This diploma thesis deals with design and assessment of selected parts of the reinforced concrete frame of multi-storey administrative building according to the ultimate and serviceability limit state. The aim of this thesis was to design and assess the reinforced concrete structure of a slab above the first floor, one column, a foundation pad and a staircase. In addition to the static calculation, drawing documentation is included. Internal forces were obtained from the 3D model created in computational software Scia Engineer 16.1.

The aim of the final thesis is to design and structural design report of selected elements of prefabricated storage hall. Processing of foundation drawing, element composition and for each designed element formwork drawing and reinforcement. It is a single-storey hall, where the main structural system consists of a transversal frame columns and prestressed girder. In this final thesis I designed and structural assessed prestressed girder, reinforced concrete purlin, roof bracing, column, grade beam and foundation pad. The calculation software Scia Engineer is used to calculate the effects of internal forces. The results are compared using a simplified method. The design and structural assessment are made according to valide standards. The designed construction complies with the limit state of ultimate and serviceability.

This diploma thesis is being solved for documentation of building in the stage of execution. The building is designed as a showroom with car repair shop. The building site is located in the land registry of Ostrava – Vítkovice. Project documentation is designed accordingly to the valid Czech Republic’s laws, bills and building standards. The building is divided into two objects the first one is showroom with administration and the second one is the car repair shop with washing room and restrooms. These two objects are structurally and operationally connected. The largest plan dimensions are 68,56 x 38,36 m. The building is designed as a reinforced concrete frame in the administrative part and a reinforced concrete wall system in the car repair part. The roof is in both cases realized by using steel trusses. The reinforced concrete frame is lined with aerated concrete masonry. In the roof part it is cladded with sandwich steel panels. The building is founded on reinforced concrete foundation pads with combination of strip foundations. The car repair shop is designed as one floor building, whereas the administrative part is designed as two floor.

The diploma thesis is focused on the design and review of selected load-bearing elements of the precast hall. Girder is dimensioned in two variants: reinforced and prestressed concrete. Design and assessment of prestressed girder was carried out using the simplified method and the IDEA statica. Column and footing was also dimensioned. All calculations are done in accordance with Eurocode 2.

The diploma thesis is aimed for design and assessment main load bearing elements of a apart-ment building on the ultimate and serviceability limit state. Assessed parts of construction are reinforced concrete slab over the second floor, column and shear wall in the first floor, stairway slab and foundation pad. The elements are assessed in a structural design report according valid standard. There is created drawings. Internal forces are calculated using software Scia Engineer, where is modeled and loaded the construction.

The subject of this thesis is to create a project documentation of the design of new construction of fire station in Šumperk. The fire station is designed according to ČSN 73 5710 as a C type station that will be used by professional fire brigade of Olomouc district. The object is structurally divided into two parts - a masonry building, and a reinforced concrete frame. The administrative part of the building together with the base of the fire brigade, technical room, and a fire tower are designed as a masonry building made of Ytong cellular concrete units. One of the parts is designed as a two-level object, while the technical room and the fire tower are designed as a sigle-level object. The circumferential masonry is insulated by contact thermal insulation system. The floor construction is made of Spiroll prestressed floor slabs. The roof is designed as a warm flat roof. The garage is a single-level building with built-in structure of storerooms in part of the garage. The precast reinforced concrete frame is set on foundation pads and foundation sills. The horizontal and vertical supporting structure consists of reinforced concrete parts – columns, Spiroll floor slabs, floor girders, sway frames, purlins and small purlins. The whole object is covered by a warm flat roof. The external cladding consists of PUR isolating sanwich panels.

Die vorliegende Arbeit beschäftigt sich mit der mechanischen Untersuchung des menschlichen Fersenfettpolsters (FP) in Abhängigkeit von Alter und Geschlecht. Das menschliche FP stellt evolutionsgeschichtlich eine Anpassung an den aufrechten Gang dar. Durch Aufrichtung des Oberkörpers im Zweibeingang kam es zur Ganglinienverlängerung in Richtung Ferse und folglich zu einer Mehrbelastung des Rückfußes. Sie prägten die Funktion des FP, die Kräfte beim Aufsetzen der Ferse zu reduzieren. Das FP, das ein spezielles Unterhautfettgewebe ist und sich aus straffem und lockerem Bindegewebe zusammensetzt, kann die Kräfte durch Verteilen und Absorbieren vermindern. Bekannt ist, dass sich das mechanische Verhalten der Haut zwischen Mann und Frau unterscheidet. Da das FP ein Bestandteil der Haut ist, stellt sich als ein Schwerpunkt dieser Arbeit die Frage: Unterscheidet sich das FP mechanisch zwischen Mann und Frau? Aus naturwissenschaftlicher Sicht ist Altern ein natürlicher Mechanismus, der die Lebenskraft des Organismus durch Zellalterung und –tod reduziert. Aus evolutionärer Sicht wurde zugelassen, dass sich bestimmte Zellen bzw. Gewebe, welche hohen endogenen und exogenen Faktoren ausgesetzt sind, vollständig aber begrenzt regenerieren dürfen. Es wird als primäres Altern gekennzeichnet. Das primäre Altern kann positiv oder negativ durch äußere Einwirkungen auf den Organismus („Sekundäres Altern“) beeinflusst werden. Bindegewebe, welches hohen exogenen Faktoren ausgesetzt ist, sind insbesondere Schnittstellen zwischen „Biologischem System“ und „Umwelt“ (z.B. beim Menschen das Gewebe der Hautinnenfläche oder der Fußsohle). Es wird erwartet, dass das FP dem Alterungsprozess stark unterworfen ist, da es alltäglich mechanisch beansprucht wird. Folglich kann es zu einem mechanischen Funktionsverlust des FP kommen, das sich negativ auf die Belastbarkeit des Rückfußes auswirken kann. Die Entwicklung von altersbedingten Verschleißerkrankungen kann nicht ausgeschlossen werden. Als ein zweiter Schwerpunkt dieser Arbeit stellt sich die Frage: Unterscheidet sich das FP mechanisch zwischen Jung und Alt? Es gibt hinsichtlich der Thematik dieser Dissertation wenige Untersuchungen. Die Autoren kommen zum Teil zu unterschiedlichen Ergebnissen und Schlussfolgerungen, dass möglicherweise auf die unterschiedlich verwendete Methodik zurückzuführen ist. Die Vor- und Nachteile der bisher durchgeführten Experimente machen es schwierig, Stellungnahme zu beziehen, welche der Tests die zuverlässigsten Ergebnisse liefern. Seit den letzten 10-15 Jahren wurde immer häufiger Ultraschall als zusätzliche Informationsquelle in mechanischen Messplätzen integriert, um innere Kenndaten zum mechanischen Verhalten des FP abzuleiten. Allerdings waren die quasi-statischen Messungen und die geringen Kontaktkräfte der limitierende Faktor um das mechanische Verhalten valide zu charakterisieren. Mit einem eigens entwickelten Messplatz sollte dieser methodische Ansatz überholt werden. Der instrumentierte Belastungsschlitten ermöglicht die Aufnahme von dynamischen Ultraschallbildsequenzen unter mindestens 10-fach höheren Kontaktkräften bei fast doppelter Fersenkontaktgeschwindigkeit gegenüber den bisher bekannten Ultraschallexperimenten in der Literatur. Mögliche geschlechts- und altersspezifische Unterschiede im mechanischen Verhalten des FP sind grundlegend für die Orthopädie-Technik, die klinische Forschung und die Biogerontologie. Die Orthopädie-Technik benötigt insbesondere die Erkenntnisse zum mechanischen Verhalten der Haut an unterschiedlichen Stellen der unteren Extremität in Abhängigkeit von Alter und Geschlecht, um den Tragekomfort und die Bewegungseffizienz von Prothesen und Orthesen zu bessern. In der klinischen Forschung zeigt sich das Interesse an den altersspezifischen mechanischen Kenndaten, um im Zusammenhang zu klinischen Parametern die Entwicklung von orthopädischen Erkrankungen zu erforschen. Für die Biogerontologie wäre diese Art von Forschung relevant, um Zusammenhänge zu histologischen Parametern zu überprüfen, die direkt am Alterungsprozess des Bindegewebes beteiligt sind. Sie könnten zur Entschlüsselung des Mechanismus „Altern“ beitragen.

<p>Protein malfunction can cause human diseases, which makes the protein a target in the process of drug discovery. In-depth knowledge of how protein functions can widely contribute to the understanding of the mechanism of these diseases. Protein functions are determined by protein structures and their dynamic properties. Protein dynamics refers to the constant physical movement of atoms in a protein, which may result in the transition between different conformational states of the protein. These conformational transitions are critically important for the proteins to function. Understanding protein dynamics can help to understand and interfere with the conformational states and transitions, and thus with the function of the protein. If we can understand the mechanism of conformational transition of protein, we can design molecules to regulate this process and regulate the protein functions for new drug discovery. Protein Dynamics can be simulated by Molecular Dynamics (MD) Simulations.</p> <p>The MD simulation data generated are spatial-temporal and therefore very high dimensional. To analyze the data, distinguishing various atomic interactions within a protein by interpreting their 3D coordinate values plays a significant role. Since the data is humongous, the essential step is to find ways to interpret the data by generating more efficient algorithms to reduce the dimensionality and developing user-friendly visualization tools to find patterns and trends, which are not usually attainable by traditional methods of data process. The typical allosteric long-range nature of the interactions that lead to large conformational transition, pin-pointing the underlying forces and pathways responsible for the global conformational transition at atomic level is very challenging. To address the problems, Various analytical techniques are performed on the simulation data to better understand the mechanism of protein dynamics at atomic level by developing a new program called Probing Long-distance interactions by Tapping into Paired-Distances (PLITIP), which contains a set of new tools based on analysis of paired distances to remove the interference of the translation and rotation of the protein itself and therefore can capture the absolute changes within the protein.</p> <p>Firstly, we developed a tool called Decomposition of Paired Distances (DPD). This tool generates a distance matrix of all paired residues from our simulation data. This paired distance matrix therefore is not subjected to the interference of the translation or rotation of the protein and can capture the absolute changes within the protein. This matrix is then decomposed by DPD</p> <p>using Principal Component Analysis (PCA) to reduce dimensionality and to capture the largest structural variation. To showcase how DPD works, two protein systems, HIV-1 protease and 14-3-3 σ, that both have tremendous structural changes and conformational transitions as displayed by their MD simulation trajectories. The largest structural variation and conformational transition were captured by the first principal component in both cases. In addition, structural clustering and ranking of representative frames by their PC1 values revealed the long-distance nature of the conformational transition and locked the key candidate regions that might be responsible for the large conformational transitions.</p> <p>Secondly, to facilitate further analysis of identification of the long-distance path, a tool called Pearson Coefficient Spiral (PCP) that generates and visualizes Pearson Coefficient to measure the linear correlation between any two sets of residue pairs is developed. PCP allows users to fix one residue pair and examine the correlation of its change with other residue pairs.</p> <p>Thirdly, a set of visualization tools that generate paired atomic distances for the shortlisted candidate residue and captured significant interactions among them were developed. The first tool is the Residue Interaction Network Graph for Paired Atomic Distances (NG-PAD), which not only generates paired atomic distances for the shortlisted candidate residues, but also display significant interactions by a Network Graph for convenient visualization. Second, the Chord Diagram for Interaction Mapping (CD-IP) was developed to map the interactions to protein secondary structural elements and to further narrow down important interactions. Third, a Distance Plotting for Direct Comparison (DP-DC), which plots any two paired distances at user’s choice, either at residue or atomic level, to facilitate identification of similar or opposite pattern change of distances along the simulation time. All the above tools of PLITIP enabled us to identify critical residues contributing to the large conformational transitions in both HIV-1 protease and 14-3-3σ proteins.</p> <p>Beside the above major project, a side project of developing tools to study protein pseudo-symmetry is also reported. It has been proposed that symmetry provides protein stability, opportunities for allosteric regulation, and even functionality. This tool helps us to answer the questions of why there is a deviation from perfect symmetry in protein and how to quantify it.</p>

Um den Einfluss des Sitzpolsters in Radhosen auf den mechanischen Komfort besser zu verstehen, wurden der Satteldruck und die Beckenbewegung in Abhängigkeit der Belastungsintensität sowie -dauer beim Radfahren analysiert. Die Ergebnisse der initialen Datenanalyse deuten darauf hin, dass weniger die Stabilitätsparameter, sondern eher die Druckwahrnehmung für die Beurteilung des mechanischen Komforts maßgeblich ist. / In order to better understand the influence of the seat pad in cycling shorts on mechanical comfort, saddle pressure and pelvic movement were analyzed as a function of load intensity as well as duration during cycling. The results of the initial data analysis indicate that it is not so much the stability parameters but rather the pressure perception that is decisive for the assessment of mechanical comfort.

The Master’s thesis is focused on the analysis and design of selected members of load-bearing structure of an administration building according to the ultimate limit states (ULS) and seviceability limit states (SLS). The calculation and the analysis was supported by design software SCIA ENGINEER 2012. Structural analysis deals with the design of the reinforced concrete (RC) flat slab above the 4rd strorey which is particularly supported by RC columns and particularly lies on RC walls. Furthermore, the Master’s thesis contains analysis of some selected columns of last three storeys, column of lowest storey, construction of stairway between 4rd and 5th storeys. The work beside this deals with the calculation and design of foundation of the object. The rest parts of the load-bearing structure are not solved in the Master’s thesis.