Competition from hardwood species has long been considered a constraint on longleaf pine (Pinus palustris). However, evidence suggests that hardwood midstories may improve longleaf regeneration on xeric sites. Additionally, overstory retention for red-cockaded woodpecker (Leuconotopicus borealis; RCW) habitat may be inadvertently contributing to regeneration failures. Experimental plots in the North Carolina Sandhills were planted with containerized longleaf seedlings across a gradient of overstory density, and hardwoods were chemically controlled by treating at planting, treating after one year, or left untreated. Seedling survival (percent), growth (mass), and brown spot needle blight (percent infected seedlings; BSNB) were measured after two years. Seedling survival, growth, and BSNB infection rates were inversely related to overstory density. Midstory treatments did not significantly affect seedling survival, growth, or BSNB infection rates. Early results did not show facilitative effects from a hardwood midstory, but indicated overstory densities greater than 12 m2/ha negatively impact longleaf regeneration.

No description available.

Understanding forest carbon uptake and associated growth response is important for carbon sequestration and water management practices given the large quantities of carbon stored in forest ecosystems. Climate variability and forest management practices influence the magnitude and rate of soil CO2 efflux; however, their combined effects are complex and not well understood. This study investigated the response of soil CO2 efflux to the combined effects of climate variability, including those caused by climate oscillations, and shelterwood harvesting in a mature temperate white pine (Pinus strobes L.) forest, located near Lake Erie in southern Ontario, Canada. Analyses indicated that local winter temperatures and precipitation were influenced by climate oscillations, which affected forest carbon dynamics. After the shelterwood harvest removed approximately a third of the overstory canopy, no significant differences were found for soil temperature and soil moisture between the pre-harvesting (2008 to 2011) and post-harvesting (2012 to 2014) periods. Despite similar climate conditions pre- and post-harvesting, soil CO2 effluxes post-harvesting were lower. A Gaussian-Gamma specification model determined that heterotrophic (autotrophic) respiration decreased (increased) between pre- and post-harvesting, respectively. Mineral-soil respiration were similar pre- and post-harvesting. Soil CO2 efflux accounted for 78±9% of the annual ecosystem respiration (RE), derived using eddy-covariance fluxes. However, the overall net ecosystem productivity showed no significant difference between pre- and post-harvesting. This was attributed to an increase in the gross ecosystem productivity post-harvesting, compensating for the increased losses (i.e. increased RE). This study highlights the complexities of measuring various components of ecosystem respiration after a disturbance, such as a harvest. The knowledge gained from this study provides a better understanding of climate variability and shelterwood harvesting influences on ecosystem respiration and can be useful for forest managers focused on carbon sequestration and forest conservation. / Dissertation / Doctor of Science (PhD) / Coniferous forest plantations in eastern North America are undergoing silvicultural management to enhance their carbon sequestration capabilities and native-tree species diversity. This study investigated the combined influence of climate oscillations and shelterwood harvesting on soil carbon dynamics of a planted pine forest in southern Ontario, Canada. Between pre- and post-harvesting, soil temperature and soil moisture did not show any significant differences. However, soil CO2 effluxes in post-harvesting years were lower than pre-harvesting years. A Gaussian-Gamma specification model determined that heterotrophic (autotrophic) respiration decreased (increased) post-harvesting and mineral-soil respiration was similar between pre- and post-harvesting. An increase in ecosystem respiration post-harvesting, despite soil CO2 efflux decreasing and being the largest component, was primarily caused by the increase in autotrophic respiration due to enhancement in forest growth. This study improved the understanding of forest carbon dynamics by highlighting the importance of accounting for all components, which may contribute to ecosystem respiration. Results can be useful for forest management practitioners, specifically those focused on carbon sequestration and forest conservation.

Accounting for in-woods carbon storage in carbon accounting systems may be insufficient when substantial amounts of sequestered carbon are harvested and converted to long-lived wood products and landfills. The potential for offsetting greenhouse gas (GHG) emissions by storing carbon in managed loblolly pine forests in the southern United States was projected over the next half-century, both in terms of in-woods aboveground carbon pools and harvested products, including wood used for energy production. A region-wide data set from the Forest Inventory Analysis (FIA) program of USDA Forest Service was used to set initial conditions and estimate model parameters for projecting management activities including plantation area, age distributions of thinning, and clearcut harvest on an annual timestep. The stand-level growth and yield model FASTLOB was linked to the FIA data to project growth rates and annual harvest volumes of sawtimber and pulpwood for the projection period, accounting for annual timber harvests and the life cycles of wood products. In addition to baseline management practices, projections were made for scenarios that assumed increasing management intensities including the use of chemical fertilizers and herbicides and genetically-improved growing stock. Present-day carbon storage in well-managed southern pine plantations averaged 30.54 Mgâ ha⁻¹ (± 2.54%) for aboveground carbon. Over a 50-year projection, annual wood production was 62.1 and 45.9 million green metric tons from pulpwood and sawtimber yield, with roughly one-fourth of the green weight being carbon. Baseline projections showed aboveground carbon pools of up to 341 million metric tons being maintained over the next 50 years, with 93% in aboveground live trees and 7% in coarse woody debris (CWD). The carbon storage in wood products increased steadily over the half-century projection and showed no sign of leveling off, while the storage in plantations was found to remain constant or increase slightly over time. An additional 11 million metric tons of harvested carbon was used for energy per year on average, equivalent to 25% of annual forest-products-industry renewable energy use in U.S.A. Intensified forest management practices showed the potential to increase as much as 30% total carbon stored in in-woods and harvested-wood-products pools, with potential increases up to 40% in energy offsets above the baseline scenario. Reducing management intensity greatly increased in-woods carbon storage potential, but eliminated the wood-products carbon sink. / Ph. D.

Coarse woody debris (CWD) plays an influential role in forested ecosystems by adding organic matter to soils, stabilizing the soil environment, providing wildlife habitat, preventing soil erosion, providing seedling establishment habitat, and involvement in the nutrient cycle. Most CWD research has been conducted in old-growth and unmanaged, second-growth forests. However, less is understood about CWD in intensively managed ecosystems, such as industrialized southern pine plantations. The objectives of this study were to determine the climatic and ecological factors that affect the decomposition rate of CWD, to predict the decomposition rate, specific gravity, and time since death (TSD) using multiple linear regression in industrial loblolly pine (Pinus taeda L.) plantations in the southeastern United States. The study sites for this project were part of a long-term, loblolly pine thinning study maintained by the Loblolly Pine Growth and Yield Research Cooperative at Virginia Tech. Measurements included piece size, position, and decay class. Samples of CWD were collected and analyzed to determine their mass and density. Decomposition rate of CWD was significantly different across position classes and decay classes: disk decomposition rates were significantly negatively correlated with disk diameter, large and small end piece diameter, estimated disk height, and disk dry weight. Average annual precipitation and average annual temperature were not significantly correlated with CWD disk decomposition rate. / Master of Science

Pinus palustris Miller (longleaf pine), in Virginia, is at the northernmost extent of its range. During presettlement times, this species occurred throughout the Piedmont and Coastal Plain of Virginia in pure and mixed stands, covering 607,000 hectares. This forest type has since been reduced to 81 hectares or 0.01% of its former range. Around 5,000 individual Pinus palustris remain on six sites in the coastal plains. Seacock Swamp and Everwoods are both sites known to have naturally regenerated Pinus palustris native to Virginia occurring in mixed-species stands. At both sites, I measured height and diameter of all Pinus palustris and cored individuals greater than 10 cm in diameter at breast height. A total of 71 trees were cored; the cores were crossdated and measured and crossdating was verified with the COFECHA program. A strong competition signal within the tree ring records at both sites signified the importance of stand dynamics on Pinus palustris in second-growth loblolly pine stands. These results are probably due to the mix of species within these stands and competition from loblolly pine as both the dominant understory and overstory species. Using Black and Abrams (2003) boundary line method, we calculated release and suppression events from the tree-ring record over the last century and found a very dynamic system. During the 1950s and 1960s, Seacock Swamp experienced major and moderate releases (23% moderate release and 18% major release in the 1950s and 33% moderate release and 49% major release in the 1960s) in response to a diameter-limit cut in 1953. Other major and moderate releases varied at both sites and may be attributed to different forest management practices that were in place throughout the last century however locating historical land use records to validate this was not possible at the present. / Master of Science

Nine taper equations were tested to predict diameters inside bark along the stem for <i>Pinus oocarpa</i> Schiede trees growing in natural stands of central Honduras. A five parameter submodel predicted as well as an eight parameter model proposed by Kozak, 1988. Taper variation was explored between two geographic regions from which trees with different taper were suspected. Results showed that different site classes, not fully accounted for in the model, might have an effect on the prediction of taper in each region. Also, the effect of crown class and live crown ratio on prediction was evaluated. The model selected exhibited different prediction patterns for dominant and suppressed trees. On the other hand, live crown ratio did not appear to affect prediction. A computer program was written to use the taper equation developed to compute total and merchantable volume to different top diameter limits. / Master of Science

Forest tree breeding methods rank among the most efficient ways to increase productivity and quality of forests. With the advent of high-throughput genotyping technology, genome-enabled breeding has started to gain importance and may overcome some weaknesses of traditional tree breeding. Genomic Selection (GS), which involves using genome-wide markers to predict breeding values of individuals in a population, has been proposed for animal and plant breeding programs. GS enables very accurate selection decisions through estimation of genomic estimated breeding values (GEBVs). While the goal of GS is to predict phenotype from genotype, it does not identify the underlying genes that have important roles in a trait. Genome-Wide Association Studies (GWAS) approaches are therefore complementary to GS, enabling identification of these genes, which may be useful for marker-assisted selection in some traits. In this study, we first estimated heritability for several adaptive traits (cold hardiness, dbh, bud flush, height, and bud set) in a population of Populus trichocarpa and for height, diameter, and stem straightness in Pinus taeda. GEBVs accuracies were estimated using a ridge regression–best linear unbiased prediction (rrBLUP) model, and these accuracies were compared with estimated heritabilities. GWAS was also performed for the both imputed and non–imputed data of P. taeda population using TASSEL (Trait Analysis by aSSociation Evolution and Linkage) software, as well as rrBLUP and FFBSKAT (Fast Family-Based Sequence Kernel Association Test) packages in R. Heritabilities ranged from 0.34 to 0.56 for P. trichocarpa and 0.14 to 0.37 for P.taeda. GWAS identified 3244 associations for dbh, 4077 associations for stem straightness, and 5280 SNPs for height (p≤0.05) in TASSEL using the reduced model (marker data only), whereas 2729, 3272 and 3531 associations were found with the full model where we also included population structure as a covariate. FFBSKAT showed a similar number of SNP associations (2989, 3046 and 3058). There was an inflation of SNP associations (~20k) found in rrBLUP, which suggests population structure was not effectively controlled. The GEBVs accuracies ranged from 0.09 and 0.22 for P.trichocarpa and 0.09 to 0.23 for P.taeda using rrBLUP method. Testing the effect of repetation on the accuracy of GEBV for poplar showed that there was no significant difference between the number of cycles. Also, there was no significant difference the accuracy of GEBVs in pine between two different imputation methods, the marker mean value and Beagle software. / Master of Science

Managed loblolly pine forests comprise an important pool in the global carbon cycle. Understanding the influences upon inputs and outputs of this pool, including the effects of management activities, will allow landowners to understand how carbon can be sequestered in their stands. Specific to this study, we sought to create multivariate models of the output of carbon from the soil in the form of soil CO2 efflux (Rs) and a component of that total efflux, heterotrophic respiration index (Rh), from data collected across the managed range of loblolly pine in the Southeastern U.S. We also performed tests of significance on controlled subsets of these data for the effects of fertilization and of thinning. Finally, we sought a connection between stand leaf area index (LAI) and total soil CO2 efflux or heterotrophic respiration. Our models indicated variability in both Rs and Rh across latitude and physiographic province, respectively, within this range. The Rs (R2 = 0.56) model included temperature, latitude, a soil moisture by temperature effect, soil nitrogen, and bulk density variables. The Rh (R2 = 0.50) model included soil moisture, a temperature by moisture interaction, and physiographic province. Rs was not significantly affected by either fertilization or thinning, yet Rh was influenced by both (negatively and positively, respectively). This indicates a shift in relative contributions of heterotrophic respiration and root respiration components to Rs in response to these treatments. Heterotrophic respiration was shown to have a weak negative response (R2 = 0.04) to increasing stand LAI. / Master of Science

Virginia's only montane pine barren, located in the Warm Springs Mountain Nature Preserve in the Allegheny Highlands of western Virginia is likely threatened by successional changes initiated by a history of fire suppression. Dominated by early successional fire adapted species, such as dwarfed Pinus rigida (Mill.) and Quercus ilicifolia (Wangenh.), this shrubland is home to numerous rare plants and invertebrates. We used vegetation analysis and dendrochronology to document establishment and recruitment patterns and to identify successional trends at this site. Tree establishment of the dominant tree species (P. rigida and Quercus rubra L.) peaked following the last known fire event in the early 1930s. Vegetation analysis revealed an absence of P. rigida seedling recruitment as well as a low density of fire adapted species such as Q. ilicifolia. In contrast, Q. rubra is represented in a variety of age classes and shade tolerant trees such as Acer rubrum (L.) and Pinus strobus (L.) are beginning to establish. A dense understory of ericaceous shrubs and a thick litter layer appear to inhibit recruitment of P. rigida and other early successional species pointing to the need for active management in the form of prescribed burns, which have been effective in other pine barrens. / Master of Science