京都大学 / 新制・課程博士 / 博士(理学) / 甲第23047号 / 理博第4724号 / 新制||理||1677(附属図書館) / 京都大学大学院理学研究科生物科学専攻 / (主査)教授 田村 実, 教授 鹿内 利治, 教授 工藤 洋 / 学位規則第4条第1項該当 / Doctor of Science / Kyoto University / DGAM

The cosmopolitan Pteridium aquilinum (L.) Kuhn is widespread throughout eastern North American, where it is represented primarily by Tryon's (1941) var. latiusculum (Desv.) Underw. and var. pseudocaudatum (Clute) Heller. The taxonomy of Pteridium is controversial. Fourteen isozyme loci and 12 morphological characters were used to assess the taxonomic relationship of these two varieties. Isozyme data indicated a high mean genetic identity (I = 0.976) between eleven bracken populations. Strong patterns of geographic variation for isozyme allele frequencies were also observed. The isozyme results did not separate the two taxa. Numerical analysis of the morphology distinguished the two taxa when the qualitative characters were used alone or in conjunction with some of the quantitative traits. All qualitative characters differed significantly between the two taxa. No perceptible geographic pattern of variation was observed. Morphological distinctiveness was maintained even in those localities where both taxa were present, with few or no intermediates being found. Isozyme evidence suggestive of gene flow between the two varieties was found at Greensboro, NC, where the two morphotypes were easily recognizable. The isozyme evidence strongly indicates conspecificity, while the morphological evidence supports their status at the varietal level. / Master of Science

No description available.

Master of Science / Department of Biology / Carolyn J. Ferguson / The genus Phlox L. presents intriguing opportunities for systematics research, and P. nana is of particular interest. Phlox nana occurs chiefly in mountains of the Chihuahuan desert to northern New Mexico, and it exhibits much morphological variation across its range. Historically, this taxon has been recognized as a single species (sometimes with infraspecific taxa), or as several species. Perhaps most interesting, variation in ploidy level (cytotypic variation) has been evidenced for P. nana. This research employed flow cytometry methods in conjunction with chromosome counts to document patterns of cytotypic variation. Intensive fieldwork in Arizona, New Mexico and Texas enabled excellent sampling, and evaluation of ploidy level for 76 populations was achieved. Diploid and tetraploid chromosome counts were made (four diploid counts; five tetraploid counts), and flow cytometry was conducted on all populations, providing evidence for diploid, tetraploid and hexaploid populations. Polyploids were found to occur in many geographical areas, and in some regions, diploids and polyploids occur in close geographical proximity (e.g., within both the Davis Mountains and the Chisos Mountains of west Texas). Genome size data are presented (with discussion of unusual populations), and geographic patterns of cytotypic variation are presented and discussed. Patterns are also briefly considered with respect to morphology and taxonomy: cytotypic variation does not readily align with historical recognition of taxonomic variation, and this work sets the stage for ongoing, detailed morphometric study. Research on particular species of Phlox benefits from an understanding of a broad phylogenetic context, and low copy nuclear DNA regions are an important resource for phylogeny development. This research further evaluated part of the NADP-dependent isocitrate dehydrogenase gene (idhB) for its usefulness in inferring relationships in Phlox. Samples were PCR amplified for idhB and cloned, and resulting sequences were added to a larger set of idhB sequence data previously developed in the lab. A total of 163 samples were included, and Bayesian Inference and Maximum Parsimony analyses were conducted for complete data sets. Phylogenetic findings are discussed in light of previous work based on chloroplast and high copy nuclear DNA regions, and challenges and utility of using idhB are discussed.

O estudo da família Anacardiaceae tem o objetivo de contribuir com o plano mais amplo do levantamento da \"Flora Fanerogâmica do Estado de São Paulo\", que vem sendo subsidiado pela FAPESP desde 1994 e conta com a colaboração de pesquisadores de muitas universidades e institutos de pesquisa do Estado. As Anacardiaceae compreendem cerca de 81 gêneros e 800 espécies, presentes em ambientes secos a úmidos, principalmente em terras baixas nas regiões tropicais e subtropicais em todo o mundo, estendendo-se até as regiões temperadas. No Brasil a família encontra-se representada por 14 gêneros e 57 espécies. O levantamento das espécies foi realizado baseando-se nas coleções de Anacardiaceae do Estado de São Paulo depositadas nos herbários BHCB, BOTU, ESA, HRCB, MBM, IAC, PMSP, R, RB, SP, SPF, SPFR, SPSF e UEC, expedições de campo e consultas bibliográficas. Os resultados seguem o padrão da série \"Flora Fanerogâmica do Estado de São Paulo\". São apresentadas chaves de identificação para gênero e espécies, descrições morfológicas, ilustrações e dados sobre distribuição geográfica, habitat, variabilidade intraespecífica, período de floração e frutificação, grau de conservação das espécies e riscos de extinção. No Estado de São Paulo, há 12 espécies nativas distribuídas nos gêneros Anacardium, Astronium, Lithrea, Myracrodruon, Schinus, Spondias e Tapirira. Lithrea molleoides, Schinus terebinthifolius e Tapirira guianensis são as espécies mais amplamente distribuídas da família no Estado, sendo encontradas em quase todas as formações vegetacionais, inclusive em áreas antropizadas. Spondias mombin é encontrada na floresta Estacional Semidecidual e matas ciliares próximas ao rio Paraná, nas regiões noroeste e oeste do Estado. Schinus engleri é encontrada, no Estado de São Paulo, nas florestas Ombrófila Mista Alto-Montana de Campos do Jordão e da Serra da Bocaina e Schinus weinmannifolius ocorre nos cerrados e campos de Itararé e Itapeva, entre outros municípios localizados próximos à região sul-sudoeste do Estado de São Paulo. O gênero Anacardium é representado no Estado pelas espécies A. humile, planta com hábito geoxílico comum nos cerrados e A. occidentale, uma árvore pequena das restingas. Astronium graveolens e Myracrodruon urundeuva, assim como a maioria das espécies de Anacardiaceae, ocorrem nas Florestas Estacionais Semideciduais e nos cerrados do Estado de São Paulo. Um padrão de distribuição incomum é observado em Lithrea brasiliensis que, com exceção do material-tipo, não possui exemplares ulteriores do Estado de São Paulo depositados nos herbários consultados. Em relação ao grau de conservação das espécies, Lithrea brasiliensis deve ser categorizada como presumivelmente extinta (EX) e Myracrodruon urundeuva como quase ameaçada (NT), as demais espécies enquadram-se como espécies não ameaçadas, na categoria de preocupação menor (LC) / The present study on Anacardiaceae aims to contribute with the broader survey project of the Phanerogamic flora of the São Paulo state which. This great efford has been supported by FAPESP since 1993 and counts on the collaboration of researchers from many universities and institutes of the state. The family comprises ca. 81 genera and 800 species distributed mostly in lowlands, from dry to moist habitats, throughout the tropics and subtropics worldwide and also extending into the temperate zone. Iin Brazil Anacardiaceae is represented by 14 genera and 57 species. The present survey was carried out based on field work, bibliographical references on Anacardiaceae and examination of the collections from the following herbaria: BHCB, BOTU, ESA, HRCB, MBM, IAC, PMSP, R, RB, SP, SPF, SPFR, SPSF, UEC. The results are presented following the Phanerogamic flora of Sao Paulo State. We provide identification keys to genera and species, morphological descriptions, illustrations and additional data on distribution, habitat, intraespecific variability, periods of flowering and fruiting, species conservation status. In the area this family is represented by 12 native species belonging Anacardium, Astronium, Lithrea, Myracrodruon, Schinus, Spondias e Tapirira. Lithrea molleoides, Schinus terebinthifolius and Tapirira guianensis are the most common and widespread Anacardiaceae species of São Paulo state and they are collected in almost all types of vegetation, including disturbed areas. Spondias mombin occurs in Sazonal Semidecidous and Riparian Forests next to the Paraná River in the northwest and the west regions of the state. S. engleri is reported for São Paulo state in the High-Montane Mixed Ombrophylous Forest of Campos do Jordão and Serra da Bocaina and S. weinmannifolius is found in the \"cerrado\" and \"campos\" of Itararé, Itapeva and other cities located next to the south and the south-west regions of the state. Anacardium is represented in the state by two species, A. humile, a \"cerrado\" common plant with geoxylic habit, and A. occidentale, a \"restinga\" small tree. Astronium graveolens and Myracrodruon urundeuva, as well as the majority of Anacardiaceae species are collected in Sazonal Semidecidous Forests and \"cerrados\" of São Paulo state. An uncommon distribution pattern is found in Lithrea brasiliensis, which is reported for São Paulo state only from type-material. In relation to the species conservation status, Lithrea brasiliensis should be categorized as Presumably Extinct (EX) and Myracrodruon urundeuva as Near Threatened (NT). The other species are classified as Least Concern (LC) category

A revision of the genus Chamaecrista (Leguminosae) in Ecuador is presented. The work is based on morphometric studies of herbarium material and information from the literature and the Internet. The purpose of the study was to get a better knowledge of the distribution, taxonomic status and conservation of Chamaecrista in Ecuador. The study recognizes in all six species and several varieties, viz. Ch. nictitans with var. jaliscensis, var. disandea, var. pilosa, var. paraguariensis and var. glabrata, Ch. glandulosa with var. flavicoma and var. andicola, Ch. absus and Ch. rotundifolia. Keys, descriptions and illustrations are provided for all taxa.

Whole document restricted, see Access Instructions file below for details of how to access the print copy. / This work constitutes a detailed study of the spores, of the ferns and fern allies, both native and adventive, which grow in New Zealand, from the Kermadec Islands in the North, to the Chatham Islands in the East and the Subantarctic Islands in the South. Twenty live families with sixty five genera, two hundred and eleven species (of which c.20 are introduced) and three subspecies are included. Seven species are heterosporous the remainder are homosporous. Trilete spores are found in c.104 species representing c.30 genera. Perine is present in most taxa (with the exception of Gleichenia) and ranges from the large and sac-like form as seen in Cyathea smithii, to the thin and reduced form seen in Adiantum. Monolete spores are found in c.109 species representing c.37 genera (two genera are included twice, Isoetes which has trilete megaspores and monolete microspores and Lindsaea which has both monolete and trilete species). Perine is present in most taxa (except Sticherus spp.) and ranges from a fine deposit as seen in the Psilotaceae, to an enlarged form, heavily winged, as seen in the Aspleniaceae. Sculpture in all taxa (with the exception of the Lycopodiaceae and some members of the Ophioglossaceae, which may have ornamentation distributed distally), is present on both distal and proximal faces. One adventive taxon Equisetum arvense L. has an unusual circular laesura and elaborate elaters. Light micrographs of acetolysed and fresh spores along with scanning electron micrographs, are included for each taxon. Keys presented, are based on gross spore morphology and are applicable to fresh and acetolysed material. Descriptions include a list of synonymous species, details of spore shape, laesura/ae details, perine/exine sculpture and thickness (where sections allow), size (measured from n=50 spores per population), for samples treated with cotton blue (lactophenol aniline blue formula), mounted in glycerine jelly and acetolysed samples, mounted in silicone oil. Percentage size differences for acetolysed material mounted in glycerine jelly are also noted, along with previous spore dimensions recorded in the literature. Descriptions also contain chromosome number, where known, a list of previously recorded descriptions, a brief indication of geographic location and a list of vouchers for each sample. Experiments on the size and morphology of fern spores in reaction to different preparation techniques are discussed. Fresh spores of seven species, four trilete (Adiantum fulvum, Cyathea smithii, Hymenophyllum flabellatum and Lycopodium volubile) and three monolete (Blechnum chambersii, Paesia scaberula and Tmesipteris elongata), were assessed wth regard to the effect of three standard pretreatments (lactophenol aniline blue, 10% KOH, acetolysis) and two mounting media (glycerine jelly and silicone oil). Changes in morphology and size of the various wall layers were noted in comparison to spores observed fresh in water. Results indicate that variation includes shrinkage e.g. silicone oil and expansion of both exine and perine in glycerine jelly. Both effects are modified by previous treatments.

The role of, and response to, disturbance, in the dynamics of the mountain beech forest at western Mt Ruapehu, was examined, using palynology, dendrochronology, and vegetation survey. The pollen record indicates that, two thousand years ago, the west Ruapehu forest was dominated by Nothofagus solandri. About 1800 years B.P., the eruption of Lake Taupo devastated the forest. Though Halocarpus spp. and Phyllocladus asplenifolius were important early colonizers, Nothofagus solandri re-established itself close to its present limits after the eruption. Fire, from about 650 years ago onwards, did not affect the beech forest, but did affect the coniferous vegetation on the ring plain near the mountain. The age structure of the forest shows that there was a large disturbance event shortly before 1740. A dieback occurring about 1969 appears to have been the largest episode of mortality since 1740, larger than the dieback episode described by Cockayne at the beginning of this century. Dieback occurred as a short peak-period of Nothofagus solandri death about 1969. It was the large trees of the mature cohort which tended to be killed by dieback rather than the smaller individuals. Tree-ring analysis, showed that narrow tree-rings occurred in beech in the 1960's. Extreme rainfall years in the early 1960's may have put the beech trees under stress, making them susceptible to dieback. The drought in 1969 may have precipitated extensive mortality. The characteristics of N. solandri rings from Ruapehu are similar to ring characteristics of that species from South Island studies. The 1982 cyclone had a noticeable impact on the forest, affecting some areas severely. However, the impact was relatively minor compared to the influence of dieback. The mean density of trees >= 10cm dbh was 520 stems/ha., of which 220 stems/ha. were Nothofagus solandri. The mean basal area was 22m2 /ha., of which 11m2 /ha. was N. solandri. The basal area is very low compared to that in other N.Z. forests. N. solandri, Griselinia littoralis, Phyllocladus asplenifolius, Podocarpus hallii, and Libocedrus bidwillii, made up 35, 22, 16, 9, and 8 percent, respectively, of the total density. Basal area, density, and species composition varied as much within sites, as between them. The shrub layer formed a large part of the vegetation at west Ruapehu. Coprosma species were particularly abundant. There is sufficient regeneration to indicate that a new cohort of N. solandri is becoming established in most areas. Some areas will probably remain in shrubland for many decades. The result of dieback and windthrow has been to virtually eliminate the canopy of large old beech trees, and to increase the heterogeneity of stand structure. N. solandri seedling densities are not as high as those found in many South Island beech forests. Nothofagus solandri is growing faster than are most of the co-existing tree species, and it appears likely that it will continue as the dominant tree species. Implications for forest management are discussed.

Whole document restricted, see Access Instructions file below for details of how to access the print copy. / This work constitutes a detailed study of the spores, of the ferns and fern allies, both native and adventive, which grow in New Zealand, from the Kermadec Islands in the North, to the Chatham Islands in the East and the Subantarctic Islands in the South. Twenty live families with sixty five genera, two hundred and eleven species (of which c.20 are introduced) and three subspecies are included. Seven species are heterosporous the remainder are homosporous. Trilete spores are found in c.104 species representing c.30 genera. Perine is present in most taxa (with the exception of Gleichenia) and ranges from the large and sac-like form as seen in Cyathea smithii, to the thin and reduced form seen in Adiantum. Monolete spores are found in c.109 species representing c.37 genera (two genera are included twice, Isoetes which has trilete megaspores and monolete microspores and Lindsaea which has both monolete and trilete species). Perine is present in most taxa (except Sticherus spp.) and ranges from a fine deposit as seen in the Psilotaceae, to an enlarged form, heavily winged, as seen in the Aspleniaceae. Sculpture in all taxa (with the exception of the Lycopodiaceae and some members of the Ophioglossaceae, which may have ornamentation distributed distally), is present on both distal and proximal faces. One adventive taxon Equisetum arvense L. has an unusual circular laesura and elaborate elaters. Light micrographs of acetolysed and fresh spores along with scanning electron micrographs, are included for each taxon. Keys presented, are based on gross spore morphology and are applicable to fresh and acetolysed material. Descriptions include a list of synonymous species, details of spore shape, laesura/ae details, perine/exine sculpture and thickness (where sections allow), size (measured from n=50 spores per population), for samples treated with cotton blue (lactophenol aniline blue formula), mounted in glycerine jelly and acetolysed samples, mounted in silicone oil. Percentage size differences for acetolysed material mounted in glycerine jelly are also noted, along with previous spore dimensions recorded in the literature. Descriptions also contain chromosome number, where known, a list of previously recorded descriptions, a brief indication of geographic location and a list of vouchers for each sample. Experiments on the size and morphology of fern spores in reaction to different preparation techniques are discussed. Fresh spores of seven species, four trilete (Adiantum fulvum, Cyathea smithii, Hymenophyllum flabellatum and Lycopodium volubile) and three monolete (Blechnum chambersii, Paesia scaberula and Tmesipteris elongata), were assessed wth regard to the effect of three standard pretreatments (lactophenol aniline blue, 10% KOH, acetolysis) and two mounting media (glycerine jelly and silicone oil). Changes in morphology and size of the various wall layers were noted in comparison to spores observed fresh in water. Results indicate that variation includes shrinkage e.g. silicone oil and expansion of both exine and perine in glycerine jelly. Both effects are modified by previous treatments.

The role of, and response to, disturbance, in the dynamics of the mountain beech forest at western Mt Ruapehu, was examined, using palynology, dendrochronology, and vegetation survey. The pollen record indicates that, two thousand years ago, the west Ruapehu forest was dominated by Nothofagus solandri. About 1800 years B.P., the eruption of Lake Taupo devastated the forest. Though Halocarpus spp. and Phyllocladus asplenifolius were important early colonizers, Nothofagus solandri re-established itself close to its present limits after the eruption. Fire, from about 650 years ago onwards, did not affect the beech forest, but did affect the coniferous vegetation on the ring plain near the mountain. The age structure of the forest shows that there was a large disturbance event shortly before 1740. A dieback occurring about 1969 appears to have been the largest episode of mortality since 1740, larger than the dieback episode described by Cockayne at the beginning of this century. Dieback occurred as a short peak-period of Nothofagus solandri death about 1969. It was the large trees of the mature cohort which tended to be killed by dieback rather than the smaller individuals. Tree-ring analysis, showed that narrow tree-rings occurred in beech in the 1960's. Extreme rainfall years in the early 1960's may have put the beech trees under stress, making them susceptible to dieback. The drought in 1969 may have precipitated extensive mortality. The characteristics of N. solandri rings from Ruapehu are similar to ring characteristics of that species from South Island studies. The 1982 cyclone had a noticeable impact on the forest, affecting some areas severely. However, the impact was relatively minor compared to the influence of dieback. The mean density of trees >= 10cm dbh was 520 stems/ha., of which 220 stems/ha. were Nothofagus solandri. The mean basal area was 22m2 /ha., of which 11m2 /ha. was N. solandri. The basal area is very low compared to that in other N.Z. forests. N. solandri, Griselinia littoralis, Phyllocladus asplenifolius, Podocarpus hallii, and Libocedrus bidwillii, made up 35, 22, 16, 9, and 8 percent, respectively, of the total density. Basal area, density, and species composition varied as much within sites, as between them. The shrub layer formed a large part of the vegetation at west Ruapehu. Coprosma species were particularly abundant. There is sufficient regeneration to indicate that a new cohort of N. solandri is becoming established in most areas. Some areas will probably remain in shrubland for many decades. The result of dieback and windthrow has been to virtually eliminate the canopy of large old beech trees, and to increase the heterogeneity of stand structure. N. solandri seedling densities are not as high as those found in many South Island beech forests. Nothofagus solandri is growing faster than are most of the co-existing tree species, and it appears likely that it will continue as the dominant tree species. Implications for forest management are discussed.