Orientador: Marcelo Andreotti / Banca: Élcio Hiroyoshi Yano / Banca: Rogerio Peres Soratto / Resumo: Como alternativa para aumento da produtividade dos sistemas de produção, surgiu o Sistema de Integração Lavoura-Pecuária. Desta maneira, objetivou-se avaliar em um LATOSSOLO VERMELHO Distroférrico: 1) os teores nutricionais e leituras ICF em folhas de milho, assim como os componentes da produção e produtividade de grãos da cultura em consórcio com duas espécies de braquiárias (B. brizantha cv. MG-5 e B. ruziziensis) submetidos a doses de N em cobertura; 2) avaliar a produtividade de fitomassa das espécies forrageiras após a colheita do milho, também submetidas à doses de N, assim como os teores nutricionais, composição bromatológica e a taxa de decomposição da palhada das braquiárias; 3) avaliar os teores nutricionais e leituras ICF em folhas de feijoeiro de inverno cultivado em sucessão, assim como os componentes da produção e produtividade de grãos; e 4) avaliar o desempenho econômico do sistema de ILP, considerando o milho consorciado, a pastagem e o feijão em sucessão. Para tanto, foram conduzidos três experimentos seqüenciais, durante o ano agrícola de 2008/2009 na Fazenda de Ensino, Pesquisa e Extensão, da Faculdade de Engenharia - Unesp, Campus de Ilha Solteira, em condições irrigadas no Cerrado, com histórico de oito anos sob SPD. O delineamento experimental utilizado foi o de blocos casualizados, com quatro repetições, em esquema fatorial 2 x 5. Constatou-se que a B. ruziziensis proporcionou maior competição com o milho em consórcio quando comparada à B. brizantha, causando redução dos teores de N e S foliares e no comprimento da espiga e massa de 100 grãos do milho. Porém, esta competição não interferiu na produtividade de grãos da cultura do milho o que também ocorreu com relação às doses de N aplicadas em cobertura. A B. brizantha produziu maior quantidade de massa seca comparada à B. ruziziensis, o que ocorreu também de modo ... (Resumo completo, clicar acesso eletrônico abaixo) / Abstract: As an alternative to increasing the productivity of production systems, the use of Crop- Livestock Integration (CLI) is increasing. Thus, it was evaluated in an Oxisol: 1) the nutritional content and readings of ICF in corn leaves, and yield components and grain yield of the crop in a consortium with two species of Brachiaria (B. brizantha . MG-5 and B. ruziziensis) submitted to sidedress N rates, 2) to evaluate the productivity of forage biomass after harvest of corn, also submitted to N, as well as the nutritional content, chemical composition and the rate decomposition of the straw of Brachiaria, 3) to assess the nutritional content and readings of ICF of common bean leaves of winter crop in rotation, as well as yield components and grain yield, and 4) to evaluate the economic performance of the CLI system, considering intercropped corn/grassland and common bean in succession. For this, three experiments were conducted in sequence, during the 2008/2009 agricultural year at the Experimental Station of Engineering Faculty - UNESP, Ilha Solteira Campus, in irrigated conditions in the Cerrado, with a history of eight years under no till. The experimental design was a randomized complete block with four replications in a 2 x 5 scheme. It was found that B. ruziziensis provided greater competition with corn intercropping compared to B. brizantha, causing reduction in N and S leaf, ear length and weight of 100 grains of corn. However, this competition did not affect the grain yield of corn, which also occurred with respect to N rates applied in bands. B. brizantha produced a greater amount of dry mass compared to B. ruziziensis, which also occurred in order linear with increasing doses of N topdressing for both species. Moreover, B. ruziziensis showed better chemical composition and nutritional status, being more suitable for animal nutrition or nutrient supply to crops in succession. With ... (Complete abstract click electronic access below) / Mestre

Orientador: Marco Eustaquio de Sá / Banca: Orivaldo Arf / Banca: Salatiér Buzetti / Banca: Rogério Peres Soratto / Banca: Charline Zaratin Alves / Resumo: A maioria dos trabalhos com diferentes sistemas de manejo do solo e com uso de regulador vegetal (citocinina, giberelina e auxina) busca obter resultados quanto a aumentos na produtividade. Já, quanto ao seu efeito nos teores de nutrientes nas folhas e sementes e na qualidade fisiológica das sementes produzidas nessas condições há carência de informações. Desta forma, o objetivo deste trabalho foi avaliar o desenvolvimento, produtividade, nutrientes nas folhas e sementes e qualidade fisiológica das sementes de feijão, cultivares Carioca Precoce, IAC Apuã e IAC Alvorada, em função do uso de regulador vegetal, via foliar, em sistema convencional e plantio direto em implantação, no inverno com uso de irrigação. A pesquisa foi conduzida em 2009 e 2010, no Município de Selvíria/MS. Realizaram-se três experimentos com os cultivares Carioca Precoce, IAC Apuã e IAC Alvorada, em blocos casualizados, em faixas, com quatro repetições. Os fatores foram: sistemas de semeadura convencional com incorporação da palhada do milheto ao solo e direta sobre a palhada do milheto e cinco doses do regulador vegetal (0; 1,0; 2,0; 3,0 e 4,0 L p.c ha-1) composto por três hormônios vegetais: cinetina, ácido giberélico e ácido indolbutírico, aplicadas em R5. Avaliou-se: clorofila e nutrientes foliares, características agronômicas e produtivas, teor de proteína e nutrientes nas sementes e a qualidade fisiológica das sementes através dos testes de: germinação, primeira contagem, emergência de plântulas em campo, índice de velocidade de emergência, altura de plântula, massa da matéria seca de plântula, envelhecimento acelerado, teste de frio sem solo e condutividade elétrica. O plantio direto mesmo em fase de implantação propiciou: aumento na clorofila e nos teores foliar de N, P e Mg variando em função do cultivar e do ano; e de maneira geral, considerando os três cultivares... (Resumo completo, clicar acesso eletrônico abaixo) / Abstract: The most studies with different systems of soil management and use of plant growth regulator (cytokinin, auxin and gibberellin) search results as increases in productivity. Already, in its effect on nutrient concentrations in leaves and seeds and physiological quality of seed produced under these conditions there is a lack of information. Thus, the objective was to evaluate the development, productivity, nutrients in the leaf and seeds and physiological seed quality of common bean cultivars, Carioca Precoce, IAC Apuã and IAC Alvorada, depending on the use of growth regulators on leaf, in the conventional or no-tillage system in the implantation, in the winter with irrigation use. The research was conducted in 2009 and 2010, in Selvíria - MS State. They were conducted three experiments with varieties Carioca Precoce, IAC Apuã and IAC Alvorada, in a randomized blocks design in strips with four replicates. The treatments were: conventional tillage system with millet straw incorporation to the soil or directly over the millet straw, and five doses of the plant growth regulators (0, 1.0, 2.0, 3.0 and 4.0 L cp ha -1) which consists of three plant hormones, kinetin, gibberellic acid and IBA, applied at R5. It was evaluated: chlorophyll and foliar nutrients, agronomic and productive characteristics, protein and nutrients in the seeds and physiological quality of seeds through the tests of germination, first count, seedling emergence in the field, speed of emergence index, seedling height, mass of dry matter of seedlings, accelerated aging, cold test without soil and electric conductivity. The no-tillage even in implantation phase resulted in: increased leaf chlorophyll and on N, P and Mg, depending on the cultivar and year, and in general, considering the three cultivars used, it was the better development of plants and seed yield. The growth regulator did not affect the levels... (Complete abstract click electronic access below) / Doutor

The common bean (Phaseolus vulgaris L.) is an important crop grown widely in Uganda. It is also an important source of income for smallholder farmers particularly women. Despite its importance, production in the cool highland regions is constrained by anthracnose disease which causes losses in both the quantity and the quality of beans produced. The principal aim of this research was to elucidate on the status of dry bean anthracnose and the genetics governing its resistance. A participatory rural appraisal study was conducted to explore farmers' knowledge, experience, problems and cultivar preferences in association with managing dry bean anthracnose disease. This study revealed that anthracnose is an important constraint to production which is not controlled in any way. Although farmers have varying cultivar preferences, they use mostly home saved seed and only 1% could access improved seed. The study suggested the need for practical approaches in the provision of quality anthracnose resistant seed in consideration of farmers' preferences and the dynamics of their rural livelihoods. A study was conducted to determine the variability of the anthracnose (Colletotrichum lindemuthianum) pathogen in some of the major bean growing regions of Uganda. Use was made of a set of 12 internationally accepted anthracnose differential cultivars to identify the physiological races present. The results obtained indicated the presence of eight races with one race (767) being dominant and most aggressive. Differential cultivars AB 136 and G2333 were resistant to all the eight races, and can be utilised as potential sources of resistant genes. A germplasm collection of mostly Ugandan accessions was screened for anthracnose resistance. Using the area under disease progression curve as the tool for assessing disease severity, eleven accessions were identified that posses good levels of anthracnose resistance. The yield loss attributed to the anthracnose disease was determined on three susceptible Ugandan market-class dry bean cultivars and two resistant cultivars. The results showed that the yield of susceptible cultivars was reduced by about 40% and an almost equivalent yield was lost due to poor quality seed. In comparison, the yield lost by the resistant cultivars was not significant. The study suggested the use of resistant cultivars as the best solution in combating anthracnose resistance. Three susceptible Ugandan market class dry bean cultivars and six resistant cultivars were used for the study of the inheritance of resistance to the anthracnose pathotype 767 in a complete 9x9 diallel design. The results clearly indicated that the resistance was predominately conditioned by additive gene action. It was also established that epistatic gene action was important. More than one pair of genes displaying partial dominance were responsible for determining resistance and the maternal effect did not have an influence on resistance. Additionally, the result showed that some of susceptible cultivars combined very well with the resistant cultivars and that anthracnose resistance heritability estimates in both the narrow and broad sense were high. These results suggested that the use of simple pedigree breeding procedures such as backcross selection could be useful in improving anthracnose resistance levels in the Ugandan market class varieties. / Thesis (Ph.D.)-University of KwaZulu-Natal, Pietermaritzburg, 2006.

Bean fly (Ophiomyia spp.) is a major pest of common bean (Phaseolus vulgaris L.) throughout eastern and southern Africa. In the semi-arid areas, apart from drought, the insect pest is reported to cause high crop losses up to 100%, particularly when drought occurs and under low soil fertility. Host-plant resistance is part of the integrated pest management strategies that have been widely employed against major insect pests of tropical legumes. However, information regarding its use in control of bean fly in common bean is limited. Therefore, the objectives of this study were to: (1) validate farmers’ perceptions of major constraints responsible for yield losses, particularly the major insect pests of beans; (2) asses the level of adoption of improved bean varieties and determine factors that influence farmers’ preferences of the varieties and criteria for selection; (3) identify sources of resistance to bean fly available in landraces; (4) determine the nature of gene action controlling bean fly resistance and seed yield in common bean; (5) describe a procedure for generating optimal bean fly populations for artificial cage screening for study of the mechanisms of resistance available in common bean against bean fly. Farmers considered drought and insect pest problems as main causes for low yields. The adoption rate for improved varieties was high but self-sufficiency in beans stood at 23% in the dry transitional (DT) agro-ecology and at 18% in the dry mid-altitude (DM) agroecology, respectively. Drought, earliness, yield stability, and insect pest resistance were the factors determining the choice of varieties by farmers. Bean fly (Ophiomyia spp.), African bollworm (Helicoverpa armigera) and bean aphid (Aphis fabae) were identified as key crop pests of beans limiting yield. The study to identify new sources of resistance included 64 genotypes consisting of landraces, bean fly resistant lines and local checks. The experiment was done under drought stressed (DS) and non-stressed (NS) environments and two bean fly treatments (insecticide sprayed and natural infestation) for three cropping seasons between 2008 and 2009. Genotypes differed in their reaction to natural bean fly attack under drought stressed (DS) and non-stressed environments (NS) over different cropping seasons. However, the effect of bean fly appeared to vary between the long rains (LR) and short rains (SR). It was observed that an increase in the number of pupae per stem resulted in a higher plant mortality. The range of seed yield was from 345 to1704 kg ha-1 under natural infestation and from 591 to 2659 kg ha-1 under insecticide protection. Seed yield loss ranged from 3 to 69 %. The resistance of most of the bean fly resistant lines seemed to be ineffective in presence of DS. To determine the nature of gene action controlling the inheritance of resistance to bean fly, four parents with known reaction to bean fly were crossed with four locally adapted genotypes in an 8 x 8 half-diallel mating design. Similarly, two resistant and two susceptible parents were selected and crossed to produce populations for generations means and variance components analysis. Results revealed that both general combining ability (GCA) and specific combining ability (SCA) mean squares were significant (p A 0.05) for all four traits studied, except SCA for stem damage during one cropping season. Among the parents, GBK 047858 was the best general combiner for all the traits studied across seasons except for stem damage during LR 2009. Genotypes GBK 047821 and Kat x 69 (a locally adapted variety) were generally good general combiners for resistance traits as well as seed yield. General predictability ratio values ranging from 0.63 to 0.90 were obtained for plant mortality, stem damage, pupae in stem and seed yield across cropping seasons. These results established the predominance of additive gene effects (fixable variation) over the non-additive effects in controlling the traits. Low to moderate narrow sense heritability values ranging from 0.22 to 0.45 were obtained for pupae in stem. Such heritability estimates indicate that although additive gene components were critical in the inheritance of resistance for the trait, non-additive gene action was also important in addition to the environmental effects. A major disadvantage in screening for resistance to bean fly in common bean by controlled means in net cages has been the lack of a method to use for raising adequate fly populations for screening. Due to this problem, a simple procedure for raising sufficient numbers of adult bean flies required for screening was described. Through this method, up to 62 % emergence of the adult flies was achieved. Moreover, the flies retained their ability to infest bean plants. To determine the presence of antibiosis and antixenosis mechanisms of resistance in common bean, five genotypes [CC 888 (G15430), GBK 047821, GBK 047858, Ikinimba and Macho (G22501)] and two local check varieties (Kat B1 and Kat B9) were screened under free-choice in outdoor net cages and no-choice conditions in net cages placed in a shadehouse. All the five resistant genotypes tested had relatively long internodes. It was established that long internode was a morphological trait associated with reduced pupation rate in bean stems, hence an antixenosis component of resistance. Both ovipositional non-preference and antibiosis mechanisms were found to exist in three genotypes namely CC 888 (G45430), GBK 047858 and Macho (G22501). These genotypes were resistant when they were subjected to bean fly under both free-choice and no-choice conditions. They had fewer feeding/oviposition punctures, low number of pupae in the stem, reduced damage to the stems and low percent plant mortality. The remaining genotypes, Ikinimba and GBK 047821 only expressed antixenosis. To maximize the effectiveness of host-plant resistance against bean fly, multiple insect resistances should be incorporated into a single bean genotype in order to ensure durability. However, this should be within the background of integrated pest management strategy. / Thesis (Ph.D.)-University of KwaZulu-Natal, Pietermaritzburg, 2010.

Dry bean (Phaseolus vulgaris L.) is economically and nutritionally an important legume, not only in Malawi, but in many parts of Africa and Latin America. Unfortunately, two bruchid species (Acanthoscelides obtectus Say, and Zabrotes subfasciatus Boheman) are known to cause extensive damage in storage, reducing the economic importance, food value and planting value of the crop. The aim of this study was to: i) ascertain farmers’ perceptions of the importance of bruchids as storage pests, and to identify their preferred varietal traits in dry beans; ii) screen Malawian dry bean landraces for effective and adaptable sources of resistance to the two bruchid species; iii) determine the gene action and inheritance of bruchid resistance. Farmers’ perceptions on the importance of the two bruchid species to beans both in the field and in storage were established using a participatory rural appraisal (PRA) in three extension planning areas (EPAs) in Lilongwe agricultural development division (ADD). Results confirmed that the two bruchid species are important storage pests, causing serious storage losses among smallholder farmers. In the absence of any control measures, farmers indicated that more than 50% of their stored beans could be lost to bruchids. Indigenous bruchid control measures are not very effective, making it necessary to search for other control methods. It was also clear from the PRA results that breeders need to consider both agronomic and culinary traits in bean cultivar development. This would enhance uptake of newly developed varieties. To address the problem of bruchid damage experienced by smallholder farmers, a total of 135 dry bean genotypes, comprising 77 landraces and 58 improved varieties (obtained from collaborating partners) were tested under laboratory infestation (nochoice test methods) and field infestation (free-choice test methods). The objective of this study was to identify effective sources of resistance to the two bruchid species. Results of the study showed that there was a wide variation among the genotypes for resistance to the two bruchid species. Overall results showed that 88% of the genotypes ranged from susceptible to highly susceptible to Z. subfasciatus and only 12% of the genotypes were moderately resistant to resistant. Genotype screening for resistance to A. obtectus showed that only 12.5% were resistant, whereas 87.5% were moderately to highly susceptible. All of the improved genotypes were 100% susceptible to A. obtectus in storage. One landrace, KK35, consistently showed a high level of resistance to both bruchids under laboratory infestation, with results similar to the resistant checks (SMARC 2 and SMARC 4), while another landrace, KK90, displayed stable resistance under both laboratory and field infestation. However, performance of most genotypes was not consistent with field and laboratory screenings, suggesting that mechanisms of bruchid resistance in the field are different from that in the laboratory and field screening should always be used to validate laboratory screening. Resistance in the field was not influenced by morphological traits. The seed coat played a significant role in conferring resistance to both bruchid species in the laboratory, whereas arcelin did not play any significant role in conferring resistance in the landraces. The inheritance of resistance to A. obtectus was studied in a 6 x 6 complete diallel mating design, involving crosses of selected Malawian dry bean landraces. The F1 crosses, their reciprocals, and six parents were infested with seven F1 generation (1 to 3 d old) insects of A. obtectus in a laboratory, no-choice test. There were significant differences among genotypes for general combining ability (GCA) and specific combining ability (SCA). However, SCA accounted for 81% of the sum of squares for the crosses, indicating predominance of the non-additive gene action contributing to bruchid resistance. A chi-square test for a single gene model showed that 5 of the 13 F2 populations fitted the 1:2:1 segregation ratio of resistant, intermediate and susceptible classes, respectively indicating partial dominance. The eight F2 populations did not conform to the two gene model of 1:4:6:4:1 segregation ratio of resistant, moderately resistant, moderately susceptible, susceptible and highly susceptible classes, respectively. Average degree of dominance was in the partial dominance range in five F3 populations, but in general resistance was controlled by over-dominance gene action in the F2 populations. The additive-dominance model was adequate to explain the variation among genotypes indicating that epistatic effects were not important in controlling the bruchid resistance. The frequency distribution of the 13 F3 populations for resistance to A. obtectus provided evidence for transgressive segregation, suggesting that resistance is conditioned by more than one gene. Reciprocal differences were not significant in the F2 generation seed; but were significant in four crosses in the F3 generation seed for adult bruchid emergence, suggesting that maternal effects or cytoplasmic gene effects also played a role in the inheritance of resistance to the common bean weevil. Through this study, important sources of bruchid resistance in dry bean have been identified in Malawian landraces (KK35, KK90 and KK73). These resistant sources will be used in a breeding programme to develop bruchid resistant bean cultivars, as well as improve resistance in susceptible commercial bean cultivars currently grown by farmers in Malawi. / Thesis (Ph.D.)-University of KwaZulu-Natal, Pietermaritzburg, 2007.

Wild mustard (Brassica spp.) is used as an edible wild leafy vegetable by indigenous people in South Africa. The potential of wild leafy vegetables in agriculture is not well understood, because there is generally no agronomic research on their production practices. The objective of this study was to examine the performance of three wild mustard species (herein referred to as I, K and M) over four cropping seasons in an intercropping system with green beans (Phaseolus vulgaris L. cv. Imbali). The crops were grown with and without organic fertiliser under dryland conditions at two sites (The University of KwaZulu-Natal Research Farm, Ukulinga and in a rural area of Umbumbulu, KwaZulu-Natal within the farmers' locality) during autumn, winter, spring and summer of 2004 to 2005. Plant development (leaf number, plant height and fresh biomass) during the first six weeks after sowing and seed yield were used to determine agronomic performance of each species. Nutrient status of the rhizosphere soil was determined at 42 days after sowing for each species to determine what effect growing the species would have on mineral availability. Wild mustard production significantly (P < 0.01) performed better at Ukulinga than Umbumbulu. Polyculture was beneficial for wild mustard leaf accumulation and green bean production as determined by land equivalent ratios greater than one for all species combinations, regardless of fertiliser application. Cool environmental conditions occurring in autumn and spring were more favourable (P < 0.05) for wild mustard and green bean biomass accumulation than summer and winter conditions. However, wild mustard seed yield was highest in winter compared with autumn and spring, and there was no measurable seed production in summer. Soil analysis results at 42 days after sowing showed an increase in P, K, Cu and Mg in the rhizosphere of wild mustard without organic fertiliser. Polyculture improved Zn, Cu, Mn and K in wild mustard leaf tissue. It is concluded that wild mustard can be grown as a leafy vegetable throughout the year, but it requires cool environmental conditions to enhance seed yield. Species M significantly yielded better biomass and seeds than species I and K during all the seasons. However, species K performed the least in all aspects. / Thesis (M.Sc.)-University of KwaZulu-Natal, Pietermaritzburg, 2005.

Submitted by Johnny Rodrigues (johnnyrodrigues@ufcg.edu.br) on 2018-07-01T14:30:28Z No. of bitstreams: 1 JOSÉ RAMOS DE QUEIRÓZ - DISSERTAÇÃO PPGEA 2011..pdf: 10586067 bytes, checksum: 59de6a3167d2dba506f41cae2f3984bb (MD5) / Made available in DSpace on 2018-07-01T14:30:28Z (GMT). No. of bitstreams: 1 JOSÉ RAMOS DE QUEIRÓZ - DISSERTAÇÃO PPGEA 2011..pdf: 10586067 bytes, checksum: 59de6a3167d2dba506f41cae2f3984bb (MD5) Previous issue date: 2011-09 / O objetivo deste trabalho foi desenvolver um equipamento que simule os danos mecânicos nas diversas etapas do processo de beneficiamento e avalie em cada etapa, as alterações da qualidade fisiológica (germinação e vigor, massa da matéria seca) das sementes de feijão carioca e também as alterações de suas características físicas (pureza física, massa de milsementes, massa específica aparente, porosidade e danos mecânicos). Inicialmente, fez-se um teste em uma UBS (Unidade de Beneficiamento de Sementes) através do qual foram levantados os parâmetros: distância, velocidade e tempo de duração do processo de beneficiamento na UBS para viabilizar a construção do simulador. As etapas estudadas foram recepção, prelimpeza, secagem nas temperaturas de 25, 35 e 45°C, limpeza, seleção e tratamento fúngico/ensaque. Concluiu-se, neste trabalho, que a qualidade fisiológica das sementes de feijão não é alterada significativamente da etapa de recepção até o momento anterior à secagem. Após a secagem a semente de feijão sofre perda significativa de sua qualidade fisiológica, embora não seja até a etapa final de beneficiamento. A perda de germinação e o vigor foram em média de 11,55 e 23,78 pontos percentuais, respectivamente; no entanto, as sementes apresentam no final do processo poder germinativo acima do mínimo indicado para sua comercialização (86 a 82,67%). / The objective of this project was to develop equipment that simulates the mechanical damages in various beneficiai processing steps and evaluates in each step the physiological quality changes (germination and vigor) of the seeds of carioca beans as well as the changes of their physical characteristics (purity fisic, mass of one thousand seeds, apparent specific mass, porosity and mechanical damages). First, a test was done in one SPU (Seeds Processing Unit) through which parameters were inventoried: distance, speed and time processing duration in the SPU to make possible the construction of the Simulator. The steps studied were pre- cleanliness, drying under temperatures of 25, 35 and 45°C cleanliness, selection and treatment of fungus and bagging. One concluded that the physiological quality of the bean seeds is not significant from the time when it gets to the SPU until prior to the time of drying. After drying the bean seed suffers important loss of its physiologic quality, although does not change up to the final beneficiai process step. The loss of germination and vigor carne at a rate of 11.55% and 23.78% respectively; but the seeds presented germination strength above the minimum indicated to commercialize them (86% to 82.67%).

Conselho Nacional de Desenvolvimento Científico e Tecnológico / The study aimed to test the efficiency of the method Papadakis on increasing the quality of experiments with snap beans, zucchini, peppers and lettuce, and having efficiency, determine how to estimate the covariate and plot size for use in experiments where apply the method. The definition of the form of estimated covariate that provides greater efficiency and calculation method Papadakis plot size adjusted for use in this method were made from the following blank experiments: two experiments with zucchini, lettuce with eight, five-bean pod and two peppers. In experiments with zucchini, pole beans and peppers, the variable was the weight of fruits and lettuce in experiments with variable mass was fresh shoot. The effectiveness of the use of analysis of covariance (ANCOVA) with the covariate estimated by Papadakis was tested from treatment experiments: one with the bell pepper crop, where the variable was the weight of fruit and one with lettuce crop where the variable was the fresh weight of shoots. The use of ANCOVA with the covariate estimated by Papadakis increases the quality of experiments with vegetable crops. The covariate that provides the highest efficiency ANCOVA is that which is determined considering a portion neighboring each side of the reference portion towards the row of plants. The plot size in the crop row, adjusted for method, is 10 plants (2.0 m) for snap beans, five plants (4.5 m) to zucchini, five plants (1.5 m ) for chili, and four plants (1.2 m) for lettuce. / O trabalho teve por objetivo testar a eficiência do método Papadakis no aumento da qualidade de experimentos com feijão-vagem, abobrinha italiana, pimentão e alface e, havendo eficiência, determinar a forma de estimativa da covariável e o tamanho de parcela para uso em experimentos onde se aplicará o método. A definição da forma de estimativa da covariável que proporciona maior eficiência do método Papadakis e o cálculo do tamanho de parcela ajustado para uso no mesmo foram realizados a partir dos seguintes experimentos em branco: dois experimentos com abobrinha italiana, oito com alface, cinco com feijão-vagem e dois com pimentão. Nos experimentos com abobrinha italiana, feijão-vagem e pimentão, a variável foi o peso de frutos e nos experimentos com alface a variável foi massa fresca da parte aérea. A eficácia do uso da análise de covariância (ANCOVA) com a covariável estimada pelo método Papadakis foi testada a partir de experimentos com tratamentos: um com a cultura do pimentão, onde a variável foi o peso de frutos e, um com a cultura da alface, onde a variável foi a massa fresca da parte aérea. O uso de ANCOVA com a covariável estimada pelo método Papadakis aumenta a qualidade de experimentos com culturas olerícolas. A covariável que proporciona a maior eficiência da ANCOVA é aquela que é estimada considerando uma parcela vizinha de cada lado da parcela de referência no sentido da linha de cultivo. O tamanho de parcela na linha de cultivo, ajustado para uso do método, é de 10 plantas (2,0 m) para feijão-vagem, de cinco plantas (4,5 m) para abobrinha italiana, cinco plantas (1,5 m) para pimentão, e de quatro plantas (1,2 m) para alface.

Orientador: Orivaldo Arf / Banca: Salatiér Buzetti / Banca: Rogério Peres Soratto / Resumo: O nitrogênio é o nutriente absorvido em maior quantidade pelo feijoeiro, sendo que o manejo da adubação nitrogenada pode interferir no desenvolvimento e na produtividade da cultura. Neste sentido, o trabalho foi desenvolvido na Fazenda Experimental da UNESP - Ilha Solteira localizada em Selvíria, Mato Grosso do Sul, com o objetivo de estudar o efeito de fontes e épocas de aplicação do nitrogênio no desenvolvimento e produtividade do feijoeiro de inverno no sistema plantio direto utilizando irrigação por aspersão em dois anos de cultivo (2006 e 2007). O solo do local é do tipo Latossolo Vermelho argiloso, a área foi cultivada nos 5 anos anteriores com milho no verão e feijão no inverno. O cultivar utilizado foi o Pérola e o delineamento experimental foi o em blocos casualizados com 16 tratamentos dispostos em esquema fatorial 2 x 8, sendo duas fontes de nitrogênio (uréia e sulfonitrato de amônio), aplicadas em sete ocasiões (30 dias antes da semeadura, 15 dias antes da semeadura, na semeadura, 15 dias após a semeadura, 30 dias após a semeadura, 1/3 na semeadura + 2/3 15 dias após a semeadura, 1/3 na semeadura + 2/3 30 dias após a semeadura) na dose de 90 kg de N ha-1, além da testemunha (sem aplicação de N). Os resultados obtidos revelaram influência das fontes na população de plantas somente no segundo ano de cultivo e a população média de plantas em cada ano de cultivo pode ter influenciado direta ou indiretamente as demais variáveis. Maiores valores de massa seca de plantas foram observados quando o nitrogênio foi aplicado em dose total na semeadura. O teor de nitrogênio foliar não apresentou diferença entre os tratamentos devido à fontes ou épocas de aplicação de N, porém em 2006 os tratamentos que receberam N foram superiores à testemunha. O número de vagens planta-1 e de grãos planta-1 não foram influenciados...(Resumo completo, clicar acesso eletrônico abaixo) / Abstract: Nitrogen is the nutrient uptaken in larger amount in common bean, and the management of the nitrogen fertilization can interfere in common bean crop yield. The work was developed at experimental station of University of São Paulo State - Ilha Solteira - Campus, located in Selvíria, State of Mato Grosso do Sul - Brazil, on a dystrophic clayey Haplic Acrustox cultivated in the previous five years with corn in the summer and common bean in the winter. The objective was to study the effect of sources and time of N application in common bean growth cropped under no-tillage, irrigated by sprinkle. Pérola cultivar was used and the experimental design was a randomized complete blocks, with 16 treatments in a factorial scheme 2 x 8: two sources of nitrogen (urea and ammonium sulfonitrate - fertilizer with inhibitor of nitrification), applied in seven times (30 days before sowing, 15 days before sowing, at sowing, 15 days after sowing, 30 days after sowing, 1/3 at sowing + 2/3 15 days after sowing, 1/3 at sowing + 2/3 30 days after sowing) and the control (without N) in two cropping years (2006 and 2007). The obtained results only revealed influence of sources in the stand at the second cropping year and the medium stand obtained in each cropping year might have influenced direct or indirectly the other results. Higher values of plant dry mass were obtained when the nitrogen was applied in total dosage at sowing. The nitrogen content on the leaves did not present difference among treatments; however, in 2006 the treatments that received N were superior to the control. The number of pods per plant and seeds per plant were not influenced by treatments. The number of seeds per pod and the mass of 100 grains were influenced by times of nitrogen application in 2006; however, with apparent relation to other production components results. The sources and nitrogen time application did not influence the grain yield of winter common bean in no till system. / Mestre

The Neon-S method has been used for the detection of Sclerotinia sclerotiorum in soybean and common beans since the agricultural year 2010. However, this method yields false positive readings due to other fungi that change medium pH. Thus, this study evaluated the contribution of increasing incubation period on the optimization of Neon-S reliability in different cultivars of soybeans and common beans. Nine treatments were evaluated in randomized blocks design, as a 3x3 factorial, with three replications. The factors were three detection methods (paper roll, Neon- S, and Neon S2) and three cultivars: naturally infected Pérola beans; naturally infected soybean cultivar BRS Valiosa RR, and artificially inoculated soybean cultivar Nidera 7255 RR). Inoculation of soybean Nidera 7255 RR was done by incubating the seeds for 72 h on PDA where the fungus was previously grown for seven days at 25 ºC. The three methods were compared, evaluating 400 seeds per replication, after incubation for seven days in Neon-S, for 15 days in Neon-S2, and for 30 days in paper roll, determining the presence of the fungus, and that of sclerotia adhered to the seeds. Twenty seeds were used per plate for media Neon-S and Neon-S2 (20 plates per replication), and 50 seeds per roll for the paper roll. Seeds were incubated at 20 ºC in BDO chamber in darkness. The data were submitted to the analysis of variance and the averages compared by the Tukey test at 5% probability. The interaction between detection method and cultivar was significant at 1% probability, indicating that the best method depends on the cultivar under study. Among the cultivars evaluated, artificially infected soybean presented the greatest indices of pathogen infection. A comparison of seed lots analyzed at the Plant Disease Clinic LAMIP proved greater sensibility of Neon-S in a total of 637 lots evaluated from 2008 to 2012. In that period, the paper roll test resulted in 21.88% positive samples, while in Neon-S 31.25% of them were positive. Another distinguishing factor among the lots analyzed was the percentage of infected and, or, contaminated seeds, which varied from one (0.25%) to five (1.25%). Therefore, detection of S. sclerotiorum by the Neon-S2 method can be optimized by incubation for 15 days, considering that, in this case, the formation of sclerotia near the infected seeds confirm the presence of the pathogen, avoiding false positive readings. Neon-S2 method increased detection sensibility of S. sclerotiorum in seed lots analyzed in comparison with the paper roll method; however, in comparison with Neon S method, despite its greater average, no significant differences were observed. / O método de Neon-S tem sido utilizado para a detecção de Sclerotinia sclerotiorum em sementes de soja e feijão a partir da safra de 2010. Porém, esse método possibilita a leitura de falsos positivos devido ao aparecimento de fungos que também alteram o pH do meio. O objetivo deste trabalho foi verificar se o aumento do período de incubação pode contribuir para otimizar a confiabilidade do teste Neon-S em diferentes cultivares de soja e feijão. Foram avaliados nove tratamentos em delineamento de blocos casualizados no esquema fatorial 3x3, com três repetições, sendo três métodos de detecção: rolo de papel, Neon-S e Neon S2 e três cultivares: Feijão Pérola infectados naturalmente; soja cultivar BRS Valiosa RR infectadas naturalmente e soja cultivar Nidera 7255 RR inoculada artificialmente. Para a inoculação da soja Nidera 7255 RR, as sementes foram incubadas em meio de cultivo BDA por 72 h, após o cultivo do fungo por sete dias na temperatura de 25 ºC. Os três métodos foram comparados, avaliando 400 sementes por repetição, meio Neon-S com incubação de sete dias, Neon-S2 com incubação de quinze dias e o rolo de papel por trinta dias, anotando-se a presença do fungo e a presença de escleródios aderidos às sementes. No meio Neon-S e Neon-S2 foram utilizadas 20 sementes por placa (20 placas por repetição). No rolo de papel foram usadas 50 sementes por rolo. As sementes foram incubadas 20 ºC em BOD, no escuro. Realizou-se a análise de variância dos dados e teste de comparação de médias (Tukey 5%). A interação entre o método de detecção e a cultivar utilizada foi significativo a 1% de probabilidade, indicando que o melhor método depende da cultivar avaliada. Dentro das cultivares avaliadas, a soja infectada artificialmente foi a que apresentou os maiores índices de infecção pelo patógeno. Usando os resultados de lotes de sementes, comprovou-se a maior sensibilidade do Neon-S num montante de 637 lotes testados no período de 2008 a 2012. Durante esse período, o teste de rolo apresentou 21,88% de amostras positivas, enquanto que o Neon-S foi de 31,25%. Outro fator diferenciador em relação aos lotes analisados foi a porcentagem de sementes contaminadas e ou infectadas, que variou de uma (0,25%) a cinco (1,25%). Assim a detecção de S. sclerotiorum pelo método Neon-S2 pode ser otimizada com a incubação por 15 dias, considerando que, neste caso, a formação de escleródios próximos às sementes infectadas confirma a presença do patógeno e evita a leitura de falsos positivos. O método Neon-S2 aumentou a sensibilidade de detecção de S. sclerotiorum em lotes de sementes analisadas quando comparado ao método de rolo, porém, quando comparado com o método Neon S, sua média foi maior, mas a diferença não foi significativa. / Mestre em Agronomia