Orientador: Graziella Nuernberg Back Brito / Banca: Luciane Vieira Garcia / Banca: Fernanda Malagutti Tomé / Resumo: O objetivo do trabalho foi investigar se os extratos de Pfaffia paniculata K. e Juglans regia L. possuem ação antifúngica, antibacteriana e toxicidade celular, com testes in vitro. Para os testes antifúngicos foram utilizadas cepas ATCC de Candida spp., e para os testes antibacterianos foram utilizadas cepas ATCC de Enterococcus faecalis, Staphylococcus aureus, Staphylococcus epidermidis, Streptococcus mutans e Pseudomonas aeruginosa. Para a atividade antimicrobiana primeiramente foram determinados os valores da Concentração Inibitória Mínima (CIM) e da Concentração Microbicida Mínima (CMM) dos extratos pelo método de microdiluição em caldo, segundo Clinical and Laboratory Standards Institute (CLSI). Os micro-organismos que apresentaram CMM foram selecionados para os testes em biofilme, no qual foi preparado em fundo de placa com 96 poços, por 48 h. Após os biofilmes foram tratados por 5 min. utilizando as concentrações de 200, 100 e 50 mg dos extratos. Para mensuração da biomassa foi utilizado o teste de Cristal violeta (CV), e para avaliar a atividade metabólica foi utilizado o teste de MTT. A citotoxicidade foi avaliada sobre fibroblastos gengivais humanos (FMM-1) utilizando os mesmos parâmetros de tratamento utilizados para os testes em biofilmes. Foram avaliadas a viabilidade celular pelos testes de MTT, vermelho neutro e cristal violeta. Os dados obtiveram distribuição normal e foram analisados por ANOVA e teste de Tukey, com significância de 5% (p<0.05%). O extrato ... (Resumo completo, clicar acesso eletrônico abaixo) / Abstract: The aim of this study was to investigate whether extracts of Pfaffia paniculata K. and Juglans regia L. have antifungal, antibacterial and cellular toxicity, with in vitro tests. ATCC strains of Candida spp. Were used for antifungal tests, and ATCC strains of Enterococcus faecalis, Staphylococcus aureus, Staphylococcus epidermidis, Streptococcus mutans and Pseudomonas aeruginosa were used for the antibacterial tests. For the antimicrobial activity, the values of the Minimum Inhibitory Concentration (MIC) and the Minimal Microbicidal Concentration (CMM) of the extracts were determined by the microdilution method in broth, according to Clinical and Laboratory Standards Institute (CLSI). The microorganisms that presented CMM were selected for the biofilm tests, in which it was prepared on a 96-well plate bottom for 48 h. After the biofilms were treated for 5 min. Using the concentrations of 200, 100 and 50 mg of the extracts. To measure the biomass, the Violet Crystal test (CV) was used, and the MTT test was used to evaluate the metabolic activity. Cytotoxicity was assessed on human gingival fibroblasts (FMM-1) using the same treatment parameters used for biofilm tests. Cell viability was evaluated by the MTT, neutral red and violet crystal tests. The data obtained normal distribution and were analyzed by ANOVA and Tukey test, with significance of 5%. The extract of P. paniculata showed antifungal action in biofilms, with average reductions of 29.4 and 42.7% in CV and MTT tests; The antibacterial action was restricted to S. mutans and P. aeruginosa with mean reductions of 15.7 and 28.6% in the respective tests. The extract of J. regia also demonstrated antifungal action with an average reduction of 22.2% in biomass and 31.4% in metabolic activity. The antimicrobial action was restricted to P. aeruginosa with mean reductions of 17.7 and 15.6%,....(Resumo completo, clicar acesso eletrônico abaixo) / Mestre

Thesis - University of Pennsylvania. / Error in pagination: p. 16 is misnumbered 15. Film 633 reel 76 is part of Research Publications Early American Medical Imprints collection (RP reel 76, no. 1564). DNLM Includes bibliographical references.

Thesis (doctoral)--Universität, Regensburg, 2006.

Orientador: Ana Carolina de Souza Chagas / Banca: Márcia Cristina de Sena Oliveira / Banca: Gilson Pereira de Oliveira / Resumo: O parasitismo por Rhipicephalus (Boophilus) microplus ocasiona grandes perdas econômicas nos rebanhos bovinos, e seu controle por meio de carrapaticidas sintéticos enfrenta problemas de resistência, além do risco de resíduos nos alimentos e ambiente. Com o objetivo de contribuir com informações relevantes para o controle desse parasita, o presente estudo teve três objetivos: 1) avaliar in vitro o efeito do óleo essencial de Ocotea elegans "canela-sassafrás" sobre larvas e fêmeas ingurgitadas de R. (B.) microplus; 2) avaliar medicamento homeopático no controle de infestações naturais; 3) comparar as técnicas de estimativa visual e de quantificação por amostragem de larvas eclodidas no teste de imersão de fêmeas adultas, visto a importância dessas no levantamento de substâncias com ação carrapaticida. No primeiro experimento "Efeito acaricida e repelente in vitro do óleo essencial de Ocotea elegans (canela-sassafrás) sobre Rhipicephalus (Boophilus) microplus", foram adotados os testes: contato de larvas em papel impregnado (LPI), teste de repelência larvar (TR) e imersão de fêmeas adultas (AIT). A fim de verificar a eficácia, O. elegans foi avaliada nas concentrações de 0,78 mg/mL a 100 mg/mL. No AIT, a partir da concentração de 25 mg/mL, a eficácia foi superior a 90%. Os LPI (48h) a 100 mg/mL resultaram em taxas de mortalidade acima de 70%. Já o TR obteve repelência superior a 80% a partir da menor concentração avaliada. Em relação às concentrações letais (CL), a menor CL50 ob... (Resumo completo, clicar acesso eletrônico abaixo) / Abstract: The parasitism by Rhipicephalus (Boophilus) microplus causes large economic loss in cattle herds and its control through chemical acaricides faces problems of resistance and residues in food and in the environment. Aiming to contribute with relevant information for the control of this parasite, the present study had three objectives: 1) evaluate the effect in vitro of Ocotea elegans essential oil on larvae and engorged females of R. (B.) microplus, 2) evaluate homeopathic medicine to control R. (B.) microplus natural infestations, and 3) compare visual estimation and quantification by sampling techniques of larvae hatching in the engorged females immersion test, due to the importance of this technique in the screening of substances with antiparasitic action. In the first experiment, "In vitro acaricidal and repellent effect of Ocotea elegans essential oil on R. (B.) microplus", were adopted the tests: larval packet test (LPT), the larval repellency test (RT) and the adult immersion test (AIT). In order to verify efficacy, O. elegans was evaluated at concentrations of 0.78 mg/mL to 100 mg/mL. In the AIT, from the concentration of 25 mg/ml, the efficacies were higher than 90%. LPT (48h) at 100 mg/mL resulted in mortality rates higher than 70%. The RT obtained repellency higher than 80% sice the lowest concentration evaluated. In relation to the lethal concentrations, the lowest LC50 obtained in the LPT was 25.59 mg/mL, 48 hours post treatment. The LC50 and LC90 in the AIT were 4.96 and 17.37 mg/mL and in TR were 0.04 and 1.24 mg/mL, respectively. It was possible to conclude that O. elegans had significant effect in engorged females and larvae repellency and may be in the future an adjuvant for tick control, prioritizing in vivo tests. In the second experiment, "Study of homeopathy in the control of natural infestation of R. (B.) microplus in dairy cattle... (Complete abstract click electronic access below) / Mestre

Orientadores: Jose Francisco Hofling, Marta Cristina Duarte Teixeira / Dissertação (mestrado) - Universidade Estadual de Campinas, Faculdade de Odontologia de Piracicaba / Made available in DSpace on 2018-08-09T04:57:27Z (GMT). No. of bitstreams: 1 Obando-Pereda_GustavoAlberto_M.pdf: 3782174 bytes, checksum: d7ee038a5b8df1578af994efc4637544 (MD5) Previous issue date: 2007 / Resumo: Tem-se demonstrado que espécies de Candida podem também ser encontradas no biofilme oral, co-agregadas ou aderidas a espécies bacterianas ali presentes, preferencialmente à espécies de Estreptococos, como também há adesão destas leveduras às células do epitélio e aos aparatos protéticos (neste caso é muito comum que pacientes portadores de próteses sejam alvos para as infecções fúngicas). Assim, a habilidade das espécies de Candida para formar biofilme em dispositivos médicos tem ampliado a capacidade de causar doenças assim como a capacidade de resistir a antifúngicos. Por outro lado nas ultimas décadas, tem se observado um crescente interesse nas medicinas alternativas e nas terapias naturais, justificando o aumento significativo de pesquisas nessa área ampliandose no presente. O objetivo desta pesquisa é, primeiramente, avaliar a interação das espécies de Candida albicans, C. tropicalis e C. glabrata em biofilmes préformados por espécies de Streptococcus oralis, S. sanguis, S. mitis, S. mutans e Staphylococcus aureus em diferentes materiais protéticos como: titânio e resina acrílica, e, secundariamente, testar a ação de alguns extratos fitoterápicos como a Mentha piperita, Cymbopogum martinii e Cympopogum winterianus através da concentração mínima inibitória (CMI), na inibição da co-agregação das espécies de Candida spp. a estes microrganismos. Os dados obtidos nesta pesquisa demonstram que as espécies de Candida albicans desenvolvem biofilme sobre os biofilmes pré-formados das bactérias testadas independentemente do material avaliado. A presença de bactérias se demonstra determinante para o desenvolvimento de biofilme por espécies de Candida. A inibição de Candida spp. pelos extratos vegetais testados se mostrou parcial e semelhante quando comparadas, ao lado de se revelarem substâncias potencialmente antifúngicas / Abstract: Candida spp. have been demonstrated found in the oral biofilm, coaggregated or adhered to oral bacteria, especially Streptococcus spp., also too the adhesion of these yeast to mucosal cells and prosthetic devices (in this case is common that patients that carry a prosthetic device, could be targets to fungal infections). The ability of Candida spp. to form biofilm on medical devices has extended the capacity to cause diseases and to resist antifungal agents. The lasts decades, have been observed big interest concern to medicinal plants and natural therapies, justifying the significant increase of research on the area in the present. The aim of this research was firstly, evaluate the interaction of C. albicans, C. tropicalis and C. glabrata on S. oralis, S. sanguis, S. mitis, S. mutans and Staphylococcus aureus preformed biofilm in different prosthetics materials like titanium and acrylic resin. Secondly, was evaluated the action of some vegetal extracts like Mentha piperita, Cymbopogum martinii and Cympopogum winterianus, using the Minimal Inhibitory Concentration (MIC), against the Candida spp. coaggregation with these microorganisms. The data obtained in this research show that Candida spp. develop biofilm on preformed bacterial biofilm, independently of tested material. Bacterias are determinant to Candida spp. biofilm development. The Candida spp. inhibition for vegetal extract was partial and equal when compared, revealing itself like potentially antifungal substances / Mestrado / Microbiologia e Imunologia / Mestre em Biologia Buco-Dental

Malgré l’arsenal antifongique existant aujourd’hui, les mycoses superficielles sont en constante progression de par le monde et notamment dans le bassin caribéen. Nous nous sommes focalisés sur les pathologies qui posent, en Guadeloupe, de nombreux problèmes de résistance ou de rechute vis-à-vis des antifongiques actuels, à savoir : les dermatophyties, le Pityriasis versicolor (Malassezia sp.), les candidoses et les scytalidioses. Suite à ce constat, nous nous sommes tournés vers la flore guadeloupéenne où ont été sélectionnées dix plantes sur des critères ethnobotaniques, chimiotaxonomiques ou encore d’observations naturalistes avec un double objectif : trouver de nouveaux extraits antifongiques d’une part et, d’autre part, étudier leur composition et vérifier leur innocuité. Le screening antifongique in vitro des extraits c-hexane, EtOH et EtOH/eau (1:1, v/v) a été réalisé vis-à-vis de 4 dermatophytes, 1 Malassezia sp., 5 Candida spp. et 1 Scytalidium sp. Les extraits ont également été testés vis-à-vis d’un autre pathogène, Pneumocystis jirovecii responsable de la pneumocystose pulmonaire. Quatre plantes : Bursera simaruba, Cedrela odorata, Enterolobium cyclocarpum et Pluchea carolinensis ont été retenues afin de définir leurs cytotoxicités puis de procéder à l’isolement des composés responsables de leur activité antifongique par bioguidage. Cedrela odorata a montré une activité significative vis-à-vis de Pneumocystis jirovecii due en partie à la (+)-catéchine. Concernant les mycoses superficielles, Bursera simaruba et Cedrela odorata présentent une activité due à une synergie de composés non identifiés par bioguidage alors que Pluchea carolinensis et Enterolobium cyclocarpum doivent respectivement leurs activités à des flavonoïdes sulfatés et à des saponosides triterpéniques. Faisant suite à cette étude phytochimique et pharmacologique, la formulation des extraits sous forme de gels et vernis a été développée. Ainsi, cette étude permet d’apporter une réponse originale et efficace aux pathologies ciblées. / Despite the existing arsenal of antifungals today, superficial fungal infections have increased over the world and especially in the Caribbean basin. We focused our work on diseases that pose, in Guadeloupe, many problems of resistance or recurence towards current antifungals : dermatophytosis, Pityriasis versicolor (Malassezia sp.), Candidosis and Scytalidiosis. Following this observation, we were interested in the flora of Guadeloupe where ten plants were selected on natural observation, ethnobotanical or chemotaxonomical criteria with a dual purpose: to find new antifungal extracts on the one hand, and secondly, to study their composition and verify their safety. The in vitro screening of c-hexane, EtOH and EtOH/water (1:1, v/v) extracts, was made towards four dermatophytes, one Malassezia sp., five Candida spp. and one Scytalidium sp. The extracts were also tested on another pathogen, Pneumocystis jirovecii which is responsible of pneumonia. Four plants: Bursera simaruba, Cedrela odorata, Enterolobium cyclocarpum and Pluchea carolinensis were chosen to define their cytotoxicities and then we proceed to the isolation of active compounds by bioguiding. Cedrela odorata showed significant activity on Pneumocystis jirovecii and (+)-catechin was found to be partly responsible of this activity. Concerning the research on the superficial mycoses, Bursera simaruba and Cedrela odorata’s activities were due to a synergy of compounds unidentified by bioguiding while Pluchea carolinensis and Enterolobium cyclocarpum owe their activities to sulfated flavonoids and triterpene saponins, respectively. Following to these phytochemical and pharmacological studies, the drug formulation of the extracts has been developed. Thus, this study could be an original and effective response to the targeted disease.

Thesis (Dr. of Physic)--Columbia College, 1802. / Signatures: [A]⁴ B-C⁴. Film 633 reel 16 is part of Research Publications Early American Medical Imprints collection (RP reel 16, no. 279). DNLM

O presente estudo teve como objetivo avaliar e comparar, morfologicamente, a resposta do tecido subcutâneo de ratos frente ao de hidróxido de cálcio associado a diferentes substâncias, sendo elas: clorexidina 0,2% em propilenoglicol, clorexidina 0,4% em propilenoglicol, Casearia sylvestris Sw (Guaçatonga) em propilenoglicol e propilenoglicol (que serviu como grupo controle). Para isso, foram utilizados 72 ratos Wistar. As pastas foram preparadas e colocadas em tubos de polietileno, que foram implantados no tecido subcutâneo do dorso de cada animal. Os períodos experimentais foram de 7, 14 e 30 dias após o procedimento cirúrgico, quando então, os animais foram mortos. As amostras coletadas passaram por processamento histotécnico e foram feitos cortes de 5µm de espessura e coloração com Hematoxilina e Eosina (H.E.). A avaliação das respostas do tecido conjuntivo em contato com as pastas testadas foirealizada de forma descritiva e quantitativa subjetiva.Os dados obtidos após análise quantitativa foram submetidos ao teste estatístico de Kruskal-Wallis e Dunn com nível de significância p<0,05. Os resultados mostraram que a clorexidina 0,4%, de modo geral, se mostrou mais irritante que os demais grupos. O extrato de Casearia sylvestris Sw apresentou comportamento satisfatório em relação à intensidade da reação inflamatória, igualando-se aos grupos 1 e 4. Com base na metodologia aplicada, foi possível concluir que aos 30 dias todos os grupos mostraram não interferir no processo de reparo e que todas as substâncias experimentais (clorexidina a 0,4%, a 0,2% e o extrato de Casearia sylvestris Sw) podem ser associadas ao hidróxido de cálcio. / This study aimed to evaluate the response of rat subcutaneous tissue against calcium hydroxide associated with different substances: chlorhexidine 0.2% in propylene glycol, 0,4% chlorhexidine in propylene glycol, Casearia sylvestris (guaçatonga) in propylene glycol and propylene glycol (control group). The pastes were prepared and placed in polyethylene tubes, which were implanted in the dorsum of each animal (N=18). The experimental periods were 7, 14 and 30 days after surgery, at which time the animals were killed. The samples were histologically processed and stained with hematoxylin and eosin. The evaluation of the responses of connective tissue in contact with the pastes was performed using descriptive and quantitative analyses. The data obtained after the quantitative analysis were subjected to statistical test of Kruskal-Wallis and Dunn with significance level p <0.05. In overall, chlorhexidine 0,4%/calcium hydroxide showed the more intense inflamatory reaction in comparison to the other groups. The extract of Casearia sylvestris showed satisfactory performance in relation to the intensity of the inflammatory reaction, similar to groups 1 and 4. Based on the methodology, it was concluded that at 30 days all the materials do not to interfere with the repair process and that all experimental substances (chlorhexidine 0.4%, 0.2% and the extract of Casearia sylvestris) can be associated with calcium hydroxide.

Submitted by Jean Medeiros (jeanletras@uepb.edu.br) on 2016-04-14T14:53:02Z No. of bitstreams: 1 PDF - Amaro Lafayette Nobre Formiga Filho.pdf: 1284130 bytes, checksum: caeaf28bc01f345af720116bf8d65ead (MD5) / Approved for entry into archive by Secta BC (secta.csu.bc@uepb.edu.br) on 2016-07-22T15:00:45Z (GMT) No. of bitstreams: 1 PDF - Amaro Lafayette Nobre Formiga Filho.pdf: 1284130 bytes, checksum: caeaf28bc01f345af720116bf8d65ead (MD5) / Approved for entry into archive by Secta BC (secta.csu.bc@uepb.edu.br) on 2016-07-22T15:00:53Z (GMT) No. of bitstreams: 1 PDF - Amaro Lafayette Nobre Formiga Filho.pdf: 1284130 bytes, checksum: caeaf28bc01f345af720116bf8d65ead (MD5) / Made available in DSpace on 2016-07-22T15:00:53Z (GMT). No. of bitstreams: 1 PDF - Amaro Lafayette Nobre Formiga Filho.pdf: 1284130 bytes, checksum: caeaf28bc01f345af720116bf8d65ead (MD5) Previous issue date: 2012-07-31 / Coordenação de Aperfeiçoamento de Pessoal de Nível Superior - CAPES / The objective of this study was to evaluate in vitro experimental drugs made from plants of the Brazilian semiarid region, Schinopsis brasiliensis Engl. and Ximenia americana L., comparing its effectiveness with commercial medications. Hydroalcoholic extracts were prepared from six medicinal plants (Schinopsis brasiliensis Engl., Ximenia americana L., Cereus jamacaru DC., Croton campestres ST. Hill, Spondias mombin, Zornia reticulata SM.), which were subjected to an Enterococcus faecalis microbiological screening by agar dilution method, cylinder technique. The two groups of greater efficiency, Schinopsis brasiliensis Engl. and Ximenia americana L., were nebulized and potency was evaluated by the same method. In vitro contamination with E. faecalis was performed in teeth for 60 days with bacterial growth evaluated by scanning electron microscopy, optical microscopy and microbiological tests. After the inoculation period, the teeth were irrigated, dried and filled with different drugs: 1 - experimental S. brasiliensis paste, 2 - experimental X. americana paste, 3 - Calen ® paste and 4 – CTZ paste. The pastes removal and analysis of the results were performed after 8 and 28 days after their application. The results showed that the X. americana paste had greater efficacy and was able to inhibit the growth of E. faecalis after 28 days of treatment, compared with the other studied pastes, with CTZ exception, that presented the best results during the entire treatment period. / O objetivo deste estudo foi avaliar in vitro medicações experimentais formuladas a partir de plantas do semiárido brasileiro, Schinopsis brasiliensis Engl. e Ximenia americana L., comparando sua eficácia com medicações comerciais. Foram preparados inicialmente extratos hidroalcoólicos de seis plantas medicinais (Schinopsis brasiliensis Engl., Ximenia americana L., Cereus jamacaru DC., Croton campestres ST. Hill, Spondias mombin, Zornia reticulata SM.), os quais foram submetidos a um screening microbiológico frente à Enterococcus faecalis pelo método de diluição em ágar, técnica do cilindro. Os dois extratos de maior eficácia, Schinopsis brasiliensis Engl. e Ximenia americana L., foram nebulizados e sua potência foi avaliada através do mesmo método. Em seguida foi realizada a contaminação in vitro dos elementos dentários com E. faecalis por 60 dias, com o crescimento bacteriano comprovado através da Microscopia Eletrônica de Varredura, Microscopia Óptica e de testes microbiológicos. Decorrido o período de inoculação, os elementos dentários foram irrigados, secos e preenchidos com diferentes medicamentos: 1 – pasta experimental de S. brasiliensis, 2 – pasta experimental de X. americana, 3 – pasta comercial Calen ® e 4 – pasta CTZ. A remoção das pastas e a leitura dos resultados foram realizadas após 8 e 28 dias da sua aplicação. Os resultados obtidos mostraram que a pasta formulada a base da X. americana apresentou maior eficácia e foi capaz de inibir o crescimento de E. faecalis após 28 dias de tratamento, quando comparado com as outras pastas testadas, com exceção da CTZ que apresentou os melhores resultados durante todo o período de tratamento.

CoordenaÃÃo de AperfeiÃoamento de Pessoal de NÃvel Superior / A Alpinia zerumbet à uma planta da famÃlia Zingiberaceae, denominada popularmente âcolÃniaâ no Nordeste do Brasil. à utilizada com fins medicinais no tratamento de hipertensÃo e tem sido estudada em relaÃÃo as suas propriedades farmacolÃgicas. Esta pesquisa teve por objetivo analisar o efeito farmacolÃgico das fraÃÃes hexÃnica (FHOEAz), clorofÃrmica (FCOEAz) e metanÃlica (FMOEAz) do Ãleo essencial da Alpinia zerumbet (OEAz) na reatividade vascular in vitro e nos parÃmetros cardiovasculares in vivo. O projeto de pesquisa foi aprovado pela ComissÃo de Ãtica em Pesquisa Animal, da Universidade Federal do CearÃ, sob os protocolos n&#61616; 55/10 e n&#61616; 18/2011, de acordo com as normas de boas prÃticas que envolvem o uso de animais experimentais. Para todos os experimentos foram utilizados ratos Wistar machos. Realizaram-se experimentos de reatividade vascular no banho de ÃrgÃos, com preparaÃÃes de aorta isolada de rato, com endotÃlio Ãntegro e desnudo, mantidos em carbogÃnio e soluÃÃo de Krebs-Henseleit com concentraÃÃo em mmol/L: NaCl: 118,0; KCl: 4,7; KH2PO4: 1,2; MgSO4.7H2O; 1,2; NaHCO3: 15,0; CaCl2: 2,5 e Glicose: 5,5. Foi observada a variaÃÃo da tensÃo isomÃtrica e utilizados diferentes fÃrmacos inibidores especÃficos para anÃlise do mecanismo de aÃÃo do efeito vasodilatador. Analisou-se a pressÃo arterial indireta por pletismografia de cauda em ratos submetidos ao modelo de hipertensÃo por inibiÃÃo crÃnica do Ãxido nÃtrico, atravÃs da administraÃÃo do L-NAME, obtendo-se a pressÃo arterial sistÃlica, diastÃlica e mÃdia, frequÃncia cardÃaca, alÃm da variaÃÃo do peso corporal. Constatou-se que o OEAz, FHOEAz, FCOEAz e FMOEAz induzem relaxamento de anÃis aÃrticos prÃ-contraÃdos com Fenilefrina (1 &#61549;mol/L), de forma dependente da dose, sendo a menor CE50 a da FMOEAz (150,45 &#61549;g/mL). A administraÃÃo por gavagem de 100 mg/kg de OEAz, FHOEAz, FCOEAz e FMOEAz reduziu a pressÃo arterial em ratos hipertensos pelo modelo de inibiÃÃo crÃnica do Ãxido nÃtrico, um efeito que foi superior ao controle negativo com Ãgua destilada e inferior aos controles positivos com Captopril e Nifedipina. A FMOEAz (0,1 - 3000 &#61549;g/mL) induz relaxamento dependente da dose em anÃis aÃrticos prÃ-contraÃdos com Fenilefrina (1 &#61549;mol/L) ou KCl (80 mmol/L), com endotÃlio intacto ou desnudo. Os estudos de mobilizaÃÃo de cÃlcio mostraram que a FMOEAz inibe o influxo de Ca2+ do meio extracelular, bem como interfere na contraÃÃo induzida pela liberaÃÃo de Ca2+ dos estoques intracelulares pela Fenilefrina (1 &#61549;mol/L) ou CafeÃna (30 mmol/L). A 4-aminopiridina (1 mmol/L) e a Iberiotoxina (30 nmol/L) aumentam a CE50 da FMOEAz, mas nÃo interferem no seu efeito vasodilatador final. A prÃ-incubaÃÃo com L-NAME (100 &#61549;mol/L), ODQ (10 &#61549;mol/L); Indometacina (10 &#61549;mol/L), Atropina (1 &#61549;mol/L), Catalase (500 U/ml), SOD (300 U/mL); Wortmannina (0,5 &#61549;mol/L), TetraetilamÃnio (10 mmol/L), Apamina (1 &#61549;mol/L); Caribdotoxina (15 nmol/L) e Glibenclamida (10 &#61549;mol/L) nÃo interferiram no relaxamento induzido pela FMOEAz. Concluiu-se que o OEAz, FHOEAz, FCOEAz e FMOEAz possuem efeito hipotensor in vivo e vasodilatador in vitro, e que o mecanismo de aÃÃo da FMOEAz, provavelmente, envolve o antagonismo aos canais de cÃlcio dependentes de voltagem, aos canais de cÃlcio operados por receptor, interferindo tambÃm na liberaÃÃo de cÃlcio dos estoques intracelulares. / The Alpinia zerumbet is a plant of the family Zingiberaceae, popularly called "colÃnia" in Northeastern Brazil. It is used for medicinal purposes to treat hypertension and has been studied in relation to its pharmacological properties. This study aimed to analyze the pharmacological effect of hexanic (HFEOAz), chloroformic (CFEOAz), and methanolic (MFEOAz) fractions of the essential oil of Alpinia zerumbet (EOAz) in the vascular reactivity in vitro and cardiovascular parameters in vivo. The research project was approved by the Ethics Committee on Animal Research, of the Federal University of CearÃ, under protocol numbers 55/10 and 18/2011, according to the standards of good laboratory practice involving the use of experimental animals. For all experiments male Wistar rats were used. Experiments of vascular reactivity were conducted in organ bath, with preparations of isolated rat aorta with intact endothelium and desnuded, kept in carbogen and Krebs-Henseleit solution with a concentration in mmol/L: NaCl: 118,0; KCl: 4,7; KH2PO4: 1,2; MgSO4.7H2O; 1,2; NaHCO3: 15,0; CaCl2: 2,5 e Glicose: 5,5. Was observed variation in isometric tension and used different specific inhibitors to analyze the mechanism of action of the vasodilator effect. We analyzed blood pressure indirectly by tail plethysmography in rats submitted to chronic hypertension by inhibition of nitric oxide by the administration of L-NAME, obtaining the systolic, diastolic and mean blood pressure, heart rate, beyond variation in body weight. It was found that the EOAz, HFEOAz, CFEOAz MFEOAz induced relaxation of aortic rings pre-contracted with Phenylephrine (1 &#61549;mol/L), of dose-dependent manner, with the smallest of the EC50 MFEOAz (150.45 mg/mL). The administration by gavage of 100 mg/kg EOAz, HFEOAz, CFEOAz MFEOAz reduced blood pressure in hypertensive rats by the model of chronic inhibition of nitric oxide, an effect that was greater than the negative control with distilled water and less than the positive controls with Captopril and Nifedipine. The MFEOAz (0.1 - 3000 Âg/mL) concentration dependently relaxed Phenylephrine (1 &#61549;mol/L) and KCl (80 mmol/L) contracted rings with intact or denuded endothelium. Studies of calcium mobilization showed that FMOEAz inhibits the influx of Ca2+ from the extracellular environment and interferes with the contraction-induced Ca2+ release from intracellular stocks by Phenylephrine (1 &#61549;mol/L) or Caffeine (30 mmol/L). The 4-aminopyridine (1 mmol/L) and Iberiotoxina (30 nmol/L) increased the EC50 of MFEOAz but do not interfere in its final vasodilator effect. The pre-incubation with L-NAME (100 &#61549;mol/L), ODQ (10 &#61549;mol/L), Indomethacin (10 &#61549;mol/L), Atropine (1 &#61549;mol/L), Catalase (500 U/mL), SOD (300 U/mL), Wortmannin (0.5 &#61549;mol/L), Tetraethylammonium (10 mmol/L), Apamin (1 &#61549;mol/L); Caribdotoxin (15 nmol/L) and Glibenclamide (10 &#61549;mol/L) did not interfere with the relaxation induced by MFEOAz. It was concluded that the EOAz, HFEOAz, CFEOAz and MFEOAz have hypotensive effect in vivo and vasodilator effect in vitro, and that the mechanism of action of MFEOAz probably involves the antagonism of calcium channels voltage-dependent, the calcium channel operated by receptor, also interfering in the release of calcium from intracellular stores.