Seminario para optar al grado de Ingeniero Comercial, Mención Administración / El año 2011 el Chile se vio fuertemente remecido por el denominado Caso La Polar. El caso adquirió notoriedad pública por las prácticas de dudosa reputación que se habían desarrollado en la compañía, las que consistían en repactaciones unilaterales de clientes altamente riesgosos, falseamiento de estados de resultados y otras irregulares prácticas administrativas. Creemos que este caso es importante para investigar y analizar por el hecho de que miles de personas, tanto clientes como trabajadores, se vieron involucrados, así como también por lo que representa el caso para la credibilidad y legitimidad de las actuales estructuras de la sociedad. La propuesta de nuestro trabajo está centrada en entender el fenómeno de la corrupción organizacional desde una perspectiva psico-social, buscando integrar de la mejor forma el caudal de información disponible con el objetivo de desarrollar conclusiones transversales que nos ayuden a entender el fenómeno acontecido con una visión amplia, sustentada por lo acontecido dentro de La Polar

The spins of planetary bodies are not stagnant; they evolve in response to both external and internal forces. One way a planet's spin can change is through true polar wander. True polar wander is the reorientation of a planetary body with respect to its angular momentum vector, and occurs when mass is redistributed within the body, changing its principal axes of inertia. True polar wander can literally reshape a world, and has important implications for a variety of processes—from the long-term stability of polar volatiles in the permanently shadowed regions of airless worlds like the Moon and Mercury, to the global tectonic patterns of icy worlds like Pluto. In this dissertation, we investigate three specific instances of planetary true polar wander, and their associated consequences. In Chapter 2 we investigate the classic problem of the Moon's dynamical figure. By considering the effects of a fossil figure supported by an elastic lithosphere, and the contribution of impact basins to the figure, we find that the lunar figure is consistent with the Moon's lithosphere freezing in when the Moon was much closer to the Earth, on a low eccentricity synchronous orbit. The South Pole-Aitken impact basin is the single largest perturbation to the Moon’s figure and resulted in tens of degrees of true polar wander after its formation. In Chapter 3 we continue our analyses of the lunar figure in light of the discovery of a lunar ”volatile" paleopole, preserved in the distribution of hydrogen near the Moon's poles. We find that the formation and evolution of the Procellarum KREEP Terrain significantly altered the Moon’s orientation, implying that some fraction of the Moon’s polar volatiles are ancient—predating the geologic activity within the Procellarum region. In Chapter 4 we investigate how the formation of the giant, basin-filling glacier, Sputnik Planitia reoriented Pluto. This reorientation is recorded in both the present- day location of Sputnik Planitia (near the Pluto-Charon tidal axis), and the tectonic record of Pluto. This reorientation likely reflects a coupling between Pluto’s volatile cycles and rotational dynamics, and may be active on other worlds with comparably large, mobile volatile reservoirs. Finally, in Chapter 5 we consider the broader context of these studies, and touch on future investigations of true polar wander on Mercury, Venus, Mars, Vesta, Ceres, and other worlds in our solar system.

The Fourier transform is one of the most useful tools in science and engineering and can be expanded to multi-dimensions and curvilinear coordinates. Multidimensional Fourier transforms are widely used in image processing, tomographic reconstructions and in fact any application that requires a multidimensional convolution. By examining a function in the frequency domain, additional information and insights may be obtained. In this thesis, the development of a symbolic computer algebra toolbox to compute two dimensional Fourier transforms in polar coordinates is discussed. Among the many operations implemented in this toolbox are different types of convolutions and procedures that allow for managing the toolbox effectively. The implementation of the two dimensional Fourier transform in polar coordinates within the toolbox is shown to be a combination of two significantly simpler transforms. The toolbox is also tested throughout the thesis to verify its capabilities.

Polar body emission (PBE), a form of asymmetric division, occurs twice during vertebrate oocyte maturation and is required to produce a haploid egg for sexual reproduction. Our lab elucidated parts of the mechanism that regulates PBE in Xenopus laevis oocytes. Cdc42 and RhoA, two GTPases, were shown to mediate membrane protrusion and the contractile ring, respectively. It is believed that cdc42 is mediating the protrusion by regulating actin polymerization. However, it is not clear what upstream signaling pathway regulates cdc42 activation during PBE. One possibility is calcium signaling, which occurs at fertilization, and is required for second PBE. Interestingly, the fertilization calcium transient also regulates cortical granule exocytosis/membrane retrieval, a process that also involves cdc42-mediated actin assembly. Furthermore, active cdc42 and RhoA are found in non-overlapping concentric zones in single-cell wound healing; their activation requires calcium signaling. To determine possible calcium transients during polar body emission, we employed the calcium-binding C2 domain of PKCβ in live cell imaging. Surprisingly, the most prominent C2 signal was seen after cdc42 activation and membrane protrusion. Co-localization experiments indicated that the C2 signal appeared at the cortical area marked by the contractile ring component anillin, and after partial constriction of the ring. Injection of the calcium chelator, dibromo-BAPTA, abolished the C2 signal, suggesting that it is indeed depicting a calcium transient. Dibromo-BAPTA injection also inhibited polar body abscission, as assessed by a novel abscission assay developed in our lab. We have for the first time detected a calcium signal during PBE that is essential to the last step of cytokinesis—abscission.

Over the past 20 years numerous major arbovirus outbreaks including Yellow Fever virus, West Nile virus, dengue virus, Chikungunya virus, and most recently Zika virus, have highlighted the need for novel methods of control for these diseases. Wolbachia are maternally transmitted endosymbionts that inhabit a large portion of arthropods, including Aedes aegypti, Aedes albopictus, and Culex pipiens, which are three mosquito vectors that transmit these viruses. Wolbachia have been shown to reduce vector competency in these mosquitos. However, the molecular pathways at the Wolbachia-host cell interface are mostly unknown. For fundamental biological questions and for vector control approaches, there is a need to further our understanding of host-symbiont interactions at the molecular level. From this, autophagy has been suggested as one possible mechanism at this interface. Autophagy is a conserved, cellular, homeostasis process that involves degrading cytoplasmic contents, including organelles and protein aggregates. Canonical autophagy can be a large, bulky process, or a more targeted, selective one. There is the potential for Wolbachia to both benefit from the nutrients generated from bulk autophagy, but also be targeted in the host’s immune response, as selective autophagy has been shown to aid in removal of intracellular pathogens. Here we describe how autophagy plays a role in regulating Wolbachia in Drosophila melanogaster as a model for mosquitos. Using antibody and FISH staining we visualized differences in Wolbachia density after knocking down autophagy genes. Knocking down Atg1, an important factor in initiating autophagy, was sufficient for increasing Wolbachia density in polar cells, but other components like Atg7 and Ref(2)p were not. Results varied based on the Wolbachia strain infection, showing that autophagy differentially affects Wolbachia according to the strain. These findings further our understanding of molecular host-symbiont interactions and provide additional tools to Wolbachia based vector control strategies. / 2021-10-09T00:00:00Z

Thesis (M. Sc. (Animal Production)) -- University of Limpopo, 2015 / The objectives of this study were: 1) To determine the effect of oocyte retrieval techniques (slicing and aspiration) on the quality and quantity of cattle oocytes, 2) To evaluate the effect of different concentrations of hormones on the maturational rate of cattle oocytes selected by brilliant cresyl blue staining, 3) To evaluate fertilization rate and cleavage/embryonic development of oocytes with or without cumulus cells, and 4) To compare the effect of fresh and frozen thawed semen on the fertilization rate of cattle oocytes. In Experiment 1: oocytes were recovered from abattoir derived ovaries using slicing and aspiration. The recovered oocytes were exposed for 90 minutes to 26μM of brilliant cresyl blue (BCB) stain and classified according to the colour of their cytoplasm: BCB+ (oocytes with blue cytoplasm) and BCB- (unstained oocytes). There was no difference (P>0.05) in the quality of oocytes recovered using slicing (60.7 %) or aspiration (53.7 %) techniques. In experiment 2: The BCB selected and the non-selected immature oocytes were randomly allocated into medium 199 + 10 % fetal bovine serum (FBS) maturation media. The media was supplemented with three different concentrations of hormones as treatments (T). The T1 (0.5 μg/ml of follicle stimulating hormone (FSH), 5mg/ml of luteinizing hormone (LH) and 2 μg/ml of estradiol (E2) as the control group. Then, T2 (1 μg/ml of FSH, 6 mg/ml of LH and 2.5 μg/ml of E2) and T3 (1.5 μg/ml of FSH, 7 mg/ml of LH and 4.5 μg/ml of E2). Maturation rate of oocytes was determined by the protrusion of the first polar bodies 24 hours following in vitro maturation. Treatment 2 yielded higher (P<0.05) maturation rate for both BCB+ (65.6 %) and without BCB (60.3 %) oocytes with T1 giving lower (P<0.05) maturation rate for BCB+ (22 %) and without BCB (16 %) oocytes. However, BCB- oocytes had lower (P<0.05) polar body extrusion (3.03 %, 8.1 % and 2.2 %) for T1, T2 and T3, respectively. In Experiment 3: one group of the presumptive zygotes was denuded of cumulus cells and the other group was cultured with cumulus cells. The presumptive zygotes were in vitro cultured in SOF-BSA and changed to SOF-FBS after 48 hours. High fertilization/cleavage rate was observed in oocytes cultured with cumulus cells (29.0 %) compared to the denuded oocytes (20.0 %) for 2-4 cells stage. Day 7 blastocysts were more (P<0.05) on oocytes cultured with cumulus cells (32 %) compared to denuded oocytes (13 %). In experiment 4: The matured oocytes were fertilized using fresh and frozen thawed semen. The oocytes fertilized with frozen thawed semen obtained a better number of 2-4 cell cleavage (23 %) when compared to fresh semen (19 %). Oocytes that were fertilized with frozen thawed semen also obtained higher morula (13 %) and blastocyst (8 %) compared to fresh semen with morula (3.4 %) and blastocyst (2 %). In conclusion, immature oocytes that were exposed to BCB+ and cultured in M199 supplemented with 10 % FBS, 0.5 μg/ml of FSH, 5 mg/ml of LH and 2 μg/ml of E2 had a higher (P<0.05) number of matured oocytes (extrusion of first polar body) compared to those that were not exposed to BCB (no BCB). Oocytes that were cultured with cumulus cells yielded a higher (P<0.05) number of cleaved embryos compared to the denuded oocytes. Slicing yielded a higher (P<0.05) number of oocytes, however the quality of oocytes recovered was similar compared to those recovered by the aspiration technique (P>0.05). Oocytes fertilized with frozen thawed semen yielded higher (P<0.05) number of 2-4 cell, morula and blastocyst when compared with oocytes that were fertilized using fresh semen. Keywords: ovaries, oocytes, slicing, aspiration, COCs, BCB, polar body and cattle

A flight data acquisition system was developed to aid unmanned vehicle designers in verifying the vehicle's design performance. The system is reconfigurable and allows the designer to choose the correct combination of complexity, risk, and cost for a given flight test. The designer can also reconfigure the system to meet packaging and integration requirements. System functionality, repeatbility, and accuracy was validated by collecting data during multiple flights of a radio-controlled aircraft. Future work includes sensor fusion, thrust prediction methods, stability and control derivative estimation, and growing Cal Poly's small-scale component aerodynamic database.

This thesis is focused on conceptual design of electric powered training aircraft, using certification guidelines CS-LSA. Designing process of geometrical and aerodynamic characteristics is primary based on statistics of certificated aircrafts in this particular category. The following calculation of performance stabilities and flight performances is based on knowledge of aerodynamic polar. The calculation of the polar is also part of this thesis. The last part of the thesis consists of construction design of the front part of fuselage and engine static structural analysis of engine mounts.

京都大学 / 0048 / 新制・課程博士 / 博士(工学) / 甲第22845号 / 工博第4785号 / 新制||工||1748(附属図書館) / 京都大学大学院工学研究科物質エネルギー化学専攻 / (主査)教授 陰山 洋, 教授 藤田 晃司, 教授 阿部 竜 / 学位規則第4条第1項該当 / Doctor of Philosophy (Engineering) / Kyoto University / DGAM

No description available.