Humans impact rivers in many ways that modify ecological processes yielding ecosystem services. In order to mitigate anthropogenic impacts, scientists are challenged to understand interactions among physicochemical factors affecting large river food webs. An understanding of socioeconomic factors also is critical for ecosystem management. In this dissertation, I explore spatiotemporal patterns in floodplain river food webs and political barriers to management of environmental flows, an important factor influencing river ecology. In Chapter II, I reviewed the scientific literature to test conceptual models of river food webs and predictions of environmental factors that might produce variation in basal production sources supporting consumer biomass. My review indicates that algae are the predominant production source for large rivers worldwide, but consumers assimilate C3 plants in rivers 1) with high sediment loads and low transparency during high flow pulses, 2) with high dissolved organic matter concentrations, and 3) following periods of high discharge or leaf litter fall that increase the amount of terrestrial material in the particulate organic matter pool. In Chapter III, I descrobe field research conducted to examine relationships among hydrology, nutrient concentrations, turbidity, and algal primary production and biomass in the littoral zone of five rivers in Texas, Peru, and Venezuela differing in physicochemical conditions. I used stable isotope signatures to estimate contributions of algal-versus terrestrial-based production sources to consumers during different hydrologic periods. My research indicates that during flow pulses in floodplain rivers, a decrease in algal biomass and productivity, combined with increased inputs of terrestrial organic matter, can result in increased terrestrial support of metazoan consumers in the aquatic food web. In 2007, Texas Senate Bill 3 directed that environmental flow recommendations be developed for river basins. Despite emphasis on use of the "best available science" to develop environmental flow regimes and "stakeholder involvement" to address needs of all water users, for the first two basins to complete the SB3 process, final environmental flow rules did not mimic a natural flow regime. In Chapter IV, I reviewed this process, concluding that incentives for river authorities to increase compromise with diverse stakeholders should result in more sustainable management of freshwater.

Phytoplankton dynamics and chemical characteristics of the euphotic zone were measured from 1998-2000 (an El Niño/La Niña cycle) at the 5 major stations along Line P. Near-shelf and offshore stations exhibited low seasonality in chlorophyll and moderate seasonality in particulate organic carbon (POC) production. During the 1998 El Niño, June was characterized by low chlorophyll and POC productivity due to nitrate depletion. In contrast, during the 1999 La Niña, and in 2000, higher POC productivity and nitrate occurred in June. During 1999, chlorophyll and POC productivity were similar to 1998 in late summer. Near-shelf biomass was highest in June and lowest in Feb. for the near-shelf stations. High nitrate, low chlorophyll (HNLC) stations had the highest chlorophyll in Feb. followed by June. The coccolithophore assemblage was usually numerically dominated by Emiliania huxleyi, particularly in June. Along the transect, coccolithophore abundance was much higher in June during the 1998 El Niño than in the 1999 La Niña, with Aug./Sept. abundance of both years being very low. Higher abundances were measured along the transect in June and the late summer of 2000 with sporadic ‘blooms’ of >1000 cells ml⁻¹ at some stations. Particulate inorganic carbon (PIC) production was high along the transect during June 1998, and low during both winters, June 1999 and during late summers of 1998 and 1999. There was an increase in diatom biomass and >20 µm POC production during the 1998 El Niño, specifically in the farthest offshore HNLC stations, yet diatoms were rarely found to dominate total phytoplankton biomass or production. However, there were some sporadic examples of anomalously high diatom biomass (carbon and abundance) as well as >20 µm POC production, specifically at P12 in Aug./Sept 2000. The same major diatom species were found throughout Line P (near-shelf, P16, and HNLC). Integrated silica production measured by ³²Si ranged from 0.2 to 4.7 mmol Si m⁻² d⁻¹ between 1999-2000. Silicic acid and nitrate were never limiting at all stations in Feb. and generally increased in concentration along Line P during all seasons.

A Lagoa Mangueira é uma extensa lagoa rasa subtropical localizada no sul do estado do Rio Grande do Sul, Brasil. É um ambiente que varia de oligo à mesotrófico, com extensa área litoral colonizada por macrófitas aquáticas, fornecendo grande área de substrato para o desenvolvimento da comunidade aderida. A parte sul da lagoa é caracterizada pela alta transparência e alto pH, e a parte norte é mais rasa com alta concentração de ácidos húmicos devido a interação com banhado. Devido sua forma e posição geográfica a lagoa sofre constante ação dos ventos de direção NE, com mudanças para o quadrante SE-SO durante as frentes frias. O vento neste ambiente é um fator regulador da hidrodinâmica, afetando espacial e temporalmente as comunidades aquáticas (fitoplâncton, zooplâncton, e peixes), podendo ser considerado um distúrbio constante no ambiente. Entretanto, ainda há uma lacuna quanto à contribuição das algas epifíticas para a teia trófica, bem como a resposta dessa comunidade à hidrodinâmica. O Capítulo 1 apresenta um estudo que testou se as diatomáceas epifíticas respondem a heterogeneidade espacial da lagoa durante dois verões em três pontos distantes (Norte, Centro e Sul). Verificou-se que as diatomáceas exibem um gradiente longitudinal NS e a área central assume características semelhantes aos pontos Norte e Sul dependendo da dinâmica do vento. No Capítulo 2, foi avaliada a sucessão das algas epifíticas no Sul da lagoa, durante 60 dias no verão de 2012, para investigar os fatores reguladores do desenvolvimento da comunidade in situ, considerando o zooplâncton como potencial predador. O estudo evidenciou que a fase avançada da sucessão foi dominada por diatomáceas fortemente aderidas e adaptadas às altas médias da velocidade do vento (±15 m s-1). O epifíton mostrou-se uma grande fonte de produtividade primária para a teia trófica e fortes correlações provaram que a comunidade sustentou o controle bottom-up da comunidade zooplanctônica. O Capítulo 3 descreve o estudo que testou a resposta do epifíton à dinâmica do vento (velocidade e direção) durante frentes frias (inverno de 2013) em três situações diferentes: comunidade natural, sucessão em um banco de macrófitas e um mesocosmo (protegido da ação do vento). O epifíton revelou-se resiliente, uma vez que o vento favoreceu a colonização e estabilização, recuperando-se rapidamente após o distúrbio. O mesocosmo diminuiu a ação do vento atrasando a deposição das células metafíticas para início da colonização e a sucessão só começou após um grande distúrbio (vento 10 m s-1) que nivelou a água do mesocosmo e a água circundante. Dominância de diatomáceas foi registrada em todos os experimentos. As diversidades das comunidades de todos os experimentos responderam à dinâmica do vento, sendo mais baixas quando a velocidade do vento excedeu a média encontrada para o período (5 m s -1). / Mangueira Lake is a large shallow subtropical lake located in the Southern Rio Grande do Sul State, Brazil. The lake ranges from oligo to -mesotrophic conditions and presents a large littoral zone covered by macrophytes, providing large area for attached community development. The southern area of the lake is characterized by high transparency and high pH and the northern area is shallower with high concentration of humic acids because of its interaction with the wetland. Due to its shape and geographic position, the lake undergoes constant wind action from NE direction and from SE‒SW during cold-fronts. The wind regulates the hydrodynamics in this environment affecting spatial and temporally the aquatic communities (phytoplankton, zooplankton and fishes), and can be considered a constant disturbance. However, epiphytic algae contribution for food-web, as well as their responses to hydrodynamics, are still a lack. Chapter 1 presents a study in which we tested if epiphytic diatoms respond to the lake spatial heterogeneity during two summers in three distant sites (North, Center and South). As a result we noted that diatoms exhibited a longitudinal gradient from NS whereas the center area assumes characteristics similar to both North and South areas depending on wind dynamics. In Chapter 2, we investigated epiphyton succession during 60 days in southern part of the lake in the summer of 2012, aiming to describe the driving factors of the community development in situ, considering zooplankton as a potential grazer. The study highlighted that the advanced phase of succession was dominated by tightly attached diatoms adapted to high wind mean velocity (±15 m s-1). We also showed that epiphyton was great source of primary production for the food-web and strong correlations proved that the community is handling zooplankton bottom-up control. Chapter 3 describes a study that tested the response of the epiphyton to the wind dynamics (velocity and direction) during cold-fronts (2013 Winter) in three different situations: natural community, succession in a macrophyte bank and in an enclosure (protected from the wind). As a result, epiphyton revealed to be resilient once wind favored colonization and stabilization, rapidly recovering after disturbance. The enclosure acted buffering wind forces delaying succession derived by settlement of metaphytic cells and the succession only started after a huge disturbance (wind 10 m s-1) which leveled the enclosure water and the lake water. Dominance of diatoms was registered in all experiments. The communities‟ diversities responded to wind dynamics, whereas all experiments showed lower diversities when wind velocity exceeded the system‟s means (5 m s-1).

abstract: Climate change will result not only in changes in the mean state of climate but also on changes in variability. However, most studies of the impact of climate change on ecosystems have focused on the effect of changes in the central tendency. The broadest objective of this thesis was to assess the effects of increased interannual precipitation variation on ecosystem functioning in grasslands. In order to address this objective, I used a combination of field experimentation and data synthesis. Precipitation manipulations on the field experiments were carried out using an automated rainfall manipulation system developed as part of this dissertation. Aboveground net primary production responses were monitored during five years. Increased precipitation coefficient of variation decreased primary production regardless of the effect of precipitation amount. Perennial-grass productivity significantly decreased while shrub productivity increased as a result of enhanced precipitation variance. Most interesting is that the effect of precipitation variability increased through time highlighting the existence of temporal lags in ecosystem response. Further, I investigated the effect of precipitation variation on functional diversity on the same experiment and found a positive response of diversity to increased interannual precipitation variance. Functional evenness showed a similar response resulting from large changes in plant-functional type relative abundance including decreased grass and increased shrub cover while functional richness showed non-significant response. Increased functional diversity ameliorated the direct negative effects of precipitation variation on ecosystem ANPP but did not control ecosystem stability where indirect effects through the dominant plant-functional type determined ecosystem stability. Analyses of 80 long-term data sets, where I aggregated annual productivity and precipitation data into five-year temporal windows, showed that precipitation variance had a significant effect on aboveground net primary production that is modulated by mean precipitation. Productivity increased with precipitation variation at sites where mean annual precipitation is less than 339 mm but decreased at sites where precipitation is higher than 339 mm. Mechanisms proposed to explain patterns include: differential ANPP response to precipitation among sites, contrasting legacy effects and soil water distribution. Finally, increased precipitation variance may impact global grasslands affecting plant-functional types in different ways that may lead to state changes, increased erosion and decreased stability that can in turn limit the services provided by these valuable ecosystems. / Dissertation/Thesis / Doctoral Dissertation Biology 2014

Submitted by Maike Costa (maiksebas@gmail.com) on 2016-05-23T12:12:31Z No. of bitstreams: 1 arquivo total.pdf: 1933896 bytes, checksum: b0b1871a7575744c7578f10900648224 (MD5) / Made available in DSpace on 2016-05-23T12:12:31Z (GMT). No. of bitstreams: 1 arquivo total.pdf: 1933896 bytes, checksum: b0b1871a7575744c7578f10900648224 (MD5) Previous issue date: 2015-05-05 / Coordenação de Aperfeiçoamento de Pessoal de Nível Superior - CAPES / Life support in various ecosystems is made possible by a group of organisms, called primary producers. Because they are mostly photoautotrophic, these individuals secure the middle nutrients (in higher carbon part) through the process of photosynthesis. In aquatic ecosystems phytoplankton (microalgae) is responsible for most of the primary production. As a link between individuals of the first trophic level and individuals of higher trophic levels are the organisms belonging to the zooplankton. Thus, the study of the biochemical composition of the zooplankton community can be used as an indicator of nutritional status in aquatic ecosystems. Thus, this study aimed to build PLS multivariate calibration models for the simultaneous spectrofluorimetric determination of neutral and polar lipid classes. In order to simplify the procedures involved in the construction of models, commercial standards were used to perform the calibration mixtures. These solutions were previously defined by conducting a Brereton mixture design of three levels and two factors. In a preliminary step, the models built were used to predict the concentrations of the two commercial lipids, triolein (TO) and phosphatidylcholine (PC), a set of external standards. In evaluating the performance parameters related to this step, it was observed that the models were in an acceptable condition for application to real samples. However, when evaluating the synchronous fluorescence spectra of the samples (macro and mesozooplankton), it was observed that máx the band corresponding to the neutral lipids varies between samples, so that the proposed method can not be applied to real system. / A sustentação da vida nos mais diversos ecossistemas é possibilitada por um grupo de organismos, denominados produtores primários. Por serem em sua maioria fotoautotróficos, esses indivíduos fixam os nutrientes do meio (em maior parte carbono) através do processo de fotossíntese. Nos ecossistemas aquáticos o fitoplâncton (microalgas) é responsável pela maior parcela da produção primária. Como elo entre os indivíduos do primeiro nível trófico e os indivíduos dos níveis tróficos superiores estão os organismos pertencentes ao zooplâncton. Dessa forma, o estudo da composição bioquímica da comunidade zooplanctônica pode ser usado como um indicador da condição nutricional nos ecossistemas aquáticos. Diante disso, este trabalho teve como objetivo a construção de modelos de calibração multivariada PLS para a determinação espectrofluorimétrica simultânea das classes de lipídios neutros e polares. A fim de simplificar o procedimento envolvido na construção dos modelos, foram utilizados padrões comerciais para a realização das misturas de calibração. Estas soluções foram previamente definidas mediante a realização de um planejamento de misturas Brereton de três níveis e dois fatores. Em uma etapa preliminar, os modelos construídos foram usados para prever as concentrações dos dois lipídios comerciais, trioleína (TO) e fosfatidilcolina (PC), em um conjunto de padrões externo. Ao avaliar os parâmetros de desempenho relacionados a esta etapa, observou-se que os modelos estavam em condições aceitáveis para aplicação em amostras reais. Entretanto, ao avaliar os espectros de fluorescência sincrônica das amostras analisadas (macro e mesozooplâncton), foi observado que máx da banda correspondente aos lipídios neutros varia entre as amostras, de modo que o método proposto não pode ser aplicado em sistema real.

A Lagoa Mangueira é uma extensa lagoa rasa subtropical localizada no sul do estado do Rio Grande do Sul, Brasil. É um ambiente que varia de oligo à mesotrófico, com extensa área litoral colonizada por macrófitas aquáticas, fornecendo grande área de substrato para o desenvolvimento da comunidade aderida. A parte sul da lagoa é caracterizada pela alta transparência e alto pH, e a parte norte é mais rasa com alta concentração de ácidos húmicos devido a interação com banhado. Devido sua forma e posição geográfica a lagoa sofre constante ação dos ventos de direção NE, com mudanças para o quadrante SE-SO durante as frentes frias. O vento neste ambiente é um fator regulador da hidrodinâmica, afetando espacial e temporalmente as comunidades aquáticas (fitoplâncton, zooplâncton, e peixes), podendo ser considerado um distúrbio constante no ambiente. Entretanto, ainda há uma lacuna quanto à contribuição das algas epifíticas para a teia trófica, bem como a resposta dessa comunidade à hidrodinâmica. O Capítulo 1 apresenta um estudo que testou se as diatomáceas epifíticas respondem a heterogeneidade espacial da lagoa durante dois verões em três pontos distantes (Norte, Centro e Sul). Verificou-se que as diatomáceas exibem um gradiente longitudinal NS e a área central assume características semelhantes aos pontos Norte e Sul dependendo da dinâmica do vento. No Capítulo 2, foi avaliada a sucessão das algas epifíticas no Sul da lagoa, durante 60 dias no verão de 2012, para investigar os fatores reguladores do desenvolvimento da comunidade in situ, considerando o zooplâncton como potencial predador. O estudo evidenciou que a fase avançada da sucessão foi dominada por diatomáceas fortemente aderidas e adaptadas às altas médias da velocidade do vento (±15 m s-1). O epifíton mostrou-se uma grande fonte de produtividade primária para a teia trófica e fortes correlações provaram que a comunidade sustentou o controle bottom-up da comunidade zooplanctônica. O Capítulo 3 descreve o estudo que testou a resposta do epifíton à dinâmica do vento (velocidade e direção) durante frentes frias (inverno de 2013) em três situações diferentes: comunidade natural, sucessão em um banco de macrófitas e um mesocosmo (protegido da ação do vento). O epifíton revelou-se resiliente, uma vez que o vento favoreceu a colonização e estabilização, recuperando-se rapidamente após o distúrbio. O mesocosmo diminuiu a ação do vento atrasando a deposição das células metafíticas para início da colonização e a sucessão só começou após um grande distúrbio (vento 10 m s-1) que nivelou a água do mesocosmo e a água circundante. Dominância de diatomáceas foi registrada em todos os experimentos. As diversidades das comunidades de todos os experimentos responderam à dinâmica do vento, sendo mais baixas quando a velocidade do vento excedeu a média encontrada para o período (5 m s -1). / Mangueira Lake is a large shallow subtropical lake located in the Southern Rio Grande do Sul State, Brazil. The lake ranges from oligo to -mesotrophic conditions and presents a large littoral zone covered by macrophytes, providing large area for attached community development. The southern area of the lake is characterized by high transparency and high pH and the northern area is shallower with high concentration of humic acids because of its interaction with the wetland. Due to its shape and geographic position, the lake undergoes constant wind action from NE direction and from SE‒SW during cold-fronts. The wind regulates the hydrodynamics in this environment affecting spatial and temporally the aquatic communities (phytoplankton, zooplankton and fishes), and can be considered a constant disturbance. However, epiphytic algae contribution for food-web, as well as their responses to hydrodynamics, are still a lack. Chapter 1 presents a study in which we tested if epiphytic diatoms respond to the lake spatial heterogeneity during two summers in three distant sites (North, Center and South). As a result we noted that diatoms exhibited a longitudinal gradient from NS whereas the center area assumes characteristics similar to both North and South areas depending on wind dynamics. In Chapter 2, we investigated epiphyton succession during 60 days in southern part of the lake in the summer of 2012, aiming to describe the driving factors of the community development in situ, considering zooplankton as a potential grazer. The study highlighted that the advanced phase of succession was dominated by tightly attached diatoms adapted to high wind mean velocity (±15 m s-1). We also showed that epiphyton was great source of primary production for the food-web and strong correlations proved that the community is handling zooplankton bottom-up control. Chapter 3 describes a study that tested the response of the epiphyton to the wind dynamics (velocity and direction) during cold-fronts (2013 Winter) in three different situations: natural community, succession in a macrophyte bank and in an enclosure (protected from the wind). As a result, epiphyton revealed to be resilient once wind favored colonization and stabilization, rapidly recovering after disturbance. The enclosure acted buffering wind forces delaying succession derived by settlement of metaphytic cells and the succession only started after a huge disturbance (wind 10 m s-1) which leveled the enclosure water and the lake water. Dominance of diatoms was registered in all experiments. The communities‟ diversities responded to wind dynamics, whereas all experiments showed lower diversities when wind velocity exceeded the system‟s means (5 m s-1).

A Lagoa Mangueira é uma extensa lagoa rasa subtropical localizada no sul do estado do Rio Grande do Sul, Brasil. É um ambiente que varia de oligo à mesotrófico, com extensa área litoral colonizada por macrófitas aquáticas, fornecendo grande área de substrato para o desenvolvimento da comunidade aderida. A parte sul da lagoa é caracterizada pela alta transparência e alto pH, e a parte norte é mais rasa com alta concentração de ácidos húmicos devido a interação com banhado. Devido sua forma e posição geográfica a lagoa sofre constante ação dos ventos de direção NE, com mudanças para o quadrante SE-SO durante as frentes frias. O vento neste ambiente é um fator regulador da hidrodinâmica, afetando espacial e temporalmente as comunidades aquáticas (fitoplâncton, zooplâncton, e peixes), podendo ser considerado um distúrbio constante no ambiente. Entretanto, ainda há uma lacuna quanto à contribuição das algas epifíticas para a teia trófica, bem como a resposta dessa comunidade à hidrodinâmica. O Capítulo 1 apresenta um estudo que testou se as diatomáceas epifíticas respondem a heterogeneidade espacial da lagoa durante dois verões em três pontos distantes (Norte, Centro e Sul). Verificou-se que as diatomáceas exibem um gradiente longitudinal NS e a área central assume características semelhantes aos pontos Norte e Sul dependendo da dinâmica do vento. No Capítulo 2, foi avaliada a sucessão das algas epifíticas no Sul da lagoa, durante 60 dias no verão de 2012, para investigar os fatores reguladores do desenvolvimento da comunidade in situ, considerando o zooplâncton como potencial predador. O estudo evidenciou que a fase avançada da sucessão foi dominada por diatomáceas fortemente aderidas e adaptadas às altas médias da velocidade do vento (±15 m s-1). O epifíton mostrou-se uma grande fonte de produtividade primária para a teia trófica e fortes correlações provaram que a comunidade sustentou o controle bottom-up da comunidade zooplanctônica. O Capítulo 3 descreve o estudo que testou a resposta do epifíton à dinâmica do vento (velocidade e direção) durante frentes frias (inverno de 2013) em três situações diferentes: comunidade natural, sucessão em um banco de macrófitas e um mesocosmo (protegido da ação do vento). O epifíton revelou-se resiliente, uma vez que o vento favoreceu a colonização e estabilização, recuperando-se rapidamente após o distúrbio. O mesocosmo diminuiu a ação do vento atrasando a deposição das células metafíticas para início da colonização e a sucessão só começou após um grande distúrbio (vento 10 m s-1) que nivelou a água do mesocosmo e a água circundante. Dominância de diatomáceas foi registrada em todos os experimentos. As diversidades das comunidades de todos os experimentos responderam à dinâmica do vento, sendo mais baixas quando a velocidade do vento excedeu a média encontrada para o período (5 m s -1). / Mangueira Lake is a large shallow subtropical lake located in the Southern Rio Grande do Sul State, Brazil. The lake ranges from oligo to -mesotrophic conditions and presents a large littoral zone covered by macrophytes, providing large area for attached community development. The southern area of the lake is characterized by high transparency and high pH and the northern area is shallower with high concentration of humic acids because of its interaction with the wetland. Due to its shape and geographic position, the lake undergoes constant wind action from NE direction and from SE‒SW during cold-fronts. The wind regulates the hydrodynamics in this environment affecting spatial and temporally the aquatic communities (phytoplankton, zooplankton and fishes), and can be considered a constant disturbance. However, epiphytic algae contribution for food-web, as well as their responses to hydrodynamics, are still a lack. Chapter 1 presents a study in which we tested if epiphytic diatoms respond to the lake spatial heterogeneity during two summers in three distant sites (North, Center and South). As a result we noted that diatoms exhibited a longitudinal gradient from NS whereas the center area assumes characteristics similar to both North and South areas depending on wind dynamics. In Chapter 2, we investigated epiphyton succession during 60 days in southern part of the lake in the summer of 2012, aiming to describe the driving factors of the community development in situ, considering zooplankton as a potential grazer. The study highlighted that the advanced phase of succession was dominated by tightly attached diatoms adapted to high wind mean velocity (±15 m s-1). We also showed that epiphyton was great source of primary production for the food-web and strong correlations proved that the community is handling zooplankton bottom-up control. Chapter 3 describes a study that tested the response of the epiphyton to the wind dynamics (velocity and direction) during cold-fronts (2013 Winter) in three different situations: natural community, succession in a macrophyte bank and in an enclosure (protected from the wind). As a result, epiphyton revealed to be resilient once wind favored colonization and stabilization, rapidly recovering after disturbance. The enclosure acted buffering wind forces delaying succession derived by settlement of metaphytic cells and the succession only started after a huge disturbance (wind 10 m s-1) which leveled the enclosure water and the lake water. Dominance of diatoms was registered in all experiments. The communities‟ diversities responded to wind dynamics, whereas all experiments showed lower diversities when wind velocity exceeded the system‟s means (5 m s-1).

Tese(doutorado) - Universidade Federal do Rio Grande, Programa de Pós–Graduação em Oceanografia Biológica, Instituto de Oceanografia, 2011. / Submitted by Cristiane Gomides (cristiane_gomides@hotmail.com) on 2013-11-07T10:21:14Z No. of bitstreams: 1 Alessandro Pereira Cardozo.pdf: 690453 bytes, checksum: f71211cfed3997fedfee94740de5f4d6 (MD5) / Rejected by cristiane soares (krikasoares@live.com), reason: Citação errada on 2013-11-13T19:57:55Z (GMT) / Submitted by Cristiane Gomides (cristiane_gomides@hotmail.com) on 2013-11-18T12:13:07Z No. of bitstreams: 1 Alessandro Pereira Cardozo.pdf: 690453 bytes, checksum: f71211cfed3997fedfee94740de5f4d6 (MD5) / Approved for entry into archive by Angelica Miranda (angelicacdm@gmail.com) on 2013-11-18T18:52:43Z (GMT) No. of bitstreams: 1 Alessandro Pereira Cardozo.pdf: 690453 bytes, checksum: f71211cfed3997fedfee94740de5f4d6 (MD5) / Made available in DSpace on 2013-11-18T18:52:43Z (GMT). No. of bitstreams: 1 Alessandro Pereira Cardozo.pdf: 690453 bytes, checksum: f71211cfed3997fedfee94740de5f4d6 (MD5) Previous issue date: 2011 / O desenvolvimento acelerado da atividade de aquacultura ao longo dos últimos anos levou ao desenvolvimento de novas tecnologias em busca de sistemas mais produtivos e ao mesmo tempo ecologicamente corretos. O objetivo desta tese foi o de avaliar o impacto dos cultivos de camarões na comunidade planctônica, na região do estuário da Lagoa dos Patos. A dinâmica das comunidades planctônicas em viveiros de cultivo de Litopenaeus vannamei foi comparada ao estuário em áreas adjacentes ao cultivo, indicando que no ambiente de cultivo a disponibilidade de nutrientes (bottomup) e o efeito predação top-down influenciam a estrutura de tamanho e composição do fitoplâncton. O impacto da descarga de efluente proveniente do cultivo de camarões foi testado em microcosmos, simulando condições que ocorreriam na região estuarina, e pode-se concluir que a descarga deste efluente pode levar a alterações de curto prazo no ambiente receptor, especialmente na taxa de produção primária e teor de clorofila-a. Fertilizações com compostos ricos em carbono, que tendem a aumentar a produtividade através de cultivos em sistema com tecnologia de bioflocos (BFT), são utilizadas com freqüência no cultivo de camarões marinhos. Na avaliação da influência da adição de fonte de carbono sobre o plâncton e produção primária em viveiros, observou-se que o teor de clorofila, taxa de produção primária e concentração de zooplâncton, ao contrário do esperado, foram similares entre viveiros testados com e sem fertilização. / Aquaculture activities accelerated fast in the past years and led to the development of new technologies in search of more productive and environmentally correct systems. The objective of this thesis was to evaluate the impact of shrimp farming in the plankton community at the Patos Lagoon estuary region. Plankton community dynamics in culture earthen ponds of Litopenaeus vannamei, compared to those of the estuarine area adjacent to the shrimp farm, showed that in the ponds both controls the nutrient availability bottom-up and the predation top-down influence the size structure and composition of phytoplankton. The impact of effluent discharge from shrimp ponds has been tested in microcosms experiments, simulating conditions that would occur in the estuarine region. It is concluded that the effluent discharge can lead to short-term changes in the environment, especially in the primary production rate and chlorophyll-a levels. Fertilizations with carbon rich compounds, which tend to increase the productivity of the Biofloc Technology system, are commonly used in the culture of marine shrimp. In assessing the influence of the addition of a carbon source on the phytoplankton and primary production rate in the ponds, it was observed that the chlorophyll a levels, primary production rate and zooplankton concentration, unexpectedly, were similar between ponds with and without carbon fertilization.

L'océan Austral aux moyennes latitudes est l'un des endroits les plus tempétueux sur Terre. On peut donc supposer que le passage de ces tempêtes intenses sur cette variabilité océanique intense peut avoir un impact fort sur la variabilité intra-saisonnière des couches de surface où vit le phytoplancton. Pour autant, cet impact sur le taux de croissance du phytoplancton et sa variabilité reste encore très méconnu. C'est à cette question que s'efforce de répondre ce travail de thèse visant à faire progresser la compréhension de la variabilité intra-saisonnière de la production primaire de l'océan Austral. Nos expériences de modélisation suggèrent que les apports en Fer dissous (DFe) dans les eaux de surface à l'échelle intra-saisonnière par les tempêtes jouent un rôle bien plus actif et déterminant qu'on ne le pensait pour expliquer la productivité estivale importante de l'océan Austral. Deux idées importantes ressortent: 1. Les interactions tempête-tourbillon peuvent fortement augmenter l'amplitude et l'extension du mélange vertical agissant sur des couches traditionnellement considérées comme superficielles, mais également en subsurface. Ces deux régimes de mélange possèdent des dynamiques différentes mais agissent de concert pour augmenter les flux de DFe à la surface des océans. 2. Les tempêtes génèrent des courants inertiels qui peuvent considérablement renforcer les vitesses verticales w par interaction avec les tourbillons. Cela favorise l'advection verticale de DFe à la surface de l'océan, et avoir un effet plusieurs jours après la tempête. Ces interactions entre les tempêtes et les tourbillons peuvent considérablement intensifier la variabilité production primaire. / The Southern Ocean is one of the stormiest places on earth; here strong mid-latitude storms frequently traverse large distances of this ocean. The presence of the passage of intense storms and meso to sub-mesoscale eddy variability has the potential to strongly impact the intra-seasonal variability of the upper ocean environment where phytoplankton live. Yet, exactly how phytoplankton growth rates and its variability are impacted by the dominance of such features is not clear. Herein, lies the problem addressed by the core of this thesis, which seeks to advance the understanding of intra-seasonal variability of Southern Ocean primary production. Model experiments have suggested that intra-seasonal storm-linked physical supplies of dissolved iron (DFe) during the summer played a considerably more active and influential role in explaining the sustained summer productivity in the surface waters of the Southern Ocean than what was thought previously. This was through two important insights: 1. Storm-eddy interactions may strongly enhance the magnitude and extent of upper-ocean vertical mixing in both the surface mixed layer as traditionally understood as well as in the subsurface ocean. These two mixing regimes have different dynamics but act in concert to amplify the DFe fluxes to the surface ocean. 2. Storm initiated inertial motions may, through interaction with eddies, greatly reinforce w and thus, enhance the vertical advection of DFe to the surface ocean, an effect that may last several days after the storm. Such storm-eddy dynamics may greatly increase the intra-seasonal variability of primary production.

Understanding the mechanism of photosynthetic seasonality in Amazonian evergreen forests is critical for its formulation in global climate and carbon cycle models. However, the control of the unexpected photosynthetic seasonality is highly uncertain. Here we use eddy-covariance data across a network of Amazonian research sites and a novel evapotranspiration (E) and two-leaf-photosynthesis-coupled model to investigate links between photosynthetic seasonality and climate factors on monthly scales. It reproduces the GPP seasonality (R-2=0.45-0.69) with a root-mean-square error (RMSE) of 0.67-1.25gCm(-2)d(-1) and a Bias of -0.03-1.04gCm(-2)d(-1) for four evergreen forest sites. We find that the proportion of diffuse and direct sunlight governs the photosynthetic seasonality via their interaction with sunlit and shaded leaves, supported by a proof that canopy light use efficiency (LUE) has a strong linear relationship with the fraction of diffuse sunlight for Amazonian evergreen forests. In the transition from dry season to rainy season, incident total radiation (Q) decreased while LUE and diffuse fraction increased, which produced the large seasonal increase (similar to 34%) in GPP of evergreen forests. We conclude that diffuse radiation is an important environmental driver of the photosynthetic seasonality in tropical Amazon forests yet depending on light utilization by sunlit and shaded leaves. Besides, the GPP model simulates the precipitation-dominated GPP seasonality (R-2=0.40-0.69) at pasture and savanna sites. These findings present an improved physiological method to relate light components with GPP in tropical Amazon. Plain Language Summary Understanding the mechanism of photosynthetic seasonality in Amazonian evergreen forests is critical for its formulation in global climate and carbon cycle models. However, the control of the unexpected photosynthetic seasonality is highly uncertain. Here we use eddy-covariance data across a network of Amazonian research sites and a novel evapotranspiration (E) and two-leaf-photosynthesis-coupled model to investigate links between photosynthetic seasonality and climate factors on monthly scales. It reproduces the GPP seasonality (R2= 0.45-0.69) for four evergreen forest sites. We find that the proportion of diffuse and direct sunlight governs the photosynthetic seasonality via their interaction with sunlit and shaded leaves, supported by a proof that canopy light-use efficiency (LUE) has a strong linear relationship with the fraction of diffuse sunlight for Amazonian evergreen forests. We conclude that diffuse radiation is an important environmental driver of the photosynthetic seasonality in tropical Amazon forests yet depending on light utilization by sunlit and shaded leaves. Besides, the GPP model simulates the precipitation-dominated GPP seasonality (R2= 0.40 similar to 0.69) at pasture and savanna sites. These findings present an improved physiological method to relate light components with GPP in Amazon.