Submitted by (edna.saturno@ufrpe.br) on 2016-07-19T14:31:33Z No. of bitstreams: 1 Clarissa Neuman Ramos Cesar.pdf: 675969 bytes, checksum: 5383e8c8bb49a0be0d444364ce184d26 (MD5) / Made available in DSpace on 2016-07-19T14:31:33Z (GMT). No. of bitstreams: 1 Clarissa Neuman Ramos Cesar.pdf: 675969 bytes, checksum: 5383e8c8bb49a0be0d444364ce184d26 (MD5) Previous issue date: 2013-04-29 / The use of artificial insemination (AI) accelerates the genetic improvement, prevents transmission of venereal diseases and facilitates testing of progeny besides enabling subfertile males to produce descendents. Fixed Time Artificial Insemination (FTAI) has been used successfully in which females have their estrus induced by hormonal treatment, which allows a prediction of the manifestation of estrus and ovulation and therefore the ideal time of insemination. In order, from the knowledge of time of ovulation, pre-determine the most appropriate period for the time of deposition of semen, cooled and frozen in the female reproductive tract in FTAI programs, twenty ewes had their estrus synchronized using intravaginal progesterone (Progespon ®, Coopers) for 12 days and 400 IU of equine chorionic gonadotropin [eCG (Novormon ®, Coopers)], to determine the moments leading up to ovulation. Other two hundred ewes, submitted to same protocol for synchronization of estrus, were divided into 4 groups for Artificial Insemination, with cooled semen used in groups 1 and 2 and frozen semen in groups 3 and 4. The sheep from G1 and G3 were inseminated 58 ± 1h after removal of progesterone intravaginal device (pre-ovulatory), and the sheeps from G2 and G4 were inseminated 64 ± 3h after withdrawal of progesterone intravaginal device (post-ovulatory). The results were obtained through the confirmation of pregnancy and data were subjected to statistical analysis with odds ratio and level of significance (p) less than 0.05. It having seen observed good results when used cooled and frozen semen, respectively, in the periods preceding (68% pregnancy rate) and exceeded (64% pregnancy) the 60h, respectively, after withdrawal of exogenous source of progestagen. / O uso da inseminação artificial acelera o melhoramento genético, evita a transmissão de doenças venéreas e facilita a realização de testes de progênie além de possibilitar que machos subférteis produzam filhos. Ultimamente, vem sendo utilizada com sucesso a inseminação artificial em tempo fixo (IATF), na qual as fêmeas têm o estro induzido por tratamento hormonal, o que permite uma previsão da manifestação do estro e ovulações e, consequentemente, o momento ideal de inseminação. Objetivando, a partir do conhecimento do momento da ovulação, pré-determinar o periodo mais adequado para o momento da deposição do sêmen, resfriado e congelado, no trato reprodutivo da fêmea, em programas de IATF, vinte ovelhas tiveram o estro sincronizado com uso de progestágenos intravaginais (Progespon®, Coopers), por 12 dias e 400UI de gonadotrofina coriônica equina [eCG (Novormon®, Coopers)], para determinação dos momentos que antecedem e marcam a ovulação. Outras duzentas ovelhas, submetidas ao mesmo protocolo para sincronização do estro, foram divididas em 4 grupos para Inseminação Artificial, tendo sido utilizado sêmen refrigerado nos grupos 1 e 2 e sêmen congelado nos grupos 3 e 4. As ovelhas que compunham os G1 e G3 foram inseminadas 58±1h após retirada do persário (pré-ovulatório), e as que compunham os G2 e G4 foram inseminadas 64±3h após retirada do persário (pós-ovulatório). Os resultados foram observados através da confirmação de prenhez e os dados submetidos à análise estatística pelo Odds ratio com e nivel de significância (p) inferior a 0,05. Tendo sido observado bons resultados quando utilizado sêmen refrigerado e congelado, respectivamente, nos períodos que antecederam (68% de prenhez) e ultrapassaram (64% de prenhez) as 60h, respectivamente, após retirada da fonte exógena de progestágeno.

O objetivo deste trabalho foi avaliar mediante testes in vitro as características espermáticas de partidas de sêmen bovino e o impacto sobre a fertilidade quando utilizadas em um programas de IATF. Foram realizados 4 experimentos que serão descritos em forma de artigos científicos. No artigo 1 comparam-se a qualidade do sêmen determinada por métodos subjetivos e objetivos para a análise espermática in vitro de partidas de sêmen bovino. Foram analisadas 80 partidas de sêmen de dois touros, através de análises convencionais (motilidade subjetiva, vigor e morfologia espermática), de acordo com os resultados foram estabelecidos 3 grupos: Alta qualidade (A), Boa qualidade (B) e Qualidade questionável (Q). Posteriormente foram analisados os resultados das análises objetivas usando sondas fluorescentes por microscopia de epifluorescência para determinar a integridade de membranas plasmática, acrossomal e potencial de membrana mitocondrial (%PIAIA); e usando o sistema computadorizado de avaliação da motilidade espermática (CASA), o efeito foi avaliado mediante ANOVA e Tukey 5%. Posteriormente, foram utilizadas 67 partidas para determinar a concordância em determinar a qualidade seminal de acordo com três métodos de avaliação seminal: (1) Análise subjetiva considerando a Motilidade subjetiva, vigor e morfologia espermática (2) Análise pelo CASA levando em consideração a MT, VCL, VSL e VAP e (3) Análise por sondas fluorescentes considerando a %PIAIA. Foram realizadas análises de correlação de Pearson e análise de concordância (significância do Kappa). No artigo 2 foram utilizadas 18 partidas de sêmen classificadas de acordo com o índice de fertilidade de cada touro em dois grupos: de Alta (n=9) e Baixa fertilidade (n=9). A classificação do escore foi proporcionada pela central com base em dados provenientes de 33.198 serviços por IATF. As partidas foram submetidas às análises convencionais, CASA, análise por sondas fluorescentes para avaliar a porcentagem de PIAIA em microscopia de epifluorescência e produção de marcadores mitocondriais por sondas fluorescentes na citometria de fluxo. Foram realizadas análises de correlação de Pearson todas as características espermáticas e os efeitos entre grupos de fertilidade foram avaliados mediante ANOVA e Tukey 5%. No artigo 3 o objetivo determinar a relação entre qualidade espermática medida pela produção de marcadores mitocondriais e integridade das estruturas espermáticas com a taxa de prenhez (TP%) resultante de um programa de inseminação artificial e tempo fixo (IATF). Neste estudo, 29 partidas de sêmen convencional usadas para inseminar 4.795 vacas da raça Nelore submetidas ao mesmo protocolo de IATF, e após o diagnóstico de prenhez foram classificadas em três grupos: Alta fertilidade (A) com TP ≥60%, Média fertilidade (M) TP entre 53,0 e 59,9% e de Baixa fertilidade (B) TP <52,2%. Doses de sêmen das mesmas partidas foram descongeladas a 37°C durante 30 segundos e submetidas às análises convencionais, CASA, análise por sondas fluorescentes para avaliar a porcentagem de PIAIA em microscopia de epifluorescência e produção de marcadores mitocondriais por sondas fluorescentes na citometria de fluxo. Foram realizadas análises de correlação de Spearman para todas as características espermáticas e os efeitos entre grupos de fertilidade foram avaliados mediante ANOVA e Tukey 5%. No artigo 4 o objetivo foi determinar as subpopulações pela morfometria espermática em partidas de sêmen com diferente escore de fertilidade. Foram utilizadas 13 partidas de sêmen de 6 touros, sendo três touros de alto escore de fertilidade (n=9) e três de baixo escore de fertilidade (n=9). Duas palhetas de cada partida foram descongeladas e uma alíquota foi depositada em formol salino (4%) e posteriormente foi feito a análise por gota húmida para aquisição de 200 imagens da cabeça espermática que foram processadas pelo programa Imagem J para determinação da morfometria, os dados foram submetidos a análise multivariada de agrupamento para formação de subpopulações pelo método de Wards e posteriormente o efeito do grupo de fertilidade foi avaliado por ANOVA e Tukey 5%. Os resultados mostraram que os marcadores da função mitocondrial são bons indicadores da função e qualidade espermática; no entanto a heterogeneidade do sêmen bovino, com subpopulações espermáticas com boa e má qualidade, varia entre touros e entre partidas do mesmo touro e gera confusão na resposta de algumas análises. Determinou-se também, que a presença de subpopulações espermáticas (SBP) com variação na morfometria da cabeça (SBP1, SBP2, SBP3 e SBP4), sendo que a SBP4 foi associada com baixo escore de fertilidade. A conclusão geral é que a qualidade seminal impacta na fertilidade in vivo em bovinos. Sendo que a morfometria da cabeça espermática e funcionalidade das mitocôndrias teve maior impacto. No entanto, todas as características de qualidade espermática devem ser avaliadas em conjunto para determinar o potencial de fertilidade de uma amostra seminal. / The objective of this study was to evaluate the sperm characteristics of bovine semen and the impact on fertility when used in an IATF program. Four experiments were carried out and described in the form of scientific articles. In article 1. We compared the semen quality determined by subjective and objective methods for the in vitro sperm analysis of bovine semen. According to the results, three groups were analyzed: high quality (A), good quality (B) and high quality (A), Questionable quality (Q). Subsequently, the results of objective analyzes using fluorescence probes by epifluorescence microscopy were analyzed to determine the integrity of plasma, acrosomal membrane and mitochondrial membrane potential (% PIAIA); And using the computerized sperm motility evaluation system (CASA), the effect was evaluated using ANOVA and Tukey 5%. Afterwards, 67 matches were used to determine the agreement to determine seminal quality according to three methods of seminal evaluation: (1) Subjective analysis considering subjective motility, vigor and sperm morphology (2) Analysis by CASA taking into consideration the TM, VCL, VSL and VAP and (3) Analysis by fluorescent probes considering % PIAIA. Pearson correlation analysis and concordance analysis (Kappa significance) were performed. In article 2, 18 sets of semen were classified according to the fertility index of each bull in two groups: High fertility (n = 9) and Low fertility (n = 9). The classification of the score was provided by the central office based on data from 33,198 services by IATF. The semen were subjected to conventional analyzes, CASA, fluorescent probe analysis to evaluate the percentage of PIAIA in epifluorescence microscopy and production of mitochondrial markers by fluorescent probes in flow cytometry. Pearson correlation analyzes were performed on all sperm characteristics and effects between fertility groups were evaluated using ANOVA and Tukey 5%. In article 3 the objective was to determine the relationship between sperm quality measured by the production of mitochondrial markers and the integrity of the spermatic structures with the pregnancy rate (TP%) resulting from an artificial insemination and fixed time (IATF) program. In this study, 29 departures of conventional semen used to inseminate 4,795 Nelore cows submitted to the same IATF protocol, and after diagnosis of pregnancy were classified into three groups: High fertility (A) with TP ≥ 60%, Average fertility ( M) TP between 53.0 and 59.9% and Low fertility (B) TP <52.2%. Semen doses of the same departures were thawed at 37 ° C for 30 seconds and subjected to conventional, CASA, fluorescent probe analysis to evaluate the percentage of PIAIA in epifluorescence microscopy and production of mitochondrial markers by fluorescent probes in flow cytometry. Spearman correlation analyzes were performed on all sperm characteristics and effects between fertility groups were evaluated using ANOVA and Tukey 5%. In article 3, 18 sets of semen were classified according to the fertility index of each bull in two groups: High (n = 9) and Low fertility (n = 9). The classification of the score was provided by the central office based on data from 33,198 services by IATF. The matches were subjected to conventional analyzes, CASA, fluorescent probe analysis to evaluate the percentage of PIAIA in epifluorescence microscopy and production of mitochondrial markers by fluorescent probes in flow cytometry. Pearson correlation analyzes were performed on all sperm characteristics and effects between fertility groups were evaluated using ANOVA and Tukey 5%. In article 4 the objective was to determine subpopulations by sperm morphometry in semen matches with different fertility scores. Seventeen sets of six bulls were used, three bulls with a high fertility score (n = 9) and three with a low fertility score (n = 9). Two straws of each set were thawed and an aliquot was deposited in saline formaldehyde (4%) and then the wet analysis was performed to acquire 200 images of the spermatic head that were processed by the Image J program to determine morphometry, the data were submitted to a multivariate cluster analysis for clusters by the Ward\'s method and later the effect of the fertility group was evaluated by ANOVA and Tukey 5%. The results showed that markers of mitochondrial function are good indicators of sperm function and quality; However, the heterogeneity of bovine semen, with good and poor quality sperm subpopulations, varies between bulls and between straws and creates confusion in the response of some analyzes. It was also determined that the presence of sperm subpopulations (SBP) with variation in head morphometry (SBP1, SBP2, SBP3 and SBP4), and SBP4 was associated with a low fertility score. The overall conclusion is that seminal quality influences in vivo fertility in cattle. Being that the morphometry of the spermatic head and functionality of the mitochondria had greater impact. However, all sperm quality characteristics should be evaluated together to determine the fertility potential of a seminal sample.

These studies deal with lifetime profit analyses for individual cows, and using these estimates to determine the economic value of genetic changes in traits for which genetic evaluations (predicted transmitting ability, PTA) are currently available. Data were collected from six states processed by Dairy Records Management Systems (DRMS) from herds on continuous test for at least 10 yr. The purpose of the first study was to determine how well estimators of lifetime net income based on 305-d lactation yields and a 10-yr opportunity (RNI305.10) and based on complete lactation data but a 5-yr opportunity (RNIc.5) predict the estimate based on complete lactations and a 10-yr opportunity (RNIc.10). Records for 22,854 cows in Virginia herds born in 1988, 1990, and 1992 from the DRMS in Raleigh, NC were used. Each RNI was calculated using fluid (skim/fat) pricing and milk-fat-protein pricing. Regression analyses including herd and birth year were used in the model to estimate the regression of RNIc.10 on RNIc.5, and RNIc.10 on RNI305.10. The resulting regression coefficients for fluid (skim/fat) pricing were $1.53 and $1.12 explaining 67 and 97% of the variation of RNIc.10, respectively. The corresponding results for milk-fat-protein pricing were $1.52 and $1.14 explaining 68 and 96% of the variation of RNIc.10, respectively. Using RNIc.10 as the measure to estimate lifetime profit is strongly recommended over the two alternatives tested. In the second study, the economic impacts of a unit change in PTA of daughter pregnancy rate (DPR) and other PTA in the merit indexes on lifetime profit estimates of a bull's daughters were estimated to determine an economic weight for the PTADPR and other PTA in economic indexes. Records for 71,094 cows born in 1988, 1990, and 1992 from six states processed at DRMS were used: Florida [10,940 cows], Indiana [8,231 cows], North Carolina [12,280 cows], Texas [4,786 cows], Virginia [20,341 cows], and Vermont [14,516 cows]. The basic RNI function consisted of [total milk, fat, and protein income ?feed cost for production] (yield income, YI) + [net value of calves + net salvage value] (non yield income, NYI) ?rearing cost (RC) ?[(daily cost for labor, maintenance feed, supplies, and fixed expenses) x days in herd] (daily cost, DC). Some of the economic impacts of PTA described for the merit indexes were not included in the basic RNI. These were added to RNI by multiplying the respective sire PTA by the economic impact. These included -165*PTASCS (M); 33*udder composite + 15*feet and legs composite -14.86*body size composite (T); and 8.064*PTA for daughter pregnancy rate -4.80*PTA for daughter calving ease (PRCE). Each ARNI was calculated using all production records initiated prior to the cow's tenth birthday with three milk pricing systems comparable to the prices in USDA three merit indexes: fluid (skim/fat) pricing (FARNI), milk-fat-protein pricing (NMARNI), and cheese pricing (CARNI). Two levels of prices for rearing cost per day and daily cost were used for calculating FARNI, NMARNI, and CARNI. Regression analyses including herd and birth year in the model were used to estimate the simple and partial regressions of ARNI or partitioned ARNI on sire PTA. Partial regression included all PTA in Net Merit, except service sire calving ease. Ignoring other PTA, one unit increase in PTADPR increased 476.25kg of lifetime total milk or 18 days of total DIM. One unit decrease in PTASCS increased 4372.50kg of lifetime total milk. With low daily and rearing costs, each 1% change in PTADPR increased ARNI by $59.31 to $55.82 depending on the milk pricing systems. The corresponding results with high daily and rearing costs were $27.50 to $24.01. Standardized multiple regression enabled the comparison of the economic weights of this study with those of USDA. The PTA for productive life (PL) in all three USDA merit index was emphasized less than the results from this study; however, PTADPR in USDA indexes was emphasized more than this study. In this study, the economic weight of PTADPR was negative within the low daily and rearing costs, but it was positive in the high daily and rearing costs. / Master of Science

O objetivo foi avaliar, por meio de uma abordagem meta-analítica, quais os principais fatores que influenciam no desempenho produtivo e reprodutivo de novilhas e vacas primíparas de corte. Para tanto, foram utilizados, de forma agregada, dados de 3.933 novilhas e vacas primíparas de corte, provenientes de 29 estudos (dissertações e teses) e 43 experimentos. As informações relacionadas à metodologia e aos resultados de cada trabalho foram tabuladas em planilha, constituindo a sistematização dos dados. Por meio dos dados disponibilizados, três artigos foram realizados. No primeiro deles, as variáveis independentes analisadas foram: idade das novilhas (14 ou 24 meses de idade) e vacas primíparas (24 ou 36 meses de idade) no momento em que foram expostas à reprodução, tipo de pastagem (pastagens de ciclo hiberno-primaveril ou natural) e tipo racial (taurinas ou mestiças). As variáveis respostas foram os pesos e escores de condição corporal ao início e final do período reprodutivo e a taxa de prenhez. Maiores pesos corporais ao início do acasalamento e taxa de prenhez foram observados nas novilhas expostas à reprodução aos 24 meses (325,25±1,55 kg e 73,84±1,41%), nas mestiças (321,41±2,15 kg e 70,88±1,63%) e nas que pastejaram pastagens de ciclo hiberno-primaveril no pré-acasalamento (318,34±2,01 kg e 82,43±1,60%). Maiores pesos corporais foram observados nas vacas primíparas expostas à reprodução aos 36 meses de idade (376,09±1,55 kg), nas mestiças (373,56±1,54 kg) e nas que pastejaram pastagens de ciclo hiberno-primaveril (377,80±2,29 kg). A maior prenhez foi observada nas primíparas aos 24 meses de idade (83,44±3,03%, nas taurinas (88,53±2,61%) e nas que pastejaram pastagens de ciclo hiberno-primaveril (77,68±1,93%). No segundo trabalho, a taxa de prenhez foi considerada a variável resposta. Foram realizadas análises de sub-grupos entre categoria animal (novilhas e vacas primíparas), idade das fêmeas no momento em que foram expostas à reprodução (14, 24 ou 36 meses de idade), tipo de pastagem (pastagens de ciclo hiberno-primaveril ou natural) e tipo racial (taurinas ou mestiças). Novilhas e vacas primíparas, expostas à reprodução apresentaram maior taxa de prenhez (80,24%). No terceiro trabalho, a variável resposta foi a taxa de prenhez.Fatores explanatórios foram categoria animal (novilhas ou vacas primíparas), peso corporal ao início do período reprodutivo, ganho médio diário durante a reprodução, tipo racial (taurinas e mestiças) e taxa de lotação. O peso ao início da reprodução influenciou 93,1% da taxa de prenhez. Por meio da presente meta-análise, a idade à qual as fêmeas foram expostas à reprodução, a raça, o tipo de pastagem utilizada no pré-acasalamento, o peso corporal ao início da reprodução e a taxa de lotação são fatores que influenciam o desempenho produtivo e reprodutivo de novilhas e vacas primíparas de corte no sul do Brasil. / The aim was to evaluate, through a meta-analytical approach, the main factors that influence the productive and reproductive performance of heifers and primiparous cows. We used, in aggregate, data from 3,933 heifers and primiparous cows from 29 studies (dissertations and theses) and 43 experiments. The information related to the methodology and the results of each work were tabulated in a spreadsheet, constituting the systematization of the data. Through the available data, three articles were made. In the first one, the independent variables analyzed were: age of heifers (14 or 24 months of age) and primiparous cows (24 or 36 months of age) at the time they were exposed to reproduction, type of pasture (winter-spring cycle or natural pasture) and breed (Taurine or crossbred). The variables responses were the weight and body condition scores at the beginning and end of the reproductive period and the pregnancy rate. Larger body weights at the beginning of the breeding season and pregnancy rate were observed in heifers exposed to reproduction at 24 months (325.25±1.55 kg and 73.84±1.41%), in crossbreed (321.41±2,15 kg and 70.88±1.63%) and in those that grazed winter-spring cycle pastures in pre-mating (318.34±2.01 kg and 82.43±1.60%). Higher body weights were observed in the primiparous cows exposed to the breeding season at 36 months of age (376.09±1.55 kg), in the crossbreed (373.56±1.54 kg) and in those that grazed hiberno-spring cycle (377.80±2.29 kg). The highest pregnancy was observed in the primiparous cows at the 24 months of age (83.44±3.03%), in the taurine (88.53±2.61%) and in those that grazed winter-spring cycle pastures (77.68±1.93%). In the second study, the pregnancy rate was considered the response variable. Was evaluated analysis of subgroups between animal categories (heifers and primiparous cows), age of females at the time of the breeding season (14, 24 or 36 months of age), type of pasture (winter-spring cycle or natural pasture) and breed (Taurine or crossbreed). Heifers and primiparous cows, exposed to reproduction, had a higher pregnancy rate (80.24%). In the third study, the response variable was the pregnancy rate. Explanatory factors were animal category (heifers or primiparous cows), body weight at the beginning of the breeding season, average daily gain during the breeding season, breed (Taurine and crossbreed) and stocking rate. Through the present meta-analysis, the age at the which females were exposed to the breeding season, breed, type of pasture used in pre-mating, body weight at the beginning of the breeding season and stocking rate are factors that influence productive performance and reproductive performance of heifers and primiparous beef cattle in southern Brazil.

Background: Chlamydia trachomatis in women is a risk factor for tubal factor infertility and extra uterine pregnancies, but the impact of a C. trachomatis infection on male fertility is unclear. It is also hypothesized that persistent infection with C. trachomatis, or other microorganisms, might initiate/promote ovarian tumor development. The aims of the thesis were to study whether C. trachomatis serum antibodies in women and men had an impact on infertility diagnoses, semen characteristics, pregnancy rates and pregnancy outcomes; furthermore, to explore associations of C. trachomatis, and Mycoplasma genitalium, plasma antibodies with epithelial ovarian cancer and borderline ovarian tumors, as well as the presence of C. trachomatis bacteria, and other microorganisms, in ovarian tissues. Materials and methods: Papers I and II: 244/226 infertile couples were tested for serum C. trachomatis IgG, IgA, IgM and chlamydial Heat Shock Protein 60 (cHSP60) IgG antibodies. C. trachomatis IgG positive couples were also tested for C. trachomatis DNA in a urine sample. The follow-up period was 14-54 months. 244 spontaneously pregnant women were also tested for serum C. trachomatis IgG antibodies. Papers III and IV: Plasma samples from 291 women with epithelial ovarian cancer, borderline ovarian tumors and benign conditions, and plasma samples from 271 healthy controls, were analyzed for C. trachomatis IgG, IgA and cHSP60-1 IgG and M. genitalium IgG antibodies. Ovarian tissues from 186 women with benign ovaries, borderline ovarian tumors and epithelial ovarian cancer, as well as tissues from the contra lateral ovary in 126 women, were analyzed for the presence of C. trachomatis, M. genitalium, Neisseria gonorrhoeae, HPV and the polyoma viruses BKV and JCV with nucleic acid amplification tests. Results: Papers I and II: The prevalence of C. trachomatis IgG antibodies was higher among infertile than fertile women, and there were 9 couples with ongoing C. trachomatis infections. In men, C. trachomatis IgG and IgA antibodies were associated with a reduced likelihood to achieve pregnancy for the couple, as well as lower sperm concentration, reduced sperm motility and vitality, increased teratozoospermia index and the occurrence of leukocytes. C. trachomatis IgG and cHSP60 IgG antibodies in infertile women were associated with tubal factor infertility, but not with reduced pregnancy rates or outcomes. Paper III: cHSP60-1 IgG antibodies were associated with ovarian cancer belonging to the postulated type II pathogenetic pathway when plasma samples obtained more than one year prior to diagnosis were analyzed. M. genitalium IgG antibodies were associated with borderline ovarian tumors; however a statistical type 1 error cannot be excluded. Paper IV: None of the microorganisms studied were found in the ovarian tissue samples. Conclusions: C. trachomatis IgG and IgA antibodies in the man substantially decreases the chances of the infertile couple to achieve pregnancy, and are associated with subtle negative changes in semen characteristics. C. trachomatis IgG and cHSP60 IgG antibodies in the woman are risk factors for tubal factor infertility. Prospective plasma cHSP60-1 IgG antibodies are associated with type II ovarian carcinomas, but C. trachomatis bacteria, or the other microorganisms studied, could not be detected in benign, borderline or malignant ovarian tissues.

Este trabalho propõe uma metodologia para auxiliar o empresário rural no momento de decidir qual a tecnologia menos arriscada para aumentar a taxa de prenhez em seu sistema de produção de bezerros. Para tanto, foram avaliadas as percepções de especialistas quanto a sete fatores a partir dos quais foi desenvolvida uma equação para calcular este risco. Tais parâmetros e suas definições foram apresentados em questionários aos 18 especialistas, selecionados por amostragem não probabilística. Neste documento foram listadas 32 tecnologias, avaliadas de acordo com os parâmetros conforme a escala Likert de cinco níveis. Foram utilizados os parâmetros relacionados diretamente ao risco das tecnologias, atribuindo-se um valor conforme a nota dos especialistas, de forma a ajusta-las conforme sua influência no risco, se positiva ou negativa. Não houve diferença significativa entre o risco calculado e o estimado pelos especialistas, além de ambos apresentarem alta correlação, validando esta metodologia. As tecnologias de insumo se apresentaram mais arriscadas, indicando custo, complexidade operacional e conhecimento técnico mais elevados do que as de processo. Da mesma forma, as tecnologias de manejo demonstraram menor risco em relação às nutricionais e reprodutivas, que não apresentaram diferença entre si. As tecnologias consideradas inovadoras foram mais arriscadas do que as consolidadas, apresentando todos os parâmetros mais elevados, com exceção da flexibilidade. Dessa forma, a metodologia desenvolvida foi capaz de determinar o risco das tecnologias, obtendo resultados semelhantes às percepções dos especialistas. / This study proposes a methodology to assist the farmers in deciding which technology is less risky to reduce the inefficiencies of their cow-calf production. Thus, were evaluated the perceptions of experts about seven factors predetermined from which were developed a formula to calculate this risk. These parameters and their definitions were reported in questionnaires to 18 experts, selected by nonprobability sampling. This document listed 32 technologies evaluated according to the parameters in the Likert scale of five levels. Were used the parameters directly related to the risk, assigning a value to the experts notes, in order to adjust these as their influence on the risk, whether positive or negative. There was no significant difference between the calculated and the estimated risks; they also had a high correlation, validating this methodology. The innovative technologies were more risky, indicating cost, operational complexity and technical knowledge higher than the process technologies. Likewise, the management technologies showed less risk than the nutritional and reproductive ones, which appear to have no difference among them. The innovative technologies were riskier than the consolidated ones, with all the standards higher, with the exception of flexibility. The methodology was able to determine the risk of technologies, obtaining similar results to the perceptions of the experts.

O objetivo foi avaliar, por meio de uma abordagem meta-analítica, quais os principais fatores que influenciam no desempenho produtivo e reprodutivo de novilhas e vacas primíparas de corte. Para tanto, foram utilizados, de forma agregada, dados de 3.933 novilhas e vacas primíparas de corte, provenientes de 29 estudos (dissertações e teses) e 43 experimentos. As informações relacionadas à metodologia e aos resultados de cada trabalho foram tabuladas em planilha, constituindo a sistematização dos dados. Por meio dos dados disponibilizados, três artigos foram realizados. No primeiro deles, as variáveis independentes analisadas foram: idade das novilhas (14 ou 24 meses de idade) e vacas primíparas (24 ou 36 meses de idade) no momento em que foram expostas à reprodução, tipo de pastagem (pastagens de ciclo hiberno-primaveril ou natural) e tipo racial (taurinas ou mestiças). As variáveis respostas foram os pesos e escores de condição corporal ao início e final do período reprodutivo e a taxa de prenhez. Maiores pesos corporais ao início do acasalamento e taxa de prenhez foram observados nas novilhas expostas à reprodução aos 24 meses (325,25±1,55 kg e 73,84±1,41%), nas mestiças (321,41±2,15 kg e 70,88±1,63%) e nas que pastejaram pastagens de ciclo hiberno-primaveril no pré-acasalamento (318,34±2,01 kg e 82,43±1,60%). Maiores pesos corporais foram observados nas vacas primíparas expostas à reprodução aos 36 meses de idade (376,09±1,55 kg), nas mestiças (373,56±1,54 kg) e nas que pastejaram pastagens de ciclo hiberno-primaveril (377,80±2,29 kg). A maior prenhez foi observada nas primíparas aos 24 meses de idade (83,44±3,03%, nas taurinas (88,53±2,61%) e nas que pastejaram pastagens de ciclo hiberno-primaveril (77,68±1,93%). No segundo trabalho, a taxa de prenhez foi considerada a variável resposta. Foram realizadas análises de sub-grupos entre categoria animal (novilhas e vacas primíparas), idade das fêmeas no momento em que foram expostas à reprodução (14, 24 ou 36 meses de idade), tipo de pastagem (pastagens de ciclo hiberno-primaveril ou natural) e tipo racial (taurinas ou mestiças). Novilhas e vacas primíparas, expostas à reprodução apresentaram maior taxa de prenhez (80,24%). No terceiro trabalho, a variável resposta foi a taxa de prenhez.Fatores explanatórios foram categoria animal (novilhas ou vacas primíparas), peso corporal ao início do período reprodutivo, ganho médio diário durante a reprodução, tipo racial (taurinas e mestiças) e taxa de lotação. O peso ao início da reprodução influenciou 93,1% da taxa de prenhez. Por meio da presente meta-análise, a idade à qual as fêmeas foram expostas à reprodução, a raça, o tipo de pastagem utilizada no pré-acasalamento, o peso corporal ao início da reprodução e a taxa de lotação são fatores que influenciam o desempenho produtivo e reprodutivo de novilhas e vacas primíparas de corte no sul do Brasil. / The aim was to evaluate, through a meta-analytical approach, the main factors that influence the productive and reproductive performance of heifers and primiparous cows. We used, in aggregate, data from 3,933 heifers and primiparous cows from 29 studies (dissertations and theses) and 43 experiments. The information related to the methodology and the results of each work were tabulated in a spreadsheet, constituting the systematization of the data. Through the available data, three articles were made. In the first one, the independent variables analyzed were: age of heifers (14 or 24 months of age) and primiparous cows (24 or 36 months of age) at the time they were exposed to reproduction, type of pasture (winter-spring cycle or natural pasture) and breed (Taurine or crossbred). The variables responses were the weight and body condition scores at the beginning and end of the reproductive period and the pregnancy rate. Larger body weights at the beginning of the breeding season and pregnancy rate were observed in heifers exposed to reproduction at 24 months (325.25±1.55 kg and 73.84±1.41%), in crossbreed (321.41±2,15 kg and 70.88±1.63%) and in those that grazed winter-spring cycle pastures in pre-mating (318.34±2.01 kg and 82.43±1.60%). Higher body weights were observed in the primiparous cows exposed to the breeding season at 36 months of age (376.09±1.55 kg), in the crossbreed (373.56±1.54 kg) and in those that grazed hiberno-spring cycle (377.80±2.29 kg). The highest pregnancy was observed in the primiparous cows at the 24 months of age (83.44±3.03%), in the taurine (88.53±2.61%) and in those that grazed winter-spring cycle pastures (77.68±1.93%). In the second study, the pregnancy rate was considered the response variable. Was evaluated analysis of subgroups between animal categories (heifers and primiparous cows), age of females at the time of the breeding season (14, 24 or 36 months of age), type of pasture (winter-spring cycle or natural pasture) and breed (Taurine or crossbreed). Heifers and primiparous cows, exposed to reproduction, had a higher pregnancy rate (80.24%). In the third study, the response variable was the pregnancy rate. Explanatory factors were animal category (heifers or primiparous cows), body weight at the beginning of the breeding season, average daily gain during the breeding season, breed (Taurine and crossbreed) and stocking rate. Through the present meta-analysis, the age at the which females were exposed to the breeding season, breed, type of pasture used in pre-mating, body weight at the beginning of the breeding season and stocking rate are factors that influence productive performance and reproductive performance of heifers and primiparous beef cattle in southern Brazil.

Made available in DSpace on 2015-03-26T13:47:09Z (GMT). No. of bitstreams: 1 texto completo.pdf: 269793 bytes, checksum: 7ace1f379fb4f111e4dbe56c3e1179e0 (MD5) Previous issue date: 2006-12-18 / Fundação de Amparo a Pesquisa do Estado de Minas Gerais / The objective of the present study was to evaluate the effect of the administration, of 500 mg rbST (recombinant bovine somatotropin) in the moment of estrous of recipient heifers and cows inovulation with fresh embryos and 250 and 500 mg rbST in recipient inovulated with defrosted embryos, in progesterone serum concentration (P4) on the day of the inovulation and at the pregnancy rate at day 30. Blood samples were collected in the day of the inovulation by puncture of the coccygeal vein or artery for assaying of the serum P4 concentration. 259 crossbred recipients, appraised as able for reproduction, were used and divided in two studies. In the first study 144 recipient heifers received fresh embryos, randomly sorted among the experiments. The control (T1) was composed by 77 recipients, and the treated (T2) 67 recipients that received 500mg rbST at the moment of estrous. In a second study, it was used 115 recipients that receive defrosted embryos subdivided in 3 treatments. 53 recipient heifers composed the control (T1), the treated 2 (T2) was composed by 22 recipients that received 250 mg of rbST and the treated 3 (T3) was composed by 40 recipients that received 500 mg of rbST. The pregnancy rate of the first study was 65.7% (44 pregnant recipients) among the animals of T2 and 32.5% (25 pregnant recipients) at T1, which demonstrates the positive effect (P<0.01) from the use of rbST on pregnancy rate. However, the average concentrations of serum P4 in the day of the inovulation was 2.54±0.19 mg/ml for the animals of T1 and 2.27±0.20 mg/ml for the T2, there was no difference (P>0.05). The pregnancy rate of the second study recipients was 45.3% (24 recipients) among the animals of the group T1, 50.0% (11 recipient) for T2 and 52.5% (21 recipient) for T3. The average concentrations of serum P4 in the day of the inovulation were: T1 (control) = 5.41±2.33 mg/ml, T2 (250 mg rbST) = 5.77±2.19 mg/ml and T3 (treated - 500 mg rbST) = 4.77 ± 1.78 mg/ml. The results of the second study demonstrate the absence of effect (P>0.05) on the rbST use over the pregnancy rate and the serum P4 concentration. We can conclude that the administration of 500 mg rbST in recipient inovulation that receive fresh embryo at the moment of the estrous was able to improve the pregnancy rate, but not the concentration of progesterone serum P4. However, when administered 250 or 500 mg rbST in recipient inovulated that receive defrosted embryos, we are able to conclude that there was no positive effect on the pregnancy rate and on the progesterone serum concentration. / O objetivo do presente estudo foi avaliar o efeito da administração, no dia do estro, de 500 mg de rbST (somatotropina bovina recombinante) em receptoras inovuladas com embriões transferidos à fresco e de 250 e 500 mg de rbST em receptoras inovuladas com embriões descongelados, sobre a concentração sérica de progesterona (P4) no dia da inovulação e a taxa de gestação aos 30 dias. As amostras de sangue foram coletadas no dia das inovulações por punção da veia ou artéria coccígea para análise da concentração sérica de P4. Foram utilizadas 259 receptoras mestiças, avaliadas como aptas a reprodução e divididas em dois estudos. No primeiro estudo foram utilizadas 144 receptoras, inovuladas com embriões à fresco, distribuídas aleatoriamente entre os tratamentos. O controle (T1) foi constituído por 77 receptoras e o tratado (T2) por 67 receptoras que receberam a administração de 500 mg de rbST no momento do estro. Em um segundo estudo utilizou-se 115 receptoras inovuladas com embriões descongelados subdivididas em três tratamentos. O controle (T1) foi constituido por 53 receptoras, o tratado 2 (T2) por 22 receptoras submetidas a administração de 250 mg de rbST e o tratado 3 (T3) por 40 receptoras que receberam a administração de 500 mg de rbST. As fêmeas do primeiro estudo apresentaram taxas de gestações de 65,7% (44 receptoras gestantes) entre os animais do grupo T2 e 32,5% (25 receptoras gestantes) para os do grupo T1, o que demonstra o efeito positivo (P<0,01) do uso da rbST sobre a taxa de gestação. Entretanto, as concentrações médias de P4 sérica no dia da inovulação foram de 2,54±0,19 ng/mL para os animais do T1 e 2,27±0,20 ng/mL para os do T2, não sendo observada diferença (P>0,05). No segundo estudo as taxas de gestações encontradas foram de 45,3% (24 receptoras) para T1, 50% (11 receptoras) para T2 e 52,5% (21 receptoras) para T3. As concentrações médias de P4 sérica no dia da inovulação foram de T1 (controle) = 5,41±2,33 ng/mL, T2 (250 mg rbST) = 5,77±2,19 ng/mL e T3 (500 mg rbST) = 4,77±1,78 ng/mL. Os resultados obtidos neste segundo estudo demonstram a ausência de efeito (P>0,05) do uso da rbST sobre as taxas de gestações e as concentrações séricas de P4. Concluiu-se que a aplicação de 500 mg de rbST em receptoras inovuladas com embriões à fresco no momento do estro, foi capaz de melhorar a taxa de gestação, mas não a concentração sérica de progesterona. No entanto, ao serem administradas 250 ou 500 mg de rbST receptoras inovuladas com embriões descongelados conclui-se a ausência de efeito positivo nas taxas de gestações e nas concentrações séricas de progesterona.

O objetivo foi avaliar, por meio de uma abordagem meta-analítica, quais os principais fatores que influenciam no desempenho produtivo e reprodutivo de novilhas e vacas primíparas de corte. Para tanto, foram utilizados, de forma agregada, dados de 3.933 novilhas e vacas primíparas de corte, provenientes de 29 estudos (dissertações e teses) e 43 experimentos. As informações relacionadas à metodologia e aos resultados de cada trabalho foram tabuladas em planilha, constituindo a sistematização dos dados. Por meio dos dados disponibilizados, três artigos foram realizados. No primeiro deles, as variáveis independentes analisadas foram: idade das novilhas (14 ou 24 meses de idade) e vacas primíparas (24 ou 36 meses de idade) no momento em que foram expostas à reprodução, tipo de pastagem (pastagens de ciclo hiberno-primaveril ou natural) e tipo racial (taurinas ou mestiças). As variáveis respostas foram os pesos e escores de condição corporal ao início e final do período reprodutivo e a taxa de prenhez. Maiores pesos corporais ao início do acasalamento e taxa de prenhez foram observados nas novilhas expostas à reprodução aos 24 meses (325,25±1,55 kg e 73,84±1,41%), nas mestiças (321,41±2,15 kg e 70,88±1,63%) e nas que pastejaram pastagens de ciclo hiberno-primaveril no pré-acasalamento (318,34±2,01 kg e 82,43±1,60%). Maiores pesos corporais foram observados nas vacas primíparas expostas à reprodução aos 36 meses de idade (376,09±1,55 kg), nas mestiças (373,56±1,54 kg) e nas que pastejaram pastagens de ciclo hiberno-primaveril (377,80±2,29 kg). A maior prenhez foi observada nas primíparas aos 24 meses de idade (83,44±3,03%, nas taurinas (88,53±2,61%) e nas que pastejaram pastagens de ciclo hiberno-primaveril (77,68±1,93%). No segundo trabalho, a taxa de prenhez foi considerada a variável resposta. Foram realizadas análises de sub-grupos entre categoria animal (novilhas e vacas primíparas), idade das fêmeas no momento em que foram expostas à reprodução (14, 24 ou 36 meses de idade), tipo de pastagem (pastagens de ciclo hiberno-primaveril ou natural) e tipo racial (taurinas ou mestiças). Novilhas e vacas primíparas, expostas à reprodução apresentaram maior taxa de prenhez (80,24%). No terceiro trabalho, a variável resposta foi a taxa de prenhez.Fatores explanatórios foram categoria animal (novilhas ou vacas primíparas), peso corporal ao início do período reprodutivo, ganho médio diário durante a reprodução, tipo racial (taurinas e mestiças) e taxa de lotação. O peso ao início da reprodução influenciou 93,1% da taxa de prenhez. Por meio da presente meta-análise, a idade à qual as fêmeas foram expostas à reprodução, a raça, o tipo de pastagem utilizada no pré-acasalamento, o peso corporal ao início da reprodução e a taxa de lotação são fatores que influenciam o desempenho produtivo e reprodutivo de novilhas e vacas primíparas de corte no sul do Brasil. / The aim was to evaluate, through a meta-analytical approach, the main factors that influence the productive and reproductive performance of heifers and primiparous cows. We used, in aggregate, data from 3,933 heifers and primiparous cows from 29 studies (dissertations and theses) and 43 experiments. The information related to the methodology and the results of each work were tabulated in a spreadsheet, constituting the systematization of the data. Through the available data, three articles were made. In the first one, the independent variables analyzed were: age of heifers (14 or 24 months of age) and primiparous cows (24 or 36 months of age) at the time they were exposed to reproduction, type of pasture (winter-spring cycle or natural pasture) and breed (Taurine or crossbred). The variables responses were the weight and body condition scores at the beginning and end of the reproductive period and the pregnancy rate. Larger body weights at the beginning of the breeding season and pregnancy rate were observed in heifers exposed to reproduction at 24 months (325.25±1.55 kg and 73.84±1.41%), in crossbreed (321.41±2,15 kg and 70.88±1.63%) and in those that grazed winter-spring cycle pastures in pre-mating (318.34±2.01 kg and 82.43±1.60%). Higher body weights were observed in the primiparous cows exposed to the breeding season at 36 months of age (376.09±1.55 kg), in the crossbreed (373.56±1.54 kg) and in those that grazed hiberno-spring cycle (377.80±2.29 kg). The highest pregnancy was observed in the primiparous cows at the 24 months of age (83.44±3.03%), in the taurine (88.53±2.61%) and in those that grazed winter-spring cycle pastures (77.68±1.93%). In the second study, the pregnancy rate was considered the response variable. Was evaluated analysis of subgroups between animal categories (heifers and primiparous cows), age of females at the time of the breeding season (14, 24 or 36 months of age), type of pasture (winter-spring cycle or natural pasture) and breed (Taurine or crossbreed). Heifers and primiparous cows, exposed to reproduction, had a higher pregnancy rate (80.24%). In the third study, the response variable was the pregnancy rate. Explanatory factors were animal category (heifers or primiparous cows), body weight at the beginning of the breeding season, average daily gain during the breeding season, breed (Taurine and crossbreed) and stocking rate. Through the present meta-analysis, the age at the which females were exposed to the breeding season, breed, type of pasture used in pre-mating, body weight at the beginning of the breeding season and stocking rate are factors that influence productive performance and reproductive performance of heifers and primiparous beef cattle in southern Brazil.

Este trabalho propõe uma metodologia para auxiliar o empresário rural no momento de decidir qual a tecnologia menos arriscada para aumentar a taxa de prenhez em seu sistema de produção de bezerros. Para tanto, foram avaliadas as percepções de especialistas quanto a sete fatores a partir dos quais foi desenvolvida uma equação para calcular este risco. Tais parâmetros e suas definições foram apresentados em questionários aos 18 especialistas, selecionados por amostragem não probabilística. Neste documento foram listadas 32 tecnologias, avaliadas de acordo com os parâmetros conforme a escala Likert de cinco níveis. Foram utilizados os parâmetros relacionados diretamente ao risco das tecnologias, atribuindo-se um valor conforme a nota dos especialistas, de forma a ajusta-las conforme sua influência no risco, se positiva ou negativa. Não houve diferença significativa entre o risco calculado e o estimado pelos especialistas, além de ambos apresentarem alta correlação, validando esta metodologia. As tecnologias de insumo se apresentaram mais arriscadas, indicando custo, complexidade operacional e conhecimento técnico mais elevados do que as de processo. Da mesma forma, as tecnologias de manejo demonstraram menor risco em relação às nutricionais e reprodutivas, que não apresentaram diferença entre si. As tecnologias consideradas inovadoras foram mais arriscadas do que as consolidadas, apresentando todos os parâmetros mais elevados, com exceção da flexibilidade. Dessa forma, a metodologia desenvolvida foi capaz de determinar o risco das tecnologias, obtendo resultados semelhantes às percepções dos especialistas. / This study proposes a methodology to assist the farmers in deciding which technology is less risky to reduce the inefficiencies of their cow-calf production. Thus, were evaluated the perceptions of experts about seven factors predetermined from which were developed a formula to calculate this risk. These parameters and their definitions were reported in questionnaires to 18 experts, selected by nonprobability sampling. This document listed 32 technologies evaluated according to the parameters in the Likert scale of five levels. Were used the parameters directly related to the risk, assigning a value to the experts notes, in order to adjust these as their influence on the risk, whether positive or negative. There was no significant difference between the calculated and the estimated risks; they also had a high correlation, validating this methodology. The innovative technologies were more risky, indicating cost, operational complexity and technical knowledge higher than the process technologies. Likewise, the management technologies showed less risk than the nutritional and reproductive ones, which appear to have no difference among them. The innovative technologies were riskier than the consolidated ones, with all the standards higher, with the exception of flexibility. The methodology was able to determine the risk of technologies, obtaining similar results to the perceptions of the experts.