This diploma thesis deals with the optimization of microclimate of an industrial building. There is required constant temperature with minimal fluctuations during manufacturing process in the building. Due to insufficient cooling power of air-conditioning system was objective to design a solution that ensures stability of internal temperature during production especially in summer and simultaneously should be the solution economically acceptable. Simulation shows original state defined problems. Some new or unusual materials for solar shading are reviewed in the thesis by the experimental measurements and by the comparative method. To each material is assigned shading coefficient according to Czech standards, thereby is achieved universality for calculations of heat gains resulting from solar radiation. By one part of the experiments and their evaluation is determined thickness of the standard single glass glazing. It is also determined proportional transmittance of solar radiation for certain multiwall polycarbonates, which are currently used as fillers of industrial skylights and production hall‘s window openings. From experiments resulting design is subjected to the simulation. Comparison of temperatures is shown for the time before the installation of designed solar shading and after the installation of the solar shading.

The objective of the diploma thesis is the new building of Cancer Prevention Centre, first of its kind in the Czech Republic. The site is located on steep terrain of the south hillside of Žlutý kopec in Brno, which is a part of Masaryk Oncological Hospital complex. The aim of the Cancer Prevention Centre is to attract the citizens’ attention and invite them to undergo a preventive medical check-up with the potential to save hundreds of lives every year. The project takes this fact in consideration and strives to find ways leading not only towards establishing new relationship between the building and the public, but also towards connecting the city centre and nearby by residential area with the hospital complex. The three volumes of the buildings create a dynamic composition and therefore complete the characteristic development of Masaryk Oncological Centre. The main concept however revolves around the motive of instability, balance and journey which are all linked to tumour diseases.

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<p>Microbiological contamination from bacteria such as <em>Escherichia coli</em> and Salmonella is one of the main reasons for waterborne illness. Real time and accurate monitoring of water is needed in order to alleviate this human health concern. Performing multiple and parallel analysis of biomarkers such as DNA and mRNA that targets different regions of pathogen functionality provides a complete picture of its presence and viability in the shortest possible time. These biomarkers are present inside the cell and need to be extracted for analysis and detection. Hence, lysis of these pathogenic bacteria is an important part in the sample preparation for rapid detection. In addition, collecting a small amount of bacteria present in a large volume of sample and concentrating them before lysing is important as it facilitates the downstream assay. Various techniques, categorized as mechanical, chemical, thermal and electrical, have been used for lysing cells. In the electrical method, cells are lysed by exposure to an external electric field. The advantage of this method, in contrast to other methods, is that it allows lysis without the introduction of any chemical and biological reagents and permits rapid recovery of intercellular organelles. Despite the advantages, issues such as high voltage requirement, bubble generation and Joule heating are associated with the electrical method.</p> <p>To alleviate the issues associated with electrical lysis, a new design and associated fabrication process for a microfluidic cell lysis device is described in this thesis. The device consists of a nanoporous polycarbonate (PCTE) membrane sandwiched between two PDMS microchannels with electrodes embedded at the reservoirs of the microchannels. Microcontact printing is used to attach this PCTE membrane with PDMS.</p> <p>By using this PCTE membrane, it was possible to intensify the electric field at the interface of two channels while maintaining it low in the other sections of the device. Furthermore, the device also allowed electrophoretic trapping of cells before lysis at a lower applied potential. For instance, it could trap bacteria such as <em>E. coli</em> from a continuous flow into the intersection between two channels for lower electric field (308 V/cm) and lyse the cell when electric field was increased more than 1000 V/cm into that section.</p> <p>Application of lower DC voltage with pressure driven flow alleviated adverse effect from Joule heating. Moreover, gas evolution and bubble generation was not observed during the operation of this device.</p> <p>Accumulation and lysis of bacteria were studied under a fluorescence microscope and quantified by using intensity measurement. To observe the accumulation and lysis, LIVE/DEAD BacLight Bacterial Viability Kit consisting of two separate components of SYTO 9 and propidium iodide (PI) into the cell suspension in addition to GFP expressed <em>E. coli</em> were used. Finally, plate counting was done to determine the efficiency of the device and it was observed that the device could lyse 90% of bacteria for an operation voltage of 300V within 3 min.</p> <p>In conclusion, a robust, reliable and flexible microfluidic cell lysis device was proposed and analyzed which is useful for sample pretreatment in a Micro Total Analysis System.</p> / Master of Applied Science (MASc)

This master’s thesis was conducted at Linköping University in collaboration with ToyotaMaterial Handling in Mjölby to explore potential applications of recycled plastic in forklifttrucks. The primary objective was to identify the most suitable recycled plastic to be usedon the top cover of the BT Levio LWI160, which served as the focal point of this project.This was done in order to address the research questions: "What types of mechanical- andindustrial design requirements are affected when using recycled plastic (of various composi-tions), and to which new requirements and changes will result from this?" and "What are themajor mechanical- and industrial design challenges, advantages &amp; disadvantages of switchingto recycled plastic material, considering economic and sustainability factors." The study concludes that the requirements of the top cover will largely remain unchanged.The use of PP 20GF necessitates further testing for structural integrity and quality con-sistency in recycled material. This material’s harder, less ductile nature may require newimpact testing and recycling processes. Industrial design benefits include simplified colourchoices due to the material’s transparent base hue, although the dark brown colour of re-cycled plastics limits options. Ensuring consistency across batches, minimising costs, andaddressing surface defects and purity issues are critical challenges. Despite uncertainties,switching to recycled plastic can reduce CO2 emissions by up to 24% and attract environ-mentally conscious customers

The theoretical part is focused on evaluation of chosen ?essential? plastics used in the car industry. The principles of construction and proportioning of plastic components, basic conception of injection moulding and measuration follow. Another section of the theoretical part presents a summary of possible CAD systems, which are used. The practical part is focused on a particular plastic moulding. It includes the main description of a component with its material choice in dependence on the component function. Another section contains evaluation of the component complexity from the viewpoint of moulding, and a possible substitution of constructions, which could be productively simpler, is shown. The whole thesis is completed for better lucidity by pictures, drawings, and mechanical drawings.

A specialized project of „Architectural study of modification of the bicycle stadium Favorit Brno in the BVV area and urban design of the Hněvkovský sports complex in Komárov“ preceded the diploma thesis. The aim of the project was preparation of the area for cycling stadium with all the necessary facilities and functional connections. The diploma thesis develops a previous project and elaborates the compound of the cycling stadium in a form of a study. The building should recpect to the current demands of this sport, such as a closed roof of the building, siberian pine track material and a 250-meter track length. The building will be situated in the area adjacent to the current biker complex in Brno Komárov.

The content of this master’s thesis are methods of pretreatment and coating of the surface of PET to produce conductive copper structure and quality control. Thesis also includes theoretical analysis of these methods. Physical and chemical techniques of surface pretreatment methods are discussed in the theoretical part as well as methods making surface of substrate conductive, the subsequent galvanic copper plating and quality control of coating and testing of the adhesion between layers. The experimental part focuses on two methods of the polymer material surface pretreatments. The properties of these pretreatments were evaluated by using the atomic force microscopy and detection of surface energy by wetting and contact angle measurements. The surface is making conductive with cathode sputtering and electrochemical coating of copper. Adhesion of layers is tested mainly with scratch test and other methods. The results of these sub-operations are used for the realization of multi-layer conductive structures.

The master thesis covers several issues linked to a substitution of headlamp screw fixation by a plastic nail. The thesis includes an analysis of the headlamp technology, including description of the prescribed durability tests. On the basis of the analysis some new designs of plastic nails are proposed, consistent with characteristics of plastic materials. Selected outcomes of the analysis have been tested as prototypes and verified with Finite Elements Method in SimDesigner including 3D modeling capabilities provided by the Catia V5. The feasibility study regarding a casting form of the new design of plastic nails and technical economic valuation for contingent assembly mounting is included in the thesis.

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