of adverse effects of glucocorticoids, such as the reduction of the body weight of the animals and atrophy of lymphatic organs such as spleen, observed in the groups treated alone with dexamethasone. Conclusion: Given the limitations of the animal study, the use of systemic ozone associated with the xenograft stimulates bone remodeling in critical defects of immunosuppressed rats. / Objetivo: O presente estudo teve por objetivo avaliar os efeitos da ozonioterapia sist?mica sobre o remodelamento ?sseo de defeitos cr?ticos na calota de ratos, com e sem a presen?a de xenoenxerto, utilizando um modelo animal de imunossupress?o induzido por corticoterapia. M?todos: Foram utilizados 64 ratos machos Wistar (180-220 g), distribu?dos em 8 grupos experimentais (N = 8/grupo). Para a corticoterapia (grupos II, IV, VI e VIII), os animais receberam dexametasona (1 mg/kg; i.p.), uma inje??o a cada 48 h, com in?cio duas semanas antes dos procedimentos cir?rgicos, se estendendo at? a eutan?sia. Os animais controles (grupos I, III, V e VII) receberam solu??o salina (10 ml/kg; i.p.), nos mesmos intervalos de tempo. Ap?s duas semanas do in?cio da corticoterapia, os animais foram anestesiados com quetamina e xilazina (100 e 10 mg/kg; i.p.), para a confec??o de dois defeitos cr?ticos nos ossos parietais da calota craniana (5 mm de di?metro). Os defeitos foram preenchidos com co?gulo (grupos I, II, V e VI) ou com xenoenxerto Bio-Oss? (Geistlish Biomaterials, Germany) (grupos III, IV, VII e VIII). Em todos os grupos, o defeito da direita foi coberto por uma membrana de col?geno Bio-Gide? (Geistlish Biomaterials, Germany). Para a ozonioterapia sist?mica, os animais dos grupos V, VI, VII e VIII receberam uma aplica??o de oz?nio (0,7 mg/kg; i.p.), todos os dias, durante 7 dias, iniciando imediatamente ap?s a cirurgia. Decorridos quatro semanas da cria??o dos defeitos, os animais foram eutanasiados e as calotas cranianas foram coletadas para avalia??o histol?gica da neoforma??o ?ssea, com colora??o de hematoxilina e eosina (HE). Os protocolos foram aprovados pela Comiss?o de ?tica no Uso de Animais (7691). Foram realizadas an?lises histol?gicas qualitativas baseado no aspecto do trabeculado ?sseo, atividade osteobl?stica em torno da matriz ?ssea, padr?o de forma??o de tecido conjuntivo na ?rea do defeito, e exist?ncia de part?culas de enxerto na regi?o. Resultados: Os grupos V, VII apresentaram maiores ?reas ?sseas ao longo da superf?cie do defeito, em compara??o com os VI e VIII, como consequ?ncia dos efeitos potenciais do oz?nio no remodelamento ?sseo, minimizando as interfer?ncias negativas na microarquitetura ?ssea causada pelos glicocortic?ides. A presen?a do xenoenxerto nos grupos III, IV, VII, VIII, proporcionou o recobrimento de extensa ?rea do defeito, formando matriz ?ssea imatura mais proeminente nas proximidades dos bordos dos defeitos, com presen?a de part?culas de enxerto dispersas na ?rea central. A presen?a da membrana de col?geno tipo I, se mostrou um importante recurso na acelera??o do remodelamento ?sseo utilizado em todos os grupos experimentais, na qual em compara??o com os defeitos contralaterais em que n?o foi utilizado a membrana, pode ser visto um significativo aumento de osso rec?m-formado. A n?vel sist?mico, os resultados do tratamento com oz?nio possibilitaram a diminui??o de efeitos adversos dos glicocorticoides, tais como a diminui??o do peso corporal dos animais e atrofia de ?rg?os linf?ticos como o ba?o, observados nos grupos tratados isoladamente com a dexametasona. Conclus?o: Diante das limita??es do estudo animal, a utiliza??o de oz?nio sist?mico associado ao xenoenxerto, estimula o remodelamento ?sseo em defeitos cr?ticos de ratos imunossuprimidos.

Submitted by Sandra Pereira (srpereira@ufrrj.br) on 2017-04-07T12:52:15Z No. of bitstreams: 1 2014 - Carlos Henrique Rocha Gon?alves.pdf: 2447515 bytes, checksum: bcb566dd037e0d15e3e3ab74112d585e (MD5) / Made available in DSpace on 2017-04-07T12:52:15Z (GMT). No. of bitstreams: 1 2014 - Carlos Henrique Rocha Gon?alves.pdf: 2447515 bytes, checksum: bcb566dd037e0d15e3e3ab74112d585e (MD5) Previous issue date: 2014-02-27 / Coordena??o de Aperfei?oamento de Pessoal de N?vel Superior - CAPES / This work aimed to realize oxidative reactions using ozone in aqueous middle to simulate the aging aspect in Corymbia citriodora K.D. Hill & L.A.S. Johnson wood samples. Ozone that is a very reactive gas, was produced by the ozonizer by the corona effect. In order to evaluate the transformations occurred in the samples submitted to the treatment in different times and pHs (neutral, basic and acid) those samples were analyzed by various tests (color mensuration in the CIELAB colorimetric space; contact angle between water and wood by the goniometer; XPS spectroscopy and Pyrolysis ? GC/MS). The samples were obtained from a Corymbia citriodora tree log harvested in the UFRRJ campus in Serop?dica. Those samples were treated mechanically in order to adequate it to the analysis with 10 x 20 x 2 mm dimensions. It was observed that the 6h acid was the one that presented the best natural aging appearance. All the tests done have shown that the wood components had different behaviors in each treatment, especially when we talk about lignin and its G/S (Guaiacyl:Siringyl). The results have shown that the reactions were well succeed, revealing that the ozone had reacted in the majority of the samples, with the lignin, affecting the G/S ratio for all the treatments. XPS data showed the level of oxidation, for example, in the neutral treatment, showing ozone oxidative effect. In this way, the G/S ratio revealed that the ozone attack have occurred, first with the lignins that have predominantly the guaiacyl units, assuming that the reaction affected mainly the cellular region with high guaiacyl units content. It was also detected in the acid treatment, that the ozone as well reached the extractives group, especially the decanoic acids. All the experimentations were sufficient to discolor and give aging appearance to the wood, as the color changing shown by the color tests with the spectrophotometer. The contact angle has proved that the samples in order absorb more water, becoming more hydrophilic, when related to the L:C ratio (lignin:carbohydrate). / Esse trabalho teve por objetivo realizar rea??es oxidativas utilizando oz?nio em meio aquoso para simular o aspecto de envelhecimento em madeiras de Corymbia citriodora K.D. Hill & L.A.S. Johnson. O oz?nio que ? um g?s extremamente reativo foi produzido pelo ozonizador atrav?s da descarga corona. Para avaliar as modifica??es ocorridas nas amostras ap?s serem submetidas ?s rea??es de ozon?lise em diferentes tempos e pHs (neutro, b?sico e ?cido), essas amostras foram posteriormente submetidas a v?rios testes (mensura??o da cor no espa?o colorim?trico CIELAB; do ?ngulo de contato entre a ?gua e a madeira atrav?s do goni?metro; espectroscopia XPS (Espectroscopia Fotoeletr?nica de Raios-X) e pir?lise ? CG/EM (pir?lise analisada por cromatografia gasosa, acoplada ? um detector de massa)). As amostras foram obtidas de tora de madeira de um esp?cime da esp?cie Corymbia citriodora obtida no campus da UFRRJ em Serop?dica. Essas amostras foram tratadas mecanicamente de maneira a adequ?-la para an?lise, ficando com dimens?o de 10 x 20 x 2 mm. Foi constatado que as amostras do tratamento ?cido durante 6h foram as que ficaram com a apar?ncia mais pr?xima de uma madeira naturalmente envelhecida. Os diversos testes feitos mostraram que os componentes da madeira se comportaram de forma diferente em cada um dos tratamentos especialmente no que tange ? lignina na sua raz?o G/S da mesma. Os resultados mostraram que a rea??es foram bem sucedidas, revelando que o oz?nio reagiu na grande maioria das amostras com a lignina afetando a raz?o G/S para todos os tratamentos. Dados do XPS mostram os n?veis de oxida??o por exemplo dos tratamentos neutros, mostrando o efeito oxidativo do oz?nio. Neste sentido, a raz?o Guacila/Siringila revelou que o ataque do oz?nio aconteceu primeiramente com as ligninas que predominam unidades guaiacila, pressupondo que a rea??o afetou direcionadamente a regi?o da parede celular com mais alto teor de unidade Guaiac?la. Foi tamb?m detectado no tratamento ?cido que o oz?nio atingiu tamb?m o grupo dos extrativos especialmente os ?cidos decan?icos. Todos os experimentos foram suficientes para descolorir e dar apar?ncia de madeira envelhecida, como mudan?as de cor mostradas pelos testes de cor com espectrofot?metro. O ?ngulo de contato mostrou que as amostras em ordem absorvem mais ?gua, tornando-as mais hidrof?lica, quando correlacionada ? raz?o C:L (Carboidratos:Lignina).

Submitted by Jorge Silva (jorgelmsilva@ufrrj.br) on 2018-03-28T19:08:17Z No. of bitstreams: 1 2017 - Yuri Duarte Porto.pdf: 1910618 bytes, checksum: 969c177c9241195f2d92cb156f231abb (MD5) / Made available in DSpace on 2018-03-28T19:08:17Z (GMT). No. of bitstreams: 1 2017 - Yuri Duarte Porto.pdf: 1910618 bytes, checksum: 969c177c9241195f2d92cb156f231abb (MD5) Previous issue date: 2017-04-06 / CAPES / Corn is one of the most cultivated cereals in Brazil and it is susceptible to contamination by mycotoxins that are toxic secondary metabolites produced by fungi. An adoption of quality management systems during a corn production chain is essential to ensure food safety concerning mycotoxin contamination. However, there is a possibility that these recognized carcinogenic metabolites may already be found in the matrix. Hence the search for alternative solutions capable of reducing contamination to safe levels by the application of emerging technologies has been intense. The methods of decontamination of foods must to obey some premises as: inactivate, destroy or remove the toxins; do not produce others toxic waste; maintain the nutritional value and acceptability of the product. Ozone, which meets almost all of these characteristics, is recognized by the Food and Drug Administration as a GRAS (Generally Recognized as Safe) product for use as a sanitizer in food processing. Since then its use has been investigated in several types of food, including cereals contaminated by mycotoxins. Corn canjiquinha is a cultural food easily accessible by Brazilian consumers at low prices, as well as posing a potential risk of exposure due to contamination with aflatoxins. In this study, aliquots of canjiquinha samples were inoculated with concentrations of 106 CFU/g conidia of Aspergillus spp. and Fusarium spp. previously isolated from this food. Other aliquots were contaminated with aflatoxins B1, B2, G1 and G2 at a concentration of 50 ?g/kg for each. The application of gaseous ozone was tested in different combinations of exposure time, ozone concentration and canjiquinha mass, being these independent variables investigated. After treatment by ozonation, to evaluate the effects on aflatoxin concentrations, the samples were analyzed by high-performance liquid chromatography with fluorescence detection (HPLC-FD system) with pre-column derivatization (C18) by trifluoroacetic acid (TFA). The analytical method used in this study was optimized and parameters such as accuracy, precision, linearity, selectivity, limits of detection and limits of quantification of the analytical method were previously evaluated. Additionally, aflatoxin quantification tests were carried out on commercial samples of corn kernels. The method was adequate and presented recovery values within the range of 80-110% with a coefficient of variation of less than 15%, with detection and quantification limits equal to 0.8 and 3.6 ?g/kg, respectively, for each of the aflatoxins. Isolation of fungi was carried out according to the criteria of the Brazilian Ministry of Agriculture. Thus, the efficacy of gaseous ozonation on aflatoxin levels B1, B2, G1 and G2 and on microbial contamination of canjiquinha grains showed reductions of up to 57% in aflatoxin levels. The total fungal count had a reduction of about 3.0 log cycles UFC/g and the total counts of mesophiles were reduced to undetectable levels. These results demonstrated that ozonation is an effective alternative to reduce microbial contamination and the concentration of aflatoxins in corn kernels and, consequently, can improves the safety of this product. / O milho ? um dos cereais mais cultivados no Brasil e est? exposto ? contamina??o por micotoxinas que s?o metab?litos t?xicos secund?rios produzidos por fungos. A ado??o de sistemas de gest?o da qualidade durante a cadeia de produ??o de milho ? essencial para garantir a seguran?a do alimento quanto ? contamina??o por micotoxinas. Contudo, h? possibilidade desses metab?licos reconhecidamente carcinog?nicos j? encontrarem-se na matriz. Da? a busca por solu??es alternativas capazes de reduzir a contamina??o para n?veis seguros pela aplica??o de tecnologias emergentes tem sido intensa. Os m?todos de descontamina??o de alimento devem obedecer algumas premissas como: inativar, destruir, ou remover as toxinas; n?o produzir res?duos t?xicos; manter o valor nutricional e a aceitabilidade do produto. O oz?nio atende quase todas estas caracter?sticas, ? reconhecido pelo United States Food and Drug Administration (FDA) como uma subst?ncia GRAS (Geralmente Reconhecido como Seguro) para uso como sanitizante no processamento de alimentos. Desde ent?o sua utiliza??o tem sido pesquisada em diversos tipos de alimentos, incluindo cereais contaminados por micotoxinas. A canjiquinha de milho ? um alimento cultural de f?cil aquisi??o pelo consumidor brasileiro pelo baixo pre?o, al?m de representar um potencial risco de exposi??o por contamina??o com aflatoxinas. Neste estudo, al?quotas de amostras de canjiquinha foram inoculadas concentra??es de 106 UFC/g de con?dios de Aspergillus spp. e Fusarium spp. previamente isolados do alimento. Outras al?quotas foram contaminadas com aflatoxinas B1, B2, G1 e G2 na concentra??o de 50 ?g/kg para cada. A aplica??o de oz?nio gasoso foi testada em diferentes combina??es de tempo de exposi??o, concentra??o do oz?nio e massa de canjiquinha, sendo essas as vari?veis independentes pesquisadas. Ap?s tratamento por ozoniza??o, para avalia??o dos efeitos sobre as concentra??es de aflatoxinas, as amostras foram analisadas por cromatografia l?quida de alta efici?ncia com detec??o por fluoresc?ncia (sistema CLAE-DF) com derivatiza??o pr?-coluna (C18) por ?cido trifluoroac?tico (TFA). O m?todo anal?tico utilizado neste estudo foi otimizado e par?metros como a exatid?o, precis?o, linearidade da faixa de trabalho, seletividade, limites de detec??o e limites de quantifica??o do m?todo anal?tico foram previamente avaliados. Adicionalmente efetuaram-se ensaios para quantifica??o das aflatoxinas em amostras comerciais de canjiquinha de milho. O m?todo mostrou-se adequado e apresentando valores de recupera??o dentro da faixa de 80-110% com coeficiente de varia??o menor que 15%, sendo os limites de detec??o e quantifica??o iguais a 0,8 e 3,6 ?g/Kg, respectivamente, para cada uma das aflatoxinas. O isolamento de fungos foi realizado de acordo com as normas do Minist?rio da Agricultura. Assim a avalia??o da efic?cia da ozoniza??o gasoso sobre os n?veis de aflatoxinas B1, B2, G1 e G2 e sobre a contamina??o microbiana dos gr?os de canjiquinha apresentou redu??es de at? 57% nos n?veis de aflatoxinas. A contagem de fungos totais teve uma redu??o de cerca de 3,0 ciclos log UFC/g e a contagem total de mes?filos foram reduzidas a n?veis n?o detect?veis. Estes resultados demonstraram que a ozoniza??o ? uma alternativa eficaz para reduzir a contamina??o microbiana e a concentra??o de aflatoxinas em canjiquinha de milho e, consequentemente, melhora a seguran?a desse produto.

Submitted by Rodrigo Martins Cruz (rodrigo.cruz@ufvjm.edu.br) on 2015-02-19T13:03:29Z No. of bitstreams: 5 lindomar.pdf: 4197857 bytes, checksum: 529e21e13238b7b71e5ddffb7f4e7b91 (MD5) license_url: 52 bytes, checksum: 3d480ae6c91e310daba2020f8787d6f9 (MD5) license_text: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5) license_rdf: 23898 bytes, checksum: e363e809996cf46ada20da1accfcd9c7 (MD5) license.txt: 2111 bytes, checksum: f5c843397da71f5d32b775fd8c4cbf56 (MD5) / Approved for entry into archive by Rodrigo Martins Cruz (rodrigo.cruz@ufvjm.edu.br) on 2015-02-20T09:29:04Z (GMT) No. of bitstreams: 5 lindomar.pdf: 4197857 bytes, checksum: 529e21e13238b7b71e5ddffb7f4e7b91 (MD5) license_url: 52 bytes, checksum: 3d480ae6c91e310daba2020f8787d6f9 (MD5) license_text: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5) license_rdf: 23898 bytes, checksum: e363e809996cf46ada20da1accfcd9c7 (MD5) license.txt: 2111 bytes, checksum: f5c843397da71f5d32b775fd8c4cbf56 (MD5) / Made available in DSpace on 2015-02-20T09:29:04Z (GMT). No. of bitstreams: 5 lindomar.pdf: 4197857 bytes, checksum: 529e21e13238b7b71e5ddffb7f4e7b91 (MD5) license_url: 52 bytes, checksum: 3d480ae6c91e310daba2020f8787d6f9 (MD5) license_text: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5) license_rdf: 23898 bytes, checksum: e363e809996cf46ada20da1accfcd9c7 (MD5) license.txt: 2111 bytes, checksum: f5c843397da71f5d32b775fd8c4cbf56 (MD5) Previous issue date: 2013-12-17 / Eletrodos constitu?dos de ?-PbO2 foram preparados via eletrodeposi??o, mediante inclus?o dos ?ons Fe3+ e F- na mistura precursora, usando como substratos tela de a?o (ASTM 316) e tecido de carbono, visando a produ??o eletroqu?mica de oz?nio (PEO) em um reator filtro-prensa conduzida na aus?ncia de eletr?lito l?quido. A an?lise das micrografias de MEV para ambos substratos revelaram que em baixas concentra??es dos dopantes houve a forma??o de gr?os bem definidos com uma ?rea superficial uniforme. A an?lise de Raios-X para ambos substratos revelou o desfavorecimento da forma??o da fase ?-PbO2 para a maioria da concentra??o dos dopantes. Estudos cronopotenciom?tricos evidenciaram a estabilidade dos anodos quando sujeitados a intensa evolu??o de gases. Para o eletrodo dopado com 1 mM do ?on Fe3+, a efici?ncia de corrente da PEO foram de 10% e 9,5% com uma velocidade de gera??o de oz?nio de 1,35 g h-1 e 1,40 g h-1 para os substratos de tela de a?o e de tecido de carbono, respectivamente. Os resultados encontrados s?o promissores indicando uma viabilidade do uso desses eletrodos para aplica??o no tratamento de ?gua, por isso, empregou-se o oz?nio produzido eletroquimicamente para tratar ?gua residu?ria proveniente da produ??o de biodiesel na planta piloto da UFVJM. O tratamento oxidativo da ?gua residu?ria foi conduzido num reator em coluna de bolhas via rea??o direta (oz?nio molecular), indireta (radical hidroxila) e mista (O3/HO?) manipulando-se o pH do meio reacional.A caracteriza??o do efluente ozonizado foi efetuada monitorando-se o processo de degrada??o da mat?ria org?nica dissolvida via espectrofotometria. A cin?tica do processo de degrada??o seguiu o modelo de pseudo-primeira ordem. Foi verificado que a constante cin?tica sofre varia??o com o tempo de ozoniza??o revelando, portanto, uma varia??o da recalcitr?ncia com o tempo de rea??o. A demanda de energia requerida no processo de ozoniza??o foi caracterizada por uma energia el?trica por ordem (EEO) de 139 kW h m-3 ordem-1. / Disserta??o (Mestrado) ? Programa de P?s-Gradua??o em Qu?mica, Universidade Federal dos Vales do Jequitinhonha e Mucuri, 2013. / ABSTRACT Electrodes comprised of ?-PbO2 were prepared by electrodeposition, by moans of the inclusion of Fe3+ and F- ions in the precursor mixture, using as substrates steel screen (ASTM 316) and carbon cloth in order to produce ozone in an filter-press electrochemical reactor conducted in the absence of liquid electrolyte. The analysis of SEM micrographs revealed that for both substrates at low dopants concentrations resulted in the formation of well-defined grains with a uniform surface area. X-rays analysis for both substrates showed the inhibition of formation of the ?-PbO2 phase for most of the dopant concentration. Cronopotenciometric studies revealed a good stability of the anodes when they were subjected to the intense gas evolution. For the electrode doped with 1 mM Fe3+, the current efficiency of EOP were 10% and 9.5% for an ozone generation rate of 1.35 g h-1 and 1.40 g h -1 using the steel screen and carbon cloth substrates, respectively. These results are promising, indicating a feasibility of using these electrodes for application in water purification, so the electrochemical ozone produced was applied to treat residuary water obtained from the biodiesel produced in the pilot plant of UFVJM. The oxidative chemical treatment of wastewater was conducted in a bubble column reactor via direct (molecular ozone), indirect (hydroxyl radical) and mixed (O3/HO?) oxidation reaction thrush manipulating the pH of the reaction medium. The characterization of ozonated effluent was assessed by means of monitoring the degradation of the dissolved organic matter using spectrophotometry. The kinetics of the degradation process followed a pseudo-first order model. It was found that the rate constant changes on the ozonation time thus indicating a recalcitrance variation with the reaction time. The energy demand required in the ozonation process was described by an electric energy per order (EEO) of 139 kW h m-3 order-1.

Submitted by Sandra Pereira (srpereira@ufrrj.br) on 2017-01-04T11:32:19Z No. of bitstreams: 1 2016 - Felipe Machado Trombete.pdf: 1613295 bytes, checksum: 957f0b025def400e446f7c3176c76b4c (MD5) / Made available in DSpace on 2017-01-04T11:32:19Z (GMT). No. of bitstreams: 1 2016 - Felipe Machado Trombete.pdf: 1613295 bytes, checksum: 957f0b025def400e446f7c3176c76b4c (MD5) Previous issue date: 2016-03-18 / Coordena??o de Aperfei?oamento de Pessoal de N?vel Superior - CAPES / Mycotoxins are natural contaminants produced by filamentous fungi and they are widely present in food. In wheat grains, the presence of trichothecenes produced by Fusarium spp. and also aflatoxins, produced by Aspergillus spp. are a serious public health problem because they are toxic metabolites to man and animals that remain stable during the industrial processes to which wheat is subjected when manufacturing derived products. One way to reduce such contaminants is to use ozone (O3) in food processing. Due to its high potential as an oxidant, O3 may react with mycotoxins and reduce their toxicity due to molecular degradation. This research aimed to evaluate the effects of different conditions of ozonation on the i) reduction in mycotoxins levels (deoxynivalenol and total aflatoxins); ii): reduction in total fungal count; iii) the effects on chemical, mineral and technological profiles and; iv) the possible differences sensorial profile of the flour obtained from the ozonized grains. In a second phase of this project, a methodology for determination of D3G (deoxynivalenol-3-glucoside), a masked form of DON, using High Performance Liquid Chromatography with photodiode array detector (HPLC-PDA) was optimized and in-house validated. Results obtained from ozonation study showed that O3 reduced total fungal count in approximately 3.0 cycles log CFU/g of wheat grain and deoxynivalenol and total aflatoxins contamination up to 64.3% and 48.0%, respectively. The gaseous ozonation can be applied without negatively changing the chemical, technological and sensory characteristics of the grains and can be considered an excellent method for remediation of fungal and mycotoxin contaminations. Also, the method optimized and in-house validated for determination of D3G by HPLC-PDA showed adequate results and, it could be considered an alternative to mass spectrometry determination of D3G in wheat grains. / Micotoxinas s?o contaminantes naturais, produzidos por fungos filamentosos e, podem ocorrer em altos n?veis nos alimentos. Nos gr?os de trigo, a presen?a de tricotecenos, um grupo de micotoxinas produzidas por Fusarium spp. e, aflatoxinas, produzidas por Aspergillus spp., representam um importante problema de sa?de p?blica por serem t?xicas ao homem e animais e muito est?veis aos processos no qual o trigo ? submetido para obten??o de produtos industrializados. Uma forma de reduzir a contamina??o dos alimentos por micotoxinas ? atrav?s do uso do oz?nio (O3) no processamento do alimento. Devido ao alto potencial oxidante do O3, esse pode degradar as mol?culas das micotoxinas, tendo como consequ?ncia a elimina??o ou redu??o de seus efeitos t?xicos. Essa pesquisa teve como objetivos principais avaliar os efeitos de diferentes condi??es de ozoniza??o na i) redu??o de micotoxinas (desoxinivalenol e aflatoxinas) em gr?os de trigo; ii) redu??o nos n?veis de fungos filamentosos; iii) influ?ncia nos par?metros qu?micos, perfil de minerais e par?metros tecnol?gico dos gr?os e da farinha obtida ap?s o processamento e; iv) influ?ncia nas caracter?sticas sensoriais da farinha elaborada a partir dos gr?os ozonizados. Em uma segunda etapa do projeto, um m?todo para determina??o de uma forma modificada do desoxinivalenol (DON), o desoxinivalenol-3-glicos?deo (D3G) foi otimizada e validada intralaboratorialmente, utilizando cromatografia l?quida de alta efici?ncia com detector de arranjo de diodos (CLAE-DAD). Os resultados obtidos dos ensaios de ozoniza??o demonstraram que o O3, nas condi??es experimentais utilizadas, reduziu a contagem de fungos totais em cerca 3,0 logs UFC/g de gr?os e a contamina??o por desoxinivalenol e aflatoxinas totais em at? 64,3 % e 48,0 %, respectivamente. O processo de ozoniza??o n?o influenciou de modo negativo a qualidade qu?mica, tecnol?gica e sensorial dos gr?os de trigo, podendo ser utilizado como um excelente m?todo para remedia??o da contamina??o dos gr?os por fungos e micotoxinas. Resultados adequados tamb?m foram obtidos na valida??o do m?todo de determina??o de D3G por CLAE-DAD, demonstrando que o m?todo ? confi?vel para a determina??o dessa forma mascarada do DON em gr?os e trigo e, pode ser utilizado como um m?todo alternativo a espectrometria de massas para tal an?lise.

Made available in DSpace on 2014-12-17T14:12:03Z (GMT). No. of bitstreams: 1 AlexandreBL_TESE.pdf: 4327655 bytes, checksum: 57b0b9f8e05c550b153e77bf0aa25eff (MD5) Previous issue date: 2014-03-26 / Conselho Nacional de Desenvolvimento Cient?fico e Tecnol?gico / The increase in ultraviolet radiation (UV) at surface, the high incidence of non-melanoma skin cancer (NMSC) in coast of Northeast of Brazil (NEB) and reduction of total ozone were the motivation for the present study. The overall objective was to identify and understand the variability of UV or Index Ultraviolet Radiation (UV Index) in the capitals of the east coast of the NEB and adjust stochastic models to time series of UV index aiming make predictions (interpolations) and forecasts / projections (extrapolations) followed by trend analysis. The methodology consisted of applying multivariate analysis (principal component analysis and cluster analysis), Predictive Mean Matching method for filling gaps in the data, autoregressive distributed lag (ADL) and Mann-Kendal. The modeling via the ADL consisted of parameter estimation, diagnostics, residuals analysis and evaluation of the quality of the predictions and forecasts via mean squared error and Pearson correlation coefficient. The research results indicated that the annual variability of UV in the capital of Rio Grande do Norte (Natal) has a feature in the months of September and October that consisting of a stabilization / reduction of UV index because of the greater annual concentration total ozone. The increased amount of aerosol during this period contributes in lesser intensity for this event. The increased amount of aerosol during this period contributes in lesser intensity for this event. The application of cluster analysis on the east coast of the NEB showed that this event also occurs in the capitals of Paraiba (Jo?o Pessoa) and Pernambuco (Recife). Extreme events of UV in NEB were analyzed from the city of Natal and were associated with absence of cloud cover and levels below the annual average of total ozone and did not occurring in the entire region because of the uneven spatial distribution of these variables. The ADL (4, 1) model, adjusted with data of the UV index and total ozone to period 2001-2012 made a the projection / extrapolation for the next 30 years (2013-2043) indicating in end of that period an increase to the UV index of one unit (approximately), case total ozone maintain the downward trend observed in study period / A eleva??o da radia??o ultravioleta (UV), a alta incid?ncia de c?ncer de pele n?o melanoma (CPNM) no Nordeste do Brasil (NEB) e a redu??o da coluna total de oz?nio foram os motivadores do presente estudo. O objetivo desta investiga??o ? identificar e compreender a variabilidade da UV e do ?ndice de Radia??o Ultravioleta (?ndice UV) nas capitais da costa leste do NEB e ajustar modelos estoc?sticos ?s s?ries temporais do ?ndice UV visando realizar predi??es (interpola??es) e previs?es/proje??es (extrapola??es) seguido de an?lise de tend?ncia. A metodologia consistiu da aplica??o da an?lise multivariada (an?lise de componentes principais e an?lise de agrupamentos), m?todo Predictive Mean Matching (PMM) para preenchimento de falhas nos dados, modelo autoregressivo de defasagens distribu?das ou Autoregressive Distributed Lag (ADL) e teste Mann-Kendal. A modelagem via ADL ocorreu atrav?s da estima??o de par?metros, diagn?stico, an?lise de res?duos e avalia??o da qualidade das predi??es/previs?es via erro quadr?tico m?dio e coeficiente de correla??o de Pearson. Os resultados da investiga??o indicaram que a variabilidade anual da UV na capital do Rio Grande do Norte (Natal) possui uma caracter?stica nos meses de setembro e outubro que consiste em uma estabiliza??o/redu??o do ?ndice UV em raz?o da maior concentra??o anual de oz?nio total. A maior quantidade de aeross?is neste per?odo contribui em menor intensidade para este evento. A aplica??o da an?lise de agrupamento (cluster) na costa leste do NEB mostrou que este evento tamb?m ocorre nas capitais da Para?ba (Jo?o Pessoa) e Pernambuco (Recife). Os eventos extremos da UV do NEB foram analisados a partir da cidade de Natal e estavam associados ? falta de cobertura de nuvens e n?veis abaixo da m?dia anual de oz?nio total e n?o ocorriam na totalidade da regi?o em fun??o da distribui??o espacial desigual dessas vari?veis. O modelo ADL (4, 1), ajustado com dados do ?ndice UV e oz?nio total para o per?odo de 2001 a 2012, realizou a proje??o/extrapola??o para os pr?ximos 30 anos (2013-2043) indicando ao fim deste per?odo eleva??o de aproximadamente uma unidade do ?ndice UV, caso o oz?nio total mantenha a tend?ncia de queda verificada no per?odo de estudo

Made available in DSpace on 2014-12-17T14:58:09Z (GMT). No. of bitstreams: 1 JoaoCBM_DISSERT.pdf: 5805236 bytes, checksum: 65458c7bbde564338d0a049065004604 (MD5) Previous issue date: 2010-12-20 / From what was stated in the Montreal Protocol, the researchers and refrigeration industry seek substitutes for synthetic refrigerants -chlorofluorocarbons (CFCs) and HCFCs (HCFC) - that contribute to the depletion of the ozone layer. The phase-out of these substances was started using as one of the replacement alternatives the synthetic fluids based on hydro fluorocarbons (HFCs) that have zero potential depletion of the ozone layer. However, contribute to the process of global warming. HFC refrigerants are greenhouse gases and are part of the group of gases whose emissions must be reduced as the Kyoto Protocol says. The hydrocarbons (HC's), for not contribute to the depletion of the ozone layer, because they have very low global warming potential, and are found abundantly in nature, has been presented as an alternative, and therefore, are being used in new home refrigeration equipment in several countries. In Brazil, due to incipient production of domestic refrigerators using HC's, the transition refrigerants remain on the scene for some years. This dissertation deals with an experimental evaluation of the conduct of a drinking fountain designed to work with HFC (R-134a), operating with a mixture of HC's or isobutane (R-600a) without any modification to the system or the lubricating oil. In the refrigeration laboratory of Federal University of Rio Grande do Norte were installed, in a drinking fountain, temperature and pressure sensors at strategic points in the refrigeration cycle, connected to an acquisition system of computerized data, to enable the mapping and thermodynamics analysis of the device operating with R-134a or with a mixture of HC's or with R-600a. The refrigerator-test operating with the natural fluids (mixture of HC's or R-600a) had a coefficient of performance (COP) lower than the R-134a / A partir do estabelecido no Protocolo de Montreal, os pesquisadores e a ind?stria de refrigera??o buscam substitutos para os refrigerantes sint?ticos clorofluorcarbonos (CFC s) e os hidroclorofluorcabonos (HCFC s), que contribuem para a degrada??o da camada de oz?nio. O phase- out dessas subst?ncias foi iniciado utilizando como uma das alternativas de substitui??o os fluidos sint?ticos baseados em hidrofluorcarbonos (HFC s) que possuem potencial nulo de deple??o da camada de oz?nio, entretanto, contribuem para o processo de aquecimento global. Os refrigerantes HCF s s?o gases de efeito estufa e fazem parte do grupo de gases cujas emiss?es devem ser reduzidas, conforme Protocolo de Kyoto. Os hidrocarbonetos (HC s), por n?o contribu?rem para a degrada??o da camada de oz?nio, por terem muito baixo potencial de aquecimento global, e por serem encontrados abundantemente na natureza, t?m sido apresentados como alternativa e, por isso, est?o sendo usados em novos equipamentos de refrigera??o dom?stica em diversos pa?ses. No Brasil, devido ? produ??o incipiente de refrigeradores dom?sticos utilizando HC s, os refrigerantes de transi??o continuar?o em cena por mais alguns anos. Este trabalho trata de uma avalia??o experimental do comportamento de um bebedouro projetado para trabalhar com HFC (R-134a), operando com uma mistura de HC s ou isobutano (R-600a) sem nenhuma modifica??o no sistema ou no ?leo lubrificante. No laborat?rio de refrigera??o da Universidade Federal do Rio Grande do Norte foram instalados, em um bebedouro, sensores de press?o e temperatura em pontos estrat?gicos do ciclo de refrigera??o, conectados a um sistema de aquisi??o de dados computadorizado, de modo a permitir o mapeamento e a an?lise termodin?mica do dispositivo operando com R-134a ou com a mistura HC s ou com R-600a. O refrigerador de testes, operando com fluidos naturais (mistura de HC s ou R-600a), apresentam um coeficiente de performance (COP) inferior ao do R-134a

"Resumo/Abstract", "palavras-chave/keywords" nos cap?tulos trabalho. / Submitted by Jos? Henrique Henrique (jose.neves@ufvjm.edu.br) on 2017-03-27T20:52:05Z No. of bitstreams: 2 license_rdf: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5) fernanda_gandra_oliveira.pdf: 3187185 bytes, checksum: 690bd6e7967bbab89412d221de93c88a (MD5) / Approved for entry into archive by Rodrigo Martins Cruz (rodrigo.cruz@ufvjm.edu.br) on 2017-04-24T14:26:09Z (GMT) No. of bitstreams: 2 license_rdf: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5) fernanda_gandra_oliveira.pdf: 3187185 bytes, checksum: 690bd6e7967bbab89412d221de93c88a (MD5) / Made available in DSpace on 2017-04-24T14:26:09Z (GMT). No. of bitstreams: 2 license_rdf: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5) fernanda_gandra_oliveira.pdf: 3187185 bytes, checksum: 690bd6e7967bbab89412d221de93c88a (MD5) Previous issue date: 2016 / Coordena??o de Aperfei?oamento de Pessoal de N?vel Superior (CAPES) / Funda??o de Amparo ? Pesquisa do Estado de Minas Gerais (FAPEMIG) / O comp?sito nFZV-CAG foi empregado para a ozoniza??o catal?tica heterog?nea da NMS em meio aquoso. O oz?nio foi gerado por uma central geradora de oz?nio IPABRAS, alimentada com ar. Foram avaliados diferentes processos catal?ticos como O3, CAG, O3-CAG, O3-nFZV- CAG, e a varia??o da concentra??o das nFZV para remo??o da NMS. Os resultados mostraram que a combina??o do O3-nFZV-CAG foi muito eficiente levando ? mineraliza??o de aproximadamente 70% da NMS em 120 min de rea??o. Tal efici?ncia pode estar atribu?da ao processo de eletr?lise, em que o Fe0 origina Fe2+ levando ? produ??o do radical hidroxila que ? altamente oxidante, levando a destrui??o do contaminante. As rea??es seguiram a cin?tica pseudo-primeira ordem para remo??o do f?rmaco. Ap?s realizada a coleta as amostras foram submetidas a an?lise qu?mica, empregando-se as t?cnicas de espectrofotometria de UV-Vis, cromatografia l?quida de alta efici?ncia (CLAE) e a determina??o da demanda qu?mica de oxig?nio (DQO) tamb?m foi realizada. / As nanopart?culas de FZV imobilizadas sobre a superf?cie do carv?o ativado (nFZV-CAG) foram sintetizadas e caracterizadas para remo??o do f?rmaco Nimesulida (NMS) em sistemas aquosos. Os estudos foram realizados em bateladas com agita??o de 250 rpm durante 120 minutos, onde foram avaliadas a efici?ncia, concentra??o (20, 25 e 30% do comp?sito) e diferentes dosagens (0,1 a 10g) de nFZV-CAG. Os resultados mostraram que a dosagem de 10 g de 20%nFZV-CAG removeu cerca de 80% da NMS 50 mg L-1 em apenas 30 minutos de rea??o, e atingindo 100% em 120 minutos. Foi observada ainda uma remo??o de 80% da DQO ao final da rea??o. As velocidades de rea??o aumentaram na medida em que foram aumentadas as dosagens do comp?sito, o que seria esperado. Por?m, com o aumento da concentra??o (%) de nFZV n?o houve aumento na velocidade das rea??es. As rea??es seguiram uma cin?tica de pseudo-primeira ordem em rela??o ? remo??o da NMS. Ap?s realizada a coleta, as amostras foram submetidas a an?lise qu?mica, empregando-se as t?cnicas de espectrofotometria de UV- VIS, cromatografia l?quida de alta efici?ncia (CLAE) e a determina??o da demanda qu?mica de oxig?nio (DQO) tamb?m foi realizada. Para caracteriza??o do comp?sito foram empregadas as t?cnicas Microscopia Eletr?nica de Varredura acoplada ? Espectrometria de Energia Dispersiva de Raios-X (MEV-EDS), que mostraram claramente a presen?a da nanopart?culas sobre a superf?cie do carv?o, e analise de superf?cie do nFZV-CAG e do CAG tamb?m foram realizadas. / Disserta??o (Mestrado) ? Programa de P?s-Gradua??o em Qu?mica, Universidade Federal dos Vales do Jequitinhonha e Mucuri, 2016. / The FZV nanoparticles immobilized onto the surface of the activated charcoal (nFZV-CAG) were synthesized and characterized for the removal of the pharmaceutical Nimesulide (NMS) in aqueous systems. The studies were performed in batch mode with stirring speeds of 250 rpm during 120 minutes, so that it was possible to evaluate efficiency, concentration (20, 25 and 30% of the composite) and different dosages (0,1 to 10g) of nFZV-CAG. The results showed that the dosage of 10g of 20%nFZV-CAG removed approximately 80% of NMS 50 mg L-1 in just 30 minutes of reaction, and reaching 100% in 120 minutes. It was still observed a removal of 80% of the COD (Chemical Oxygen Demand) at the end of the reaction. The reaction rates increased with the dosage of the composite, which was expected. Though, increasing the concentration (%) of nFZV did not result in higher reaction rates. The reactions followed a pseudo-first order kinetics for the removal of NMS. After the samples were collected, they were submitted to chemical analysis, employing the techniques of UV-VIS spectroscopy, high performance liquid chromatography (HPLC) and chemical oxygen demand (COD). In order to characterize the composite, the following techniques were used: Scanning Electron Microscopy (SEM) coupled with Energy Dispersive x-Ray spectroscopy (EDS), which showed clearly the presence of nanoparticles onto the charcoal surface. Analysis of the surface of nFZV-CAG were also performed. / The composite nFZV-CAG was used for the catalytic ozonation of NMS in aqueous media. The ozone was generated by an ozone generator IPABRAS, fuelled with air. Different catalytic processes were evaluated such as O3, CAG, O3-CAG, O3-nFZV-CAG, and the variation of the nFZV concentration for the removal of NMS. The results showed that the combination of O3- nFZV-CAG was very efficient, leading to the mineralization of approximately 70% of NMS in 120 minutes of reaction. Such efficiency can be attributed to the electrolysis process, in which the Fe0 generates Fe2+ which generates hydroxyl radicals that are highly oxidant, leading to the destruction of the contaminant. The reactions followed the pseudo-first order kinetics for the removal of the pharmaceutical. After the samples were collected, they were submitted to chemical analysis such as, UV-VIS spectroscopy, high performance liquid chromatography (HPLC) and chemical oxygen demand (COD).