All life on earth depends mainly on the presence of oxygen. Largest producers of oxygen are green plants, cyanobacteria and algae. Oxygen is released from the oxygenevolving complex of photosystem II during photosynthesis and it is used in cellular respiration of all life complexes. The oxygen-evolving complex of photosystem II has the same function in each photosynthetic organism, but it has a different composition and organization of extrinsic proteins; only PsbO protein is ubiquitous in all known oxyphototrophs. Until now only low resolution electron microscopy structural models of plant PSII and crystal structures of cyanobacterial PSII are available. Higher plant extrinsic proteins (PsbP, PsbQ and PsbR) are structurally unrelated, non-homologues to the cyanobacterial extrinsic proteins (PsbO, PsbU and PsbV) and this is the reason why it is not possible to predict arrangement of these proteins on the lumenal site of higher plant PSII. Recently, models differ mainly in the structure of the oxygen-evolving complex, which could be resolved by determination of the exact binding sites for extrinsic proteins. An other question evolves: if the difference in the oxygen-evolving complex composition is the result of evolution or adaptation of photosynthetic organisms to their environment. Structural knowledge of extrinsic proteins that could help to resolve the location and subsequently the function of extrinsic proteins is still incomplete. From this case,structural analysis, interactions and probably arrangement of proteins PsbP and PsbQ was studied and is described in detail in this thesis.

Disertační práce se věnuje využití umělých neuronových sítí pro modelování elektromagnetických polí uvnitř automobilů. První část práce je zaměřena na analytický popis šíření elektromagnetických vlny interiérem pomocí Nortonovy povrchové vlny. Následující část práce se věnuje praktickému měření a ověření analytických modelů. Praktická měření byla zdrojem trénovacích a verifikačních dat pro neuronové sítě. Práce se zaměřuje na kmitočtová pásma 3 až 11 GHz a 55 až 65 GHz.

Increasing demands for low heat losses and energy intensity of a building influence energy calculations. Higher demands are placed on the accuracy of the calculations. An important part of the thermal engineering calculations is the determination of the correct boundary conditions. An important input factor is primarily the indoor and outdoor environment, and temperature is the most important parameter for these types of enviromnent. It is not always the temperature of the external environment, but the environment that is adjacent to the soil or to unheated or differently heated spaces. The possibilities of modeling temperatures below the object are described in the standard ČSN EN ISO 10211. This standard specifies details for a geometric model for the numerical calculation of heat flows to assess the total heat loss of buildings or parts thereof, as well as to derive linear and point heat transfer factors. Furthermore, to calculate minimum surface temperatures to assess the risk of surface condensation and to determine the surface temperature factors. These are two different computational models. Therefore, it would be appropriate to simplify these calculations by simplifying the boundary conditions, namely to conduct an isotherm at a certain level below the terrain, which will be considered as a boundary condition, which is also based on long-term experience with "frost-free" depth. This calculation would be unambiguous, clear and simple.

The thesis deals with the application of laser beams for examination of material properties. The properties being examined are quality of surface finish and transmissivity of materials in the optical portion of the electromagnetic spectrum. A measuring device containing laser diodes as a source of laser beam and photodiodes for evaluation of examined phenomena serves for examination of both properties. Conversion of the signal to digital quantity and its further processing take place in a microcontroller. The data is then sent via a serial bus and a converter to a computer, where it may be saved in a user program and where the user can work with them later. The thesis contains results of reflectivity measurement of a set of metal samples with different surface finish. Transmissivity measurement was applied on a set of optical filters.

The master thesis dealing with problematic about durability of concrete, with use of recycled concrete and addition of silica additions. Predominant for resistance of concrete constructions is surface layer, through which aggressive gasses and liquids penetrate from surrounding environment. The most important indicator of concrete durability is ability of surface layer transmissions of aggressive substants. In experimental part will be defined the actual state of surface layer by normal testing methods. The transmissions of surface layer will be tested on concrete cubes. Tests for transmissions of air (method TORRENT), of water (method ISAT), of acid gases (dept of carbonation by quick test in 98% CO2) will be carried out and also strength tests. Assessment of positive or negative influence of additions and amount of cement on durability and mechanical properties of concrete will be discussed in conclusion.

This work is based on the occurrence of drug residues in the environment. This study is focused on the development and optimization methods for determination of selected drugs in the surface water, aquatic sediment and sewage sludge from waste water treatment plant. From the group of drugs were chosen antibiotics. Antibiotics presented in the environment can cause adverse effects including toxic effects, immunity disorders and indirect bioalteration effects. Sulfonamide antibiotics, which are used in the treatment of urinary and respiratory tract infections as well as in the treatment of other infectious diseases, were chosen as a target compounds. Three optimized analytical methods for determination of sulfonamide antibiotics were developed. For the optimization of extraction were tested: solid phase extraction, pressurized solvent extraction, microwave extraction and ultrasonic extraction. For the final analysis was used liquid chromatography with two detectors - diode array detector and mass spectrometer. These optimized methods were applied for the analysis of real samples. The surface water and sediment samples were collected from two Moravian rivers (the Svratka river and the Svitava river). Samples of sewage sludge were collected from waste water treatment plant Brno-Modřice. Fish samples from the Svratka river were also collected. It was observed that all the selected sulfonamide antibiotics are present in real sediment samples (ug.kg-1). Simultaneously presence of some target analytes in real surface water (ug.l-1) and in sewage sludge (ug.kg-1) samples has been confirmed. In samples of surface water from the Svitava river and in fish samples sulfonamide antibiotics were not detected or their concentrations were below the limit of detection.

This work is based on the current issue of increasing concentrations of pharmaceutical residues in various components of the environment. These new environmental contaminants continuously enter the environment. The most affected environmental component is the aquatic environment. This study is focused on the development and optimization of reliable analytical method, which can determine selected drugs in the aquatic environment (waste waters and surface waters) qualitatively and quantitatively. The target compounds were selected mainly from the group of non-steroidal anti-inflammatory drugs (NSAID): salicylic acid, acetylsalicylic acid, clofibric acid, ibuprofen, acetaminophen, caffeine, naproxen, mefenamic acid, ketoprofen and diclofenac. NSAIDs are one of the most used drugs in Czech Republic. For the final analysis of this study was used the comprehensive two-dimensional gas chromatography with mass spectrometric detection Time-of-Flight (GCxGC-TOF MS). It is a very sensitive and reliable analytical method for trace and ultra-trace analysis. Simultaneously, solid phase extraction (SPE) and derivatization are optimized in this work. Optimized analytical method including SPE, derivatization with MSTFA (N-methyl-N-(trimethylsilyl) trifluoroacetamide) and final analysis by GCxGC-TOF MS were applied successfully for the analysis of real samples. Samples of waste water were collected from the waste water treatment plant in Brno – Modřice and samples of surface water were collected from two river streams Svratka and Svitava in Moravian region. The range of concentrations of selected drug residues varied from one to tens of g/L in wastewater and from tens to hundreds of ng/L in surface waters. The degree of wastewater and surface waters contamination by drug residues is assessed in conclusions of the work. Simultaneously, spontaneous degradation of selected drugs in water and removal efficiency of the wastewater treatment plant is assessed in conclusions of the work. The developed method is usable for monitoring and environmental analysis of water ecosystems. It is usable not only for selected drugs, but even for other organic compounds with similar properties.

In the presented PhD. thesis, problems covering occurrence of polybrominated diphenyl ethers (PBDE) and perfluorinated compounds in the environment were solved. The study was focused on identification and further verification of optimal methods in order to determine PBDEs and perfluorinated compounds sampled from the aquatic ecosystem. Polybrominated diphenyl ethers belong to persistent compounds classified as main pollutants; within the environmental constituents, they have been observed particularly in the last decade. For these studies, there were selected surface water matrices and sediments taken in various localities within the river Svratka basin. The analyses were to demonstrate whether they accumulate and remain in these specific matrices for longer time. There we assessed the congeners of polybrominated diphenyl ethers as follows: BDE-28, 47, 66, 85, 99, 100, 153, 154 and 183. In order to isolate them from matrices, various extraction techniques were used, i.e., ultrasonic extraction, microwave extraction, and pressure solvent extraction. Gas chromatography method with electron capture detection (GC/ECD) was selected for determination. The accomplished studies also assessed basic chemical, physical and environmental characteristics of diphenyl ethers in the environmental constituents. The attention was also paid to perfluorooctanoic compounds (PFOA), (PFOS) and (FOSA); physico-chemical properties of these organic pollutants were characterised and their toxicological and environmental aspects were evaluated. Usability of extraction techniques (ultrasonic extraction, pressure solvent extraction, solid phase extraction) applied to PFCs isolation from sediments samples was assessed. Identification and quantification of these analytes were performed using a high performance liquid chromatography/mass spectrometry method (HPLC/MS). After that, the optimized methods applied to real samples. Surface water and sediments were sampled within the river Svratka basin, in particular, from 19 sampling localities. PBDE occurrence was confirmed in sediments samples from the Svratka river (g.kg-1), PBDE were not detected in the surface water samples, their concentrations were below the detection limit, resp.; perfluorinated compounds were not detected in sediments and water samples at all.

Dissertation focuses on the verification of construction family houses made of concrete shell block with internal thermal insulation system. The construction technology is transferred to the Czech Republic from France. The work focuses on building envelope. The suitability of implementation is assessed on the basis of verification selected thermal technical requirements placed on building envelope. Requirements are specified by legislation in force in the ČR. The verification was performed by using software simulations and measurements on realized buildings. Critical areas of construction technology were identified on the basis software simulations and measurements on site. It was suggested solution, in which critical areas comply the thermal technical requirements. The building envelope was checked also using the thermography imaging on site. Continuous measurement of interior temperature was used to evaluate thermal comfort in the room. In the completed buildings was tested airtightness of the building envelope, which revealed other critical areas of the building envelope. Critical areas are necessary to solve with sufficient care. The economic cost of implementing each variants of exterior walls made of concrete shell block with internal thermal insulation were compared with the economic costs of implementing external walls made of burnt clay hollow blocks and made of burnt clay hollow blocks with external thermal insulation system ETICS. Subsequently, the economic return was calculated for individual variants of external walls made of concrete shell blocks with internal thermal insulation. From the environmental viewpoint was made comparison of production greenhouse gas CO2 and SO2 (cause acid rains) during the life cycle of the individual variations of exterior walls made of concrete shell blocks with system of internal thermal insulation, exterior walls made of burnt clay hollow blocks and burnt clay hollow blocks with external thermal insulation syst

This thesis describes methods of reconstruction of 3D scenes from photographs and videos using the Structure from motion approach. A new software capable of automatic reconstruction of point clouds and polygonal models from common images and videos was implemented based on these methods. The software uses variety of existing and custom solutions and clearly links them into one easily executable application. The reconstruction consists of feature point detection, pairwise matching, Bundle adjustment, stereoscopic algorithms and polygon model creation from point cloud using PCL library. Program is based on Bundler and PMVS. Poisson surface reconstruction algorithm, as well as simple triangulation and own reconstruction method based on plane segmentation were used for polygonal model creation.