A 59-year-old man with lung cancer, peripheral blood leukocytosis and thrombocytosis without peripheral lymphadenopathy and hepatosplenomegaly was found to have pancreatic extramedullary hematopoiesis (EMH) in association with an 'atypical' myeloproliferative disorder. Studies for the Philadelphia chromosome and bcr-abl fusion product were negative. This is the first documented case in the literature of isolated EMH in the pancreas.

No description available.

Lai Kit Ching Jan. / Thesis (M.Phil.)--Chinese University of Hong Kong, 2004. / Includes bibliographical references (leaves 121-141). / Abstracts in English and Chinese. / Abstract --- p.i / 摘要 --- p.iv / Acknowledgements --- p.vi / List of abbreviations --- p.vii / List of figures --- p.ix / Table of contents --- p.xi / Chapter Chapter 1 --- Introduction --- p.1 / Chapter 1.1 --- "The structure, function and regulation of growth hormone" --- p.1 / Chapter 1.2 --- Historical perspective of growth hormone secretagogues --- p.5 / Chapter 1.3 --- Ghrelin and growth hormone secretagogue receptor --- p.7 / Chapter 1.4 --- Calcium signalling of growth hormone secretagogues --- p.12 / Chapter 1.5 --- Pancreas and functions of exocrine pancreas --- p.15 / Chapter 1.6 --- Regulation of exocrine pancreatic function --- p.22 / Chapter 1.7 --- Functions of ghrelin in pancreas --- p.32 / Chapter 1.8 --- Aims of study --- p.34 / Chapter Chapter 2 --- Materials and methods --- p.35 / Chapter 2.1 --- Experimental animal and cell line models --- p.35 / Chapter 2.1.1 --- Rat model --- p.35 / Chapter 2.1.2 --- Isolation of pancreatic lobules and acinar cells --- p.35 / Chapter 2.1.3 --- Omeprazole-induced gastric acid inhibition --- p.36 / Chapter 2.1.4 --- Cerulein-induced acute pancreatitis --- p.37 / Chapter 2.1.5 --- Starvation rat model --- p.37 / Chapter 2.1.6 --- AR42J cell line --- p.38 / Chapter 2.2 --- Reverse transcriptase-polymerase chain reaction --- p.39 / Chapter 2.2.1 --- Total RNA extraction and quantification --- p.39 / Chapter 2.2.2 --- Reverse transcription --- p.40 / Chapter 2.2.3 --- Polymerase chain reaction --- p.40 / Chapter 2.2.4 --- Gel electrophoresis --- p.41 / Chapter 2.2.5 --- Optimization of semi-quantitative RT-PCR --- p.42 / Chapter 2.3 --- Western blot analysis --- p.43 / Chapter 2.3.1 --- Extraction of total protein and quantification --- p.43 / Chapter 2.3.2 --- Sodium Dodecyl-sulphate Polyacrylamide Gel Electrophoresis (SDS-PAGE) --- p.43 / Chapter 2.3.3 --- Tricine Sodium Dodecyl-sulphate Polyacrylamide Gel Electrophoresis (Tricine-SDS-PAGE) --- p.44 / Chapter 2.3.4 --- Electroblotting and immunodetection of proteins --- p.45 / Chapter 2.4 --- Immunocytochemistry --- p.47 / Chapter 2.4.1 --- Preparation of isolated acinar cells for paraffin sections --- p.47 / Chapter 2.4.2 --- Preparation of AR42J cells slices --- p.47 / Chapter 2.4.3 --- Immunofluorescent double staining --- p.47 / Chapter 2.5 --- Functional studies of digestive enzyme secretion from pancreatic isolated acini and lobules --- p.49 / Chapter 2.5.1 --- Incubation of pancreatic isolated acini and lobules with different peptides --- p.49 / Chapter 2.5.2 --- Quantification of protein content --- p.50 / Chapter 2.5.3 --- Measurement of a-amylase secretion by α-amylase assay --- p.50 / Chapter 2.6 --- Spectrofluorimetric measurement --- p.52 / Chapter 2.6.1 --- Incubation of AR42J cells with Fluo-4/AM --- p.52 / Chapter 2.6.2 --- Pretreatment of antagonist or blockers and Ca2+-free treatment --- p.52 / Chapter 2.6.3 --- Calcium mobilization assay --- p.53 / Chapter 2.7 --- Statistics and data analysis --- p.54

Made available in DSpace on 2014-06-11T19:30:52Z (GMT). No. of bitstreams: 0 Previous issue date: 2003Bitstream added on 2014-06-13T18:41:02Z : No. of bitstreams: 1 kuczynski_lb_dr_botfm.pdf: 2548402 bytes, checksum: ff27d7551d7a419be61cd511e4dedf1e (MD5) / Nas ressecções pancreáticas por pancreatite crônica, o pâncreas remanescente poderá sofrer novos surtos de pancreatite, em gravidade variável. Nas ressecções por tumores ou pancreatite, a mais comum complicação é a fístula pancreática, com suas conseqüentes morbidade e mortalidade. Nos transplantes pancreáticos, a drenagem da parte exócrina do enxerto para o intestino ou para a bexiga, além das peculiaridades técnicas da execução, não é isenta de diversas complicações. Visando evitar ou atenuar tais conseqüências e simplificar a técnica cirúrgica, têm sido usadas outras abordagens para o tratamento do coto/enxerto pancreático: drenagem livre das secreções com o ducto pancreático aberto, ligadura ductal e oclusão do ducto com polímeros. O propósito de nossa investigação foi estudar os efeitos clínicos, laboratoriais e histopatológicos destes procedimentos sobre os componentes endócrino e exócrino do pâncreas em coelhos... / After pancreatic ressections due to chronic pancreatitis the remnant pancreas can lead to new outbreaks of pancreatitis in variable degrees of severity. After the resections by tumors or pancreatitis, the most common complication are the pancreatic fistulas with their resultant morbidity and mortality. In the pancreas transplantation the bowel or bladder drainage of the exocrine part of the graft, beyond the technical peculiarities of the execution, is not exempt of several complications. In order to avoid or reduce such consequences and trying to simplify the surgical technique, there have been used other approaches for the treatment of the pancreatic stump/graft: free drainage of the secretions with the duct left open, ductal ligature and duct occlusion with polymers. The proposal of our investigation was to study the clinical, laboratorial and histopathological effects of these procedures in the endocrine and exocrine compounds of the rabbit pancreas... (Complete abstract, click electronic address below)

Ng, Ka Yan. / Thesis (M.Phil.)--Chinese University of Hong Kong, 2011. / Includes bibliographical references (leaves 179-204). / Abstracts in English and Chinese. / Abstract --- p.I / 摘要 --- p.IV / Publications --- p.VII / Acknowledgements --- p.VIII / Table of contents --- p.IX / List of figures --- p.XV / List of tables --- p.XVII / List of abbreviations --- p.XVIII / Chapter Chapter 1 --- General Introduction / Chapter 1.1 --- The Pancreas --- p.2 / Chapter 1.1.1 --- Anatomy of Pancreas --- p.2 / Chapter 1.1.2 --- The Exocrine Pancreas --- p.4 / Chapter 1.1.3 --- The Endocrine Pancreas --- p.5 / Chapter 1.1.3.1 --- Structure of Islets --- p.5 / Chapter 1.1.3.2 --- "Functions of α-, β-, y-, ð-, Σ-and PP-cells in Islets" --- p.7 / Chapter 1.1.4 --- Overview of Pancreas Development --- p.9 / Chapter 1.1.4.1 --- Organ Morphology --- p.10 / Chapter 1.1.4.2 --- Cyto-differentiation --- p.12 / Chapter 1.1.4.3 --- Control by Transcriptional Factors --- p.14 / Chapter 1.1.5 --- Postnatal Pancreas Development and Regeneration --- p.18 / Chapter 1.1.5.1 --- Proliferation of Pre-existing β-cells --- p.19 / Chapter 1.1.5.2 --- Neogenesis from Precursor Cells --- p.20 / Chapter 1.1.5.3 --- Transdifferentiation of other Cells --- p.20 / Chapter 1.2 --- Diabetes Mellitus --- p.22 / Chapter 1.2.1 --- Pathophysiology of Diabetes Mellitus and Current Treatments --- p.24 / Chapter 1.2.1.1 --- Type I Diabetes Mellitus --- p.24 / Chapter 1.2.1.2 --- Type II Diabetes Mellitus --- p.25 / Chapter 1.2.1.3 --- Gestational Diabetes --- p.27 / Chapter 1.2.1.4 --- Secondary Diabetes --- p.28 / Chapter 1.3 --- Stem Cell therapy --- p.29 / Chapter 1.3.1 --- Stem Cell --- p.29 / Chapter 1.3.1.1 --- Mesenchymal Stem Sell --- p.31 / Chapter 1.3.1.2 --- Embryonic Stem Cell --- p.35 / Chapter 1.3.1.3 --- Induced Pluripotent Stem Cell --- p.36 / Chapter 1.3.2 --- Islets Engineering --- p.37 / Chapter 1.3.2.1 --- Genetic Modification --- p.37 / Chapter 1.3.2.2 --- Directed Differentiation --- p.38 / Chapter 1.3.2.3 --- Microenvironment --- p.38 / Chapter 1.3.2.4 --- In vivo Regeneration --- p.39 / Chapter 1.3.2.5 --- Cell Fusions --- p.40 / Chapter 1.3.2.6 --- Combinatory Treatments --- p.40 / Chapter 1.4 --- The Vitamin A & Vitamin D System --- p.42 / Chapter 1.4.1 --- The Vitamin A --- p.42 / Chapter 1.4.2 --- Vitamin A Metabolism --- p.44 / Chapter 1.4.3 --- Roles of vitamin A in Pancreatic Development --- p.46 / Chapter 1.4.4 --- The Vitamin D --- p.48 / Chapter 1.4.5 --- Vitamin D Metabolism --- p.49 / Chapter 1.4.6 --- Metabolic Functions of Vitamin D in Islets --- p.51 / Chapter 1.4.7 --- Cod Liver Oil --- p.53 / Chapter 1.4.8 --- Interactions between Vitamin A and Vitamin D --- p.53 / Chapter 1.5 --- The Relations of Liver and Pancreas Development --- p.55 / Chapter 1.5.1 --- Endoderm Induction for Hepatic and Pancreatic Differentiation of ESCs --- p.55 / Chapter 1.5.2 --- Bipotential Precursor Population within Embryonic Endoderm --- p.56 / Chapter 1.5.3 --- Pancreatic Islets Promote Mature Liver Hepatocytes Proliferation --- p.57 / Chapter 1.5.4 --- Transdifferentiation --- p.57 / Chapter 1.5.5 --- Transplantation in Liver Niche Promotes Maturation of Insulin-Producing Cells --- p.60 / Chapter 1.5.6 --- Neuronal Relay from the Liver to Pancreatic --- p.61 / Chapter 1.5.7 --- Development of Islets in the Nile Tilapia --- p.62 / Chapter 1.6 --- The Insulin-like Growth Factor-I (IGF1) --- p.64 / Chapter 1.6.1 --- IGF1 System --- p.64 / Chapter 1.6.2 --- IGF 1 Regulation --- p.65 / Chapter 1.6.3 --- Roles of IGF 1 in Pancreatic Development and Regeneration --- p.68 / Chapter 1.7 --- Aims and Objectives of Study --- p.70 / Chapter Chapter 2 --- General Materials and Methods / Chapter 2.1 --- Pancreatic progenitor cells (PPCs) and liver stromal cells (LSCs) isolation and cell culture --- p.72 / Chapter 2.1.1 --- Tissue procurement --- p.72 / Chapter 2.1.2 --- PPC and LSC culture --- p.72 / Chapter 2.1.3 --- "Treatments of vitamin A, vitamin D and IGF 1" --- p.76 / Chapter 2.1.4 --- "Cell culture of Caco-2, HepG2 and DU-145" --- p.76 / Chapter 2.2 --- Induction of Islet-like Cell Clusters (ICCs) Differentiation --- p.77 / Chapter 2.2.1 --- In vitro Directed Differentiation --- p.77 / Chapter 2.2.2 --- In vitro LSC Microenvironment --- p.77 / Chapter 2.3 --- RNA Expression Detection --- p.79 / Chapter 2.3.1 --- RNA isolation --- p.79 / Chapter 2.3.2 --- Reverse Transcription --- p.79 / Chapter 2.3.3 --- Polymerase Chain Reaction (PCR) --- p.80 / Chapter 2.3.4 --- Realtime PCR --- p.81 / Chapter 2.4 --- Immunocytochemistry --- p.83 / Chapter 2.5 --- Western Blotting --- p.85 / Chapter 2.5.1 --- Protein extraction and quantification --- p.85 / Chapter 2.5.2 --- Western Blotting --- p.85 / Chapter 2.6 --- Enzyme-linked Immunosorbent Assay (ELISA) --- p.87 / Chapter 2.6.1 --- Detection of cell viability --- p.87 / Chapter 2.6.2 --- Detection of cell proliferation --- p.87 / Chapter 2.6.3 --- Measurement of Cell death --- p.88 / Chapter 2.6.4 --- Measurement of IGF 1 level in condition medium --- p.89 / Chapter 2.6.5 --- Measurement of glucose induced insulin secretion --- p.90 / Chapter 2.7 --- Regeneration model --- p.92 / Chapter 2.7.1 --- Regeneration model in neonatal-STZ rat --- p.92 / Chapter 2.7.2 --- Change in IGF1 expression in pancreas and liver --- p.92 / Chapter 2.8 --- Statistical Data Analysis --- p.93 / Chapter Chapter 3 --- Vitamin D and vitamin A receptor expression and the proliferative effects of ligand activation of these receptors on the development of pancreatic progenitor cells derived from human fetal pancreas. (Stem Cell Rev. 2011;7:53-63) / Chapter 3.1 --- Abstract --- p.95 / Chapter 3.2 --- Introduction --- p.97 / Chapter 3.3 --- Materials and Methods --- p.101 / Chapter 3.3.1 --- Fetal Tissue Procurement --- p.101 / Chapter 3.3.2 --- Culture of Pancreatic Progenitor Cells --- p.101 / Chapter 3.3.3 --- RNA Expression Analysis by Reverse Transcription-Polymerase Chain Reaction (RT-PCR) --- p.102 / Chapter 3.3.4 --- Western Blot Analysis --- p.103 / Chapter 3.3.5 --- Immunocytochemstry --- p.105 / Chapter 3.3.6 --- PPC Proliferation Assays --- p.106 / Chapter 3.3.7 --- PPC Cell Death Assays --- p.107 / Chapter 3.3.8 --- Statistical Data Analysis --- p.108 / Chapter 3.4 --- Results --- p.110 / Chapter 3.4.1 --- "Expression and Localization of RAR, VDR and RXR, CYP26 and CYP24 in PPCs" --- p.110 / Chapter 3.4.2 --- Incubation of PPC with atRA Enhances PPC Viability due to Increased Proliferation and Anti-apoptosis --- p.111 / Chapter 3.4.3 --- Incubation of PPCs with Calcitriol Enhances Viability due to Increased Proliferation --- p.111 / Chapter 3.4.4 --- Both atRA and Calcitriol Induce Up-regulation of both the RAR and the VDR but not the RXR --- p.112 / Chapter 3.4.5 --- Combination Treatment with atRA and Calcitriol on Cell Viability and NGN3 Expression --- p.112 / Chapter 3.5 --- Discussion --- p.114 / Chapter Chapter 4 --- Human fetal liver stromal cell co-culture enhances the growth and differentiation of pancreatic progenitor cells into islet-like cell clusters (In submission to Gastroenterology) / Chapter 4.1 --- Abstract --- p.128 / Chapter 4.2 --- Introduction --- p.129 / Chapter 4.3 --- Materials and Methods --- p.133 / Chapter 4.3.1 --- Use of human and animal tissues --- p.133 / Chapter 4.3.2 --- "Cell preparation, characterizations and Differentiation" --- p.133 / Chapter 4.3.3 --- Examination of PPC growth and ICC differentiation and functions with LSC co-culture --- p.133 / Chapter 4.3.3 --- Identification of growth factors and investigation of their effects --- p.134 / Chapter 4.3.4 --- Statistical Analysis --- p.135 / Chapter 4.4 --- Results --- p.136 / Chapter 4.4.1 --- "Isolation, Culture and Characterizations of LSCs" --- p.136 / Chapter 4.4.2 --- Establishment of LSC co-culture system --- p.136 / Chapter 4.4.3 --- LSC co-culture enhances PPC-derived ICC differentiation --- p.137 / Chapter 4.4.4 --- Differential expression of mRNA for cytokines and growth factors between 1st and 2nd trimester LSCs --- p.138 / Chapter 4.4.5 --- Characterization of IGF 1 receptors in PPCs and the effects of exogenous IGF1 on PPC growth and ICC differentiation --- p.139 / Chapter 4.4.6 --- Neutralizing antibodies against IGF1R inhibit ICC differentiation --- p.140 / Chapter 4.5 --- Discussion --- p.142 / Chapter 4.6 --- Supplementary Materials and Methods --- p.147 / Chapter 4.6.1 --- Cell Preparation and culture --- p.147 / Chapter 4.6.2 --- In Vitro ICC differentiation --- p.148 / Chapter 4.6.3 --- RNA expression analysis --- p.149 / Chapter 4.6.4 --- Immunocytochemistry --- p.149 / Chapter 4.6.5 --- PPC viability and cell count assays --- p.150 / Chapter 4.6.6 --- IGF1 and insulin ELISA --- p.151 / Chapter 4.6.7 --- Western blotting analysis --- p.152 / Chapter 4.6.8 --- Neonatal streptozotocin regeneration model --- p.153 / Chapter Chapter 5 --- General Discussion and Future Studies / Chapter 5.1 --- General Discussion --- p.165 / Chapter 5.1.1 --- Proliferative effects and enhance expression of NGN3 by vitamin A and vitamin D on PPC --- p.166 / Chapter 5.1.2 --- Induction of PPC derived ICCs by LSCs --- p.169 / Chapter 5.1.3 --- Potential effects of liver stroma derived IGF1 on PPC derived ICCs differentiation --- p.172 / Chapter 5.1.4 --- Significance of islet engineering in the management of diabetes --- p.174 / Chapter 5.1.5 --- Conclusions --- p.176 / Chapter 5.2 --- Future Studies --- p.177 / Chapter Chapter 6 --- Reference / Reference --- p.180

Diss. (sammanfattning) Linköping : Univ., 2004. / Härtill 4 uppsatser.

Thesis (Ph.D.)--University of Alabama at Birmingham, 2007. / Title from PDF title page (viewed on Sept. 15, 2009). Includes bibliographical references.

Thesis (Ph.D.) - University of Queensland, 2003. / Includes bibliography.

Orientadores: Elinton Adami Chaim, José Carlos Pareja / Dissertação (mestrado) - Universidade Estadual de Campinas, Faculdade de Ciências Médicas / Made available in DSpace on 2018-08-16T22:56:32Z (GMT). No. of bitstreams: 1 Gestic_MartinhoAntonio_M.pdf: 3381516 bytes, checksum: 2ac6e6bb2938cf49388dd92cfe4fb63c (MD5) Previous issue date: 2010 / Resumo: O tratamento cirúrgico da pancreatite crônica é indicado na falência do tratamento clínico da dor e na presença de complicações da doença. O emprego da melhor técnica ainda é um desafio e, ao longo do último século, várias técnicas foram desenvolvidas determinando três padrões de procedimento: descompressivos, ressecativos e mistos. A técnica de Frey é do tipo mista, recentemente desenvolvida e que apresenta excelentes resultados no alívio da dor secundária à pancreatite crônica. Seu princípio propõe baixas taxas de morbidade e mortalidade pós-operatórias e menor dano às funções pancreáticas comparáveis às cirurgias descompressivas (Partington-Rochelle, Puestow) com a mesma efetividade das cirurgias ressecativas (duodenopancreatectomias) no controle da dor. O objetivo deste trabalho é descrever a casuística e analisar os resultados de uma série consecutiva de pacientes com pancreatite crônica submetidos à técnica de Frey no Hospital de Clínicas da UNICAMP. Foram analisados retrospectivamente 73 pacientes consecutivos de janeiro de 1991 a dezembro de 2007, sem tratamento cirúrgico prévio para pancreatite crônica e com pelo menos um ano de seguimento pós-operatório. Estudou-se o perfil da população, indicação cirúrgica, complicações pós-operatórias e resultados a longo prazo no controle da dor e das complicações. Os pacientes apresentaram idade média de 40,6 anos, sendo a maioria homens (97,3%) e a etiologia alcoólica foi a mais freqüente (95,9%). A dor abdominal acometia todos os pacientes, 98,8% com intensidade moderada ou severa. A taxa de morbidade global foi de 28,7% e as complicações mais freqüentes foram as infecciosas (13,7%), dentre elas as pneumonias; a prevalência de fístulas da anastomose pancreática foi de 6,8%. Não houve mortalidade cirúrgica. Em seguimento médio de 77,0 meses, 91,4% dos pacientes apresentavam remissão dolorosa completa e houve aumento do IMC no pós-operatório (p < 0,001). Insuficiência exócrina nova apareceu em 49% dos pacientes e diabetes de novo em 36,7%. A recidiva de ingestão alcoólica ocorreu em 32,9% dos pacientes, os quais apresentaram menor expectativa de vida em relação àqueles que se mantiveram abstêmios (p = 0,02). As principais causas de mortalidade tardia foram as neoplasias do trato aéreo-digestivo superior e complicações de cirrose hepática. Identificaram-se associações estatisticamente significativas entre abstinência alcoólica pré-operatória com menor taxa de complicações infecciosas e fistulas; a quantidade de ingestão de álcool e o tempo de aparecimento de diabetes nova pós-operatória; o calibre do ducto pancreático com o surgimento de diabetes pós-operatória; e níveis elevados de amilase sérica e no liquido do dreno abdominal no primeiro dia pós-operatório com fístulas. A técnica de Frey mostrou-se uma opção segura e eficaz para o tratamento cirúrgico da pancreatite crônica, proporciononando melhora da sintomatologia dolorosa e reganho de peso e não interrompeu a deterioração das funções exócrina e endócrina do pâncreas. A recidiva do abuso da ingestão de etanol é um problema freqüente nesses pacientes e interfere na sobrevida deles / Abstract: Surgical treatment of chronic pancreatitis is indicated for failure in clinical pain management of the disease. Frey's surgery is one of the techniques available for intervention in this process. Application of the best technique is still a challenge today and, over the last century, several techniques were developed by determining three patterns of treatment: decompression, resection and mixed. Frey's procedure is a mixed technique, which was recently developed and shows excellent results in pain relief. Its principle suggests low rates of morbidity and mortality after surgery and less damage to pancreatic function comparable to surgical decompression (Partington-Rochelle, Puestow) with the same effectiveness of the resection procedures (pancreatoduodenectomy) in pain control. The aim of this paper is to describe and analyze the results of a consecutive series of patients with chronic pancreatitis underwent Frey technique at the Hospital de Clínicas of UNICAMP. Seventy-three consecutive patients were retrospectively analyzed from January 1991 to December 2007, with no previous surgical treatment for chronic pancreatitis and with at least one year of follow-up. We studied the profile of the population, surgical indication, postoperative complications and long-term results in controlling pain and complications. Patients had mean age of 40.6 years, most were men (97.3%) and alcoholic etiology was the most frequent (95.9%). Abdominal pain gripped all patients, 98.8% with moderate or severe intensity. The overall morbidity rate was 28.7% and the most frequent complications were infections (13.7%), among them pneumonia. Fistulas of pancreatic anastomosis were 6.8%. There was no surgical mortality. At mean follow-up of 77.0 months, 91.4% of patients had complete pain remission and there was increased of BMI postoperatively (p < 0.001). New exocrine insufficiency appeared in 49% and new diabetes in 36.7%. Recurrence of alcohol consumption occurred in 32.9% of patients, which showed a lower life expectancy than those who remained abstinent (p = 0.02). The main causes of late death were neoplasm of the upper aero-digestive tract and complications of liver cirrhosis. We identified significant associations between preoperative alcohol abstinence with a lower rate of infectious complications and fistulas; the amount of alcohol intake and time of onset of postoperative diabetes; diameter of the pancreatic duct with the onset of postoperative diabetes; and elevated levels of amylase in blood and abdominal drain fluid on the first postoperative day with fistulas. Frey procedure proved to be a safe and effective option for the surgical treatment of disabling pain caused by chronic pancreatitis provided regained weight but did not stop the deterioration of exocrine and endocrine functions of the pancreas. Recurrence of ethanol abuse is a frequent problem in these patients and interferes with their life expectancy / Mestrado / Fisiopatologia Cirúrgica / Mestre em Ciências da Cirurgia

Le cancer du pancréas est un problème majeur de santé publique. Le mauvais pronostic de l'adénocarcinome du pancréas est lié à un diagnostic tardif, une progression rapide, et à une mauvaise réponse aux traitements médicaux disponibles. Une caractérisation complète des altérations génétiques moléculaires sont aujourd'hui nécessaires pour permettre une détection plus précoce et identifier/élaborer de nouvelles thérapies ciblées dans le traitement de l'ADK du pancréas. En utilisant la technique d'hybridation génomique comparative, nous avons étudié les altérations du génome de 39 ADK. Plusieurs pertes récurrentes ont été observées, ainsi que des gains de matériel génétique. A partir de ces résultats, nous avons voulu aller plus loin en identifiant les gènes qui pourraient avoir des conséquences au niveau transcriptomique. A partir de données publiques, nous avons comparé les profils d'expression d'ADK (n=419) et de pancréas normal (n=105). Parmi les gènes trouvés amplifiés et/ou gagnés par aCGH, 170 (48%) étaient surexprimés dans les ADK par rapport au tissus normal. Les principales voies de signalisation impliquées touchaient la régulation du cycle cellulaire, les voies TP53 et TGFß. Parmi les gènes délétés en aCGH, 141 (41%) étaient sous exprimés dans les ADK du pancréas par rapport au tissus normal et étaient essentiellement liés au « métabolome » de la cellule pancréatique. Enfin, nous avons identifié une dizaine de gènes dont l'expression pourrait être liée à la survie des patients. Certains de ces gènes pourraient être des candidats à tester en tant que biomarqueurs pronostic ou cibles pour le développement de nouvelles thérapeutiques. / Pancreatic adenocarcinoma (PDCA) is a major public health problem in France and worldwide. The inoperability and the poor prognosis of the PDCA are due to late diagnosis, rapid tumor progression (>80% of patients displayed metastases at diagnosis), early recurrences after resection, and poor response to available therapies. Innovative approaches and a comprehensive characterization of molecular genetic alterations are dearly needed to help develop techniques of early detection, identify new molecular targets and devise novel targeted-therapies (Hidalgo, 2010). Using high-resolution array-comparative genomic hybridization (aCGH), we studied the genome alterations of 39 fine-needle aspirations from PDCA. Recurrent losses were observed and comprised several known tumor suppressor genes. We identified frequent genetic gains. With this study, we decided to go one step further by identifying genes that might also be deregulated at the transcriptomic level. We started our analysis with a population of PDCA (n=419) versus normal pancreas (n = 105). Among the 352 genes found amplified and/or gained by aCGH, 170 (48%) were up regulated at the transcriptional level in PDCA compared to normal pancreatic tissues. Major pathways involved were cell cycle, TP53 and TGFß. Among the genes located in regions of losses, 141 (41%) were down regulated in PDCA compared to normal tissues. Furthermore, some genes were found related to a patients' survival With this study, we highlighted novels genes associated to PDCA oncogenesis. Some of those candidates should be further investigated as prognosis markers or as potential targets for new therapeutic approaches.