Boron neutron capture therapy (BNCT) has the potential to specifically destroy tumor cells without damaging the tissues infiltrated by the tumor. BNCT is a binary treatment method based on the combination of two agents that have no effect when applied individually: 10B and thermal neutrons. Exclusively, the combination of both produces an effect, whose extent depends on the amount of 10B in the tumor but also on the organs at risk. It is not yet possible to determine the 10B concentration in a specific tissue using non-invasive methods. At present, it is only possible to measure the 10B concentration in blood and to estimate the boron concentration in tissues based on the assumption that there is a fixed uptake of 10B from the blood into tissues. On this imprecise assumption, BNCT can hardly be developed further. A therapeutic approach, combining the boron carrier for therapeutic purposes with an imaging tool, might allow us to determine the 10B concentration in a specific tissue using a non-invasive method. This review provides an overview of the current clinical protocols and preclinical experiments and results on how innovative drug development for boron delivery systems can also incorporate concurrent imaging. The last section focuses on the importance of proteomics for further optimization of BNCT, a highly precise and personalized therapeutic approach.

No description available.

Near field imaging using microwave in medical applications has gain much attention recently as various researches show its high ability and accuracy in illuminating object comparing to the well-known screening tools such as Magnetic Resonance Imaging (MRI), digital mammography, ultrasound etc. This has encourage and motivate scientists continue to exploit the potential of microwave imaging so that a better and more powerful sensing tools can be developed. This thesis documents the development of antenna design for microwave imaging application such as breast cancer detection. The application is similar to the concept of Ground Penetrating Radar (GPR) but operating at higher frequency band. In these systems a short pulse is transmitted from an antenna to the medium and the backscattered response is investigated for diagnose. In order to accommodate such a short pulse, a very wideband antenna with a minimal internal reflection is required. Printed monopole and planar metal plate antenna is implemented to achieve the necessary operating wide bandwidth. The development of new compact printed planar metal plate ultra wide bandwidth antenna is presented. A generalized parametric study is carried out using two well-known software packages to achieve optimum antenna performance. The Prototype antennas are tested and analysed experimentally, in which a reasonable agreement was achieved with the simulations. The antennas present an excellent relative wide bandwidth of 67% with acceptable range of power gain between 3.5 to 7 dBi. A new compact size air-dielectric microstrip patch-antenna designs proposed for breast cancer detection are presented. The antennas consist of a radiating patch mounted on two vertical plates, fed by coaxial cable. The antennas show a wide bandwidth that were verified by the simulations and also confirmed experimentally. The prototype antennas show excellent performance in terms the input impedance and radiation performance over the target range bandwidth from 4 GHz to 8 GHz. A mono-static model with a homogeneous dielectric box having similar properties to human tissue is used to study the interaction of the antenna with tissue. The numerical results in terms the matching required of new optimised antennas were promising. An experimental setup of sensor array for early-stage breast-cancer detection is developed. The arrangement of two elements separated by short distance that confined equivalent medium of breast tissues were modelled and implemented. The operation performances due to several orientations of the antennas locations were performed to determine the sensitivity limits with and without small size equivalent cancer cells model. In addition, a resistively loaded bow tie antenna, intended for applications in breast cancer detection, is adaptively modified through modelling and genetic optimisation is presented. The required wideband operating characteristic is achieved through manipulating the resistive loading of the antenna structure, the number of wires, and their angular separation within the equivalent wire assembly. The results show an acceptable impedance bandwidth of 100.75 %, with a VSWR < 2, over the interval from 3.3 GHz to 10.0 GHz. Feasibility studies were made on the antenna sensitivity for operation in a tissue equivalent dielectric medium. The simulated and measured results are all in close agreement.

Within the archaeological record ‘mounds’ are often ubiquitous. They are common in many ancient cultures, and they vary in size, construction techniques and use. This research is focused upon optimizing the use of remote sensing for the non-invasive study of mounds both in the United States and the United Kingdom. This thesis presents three representative earthen mound sites and proposes a comprehensive and modular survey methodology to guide the planning and execution of a mound survey tailored to the unique requirements presented by the cultural resource at a particular location. In doing so, the research has provided optimized approaches to high resolution three-dimensional topographic models using a variety of digital methods. These models have been shown to accurately capture the variability of the modern ground surface, which is of vital importance to the management of the mounds. Furthermore, these models have proved vital for integrating geophysical methods into the holistic workspace, thereby providing a better archaeological understanding of the below ground remains. Every mound surveyed presented different challenges, and therefore had to be approached in a slightly different way. However, the general methodology was highly effective for both characterizing below-ground archaeological and natural anomalies, and for assessing the state of preservation of all mounds surveyed. As a result, a flowchart has been generated for non-invasive assessment of mounds in general. If followed, this will allow the production of a “snapshot” of the mound or mound group at a fixed point in time with the resolution necessary to produce useful and insightful interpretation. While this research focuses on the application of geophysical and topographic survey in the United Kingdom and United States to a mound or mound group, this methodology and the associated outcomes can be valuable more globally not only for archaeology, but also heritage management.

No description available.

<p>We evaluated 3-D imaging of coarse root structure and biomass using ground-penetrating radar (GPR). GPR surveys were conducted in a white pine forest in southern Ontario, Canada. GPR profiles were obtained across two test plots (6 and 17 m² area), using 1-GHz GPR and a MEMS (micro-electro-mechanical systems) accelerometer. Test plot surveys evaluated the effects of micro-topography, soil moisture content, and root diameter and spacing. In addition, with the aid of the outcome of the control test plots two other plots (25 and 400 m²area) were surveyed with varying line sample spacing to investigate the restraints on resolution brought about by line sampling density.</p> <p>Accounting for antenna tilt is necessary to determine an accurate and more precise position of root mass. The antenna tilt was >45^o pitch, >28^o roll and up to 10^o yaw due to surface micro-topography of the forest floor. Vector 3-D imaging enhanced the diffraction amplitude (15.5% increase) and centralized the position of the root. Radial surveys provided root continuity and produced better root imaging.</p> <p>GPR largely underestimates coarse root biomass when a line spacing of 25 cm is used. However similar results are found with smaller line spacing (12.5 cm). A maximum line spacing of 10 cm provided continuous root structure and differentiation of roots spaced 10 cm apart and greater. A sampling line spacing of 5 cm and an inline sampling interval of 0.5 cm in low soil moisture conditions provided the detection of roots that were a minimum of 1.4 cm in diameter.</p> / Master of Science (MSc)

This thesis investigates the application of In-Situ Recycling and provides guidelines for localities to aid in the selection of recycling methods, as well as documents a local government's experience with Cold In-Place Recycling. The recycling methods discussed in this study include Cold In-Place Recycling (CIR), Hot In-Place Recycling (HIR), and Full Depth Reclamation (FDR). These methods are performed onsite and in-place in a continuous process of milling, mixing, and placement. The In-Situ Recycling guidelines include suggestions based on: traffic characteristics, existing road condition, distress types, road access, local climate, road geometry, and other road characteristics. The guidelines are based on information from sources including NCHRP Synthesis 421, American Recycling and Reclamation Association (ARRA), FHWA, and state agencies with recycling experience. This study also resulted in documenting obstacles that localities may face when in-situ recycling, as well as the impact of limited experience with recycling. The study also evaluated the construction of Cold In-Place Recycled pavement sections in Christiansburg, VA, using Falling Weight Deflectometer (FWD) and Ground Penetrating Radar (GPR). Additionally, using the FWD and GPR data, alternate recycled designs were proposed in addition to a cost comparison to a conventional design. / Master of Science

Nesta pesquisa são apresentadas as contribuições dos métodos GPR e eletromagnético indutivo (equipamento EM-38) nos estudos de sítios arqueológicos de sambaquis costeiros Jabuticabeira II, Santa Marta IV, V, VII e VIII, e Encantada III, localizados no município de Jaguaruna, litoral centro-sul de Santa Catarina. Estes sítios são caracterizados por acúmulos de conchas carbonáticas construídos por sociedades do período pré-colonial (7,5 a 1,3 mil anos AP). Os estudos foram desenvolvidos, visando o mapeamento de artefatos de interesse arqueológico e de estruturas estratigráficas que auxiliem a compreensão do processo construtivo e funcional destes sítios. A interpretação dos resultados GPR foi apoiada nas modelagens numéricas GPR 2D e nas imagens 3D e integrada com os levantamentos EM-38, e tiveram como objetivos orientar as escavações arqueológicas. Os resultados permitiram encontrar alvos e artefatos arqueológicos, reduziram os custos no processo exploratório e preservaram o patrimônio histórico. Complementando o processo de interpretação integrada, os perfis estratigráficos e as análises granulométricas dos sedimentos provenientes dos furos de sondagens foram importantes para a definição dos ambientes de deposição onde os sambaquis estão assentados, corroborando de maneira significativa com as pesquisas em desenvolvimento no litoral de Santa Catarina. Em termos metodológicos, as antenas GPR blindadas de 200 MHz propiciaram um melhor compromisso entre a profundidade de investigação e a resolução vertical das camadas geo-arqueológicas, e a implementação da técnica de aquisição radial permitiu um mapeamento detalhado do sítio Jabuticabeira II de forma rápida, cobrindo uma grande área. Por outro lado, com o método eletromagnético indutivo (EM-38), a correção do efeito topográfico dos dados melhorou os contrastes de condutividade elétrica entre as estruturas arqueológicas e o background, permitindo assim, que os alvos pontuais, antes mascarados pela influência da topografia, fossem realçados. Com relação aos resultados geofísicos em estudos geoarqueológicos, no sambaqui Jabuticabeira II, foi possível caracterizar a geometria de uma estrutura geológica, associada a um paleo-canal e a deposição dos sedimentos em barras de pontal; mapear alvos arqueológicos e metálicos contemporâneos; traçar os limites do sítio; imagear uma camada conchífera, camadas antrópicas recentes e a profundidade do nível dágua; detectar a presença de dois sistemas deposicionais, paleolaguna e paleoduna, bem como delimitar o assentamento do sítio sobre estes ambientes por meio das informações das análises granulométricas dos sedimentos coletados nos furos de sondagens. Nos sambaquis de Santa Marta IV, V, VII e VIII, a integração dos dados GPR e EM38 permitiram o mapeamento de diversos alvos de grande importância para os estudos arqueológicos, tais como, paleofogueiras, sepultamentos e concentração de materiais cerâmicos e líticos, bem como feições geológicas, tais como, estruturação de camadas e paleotômbolos. Além disso, a redução do efeito topográfico sobre os dados de condutividade elétrica (EM-38) permitiu relacionar as regiões anômalas com um paleofogueira e uma concentração de material cerâmico. No sambaqui Encantada III, duas fortes anomalias GPR, caracterizadas por reflexões hiperbólicas, estavam associadas: i) a uma estrutura escura pontual, caracterizada como um bolsão de conchas carbonáticas; e ii) a presença de uma raiz de árvore concrecionada, que embora não seja de interesse arqueológico, é significativa, pois serve como um bom exemplo de ambiguidade na interpretação de dados geofísicos. Também foi possível delimitar o assentamento do sítio sobre os sedimentos da paleo-laguna, evidenciado pelas análises granulométricas dos sedimentos. / In this study, GPR and electromagnetic induction (EM-38 instrument) methodcontributions in coastal sambaqui archaeological sites (Jabuticabeira II, Santa Marta IV, V, VII and VIII as well as Encantada III) are presented. These sites are placed in Jaguaruna, Santa Catarina center-south coast. They are characterized by accumulation of carbonate shells built by societies in pre-colonial period (7.5 to 1.3 thousand years BP). The studies were developed aimed at mapping archaeological artifacts and stratigraphic structures that help to understand constructive and functional process of these sites. The interpretation of GPR results was supported by 2D GPR numerical modeling, 3D images and integrated with EM-38 surveys. They had as objectives to guide archaeological excavations. The results allowed finding archaeological targets and artifacts, reduced costs in exploratory process, and preserved historical heritage. Complementing integrated interpretation process, stratigraphic profiles and granulometric analysis of sediment from sounding drifts were important for defining the deposition environments where sambaquis (shell mounds) are settled, significantly supporting in developing research on Santa Catarina coast. Methodologically, 200 MHz shielded GPR antennas provided a better agreement between depth of investigation and vertical resolution of geoarchaeological layers, and the implementation of radial acquisition technique allowed a quickly detailed mapping of Jabuticabeira II site, covering a large area. Furthermore, with electromagnetic inductive method (EM-38), the topographic effect correction of data has improved the contrast in electrical conductivity between archaeological structures and background. Thus, punctual targets before masked by topography influence were highlighted. Regarding geophysical results in geoarchaeological studies, in Jabuticabeira II sambaqui, it was possible to characterize the geometry of a geological structure associated with a paleochannel and sediment deposition in point bars; to map archaeological and contemporary metal targets; to trace site boundary; to image shell layer, recent anthropic layers and water level depth; to detect the presence of two deposicional systems, paleolagoon and paleodune as well as to delimit the settlement site on these environments through information of granulometric analysis of sediments collected in sounding drifts. In Santa Marta IV, V, VII and VIII sambaquis, GPR and EM-38 data integration allowed mapping several targets of great importance for archaeological studies, such as paleofires, burials and concentration of ceramic and litic material as well as geological features, such as layer structuring and paleotombolos. Moreover, the reduction of topographic effect on electrical conductivity data (EM-38) allowed relating anomalous regions with a paleofire, and a concentration of ceramic material. In Encantada III sambaqui, two strong GPR anomalies characterized by hyperbolic reflections were associated with: i) a dark punctual structure, characterized as a pocket of shell carbonate, and ii) the presence of a concretion tree root that is significant, despite not of archaeological interest, because it serves as a good example of ambiguity in geophysical data interpretation. It was also possible to delimit the settlement site on paleolagoon sediments, evidenced by granulometric analysis of sediments.

Nesta pesquisa são apresentadas as contribuições dos métodos GPR e eletromagnético indutivo (equipamento EM-38) nos estudos de sítios arqueológicos de sambaquis costeiros Jabuticabeira II, Santa Marta IV, V, VII e VIII, e Encantada III, localizados no município de Jaguaruna, litoral centro-sul de Santa Catarina. Estes sítios são caracterizados por acúmulos de conchas carbonáticas construídos por sociedades do período pré-colonial (7,5 a 1,3 mil anos AP). Os estudos foram desenvolvidos, visando o mapeamento de artefatos de interesse arqueológico e de estruturas estratigráficas que auxiliem a compreensão do processo construtivo e funcional destes sítios. A interpretação dos resultados GPR foi apoiada nas modelagens numéricas GPR 2D e nas imagens 3D e integrada com os levantamentos EM-38, e tiveram como objetivos orientar as escavações arqueológicas. Os resultados permitiram encontrar alvos e artefatos arqueológicos, reduziram os custos no processo exploratório e preservaram o patrimônio histórico. Complementando o processo de interpretação integrada, os perfis estratigráficos e as análises granulométricas dos sedimentos provenientes dos furos de sondagens foram importantes para a definição dos ambientes de deposição onde os sambaquis estão assentados, corroborando de maneira significativa com as pesquisas em desenvolvimento no litoral de Santa Catarina. Em termos metodológicos, as antenas GPR blindadas de 200 MHz propiciaram um melhor compromisso entre a profundidade de investigação e a resolução vertical das camadas geo-arqueológicas, e a implementação da técnica de aquisição radial permitiu um mapeamento detalhado do sítio Jabuticabeira II de forma rápida, cobrindo uma grande área. Por outro lado, com o método eletromagnético indutivo (EM-38), a correção do efeito topográfico dos dados melhorou os contrastes de condutividade elétrica entre as estruturas arqueológicas e o background, permitindo assim, que os alvos pontuais, antes mascarados pela influência da topografia, fossem realçados. Com relação aos resultados geofísicos em estudos geoarqueológicos, no sambaqui Jabuticabeira II, foi possível caracterizar a geometria de uma estrutura geológica, associada a um paleo-canal e a deposição dos sedimentos em barras de pontal; mapear alvos arqueológicos e metálicos contemporâneos; traçar os limites do sítio; imagear uma camada conchífera, camadas antrópicas recentes e a profundidade do nível dágua; detectar a presença de dois sistemas deposicionais, paleolaguna e paleoduna, bem como delimitar o assentamento do sítio sobre estes ambientes por meio das informações das análises granulométricas dos sedimentos coletados nos furos de sondagens. Nos sambaquis de Santa Marta IV, V, VII e VIII, a integração dos dados GPR e EM38 permitiram o mapeamento de diversos alvos de grande importância para os estudos arqueológicos, tais como, paleofogueiras, sepultamentos e concentração de materiais cerâmicos e líticos, bem como feições geológicas, tais como, estruturação de camadas e paleotômbolos. Além disso, a redução do efeito topográfico sobre os dados de condutividade elétrica (EM-38) permitiu relacionar as regiões anômalas com um paleofogueira e uma concentração de material cerâmico. No sambaqui Encantada III, duas fortes anomalias GPR, caracterizadas por reflexões hiperbólicas, estavam associadas: i) a uma estrutura escura pontual, caracterizada como um bolsão de conchas carbonáticas; e ii) a presença de uma raiz de árvore concrecionada, que embora não seja de interesse arqueológico, é significativa, pois serve como um bom exemplo de ambiguidade na interpretação de dados geofísicos. Também foi possível delimitar o assentamento do sítio sobre os sedimentos da paleo-laguna, evidenciado pelas análises granulométricas dos sedimentos. / In this study, GPR and electromagnetic induction (EM-38 instrument) methodcontributions in coastal sambaqui archaeological sites (Jabuticabeira II, Santa Marta IV, V, VII and VIII as well as Encantada III) are presented. These sites are placed in Jaguaruna, Santa Catarina center-south coast. They are characterized by accumulation of carbonate shells built by societies in pre-colonial period (7.5 to 1.3 thousand years BP). The studies were developed aimed at mapping archaeological artifacts and stratigraphic structures that help to understand constructive and functional process of these sites. The interpretation of GPR results was supported by 2D GPR numerical modeling, 3D images and integrated with EM-38 surveys. They had as objectives to guide archaeological excavations. The results allowed finding archaeological targets and artifacts, reduced costs in exploratory process, and preserved historical heritage. Complementing integrated interpretation process, stratigraphic profiles and granulometric analysis of sediment from sounding drifts were important for defining the deposition environments where sambaquis (shell mounds) are settled, significantly supporting in developing research on Santa Catarina coast. Methodologically, 200 MHz shielded GPR antennas provided a better agreement between depth of investigation and vertical resolution of geoarchaeological layers, and the implementation of radial acquisition technique allowed a quickly detailed mapping of Jabuticabeira II site, covering a large area. Furthermore, with electromagnetic inductive method (EM-38), the topographic effect correction of data has improved the contrast in electrical conductivity between archaeological structures and background. Thus, punctual targets before masked by topography influence were highlighted. Regarding geophysical results in geoarchaeological studies, in Jabuticabeira II sambaqui, it was possible to characterize the geometry of a geological structure associated with a paleochannel and sediment deposition in point bars; to map archaeological and contemporary metal targets; to trace site boundary; to image shell layer, recent anthropic layers and water level depth; to detect the presence of two deposicional systems, paleolagoon and paleodune as well as to delimit the settlement site on these environments through information of granulometric analysis of sediments collected in sounding drifts. In Santa Marta IV, V, VII and VIII sambaquis, GPR and EM-38 data integration allowed mapping several targets of great importance for archaeological studies, such as paleofires, burials and concentration of ceramic and litic material as well as geological features, such as layer structuring and paleotombolos. Moreover, the reduction of topographic effect on electrical conductivity data (EM-38) allowed relating anomalous regions with a paleofire, and a concentration of ceramic material. In Encantada III sambaqui, two strong GPR anomalies characterized by hyperbolic reflections were associated with: i) a dark punctual structure, characterized as a pocket of shell carbonate, and ii) the presence of a concretion tree root that is significant, despite not of archaeological interest, because it serves as a good example of ambiguity in geophysical data interpretation. It was also possible to delimit the settlement site on paleolagoon sediments, evidenced by granulometric analysis of sediments.

Cette thèse porte sur l’étude de l’anatomie de la cornée après 3 techniques de greffe soient, la greffe totale traditionnelle (GTT) et des techniques de greffe lamellaire postérieur (GLP) telles que la greffe lamellaire endothéliale profonde (DLEK) et la greffe endothélium/membrane de Descemet (EDMG) pour le traitement des maladies de l’endothélium, telles que la dystrophie de Fuchs et de la kératopathie de l’aphaque et du pseudophaque. Dans ce contexte, cette thèse contribue également à démontrer l’utilité de la tomographie de cohérence optique (OCT) pour l’étude de l’anatomie des plaies chirurgicales la cornée post transplantation. Au cours de ce travail nous avons étudié l'anatomie de la DLEK, avant et 1, 6, 12 et 24 mois après la chirurgie. Nous avons utilisé le Stratus OCT (Version 3, Carl Zeiss, Meditec Inc.) pour documenter l’anatomie de la plaie. L'acquisition et la manipulation des images du Stratus OCT, instrument qui à été conçu originalement pour l’étude de la rétine et du nerf optique, ont été adaptées pour l'analyse du segment antérieur de l’oeil. Des images cornéennes centrales verticales et horizontales, ainsi que 4 mesures radiaires perpendiculaires à la plaie à 12, 3, 6 et 9 heures ont été obtenues. Les paramètres suivants ont été étudiés: (1) Les espaces (gap) entre les rebords du disque donneur et ceux du receveur, (2) les dénivelés de surface postérieure (step) entre le les rebords du disque donneur et ceux du receveur, (3) la compression tissulaire, (4) le décollement du greffon, 6) les élévations de la surface antérieure de la cornée et 7) la pachymétrie centrale de la cornée. Les mesures d’épaisseur totale de la cornée ont été comparées et corrélées avec celles obtenues avec un pachymètre à ultra-sons. Des mesures d’acuité visuelle, de réfraction manifeste et de topographie ont aussi été acquises afin d’évaluer les résultats fonctionnels. Enfin, nous avons comparé les données de DLEK à celles obtenues de l’EDMG et de la GTT, afin de caractériser les plaies et de cerner les avantages et inconvénients relatifs à chaque technique chirurgicale. Nos résultats anatomiques ont montré des différences importantes entre les trois techniques chirurgicales. Certains des paramètres étudiés, comme le sep et le gap, ont été plus prononcés dans la GTT que dans la DLEK et complètement absents dans l’EDMG. D’autres, comme la compression tissulaire et le décollement du greffon n’ont été observés que dans la DLEK. Ceci laisse entrevoir que la distorsion de la plaie varie proportionnellement à la profondeur de la découpe stromale du receveur, à partir de la face postérieure de la cornée. Moins la découpe s’avance vers la face antérieure (comme dans l’EDMG), moins elle affecte l’intégrité anatomique de la cornée, le pire cas étant la découpe totale comme dans la GTT. Cependant, tous les paramètres d’apposition postérieure sous-optimale et d’élévation de la surface antérieure (ce dernier observé uniquement dans la GTT) finissent par diminuer avec le temps, évoluant à des degrés variables vers un profil topographique plus semblable à celui d’une cornée normale. Ce processus paraît plus long et plus incomplet dans les cas de GTT à cause du type de plaie, de la présence de sutures et de la durée de la cicatrisation. Les valeurs moyennes d’épaisseur centrale se sont normalisées après la chirurgie. De plus, ces valeurs moyennes obtenues par OCT étaient fortement corrélées à celles obtenues par la pachymétrie à ultra-sons et nous n’avons remarqué aucune différence significative entre les valeurs moyennes des deux techniques de mesure. L’OCT s’est avéré un outil utile pour l’étude de l’anatomie microscopique des plaies chirurgicales. Les résultats d’acuité visuelle, de réfraction et de topographie des techniques de GLP ont montré qu’il existe une récupération visuelle rapide et sans changements significatifs de l’astigmatisme, contrairement à la GTT avec et sans suture. La GLP a permis une meilleure conservation de la morphologie de la cornée, et par conséquence des meilleurs résultats fonctionnels que la greffe de pleine épaisseur. Ceci nous permet d’avancer que la GLP pourrait être la technique chirurgicale à adopter comme traitement pour les maladies de l’endothélium cornéen. / This thesis aims to study the anatomy of the corneal wound following 3 techniques of corneal graft: traditional penetrating keratoplasy (PK) and two techniques of posterior lamellar keratoplasy (PLK) which are deep lamellar endothelial keratoplasy (DLEK) and Endothelial-Descemet’s Membrane Graft (EDMG) for the treatment of the endothelial corneal diseases, such as Fuch’s dystrophy and aphakic and pseudopakic bullous keratopathy. In this context, this thesis also contributes to show the utility of the time domain optical coherence tomography (TD-OCT) for studying the anatomy of surgical wounds after corneal transplantation. In this work we studied the anatomy of DLEK, before and 1,6,12 and 24 months after surgery. We used the Stratus OCT. (Version 3, Carl Zeiss, Meditec Inc.) to document the anatomy of the wound. The acquisition and the handling of the images of the Stratus OCT, an instrument originally designed for the study of the retina and the optic nerve, were adapted to analyse the anterior segment of the eye. Vertical and horizontal central images of the cornea, in addition to 4 radial measurements perpendicular to the wound at 12, 3, 6 and 9 hours were obtained. The following parameters were studied: (1) the gap between the edges of the donor disc and those of the recipient, (2) posterior surface mismatch (step) between the edges of the disc donor and those of the recipient, (3) tissue compression, (4) graft detachment, 6) elevations of the anterior corneal surface and 7) the central pachymetry of the cornea. Measurements of the total thickness were compared and correlated with those obtained with an ultrasound pachymeter. Measurements of visual acuity, manifest refraction and topography were also acquired in order to evaluate the functional results. Lastly, we compared the data of DLEK with those obtained from the EDMG and the PK, in order to characterize the wounds and to highlight the advantages and disadvantages relative to each surgical technique.Our anatomical results showed important differences between the three surgical techniques. Some of the studied parameters, like the step and the gap, were more pronounced in PK than in DLEK and completely absent in the EDMG group. Others, like tissue compression and graft detachment were observed only in the DLEK group. This let us predict that the distortion of the wound varies proportionally with the depth of recipient posterior stromal dissection. The less dissection towards the anterior surface (as in EDMG), the less it affects the anatomical integrity of the cornea, the worst case being full thickness trephination as in PK. However, all the parameters of sub-optimal posterior surface apposition and anterior surface elevation (this last only observed in PK) ended up decreasing with time, evolving with variable degrees to a topographic profile more similar to that of a normal cornea. This process appears longer and more incomplete in the cases of PK because of the type of wound, the presence of sutures and the longer healing period. The mean values of central thickness were normal after surgery. Moreover, these mean values obtained by OCT. were strongly correlated with those obtained by ultrasound pachymetry and we did not notice any significant difference between the mean values of the two measurement techniques. OCT proved to be a useful tool for the study of the microscopic anatomy of the corneal surgical wounds. The results of vision, refraction and topography of the techniques of posterior lamellar grafts showed that there was a fast visual recovery and without significant changes in astigmatism, contrary to PK with and without sutures. Posterior lamellar grafts allowed a better conservation of the morphology of the cornea, and consequently better functional results than PK. This enabled us to conclude that posterior lamellar corneal grafts could be the surgical technique of choice for the treatment of corneal endothelial diseases.