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1	BIOINFORMATIC ANALYSIS OF A MAMMALIAN BIP GENE FOR INSERTION INTO GREEN ALGAE AND COMPARISON OF ITS POSSIBLE EFFECTS ON THE SYNTHESIS OF A MAMMALIAN ANTIBODY Ghazanfar, Katrina 23 September 2004 (has links) This dissertation describes a study utilizing bioinformatics to analyze homologues of a molecular chaperone, glucose-regulated protein 78 (grp 78), also known as BiP. The selected homologous proteins originate from organisms of infinitely diverse genera. Comparisons of protein sequence yielded the first clues of a common ancestry among these proteins. Furthermore, protein molecular weights, isoelectric points, N-terminal amino acids and half-lives of a known homolog and a non-homologous protein were examined. Additionally, electroporation, a state-of-the-art plasmid insertion technique, was explored using Chlamydomonas reinhardtii, a green alga, as the recipient of a parent plasmid, pSP124S. Distinctive hypertonic solutions and three separate field strengths were used in the plasmolysis of the cell wall of C. reinhardtii and subsequent electroporation, respectively. The number of transformants was tallied to evaluate which electroporation condition would yield the most transformed colonies. We had two discrete hypotheses: 1) that a structurally and functionally similar protein to glucose-regulated protein 78 exists across a wide spectrum of organisms and 2) that Chlamydomonas reinhardtii could be successfully transformed with pSP124S under certain electroporation conditions. The bioinformatics investigation revealed that analogous proteins to Human GRP 78 existed in Mus musculus (mouse), Rattus norvegicus (rat), Gallus domesticus (chicken), Gallus domesticus (chicken), Mesocricetus auratus (golden hamster), Bos taurus (cow), Xenopus laevis (frog), and Spinacia oleracea (spinach). Moreover, these homologous proteins more likely have a common evolutionary origin. BIP Bioinformatics HuCC49 Chlamydomonas reinhardtii GRP 78 pSP124S electroporation plasmolysis Medicine and Health Sciences
2	Determination of Membrane Fluidity And Correlate Its Effect in Bulk Bacterial Cell Respiration Ojha, Krishna Raj 15 July 2020 (has links) No description available. Chemistry E coli fluidity of a membrane plasmolysis confocal microscope fluorophores cell respiration plasmid construction gene deletion
3	Přízemní ozon jako jeden z faktorů oxidativního stresu v podmínkách horských lesů. / Surface ozone as a factor of oxidative stress in mountain forests. Bendáková, Hana January 2011 (has links) This study presents mountain forest Norway spruce (Picea abies) injured by surface ozone and oxidative stress. Norway spruce is not a sensitive species but it is the most representative tree in our country and this is the reason to pay attention on its injury. Study was connected to the ozone measurement by CHMI in Jizerske mountains. Aim of our study was to find influence of surface ozone on the forest and show correlation between ozone concentrations, altitude and Norway spruce injury. Ozone concentrations are growing with the altitude. Increasing percentage of injury with growing concentrations and altitude was expected. Needles were collected at the altitude 750-1100 meters. Collected needle years were 2006, 2007 and 2009. The 2009 year was not evaluated because no symptoms were found. Injury was observed on the maroscopical, microscopical and biochemical level. Visual injury was assessed by percentage of injured needle surface by chlorosis and necrosis. Microscopical analysis studied injury on cross and longitudinal sections of needles. On the longitudinal sections were visible typically bone-cells. These cells have lost its shape by ozone stress and were narrowed in its width. Bone-cells were best visible near the stomata. Four symptoms were observed on the cross sections: tannin...
4	Ausgewählte Eigenschaften des Sporopollenins der Kiefer Bohne, Guido 27 February 2007 (has links) Gegenstand der Arbeit sind Zusammenhänge zwischen physikochemischen Eigenschaften und Funktionen der Exine bei Ausbreitung, Bestäubung und Befruchtung. Dabei bewährte sich der Einsatz der 3-kammrigen Sporopolleninkapseln (Zentralkapsel und Sacci) in der Permeationschromatographie. Sowohl kinetisch bedingte chromatographische Dispersion kleiner Moleküle als auch Konzentrationsänderungen von Zuckern und Dextranmolekülen im Medium wurden zur Bestimmung von Permeabilitätskoeffizienten der Nexine genutzt. Die Wasserabsorptionskapazität von Exinefragmenten und die hydraulische Leitfähigkeit der Nexine wurden anhand von Konzentrationsänderungen ausgeschlossener Dextranmoleküle ermittelt. Das Tectum der saccalen Sexine ist eine Mikrofiltermembran mit scharfer Trenngrenze im Submikrometerbereich; daher werden an den Sacci nur Hydrokolloide mit Stokes''schen Radius über 100 nm (z.B. aus nativem Dextran) ausgeschlossen. Die Nexine ist eine nicht-ideale Umkehrosmose-Membran, die in Zucker- und Salzlösungen hohe Reflexionskoeffizienten zeigt; zusätzlich besitzt sie wenige große Poren, die den Austausch von Zuckern und selbst kleinen Polymermolekülen ermöglichen. Die hydraulische Leitfähigkeit der Nexine liegt im Größenbereich derjenigen von Plasmamembranen (0,39-0,48 µm s-1 MPa-1); die Ergebnisse zeigen, dass die Exine weder die Nährstoffaufnahme des Sporoplasten aus der lokulären Flüssigkeit noch dessen rasche Rehydratation in der Mikropyle behindert. Die Einfaltungen der distalen Nexine (oberhalb der Sacci) und die Omega-Faltung der Exine zwischen den Sacci (Leptom) bieten beim Quellvorgang Schutz vor zu schneller Flächenausdehnung der Plasmamembran. Der Corpus kann mit konzentrierten Elektrolytlösungen beladen werden. Beim anschließenden osmotischen Schwellen in Wasser reißt die Exine, und der Sporoplast wird mit anhaftender Intine ausgeschleudert. Wasser und andere polare Flüssigkeiten adhärieren stärker als hydrophobe Flüssigkeiten an Sporopollenin. Die Sporopolleninmatrix weist eine hohe Feststoffdichte auf, ist wenig quellfähig (0,18 mL g-1 TM) und deformationsstabil. Dies ermöglicht die Pulverbildung beim Trocknen. / Subject of this thesis are relationships between physicochemical properties and functions of the exine concerning propagation, pollination and fecundation. Here the application of the 3-chambered sporopollenin-microcapsules (central capsule and sacci) in permeation chromatography proved of value. Both the kinetically dependent dispersion of small molecules and changes in concentration of sugars and dextran molecules in the medium were analysed to determine permeability coefficients of the nexine. The water absorption capacity of exine fragments and the hydraulic conductance of the nexine were calculated by means of changes in concentrations of excluded dextran molecules. The tectum of the saccal sexine is a microfiltration membrane with a sharp cut off in the submicrometer range; thus hydrocolloids with Stokes´radii over 100 nm (e.g. from native dextran) are excluded from the sacci. The nexine is a non-ideal reverse osmosis membrane having high reflexion coefficients in sugar and salt solutions; in addition few large pores allow the exchange of sugars and even of small polymers. The hydraulic conductance of the nexine is in the range typically for plasmamembranes (0.39-0.48 µm s-1 MPa-1); the results indicate that the exine does neither obstruct the uptake of nutrients by the sporoplast from the locular fluid nor hinder the rapid rehydration in the micropyle. When rehydrating, the distal foldings of the nexine (above the sacci) and the omega-like folding of the exine between the sacci (leptom), provide protection for the plasmamembrane when its surface area has to increase too rapidly. The corpus can be loaded with a concentrated electrolyte solution. When subsequently transferred into water the exine rupture and the sporoplast along with the intact intine is ejected. Water and other polar liquids adhere stronger to sporopollenin than hydrophobic ones. The matrix of sporopollenin show a high density in its solid content, water absorption capacity is low (0.18 mL g-1 DM) and it is resistant to deformation. This enable the formation of powder while dehydrating. Diffusion Plasmamembran Benetzbarkeit Calcium Chromatographie Deplasmolyse Dextran Evans Blau Exine Exocytose Größenausschluss HETP hydraulische Leitfähigkeit hypo-osmotischer Schock Intine Kiefer Leptom Nexine osmotischer Schock Parenchymzellen Permeabilität Pinus sylvestris L. Pinus nigra Arnold Plasmoptyse Pollen Poren Reflexionskoeffizient Sexine Sporopollenin Ultrafilterkoeffizient Wasserabsorption Wasseraufnahme Zucker diffusion plasma membrane exocytosis Calcium chromatography de-plasmolysis dextran Evans Blue exine HETP hydraulic conductivity hypo-osmotic shock intine leptom nexine osmotic shock parenchyma cells permeability pine Pinus nigra Arnold Pinus sylvestris L. plasmoptysis pollen pores reflection coefficient sexine size exclusion sporopollenin sugars ultrafilter coefficient water absorption water uptake wettability 570 Biowissenschaften, Biologie 32 Biologie WL 7950 WN 7400 ddc:570

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