No description available.

No description available.

The purpose of this study was to determine the prevalence and demographic predictors of pulpal and/or periradicular disease in an urban population. A total of 210 subjects were recruited from a population of patients that were screened for acceptance to the dental school clinics. The diagnosis of pulpal and/or periradicular disease was made using the following data: radiographic interpretation, patient's history of previous pain and chief complaint, and objective pulpal testing. Objective pulpal testing included percussion, palpation, electric pulp test, and cold. The unit of observation was the individual, not the tooth. The overall prevalence of endodontic disease among the study sample of the screening patient population was 39.52%. Controlling for gender, patients in the 30-39 age group were 3.05 times more likely to have pulpal disease than patients in the 18-29 age group (OR=3.05, 95%CI 1.04-8.9). Controlling for age, men were 1.82 times more likely to have pulpal disease than women (OR=1.82, 95%CI 1.01-3.26). Non-white patients were 2.69 times more likely to have pulpal disease than white patients (OR=2.69, 95%CI 1.51-4.81). Patients who earned less than $25,000 were 2.06 times more likely to have pulpal disease compared to those who earned more than $25,000 (OR=2.06, 95% CI 1.15-3.69). Overall, this data provides valuable information for identifying vulnerable populations and addressing the policy goals of the U.S. Surgeon General.

Made available in DSpace on 2014-06-11T19:33:02Z (GMT). No. of bitstreams: 0 Previous issue date: 2004Bitstream added on 2014-06-13T21:05:43Z : No. of bitstreams: 1 oliveira_mf_dr_arafo.pdf: 1636910 bytes, checksum: 92e89d2ea64aaa089a1ab0dc86bba68e (MD5) / Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) / Esta pesquisa teve como objetivo avaliar a resposta do tecido pulpar de dentes de macacos-prego exposto mecanicamente e capeado com proteínas bioativas extraídas da dentina de incisivos de coelhos ou pasta de hidróxido de cálcio, precedidas ou não de uma aplicação do laser de baixa intensidade. Para o experimento foram selecionados 36 dentes de 4 macacos-prego, os quais foram divididos aleatoriamente nos seguintes grupos: G1: pasta de hidróxido de cálcio em água destilada, G2: laser + pasta de hidróxido de cálcio em água destilada, G3: proteína bioativa, G4: laser + proteína bioativa, G5: laser e G6: controle hígido. Cavidades classe V foram confeccionadas na face vestibular dos dentes. Após exposição mecânica da polpa, diferentes terapias foram realizadas de acordo com os grupos experimentais. As cavidades foram restauradas com cimento de óxido de zinco e eugenol e amálgama. Decorrido o período experimental de 180 dias, os animais foram sacrificados, os dentes removidos e preparados para análise histológica. Os resultados mostraram que não houve diferença estatisticamente significante entre os grupos experimentais para os eventos histopatológicos resposta celular inflamatória, desorganização tecidual e presença de bactérias coradas. No entanto, em relação à formação de barreira mineralizada, o grupo que recebeu como material capeador a pasta de hidróxido de cálcio precedida ou não da aplicação do laser apresentou as melhores respostas com formação de barreiras mineralizadas espessas e homogêneas. Concluiu-se que o laser de baixa intensidade aplicado previamente ao capeamento pulpar direto, não melhorou de forma significativa a resposta do tecido pulpar aos materiais capeadores empregados. A pasta de hidróxido de cálcio, aplicada sobre as polpas expostas, caracterizou os melhores resultados de reparação tecidual quando comparada às proteínas... / The aim of this in vivo study was to evaluate the response of the dental pulp capped with bioactive proteins extracted from rabbit incisors dentin or calcium hydroxide paste, associated or not to a low energy laser irradiation. Thirty-six teeth of four non-human primates were randomly divided in the following groups: G1: calcium hydroxide paste; G 2: laser + calcium hydroxide paste; G3: bioactive proteins; G4: laser + bioactive proteins; G5: laser; G6: sound teeth (control). Deep class V cavities were prepared on the buccal surface of the teeth. The pulp tissue was mechanically exposed and capped with the experimental materials. The cavities were filled-up with zinc-oxide and eugenol cement and amalgam. After the postoperative period of 180 days, the animals were sacrificed and the teeth processed for histological evaluation. The histopathological analysis showed no statistical difference among the experimental groups regarding to the inflammatory cell response, tissue disorganization and stained bacteria. However, pulps capped with calcium hydroxide, regardless the low-energy laser irradiation, exhibited the best responses, with thick and homogeneous hard tissue barriers. It was concluded that the low-energy laser irradiation preceding the direct pulp capping did not improve the pulp tissue healing and dentin matrix deposition. Pulps capped with calcium hydroxide paste showed better results when compared to those capped with bioactive proteins regarding to the hard tissue barrier formation.

Orientador: Elisa Maria Aparecida Giro / Resumo: Esta pesquisa teve como objetivo avaliar a resposta do tecido pulpar de dentes de macacos-prego exposto mecanicamente e capeado com proteínas bioativas extraídas da dentina de incisivos de coelhos ou pasta de hidróxido de cálcio, precedidas ou não de uma aplicação do laser de baixa intensidade. Para o experimento foram selecionados 36 dentes de 4 macacos-prego, os quais foram divididos aleatoriamente nos seguintes grupos: G1: pasta de hidróxido de cálcio em água destilada, G2: laser + pasta de hidróxido de cálcio em água destilada, G3: proteína bioativa, G4: laser + proteína bioativa, G5: laser e G6: controle hígido. Cavidades classe V foram confeccionadas na face vestibular dos dentes. Após exposição mecânica da polpa, diferentes terapias foram realizadas de acordo com os grupos experimentais. As cavidades foram restauradas com cimento de óxido de zinco e eugenol e amálgama. Decorrido o período experimental de 180 dias, os animais foram sacrificados, os dentes removidos e preparados para análise histológica. Os resultados mostraram que não houve diferença estatisticamente significante entre os grupos experimentais para os eventos histopatológicos resposta celular inflamatória, desorganização tecidual e presença de bactérias coradas. No entanto, em relação à formação de barreira mineralizada, o grupo que recebeu como material capeador a pasta de hidróxido de cálcio precedida ou não da aplicação do laser apresentou as melhores respostas com formação de barreiras mineralizadas espessas e homogêneas. Concluiu-se que o laser de baixa intensidade aplicado previamente ao capeamento pulpar direto, não melhorou de forma significativa a resposta do tecido pulpar aos materiais capeadores empregados. A pasta de hidróxido de cálcio, aplicada sobre as polpas expostas, caracterizou os melhores resultados de reparação tecidual quando comparada às proteínas... (Resumo completo, clicar acesso eletrônico abaixo) / Abstract: The aim of this in vivo study was to evaluate the response of the dental pulp capped with bioactive proteins extracted from rabbit incisors dentin or calcium hydroxide paste, associated or not to a low energy laser irradiation. Thirty-six teeth of four non-human primates were randomly divided in the following groups: G1: calcium hydroxide paste; G 2: laser + calcium hydroxide paste; G3: bioactive proteins; G4: laser + bioactive proteins; G5: laser; G6: sound teeth (control). Deep class V cavities were prepared on the buccal surface of the teeth. The pulp tissue was mechanically exposed and capped with the experimental materials. The cavities were filled-up with zinc-oxide and eugenol cement and amalgam. After the postoperative period of 180 days, the animals were sacrificed and the teeth processed for histological evaluation. The histopathological analysis showed no statistical difference among the experimental groups regarding to the inflammatory cell response, tissue disorganization and stained bacteria. However, pulps capped with calcium hydroxide, regardless the low-energy laser irradiation, exhibited the best responses, with thick and homogeneous hard tissue barriers. It was concluded that the low-energy laser irradiation preceding the direct pulp capping did not improve the pulp tissue healing and dentin matrix deposition. Pulps capped with calcium hydroxide paste showed better results when compared to those capped with bioactive proteins regarding to the hard tissue barrier formation. / Doutor

No description available.

The purpose of this study was to test the predictive ability of specific questions in diagnosis of pulpal and periradicular disease in a dental school population. 210 patients were recruited to fill out a questionnaire, and undergo a clinical examination for pulpal and/or periradicular disease.The Questionnaire asked if the patient had in the last 6 months: 1. a toothache that kept them up at night 2. a toothache that required pain killers 3. facial swelling caused by a tooth 4. injury to any teeth 5. a broken tooth 6. a tooth darker in color than the surrounding teeth 7. a bump, pimple, or boil on the gums. 8. a toothache after eating or drinking something hot or cold 9. repeated sharp pain in the same area while chewing 10. A toothache or facial swelling that caused them to visit an emergency room.Examiners were blinded to the responses on the questionnaire and then, based on the clinical examination and testing, determined the presence or absence of endodontic disease. The data was analyzed using univariate logistic regression models.Results: The most predictive questions were #1 and #2 (p-value Conclusion: Patients with pain that wakes them at night or that requires analgesics were greater than 3 times more likely to have endodontic disease at the time of examination.

Symptomatic apical periodontitis (SAP) is indicative of inflammation of the periodontal ligament. It may or may not be accompanied by radiographic changes and may occur with vital or necrotic pulpal diagnosis. Purpose of this study was to analyze clinical and radiographic presentations of SAP in a retrospective electronic dental chart and digital radiograph review utilizing the endodontic diagnostic template note of predoctoral dental school patients presenting with SAP. The aim was to determine prevalence of periapical radiolucencies (PARLs) in SAP, association of SAP to pulpal diagnosis, and define associated radiographic changes. Most prevalent pulpal diagnosis with SAP was symptomatic irreversible pulpitis (44%). A tooth presenting with SAP was more likely to have an intact lamina dura, but presented with a PARL 38% of the time. When a PARL was present the most common pulpal diagnosis was pulp necrosis however, 24.5% of teeth presented with a vital pulp diagnosis and lesions <2mm.

Alterações celulares e neurovasculares na polpa têm sido relacionadas a forças ortodónticas, usadas na movimentação dentária. As alterações observadas são geralmente relacionadas a alterações vasculares na polpa. Os primeiros estudos sobre alterações do fluxo sangüíneo pulpar (FSP) durante a movimentação dentária foram realizados usando técnicas qualitativas (e. g., cortes histológicos), e quantitativas (e. g., micro-esferas radioativas ou fluorescentes, radioespirometria). Tais técnicas são invasivas, destrutivas e não permitem o estudo de alterações dinâmicas do fluxo. Recentemente a fluxometria laser Doppler (FLD), uma técnica não invasiva, tem sido usada no estudo de alterações dinâmicas do FSP em humanos em condições próximas às reais. Mas os estudos disponíveis na literatura, usando a FLD, ainda são limitados a movimentos experimentais, pois ainda existem limitações práticas na FLD. O objetivo desta pesquisa foi, usando a FLD, estudar alterações do FSP de voluntários submetidos a duas fases de um tratamento ortodôntico corrente (nivelamento e retração), dentro de condições clinicas reais. Doze voluntários foram monitorados durante a fase de nivelamento e treze voluntários foram monitorados durante a fase de retração, antes da aplicação de forças, 20 minutos, 48h, 72h e 1 mês depois de aplicadas as forças. Analisando os resultados obtidos, durante as duas fases estudas foram encontradas evidências estatísticas suficientes para inferir que ocorrem alterações significativas no FSP, quando medidas via FLD, durante os movimentos e forças estudadas, nos seguintes momentos investigados: diminuição do FSP nos momentos 20 minutos, 48h e 72h. Durante o nivelamento, no dia 30 o FSP é próximo ou igual ao basal. Durante a retração, no dia 30 o fluxo ainda permanece diminuído. / Cellular and neurovascular changes in the pulp have been related to orthodontic forces, used to produce a dental movement. The observed alterations are generally related to pulpal vascular changes. Earlier studies on pulpal blood flow (PBF) changes during the dental movement were carried out by using qualitative techniques (e. g., histological slices), and quantitative (e. g., radioactive or fluorescent microspheres, radiospirometry). Such techniques are invasive, destructive, and do not allow dynamic flow changes to be studied. Recently the laser Doppler flowmetry (LDF), a non-invasive technique, has been used to study dynamic PBF alterations in humans into nearly real conditions. The available studies in the literature, by using the LDF, are limited to experimental studies, probably due to the LDF practical limitations. The aim of this work was, by using the FDF, to study PBF alterations from volunteers subjected to two phases of a current orthodontic treatment (leveling and retraction), into real clinical conditions. Twelve volunteers were monitored during the leveling phase and thirteen volunteers were monitored during the retraction phase, before the force to be applied, and 20 minutes, 48 hr, 72 hr and 1 month later. Analyzing the obtained results, during the two studied phases, it were found suffice statistical evidences to infer that significant PBF decrease occurs on the investigates moments: 20 minutes, 48 hr and 72 hr. During the leveling phase, on day 30, the PBF is near or equal to the basal level. During the retraction, on day 30, the flow is still decreased.

Orientador: Rogério de Castilho Jacinto / Banca: João Eduardo Gomes Filho / Banca: Paulo Carvalho Tobias Duarte / Resumo: Este estudo teve como objetivo comparar quantitativamente a diferença de expressão proteica na progressão da patogênese pulpar, bem como correlacionar as funções biológicas das proteínas identificadas no tecido pulpar normal, inflamado ou necrótico. As amostras foram obtidas de pacientes atendidos na Clínica Endodôntica da Faculdade de Odontologia de Araçatuba para tratamento endodôntico, sendo divididos em três grupos: grupo de polpa normal, com amostras do tecido pulpar obtidas a partir de dentes extraídos por indicação ortodôntica (n = 2); grupo de polpa inflamada, com amostras obtidas de pacientes com diagnóstico de pulpite irreversível (n = 2) e grupo de polpa necrótica, cujas amostras foram obtidas de pacientes com diagnóstico de periodontite apical crônica (n = 2). Após o preparo proteômico prévio, as amostras de polpa dentária foram processadas para análise proteômica quantitativa livre de marcadores em um sistema nanoACQUITY UPLC-Xevo QTof MS. A diferença na expressão entre os grupos de polpa normal e inflamada e grupos de polpa inflamada e necrótica foi calculada com o software Protein Lynx Global Service, usando o algoritmo Monte-Carlo, e expressa como p <0,05 para proteínas presentes em menor abundância e 1-p> 0,95 para proteínas presentes em maior abundância. Um total de 465 proteínas humanas foram identificadas em todos os grupos. Nos grupos normal, inflamado e necrótico, foram encontradas 241, 240 e 124 proteínas, respectivamente. Na análise quantitativa, as pr... (Resumo completo, clicar acesso eletrônico abaixo) / Abstract: This study aimed to quantitatively compare the difference in protein expression in the progression of pulp pathogenesis, as well as to correlate the biological functions of proteins identified in normal, inflamed or necrotic pulp tissue. The samples were obtained from patients treated at the Endodontic Clinic of the Araçatuba Dental School for endodontic treatment, and were divided into three groups: normal pulp group with pulp tissue samples obtained from orthodontic teeth (n = 2) ; inflamed pulp group, whose samples were obtained from patients diagnosed with irreversible pulpitis (n = 2) and necrotic pulp group, whose samples were obtained from patients diagnosed with chronic apical periodontitis (n = 2). After previous proteomic preparation, dental pulp samples were processed for label-free quantitative proteomic analysis in a nanoACQUITY UPLC-Xevo QTof MS system. The difference in expression between the normal and inflamed pulp groups and groups of inflamed and necrotic pulp was calculated using the Protein Lynx Global Service software using the Monte Carlo algorithm and expressed as p <0.05 for proteins present in lower abundance and 1-p> 0.95 for proteins present in greater abundance. A total of 465 human proteins were identified in all groups. In the normal, inflamed and necrotic groups, 241, 240 and 124 proteins were found, respectively. In the quantitative analysis, the most expressed proteins were hemoglobin, peroxiredoxins and immunoglobulins, whereas the less expressed were the tubulins in the inflamed pulp group in relation to the normal pulp group. Expression of albumins, immunoglobulins and alpha-2-macroglobulin were increased in the necrotic pulp group when compared to normal pulp, whereas hemoglobin and actin were less expressed. As for the qualitative analysis, the proteins identified in the normal... (Complete abstract electronic access below) / Mestre