The presented diploma thesis is focused on the study of composition and biological effects of some super-foods. Theoretical part deals with basic information about chosen superfoods and their bioactive substances. Further, theoretical part describes the overview of vesicular systems used for encapsulation and the most common methods of particle characterization. A brief review of cell cultures and cultivation of human cells is presented as well as methods for cytotoxicity a genotoxicity testing. In the experimental section, aqueous and ethanol extracts of super-foods were prepared. These extracts were then encapsulated into liposomal and combined PHB particles. Super-food extracts were characterized by spectrophotometrical methods in order to determine the content of polyphenols, flavonoids, anthocyanins, carotenes, chlorophyll, tannins, and antioxidant activity. The physico-chemical characteristics of prepared liposomal and combined particles were determined too. The particles with encapsulated extracts were further tested using the MTT assay and SOS chromotest to describe their potential cytotoxic and genotoxic effects.

The goal of this diploma thesis is to propose a system based on blockchain technology that is dealing with dual food quality problem in countries of the EU. The theore- tical part of the thesis explains basic principles of blockchain technology, provides an analysis of the dual food quality problem, EU market and the current blockchain implementations in food industry. The practical part focuses on the solution design of such a system itself.

The aim of the work was the selective isolation of bacterial DNA with help of magnetic carriers covered by streptavidine (PGMA-NH2-STV, MPG® Streptavidin). Conditions of functionalisation of carriers using two biotinylated probes were optimized: the amount of carrier, the amount of probe, binding of biotinyled probe to streptavidine. Purified DNA Lactobacillus was used for hybridization. DNA binding to the probe (DNA/DNA hybridization) and nospecific adsorption of DNA to the carrier were tested. Target DNA eluted from the carrier was identified using PCR with primers R16-1 and LbLMA1-rev and with primers P_eub and F_eub. The amount of probe bound to the carrier was estimated using UV spectrophotometry. It was estimated that biotinyled probe can be used for functionalisation in concentration 5 pmol/µl added to the carrier in the ration carrier : probe 1:1. It was shown that nonspecific DNA adsorption to the MPG® Streptavidin is significantly lower than to the carrier PGMA-NH2-STV.Using DNA/DNA hybridization and the MPG® Streptavidin, DNA from pure culture Lactobacillus was isolated. Procedure was applicated for DNA isolation from milk products.

This diploma thesis is focused on the problematic of assessment of allergenic aroma compounds in various matrices. In the frame of European legislation 26 of potentially allergenic compounds are stated, whose content in cosmetic products is limited. In the case of foods there is not such restriction so far. In the experimental part, the allergenic aroma compounds in chosen samples of cosmetic and food samples were identified and quantified using Solid phase microextraction in conjunction with gas chromatography. Sensory evaluation of aroma or taste is also included.

The aim of this work was to determine chemical species of arsenic in sea originating certified reference materials. Measurements were done using method of hydride generation. Hydrides were preconcentrated in liquid nitrogen cooled tube and detected using inductively coupled plasma mass spectrometry. During the testing of the method, mutual conversion of arsenic species was observed for inorganic arsenic, monomethyl arsenic and dimethylarsenic. Further study revealed that with presence of matrix and hydrogen peroxide, demethylation occured only in hydride generation phase, not during extraction. Dissociation of hydrogen peroxide in extracts was verified using catalase. End of the demethylation process was proved using measurement with addition of arsenic species standards. The newly invented procedure was used to determine arsenic species in reference materials with sufficient accuracy. Final results are compatible with results from different method, and already published results as well. Key words: speciation analysis, arsenic, hydride generation, seafood, inductively coupled plasma mass spectrometry

This thesis deals with functional foods and their importance in nutrition of inhabitants. The aim of thesis is to characterize the specifics of the functional foods market, demand shaping and to map the availability of a particular functional food, which is important for the health and quality of life of the inhabitants of Brno. The theoretical part defines the concept of functional food, the importance and types of functional food, including the local and world market. The practical part describes the distribution channels of the city of Brno and evaluates the primary research of the availability and prices of a particular functional food.

Diploma thesis deals with new technologies, which could be used in the ice cream manufacture. First three theoretical parts are about casual ingredients and ice cream manufacture process. Last two parts are dedicated to food with beneficial bacteria and how it influences human body. The practical part is devoted to ice cream manufacture from different milk based and plant based ice cream mixes with carob addition as a stabilizer and probiotics bacteria, which were incorporated to the mixes in different times of activations. Sensory evaluation proved, that replacement of milk has positive effect to the ice cream just as the probiotic incorporation, which causes pleasant yoghurt taste. Microbiological analysis proved sufficient content of living bacteria and therefore a benefical impact on human health.

Kramářová, D. Purchasing behaviour of visually impaired consumers. Diploma thesis. Brno: Mendel University, 2019. The diploma thesis is focused on identifying the biggest obstacles and barriers faced by visually impaired consumers when buying food. The aim is to create general recommendations for vendors based on primary research results. In-depth interviews were conducted with adult consumers (n = 30) and their observations in grocery stores when buying food (n = 5). Subsequently, managers of selected stores (n = 6) were invited to express their views approach to the issue. Field research on grocery stores was chosen as a complementary method. The primary survey was preceded by a relatively extensive orientation analysis in which mainly foreign scientific knowledge was used.

1 ABSTRACT Lorencová Š.: Nutraceuticals: possibilities in decreasing of hyperuricemia and alleviating gout. Diploma thesis, Charles University in Prague, Faculty of Pharmacy in Hradec Kralove, Department of Pharmaceutical Botany and Ecology, Hradec Kralove 2020, 82 p. This research work was conducted on the basis of literature analysis, reviewing papers mainly from international but also domestic journals. The review describes pathophysiology of gout and discusses options to mitigate the disease with the aid of dietary supplements. This work presents symptoms and a clinical picture of the disease as well as its origin and risk factors, pathological processes leading to the development of the disease, and a short overview of current pharmacotherapy. Furthermore this work summarises natural substances including plant extracts that may be utilised in the prevention and support of gout treatment. In particular, these include vitamins, unsaturated fatty acids, polyphenols and peptides. This work also describes plants used in the traditional treatment of gout and gives a brief overview of natural substances including plant extracts contained in food supplements that are available on the market in Czech Republic. This work also describes the role of purines, fructose, alcoholic drinks and tomatoes in triggering...

The topic of this diploma thesis is “Franchising in the Czech food market”. The objective of this thesis is to create a model of entry the food market in form of recommendations for future franchisees. These recommendations will be based on own work of the author, who will use strategic management methods to evaluate the market both internally and externally using methods such as SWOT, PESTE, IFE and EFE matrix or Porter’s five forces. Market analysis will be accompanied by analysis of primary data obtained from owners of active food stores who concentrate on high-quality, farmers’ and regional products. Regression analysis is used to process these data and provide results on which factors are important and which influence the economic performance of the food store. Advantages and disadvantages of franchising as seen by experienced franchisees are then presented to complete all necessary information to give final recommendation for potential franchisees for their future entrance to the market.